##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526272_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21870 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.609922267946956 39.0 38.0 40.0 33.0 40.0 2 37.04298125285779 39.0 38.0 40.0 31.0 40.0 3 37.02729766803841 39.0 38.0 40.0 31.0 40.0 4 36.262962962962966 38.0 35.0 39.0 29.0 40.0 5 36.70310928212163 39.0 37.0 40.0 30.0 40.0 6 34.853955189757656 39.0 35.0 40.0 19.0 40.0 7 36.83557384545039 39.0 36.0 39.0 31.0 40.0 8 36.90777320530407 39.0 36.0 40.0 31.0 40.0 9 36.9307727480567 39.0 37.0 40.0 31.0 40.0 10 36.30274348422497 39.0 36.0 39.0 29.0 40.0 11 35.79538180155464 38.0 35.0 39.0 29.0 40.0 12 33.6506630086877 38.0 31.0 39.0 20.0 40.0 13 33.451074531321446 38.0 31.0 39.0 20.0 40.0 14 33.41979881115684 38.0 31.0 39.0 19.0 40.0 15 33.54481024234111 38.0 31.0 39.0 20.0 40.0 16 33.75258344764518 38.0 32.0 39.0 21.0 40.0 17 33.89172382258802 38.0 32.0 39.0 22.0 40.0 18 33.37864654778235 37.0 31.0 39.0 20.0 40.0 19 34.23616826703246 38.0 32.0 39.0 24.0 40.0 20 34.19419295839049 38.0 32.0 39.0 24.0 40.0 21 35.13086419753086 38.0 34.0 39.0 27.0 40.0 22 34.75637860082305 38.0 33.0 39.0 25.0 40.0 23 35.06625514403292 38.0 34.0 39.0 27.0 40.0 24 34.58545953360768 38.0 33.0 39.0 24.0 40.0 25 34.954366712391405 38.0 34.0 39.0 26.0 40.0 26 35.36634659350709 38.0 35.0 39.0 28.0 40.0 27 35.26470050297211 38.0 35.0 39.0 27.0 40.0 28 35.10516689529035 38.0 35.0 39.0 27.0 40.0 29 35.006310013717425 38.0 35.0 39.0 27.0 40.0 30 34.65505258344764 38.0 33.0 39.0 26.0 40.0 31 35.10727023319616 38.0 35.0 39.0 28.0 40.0 32 34.67466849565615 38.0 34.0 39.0 26.0 40.0 33 34.893278463648834 38.0 34.0 39.0 27.0 40.0 34 34.802880658436216 38.0 34.0 39.0 27.0 40.0 35 34.72533150434385 38.0 34.0 39.0 27.0 40.0 36 34.15176040237769 37.0 33.0 39.0 27.0 40.0 37 33.87658893461363 36.0 33.0 38.0 26.0 40.0 38 33.22770919067215 36.0 32.0 38.0 24.0 40.0 39 33.281207133058984 36.0 32.0 38.0 23.0 40.0 40 29.898765432098767 35.0 25.0 38.0 9.0 39.0 41 26.406538637402836 31.0 15.0 38.0 5.0 39.0 42 30.57201646090535 34.0 29.0 38.0 10.0 39.0 43 29.099542752629173 33.0 26.0 38.0 5.0 39.0 44 29.011659807956104 33.0 26.0 38.0 4.0 39.0 45 30.167672610882487 34.0 28.0 38.0 4.0 39.0 46 29.83525377229081 35.0 27.0 38.0 4.0 40.0 47 29.264151806127114 34.0 26.0 38.0 4.0 39.0 48 29.45080018289895 34.0 27.0 38.0 4.0 39.0 49 26.89519890260631 33.0 15.0 38.0 4.0 39.0 50 29.03090992226795 34.0 26.0 38.0 4.0 39.0 51 29.214677640603565 35.0 27.0 38.0 4.0 40.0 52 28.96909007773205 35.0 26.0 38.0 4.0 40.0 53 28.62071330589849 34.0 25.0 38.0 4.0 40.0 54 27.440283493369915 33.0 18.0 38.0 4.0 40.0 55 27.96172839506173 34.0 23.0 38.0 4.0 40.0 56 27.346136259716506 34.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.271413693854285 1 2 1.965324914762725 1 3 1.9503133164828697 1 4 2.8239315847870827 1 5 2.449968537597776 1 6 3.458358612995653 1 7 0.6490835135102344 1 8 0.7757424524140859 1 9 0.8076593684136668 1 10 1.4313380445350958 1 11 -2.86340896661639 1 12 -12.689790038093438 1 13 -10.145663351802739 1 14 -5.931198056272187 1 15 -7.991116135222452 1 16 -24.372710892042498 1 17 -5.990070610799588 1 18 -13.058043368159808 1 19 -6.952493276859197 1 20 -6.654004101457804 1 21 -3.3740747750290723 1 22 -1.4965092554597774 1 23 -2.5755346771310457 1 24 -2.8026112953524027 1 25 -2.4161927918148116 1 26 0.6172529077225235 1 27 1.544092905000852 1 28 1.3293075139679544 1 29 1.5122248175376711 1 30 1.6173630423360166 1 31 1.225027099239007 1 32 1.210609573268414 1 33 0.45309956407881913 1 34 1.5335036657226908 1 35 1.7433433642606104 1 36 2.4823169194239014 1 37 1.9778405296128057 1 38 2.2536330158841835 1 39 3.5905401454532537 1 40 6.319441303678765 1 41 -6.745611973166028 1 42 -6.390011180462988 1 43 -18.2657812961756 1 44 -20.785968273308853 1 45 -11.769709479670517 1 46 -15.447528232558273 1 47 -21.292267218457113 1 48 -14.47810348917695 1 49 -18.715924018905525 1 50 -13.11532672726998 1 51 -8.761045813178175 1 52 -8.671477578415743 1 53 -8.203279193808473 1 54 -8.36614917879238 1 55 -7.877203855758758 1 56 -3.9002956917544793 2 1 2.33141369385428 2 2 3.02532491476272 2 3 2.923646649816206 2 4 3.3239315847870827 2 5 3.276635204264444 2 6 5.678358612995652 2 7 3.0624168468435684 2 8 3.015742452414088 2 9 2.8809927017469974 2 10 3.3046713778684307 2 11 2.2499243667169466 2 12 -2.5031233714267707 2 13 -0.5723300184694047 2 14 4.322135277061143 2 15 4.315550531444217 2 16 3.667289107957501 2 17 3.8099293892004127 2 18 0.6619566318401908 2 19 2.940840056474137 2 20 3.759329231875526 2 21 3.5392585583042617 2 22 2.663490744540219 2 23 3.0044653228689526 2 24 1.9840553713142626 2 25 2.823807208185187 2 26 3.0039195743891938 2 27 3.7774262383341792 2 28 3.1493075139679547 2 29 3.685558150871003 2 30 2.8106963756693517 2 31 2.185027099239001 2 32 2.7439429066017453 2 33 2.7930995640788225 2 34 1.4268369990560217 2 35 2.8700100309272756 2 36 1.9223169194238992 2 37 2.4845071962794734 2 38 1.720299682550852 2 39 2.450540145453253 2 40 6.052774637012099 2 41 -6.645611973166027 2 42 -4.390011180462988 2 43 -14.492447962842268 2 44 -12.47263493997552 2 45 -4.783042813003853 2 46 -9.907528232558274 2 47 -17.51226721845711 2 48 -8.25810348917695 2 49 -11.862590685572192 2 50 -6.268660060603313 2 51 -0.10104581317817463 2 52 -0.18481091174907505 2 53 -0.1432791938084712 2 54 1.587184154540953 2 55 1.0561294775745758 2 56 5.253037641578857 3 1 2.2906729531135426 3 2 3.0956952851330968 3 3 3.1236466498162088 3 4 3.4017093625648585 3 5 3.3951537227829576 3 6 5.319099353736391 3 7 2.5587131431398618 3 8 2.297223933895566 3 9 2.884696405450704 3 10 2.434301007498057 3 11 2.868442885235467 3 12 5.7265062582028605 3 13 4.235077388938002 3 14 4.936950091875957 3 15 4.478513494407174 3 16 5.489511330179724 3 17 5.469188648459671 3 18 5.587882557766115 3 19 4.622321537955621 3 20 4.825995898542196 3 21 3.8540733731190713 3 22 4.152379633429106 3 23 4.297057915461551 3 24 4.872944260203155 3 25 3.527510911888889 3 26 2.915030685500305 3 27 4.158907719815666 3 28 3.8196778843383257 3 29 2.885558150871006 3 30 3.1625482275211994 3 31 3.570212284424187 3 32 2.380979943638785 3 33 3.3597662307454854 3 34 2.4120221842412093 3 35 3.8885285494457946 3 36 4.151946549053527 3 37 4.632655344427626 3 38 4.083262645513813 3 39 4.88387347878659 3 40 5.393515377752841 3 41 7.069202841648789 3 42 3.9062851158333096 3 43 -5.11467018506449 3 44 2.2644020970615166 3 45 2.6021423721813335 3 46 1.9109902859602457 3 47 -2.212267218457111 3 48 2.9011557700823083 3 49 4.048520425538918 3 50 1.6239325319892792 3 51 1.3100652979329368 3 52 1.337411310473147 3 53 2.6826467321174547 3 54 3.1760730434298416 3 55 2.9672405886856836 3 56 0.07155616009737287 4 1 1.531413693854283 4 2 1.6253249147627216 4 3 1.990313316482876 4 4 1.3905982514537456 4 5 1.909968537597777 4 6 3.845025279662316 4 7 1.5624168468435684 4 8 2.015742452414088 4 9 1.9809927017469988 4 10 0.20467137786842926 4 11 3.216591033383615 4 12 4.996876628573233 4 13 4.39433664819726 4 14 4.888801943727813 4 15 4.582217198110882 4 16 5.500622441290837 4 17 4.343262722533744 4 18 4.361956631840194 4 19 3.8408400564741356 4 20 4.625995898542193 4 21 2.505925224970923 4 22 2.663490744540219 4 23 2.6377986562022855 4 24 3.3173887046475983 4 25 2.5238072081851897 4 26 2.6372529077225266 4 27 2.0107595716675135 4 28 2.515974180634622 4 29 3.1188914842043403 4 30 1.6440297090026803 4 31 1.3850270992390037 4 32 2.6106095732684125 4 33 0.9597662307454868 4 34 1.593503665722693 4 35 -0.1966566357393873 4 36 2.422316919423899 4 37 2.017840529612812 4 38 2.853633015884185 4 39 2.1172068121199246 4 40 4.119441303678762 4 41 7.787721360167303 4 42 2.27665548620368 4 43 3.9408853704910634 4 44 4.627365060024481 4 45 2.550290520329483 4 46 3.559138434108391 4 47 2.5210661148762235 4 48 2.508563177489716 4 49 5.5374093144278085 4 50 0.7980066060633533 4 51 -0.8677124798448403 4 52 0.9485224215842578 4 53 -2.2766125271418076 4 54 1.0205174878742866 4 55 -0.3772038557587578 4 56 -0.013629025087812607 5 1 2.492952155392743 5 2 3.225324914762723 5 3 3.290313316482873 5 4 3.66239312324862 5 5 3.2868916145208544 5 6 5.693743228380264 5 7 3.3906219750486954 5 8 3.0080501447217785 5 9 3.2322747530290528 5 10 3.432876506073555 5 11 3.747360264152846 5 12 6.660979192675796 5 13 6.302028955889568 5 14 6.673417328343199 5 15 7.002730018623701 5 16 5.959596800265196 5 17 6.017621696892718 5 18 5.546572016455578 5 19 5.422891338525417 5 20 4.441380513926809 5 21 3.667463686509386 5 22 4.801952283001761 5 23 4.012157630561262 5 24 5.009696396955292 5 25 4.708422592800574 5 26 3.406483676953293 5 27 4.036400597308543 5 28 3.6800767447371854 5 29 3.398378663691517 5 30 3.3235168884898627 5 31 3.1927194069313103 5 32 2.6644557271145644 5 33 2.5007918717711277 5 34 4.0473498195688435 5 35 2.7418049027221514 5 36 3.291547688654667 5 37 4.394763606535889 5 38 4.338248400499573 5 39 5.742847837760948 5 40 9.242518226755685 5 41 11.869772642218585 5 42 7.686911896460092 5 43 9.120372549978246 5 44 9.08633941899884 5 45 7.673367443406402 5 46 8.430933305903267 5 47 7.556963550773656 5 48 8.718819587746129 5 49 11.680999058017555 5 50 8.86467327273002 5 51 9.660492648360286 5 52 8.333137806199645 5 53 9.295182344653067 5 54 9.5307738981307 5 55 10.292026913472007 5 56 10.835088923630135 6 1 1.4107240386818702 6 2 2.5184283630385877 6 3 2.859278833724254 6 4 2.175655722718112 6 5 2.5892788824253614 6 6 4.888703440581857 6 7 2.411842134199894 6 8 1.8847079696554658 6 9 1.9166248856550467 6 10 2.092027699707508 6 11 3.3269358609698187 6 12 5.77503754811346 6 13 5.770198717162778 6 14 6.099146771314018 6 15 6.090263175122374 6 16 5.0948753148540575 6 17 3.6582052512693792 6 18 4.9033359421850164 6 19 4.428196378313217 6 20 4.205306243369783 6 21 3.499028673246791 6 22 2.8152148824712526 6 23 2.945844633213781 6 24 3.0415266356820823 6 25 3.1341520357713932 6 26 2.6889770456535587 6 27 2.6544377325870556 6 28 2.4148247553472615 6 29 2.897052403744567 6 30 3.253225111301532 6 31 2.488475375101075 6 32 2.0450923318891014 6 33 1.9344788744236467 6 34 1.0314347002054518 6 35 2.875757157364056 6 36 1.3499031263204557 6 37 0.541978460647293 6 38 1.6260468089876312 6 39 2.240195317867048 6 40 5.836682682989107 6 41 1.8750776820063848 6 42 3.5410233022956312 6 43 6.015598014169225 6 44 5.859548968070456 6 45 3.9571870720536175 6 46 3.495920043303798 6 47 3.3049741608532344 6 48 3.8143103039264936 6 49 6.190282877646197 6 50 4.9853629279024325 6 51 4.647230048890794 6 52 2.620936214687706 6 53 3.7567208061915274 6 54 4.746954269483481 6 55 1.3503823511377924 6 56 1.805911204797244 7 1 1.755551624888767 7 2 2.10463525959031 7 3 2.4454857302759763 7 4 2.1066902054767382 7 5 2.4513478479426 7 6 4.750772406099095 7 7 2.411842134199894 7 8 2.1260872800002915 7 9 2.226969713241253 7 10 1.3678897686730238 7 11 2.878659998900851 7 12 5.016416858458285 7 13 4.873646993024849 7 14 4.961215736831257 7 15 5.297159726846516 7 16 5.508668418302328 7 17 5.037515596096966 7 18 5.1791980111505325 7 19 3.8419894817614946 7 20 4.377720036473228 7 21 3.257649362901958 7 22 2.8152148824712526 7 23 2.0837756676965427 7 24 2.5932507736131143 7 25 3.306565828874845 7 26 1.0682873904811458 7 27 1.3440929050008492 7 28 1.725169582933468 7 29 0.9315351623652575 7 30 1.5635699388877384 7 31 0.5574408923424485 7 32 3.2175061249925463 7 33 1.1758581847684795 7 34 -0.17546185151869054 7 35 2.5654123297778497 7 36 -0.06388997712782185 7 37 0.6799094951300475 7 38 1.7294950848497024 7 39 -0.4494598545467454 7 40 3.767717165747733 7 41 -0.469749904200512 7 42 1.7134370953990796 7 43 2.5673221521002603 7 44 3.100928278415285 7 45 1.6123594858467243 7 46 2.185575215717588 7 47 1.7877327815428892 7 48 2.4005172004782196 7 49 4.2937311535082685 7 50 1.4681215485920873 7 51 3.8541266006149293 7 52 3.1381775939980514 7 53 3.239479426881182 7 54 4.471092200517965 7 55 2.1434857994136536 7 56 2.0817732737627637 8 1 2.454490616931203 8 2 3.148401837839643 8 3 3.213390239559793 8 4 3.58547004632554 8 5 3.3253531529823874 8 6 5.57835861299565 8 7 3.3521604365871553 8 8 3.1618962985679318 8 9 2.6553516761059726 8 10 3.548261121458175 8 11 3.6319756487682255 8 12 5.77636380806041 8 13 6.109721263581875 8 14 6.827263482189352 8 15 6.156576172469855 8 16 5.998058338726736 8 17 4.517621696892718 8 18 5.892725862609424 8 19 4.769045184679264 8 20 4.825995898542196 8 21 4.4751559942016925 8 22 4.494259975309454 8 23 4.358311476715109 8 24 4.240465627724518 8 25 4.51611490049288 8 26 3.9449452154148332 8 27 4.036400597308543 8 28 3.6416152062756453 8 29 3.51376327907613 8 30 4.169670734643709 8 31 3.7311809453928504 8 32 3.2413788040376446 8 33 3.693099564078821 8 34 4.239657511876537 8 35 4.241804902722151 8 36 4.253086150193134 8 37 4.856302068074349 8 38 4.376709938961106 8 39 4.973617068530174 8 40 7.434825919063378 8 41 -2.0917658193198747 8 42 6.917681127229319 8 43 7.389603319209012 8 44 7.663262495921913 8 45 5.981059751098716 8 46 7.12324099821096 8 47 7.749271243081349 8 48 7.680358049284589 8 49 10.373306750325241 8 50 7.86467327273002 8 51 8.35280034066798 8 52 8.217753190815024 8 53 8.756720806191527 8 54 8.5307738981307 8 55 7.292026913472007 8 56 4.142781231322445 9 1 1.8391060015465897 9 2 2.263786453224263 9 3 2.328774854944413 9 4 2.9316238924793865 9 5 2.9407377683670077 9 6 5.424512459149497 9 7 2.5444681288948487 9 8 2.738819375491005 9 9 2.5784285991828995 9 10 3.048261121458175 9 11 3.362744879537459 9 12 5.545594577291176 9 13 6.109721263581875 9 14 6.481109636035505 9 15 5.887345403239088 9 16 4.113442954111349 9 17 5.902237081508105 9 18 5.623495093378651 9 19 4.615199030833111 9 20 4.749072821619116 9 21 3.9751559942016925 9 22 4.494259975309454 9 23 4.050619169022802 9 24 4.317388704647598 9 25 2.2853456697236467 9 26 1.4834067538763733 9 27 1.0364005973085426 9 28 1.526230590891032 9 29 1.8214555867684368 9 30 1.5542861192590962 9 31 1.461950176162084 9 32 1.3567634194222578 9 33 1.4238687948480546 9 34 1.8550421272611501 9 35 1.587958748875998 9 36 1.791547688654667 9 37 2.1639943757666558 9 38 1.761325323576493 9 39 2.5890016839147947 9 40 5.550210534447999 9 41 1.6390034114493552 9 42 3.8407580503062455 9 43 5.581911011516706 9 44 4.278647111306533 9 45 3.2502905203294823 9 46 4.08477945974942 9 47 3.018502012312119 9 48 4.026511895438436 9 49 6.950229827248322 9 50 4.710827118883866 9 51 3.737415725283366 9 52 4.871599344661178 9 53 2.9105669600376807 9 54 4.18462005197685 9 55 5.330488451933547 9 56 5.835088923630138 10 1 1.6714136938542836 10 2 2.725324914762723 10 3 2.9503133164828697 10 4 2.7839315847870765 10 5 3.169968537597775 10 6 4.978358612995649 10 7 2.3690835135102333 10 8 2.2557424524140828 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35 1.1795338404510858 115 36 2.950888347995331 115 37 3.05593576770805 115 38 2.882204444455617 115 39 3.7886353835484954 115 40 6.509917494154955 115 41 7.302007074453023 115 42 2.9909412004893916 115 43 -6.130543200937506 115 44 -9.248825416165996 115 45 2.7979095679485333 115 46 -1.464671089701131 115 47 -0.2598862660761583 115 48 -0.45334158441504613 115 49 -12.30068592366743 115 50 -0.8734219653652175 115 51 2.1513351392027786 115 52 1.7342367072985425 115 53 1.137673187143907 115 54 -7.093768226411427 115 55 0.5704151918602882 115 56 1.933990022531237 116 1 1.406413693854283 116 2 1.725324914762723 116 3 1.5819799831495374 116 4 1.3739315847870799 116 5 1.0849685375977742 116 6 4.870025279662315 116 7 1.4707501801768998 116 8 1.0240757857474208 116 9 1.472659368413666 116 10 1.6380047112017593 116 11 -0.31674229994972336 116 12 -11.111456704760105 116 13 -5.630663351802738 116 14 -1.9611980562721882 116 15 -7.56778280188912 116 16 -3.6077108920424976 116 17 -1.7067372774662566 116 18 -7.963043368159809 116 19 -2.3591599435258637 116 20 -3.049004101457804 116 21 1.580925224970926 116 22 2.2634907445402206 116 23 1.9127986562022912 116 24 2.150722037980934 116 25 1.7821405415185225 116 26 1.6372529077225266 116 27 1.177426238334185 116 28 0.5743075139679519 116 29 1.010558150871006 116 30 0.9356963756693517 116 31 1.5100270992390037 116 32 1.9689429066017468 116 33 1.6097662307454854 116 34 2.1435036657226902 116 35 2.3700100309272756 116 36 3.688983586090565 116 37 2.2345071962794734 116 38 2.8702996825508507 116 39 4.175540145453255 116 40 6.736107970345429 116 41 4.92105469350064 116 42 -2.181677847129656 116 43 -7.809114629508933 116 44 -3.1893016066421858 116 45 0.2502905203294823 116 46 -2.3575282325582734 116 47 -1.295600551790443 116 48 -3.8164368225102834 116 49 -6.854257352238857 116 50 -2.5103267272699803 116 51 -4.759379146511506 116 52 -4.801477578415742 116 53 -5.243279193808473 116 54 -9.962815845459048 116 55 -5.852203855758759 116 56 -3.8636290250878105 117 1 1.031413693854283 117 2 1.8364360258738373 117 3 1.790313316482873 117 4 2.235042695898194 117 5 2.2655240931533314 117 6 4.689469724106765 117 7 1.7068612912880141 117 8 1.6490757857474208 117 9 1.903214923969223 117 10 1.8046713778684307 117 11 -0.5389645221719448 117 12 -9.90312337142677 117 13 -9.616774462913849 117 14 -9.100086945161078 117 15 -5.706671690778009 117 16 -13.843822003153608 117 17 -8.956737277466257 117 18 -15.115821145937588 117 19 -5.748048832414753 117 20 -6.118448545902247 117 21 0.8170363360820403 117 22 1.0412685223179992 117 23 -0.12886801046438023 117 24 0.9840553713142626 117 25 1.1015849859629654 117 26 2.5816973521669695 117 27 2.9552040161119635 117 28 2.615974180634616 117 29 2.1633359286487845 117 30 3.3662519312249017 117 31 1.7739159881278894 117 32 3.3439429066017468 117 33 3.026432897412157 117 34 1.1157258879449117 117 35 2.147787808705054 117 36 2.4667613638683434 117 37 3.5400627518350305 117 38 2.5091885714397435 117 39 2.661651256564369 117 40 7.597219081456544 117 41 2.6432769157228613 117 42 -3.1677889582407666 117 43 -7.225781296175601 117 44 -9.772634939975521 117 45 -2.305265035226075 117 46 -3.329750454780495 117 47 -3.4344894406793323 117 48 -5.13588126695473 117 49 -7.451479574461082 117 50 -4.079771171714423 117 51 -6.912156924289285 117 52 -6.71814424508241 117 53 -6.076612527141805 117 54 -12.212815845459046 117 55 -4.58831496686987 117 56 -3.3358512473100355 118 1 0.737296046795457 118 2 1.431207267703897 118 3 1.378548610600518 118 4 1.918049231845906 118 5 1.9158508905389482 118 6 4.166593907113295 118 7 1.2232011605690616 118 8 0.9922130406493821 118 9 1.494718191943079 118 10 1.647808632770392 118 11 -0.8657619077928587 118 12 -6.569790038093437 118 13 -6.682133940038032 118 14 -10.50531570333101 118 15 -6.445233782281274 118 16 -17.37976971557191 118 17 -13.790070610799589 118 18 -14.33804336815981 118 19 -6.486610923918018 118 20 -5.174004101457804 118 21 1.000042872029752 118 22 1.4399613327755176 118 23 0.9397594405160135 118 24 -0.1531995306465177 118 25 0.35321897289107085 118 26 1.3725470253695846 118 27 1.2264458461773202 118 28 2.331660455144423 118 29 2.0816365822435543 118 30 3.212657159983074 118 31 1.9144388639448877 118 32 -3.0482139561433534 118 33 2.575452505255292 118 34 3.1287977833697482 118 35 2.4680492466135533 118 36 1.2870228017768426 118 37 0.05313464725986705 118 38 -0.31107286646875565 118 39 0.37406955721795754 118 40 4.437088362502294 118 41 2.3132115562457365 118 42 -3.9782464745806365 118 43 -3.9316636491167785 118 44 -5.988321214485325 118 45 -4.54382712672934 118 46 -8.548704703146509 118 47 -9.741678983162995 118 48 -11.887515253882835 118 49 -5.807688724787877 118 50 -7.870620844917038 118 51 -4.859869342589938 118 52 -5.139712872533391 118 53 -5.007985076161415 118 54 -10.92850211996885 118 55 -4.241909738111701 118 56 -2.3097074564603624 119 1 1.0869692494098402 119 2 1.7808804703182801 119 3 1.8458688720384302 119 4 2.679487140342637 119 5 2.2655240931533314 119 6 4.6339141685512075 119 7 1.9846390690657927 119 8 1.8712980079696422 119 9 1.903214923969223 119 10 2.415782488979538 119 11 -2.4834089666163877 119 12 -8.569790038093437 119 13 -8.172330018469406 119 14 -12.93342027849441 119 15 -7.706671690778009 119 16 -15.17715533648694 119 17 -15.456737277466257 119 18 -17.06026559038203 119 19 -6.192493276859196 119 20 -7.007337434791136 119 21 0.705925224970926 119 22 2.7634907445402206 119 23 1.982243100646734 119 24 2.206277593536484 119 25 1.2682516526296297 119 26 2.6372529077225266 119 27 3.121870682778628 119 28 2.1715297361901733 119 29 2.941113706426556 119 30 3.1440297090026803 119 31 1.6072493214612251 119 32 -3.933834871176032 119 33 1.7486551196343783 119 34 0.8935036657226902 119 35 1.536676697593947 119 36 3.244539141646122 119 37 4.151173862946145 119 38 3.8980774603286292 119 39 4.272762367675476 119 40 8.319441303678765 119 41 6.309943582389529 119 42 1.9433221528703442 119 43 -6.392447962842269 119 44 -6.161523828864411 119 45 -0.027487257448296276 119 46 -15.551972677002718 119 47 -15.434489440679332 119 48 -11.358103489176951 119 49 -14.118146241127747 119 50 -11.413104505047759 119 51 -8.745490257622617 119 52 -7.495922022860185 119 53 -6.6877236382529155 119 54 -14.65726028990349 119 55 -8.643870522425427 119 56 -5.058073469532257 120 1 -0.23525297281238267 120 2 -0.008008418570611298 120 3 -0.3430200168504598 120 4 0.8905982514537456 120 5 -0.12336479573556147 120 6 1.5116919463289804 120 7 -0.2042498198230973 120 8 0.015742452414087893 120 9 0.4476593684136674 120 10 -0.19532862213156932 120 11 -4.583408966616389 120 12 -10.70312337142677 120 13 -14.372330018469405 120 14 -15.077864722938855 120 15 -13.084449468555786 120 16 -16.932710892042497 120 17 -11.790070610799589 120 18 -20.671376701493145 120 19 -10.525826610192528 120 20 -12.374004101457803 120 21 -5.960741441695742 120 22 -4.53650925545978 120 23 -4.428868010464377 120 24 -8.615944628685735 120 25 -2.709526125148148 120 26 0.9372529077225238 120 27 -0.055907094999149365 120 28 0.0493075139679533 120 29 1.6188914842043403 120 30 0.6440297090026803 120 31 -1.6149729007609963 120 32 -14.189390426731588 120 33 -1.9069004359211803 120 34 -3.173163000943976 120 35 -1.8966566357393901 120 36 -2.544349747242766 120 37 -1.4488261370538567 120 38 -3.5463669841158136 120 39 -3.5827931878800783 120 40 0.5194413036787644 120 41 -2.745611973166028 120 42 -7.390011180462988 120 43 -13.8257812961756 120 44 -12.505968273308854 120 45 -11.183042813003851 120 46 -19.407528232558274 120 47 -14.078933885123778 120 48 -15.224770155843618 120 49 -19.262590685572192 120 50 -14.168660060603314 120 51 -14.134379146511508 120 52 -13.618144245082409 120 53 -12.309945860475139 120 54 -18.312815845459046 120 55 -14.677203855758759 120 56 -12.980295691754478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 180.0 5 9.0 6 18.0 7 23.0 8 29.0 9 22.0 10 17.0 11 25.0 12 20.0 13 38.0 14 50.0 15 47.0 16 68.0 17 79.0 18 124.0 19 146.0 20 171.0 21 216.0 22 285.0 23 327.0 24 379.0 25 440.0 26 504.0 27 528.0 28 638.0 29 688.0 30 813.0 31 957.0 32 1117.0 33 1262.0 34 1523.0 35 1792.0 36 2330.0 37 2814.0 38 2622.0 39 1568.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.47416552354824 0.3429355281207133 0.04572473708276177 0.1371742112482853 8 99.66163694558756 0.15546410608139005 0.05029721079103795 0.13260173754000915 9 99.04892546867855 0.5761316872427984 0.17832647462277093 0.19661636945587563 10 49.794238683127574 37.846364883401925 6.378600823045268 5.98079561042524 11 32.69318701417467 26.28257887517147 26.428898033836308 14.595336076817558 12 43.85916780978509 11.86556927297668 20.45267489711934 23.822588020118886 13 21.069239915851092 24.197384066587396 23.74919967072167 30.984176346839842 14 31.867026384379717 16.713155608395446 26.837075312085602 24.58274269513924 15 24.197689345314505 25.802310654685495 24.49110581331377 25.508894186686227 16 26.4063715841992 16.826108847896737 32.94731542088159 23.820204147022476 17 36.01737540009145 18.390489254686784 22.565157750342934 23.02697759487883 18 35.88020118884317 19.346136259716506 22.066758116140832 22.706904435299496 19 27.196595277320153 31.777411678564892 19.12868387332967 21.897309170785284 20 25.696764450139582 22.319344652418653 19.843485424008055 32.14040547343371 21 24.288980338363054 19.82167352537723 33.726566072245085 22.16278006401463 22 23.662551440329217 20.868770004572472 29.716506630086876 25.75217192501143 23 24.741655235482398 30.576131687242796 22.359396433470508 22.3228166438043 24 23.284100781928757 31.080524943984635 22.55704421784261 23.078330056244 25 24.788513420824 31.318304449220356 22.241529105125977 21.651653024829667 26 27.054465633145835 20.86705995335437 28.842548131888236 23.23592628161156 27 31.256005490734385 18.531228551818806 25.362617250057195 24.850148707389614 28 20.98494210261339 29.534532472882052 27.534440935511924 21.946084488992632 29 24.195023783388216 27.762532016099524 27.36919136480059 20.673252835711672 30 21.50827769139303 18.718558492636973 40.771060093295524 19.00210372267447 31 20.91853071680161 20.067700471158684 40.26348291477974 18.75028589725996 32 33.203661327231124 18.18306636155606 31.1350114416476 17.47826086956522 33 23.64660621049739 25.199230557845564 32.77915178162499 18.375011450032062 34 19.71811650574292 19.969798197043882 35.38644579691576 24.925639500297443 35 27.51475229861397 19.367823978774986 37.610356342344815 15.507067380266227 36 28.909930927221993 20.721833401948675 34.80170166049128 15.566534010338046 37 28.1586298484224 19.315840087924165 34.5835050602189 17.94202500343454 38 22.274685981479784 21.041532960484094 40.68488126890987 15.99889978912625 39 21.42005867253392 26.897689768976896 33.22790612394573 18.454345434543455 40 16.365059630704057 14.583045540360088 31.311875489248052 37.740019339687805 41 19.58187188480709 14.371423297027874 29.213586856193462 36.833117961971574 42 19.48896419085997 19.470647495191866 34.1881124645114 26.85227584943676 43 16.60323719565317 21.44068962354991 36.19148058141135 25.764592599385576 44 16.22528735632184 17.020689655172415 33.742528735632185 33.01149425287356 45 19.139479472140764 25.76521260997067 36.29490469208211 18.800403225806452 46 26.041714416685767 20.229200091680035 34.77882191152876 18.95026358010543 47 16.717742304349812 20.152769519279147 43.12308466358688 20.006403512784157 48 18.884002199010443 19.901044529961517 43.618288436870074 17.596664834157963 49 16.52097902097902 16.60379094589621 31.67556128082444 35.19966875230033 50 19.719793049768782 18.978068769744976 42.3332265006181 18.96891167986814 51 25.760007336420742 21.628685405107984 35.934705855380805 16.67660140309047 52 18.928571428571427 27.58699633699634 35.077838827838825 18.40659340659341 53 26.481905634448005 19.29454878607421 36.36738433348603 17.856161245991757 54 17.27664648688314 20.34489084571638 40.414602825169695 21.963859842230786 55 18.474024485304234 20.50071071575955 35.26525746251548 25.760007336420742 56 27.07731793391081 20.899216279389524 33.22792061964343 18.795545167056236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.5 11 1.0 12 2.0 13 10.0 14 17.0 15 33.0 16 49.0 17 56.0 18 63.0 19 100.5 20 138.0 21 175.0 22 226.5 23 241.0 24 287.0 25 333.0 26 428.0 27 523.0 28 579.0 29 656.0 30 677.0 31 684.5 32 692.0 33 693.5 34 695.0 35 645.0 36 595.0 37 501.5 38 384.5 39 361.0 40 319.0 41 277.0 42 270.0 43 263.0 44 285.0 45 307.0 46 363.5 47 552.5 48 685.0 49 882.0 50 1079.0 51 1142.0 52 1205.0 53 1091.5 54 944.0 55 910.0 56 933.0 57 956.0 58 1011.5 59 1067.0 60 1135.5 61 1204.0 62 1240.5 63 1307.0 64 1337.0 65 1237.0 66 1137.0 67 1031.0 68 925.0 69 809.0 70 693.0 71 588.0 72 420.0 73 357.0 74 317.0 75 277.0 76 230.0 77 183.0 78 144.0 79 77.0 80 49.0 81 43.0 82 37.0 83 23.5 84 10.0 85 6.5 86 3.0 87 1.5 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01828989483310471 14 0.004572473708276178 15 0.2652034750800183 16 0.10516689529035209 17 0.0 18 0.0 19 0.0823045267489712 20 0.08687700045724737 21 0.0 22 0.0 23 0.0 24 0.004572473708276178 25 0.004572473708276178 26 0.013717421124828533 27 0.06858710562414265 28 0.09602194787379972 29 0.027434842249657067 30 0.01828989483310471 31 0.0411522633744856 32 0.09144947416552354 33 0.1646090534979424 34 0.07773205304069503 35 0.0411522633744856 36 0.0411522633744856 37 0.15089163237311387 38 0.25605852766346593 39 0.24691358024691357 40 0.6995884773662552 41 0.9236396890717878 42 0.1463191586648377 43 0.27892089620484684 44 0.5486968449931412 45 0.21033379058070417 46 0.2514860539551898 47 0.03200731595793324 48 0.19204389574759945 49 0.6127114769090077 50 0.13260173754000915 51 0.27892089620484684 52 0.1371742112482853 53 0.18289894833104708 54 0.30178326474622774 55 0.27892089620484684 56 0.23319615912208505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 21870.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.09693644261546 #Duplication Level Percentage of deduplicated Percentage of total 1 86.62758407898797 64.18838591678097 2 7.824745448935514 11.595793324188385 3 2.462203023758099 5.473251028806585 4 1.0182042579450787 3.017832647462277 5 0.660290033940142 2.446273433927755 6 0.3949398333847578 1.7558299039780523 7 0.271521135452021 1.4083219021490627 8 0.1419315026226473 0.8413351623228167 9 0.12958963282937366 0.8641975308641975 >10 0.425794507867942 5.052583447645176 >50 0.030854674483184203 1.7375400091449476 >100 0.012341869793273681 1.6186556927297666 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 183 0.8367626886145405 No Hit CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 171 0.7818930041152263 No Hit CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 90 0.411522633744856 No Hit CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 86 0.39323273891175126 No Hit CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 77 0.3520804755372657 No Hit CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 64 0.2926383173296754 No Hit CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 63 0.2880658436213992 No Hit CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 50 0.2286236854138089 No Hit CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 43 0.19661636945587563 No Hit CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 40 0.18289894833104708 No Hit CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 39 0.17832647462277093 No Hit CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 36 0.1646090534979424 No Hit CTCGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33 0.15089163237311387 No Hit CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 33 0.15089163237311387 No Hit CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 23 0.10516689529035209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.004572473708276178 0.0 0.0 0.0 0.0 7 0.004572473708276178 0.0 0.0 0.0 0.0 8 0.004572473708276178 0.0 0.0 0.0 0.0 9 0.004572473708276178 0.0 0.0 0.0 0.0 10 0.009144947416552356 0.0 0.0 0.0 0.0 11 0.01828989483310471 0.0 0.0 0.0 0.0 12 0.01828989483310471 0.0 0.0 0.0 0.0 13 0.05029721079103795 0.0 0.0 0.0 0.0 14 0.05944215820759031 0.0 0.0 0.0 0.0 15 0.07315957933241884 0.0 0.0 0.0 0.0 16 0.07315957933241884 0.0 0.0 0.0 0.0 17 0.07773205304069501 0.0 0.0 0.0 0.0 18 0.0823045267489712 0.0 0.0 0.0 0.0 19 0.08687700045724737 0.0 0.0 0.0 0.0 20 0.09144947416552354 0.0 0.0 0.0 0.0 21 0.1005944215820759 0.0 0.0 0.0 0.0 22 0.1005944215820759 0.0 0.0 0.0 0.0 23 0.10516689529035209 0.0 0.0 0.0 0.0 24 0.10973936899862825 0.0 0.0 0.0 0.0 25 0.10973936899862825 0.0 0.0 0.0 0.0 26 0.11431184270690443 0.0 0.0 0.0 0.0 27 0.12345679012345678 0.0 0.0 0.0 0.0 28 0.12802926383173296 0.0 0.0 0.0 0.0 29 0.13717421124828533 0.0 0.0 0.0 0.0 30 0.13717421124828533 0.0 0.0 0.0 0.0 31 0.1463191586648377 0.0 0.0 0.0 0.0 32 0.1463191586648377 0.0 0.0 0.0 0.0 33 0.1463191586648377 0.0 0.0 0.0 0.0 34 0.1463191586648377 0.0 0.0 0.0 0.0 35 0.15089163237311384 0.0 0.0 0.0 0.0 36 0.15546410608139002 0.0 0.0 0.0 0.0 37 0.15546410608139002 0.0 0.0 0.0 0.0 38 0.15546410608139002 0.0 0.0 0.0 0.0 39 0.1600365797896662 0.0 0.0 0.0 0.0 40 0.1600365797896662 0.0 0.0 0.0 0.0 41 0.1600365797896662 0.0 0.0 0.0 0.0 42 0.16918152720621857 0.0 0.0 0.0 0.0 43 0.17832647462277093 0.0 0.0 0.0 0.0 44 0.17832647462277093 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTTTT 25 1.8196035E-5 50.88498 39 CCCACAG 40 2.1191227E-9 50.88498 40 ACCCACA 40 2.1191227E-9 50.88498 39 TACCCAC 40 2.1191227E-9 50.88498 38 ACGCCCA 35 4.32683E-8 50.884975 39 TACGCCC 35 4.32683E-8 50.884975 38 TCCAAGT 15 0.007761206 50.884975 38 CCAAGTG 15 0.007761206 50.884975 39 CCGACGG 15 0.007761206 50.884975 39 CAAGTGG 15 0.007761206 50.884975 40 TGCCGAC 15 0.007833732 50.76581 37 GTACCCA 30 9.0039794E-7 50.76581 37 GACGGGC 15 0.007833732 50.76581 41 CTCCAAG 15 0.007833732 50.76581 37 GTACGCC 35 4.4083208E-8 50.76581 37 TTTTCCT 25 1.8972021E-5 50.529137 36 ACTCCAA 15 0.007980296 50.529137 36 GGTACCC 35 4.5749402E-8 50.529137 36 AAGAGCC 20 3.9299903E-4 50.411625 35 CGCTGAC 15 0.008128891 50.294662 47 >>END_MODULE