FastQCFastQC Report
Fri 17 Jun 2016
SRR1526273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36059
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9642.673396378158019No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2860.7931445686236447No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2560.7099475859008847No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2010.5574197842424915No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG830.23017831886630244No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG620.1719404309603705No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG600.16639396544551985No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.1442081033861172No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC500.13866163787126654No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG450.12479547408413988No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA430.11924900856928923No Hit
CAACAAGGGGAGTTCTTTGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTG390.10815607753958789No Hit
CAACAAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG390.10815607753958789No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC150.00812637750.36694738
AGAACCT150.00812637750.36694740
CGTCTCA550.050.36694741
TATGCTC451.2369128E-1050.2262635
TGCTCGT451.2369128E-1050.2262637
ATGCTCG451.2369128E-1050.2262636
CTCTGGC150.00821691150.22625737
CGGAGAA150.00821691150.22625737
TATGCCT150.00821691150.22625735
TTTTCCA150.00821691150.22625736
ATGCCTG150.00821691150.22625736
ATACACA150.00821691150.22625736
TATGCTT1950.050.22625435
ATATGCT2550.050.15620834
ATATGCC451.2551027E-1050.15620834
CAAGATT204.0945332E-450.08635347
GTGCAAT204.0945332E-450.08635349
AACTCTG150.00830819450.08635347
ACTTTCG150.00830819450.08635345
AAGATTC150.00830819450.08635348
Produced by FastQC (version 0.11.5)