FastQCFastQC Report
Fri 17 Jun 2016
SRR1526270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29276
Sequences flagged as poor quality0
Sequence length56
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT710.24251946987293346No Hit
TCTACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.18103566060937287No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG530.18103566060937287No Hit
TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC460.15712529034021042No Hit
TCTACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAACAAAAAAAAAAAAAAA390.13321492007104796No Hit
TCTACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.12296761852712118No Hit
TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC330.11272031698319443No Hit
TCTACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.10588878262057658No Hit
TCTACCGGGAAGCAACGCAGAGTCGACTTTTCTTTTTTTTCTTTTTTCTTTTTTTT300.10247301543926765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA150.00810263450.37996737
ATATGCT150.00810263450.37996734
ACAACCT150.00810263450.37996740
CATATGC204.102713E-450.03430633
CAGCTAG150.00832673150.034333
TAGTGAG150.00832673150.034347
CCTGCCT150.00832673150.034344
CCAAAAC150.00832673150.034343
GTGAGTC150.00832673150.034349
TGCCTGC150.00832673150.034346
GTGGGTT150.00832673150.034342
ATAGTGA150.00832673150.034346
AGTGAGT150.00832673150.034348
CAGATTT150.00832673150.034349
TGGGTTT150.00832673150.034343
GCCTGCA150.00832673150.034347
TGAGTCC150.00832673150.034350
ATCCTGC301.0200947E-649.9486320
TCCTTCG150.00838346849.9486316
TCTAGGC150.00838346849.9486326