FastQCFastQC Report
Fri 17 Jun 2016
SRR1526271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53347
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.13309089545803887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGG150.008161372550.3456846
ATGTCAG150.008161372550.3456845
TTGTTTC150.00819209350.29791335
CCAGTAC204.0275764E-450.29791335
AGTACCA204.0275764E-450.29791337
TTGATAT150.00819209350.29791349
CCCAGTA204.046501E-450.25023734
ACATGTC150.00822289950.25023743
CCAAAAC309.939886E-750.25023743
CGACTCC204.046501E-450.25023744
CATGTCA150.00822289950.25023744
CAAAAAC150.00822289950.25023743
GCTCAAG150.00831583750.1077542
AACATGT150.00831583750.1077542
TAAAAAG150.0083469950.06043214
CTTAATC252.045688E-550.06043215
TCTTAAT252.045688E-550.06043214
TGATATG150.00837822850.01320650
CTCATGA150.00837822850.01320632
TCTCATG150.00837822850.01320631