FastQCFastQC Report
Fri 17 Jun 2016
SRR1526269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215961
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5370.24865600733465762No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC4560.21114923527859197No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4290.19864697792657007No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4150.19216432596626243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTCC150.00808016150.5263439
TCGCCTA150.0083232850.14892642
TGTAGCG204.115384E-450.14892642
TCGACGT150.00847796649.91589428
ATGATAC204.2352913E-449.85796710
CGGTAGT402.733941E-949.85796712
CGTGCAG150.00852478749.84640524
GGAGTAC204.2840242E-449.7425358
TAATGAG1050.049.7425352
GTAATGG217750.048.9658431
AATGGGG203250.048.9593773
TAATGGG215350.048.9456372
ATGGGGG89750.048.8557624
ATGGGGA83650.048.6126944
TGGGGAC15050.048.4204675
TGGGGGG39100.048.1522985
ATGGGGT15800.047.8535774
ATGGGGC15700.047.36634
GAACAGT950.047.2229089
TGGGGAG23500.047.2024885
Produced by FastQC (version 0.11.5)