##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526273_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36059 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.66762805402257 39.0 38.0 40.0 35.0 40.0 2 36.83954075265537 39.0 38.0 40.0 33.0 40.0 3 36.36881222440999 39.0 38.0 40.0 31.0 40.0 4 36.8374608280873 39.0 37.0 40.0 32.0 40.0 5 36.15541196372612 39.0 38.0 39.0 29.0 40.0 6 34.731745195374245 39.0 36.0 39.0 18.0 40.0 7 36.19917357663829 38.0 36.0 39.0 30.0 40.0 8 36.390637566210934 38.0 36.0 39.0 30.0 40.0 9 36.33486785545911 38.0 36.0 39.0 31.0 40.0 10 35.59485842646773 38.0 35.0 39.0 28.0 40.0 11 34.89037410909898 38.0 33.0 39.0 27.0 40.0 12 32.76682658975568 37.0 30.0 39.0 18.0 40.0 13 32.54618819157492 36.0 30.0 39.0 18.0 40.0 14 32.63135416955545 37.0 30.0 39.0 17.0 40.0 15 32.74647106131618 37.0 30.0 39.0 18.0 40.0 16 33.00374386422252 37.0 31.0 39.0 19.0 40.0 17 32.99769821681134 37.0 30.0 39.0 20.0 40.0 18 32.51654233339804 36.0 30.0 39.0 18.0 40.0 19 33.36756426966915 36.0 31.0 39.0 23.0 40.0 20 33.37574530630356 36.0 31.0 39.0 23.0 40.0 21 34.35563936881223 38.0 33.0 39.0 24.0 40.0 22 34.170082365012895 38.0 33.0 39.0 23.0 40.0 23 34.58540170276491 38.0 33.0 39.0 25.0 40.0 24 34.06813832884994 38.0 33.0 39.0 23.0 40.0 25 34.36387587010178 38.0 33.0 39.0 25.0 40.0 26 35.04417759782579 38.0 35.0 39.0 27.0 40.0 27 34.980004991818966 38.0 35.0 39.0 27.0 40.0 28 34.768324135444686 38.0 34.0 39.0 27.0 40.0 29 34.66152694195624 38.0 34.0 39.0 26.0 40.0 30 34.35588895976039 38.0 33.0 39.0 25.0 40.0 31 34.77248398458082 38.0 35.0 39.0 27.0 40.0 32 34.38545162095455 38.0 34.0 39.0 25.0 40.0 33 34.591086829917636 38.0 34.0 39.0 27.0 40.0 34 34.478077595052554 38.0 34.0 39.0 26.0 40.0 35 34.26107213178402 38.0 34.0 39.0 25.0 40.0 36 33.49455059763166 36.0 33.0 39.0 25.0 40.0 37 33.182478715438585 36.0 32.0 39.0 24.0 40.0 38 32.9602595745861 36.0 32.0 38.0 23.0 40.0 39 32.61915194542278 36.0 32.0 38.0 19.0 40.0 40 29.255081949027982 35.0 23.0 38.0 7.0 39.0 41 25.813444632407997 31.0 13.0 37.0 4.0 39.0 42 29.97723175906154 34.0 28.0 38.0 4.0 39.0 43 28.33043068304723 33.0 24.0 37.0 4.0 39.0 44 28.511855570037994 33.0 25.0 38.0 4.0 39.0 45 29.65209795058099 34.0 28.0 38.0 4.0 39.0 46 29.3101028869353 35.0 27.0 38.0 4.0 39.0 47 28.895865109958677 34.0 26.0 38.0 4.0 39.0 48 28.97321057156327 34.0 26.0 38.0 4.0 39.0 49 26.522671177791953 33.0 14.0 38.0 4.0 39.0 50 28.62874733076347 34.0 25.0 38.0 4.0 39.0 51 28.833439640589035 35.0 26.0 39.0 4.0 40.0 52 28.611165035081395 35.0 25.0 39.0 4.0 40.0 53 28.190354696469676 34.0 23.0 39.0 4.0 40.0 54 27.32568845503203 33.0 17.0 38.0 4.0 40.0 55 27.62561357774758 34.0 21.0 38.0 4.0 40.0 56 26.55481294545051 33.0 15.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.7847259470100383 1 2 1.68417018611202 1 3 2.3746698523800163 1 4 1.475449451483307 1 5 2.3697310655859454 1 6 4.15525243437547 1 7 2.2845495394768562 1 8 2.1332274826098256 1 9 2.1322475086047348 1 10 2.599679544080388 1 11 -2.5328018583645644 1 12 -13.360256239048386 1 13 -7.508108558222169 1 14 -4.985311264000707 1 15 -5.469627251637096 1 16 -23.284258980245834 1 17 -6.149644635246428 1 18 -14.009352753155074 1 19 -5.47477975664976 1 20 -6.519280851093708 1 21 -7.077185307950067 1 22 -3.133861699006726 1 23 -4.218178362097916 1 24 -4.817295476176518 1 25 -4.067132672731461 1 26 2.8542747579440686 1 27 2.552351246967909 1 28 2.8319530556805717 1 29 2.8060975500043313 1 30 2.835153456912785 1 31 2.920027536472219 1 32 3.408664914540303 1 33 2.5601743633228935 1 34 2.2605773583848148 1 35 2.8395710940626344 1 36 3.4039383168415966 1 37 3.754943711953402 1 38 3.7034912256734316 1 39 3.7336979076665564 1 40 4.48696214990386 1 41 -11.51495407070242 1 42 -11.287645503887514 1 43 -18.092481870340112 1 44 -21.165117848599426 1 45 -14.869960474825861 1 46 -17.91907440864247 1 47 -22.4872470279842 1 48 -16.85830638826694 1 49 -19.105615228596655 1 50 -16.139220035660458 1 51 -13.974433048039115 1 52 -13.297365044504055 1 53 -12.994383659664113 1 54 -12.225171461903852 1 55 -12.748589015797357 1 56 -9.616757336835578 2 1 1.3538352054567255 2 2 2.3682421474985915 2 3 2.8191630511514063 2 4 1.68080267658204 2 5 3.4127323819527717 2 6 4.938051907828736 2 7 2.639091005031922 2 8 2.1481463066554625 2 9 2.2081579956604642 2 10 3.1841464155152224 2 11 2.177597439573127 2 12 -3.8236173623480774 2 13 -2.4300041264538486 2 14 3.3898532818527336 2 15 3.248670247265931 2 16 1.627544442307915 2 17 2.3615444827877994 2 18 -4.873766969916812 2 19 1.0855537229465497 2 20 1.916436568827308 2 21 2.309826539351384 2 22 2.2404252689616797 2 23 2.090290264492694 2 24 0.8891547212785085 2 25 2.8815290209938595 2 26 2.520356372687381 2 27 3.081091922702882 2 28 2.3111105809109347 2 29 2.08385095061864 2 30 2.6692912366407384 2 31 2.7427568036946894 2 32 2.7816355156811525 2 33 2.9164709846480363 2 34 1.8147677927858723 2 35 0.5719098391262634 2 36 0.9581287512426542 2 37 1.0857905746124672 2 38 1.4165232572662347 2 39 1.4748124315805562 2 40 5.528647099443134 2 41 -3.5763845226550295 2 42 -4.98356476672209 2 43 -10.793227811542394 2 44 -14.628478971899117 2 45 -4.823010057976365 2 46 -10.803234127817547 2 47 -18.42669415391663 2 48 -8.476560008275712 2 49 -13.356602503717323 2 50 -7.861027846103635 2 51 -3.7835598580435033 2 52 -3.5615159879002825 2 53 -3.750417007623742 2 54 -2.671858605387836 2 55 -3.347535922335311 2 56 -0.7510267532462827 3 1 1.9598285461892502 3 2 3.009751581460854 3 3 3.336366158809561 3 4 2.218723505290882 3 5 3.6250890227222854 3 6 5.209971996618968 3 7 2.9949918559383235 3 8 3.2750423480908992 3 9 3.0032005409804796 3 10 3.375785335234056 3 11 4.049961479528733 3 12 6.207459219227946 3 13 4.364668459561692 3 14 5.40502161333627 3 15 4.672643610196008 3 16 5.713005041641992 3 17 5.353775337393792 3 18 5.585722486243014 3 19 4.732242587171491 3 20 4.925315592134744 3 21 3.9857421886299633 3 22 3.1224082508336792 3 23 4.120626927459767 3 24 3.279831746805698 3 25 3.521558617738215 3 26 2.470781825887528 3 27 1.9723238871867892 3 28 2.3244291158720856 3 29 2.138974886985636 3 30 3.4580444737846463 3 31 2.1726495154963885 3 32 3.3658012574495544 3 33 2.754428809287326 3 34 2.5487670528672624 3 35 2.98959389388024 3 36 3.8882064426965925 3 37 4.55156933894839 3 38 3.8649139342917636 3 39 4.126310766763684 3 40 8.600049245207096 3 41 8.845739043752666 3 42 5.478884363873547 3 43 -0.4750628096925986 3 44 2.4222054528141648 3 45 5.0278963423196 3 46 3.483114373847272 3 47 -1.9231795405240995 3 48 3.812008249215964 3 49 6.121014958361847 3 50 4.2018652356573725 3 51 4.6744497608984155 3 52 5.349693779025358 3 53 4.628051360855725 3 54 6.2426807952780905 3 55 4.733855124649519 3 56 5.5578892659916015 4 1 1.7810050167774847 4 2 2.469751581460855 4 3 3.130483805868387 4 4 1.9838215445065686 4 5 3.777638042330132 4 6 4.924089643677796 4 7 2.9036193069187206 4 8 3.072297250051683 4 9 2.434573090000086 4 10 3.7173539626850385 4 11 4.012314420705202 4 12 6.343929807463244 4 13 5.165844930149923 4 14 6.003060829022548 4 15 5.417349492548947 4 16 5.288299159289053 4 17 4.979657690334967 4 18 5.349251898007715 4 19 4.874987685210705 4 20 4.810021474487684 4 21 3.533977482747609 4 22 4.269859231225837 4 23 3.4767053588323122 4 24 4.046890570335108 4 25 3.900774304012728 4 26 2.6445073160836046 4 27 2.314676828363261 4 28 3.0714879394015 4 29 3.238190573260148 4 30 3.3760836894709243 4 31 2.5004926527512907 4 32 3.705409100586813 4 33 1.5285464563461488 4 34 2.7777866607103974 4 35 2.6809664428998445 4 36 3.601147619167179 4 37 3.9684320840464267 4 38 4.302560993115293 4 39 4.561604884410745 4 40 7.747892382461998 4 41 10.979464533948743 4 42 7.423982403089234 4 43 9.133564641287794 4 44 8.939068197912203 4 45 7.358876734476468 4 46 8.454879079729626 4 47 7.933683204573942 4 48 6.984949425686551 4 49 9.144152213263808 4 50 6.875198568990708 4 51 7.09719485893763 4 52 5.322634955495943 4 53 6.218639596149838 4 54 7.387386677631035 4 55 6.496992379551482 4 56 6.4316147561876775 5 1 1.6093383501108178 5 2 1.8847515814608542 5 3 2.817983805868387 5 4 1.5471548778399011 5 5 3.0184713756634665 5 6 4.316589643677794 5 7 1.7511193069187172 5 8 1.7872972500516866 5 9 1.7679064233334216 5 10 2.6098539626850368 5 11 3.286481087371868 5 12 5.467263140796579 5 13 4.3891782634832595 5 14 5.398894162355884 5 15 5.18734949254895 5 16 4.969132492622386 5 17 5.497157690334966 5 18 5.096751898007717 5 19 4.324154351877368 5 20 3.710854807821022 5 21 3.469810816080944 5 22 2.499859231225834 5 23 3.29587202549898 5 24 3.968557237001775 5 25 2.7691076373460604 5 26 2.367840649416941 5 27 2.5213434950299245 5 28 1.6798212727348343 5 29 1.9073572399268102 5 30 2.743583689470924 5 31 2.384659319417956 5 32 2.0729091005868128 5 33 1.1527131230128163 5 34 1.3686199940437334 5 35 1.7334664428998465 5 36 2.3661476191671795 5 37 1.7292654173797644 5 38 2.4200609931152925 5 39 3.0699382177440775 5 40 5.7495590491286634 5 41 8.680297867282079 5 42 3.348982403089231 5 43 6.822731307954459 5 44 6.287401531245539 5 45 4.728876734476465 5 46 5.207379079729623 5 47 5.692016537907275 5 48 5.769116092353215 5 49 8.255818879930473 5 50 5.826865235657369 5 51 3.8080281922709602 5 52 4.515134955495945 5 53 4.181972929483173 5 54 4.3615533442977 5 55 2.8061590462181485 5 56 1.9341147561876788 6 1 2.2488311037340054 6 2 3.2923602771130263 6 3 3.956570762390129 6 4 3.0725171966804794 6 5 4.128072824938826 6 6 5.591046165416927 6 7 3.4766627851795846 6 8 3.477514641356038 6 9 3.108486133478344 6 10 3.9495278757285135 6 11 4.62796659461825 6 12 7.057842850941501 6 13 6.8493231910194865 6 14 6.720452133370376 6 15 6.915610362114169 6 16 5.209168724506441 6 17 5.6031359512045285 6 18 6.148382332790327 6 19 5.0732485547759225 6 20 5.1465432136181235 6 21 4.291368787095436 6 22 4.684641839921483 6 23 3.9906184023105737 6 24 4.731238396422064 6 25 4.473817782273592 6 26 3.567116011735777 6 27 3.85467682836326 6 28 3.49322706983628 6 29 3.852103616738404 6 30 4.643040211210057 6 31 3.563101348403464 6 32 4.385409100586813 6 33 3.2876768911287613 6 34 2.4238736172321396 6 35 3.4644447037694093 6 36 4.197669358297617 6 37 4.73017121448121 6 38 4.8086479496370345 6 39 4.774648362671609 6 40 7.96876194767939 6 41 4.050768881774829 6 42 6.702243272654449 6 43 7.8979124673747485 6 44 7.709502980520899 6 45 5.84844195186777 6 46 8.35140081886006 6 47 8.472813639356549 6 48 7.838862469164809 6 49 10.464152213263809 6 50 7.796068134208095 6 51 8.689368771981105 6 52 7.7678523468002965 6 53 8.707335248323755 6 54 9.333473634152774 6 55 8.359601075203653 6 56 8.589006060535507 7 1 1.9843383501108178 7 2 2.5514182481275185 7 3 3.4013171392017227 7 4 2.547154877839901 7 5 3.7684713756634665 7 6 5.0457563103444585 7 7 2.709452640252053 7 8 2.6622972500516866 7 9 3.309573090000086 7 10 3.4431872960183725 7 11 2.848981087371868 7 12 5.488096474129907 7 13 6.035011596816588 7 14 5.482227495689212 7 15 6.12484949254895 7 16 5.239965825955714 7 17 5.517991023668301 7 18 4.909251898007717 7 19 4.386654351877368 7 20 4.7316881411543505 7 21 3.5531441494142726 7 22 3.937359231225834 7 23 3.5042053588323157 7 24 2.864390570335111 7 25 3.0816076373460604 7 26 2.5136739827502694 7 27 2.22967682836326 7 28 2.3673212727348343 7 29 2.84485723992681 7 30 2.618583689470924 7 31 1.9888259860846276 7 32 3.2187424339201485 7 33 1.9027131230128163 7 34 2.5352866607103977 7 35 2.275133109566511 7 36 2.8036476191671795 7 37 2.5209320840464287 7 38 3.1075609931152925 7 39 3.3199382177440775 7 40 5.6870590491286634 7 41 -1.9030354660512572 7 42 3.453149069755895 7 43 5.343564641287795 7 44 4.495734864578871 7 45 3.853876734476465 7 46 5.228212413062959 7 47 4.108683204573939 7 48 4.664949425686551 7 49 6.672485546597141 7 50 5.160198568990705 7 51 3.9330281922709602 7 52 4.848468288829281 7 53 5.327806262816505 7 54 4.778220010964365 7 55 5.097825712884813 7 56 6.267448089521011 8 1 2.1232272389997036 8 2 3.1875293592386313 8 3 3.7415949169795013 8 4 2.8971548778398954 8 5 4.153193597885689 8 6 5.192978532566684 8 7 3.6080637513631615 8 8 3.5456305833850195 8 9 3.3901286455556416 8 10 3.719576184907261 8 11 4.3323144207052025 8 12 6.272818696352129 8 13 6.283622707927698 8 14 6.569727495689218 8 15 6.50401615921562 8 16 5.392743603733493 8 17 5.881879912557189 8 18 6.142585231341052 8 19 5.17276546298848 8 20 4.845577030043245 8 21 4.529533038303164 8 22 4.77208145344806 8 23 4.005594247721206 8 24 5.126890570335114 8 25 4.360774304012722 8 26 3.2067295383058294 8 27 3.810232383918816 8 28 3.704821272734833 8 29 3.6893016843712587 8 30 2.2005281339153697 8 31 1.6027148749735147 8 32 2.62985354503126 8 33 0.8041020119017048 8 34 2.897786660710395 8 35 2.7987442206776265 8 36 2.7300365080560667 8 37 2.508432084046426 8 38 1.7825609931152897 8 39 2.8949382177440732 8 40 3.4301146046842206 8 41 -2.684979910495702 8 42 3.4906490697558965 8 43 5.226897974621128 8 44 4.356845975689982 8 45 3.8788767344764636 8 46 5.272656857507403 8 47 5.3447943156850535 8 48 5.831616092353215 8 49 7.519707768819366 8 50 5.601865235657375 8 51 5.870528192270964 8 52 5.8137460666070595 8 53 7.031972929483171 8 54 8.118497788742147 8 55 7.354770157329259 8 56 8.271614756187677 9 1 1.4662224080818334 9 2 2.661925494504331 9 3 3.304396849346645 9 4 2.007299805376128 9 5 3.5845945640692634 9 6 5.047567904547357 9 7 2.6940540895274125 9 8 2.8905581196169052 9 9 2.543268742174 9 10 2.8408322235545995 9 11 2.888836159835641 9 12 5.753495024854548 9 13 4.9580188431934005 9 14 5.807408655109501 9 15 5.198219057766341 9 16 4.774386115810792 9 17 5.124875081639317 9 18 5.604904071920764 9 19 4.1819442069498365 9 20 4.559586691878991 9 21 2.5739774827476083 9 22 3.597685318182357 9 23 3.012357532745362 9 24 3.383412309465548 9 25 3.0607743040127247 9 26 2.8062464465183865 9 27 1.8329376979284788 9 28 2.1454009828797567 9 29 1.9825383993470993 9 30 2.882170645992666 9 31 1.5196230875339012 9 32 3.1462786658042035 9 33 1.8094160215635426 9 34 1.1630040520147489 9 35 0.5514012255085419 9 36 3.480278053949789 9 37 3.23017121448121 9 38 3.004300123550074 9 39 4.4050831452803045 9 40 5.360066295505476 9 41 5.1159862730791765 9 42 5.897895446567489 9 43 7.158782032592146 9 44 5.622546458781766 9 45 4.326702821432988 9 46 5.025313862338319 9 47 4.5815092915304625 9 48 5.556253773512637 9 49 7.964152213263809 9 50 5.578676829860267 9 51 6.471977467633277 9 52 5.920026259843773 9 53 6.120378726584622 9 54 7.529125808065814 9 55 5.403079336073219 9 56 5.5237886692311555 10 1 1.3771954929679566 10 2 1.8668944386037083 10 3 2.535245710630292 10 4 1.1066786873637113 10 5 2.6881142328063206 10 6 3.742184881773035 10 7 1.6826669259663376 10 8 1.3646782024326356 10 9 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115 29 1.421433816503388 115 30 1.6371647705520047 115 31 1.6183304905891305 115 32 1.5475712627489742 115 33 1.913411321211015 115 34 1.5842731471968818 115 35 0.9344799564133623 115 36 2.6195259975455585 115 37 2.681405057019404 115 38 2.7933718039261066 115 39 3.523767046572903 115 40 6.937622112191725 115 41 5.402707777191985 115 42 1.088847267954094 115 43 -3.149678601955447 115 44 -5.637148018304014 115 45 2.2626605182602475 115 46 -3.051066866216324 115 47 -2.6041546332638994 115 48 -2.808023547286421 115 49 -10.87368562457403 115 50 -4.167504133711997 115 51 -2.381724059981291 115 52 -2.6757434228824337 115 53 -3.8202793227690783 115 54 -7.370991700747346 115 55 -3.3148995123404106 115 56 -1.5608176762447528 116 1 0.5914812072536719 116 2 1.7716563433656134 116 3 2.320959996344577 116 4 1.6781072587922807 116 5 2.664304708996795 116 6 4.075518215106364 116 7 2.158857402156812 116 8 2.078963916718351 116 9 2.0060016614286553 116 10 2.7259253912564674 116 11 -0.4486379602471757 116 12 -14.116070192536757 116 13 -4.956059831754839 116 14 -1.425510599548879 116 15 -2.110269555070097 116 16 -4.0278913169014245 116 17 -1.797485166807892 116 18 -7.471700482944662 116 19 -1.571678981455964 116 20 -2.119502335036124 116 21 1.859691768461893 116 22 2.6308116121782135 116 23 2.2452767874037463 116 24 2.5459381893827313 116 25 2.679821923060345 116 26 2.617840649416941 116 27 2.73562920931564 116 28 2.400059367972929 116 29 2.7020000970696714 116 30 3.3417979751852087 116 31 1.6138259860846276 116 32 2.3615995767772873 116 33 2.3818797896794877 116 34 2.4787390416627773 116 35 1.992395014328416 116 36 3.5268619048814642 116 37 3.2560511316654797 116 38 3.3158943264486282 116 39 3.8377953606012163 116 40 7.3269400015096195 116 41 5.040416914901122 116 42 -0.2522080731012437 116 43 -8.19214964442649 116 44 -7.9923603735163695 116 45 -1.0449327893330604 116 46 -4.057501872651329 116 47 -2.504412033521298 116 48 -4.358860098122975 116 49 -5.392990643879049 116 50 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2.6267884109316384 117 33 1.8416499046220167 117 34 2.7184763158828105 117 35 1.007173339451569 117 36 2.8618372743395923 117 37 3.1222251874947062 117 38 3.6032506482877054 117 39 3.8098807464797098 117 40 6.656168244530967 117 41 5.297395568431501 117 42 1.7757065410202664 117 43 -9.940918117332895 117 44 -7.041621457260209 117 45 2.823704320683362 117 46 -3.036155403028996 117 47 -3.3180409333570964 117 48 -2.9384988501755167 117 49 -7.759985717770675 117 50 -6.361353155147228 117 51 -4.5662534169244395 117 52 -4.297365044504055 117 53 -4.556532817643262 117 54 -7.8546822878862095 117 55 -3.4574903790692098 117 56 -2.5421783472605988 118 1 0.20100501677747928 118 2 1.1764182481275185 118 3 1.9304838058683842 118 4 1.1971548778398997 118 5 2.1976380423301336 118 6 3.5374229770111256 118 7 1.6636193069187186 118 8 1.3456305833850166 118 9 1.4012397566667545 118 10 2.397353962685038 118 11 -2.001018912628126 118 12 -6.316070192536756 118 13 -8.327488403183409 118 14 -5.930272504310786 118 15 -6.395983840784382 118 16 -12.485034174044284 118 17 -14.307008976331701 118 18 -13.75741476865895 118 19 -5.371678981455961 118 20 -6.043311858845648 118 21 -0.5593558505857246 118 22 0.5831925645591696 118 23 -0.08329464116768293 118 24 0.29355723700177805 118 25 1.227440970679389 118 26 2.0178406494169394 118 27 1.6546768283632574 118 28 1.1048212727348314 118 29 1.5115239065934816 118 30 1.1894170228042569 118 31 -0.15284068058203815 118 32 -2.9812575660798544 118 33 0.0818797896794834 118 34 -0.2688800059562695 118 35 0.5542997762331794 118 36 0.6078142858338467 118 37 1.0417654173797644 118 38 0.21589432644862683 118 39 0.4616048844107432 118 40 5.141225715795329 118 41 0.25946453394874425 118 42 -6.07601759691077 118 43 -11.739768692045539 118 44 -9.65426513542113 118 45 -5.821123265523536 118 46 -14.03845425360371 118 47 -14.432983462092727 118 48 -15.268383907646783 118 49 -10.602514453402858 118 50 -12.631468097675961 118 51 -8.296138474395704 118 52 -8.964031711170723 118 53 -9.768027070516826 118 54 -9.1259466557023 118 55 -8.589674287115187 118 56 -6.8617185771456555 119 1 -1.0080858923134244 119 2 0.07035764206691653 119 3 0.6910898664744494 119 4 -0.005875425190403405 119 5 0.9370319817240684 119 6 2.625301764889919 119 7 0.07877082207023278 119 8 -0.07255123479679781 119 9 0.49517915060614826 119 10 0.5700812354123101 119 11 -5.491928003537222 119 12 -9.313039889506452 119 13 -8.96688234257735 119 14 -10.3454240194623 119 15 -8.714165658966202 119 16 -14.354731143741255 119 17 -15.394887764210488 119 18 -16.408929920174103 119 19 -7.920163829940812 119 20 -9.582705798239587 119 21 -0.21390130513118066 119 22 0.6589501403167404 119 23 0.7106447527717066 119 24 0.7753754188199622 119 25 0.833501576739998 119 26 0.45117398275026943 119 27 0.9152828889693225 119 28 0.7290636969772564 119 29 0.08728148235105238 119 30 1.5863867197739552 119 31 -1.007386135127497 119 32 -5.826712111534398 119 33 -0.0726656648659727 119 34 -0.9931224301986958 119 35 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-17.055726925049647 120 18 -15.590748101992283 120 19 -8.78962769940468 120 20 -14.42536314089693 120 21 -6.695253286483162 120 22 -5.019371538004936 120 23 -4.067910025783068 120 24 -8.380801737357196 120 25 -3.8623026190641987 120 26 0.7845073160836051 120 27 0.4700614437478734 120 28 0.9714879394014986 120 29 0.8704982655678393 120 30 1.056083689470924 120 31 -0.9887381164794746 120 32 -16.153052437874727 120 33 -1.451453543653848 120 34 -1.4406748777511424 120 35 -1.5790335571001535 120 36 0.7026860807056394 120 37 1.2084320840464287 120 38 1.0979456084999057 120 39 0.7077587305645885 120 40 2.9002000747696925 120 41 0.8517722262564362 120 42 -3.6990945199876926 120 43 -11.02181997409682 120 44 -5.80554718670318 120 45 -8.290354034754305 120 46 -13.75896707411653 120 47 -14.881701410810676 120 48 -10.911973651236526 120 49 -17.651232402120804 120 50 -12.58018604639391 120 51 -11.001266679523912 120 52 -11.566595813734825 120 53 -11.247514250004006 120 54 -15.069536399292044 120 55 -12.999930697371596 120 56 -10.359154474581551 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 704.0 5 44.0 6 47.0 7 48.0 8 37.0 9 33.0 10 38.0 11 30.0 12 41.0 13 45.0 14 59.0 15 97.0 16 114.0 17 159.0 18 182.0 19 251.0 20 280.0 21 380.0 22 503.0 23 607.0 24 686.0 25 708.0 26 856.0 27 810.0 28 1017.0 29 1205.0 30 1282.0 31 1624.0 32 1820.0 33 2133.0 34 2529.0 35 3067.0 36 3894.0 37 4513.0 38 3942.0 39 2274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 98.2584098283369 1.3672037494106881 0.061011120663357274 0.3133753015890624 8 98.29446185418342 1.2590476718711001 0.06933081893563327 0.377159655009845 9 97.69821681133698 1.5724229734601625 0.24681771541085443 0.4825424997920076 10 47.796666574225576 30.09512188357969 14.897806372888878 7.21040516930586 11 29.041293435758064 40.919049335810755 14.811836157408692 15.22782107102249 12 32.241604037826896 14.723092709171082 19.750963698383206 33.28433955461882 13 21.391323643625874 15.383335182514147 26.001331410185287 37.224009763674694 14 20.909721259187354 20.072112051033145 34.416863125780054 24.601303563999448 15 30.832939607014815 18.01228426113782 25.057669325477335 26.09710680637003 16 32.708050059660906 20.07936287704304 23.0901573382912 24.122429725004856 17 25.114395851243792 20.03937990515544 30.65254166782218 24.193682575778585 18 22.40619019885182 22.214826524669274 30.421277421859834 24.957705854619075 19 32.50437147853118 23.264592411668378 20.308640262011156 23.92239584778928 20 23.558479775686404 31.99244884928236 21.354210044140917 23.094861330890314 21 24.709503868659695 20.571840594581104 29.662497573421337 25.05615796333786 22 24.48209878255082 21.00723813749688 21.686680163066086 32.823982916886216 23 24.35730330846668 22.080479214620482 22.2025014559472 31.35971602096564 24 22.751601541917413 22.33838986106104 31.215507917579522 23.694500679442026 25 31.281027262390104 22.952547355575895 23.21879246748204 22.54763291455196 26 22.54700759886849 21.72610793721227 25.42847634366853 30.29840812025071 27 22.092377727197025 19.541442291678234 27.03047798811969 31.33570199300505 28 21.97912039093736 27.101843625055533 29.484118169702356 21.434917814304754 29 29.492052043165867 19.14722445695897 30.63500429994174 20.72571919993342 30 20.380540300660122 18.738558828424033 40.60021079491874 20.280690075997114 31 20.673010236635502 27.694398979110606 32.760007767636694 18.872583016617195 32 30.180142670774696 18.16137896577567 33.43880978155273 18.21966858189691 33 21.04049775012499 17.576801288817286 35.78967835120271 25.59302260985501 34 18.37402885682575 26.75083240843507 38.11875693673696 16.75638179800222 35 18.801331853496116 18.873473917869035 47.11154273029967 15.213651498335185 36 19.5305997891583 26.20262997281252 38.09576652055707 16.171003717472118 37 18.63347133846453 18.536219400372335 45.811220095029036 17.019089166134098 38 27.339730218328466 18.815185648727574 38.12821582533722 15.71686830760673 39 19.737207622645702 17.97322073448525 37.690982832379575 24.59858881048947 40 14.778943842804509 15.183655241710396 39.10070336050017 30.936697554984928 41 12.941735629613063 14.901588011630507 37.2316036680832 34.925072690673225 42 24.225086101544274 17.964670592156427 39.6594822797467 18.150761026552605 43 15.638637943015985 15.335649756775538 46.045865184155666 22.979847116052817 44 15.274449707439397 15.491780440234049 38.17776539426024 31.056004458066315 45 17.600133418572977 17.81137949245351 47.36359340689885 17.22489368207466 46 16.554119772127276 19.024593580658607 38.96345699597054 25.457829651243575 47 15.911550092944537 25.538939599922315 39.71090086840718 18.838609438725967 48 17.743145262105177 26.47998444314804 39.17548684612607 16.60138344862072 49 14.09506296667781 21.116126156246516 34.77655187785579 30.01225899921988 50 23.4447900466563 19.14574538991335 39.76060875361031 17.64885580982004 51 17.548056861490526 27.868806854535844 38.2841405402398 16.29899574373383 52 17.671887148728203 19.801732755748084 45.36821059646785 17.15816949905587 53 16.95141261771814 20.326693891157596 44.4620385032086 18.25985498791566 54 16.46793963145168 25.90399955529615 35.77920453597932 21.848856277272855 55 16.13235089670513 27.582371750312806 38.87946614764354 17.405811205338523 56 24.155367859524336 21.593687486108024 36.14414314292065 18.10680151144699 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 2.5 4 7.5 5 10.0 6 11.0 7 12.0 8 13.5 9 15.0 10 23.5 11 32.0 12 72.0 13 139.5 14 167.0 15 213.0 16 259.0 17 331.5 18 404.0 19 439.5 20 475.0 21 514.0 22 712.5 23 872.0 24 1038.0 25 1204.0 26 1303.0 27 1402.0 28 1451.5 29 1523.5 30 1546.0 31 1389.0 32 1232.0 33 1011.5 34 791.0 35 690.5 36 590.0 37 572.0 38 517.0 39 480.0 40 522.5 41 565.0 42 663.5 43 762.0 44 1308.5 45 1855.0 46 1847.0 47 1507.0 48 1175.0 49 1138.5 50 1102.0 51 1241.5 52 1381.0 53 1417.0 54 1488.5 55 1524.0 56 1543.5 57 1563.0 58 1719.5 59 1876.0 60 1926.0 61 1976.0 62 1825.5 63 1579.0 64 1483.0 65 1314.0 66 1145.0 67 985.5 68 826.0 69 717.0 70 608.0 71 511.0 72 354.0 73 294.0 74 233.5 75 173.0 76 128.0 77 83.0 78 64.0 79 32.5 80 20.0 81 12.5 82 5.0 83 3.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.019412629301977312 14 0.011092931029701323 15 0.21631215507917578 16 0.061011120663357274 17 0.0 18 0.005546465514850662 19 0.08319698272275992 20 0.10538284478216256 21 0.0 22 0.0 23 0.0 24 0.0 25 0.005546465514850662 26 0.002773232757425331 27 0.0915166809950359 28 0.11924900856928923 29 0.03327879308910397 30 0.013866163787126655 31 0.03327879308910397 32 0.08874344823761059 33 0.15807426717324385 34 0.05269142239108128 35 0.05269142239108128 36 0.0360520258465293 37 0.19412629301977316 38 0.2884162067722344 39 0.16916719820294518 40 0.6406167669652514 41 0.8070107324107713 42 0.1525278016583932 43 0.23295155162372777 44 0.46867633600488084 45 0.2274050861088771 46 0.20521922404947449 47 0.04437172411880529 48 0.1719404309603705 49 0.4631298704900302 50 0.14143487062869187 51 0.310602068831637 52 0.13034193959899054 53 0.1719404309603705 54 0.22185862059402647 55 0.2606838791979811 56 0.18580659474749717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 36059.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.84614104661804 #Duplication Level Percentage of deduplicated Percentage of total 1 86.55961709190566 62.18974458526304 2 7.681321650519164 11.037466374552816 3 2.5359941328598445 5.466041764885326 4 1.150268267263674 3.305693446850994 5 0.640753464314664 2.3017831886630242 6 0.3821361022117574 1.6473002579106464 7 0.2740572046165129 1.3782966804403893 8 0.18141814953487476 1.0427355167919243 9 0.10421893696684294 0.6738955600543554 >10 0.4593353147797892 5.510413489004132 >50 0.015439842513606363 0.71272081865831 >100 0.01157988188520477 2.0605119387670205 >500 0.0038599606284015907 2.673396378158019 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 964 2.673396378158019 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 286 0.7931445686236447 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 256 0.7099475859008847 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 201 0.5574197842424915 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 83 0.23017831886630244 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 62 0.1719404309603705 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 60 0.16639396544551985 No Hit CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52 0.1442081033861172 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 50 0.13866163787126654 No Hit CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG 45 0.12479547408413988 No Hit CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 43 0.11924900856928923 No Hit CAACAAGGGGAGTTCTTTGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTG 39 0.10815607753958789 No Hit CAACAAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG 39 0.10815607753958789 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.002773232757425331 0.0 0.0 0.0 0.0 7 0.002773232757425331 0.0 0.0 0.0 0.0 8 0.002773232757425331 0.0 0.0 0.0 0.0 9 0.002773232757425331 0.0 0.0 0.0 0.0 10 0.013866163787126653 0.0 0.0 0.0 0.0 11 0.027732327574253306 0.0 0.0 0.0 0.0 12 0.03327879308910397 0.0 0.0 0.0 0.0 13 0.03882525860395463 0.0 0.0 0.0 0.0 14 0.04159849136137996 0.0 0.0 0.0 0.0 15 0.047144956876230625 0.0 0.0 0.0 0.0 16 0.05269142239108128 0.0 0.0 0.0 0.0 17 0.05546465514850661 0.0 0.0 0.0 0.0 18 0.0637843534207826 0.0 0.0 0.0 0.0 19 0.06655758617820794 0.0 0.0 0.0 0.0 20 0.08042374996533459 0.0 0.0 0.0 0.0 21 0.08042374996533459 0.0 0.0 0.0 0.0 22 0.08319698272275992 0.0 0.0 0.0 0.0 23 0.08874344823761059 0.0 0.0 0.0 0.0 24 0.09151668099503592 0.0 0.0 0.0 0.0 25 0.10538284478216256 0.0 0.0 0.0 0.0 26 0.11092931029701322 0.0 0.0 0.0 0.0 27 0.13034193959899054 0.0 0.0 0.0 0.0 28 0.13866163787126654 0.0 0.0 0.0 0.0 29 0.1442081033861172 0.0 0.0 0.0 0.0 30 0.1442081033861172 0.0 0.0 0.0 0.0 31 0.1442081033861172 0.0 0.0 0.0 0.0 32 0.1442081033861172 0.0 0.0 0.0 0.0 33 0.14975456890096786 0.0 0.0 0.0 0.0 34 0.14975456890096786 0.0 0.0 0.0 0.0 35 0.14975456890096786 0.0 0.0 0.0 0.0 36 0.1525278016583932 0.0 0.0 0.0 0.0 37 0.15530103441581852 0.0 0.0 0.0 0.0 38 0.15530103441581852 0.0 0.0 0.0 0.0 39 0.15530103441581852 0.0 0.0 0.0 0.0 40 0.15530103441581852 0.0 0.0 0.0 0.0 41 0.16084749993066919 0.0 0.0 0.0 0.0 42 0.16916719820294518 0.0 0.0 0.0 0.0 43 0.16916719820294518 0.0 0.0 0.0 0.0 44 0.17471366371779584 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGAAC 15 0.008126377 50.366947 38 AGAACCT 15 0.008126377 50.366947 40 CGTCTCA 55 0.0 50.366947 41 TATGCTC 45 1.2369128E-10 50.22626 35 TGCTCGT 45 1.2369128E-10 50.22626 37 ATGCTCG 45 1.2369128E-10 50.22626 36 CTCTGGC 15 0.008216911 50.226257 37 CGGAGAA 15 0.008216911 50.226257 37 TATGCCT 15 0.008216911 50.226257 35 TTTTCCA 15 0.008216911 50.226257 36 ATGCCTG 15 0.008216911 50.226257 36 ATACACA 15 0.008216911 50.226257 36 TATGCTT 195 0.0 50.226254 35 ATATGCT 255 0.0 50.156208 34 ATATGCC 45 1.2551027E-10 50.156208 34 CAAGATT 20 4.0945332E-4 50.086353 47 GTGCAAT 20 4.0945332E-4 50.086353 49 AACTCTG 15 0.008308194 50.086353 47 ACTTTCG 15 0.008308194 50.086353 45 AAGATTC 15 0.008308194 50.086353 48 >>END_MODULE