##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53347
Sequences flagged as poor quality	0
Sequence length	56
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.452021669447205	39.0	38.0	39.0	34.0	40.0
2	36.71040545860124	39.0	38.0	40.0	31.0	40.0
3	36.4157684593323	39.0	38.0	39.0	30.0	40.0
4	36.00624215044895	39.0	35.0	39.0	29.0	40.0
5	36.60758805556076	39.0	38.0	40.0	30.0	40.0
6	36.207021950625155	39.0	36.0	39.0	30.0	40.0
7	36.98549121787542	39.0	38.0	40.0	33.0	40.0
8	36.85566198661593	39.0	38.0	40.0	32.0	40.0
9	36.83879130972688	39.0	38.0	40.0	32.0	40.0
10	36.413481545354	39.0	36.0	39.0	31.0	40.0
11	35.62910754119257	38.0	35.0	39.0	29.0	40.0
12	33.62983860385776	38.0	32.0	39.0	19.0	40.0
13	33.590792359457886	38.0	32.0	39.0	19.0	40.0
14	33.8211708249761	38.0	33.0	39.0	20.0	40.0
15	33.758843046469345	38.0	33.0	39.0	19.0	40.0
16	33.09520685324386	38.0	31.0	39.0	19.0	40.0
17	33.23982604457608	37.0	31.0	39.0	20.0	40.0
18	32.68303747164789	37.0	30.0	39.0	18.0	40.0
19	33.580782424503724	37.0	31.0	39.0	23.0	40.0
20	33.37094869439706	37.0	31.0	39.0	22.0	40.0
21	34.24066957842053	38.0	33.0	39.0	23.0	40.0
22	33.984591448441336	38.0	33.0	39.0	23.0	40.0
23	34.2220368530564	38.0	33.0	39.0	24.0	40.0
24	33.96134740472754	38.0	33.0	39.0	23.0	40.0
25	34.06493336082629	38.0	33.0	39.0	23.0	40.0
26	34.74626501958873	38.0	34.0	39.0	26.0	40.0
27	34.61113089770746	38.0	34.0	39.0	26.0	40.0
28	34.608450334601756	38.0	34.0	39.0	26.0	40.0
29	34.4106697658725	38.0	33.0	39.0	25.0	40.0
30	34.3551090033179	38.0	33.0	39.0	25.0	40.0
31	34.638705081822785	38.0	34.0	39.0	27.0	40.0
32	34.26880611843215	38.0	34.0	39.0	25.0	40.0
33	34.48169531557539	38.0	34.0	39.0	26.0	40.0
34	34.35975781206066	38.0	34.0	39.0	25.0	40.0
35	34.224286276641614	38.0	34.0	39.0	25.0	40.0
36	33.3198117982267	36.0	32.0	39.0	24.0	40.0
37	33.09541305040583	36.0	32.0	39.0	23.0	40.0
38	32.86900856655482	36.0	32.0	39.0	22.0	40.0
39	32.590173767971955	35.0	31.0	39.0	19.0	40.0
40	29.08879599602602	35.0	23.0	38.0	6.0	39.0
41	25.668678651095657	31.0	13.0	38.0	4.0	39.0
42	30.050012184377753	34.0	28.0	38.0	4.0	39.0
43	28.478152473428683	33.0	24.0	38.0	4.0	39.0
44	28.488631038296436	33.0	24.0	38.0	4.0	39.0
45	29.639098730950195	34.0	28.0	38.0	4.0	39.0
46	29.319249442330403	35.0	27.0	38.0	4.0	40.0
47	28.867827619172587	35.0	26.0	38.0	4.0	40.0
48	28.948713142257297	34.0	26.0	38.0	4.0	39.0
49	26.392130766491086	33.0	12.0	38.0	4.0	39.0
50	28.547453465049582	34.0	25.0	38.0	4.0	39.0
51	28.812229366224905	35.0	26.0	39.0	4.0	40.0
52	28.485631806849494	35.0	24.0	39.0	4.0	40.0
53	28.120344161808536	35.0	23.0	39.0	4.0	40.0
54	27.1373085646803	34.0	14.0	39.0	4.0	40.0
55	27.533582019607476	34.0	18.0	39.0	4.0	40.0
56	26.745402740547735	34.0	4.0	39.0	4.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.33032288727940085
1	2	1.5781612976608486
1	3	1.8260742747504537
1	4	2.065042788745025
1	5	1.797208074905221
1	6	1.720216837887648
1	7	1.2218179607090605
1	8	1.4772554184783715
1	9	1.40462331621233
1	10	1.2839649513781737
1	11	-4.00454797049429
1	12	-14.271245036475339
1	13	-9.513374422634946
1	14	-5.836726834497611
1	15	-5.691182381945406
1	16	-22.60634877854254
1	17	-5.298021335660376
1	18	-12.989521682326064
1	19	-5.495532905863836
1	20	-6.274276163500016
1	21	-5.348898661323574
1	22	-2.6978775717834083
1	23	-3.1271476046599602
1	24	-4.588689776969236
1	25	-3.307582498595057
1	26	2.522773606071702
1	27	1.8283740784676397
1	28	2.5328569490751107
1	29	1.9793008073135425
1	30	2.0309502751183075
1	31	1.4045576194596734
1	32	1.4609086428892724
1	33	1.1656419467253016
1	34	0.6415521675637166
1	35	1.270780131499869
1	36	1.7872592218277177
1	37	1.59802703692948
1	38	2.012865890163347
1	39	2.031179327142702
1	40	3.911756679562057
1	41	-9.532312805848328
1	42	-10.167611497072233
1	43	-15.460334512737358
1	44	-20.744131067821048
1	45	-10.376411903155436
1	46	-16.412735171222355
1	47	-21.916973374973576
1	48	-14.137967206805907
1	49	-19.34140641908603
1	50	-13.915871434092281
1	51	-10.676055705671974
1	52	-10.262781645753517
1	53	-10.2519202220758
1	54	-9.688101873980951
1	55	-9.584304733384617
1	56	-5.058543134354299
2	1	2.081231978188491
2	2	2.938161297660848
2	3	3.278801547477727
2	4	3.7529215766238124
2	5	3.2317535294506783
2	6	3.308701686372494
2	7	2.564848263739357
2	8	2.5754372366601928
2	9	2.624017255606276
2	10	2.7457831331963547
2	11	1.7093914234451049
2	12	-0.2476086728389788
2	13	-0.3667077559682781
2	14	3.9869095291387495
2	15	4.325181254418233
2	16	3.6736512214574617
2	17	4.478948361309321
2	18	-0.7713398641442453
2	19	2.7838610335301013
2	20	4.320875351651502
2	21	3.1565558841309738
2	22	2.9900012160953793
2	23	3.0540645165521596
2	24	2.118582950303491
2	25	3.233023562011006
2	26	2.923985727283828
2	27	3.1271619572555167
2	28	3.4728569490751156
2	29	2.742937170949908
2	30	3.3776169417849715
2	31	2.805769740671799
2	32	3.3681813701619987
2	33	2.8844298255131804
2	34	2.6973097433212914
2	35	3.73138619210593
2	36	3.4890774036459007
2	37	3.154390673293115
2	38	3.8649871022845588
2	39	4.3620884180517905
2	40	7.390544558349934
2	41	-6.0056461391816605
2	42	-5.914884224344959
2	43	-12.230031482434327
2	44	-13.54655531024529
2	45	-5.452775539519074
2	46	-9.109704868192054
2	47	-18.021215799216
2	48	-7.349482358321058
2	49	-12.386860964540578
2	50	-7.0716290098498575
2	51	-2.4566617662780352
2	52	-1.7900543730262441
2	53	-2.0991929493485273
2	54	-0.4808291467082242
2	55	-1.1800623091421905
2	56	2.1075174717063057
3	1	1.9853345422910564
3	2	3.0653407848403305
3	3	3.2270066756828584
3	4	3.7118959355981715
3	5	3.3543176320147836
3	6	3.2994709171417256
3	7	2.678694417585511
3	8	2.898001339224294
3	9	2.7927352043242237
3	10	2.5170651844784047
3	11	3.4022119362656156
3	12	5.874442609212309
3	13	5.012266603006083
3	14	4.732037734266953
3	15	5.240053049290026
3	16	5.675702503508738
3	17	5.21946118182214
3	18	5.931224238419858
3	19	4.546425136094207
3	20	5.022926633702781
3	21	4.696555884130973
3	22	4.346411472505636
3	23	4.340218362706004
3	24	4.023198334918874
3	25	4.714562023549469
3	26	3.6568062401043377
3	27	3.643059393152953
3	28	4.135421051639213
3	29	3.599347427360158
3	30	3.825822069990103
3	31	2.436026150928207
3	32	3.136899318879948
3	33	3.044429825513177
3	34	2.2429507689623165
3	35	2.5108733715931066
3	36	3.4690774036459047
3	37	4.144647083549529
3	38	4.045499922797376
3	39	4.263114059077431
3	40	7.508493276298655
3	41	8.711276937741417
3	42	3.8871670577063213
3	43	-1.263364815767659
3	44	1.9493421256521444
3	45	4.871839845096311
3	46	2.697987439500256
3	47	-2.2514722094724107
3	48	3.043850975012276
3	49	5.6644210867414735
3	50	2.585294067073221
3	51	2.865902336286066
3	52	3.2022533192814464
3	53	3.713114742959167
3	54	4.128914443035367
3	55	2.788655639575758
3	56	4.265466189655026
4	1	1.8656764226329372
4	2	2.369827964327513
4	3	3.2515793252555056
4	4	3.3251437988460353
4	5	2.9461979738951243
4	6	3.068701686372499
4	7	2.719292708183808
4	8	2.8509927922157416
4	9	2.844017255606275
4	10	2.3963386887519107
4	11	3.4032803123339903
4	12	5.17572466049436
4	13	4.917181132920611
4	14	5.151909529138749
4	15	4.361847921084895
4	16	4.963095665901903
4	17	5.371170583531544
4	18	5.384215691411306
4	19	5.138305477974548
4	20	4.7964309072070535
4	21	3.738222550797637
4	22	4.353890104984266
4	23	3.88295340544105
4	24	3.8746940614146013
4	25	3.878023562011002
4	26	2.005096838394934
4	27	2.457161957255515
4	28	2.880079171297332
4	29	3.120714948727681
4	30	2.6003947195627504
4	31	2.511880851782905
4	32	3.007625814606442
4	33	1.8777631588465127
4	34	0.8839764099879588
4	35	1.5674973032170385
4	36	3.1496329592014547
4	37	3.248279562182006
4	38	2.933320435617894
4	39	3.9415328624962385
4	40	6.5266556694610465
4	41	10.217687194151672
4	42	5.827337997877258
4	43	7.333857406454559
4	44	5.985666911976928
4	45	6.153891127147595
4	46	5.987517354030171
4	47	5.728228645228448
4	48	6.1827398639011655
4	49	8.627027924348308
4	50	6.925037656816812
4	51	5.791116011499742
4	52	5.607167849195978
4	53	5.840251495095917
4	54	5.705837519958443
4	55	4.673271024191141
4	56	4.964184138372975
5	1	1.747523058000695
5	2	2.596940640383849
5	3	2.8945761953650475
5	4	3.2731093700510385
5	5	2.770532872173682
5	6	3.115650043180011
5	7	2.0131111745374852
5	8	2.4691461568479838
5	9	2.250120541991251
5	10	2.395360597985089
5	11	3.2626308600648244
5	12	5.1416871018089125
5	13	4.92324529567491
5	14	4.923623144162228
5	15	4.614195338925271
5	16	5.750458733194549
5	17	5.3613896758633075
5	18	5.340397225057629
5	19	4.427429108647473
5	20	4.921626525360423
5	21	3.468856353614541
5	22	3.6792030940296527
5	23	3.827397849885493
5	24	3.7982073634490305
5	25	3.2835399939358894
5	26	2.451497464373027
5	27	3.0052840229832185
5	28	1.8028100007183028
5	29	3.141059236677606
5	30	2.6271944065737074
5	31	2.0797603510004308
5	32	2.645927849035239
5	33	2.039734989832425
5	34	1.691488147077159
5	35	1.7773955817772915
5	36	3.2977159012984814
5	37	3.1686629737626006
5	38	2.915127947354982
5	39	2.962464004906252
5	40	7.5980562954391395
5	41	9.024025222320688
5	42	4.515913897720765
5	43	5.809405137283985
5	44	6.008749854073955
5	45	3.9261915966311633
5	46	3.5710463055168695
5	47	3.8371879566525458
5	48	3.7785927590498325
5	49	7.387786922783366
5	50	4.060014182638405
5	51	2.2633382337219636
5	52	3.2540770823728167
5	53	2.105314092904994
5	54	2.4335370504748752
5	55	0.4620034185573374
5	56	2.561601978748559
6	1	2.0688510258075397
6	2	3.1733993928989435
6	3	3.484515833192013
6	4	3.9053025290047643
6	5	3.4041344818316333
6	6	3.1782254958963065
6	7	2.6724673113584103
6	8	2.7478181890411406
6	9	2.9106839222729377
6	10	3.0514974189106425
6	11	4.132248566302245
6	12	5.920962755732454
6	13	6.043768434507911
6	14	5.774528576757795
6	15	5.259466968703947
6	16	5.9888893166955555
6	17	5.322757885118847
6	18	5.809612516808137
6	19	4.710527700196771
6	20	5.093256304032451
6	21	3.791793979369068
6	22	4.985239311333473
6	23	4.541683564171208
6	24	4.848106759827303
6	25	4.7577854667729085
6	26	3.453509536807637
6	27	3.4690667191602813
6	28	3.294761710979877
6	29	3.6619847899975255
6	30	4.026188370356401
6	31	2.8352935501956082
6	32	3.6738956558762865
6	33	3.2920488731322237
6	34	3.2363573623689064
6	35	2.850433811153543
6	36	4.23574407031257
6	37	4.701057339959782
6	38	4.167844245141701
6	39	5.298278894242266
6	40	8.571496939302317
6	41	4.6676871941516715
6	42	6.867020537559803
6	43	7.86996851756567
6	44	7.746778023088041
6	45	6.949129222385686
6	46	7.256961798474617
6	47	7.749260391260194
6	48	6.948612879774178
6	49	10.17028189260228
6	50	6.041704323483479
6	51	5.620481090864818
6	52	5.733755150783278
6	53	6.363664193508615
6	54	7.782027996148916
6	55	5.958985309905426
6	56	7.5046603288491625
7	1	2.0010441847612626
7	2	2.695532189679625
7	3	2.641055068604487
7	4	2.7801516235721664
7	5	2.7846173792159377
7	6	2.3691711699405715
7	7	2.4919844139741016
7	8	2.6522447483972797
7	9	2.1796980067799794
7	10	2.5643746824921294
7	11	3.7696731135859523
7	12	5.578306820118769
7	13	5.599301633703085
7	14	5.8109470878241964
7	15	5.684617874136542
7	16	5.722289719110037
7	17	5.783924887130915
7	18	6.157298633508333
7	19	5.441513615689729
7	20	5.654020891557607
7	21	4.187166212769469
7	22	4.045400277128245
7	23	3.8978203850967574
7	24	4.854545391618046
7	25	4.283539993935889
7	26	3.0571312671899307
7	27	3.6390868398846266
7	28	3.5352043669154867
7	29	3.2396507859733816
7	30	3.866631026292012
7	31	3.4459575340990227
7	32	3.505082778612703
7	33	3.448185694057777
7	34	3.6210656118658875
7	35	3.8759871310730603
7	36	4.635744070312569
7	37	5.027817903340065
7	38	4.182733581157798
7	39	4.7652809063147
7	40	8.048760520791252
7	41	2.066278743447448
7	42	6.36098432025598
7	43	7.809405137283985
7	44	7.543961121679594
7	45	5.6022479346593315
7	46	6.627384333685878
7	47	6.9639485200328295
7	48	6.088451913979409
7	49	8.796237627008718
7	50	6.891000098131364
7	51	6.235169219637459
7	52	6.310415110541829
7	53	5.908130994313442
7	54	6.10959338850304
7	55	5.884538629824942
7	56	5.660193528044335
8	1	1.9936957463044322
8	2	2.8680163701246144
8	3	2.9756131416806255
8	4	3.352921576623814
8	5	2.983637587421697
8	6	2.604933570430468
8	7	2.5683265246089277
8	8	2.4838430337616373
8	9	2.5759013135772904
8	10	2.3933193650804085
8	11	3.5777972205465574
8	12	5.9547101677407355
8	13	5.1677849976549055
8	14	5.6066196740662875
8	15	5.821992848621129
8	16	5.683506293921226
8	17	5.790252709135409
8	18	5.828660135855756
8	19	5.045310308892418
8	20	5.197397090781934
8	21	4.573367478333871
8	22	4.256667882762045
8	23	3.508557270175345
8	24	4.913945269144072
8	25	4.579110518532744
8	26	3.8758697852548423
8	27	3.714408334067109
8	28	3.887929412843228
8	29	3.893661808631066
8	30	3.552689405553089
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115	33	1.6130572764935707
115	34	1.4133881746938357
115	35	1.7039352117137696
115	36	2.2043715212929627
115	37	1.7638024379989972
115	38	2.6563596513041645
115	39	4.0044413592282595
115	40	7.045446519134249
115	41	5.752000919641869
115	42	1.8011942070275886
115	43	-7.194737364787269
115	44	-8.45714354553941
115	45	0.3597734800887693
115	46	-5.5214695740744055
115	47	-5.694156975686589
115	48	-6.240462750477921
115	49	-13.033919788069989
115	50	-4.932805480438091
115	51	-4.226857844709407
115	52	-3.9116230004772277
115	53	-3.9399772630740166
115	54	-7.960829146708225
115	55	-4.8169250542402295
115	56	-1.8250315479015349
116	1	1.369319409882479
116	2	1.8841722266225958
116	3	2.631588432723632
116	4	2.937621030175727
116	5	2.270879212510785
116	6	2.4566798284489977
116	7	1.8581815970726936
116	8	2.076858001687512
116	9	2.027077364895888
116	10	2.2198814938520925
116	11	-2.020444642128666
116	12	-13.063346377757007
116	13	-8.283866225913634
116	14	-3.6069702522820144
116	15	-8.276130220991607
116	16	-3.546239488925057
116	17	-2.606516119564997
116	18	-8.652214181084137
116	19	-3.3894722998032307
116	20	-4.092457981681832
116	21	2.2074848458796055
116	22	2.8304383745653254
116	23	2.729037194147786
116	24	2.3872168300849097
116	25	2.906029026491879
116	26	1.7532753447701666
116	27	2.278200208621641
116	28	1.5137312660150073
116	29	1.9549590288734038
116	30	2.2222071057193986
116	31	0.8153872270105964
116	32	2.3603125177029796
116	33	2.0171074211415956
116	34	1.796544715998884
116	35	1.9598014926523746
116	36	3.4718096440830593
116	37	3.34368029077946
116	38	3.110233003923902
116	39	3.6357496202375827
116	40	7.375244011901852
116	41	4.377523259725443
116	42	-1.0615508910116276
116	43	-10.134949515221212
116	44	-8.702402304780811
116	45	-1.4499340094644282
116	46	-5.393857873656533
116	47	-3.548866072440042
116	48	-4.171996019523245
116	49	-8.780303587491396
116	50	-4.18288584045095
116	51	-3.0973175039829535
116	52	-2.208633607998923
116	53	-3.5365699985288543
116	54	-8.436238982773798
116	55	-3.3759093036777124
116	56	-3.567236626654349
117	1	1.0500198569763697
117	2	1.9493734188729661
117	3	2.23516518384136
117	4	2.6104973341995716
117	5	2.1684201961173457
117	6	2.076277443948257
117	7	1.4718179607090605
117	8	1.6211948124177624
117	9	1.692502104091119
117	10	1.9430558604690802
117	11	-2.0424267583730753
117	12	-8.316699581929885
117	13	-8.308828968089493
117	14	-12.457938955709732
117	15	-9.236636927399953
117	16	-14.553318475512238
117	17	-12.093475881114923
117	18	-12.072855015659396
117	19	-5.366745027075957
117	20	-5.433367072590922
117	21	1.5223134598885437
117	22	2.2112133373074983
117	23	1.5546705771582197
117	24	1.8734314351519785
117	25	1.9272659862534312
117	26	3.3333796666777644
117	27	3.8359498360433975
117	28	3.5480084642266263
117	29	3.494452322465058
117	30	3.7885260326940653
117	31	2.9651636800657357
117	32	2.3472722792529055
117	33	2.6732177043010594
117	34	2.7097339857455296
117	35	2.869264979984713
117	36	4.113016797585296
117	37	4.446511885414331
117	38	4.4295325568300115
117	39	4.091785387748757
117	40	8.61630213410751
117	41	4.399505375969856
117	42	-0.9479145273752643
117	43	-8.88457693697978
117	44	-9.494131067821048
117	45	0.7978305210869863
117	46	-2.420310928798113
117	47	-2.9624279204281194
117	48	-2.827361146199845
117	49	-8.705042782722394
117	50	-2.7492047674256135
117	51	-1.9184799480962198
117	52	-1.3233877063595791
117	53	-3.5928293129848896
117	54	-8.279010964890041
117	55	-3.031274430354312
117	56	-1.8161188919300564
118	1	0.6101208670773772
118	2	1.48927240877196
118	3	1.8876904363666185
118	4	2.1084771321793667
118	5	1.7295313072284557
118	6	2.2242572419280506
118	7	1.3470704859615807
118	8	1.4732150144379688
118	9	1.4106839222729377
118	10	1.5768942443074678
118	11	-2.2994974654437854
118	12	-9.340942006172309
118	13	-10.24670775596828
118	14	-9.528646026416805
118	15	-7.088152078915105
118	16	-13.450793222986988
118	17	-11.178829416468457
118	18	-12.371339864144247
118	19	-6.345027855358786
118	20	-5.603569092792945
118	21	0.6187781063531901
118	22	1.2122234383176007
118	23	0.9162867387743816
118	24	1.1496940614145998
118	25	0.7085791175665577
118	26	2.7773190606171596
118	27	2.5627175128110764
118	28	2.5439680601862236
118	29	2.542937170949905
118	30	2.8309502751183047
118	31	0.14799196289401806
118	32	-5.156263074282446
118	33	0.8222076032909555
118	34	1.5061986322101788
118	35	1.3758306365503685
118	36	1.7690774036459018
118	37	2.1010573399597874
118	38	2.358320435617891
118	39	2.47764397360735
118	40	6.346100113905489
118	41	1.4565760830405594
118	42	-5.08377311323385
118	43	-10.030031482434328
118	44	-8.230999754689737
118	45	-6.212775539519075
118	46	-12.243038201525387
118	47	-12.163438021438221
118	48	-13.856149024987724
118	49	-11.031305408985022
118	50	-9.969406787627634
118	51	-8.758883988500259
118	52	-8.990054373026247
118	53	-8.979192949348526
118	54	-12.671940257819335
118	55	-8.637840086919969
118	56	-5.6747047505159145
119	1	0.8259938829503923
119	2	1.8162565357560823
119	3	2.1702301189062965
119	4	2.672649467780275
119	5	2.1531140736683696
119	6	2.34761325099835
119	7	1.4908346582971816
119	8	1.4927161482248152
119	9	1.5208880039055899
119	10	1.4249668066657435
119	11	-5.814690209207956
119	12	-13.22393520345122
119	13	-11.839905034879845
119	14	-11.966287749772814
119	15	-12.28951262313279
119	16	-14.44988619350853
119	17	-14.83234415569748
119	18	-16.54548952400819
119	19	-7.634370258986905
119	20	-8.582253900049178
119	21	-0.19800193899828145
119	22	0.7464638011293943
119	23	0.9090305029467203
119	24	0.5297394128885244
119	25	0.35166301779331377
119	26	1.9228972919096776
119	27	1.9690667191602813
119	28	1.1825168130206904
119	29	2.676270504283238
119	30	2.411902656070687
119	31	0.8046813052976489
119	32	-4.393451282899225
119	33	0.9900080568056993
119	34	1.0404389950219723
119	35	0.6034950356433413
119	36	1.7173767233737962
119	37	2.5745267277148898
119	38	2.286211592080477
119	39	2.700319710568799
119	40	5.457211225016604
119	41	0.2942178063965706
119	42	-4.122775380807546
119	43	-11.899419237536367
119	44	-8.02056891568747
119	45	-5.94474832863472
119	46	-16.54507901785192
119	47	-15.109923282209197
119	48	-13.367713650838065
119	49	-13.774616066581395
119	50	-11.14809159488387
119	51	-10.859110745869874
119	52	-10.26216321656366
119	53	-10.571029684042404
119	54	-13.124094452830674
119	55	-9.408361628870082
119	56	-8.085135589518181
120	1	0.5518626088191212
120	2	1.3259090454085936
120	3	1.7609637096398885
120	4	1.8303990541012922
120	5	1.4454472231443702
120	6	1.9245575422283494
120	7	0.9392626781537743
120	8	1.1512930925160418
120	9	1.0106839222729391
120	10	1.06037772779095
120	11	-4.0160139819603025
120	12	-14.002203267433568
120	13	-14.080941990202515
120	14	-14.126243624014403
120	15	-10.608872799635822
120	16	-15.507249679443444
120	17	-13.583033620672662
120	18	-16.39656508936947
120	19	-6.930012840343771
120	20	-13.38975527897913
120	21	-5.298939611364524
120	22	-3.094683468589306
120	23	-2.6861156636280192
120	24	-10.559615247894705
120	25	-2.1839133749259325
120	26	2.3376794209775156
120	27	2.3783331284266893
120	28	1.6905146067327692
120	29	2.4600542880670204
120	30	2.5354547796228104
120	31	0.2464904613925185
120	32	-9.311818629838001
120	33	0.7831685642519162
120	34	0.7668592928708406
120	35	-0.5785237178039822
120	36	1.9870954216639234
120	37	1.9551113940138407
120	38	1.4412033185007758
120	39	2.401367697331068
120	40	6.403157170962547
120	41	-2.0052857788212997
120	42	-7.061550891011628
120	43	-11.997599050001895
120	44	-12.81538413907412
120	45	-10.40917193591547
120	46	-17.443038201525386
120	47	-16.224098682098884
120	48	-15.663356232194932
120	49	-18.98145555913517
120	50	-14.68532270354355
120	51	-13.601526631142901
120	52	-13.9450093279812
120	53	-13.30351727367285
120	54	-15.960829146708225
120	55	-13.515918164998048
120	56	-10.533563609374774
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	947.0
5	10.0
6	15.0
7	77.0
8	134.0
9	145.0
10	109.0
11	79.0
12	86.0
13	110.0
14	132.0
15	152.0
16	188.0
17	262.0
18	303.0
19	353.0
20	404.0
21	521.0
22	670.0
23	814.0
24	901.0
25	1004.0
26	1094.0
27	1222.0
28	1449.0
29	1658.0
30	1968.0
31	2499.0
32	2637.0
33	3065.0
34	3581.0
35	4378.0
36	5570.0
37	6853.0
38	6238.0
39	3708.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.34016908167283	0.543610699758187	0.09185146306259022	0.024368755506401486
8	98.80780549984067	0.9841228185277523	0.08622790409957448	0.12184377753200741
9	93.36420042364144	3.9402403134196864	1.6177104616941909	1.077848801244681
10	36.59812173130636	44.81414137627233	9.84310270493186	8.744634187489456
11	31.499428271505426	32.46668041314413	18.945770146399983	17.088121168950458
12	36.88679775807449	16.97752450934448	21.89813860198324	24.237539130597785
13	24.090602265056628	17.777319432985824	25.76314407860196	32.36893422335558
14	25.539892021595676	22.304289142171566	26.48095380923815	25.674865026994603
15	27.58892834996336	19.576451134036116	25.75492793656162	27.079692579438902
16	28.85329532240519	22.392062718031433	25.569226152518848	23.185415807044528
17	29.28374604007723	23.6114495660487	22.41363150692635	24.691172886947722
18	26.296747586465465	25.055769050520198	22.766894741775236	25.880588621239102
19	25.64309435803141	27.885246824399125	22.761131020507722	23.710527797061747
20	26.205084904775305	26.475279106858054	23.521906370203585	23.797729618163054
21	26.417605488593548	22.947869608412844	25.52345961347405	25.111065289519562
22	27.909723133447056	23.652688998444148	23.675183234296213	24.762404633812586
23	26.71565411363338	25.21791290981686	25.118563368136915	22.947869608412844
24	26.90173583773854	24.76286881865557	24.97281895549807	23.362576388107826
25	25.53941325335083	25.02015184178461	27.325897459930644	22.11453744493392
26	24.526179629942074	22.68338863580976	31.273081753932097	21.517349980316066
27	24.383700423999098	20.535814791189825	32.53536452665941	22.545120258151663
28	23.373308559953458	22.356098568023572	34.037122534391834	20.23347033763114
29	22.597884788478847	22.504125412541253	34.98724872487249	19.91074107410741
30	20.64569350181859	21.723724174134762	38.26727661329634	19.363305710750307
31	19.99737493437336	22.10117752943824	40.01350033750844	17.887947198679967
32	20.49463334083915	20.5753208736771	41.70419575170757	17.225850033776176
33	19.15660614708699	21.225662304500478	43.40887328439196	16.208858264020577
34	17.69317329332333	20.913353338334584	45.62828207051763	15.765191297824458
35	17.108027006751687	22.357464366091524	46.009002250562645	14.52550637659415
36	17.537642271849393	22.11846768296113	44.85008719131462	15.493802853874858
37	18.507737379807693	21.991436298076923	45.04957932692308	14.451246995192307
38	17.716165413533837	22.169172932330827	45.765037593984964	14.349624060150376
39	18.250986285928988	21.865489385684764	44.45989103888785	15.423633289498403
40	14.087698757412092	17.60584658382747	40.20848283415795	28.097971824602485
41	11.960799152414106	16.414408960193732	38.12055395792341	33.504237929468744
42	16.00420570398603	21.890313738007173	45.731398208820714	16.374082349186082
43	14.665639213622525	18.91891891891892	45.000563846182764	21.414878021275797
44	15.054735929757127	19.10199159648032	44.38603433007367	21.45723814368888
45	16.37863019874516	21.86948191005748	46.10399368824435	15.647894202953
46	16.413705011646254	22.2931850627395	45.48238034412803	15.810729581486212
47	15.574308485700891	21.393342709798404	45.755274261603375	17.277074542897328
48	17.236847046453114	22.460475421532916	45.81095797814413	14.491719553869842
49	13.70575596517019	19.578574390289873	40.85152097704399	25.864148667495947
50	15.770703506269241	23.329454163225467	45.51955852541482	15.380283805090473
51	16.085967320384334	23.69178120828084	44.75490288250005	15.467348588834778
52	15.917578396231727	24.735864282095072	43.90939628802522	15.43716103364798
53	16.001502488496573	24.10930603812565	44.464268945440885	15.424922527936896
54	14.079503806033266	23.845503242176488	41.90771544027817	20.167277511512076
55	16.069314187982783	24.374612363034938	44.02616197116921	15.52991147781307
56	16.40711588676197	26.098472751864442	42.58824413427761	14.906167227095974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	18.5
9	35.0
10	61.5
11	88.0
12	146.0
13	203.5
14	203.0
15	235.0
16	267.0
17	367.0
18	467.0
19	612.5
20	758.0
21	842.0
22	1089.5
23	1253.0
24	1579.5
25	1906.0
26	2246.5
27	2587.0
28	2821.5
29	3095.0
30	3134.0
31	2966.5
32	2799.0
33	2561.5
34	2324.0
35	1965.0
36	1606.0
37	1387.5
38	1035.5
39	902.0
40	889.0
41	876.0
42	943.5
43	1011.0
44	1002.5
45	994.0
46	1007.0
47	1124.5
48	1229.0
49	1319.5
50	1410.0
51	1485.5
52	1561.0
53	1713.0
54	1879.5
55	1894.0
56	2034.5
57	2175.0
58	2313.0
59	2451.0
60	2610.0
61	2769.0
62	2630.0
63	2252.0
64	2013.0
65	1837.5
66	1662.0
67	1469.0
68	1276.0
69	1098.0
70	920.0
71	831.5
72	653.0
73	563.0
74	472.0
75	381.0
76	282.5
77	184.0
78	147.0
79	77.0
80	44.0
81	27.5
82	11.0
83	7.0
84	3.0
85	3.0
86	3.0
87	2.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.028117794815078638
14	0.005623558963015727
15	0.24368755506401482
16	0.054361069975818695
17	0.0
18	0.0037490393086771516
19	0.0937259827169288
20	0.09747502202560594
21	0.0
22	0.0
23	0.0
24	0.0018745196543385758
25	0.0037490393086771516
26	0.007498078617354303
27	0.08435338444523591
28	0.11996925787766885
29	0.03561587343243294
30	0.01687067688904718
31	0.028117794815078638
32	0.10309858098862165
33	0.16120869027311752
34	0.050612030667141546
35	0.050612030667141546
36	0.029992314469417213
37	0.18557744577951898
38	0.27555438918777064
39	0.21931879955761338
40	0.7366862241550602
41	0.9203891502802406
42	0.16120869027311752
43	0.2643072712617392
44	0.5136183852887698
45	0.21182072094025908
46	0.2080716816315819
47	0.041239432395448666
48	0.16683224923613324
49	0.5417361801038484
50	0.13309089545803887
51	0.30742122331152644
52	0.11247117926031455
53	0.19120100474253474
54	0.26618179091607774
55	0.2624327516074006
56	0.21369524059459763
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	53347.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.36704969351604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.79039565817847	67.14716853806212
2	7.738715382937998	11.974431551914822
3	2.6336830373367577	6.112808592798095
4	1.056380684709132	3.269162277166476
5	0.5209216679184939	2.015108628413969
6	0.3367819155380031	1.5633493917183723
7	0.27378673709204565	1.4827450465818135
8	0.13568192280667749	0.8397848051436819
9	0.10418433358369877	0.7254391062290289
>10	0.40704576842003243	4.736911166513581
>50	0.0024228914786906695	0.13309089545803887
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71	0.13309089545803887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0018745196543385758	0.0	0.0	0.0	0.0
8	0.0018745196543385758	0.0	0.0	0.0	0.0
9	0.0018745196543385758	0.0	0.0	0.0	0.0
10	0.005623558963015727	0.0	0.0	0.0	0.0
11	0.00937259827169288	0.0	0.0	0.0	0.0
12	0.01312163758037003	0.0	0.0	0.0	0.0
13	0.01687067688904718	0.0	0.0	0.0	0.0
14	0.01687067688904718	0.0	0.0	0.0	0.0
15	0.022494235852062908	0.0	0.0	0.0	0.0
16	0.02624327516074006	0.0	0.0	0.0	0.0
17	0.029992314469417213	0.0	0.0	0.0	0.0
18	0.035615873432432936	0.0	0.0	0.0	0.0
19	0.03936491274111009	0.0	0.0	0.0	0.0
20	0.0468629913584644	0.0	0.0	0.0	0.0
21	0.050612030667141546	0.0	0.0	0.0	0.0
22	0.059984628938834425	0.0	0.0	0.0	0.0
23	0.07123174686486587	0.0	0.0	0.0	0.0
24	0.08247886479089733	0.0	0.0	0.0	0.0
25	0.08997694340825163	0.0	0.0	0.0	0.0
26	0.09747502202560594	0.0	0.0	0.0	0.0
27	0.10872213995163739	0.0	0.0	0.0	0.0
28	0.12934185614936172	0.0	0.0	0.0	0.0
29	0.13683993476671602	0.0	0.0	0.0	0.0
30	0.14246349372973174	0.0	0.0	0.0	0.0
31	0.14621253303840892	0.0	0.0	0.0	0.0
32	0.14621253303840892	0.0	0.0	0.0	0.0
33	0.15183609200142464	0.0	0.0	0.0	0.0
34	0.15371061165576322	0.0	0.0	0.0	0.0
35	0.1555851313101018	0.0	0.0	0.0	0.0
36	0.15745965096444037	0.0	0.0	0.0	0.0
37	0.16495772958179467	0.0	0.0	0.0	0.0
38	0.1705812885448104	0.0	0.0	0.0	0.0
39	0.17245580819914896	0.0	0.0	0.0	0.0
40	0.1762048475078261	0.0	0.0	0.0	0.0
41	0.1837029261251804	0.0	0.0	0.0	0.0
42	0.19120100474253474	0.0	0.0	0.0	0.0
43	0.19869908335988903	0.0	0.0	0.0	0.0
44	0.20432264232290476	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCAGG	15	0.0081613725	50.34568	46
ATGTCAG	15	0.0081613725	50.34568	45
TTGTTTC	15	0.008192093	50.297913	35
CCAGTAC	20	4.0275764E-4	50.297913	35
AGTACCA	20	4.0275764E-4	50.297913	37
TTGATAT	15	0.008192093	50.297913	49
CCCAGTA	20	4.046501E-4	50.250237	34
ACATGTC	15	0.008222899	50.250237	43
CCAAAAC	30	9.939886E-7	50.250237	43
CGACTCC	20	4.046501E-4	50.250237	44
CATGTCA	15	0.008222899	50.250237	44
CAAAAAC	15	0.008222899	50.250237	43
GCTCAAG	15	0.008315837	50.10775	42
AACATGT	15	0.008315837	50.10775	42
TAAAAAG	15	0.00834699	50.060432	14
CTTAATC	25	2.045688E-5	50.060432	15
TCTTAAT	25	2.045688E-5	50.060432	14
TGATATG	15	0.008378228	50.013206	50
CTCATGA	15	0.008378228	50.013206	32
TCTCATG	15	0.008378228	50.013206	31
>>END_MODULE