##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526270_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29276 Sequences flagged as poor quality 0 Sequence length 56 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.088160950949586 39.0 38.0 39.0 35.0 40.0 2 37.70822516737259 39.0 38.0 39.0 35.0 40.0 3 37.64633146604727 39.0 38.0 39.0 35.0 40.0 4 36.07132121874573 39.0 37.0 39.0 27.0 40.0 5 37.7797513321492 39.0 38.0 39.0 35.0 40.0 6 37.84164503347452 39.0 38.0 39.0 35.0 40.0 7 37.65295805437901 39.0 38.0 39.0 35.0 40.0 8 37.6388167782484 39.0 38.0 39.0 35.0 40.0 9 37.54621532996311 39.0 38.0 39.0 35.0 40.0 10 37.080885366853394 39.0 38.0 39.0 33.0 40.0 11 36.08734116682607 38.0 35.0 39.0 30.0 40.0 12 34.129252630140726 38.0 33.0 39.0 22.0 40.0 13 33.745115452930726 38.0 33.0 39.0 21.0 40.0 14 33.91638201940156 38.0 33.0 39.0 21.0 40.0 15 33.7411190053286 38.0 33.0 39.0 20.0 40.0 16 33.01041808990299 37.0 31.0 38.0 20.0 40.0 17 32.948012023500475 36.0 30.0 38.0 20.0 40.0 18 32.27466183904905 36.0 29.0 38.0 17.0 40.0 19 33.25700232272168 36.0 31.0 38.0 22.0 40.0 20 33.41156578767591 36.0 31.0 38.0 23.0 40.0 21 34.29379013526438 38.0 33.0 39.0 24.0 40.0 22 33.941453750512366 38.0 33.0 39.0 23.0 40.0 23 34.62805711162727 38.0 33.0 39.0 26.0 40.0 24 34.205150976909415 38.0 33.0 39.0 23.0 40.0 25 34.55065582729881 38.0 33.0 39.0 26.0 40.0 26 35.450437218199205 38.0 35.0 39.0 29.0 40.0 27 35.42765405109988 38.0 35.0 39.0 28.0 40.0 28 35.46956551441454 38.0 35.0 39.0 29.0 40.0 29 35.203374777975135 38.0 35.0 39.0 27.0 40.0 30 35.20986473561962 38.0 35.0 39.0 28.0 40.0 31 35.758915152343214 38.0 35.0 39.0 30.0 40.0 32 35.43428063943162 38.0 35.0 39.0 29.0 40.0 33 35.62949173384342 38.0 35.0 39.0 30.0 40.0 34 35.68745730291023 38.0 35.0 39.0 30.0 40.0 35 35.52356879355103 38.0 35.0 39.0 29.0 40.0 36 34.80482306326001 38.0 35.0 39.0 29.0 40.0 37 34.55191966115589 38.0 35.0 39.0 28.0 40.0 38 34.267078835906545 38.0 34.0 39.0 27.0 40.0 39 33.72421095778112 37.0 33.0 39.0 24.0 40.0 40 30.434861319852438 35.0 26.0 39.0 10.0 39.0 41 26.901933324224622 32.0 15.0 38.0 5.0 39.0 42 31.41686022680694 35.0 30.0 38.0 15.0 39.0 43 30.09721273398005 35.0 28.0 38.0 7.0 39.0 44 29.816539144691898 35.0 27.0 38.0 5.0 39.0 45 30.99422735346359 35.0 30.0 38.0 4.0 39.0 46 30.595163273671268 35.0 29.0 38.0 4.0 39.0 47 30.08102199754065 35.0 28.0 38.0 4.0 39.0 48 30.214885913376143 35.0 28.0 38.0 4.0 39.0 49 27.53395272578221 35.0 17.0 38.0 4.0 39.0 50 29.715329963109713 35.0 27.0 38.0 4.0 39.0 51 29.801919661155896 35.0 28.0 39.0 4.0 40.0 52 29.669524525208363 35.0 28.0 39.0 4.0 40.0 53 29.2818349501298 35.0 26.0 39.0 4.0 40.0 54 28.21109441180489 35.0 18.0 39.0 4.0 40.0 55 28.432367809810085 35.0 23.0 39.0 4.0 40.0 56 26.98254542970351 34.0 17.0 39.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.4432518558096774 1 2 0.9272652643222088 1 3 1.268739103119799 1 4 2.9149629613208745 1 5 0.9592816472306112 1 6 0.8501880958718218 1 7 1.008035054887138 1 8 0.8733127300425494 1 9 1.1345813318002556 1 10 1.1771377478462028 1 11 -2.616600014644469 1 12 -14.915327109246785 1 13 -7.016636610350947 1 14 -6.723495008809049 1 15 -8.022018003043126 1 16 -22.987504780435344 1 17 -5.2666275413915535 1 18 -15.673189424257236 1 19 -5.994637618993412 1 20 -6.2322616803002475 1 21 -7.589582269030817 1 22 -5.095156009056073 1 23 -5.660406989183137 1 24 -6.056207795228481 1 25 -5.389639251009022 1 26 2.30278646324615 1 27 2.3650595771470435 1 28 1.718432452001629 1 29 2.2688242587429244 1 30 2.475081332991728 1 31 2.051537438804097 1 32 1.9040713559476927 1 33 2.297336845213323 1 34 1.5126258381784083 1 35 2.5485178414889305 1 36 2.6196083290611654 1 37 3.1336697350974703 1 38 2.3074510243861823 1 39 3.2931347708683845 1 40 6.59327464086817 1 41 -9.288173512587669 1 42 -8.583660117219484 1 43 -17.1338969350653 1 44 -22.233622093426888 1 45 -10.275525541569149 1 46 -15.88524012667764 1 47 -23.766177737367872 1 48 -12.892605387110965 1 49 -20.359602453254617 1 50 -12.909800229466214 1 51 -8.946870733287579 1 52 -8.75566970323899 1 53 -9.766841920000719 1 54 -8.415985874918867 1 55 -9.021488454068638 1 56 -3.358573596763197 2 1 0.4229815855394037 2 2 1.0901031021600502 2 3 1.1694147787954776 2 4 2.333881880239794 2 5 1.034957322906287 2 6 0.7508637715475004 2 7 0.8107377575898411 2 8 0.963177594907414 2 9 1.2163380885570163 2 10 -0.010700089991637185 2 11 -0.13349190653636356 2 12 -6.160597379517057 2 13 -3.670690664405001 2 14 0.415018504704463 2 15 0.3050090239838994 2 16 0.24019792226735603 2 17 1.347561647797633 2 18 -4.554946181013992 2 19 0.6161731918173956 2 20 0.3454410224024542 2 21 0.20568800123945152 2 22 1.2007899368898762 2 23 0.7983767946006424 2 24 0.12757598855530006 2 25 0.33198237061260016 2 26 0.6737324091920911 2 27 1.9360055230929873 2 28 1.0008648844340655 2 29 1.0661215560402226 2 30 1.7440002519106486 2 31 -0.27481391254725906 2 32 0.9182605451368815 2 33 1.2459854938619657 2 34 0.6869501625027326 2 35 0.45797730094839295 2 36 1.4986623831152244 2 37 0.9336697350974674 2 38 0.45947805141320686 2 39 1.1661077438413585 2 40 4.319625992219525 2 41 -2.85641675583091 2 42 -6.260687144246511 2 43 -8.610923962092329 2 44 -15.019432904237698 2 45 -4.796471487515095 2 46 -10.310240126677641 2 47 -19.6459074670976 2 48 -8.014227008732586 2 49 -13.827845696497858 2 50 -7.509800229466215 2 51 -1.8157896522064974 2 52 -1.7016156491849372 2 53 -1.8844094875682877 2 54 -0.7436885776215689 2 55 -1.5228398054199879 2 56 3.3481831599935603 3 1 1.2949328050516016 3 2 1.8138835899649237 3 3 2.0907562422101122 3 4 3.6143696851178433 3 5 1.7569085424184863 3 6 1.5014735276450608 3 7 1.5400060502727655 3 8 1.665006863200098 3 9 1.7693868690448156 3 10 1.9978364953742158 3 11 2.711020288585587 3 12 4.765622132678068 3 13 3.1732117746193893 3 14 2.204652651045926 3 15 2.980618780081464 3 16 4.203002800316135 3 17 4.727439696578124 3 18 4.618834306790884 3 19 4.47470977718325 3 20 4.348489802890256 3 21 4.0465416597760395 3 22 3.7044484734752388 3 23 3.5624011848445463 3 24 3.8739174519699375 3 25 3.695397004758938 3 26 3.248732409192094 3 27 3.140273815775913 3 28 3.5167185429706507 3 29 2.883194726771933 3 30 3.394000251910647 3 31 3.1148202337942053 3 32 2.8981385939173663 3 33 2.872814762154654 3 34 2.5930477234783424 3 35 2.6177333985093654 3 36 4.088296529456684 3 37 3.401962418024297 3 38 4.553990246535157 3 39 5.314888231646236 3 40 8.700723553195136 3 41 10.736875927095916 3 42 6.6088250508754385 3 43 2.717124818395476 3 44 4.833615876250104 3 45 7.315113878338565 3 46 3.578174507468699 3 47 -0.5934684427073584 3 48 3.827236405901562 3 49 5.86056893764848 3 50 3.9682485510215884 3 51 4.451283518525212 3 52 4.664238009351649 3 53 4.982663683163423 3 54 6.295945568719894 3 55 5.627160194580011 3 56 7.6176953551155115 4 1 0.5832379957958125 4 2 1.585615922672865 4 3 1.6623634967441916 4 4 3.1672152135731295 4 5 1.1734188613678285 4 6 1.03868428436801 4 7 1.1722762191282996 4 8 1.5843314410612592 4 9 1.634927832146758 4 10 0.18545375616221094 4 11 1.6966362985918408 4 12 4.173376979457302 4 13 4.3652067714924385 4 14 3.225915940601901 4 15 3.5178295368044132 4 16 4.253659460728898 4 17 3.896920622156607 4 18 5.288002536934723 4 19 3.9443783200225297 4 20 3.0089025608639943 4 21 2.4499187704702194 4 22 3.978995065095006 4 23 2.7644024356262875 4 24 3.496806757786068 4 25 2.5653157039459344 4 26 2.1718093322690137 4 27 2.234082446169907 4 28 -0.028622295053118307 4 29 2.258429248347916 4 30 2.6760515339619317 4 31 1.8283912156578737 4 32 1.8624913143676451 4 33 1.253677801554275 4 34 1.9395142650668333 4 35 2.366951659922748 4 36 3.5198162292690682 4 37 3.467003068430806 4 38 3.5396062565414113 4 39 3.34303082076444 4 40 6.431805479399014 4 41 9.186531962117805 4 42 4.799569266009897 4 43 6.480101678933313 4 44 6.160695300890506 4 45 5.265707999664393 4 46 5.781426539989024 4 47 4.965630994440858 4 48 4.870388375882801 4 49 6.80741071375855 4 50 3.7055843859183994 4 51 3.4162616298447865 4 52 2.2983843508150628 4 53 3.5117443585855597 4 54 4.958234499301511 4 55 3.781006348426164 4 56 5.244337006147408 5 1 1.1242010977345274 5 2 1.6187616387454113 5 3 1.8224635592832783 5 4 3.6875404168251578 5 5 1.561786591198974 5 6 1.379522308132863 5 7 1.02781092832155 5 8 1.372323936370826 5 9 1.3791429666057908 5 10 1.3392999100083642 5 11 1.9305324837075375 5 12 4.838792864385383 5 13 4.417114213643778 5 14 4.082701431533728 5 15 4.7611065849595136 5 16 5.568856458852721 5 17 4.971342135602512 5 18 5.252980648254297 5 19 4.596660996695448 5 20 3.0558068760609913 5 21 3.94898068416628 5 22 3.997131400304511 5 23 2.9038645994786947 5 24 4.361722330018715 5 25 2.305153102319913 5 26 2.858488506753069 5 27 2.3110055230929873 5 28 2.150864884434064 5 29 2.883194726771933 5 30 3.1744880567886966 5 31 2.2855519411112795 5 32 2.581065423185656 5 33 2.5313513475205056 5 34 2.129633089331996 5 35 2.8128553497288777 5 36 3.2102477489688823 5 37 3.7190355887560074 5 38 3.627160978242472 5 39 3.558790670670625 5 40 8.115357699536599 5 41 10.858827146608114 5 42 6.291751880143735 5 43 7.1561492086393805 5 44 6.809225632347665 5 45 6.217552902728805 5 46 4.358662312346748 5 47 5.162629118268249 5 48 4.607724210779612 5 49 6.128861620575314 5 50 3.4316631851679347 5 51 2.3293322990130143 5 52 2.2983843508150628 5 53 2.177785634382932 5 54 2.198384593110138 5 55 2.0174040970190354 5 56 2.495744135603317 6 1 1.1242010977345274 6 2 1.3748591997210227 6 3 1.6761220958686494 6 4 3.272906270483695 6 5 1.147152444857511 6 6 1.1356198691084742 6 7 0.6863475136874015 6 8 1.079641009541561 6 9 1.2571917470936 6 10 1.0953974709839756 6 11 1.8817519959026612 6 12 4.3997684741414815 6 13 4.4902849453511 6 14 4.180262407143488 6 15 3.4928139020326796 6 16 4.59324670275516 6 17 4.825000672187883 6 18 5.155419672644545 6 19 4.499100021085688 6 20 4.177758095573189 6 21 3.680688001239453 6 22 3.850789936889875 6 23 2.6111816726494226 6 24 3.8495272080674994 6 25 3.1588116389052843 6 26 1.7121470433384331 6 27 2.0427128401661605 6 28 1.5167185429706507 6 29 2.1027069218938834 6 30 2.076927081178937 6 31 2.1148202337942053 6 32 1.6542361548929776 6 33 0.9703757377644067 6 34 1.4223160161612682 6 35 1.5201724228996127 6 36 1.6736623831152215 6 37 1.4995233936340568 6 38 1.9930146367790584 6 39 2.729522377987699 6 40 6.286089406853673 6 41 3.1271198295349443 6 42 4.0478494411193395 6 43 6.034197989127183 6 44 5.1019085591769375 6 45 4.632187049070268 6 46 4.748906214785773 6 47 5.943116923146299 6 48 5.193090064438149 6 49 8.397154303502141 6 50 4.017029038826472 6 51 4.47567376242765 6 52 5.371555082522377 6 53 4.494858805114639 6 54 5.905701666280869 6 55 6.139355316531233 6 56 6.056719745359416 7 1 0.6275270400848569 7 2 1.5378303748873208 7 3 1.376232960613656 7 4 3.3793364256943406 7 5 1.1349573229062884 7 6 1.1849546806384055 7 7 1.3016468484989332 7 8 1.3063594130892326 7 9 1.2549744521933803 7 10 1.3165726372810909 7 11 2.218780820736363 7 12 4.246220802301124 7 13 4.224763881049547 7 14 3.7672912319771896 7 15 3.5959181148929957 7 16 5.056107013176451 7 17 5.172561647797636 7 18 4.479144728076914 7 19 3.907082282726492 7 20 4.288622840584274 7 21 2.362506183057633 7 22 3.100789936889875 7 23 2.1461040673279186 7 24 3.386666897646208 7 25 3.7319823706125987 7 26 2.635096045555727 7 27 3.288278250365714 7 28 2.5372285207976972 7 29 2.975212465131129 7 30 2.825818433728827 7 31 2.4501860874527424 7 32 2.684169636045972 7 33 2.673258221134695 7 34 2.8596774352300045 7 35 1.75797730094839 7 36 1.9918442012970416 7 37 1.8836697350974703 7 38 2.334478051413207 7 39 3.33428956202318 7 40 6.262807810401345 7 41 -0.5086894831036375 7 42 3.071131037571668 7 43 4.111803310634944 7 44 4.319203459398668 7 45 3.5512557852121773 7 46 4.36475987332236 7 47 3.822274351084218 7 48 4.008500263994687 7 49 6.3516997580475945 7 50 4.61292704326106 7 51 3.884210347793502 7 52 4.071111623542336 7 53 4.27922687606808 7 54 5.358584149651158 7 55 3.990796558216374 7 56 4.034546796357198 8 1 0.9376874678923457 8 2 -0.7054851331340686 8 3 0.8855912493837081 8 4 2.12799952729862 8 5 -0.01210150062312465 8 6 0.14351083037102796 8 7 0.3577965811192527 8 8 0.41464818314270957 8 9 0.03839691208642648 8 10 -0.24893538410928073 8 11 0.6973904464048104 8 12 3.7261673263652995 8 13 3.4587211003008846 8 14 3.437077328233876 8 15 3.328538435748605 8 16 4.8916685105026545 8 17 4.775502824268223 8 18 4.8935832307507106 8 19 2.9044084859350434 8 20 2.823382198873041 8 21 1.915982118886511 8 22 2.586084054536933 8 23 1.9469062063653482 8 24 1.4628701062023595 8 25 1.6437470764949538 8 26 -0.22185582610202204 8 27 0.6933584642694584 8 28 0.3861590020811221 8 29 1.3602392030990487 8 30 -0.1354115127952369 8 31 0.20018608745274236 8 32 -0.5243865136866503 8 33 -0.5540145061380315 8 34 -0.4865792492619718 8 35 -0.46261093434572587 8 36 -0.9439846757083075 8 37 -0.04280085313782678 8 38 0.5403604043543808 8 39 -0.4103628443939371 8 40 3.0034495216312926 8 41 -1.0314167558309109 8 42 2.036371679282901 8 43 2.9861348614370833 8 44 3.1026259192917145 8 45 1.984410865426078 8 46 3.3794657556753016 8 47 3.2393866505494557 8 48 3.77989063832624 8 49 6.397154303502141 8 50 2.8254938881808442 8 51 3.163622112499386 8 52 1.9454431743444758 8 53 1.636178747725829 8 54 0.7048408341431376 8 55 0.4506896063447172 8 56 2.045241983522974 9 1 0.5847462914217587 9 2 1.4121619256894604 9 3 1.5032383082072442 9 4 3.098587762592736 9 5 1.3996632052592304 9 6 1.349393183312202 9 7 0.7695612870016078 9 8 1.0617070066721155 9 9 1.3913380885570135 9 10 0.13341755706719027 9 11 1.9620963287577524 9 12 4.432049679306473 9 13 3.8116622767714716 9 14 4.201783210586818 9 15 3.446185494572134 9 16 4.8916685105026545 9 17 4.481385177209404 9 18 5.158289113103653 9 19 4.933820250640927 9 20 4.499852787108338 9 21 2.798335060062982 9 22 3.527260525125172 9 23 2.21161208871829 9 24 3.3452230473788305 9 25 3.526100017671425 9 26 2.71932064448621 9 27 3.0462996407400453 9 28 1.092041355022296 9 29 2.2720039089813966 9 30 1.6292943695577051 9 31 1.2295978521586264 9 32 0.9167899569015816 9 33 1.7695149056266715 9 34 1.1310678095615572 9 35 0.3903302421248611 9 36 1.4971917948799245 9 37 0.8395520880386442 9 38 1.2168309925896779 9 39 1.560225390900186 9 40 3.8858024628077565 9 41 -0.5902402852426754 9 42 0.418724620459372 9 43 1.8978995673194348 9 44 3.9555670957623015 9 45 0.896175571308433 9 46 2.4382892850870626 9 47 2.0040925329023977 9 48 0.8681259324438848 9 49 3.161860185855083 9 50 0.060788005827902225 9 51 0.9283279948523244 9 52 1.6807372919915338 9 53 1.547943453608184 9 54 3.2930761282607826 9 55 1.1859837239917752 9 56 2.868771395287677 10 1 1.1189275314853475 10 2 1.602940939997886 10 3 1.4849553193360165 10 4 3.56361160996952 10 5 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1.0450143379854282 114 36 1.6366253460781834 114 37 2.2447808462085845 114 38 2.0381817551169092 114 39 3.1920336697672838 114 40 6.203885251478784 114 41 7.098212873798715 114 42 2.833757300197931 114 43 -0.3461091472775166 114 44 -7.89628475608955 114 45 1.297047031003423 114 46 -4.959314200751713 114 47 -3.681092652282789 114 48 -4.514227008732586 114 49 -17.676919770571935 114 50 -3.8727631924291757 114 51 -5.347271133687979 114 52 -5.479393426962716 114 53 -6.334409487568287 114 54 -11.414984873917865 114 55 -6.261728694308879 114 56 -1.5277427659323664 115 1 0.03505055105664212 115 2 0.6961375849186666 115 3 0.8754492615540954 115 4 2.764916362998413 115 5 0.5659918056649076 115 6 0.4568982543061182 115 7 0.5728067231070781 115 8 0.6692120776660317 115 9 0.6844415368328782 115 10 0.5461964617324995 115 11 -2.8533194927432604 115 12 -10.392493931241194 115 13 -7.277587216129138 115 14 -8.623774598743815 115 15 -11.477749596705753 115 16 -9.767560698422297 115 17 -3.560196972892019 115 18 -5.774773767220889 115 19 -3.9769302564584628 115 20 -5.495076218976855 115 21 1.6462052426187626 115 22 1.0921692472347004 115 23 1.9854457601178837 115 24 1.2448173678656431 115 25 1.6802582326815667 115 26 0.8694220643645068 115 27 0.828246902403329 115 28 0.7370717809857865 115 29 1.048880176729881 115 30 1.1526209415658215 115 31 0.217427466763084 115 32 -0.423980834173463 115 33 -0.6402214026897539 115 34 -0.8182222512903721 115 35 -0.3023675266378163 115 36 -1.1194410651606432 115 37 -0.21115785110942653 115 38 -0.7344874658281739 115 39 0.7488663645310183 115 40 4.013591509460905 115 41 4.054790140720812 115 42 -0.6451699028672024 115 43 -6.5057515482992265 115 44 -8.492708766306663 115 45 -1.4628507978599252 115 46 -4.712826333574192 115 47 -4.0131488464079474 115 48 -3.341813215629138 115 49 -16.844225006842684 115 50 -4.806351953604146 115 51 -4.279582755654776 115 52 -6.184374269874592 115 53 -6.024064659982081 115 54 -12.118688577621569 115 55 -4.786632908868267 115 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-5.703472021075807 117 21 2.1589488708046716 117 22 2.1116595021072655 117 23 1.0798985337310825 117 24 1.3362716407292154 117 25 1.5363301966995522 117 26 2.1182976265833986 117 27 2.571875088310378 117 28 1.4986909713905874 117 29 2.087860686475004 117 30 1.8722611214758658 117 31 0.04801217440926564 117 32 0.5715214147020973 117 33 0.07642027647066385 117 34 0.18477624945925442 117 35 0.0949338226875227 117 36 1.2823580352891355 117 37 1.8728001698800796 117 38 1.1605650079349488 117 39 2.831325135145711 117 40 6.509843383523876 117 41 4.9033658528647415 117 42 -0.30633931815955506 117 43 -9.987010918614068 117 44 -11.21834594771596 117 45 2.3002676429196853 117 46 -1.754805344068945 117 47 -2.6263422497062976 117 48 -2.1229226609065 117 49 -11.733280479106554 117 50 -1.648930664248823 117 51 -1.4310070435108457 117 52 -2.310311301358851 117 53 -0.943105139742201 117 54 -5.37955814283896 117 55 -1.155448501072165 117 56 -0.6452951008760053 118 1 -0.4936850811272606 118 2 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22 2.4907899368898754 119 23 1.7633767946006458 119 24 2.407575988555301 119 25 1.8319823706126002 119 26 1.5687324091920942 119 27 2.3910055230929856 119 28 1.630864884434061 119 29 2.0061215560402204 119 30 2.4740002519106454 119 31 1.2201860874527455 119 32 -2.4667394548631236 119 33 1.065985493861966 119 34 0.35695016250273426 119 35 0.20797730094839295 119 36 0.11366238311521926 119 37 0.4936697350974697 119 38 1.1744780514132103 119 39 1.6661077438413585 119 40 5.704625992219523 119 41 0.9085832441690904 119 42 -4.170687144246511 119 43 -12.400923962092328 119 44 -12.444432904237697 119 45 -10.901471487515096 119 46 -18.40524012667764 119 47 -14.215907467097603 119 48 -16.354227008732586 119 49 -14.842845696497859 119 50 -12.789800229466213 119 51 -13.545789652206498 119 52 -13.141615649184937 119 53 -13.854409487568287 119 54 -17.91868857762157 119 55 -12.972839805419989 119 56 -10.201816840006439 120 1 -0.8770184144605935 120 2 -0.3848968978399512 120 3 0.1944147787954762 120 4 1.5338818802397967 120 5 -0.21504267709371305 120 6 -0.3741362284524996 120 7 -0.0392622424101603 120 8 0.038177594907409684 120 9 0.14133808855701346 120 10 -0.7607000899916372 120 11 -2.2584919065363636 120 12 -12.035597379517057 120 13 -5.170690664405001 120 14 -12.059981495295538 120 15 -9.944990976016097 120 16 -15.734802077732642 120 17 -10.527438352202363 120 18 -16.37994618101399 120 19 -8.433826808182602 120 20 -11.779558977597546 120 21 -6.869311998760548 120 22 -6.0992100631101245 120 23 -3.626623205399355 120 24 -10.5724240114447 120 25 -4.818017629387402 120 26 1.4487324091920968 120 27 0.061005523092987346 120 28 0.6508648844340641 120 29 -0.23387844395977453 120 30 -0.10599974808935286 120 31 -0.5998139125472548 120 32 -7.456739454863122 120 33 0.39598549386197135 120 34 0.286950162502734 120 35 -1.1920226990516127 120 36 1.523662383115223 120 37 0.2336697350974717 120 38 1.0844780514132069 120 39 2.76610774384136 120 40 5.394625992219527 120 41 -2.1814167558309094 120 42 -7.810687144246511 120 43 -11.71092396209233 120 44 -9.3444329042377 120 45 -8.721471487515096 120 46 -14.435240126677641 120 47 -12.6459074670976 120 48 -12.414227008732585 120 49 -19.002845696497857 120 50 -11.809800229466212 120 51 -13.265789652206498 120 52 -13.251615649184938 120 53 -12.734409487568287 120 54 -16.41868857762157 120 55 -12.62283980541999 120 56 -9.101816840006439 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 141.0 5 17.0 6 14.0 7 34.0 8 57.0 9 52.0 10 38.0 11 37.0 12 24.0 13 21.0 14 40.0 15 61.0 16 100.0 17 107.0 18 108.0 19 161.0 20 186.0 21 256.0 22 300.0 23 353.0 24 432.0 25 500.0 26 517.0 27 627.0 28 756.0 29 924.0 30 1136.0 31 1329.0 32 1655.0 33 1809.0 34 2177.0 35 2462.0 36 3131.0 37 3983.0 38 3823.0 39 1904.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.159721273398 0.526028145921574 0.09564148107664981 0.218609099603771 8 98.47315206995492 1.2809126929908459 0.14687798879628364 0.09905724825795872 9 91.43667167645853 5.73507309741768 1.6429840142095915 1.185271211914196 10 24.13922667031015 51.912829621533 12.395819100970078 11.552124607186775 11 26.113540101106707 39.64680967345266 17.939609236234457 16.300040989206177 12 38.65282142369176 17.758573575625086 19.148790818417815 24.439814182265337 13 25.092238316479914 18.734626947253346 23.654003826182016 32.51913091008472 14 23.299743808710506 26.73953885567891 25.30145175064048 24.65926558497011 15 28.924488259053877 21.260354624495108 26.244266447593617 23.5708906688574 16 25.374307786969304 23.422437957202433 26.211800095713407 24.991454160114856 17 26.690804754747916 25.987156715398278 22.479163820194014 24.84287470965979 18 23.432162863779205 28.83249077742861 22.335701598579043 25.399644760213143 19 21.30671134055865 31.474580327532564 22.00759000307703 25.211118328831756 20 22.113215286798386 28.587543583783415 23.261776167361727 26.03746496205647 21 22.885640114769778 25.1912829621533 23.664435032108212 28.25864189096871 22 24.55595026642984 26.622489411121737 21.91897800245935 26.902582319989072 23 25.177619893428066 29.52247574805301 23.357015985790408 21.942888372728515 24 21.690121601311656 29.191146331466044 25.847110260964612 23.271621806257688 25 22.451837682743545 28.883727285148243 26.24675502117776 22.417680010930454 26 21.05622736899638 27.42365238778438 31.717565074810413 19.802555168408826 27 19.405791650996616 26.26756470306677 33.85414885979008 20.472494786146537 28 18.078303983586938 27.881689177637202 36.06428449307574 17.97572234570012 29 16.850017082336862 26.51178681243594 39.576358045780665 17.061838059446533 30 13.347226018037714 27.688576113692264 43.43058212626401 15.533615742006013 31 13.421259438958554 25.684217719615948 46.991492124235485 13.90303071719001 32 13.87340560134049 24.286153951372977 48.71593201791882 13.124508429367712 33 12.565856996236743 24.17721518987342 50.06158056790968 13.195347245980157 34 11.12895510148295 25.46982846989681 53.471605275746604 9.929611152873642 35 11.358277806253202 24.74970100803007 54.18076200239194 9.711259183324792 36 11.145658933269553 24.341408412204874 54.08822222981515 10.424710424710424 37 9.925413986588204 24.048857260161487 55.662378541124944 10.36335021212536 38 11.434931506849315 25.575342465753426 54.24657534246575 8.743150684931507 39 11.150660551714696 25.04278184680676 53.39516736258471 10.411390238893832 40 7.846577227382181 20.653594771241828 47.27554179566563 24.224286205710357 41 8.253213411902546 17.908956201109618 44.53633826113925 29.301492125848583 42 9.841671511130869 26.21482064083712 53.65728550422323 10.286222343808776 43 7.873476653430098 23.237025879775437 53.21785567574969 15.671641791044777 44 8.771749202100278 23.830605031058035 50.633172037475546 16.76447372936614 45 9.62012320328542 26.509240246406574 53.63107460643395 10.23956194387406 46 9.661190965092402 27.347707049965774 53.26488706365503 9.72621492128679 47 10.232669377156718 26.09245276572483 52.78280774881273 10.892070108305715 48 9.991106854562867 28.10576002189082 52.76029552606376 9.142837597482556 49 8.291293455506366 23.408490339407667 46.82041250557672 21.479803699509247 50 9.706546275395034 28.69895341678637 52.78404815650865 8.810452151309939 51 10.674888660500171 29.167523124357658 51.14080164439877 9.016786570743406 52 10.189143893012279 29.05564866436365 50.986763347812705 9.76844409481137 53 9.011289770783442 30.8518645227506 51.35135135135135 8.785494355114608 54 9.483053748716193 29.147552208147893 47.81923998630606 13.550154056829852 55 10.438206093803492 30.023964395754877 50.79767203012667 8.740157480314961 56 11.02098093575658 29.86959646780984 49.24530239244276 9.864120203990828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 1.5 7 1.0 8 3.5 9 6.0 10 21.0 11 36.0 12 59.5 13 117.5 14 152.0 15 204.5 16 257.0 17 335.5 18 414.0 19 490.5 20 567.0 21 649.0 22 922.0 23 1113.0 24 1381.5 25 1650.0 26 1939.5 27 2229.0 28 2443.0 29 2700.0 30 2743.0 31 2570.5 32 2398.0 33 2039.0 34 1680.0 35 1387.5 36 1095.0 37 906.5 38 622.5 39 527.0 40 495.5 41 464.0 42 433.5 43 403.0 44 407.0 45 411.0 46 476.5 47 574.5 48 607.0 49 641.5 50 676.0 51 650.0 52 624.0 53 656.5 54 715.5 55 742.0 56 760.5 57 779.0 58 738.0 59 697.0 60 692.0 61 687.0 62 695.0 63 685.0 64 667.0 65 586.0 66 505.0 67 423.0 68 341.0 69 290.0 70 239.0 71 215.5 72 159.0 73 126.0 74 93.5 75 61.0 76 47.5 77 34.0 78 23.5 79 9.0 80 5.0 81 5.0 82 5.0 83 3.5 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.013663068725235688 14 0.003415767181308922 15 0.21177756524115315 16 0.07514687798879628 17 0.0 18 0.0 19 0.09222571389534089 20 0.07514687798879628 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.006831534362617844 27 0.09222571389534089 28 0.10588878262057658 29 0.02049460308785353 30 0.013663068725235688 31 0.030741904631780295 32 0.11272031698319443 33 0.15712529034021042 34 0.03415767181308922 35 0.03757343899439814 36 0.030741904631780295 37 0.16395682470282824 38 0.25959830577947807 39 0.19811449651591745 40 0.7036480393496379 41 0.877852165596393 42 0.11272031698319443 43 0.218609099603771 44 0.4679601038393223 45 0.19128296215329962 46 0.19128296215329962 47 0.023910370269162453 48 0.13663068725235689 49 0.4679601038393223 50 0.12979915288973903 51 0.2937559775925673 52 0.13321492007104796 53 0.15712529034021042 54 0.22544063396638886 55 0.22544063396638886 56 0.2015302636972264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 29276.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.04085257548846 #Duplication Level Percentage of deduplicated Percentage of total 1 88.40968020743301 69.87976499521793 2 6.853932584269663 10.8348134991119 3 2.1780466724286947 5.16463997813909 4 0.9291270527225584 2.937559775925673 5 0.5488331892826275 2.169012160131165 6 0.3197925669835782 1.5166006285011615 7 0.17718236819360417 0.9803251810356606 8 0.108038029386344 0.6831534362617844 9 0.09507346585998272 0.6763219018991666 >10 0.3673292999135696 4.5532176526847925 >50 0.01296456352636128 0.6045907910916792 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 71 0.24251946987293346 No Hit TCTACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.18103566060937287 No Hit TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 53 0.18103566060937287 No Hit TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC 46 0.15712529034021042 No Hit TCTACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 39 0.13321492007104796 No Hit TCTACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.12296761852712118 No Hit TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC 33 0.11272031698319443 No Hit TCTACCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31 0.10588878262057658 No Hit TCTACCGGGAAGCAACGCAGAGTCGACTTTTCTTTTTTTTCTTTTTTCTTTTTTTT 30 0.10247301543926765 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.003415767181308922 0.0 0.0 0.0 0.0 8 0.003415767181308922 0.0 0.0 0.0 0.0 9 0.003415767181308922 0.0 0.0 0.0 0.0 10 0.003415767181308922 0.0 0.0 0.0 0.0 11 0.013663068725235688 0.0 0.0 0.0 0.0 12 0.01707883590654461 0.0 0.0 0.0 0.0 13 0.01707883590654461 0.0 0.0 0.0 0.0 14 0.027326137450471376 0.0 0.0 0.0 0.0 15 0.0307419046317803 0.0 0.0 0.0 0.0 16 0.04440497335701599 0.0 0.0 0.0 0.0 17 0.047820740538324906 0.0 0.0 0.0 0.0 18 0.05806804208225167 0.0 0.0 0.0 0.0 19 0.0614838092635606 0.0 0.0 0.0 0.0 20 0.07173111080748736 0.0 0.0 0.0 0.0 21 0.08197841235141413 0.0 0.0 0.0 0.0 22 0.08880994671403197 0.0 0.0 0.0 0.0 23 0.09905724825795874 0.0 0.0 0.0 0.0 24 0.11613608416450334 0.0 0.0 0.0 0.0 25 0.14687798879628364 0.0 0.0 0.0 0.0 26 0.1537095231589015 0.0 0.0 0.0 0.0 27 0.16054105752151934 0.0 0.0 0.0 0.0 28 0.1878671949719907 0.0 0.0 0.0 0.0 29 0.1878671949719907 0.0 0.0 0.0 0.0 30 0.19811449651591748 0.0 0.0 0.0 0.0 31 0.19811449651591748 0.0 0.0 0.0 0.0 32 0.20836179805984423 0.0 0.0 0.0 0.0 33 0.21519333242246208 0.0 0.0 0.0 0.0 34 0.218609099603771 0.0 0.0 0.0 0.0 35 0.22202486678507993 0.0 0.0 0.0 0.0 36 0.22885640114769779 0.0 0.0 0.0 0.0 37 0.2356879355103156 0.0 0.0 0.0 0.0 38 0.24935100423555132 0.0 0.0 0.0 0.0 39 0.2527667714168602 0.0 0.0 0.0 0.0 40 0.25959830577947807 0.0 0.0 0.0 0.0 41 0.263014072960787 0.0 0.0 0.0 0.0 42 0.2800929088673316 0.0 0.0 0.0 0.0 43 0.29034021041125835 0.0 0.0 0.0 0.0 44 0.2971717447738762 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACAA 15 0.008102634 50.379967 37 ATATGCT 15 0.008102634 50.379967 34 ACAACCT 15 0.008102634 50.379967 40 CATATGC 20 4.102713E-4 50.034306 33 CAGCTAG 15 0.008326731 50.0343 33 TAGTGAG 15 0.008326731 50.0343 47 CCTGCCT 15 0.008326731 50.0343 44 CCAAAAC 15 0.008326731 50.0343 43 GTGAGTC 15 0.008326731 50.0343 49 TGCCTGC 15 0.008326731 50.0343 46 GTGGGTT 15 0.008326731 50.0343 42 ATAGTGA 15 0.008326731 50.0343 46 AGTGAGT 15 0.008326731 50.0343 48 CAGATTT 15 0.008326731 50.0343 49 TGGGTTT 15 0.008326731 50.0343 43 GCCTGCA 15 0.008326731 50.0343 47 TGAGTCC 15 0.008326731 50.0343 50 ATCCTGC 30 1.0200947E-6 49.94863 20 TCCTTCG 15 0.008383468 49.94863 16 TCTAGGC 15 0.008383468 49.94863 26 >>END_MODULE