FastQCFastQC Report
Fri 17 Jun 2016
SRR1526272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21870
Sequences flagged as poor quality0
Sequence length56
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1830.8367626886145405No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1710.7818930041152263No Hit
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG900.411522633744856No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG860.39323273891175126No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG770.3520804755372657No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG640.2926383173296754No Hit
CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG630.2880658436213992No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG500.2286236854138089No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC430.19661636945587563No Hit
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG400.18289894833104708No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC390.17832647462277093No Hit
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG360.1646090534979424No Hit
CTCGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.15089163237311387No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC330.15089163237311387No Hit
CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG230.10516689529035209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT251.8196035E-550.8849839
CCCACAG402.1191227E-950.8849840
ACCCACA402.1191227E-950.8849839
TACCCAC402.1191227E-950.8849838
ACGCCCA354.32683E-850.88497539
TACGCCC354.32683E-850.88497538
TCCAAGT150.00776120650.88497538
CCAAGTG150.00776120650.88497539
CCGACGG150.00776120650.88497539
CAAGTGG150.00776120650.88497540
TGCCGAC150.00783373250.7658137
GTACCCA309.0039794E-750.7658137
GACGGGC150.00783373250.7658141
CTCCAAG150.00783373250.7658137
GTACGCC354.4083208E-850.7658137
TTTTCCT251.8972021E-550.52913736
ACTCCAA150.00798029650.52913736
GGTACCC354.5749402E-850.52913736
AAGAGCC203.9299903E-450.41162535
CGCTGAC150.00812889150.29466247