##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526269_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 215961 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.63863845786971 39.0 38.0 39.0 34.0 40.0 2 37.11245085918291 39.0 37.0 39.0 32.0 40.0 3 36.12751376405925 39.0 37.0 39.0 29.0 40.0 4 36.07466625918568 39.0 37.0 39.0 28.0 40.0 5 37.19648918091693 39.0 38.0 39.0 33.0 40.0 6 37.232546617213295 39.0 38.0 39.0 33.0 40.0 7 37.55916577530202 39.0 38.0 40.0 34.0 40.0 8 37.4197563448956 39.0 38.0 40.0 33.0 40.0 9 37.363102597228206 39.0 38.0 39.0 33.0 40.0 10 36.84805126851607 39.0 37.0 39.0 32.0 40.0 11 35.98744680752543 38.0 35.0 39.0 30.0 40.0 12 34.23221322368391 38.0 33.0 39.0 22.0 40.0 13 34.085450613768224 38.0 33.0 39.0 21.0 40.0 14 34.194701821162155 38.0 33.0 39.0 22.0 40.0 15 34.16583549807604 38.0 33.0 39.0 22.0 40.0 16 33.555720708831686 38.0 32.0 39.0 21.0 40.0 17 33.481059080111685 37.0 31.0 39.0 21.0 40.0 18 32.92957061691694 37.0 30.0 39.0 18.0 40.0 19 33.826251036066694 37.0 31.0 39.0 23.0 40.0 20 33.86268817054931 37.0 31.0 39.0 23.0 40.0 21 34.583475720153174 38.0 33.0 39.0 25.0 40.0 22 34.2795875181167 38.0 33.0 39.0 23.0 40.0 23 34.84208722871259 38.0 33.0 39.0 26.0 40.0 24 34.4501090474669 38.0 33.0 39.0 24.0 40.0 25 34.74396303036196 38.0 33.0 39.0 26.0 40.0 26 35.31580239024639 38.0 35.0 39.0 28.0 40.0 27 35.21036205611198 38.0 35.0 39.0 27.0 40.0 28 35.18784873194697 38.0 35.0 39.0 27.0 40.0 29 34.996568824926726 38.0 35.0 39.0 27.0 40.0 30 34.890846032385475 38.0 34.0 39.0 27.0 40.0 31 35.11586351239344 38.0 35.0 39.0 28.0 40.0 32 34.83673904084534 38.0 35.0 39.0 27.0 40.0 33 34.97502326809007 38.0 35.0 39.0 27.0 40.0 34 34.78533624126578 38.0 34.0 39.0 27.0 40.0 35 34.65731312598108 38.0 34.0 39.0 27.0 40.0 36 34.075272850190544 37.0 33.0 39.0 27.0 40.0 37 33.8494265168248 37.0 33.0 39.0 26.0 40.0 38 33.54632086348924 36.0 33.0 39.0 24.0 40.0 39 33.02974611156644 36.0 32.0 38.0 22.0 40.0 40 29.816059381091957 35.0 25.0 38.0 9.0 39.0 41 26.690564500071773 32.0 15.0 38.0 5.0 39.0 42 30.561300419983237 35.0 29.0 38.0 10.0 39.0 43 29.193780358490653 34.0 26.0 38.0 4.0 39.0 44 29.13855279425452 34.0 26.0 38.0 4.0 39.0 45 30.19463699464255 34.0 28.0 38.0 4.0 39.0 46 29.824852635429544 35.0 27.0 38.0 4.0 40.0 47 29.368140543894498 35.0 26.0 38.0 4.0 40.0 48 29.393371951417155 35.0 26.0 38.0 4.0 40.0 49 26.966605081473045 33.0 15.0 38.0 4.0 39.0 50 29.032223410708415 34.0 26.0 38.0 4.0 39.0 51 29.342339589092475 35.0 27.0 39.0 4.0 40.0 52 29.068141933034205 35.0 26.0 39.0 4.0 40.0 53 28.72404739744676 35.0 25.0 39.0 4.0 40.0 54 27.7651844546006 34.0 18.0 38.0 4.0 40.0 55 28.05983487759364 34.0 23.0 38.0 4.0 40.0 56 26.833006885502474 33.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.8775683626384776 1 2 1.2878894111646133 1 3 2.6107846265914887 1 4 2.9783672568238018 1 5 1.0921731521210418 1 6 1.0731919873487286 1 7 1.0937733579028404 1 8 1.19292804333233 1 9 1.3515851546270525 1 10 1.0844563822907674 1 11 -3.924469790427196 1 12 -13.475946786040588 1 13 -8.512145421474802 1 14 -6.365820641684273 1 15 -6.280288806521952 1 16 -22.91284193406604 1 17 -5.3536480197597704 1 18 -13.77111855913073 1 19 -6.239463939547647 1 20 -6.1929942094425385 1 21 -7.003374414692097 1 22 -3.584091897246079 1 23 -3.961963003546277 1 24 -4.685608457609668 1 25 -4.0916560186414515 1 26 2.1195069247008504 1 27 2.019253508386015 1 28 2.258668412334245 1 29 2.3697801320503658 1 30 2.247393998189736 1 31 1.130431155899437 1 32 1.7165522703334801 1 33 0.9361639444719856 1 34 0.9344502255080371 1 35 1.3536262879317178 1 36 1.41698033370659 1 37 1.5114835452501367 1 38 1.4854092595427986 1 39 1.7933612883967385 1 40 4.349830240515388 1 41 -11.807611232797834 1 42 -12.021925998394337 1 43 -18.930633267953056 1 44 -22.210909036200498 1 45 -13.623731322366739 1 46 -18.36864411667178 1 47 -23.005048845038523 1 48 -16.43229630107494 1 49 -20.32107569378252 1 50 -15.525554126263321 1 51 -12.996555690375489 1 52 -12.626913126131756 1 53 -12.677900975367177 1 54 -11.802255470505123 1 55 -12.063537693503616 1 56 -7.667718637586315 2 1 1.4581005755236305 2 2 1.837902143530286 2 3 3.233410040393373 2 4 3.28680881526536 2 5 1.6754173588946628 2 6 1.7884962908883253 2 7 1.463304806846054 2 8 1.5942140122653612 2 9 1.5409408969239706 2 10 1.5550064204878637 2 11 0.8304830998198085 2 12 -2.8801866638098765 2 13 -1.8623746040569245 2 14 2.5763234886951523 2 15 2.748422678071883 2 16 2.0258135281188316 2 17 3.0470013308895787 2 18 -2.180769692311273 2 19 2.172504484185481 2 20 1.9730613036717983 2 21 2.324725916349408 2 22 2.1673086629780087 2 23 2.0079249516357933 2 24 1.1944781224769088 2 25 1.8495713814094756 2 26 1.811078098624968 2 27 2.15163191429383 2 28 1.9407921709285958 2 29 2.38898053948607 2 30 2.015550351640208 2 31 1.2769679524362303 2 32 1.6248410403869542 2 33 1.1147608377747602 2 34 1.096736958383005 2 35 1.1417215260269558 2 36 1.174148655580801 2 37 1.493569106747465 2 38 1.4530053889036338 2 39 2.1149553805790617 2 40 4.698837115992852 2 41 -8.151359641252125 2 42 -7.610543263482192 2 43 -14.833077882162378 2 44 -14.850481228713866 2 45 -6.985457831152072 2 46 -10.736354837323681 2 47 -17.91770481651802 2 48 -9.005036306167884 2 49 -12.799303348480766 2 50 -7.9460532349977235 2 51 -2.7773934800368103 2 52 -2.645948012813701 2 53 -2.5481581691537833 2 54 -1.465306145320504 2 55 -2.024322007229106 2 56 1.2286781029280718 3 1 1.5678027625873767 3 2 1.6764951466184002 3 3 3.104544606153347 3 4 3.352960090700023 3 5 1.7547777379293024 3 6 1.6535075011764064 3 7 1.5823982541153967 3 8 1.8173516238394498 3 9 1.467418328287394 3 10 1.382061287445012 3 11 2.0431613010973635 3 12 3.7567797899318194 3 13 2.4525604608781393 3 14 2.8273070328383056 3 15 2.79111695447952 3 16 3.982770252176536 3 17 4.410521361347719 3 18 4.675505953866654 3 19 3.7463443251263016 3 20 3.077274679872147 3 21 3.0644674451788845 3 22 3.3156334652116044 3 23 2.81609154074701 3 24 3.366291650967966 3 25 2.9824450224264396 3 26 2.4540409989168595 3 27 2.2710003308935214 3 28 2.18976632381154 3 29 1.9188252875718632 3 30 2.1757199865655394 3 31 1.6218535228918327 3 32 2.041557060523175 3 33 1.6964373046303791 3 34 1.5963139286440153 3 35 1.5173613585071806 3 36 2.332954019597885 3 37 2.336699103786252 3 38 2.7900014547270615 3 39 3.0543563704686534 3 40 6.299169194337956 3 41 6.268814647000337 3 42 3.252839282310781 3 43 -2.069318839901708 3 44 1.7992078444279755 3 45 3.4912332302295432 3 46 1.228660603261794 3 47 -3.925403957767653 3 48 1.6090653901813674 3 49 3.758249847508914 3 50 2.1065082100718655 3 51 3.3905810535729053 3 52 3.1045807743600378 3 53 3.3285498134173928 3 54 4.2032500541803195 3 55 3.337241510686205 3 56 4.700832170359014 4 1 1.6200607084139946 4 2 1.788521291823855 4 3 3.2170252623806945 4 4 3.4833506455281196 4 5 1.6639404636968322 4 6 1.6121145331112174 4 7 1.635835764260726 4 8 1.6790071261282407 4 9 1.5409408969239706 4 10 1.36012571979321 4 11 2.1362819521302896 4 12 4.368454230179857 4 13 4.269836208386444 4 14 3.803445204188961 4 15 4.182808060131684 4 16 4.628803561341421 4 17 4.971268923761826 4 18 4.816361083889269 4 19 3.8873942455868686 4 20 3.345003315148695 4 21 3.0048074627100263 4 22 3.5086707892238564 4 23 2.9506159815361315 4 24 3.394568729544261 4 25 3.037430034384407 4 26 2.517813223964744 4 27 2.027122702575319 4 28 2.376159129551368 4 29 2.1884368970819637 4 30 2.285332894678561 4 31 1.8588918424996592 4 32 2.337541130994026 4 33 1.7925772074515933 4 34 1.2880688822730662 4 35 1.660824516060181 4 36 2.6202374505067993 4 37 2.9250852650078087 4 38 3.05169158642704 4 39 3.6025724147077227 4 40 6.8985501936129054 4 41 9.32025014431115 4 42 5.590151390700832 4 43 6.852364582349853 4 44 6.12354474531211 4 45 5.5541072549246415 4 46 5.179984637155332 4 47 4.618689022201401 4 48 4.817902383049873 4 49 7.573393721890721 4 50 4.68170272996754 4 51 3.940138987495658 4 52 4.353674457739 4 53 4.146345002093575 4 54 4.915847584670434 4 55 3.9690334744984703 4 56 4.416914285350302 5 1 1.577542391985311 5 2 1.6713071491207216 5 3 3.1394692130088373 5 4 3.3988970581483073 5 5 1.7753571541986943 5 6 1.6936308913871656 5 7 1.4612191441643603 5 8 1.6869937490848272 5 9 1.486541653783 5 10 1.5895596156370857 5 11 2.5084739831087077 5 12 4.362502765965644 5 13 3.9492492025999937 5 14 4.223050933435843 5 15 4.349889092452209 5 16 4.746721758960838 5 17 4.479464563021686 5 18 5.320090374773962 5 19 3.8546666928377533 5 20 3.9067071280461008 5 21 3.0750699431835002 5 22 3.2507996752769657 5 23 3.096146332903537 5 24 3.282161961816378 5 25 3.1368337878771797 5 26 2.126765722259613 5 27 2.588309475143575 5 28 2.436190812022602 5 29 2.1902190360888056 5 30 2.326916138171555 5 31 2.012991862301199 5 32 2.2989831234694336 5 33 1.5235292217087064 5 34 1.5246203332862436 5 35 1.7209552062540183 5 36 2.533484683790995 5 37 2.7884216052423483 5 38 3.02832576389288 5 39 3.2287852593096034 5 40 6.6717235011308915 5 41 8.8783126731884 5 42 5.728366171453736 5 43 6.653161044473901 5 44 6.6751057670718055 5 45 5.461777053168905 5 46 5.3780770883665205 5 47 5.482355388177943 5 48 5.571703179511996 5 49 8.011997933019192 5 50 6.195728824002877 5 51 5.190848542840975 5 52 5.1304560467029425 5 53 5.656795817257155 5 54 6.062775045012341 5 55 5.2968700457510245 5 56 5.661485361894272 6 1 1.2568810633285068 6 2 1.1459477564539498 6 3 2.951892777168794 6 4 3.2815031453255443 6 5 1.3466242008332117 6 6 1.3171627463840494 6 7 1.4207802582239424 6 8 1.3731972241753994 6 9 1.3318816634744906 6 10 1.4853991984416126 6 11 2.078344994023169 6 12 3.8777322465480566 6 13 4.107612725686501 6 14 4.148068816538043 6 15 3.8433704132635214 6 16 4.55368809257876 6 17 4.606630725884827 6 18 4.744000659288346 6 19 4.1291088554240005 6 20 3.878509513998054 6 21 2.941846600543265 6 22 3.175148384232365 6 23 2.9286566589528675 6 24 3.238665642274185 6 25 2.719384495125027 6 26 1.8744293814884472 6 27 2.10831547463593 6 28 1.4464937863863057 6 29 1.9147961872529393 6 30 1.8783156351371986 6 31 1.4702685542734173 6 32 1.7557406286036183 6 33 1.3345106002074516 6 34 1.4792203286712535 6 35 1.5171570660966438 6 36 1.8529576514629653 6 37 1.8965624548627602 6 38 2.444690533027803 6 39 2.7014298816877087 6 40 5.835913454288701 6 41 1.6286530290044468 6 42 3.4529663975884475 6 43 4.814847363323846 6 44 4.489398403848696 6 45 3.6285744780021894 6 46 4.537005948232231 6 47 4.769973358965032 6 48 4.437868350151408 6 49 6.7426668764162905 6 50 4.729429818534108 6 51 3.988919475300534 6 52 3.931261363176162 6 53 4.082978986373618 6 54 4.817557332664926 6 55 3.871148376046154 6 56 4.437658147273972 7 1 1.3455013691744213 7 2 1.5565168621450027 7 3 3.024265018748352 7 4 3.3498950346015803 7 5 1.5999754397027388 7 6 1.48699917689121 7 7 1.4398109396021823 7 8 1.584022814574162 7 9 1.494313912796983 7 10 1.5033289313103708 7 11 2.4098030853897967 7 12 3.845417593044381 7 13 4.341449349767025 7 14 3.6215976589693213 7 15 4.141279820929029 7 16 4.37204368684899 7 17 4.5574630913513445 7 18 4.807190264398685 7 19 3.7918677597987553 7 20 3.7330270323875254 7 21 2.9358821212556165 7 22 3.093399932819281 7 23 2.709809872270718 7 24 2.9095304312792223 7 25 2.6441691447572424 7 26 1.7721621534590213 7 27 2.036778018189935 7 28 1.658505013641438 7 29 2.303031766037293 7 30 1.8122224295622829 7 31 1.508208933677217 7 32 1.8284395252354386 7 33 1.7136966242105487 7 34 1.2300793104753538 7 35 1.4487651768206078 7 36 1.6570130134451588 7 37 1.3332155713939287 7 38 1.69660207000031 7 39 2.6806570400307237 7 40 4.955916841822578 7 41 -0.200421690314176 7 42 3.7511540669651353 7 43 5.529701699367205 7 44 4.965806650074015 7 45 3.246775213580971 7 46 4.180988236269396 7 47 4.56745932489612 7 48 4.201854025722451 7 49 6.94853214935473 7 50 4.76133312863864 7 51 4.675258035114709 7 52 4.938242102626415 7 53 4.941380070384454 7 54 5.548357274592913 7 55 4.6041502149931155 7 56 4.787796068296039 8 1 1.2958927833158356 8 2 1.8625774682056147 8 3 3.1405528975362316 8 4 3.4110945295510717 8 5 1.7881446316219325 8 6 1.4336910960831304 8 7 1.3234346769759213 8 8 1.5746036226549691 8 9 1.54509674107981 8 10 1.5727986282800686 8 11 2.4000934894301977 8 12 4.425657492034276 8 13 4.158015006332683 8 14 3.8480118003834605 8 15 4.3321889118381165 8 16 4.854644696950004 8 17 4.497261071149325 8 18 4.884425112883534 8 19 3.8000369517179493 8 20 3.8486457192562114 8 21 2.911349292972787 8 22 3.0388671045364504 8 23 3.013509367220209 8 24 3.7022703302691227 8 25 3.2137272255653215 8 26 2.2760131635600303 8 27 2.2800734727353884 8 28 2.134818144954572 8 29 2.424824695330223 8 30 2.327108793198647 8 31 1.5970978225661057 8 32 2.173022858568771 8 33 1.7982673312812523 8 34 2.3047889064349505 8 35 1.8602929545983855 8 36 1.9590837205158635 8 37 1.7581145612929134 8 38 1.9515768174750576 8 39 3.0279423935660787 8 40 4.815070882226621 8 41 -2.344866134758618 8 42 3.40374245080352 8 43 5.176532507448012 8 44 5.127700589467949 8 45 3.5831135974193593 8 46 4.549359448390607 8 47 4.893204274391067 8 48 4.345742914611339 8 49 7.064592755415337 8 50 4.681219492275005 8 51 5.228970156326831 8 52 4.792623415757728 8 53 5.22249118149557 8 54 5.8333951533807955 8 55 5.193859810952713 8 56 5.411924856174824 9 1 1.0901352075582622 9 2 1.461819892448034 9 3 3.0223710793544143 9 4 3.171549075005622 9 5 1.3534476619249602 9 6 1.3209638233558607 9 7 1.2084346769759193 9 8 1.1932399862913314 9 9 1.1919149228979933 9 10 1.346131961613402 9 11 2.0875177318544402 9 12 4.165809007185793 9 13 4.051045309362991 9 14 4.221345133716795 9 15 3.8117343663835754 9 16 4.584796212101516 9 17 4.135745919634168 9 18 4.556243294701716 9 19 3.787764224445219 9 20 3.1272820828925774 9 21 2.6558947475182393 9 22 3.343715589384935 9 23 2.8060851247959704 9 24 3.3530279060266963 9 25 2.7050908619289586 9 26 2.137376799923672 9 27 2.172194684856599 9 28 1.7746666298030505 9 29 2.033461058966587 9 30 2.2904421265319783 9 31 1.2683099437782275 9 32 1.2536289191748367 9 33 1.3772067252206455 9 34 1.0826676943137414 9 35 1.009686893992324 9 36 2.0269625083946536 9 37 1.9632660764444338 9 38 2.3361222720205177 9 39 2.521881787505471 9 40 5.374161791317526 9 41 3.0369520470595646 9 42 3.9560151780762496 9 43 5.208047658963164 9 44 5.198761195528554 9 45 4.230992385298148 9 46 5.208450357481514 9 47 5.385325486512279 9 48 5.124833823702243 9 49 7.736410937233519 9 50 5.247128583184093 9 51 4.805939853296522 9 52 4.809138567272878 9 53 5.154763908768295 9 54 5.644001213986858 9 55 4.464314356407257 9 56 4.2357127349627035 10 1 1.3346622268001056 10 2 1.8993558989003887 10 3 3.300801774134918 10 4 3.5943368385728505 10 5 1.8669140751062017 10 6 1.849826595564636 10 7 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1.3596777816852352 116 3 3.027856972771346 116 4 3.185263808547937 116 5 1.4410913400858831 116 6 1.376606456584696 116 7 1.267995805502565 116 8 1.246139672811708 116 9 1.0261625718008176 116 10 1.5155415749885321 116 11 -3.013448830319014 116 12 -13.450439686649108 116 13 -9.948301608087377 116 14 -4.1006245632529 116 15 -5.324237420450281 116 16 -3.7081766196644175 116 17 -1.8639928473459761 116 18 -7.583255137900167 116 19 -2.9877059949905167 116 20 -3.675617603627799 116 21 1.8102834622517818 116 22 2.7953874807120016 116 23 1.6852648531136296 116 24 2.4132421171029748 116 25 2.376329106443066 116 26 2.365433226255959 116 27 2.4710452595692445 116 28 1.9108996496567627 116 29 2.243884256458749 116 30 2.3520931192174572 116 31 1.8195116156695477 116 32 2.455953893051536 116 33 1.703878616547712 116 34 1.967986398597958 116 35 2.5013274373570056 116 36 2.886419143713354 116 37 3.0061082917004427 116 38 2.6288338707665986 116 39 3.6714376913717217 116 40 7.159820098527561 116 41 4.6396166238620715 116 42 -0.6388124081306472 116 43 -7.6476210975676615 116 44 -6.7446818556731145 116 45 -0.5192688477217082 116 46 -4.616784752236352 116 47 -2.6704477632264876 116 48 -4.288567430216251 116 49 -7.4379621035188315 116 50 -4.078106526533773 116 51 -1.724885643046214 116 52 -1.6444925717030863 116 53 -3.0854398529871894 116 54 -6.966369736901338 116 55 -2.5329426968842768 116 56 -1.0415861156120094 117 1 0.2878164397698768 117 2 0.726866770063971 117 3 2.181838683573403 117 4 2.420356207100042 117 5 0.7371953598991823 117 6 0.5781210307893119 117 7 0.503826439909119 117 8 0.5937296899578328 117 9 0.4935849379657995 117 10 0.6049633696160797 117 11 -3.5390727586863235 117 12 -9.931650393450404 117 13 -10.254621859563851 117 14 -11.78501682844627 117 15 -10.369769902827876 117 16 -16.37316042610374 117 17 -14.675194981085738 117 18 -14.952420693244044 117 19 -6.604061491275523 117 20 -7.57293137768 117 21 -1.0134924950734217 117 22 0.15645625571676192 117 23 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118 6 0.6441961465881789 118 7 0.48242457596582256 118 8 0.570765238816584 118 9 0.44065229663537053 118 10 0.8006774161588552 118 11 -3.3253610560243487 118 12 -8.615756649379865 118 13 -10.385823377505698 118 14 -10.839260926889263 118 15 -7.267811088161881 118 16 -14.179092676787374 118 17 -13.038496504608254 118 18 -13.264463776005357 118 19 -6.082185270504272 118 20 -6.278020947410454 118 21 -0.5183476767241828 118 22 0.5608873065566513 118 23 -0.1749754812646387 118 24 0.11439154239032945 118 25 -0.00829297645488225 118 26 1.148740436287305 118 27 1.4600734727353881 118 28 1.1370403671767875 118 29 1.0296731801787118 118 30 1.39902798511784 118 31 -0.03219510672682446 118 32 -4.069603404057489 118 33 0.3885703615842857 118 34 -0.00571614407009946 118 35 0.5677677020731338 118 36 1.0219120033441484 118 37 1.3041751673535202 118 38 1.3436980295962755 118 39 1.8956191612428412 118 40 5.002949670105409 118 41 1.0319015420090594 118 42 -3.74979290273183 118 43 -10.110134159218656 118 44 -10.254521632754269 118 45 -4.965977311671551 118 46 -11.92538802635687 118 47 -12.569219968033174 118 48 -12.68072173185331 118 49 -12.670154719332137 118 50 -10.042012830957319 118 51 -8.09810055074388 118 52 -8.10374022060591 118 53 -7.7025593235549366 118 54 -11.939332119346478 118 55 -7.332402815309916 118 56 -4.828681204431234 119 1 0.5091209836868913 119 2 0.7163808077232332 119 3 2.351220801061281 119 4 2.5651019507013544 119 5 0.7148422197480926 119 6 0.7912792222426859 119 7 0.4424328216883495 119 8 0.5807631773859541 119 9 0.6991041622300926 119 10 0.78691736668452 119 11 -5.854581835245124 119 12 -12.143173676796891 119 13 -12.139276273815739 119 14 -10.643991910191676 119 15 -10.792393648458727 119 16 -13.948676267799534 119 17 -15.966913325882217 119 18 -17.39922980362853 119 19 -7.6085715826048705 119 20 -8.841873761263269 119 21 -0.9544948628713712 119 22 0.34588009154943933 119 23 -0.41044239530297943 119 24 0.003847324703258437 119 25 -0.2844731454921927 119 26 1.375549341667643 119 27 0.8881810794144229 119 28 1.143482337904473 119 29 1.287867366944326 119 30 1.6291867152765676 119 31 -0.02637156518900241 119 32 -3.613099590410819 119 33 -0.051751221594443564 119 34 -0.2274745443999322 119 35 -0.37358459642202035 119 36 0.29845292274220725 119 37 0.5863334852261275 119 38 0.1380703239685701 119 39 0.8413004640670039 119 40 4.458113553840718 119 41 1.0949297836087268 119 42 -3.628168495393137 119 43 -9.235675284759779 119 44 -8.447883826116463 119 45 -5.375532042654854 119 46 -17.164462444002716 119 47 -14.4469255957388 119 48 -14.534257085388663 119 49 -13.330379415271118 119 50 -12.133437279524626 119 51 -10.044554890055363 119 52 -9.527673430253405 119 53 -10.100570042994228 119 54 -11.11001857574722 119 55 -9.866158741922984 119 56 -6.89608998612573 120 1 -0.2908933882590645 120 2 -0.20874135381743741 120 3 1.6612955351802796 120 4 1.5910433131618333 120 5 0.016006347370975504 120 6 -0.044593154877176744 120 7 -0.07725674427887697 120 8 -0.25410316351660356 120 9 -0.12002489784464387 120 10 -0.11844336915910958 120 11 -5.09552750928939 120 12 -14.20353584983512 120 13 -14.322087426445805 120 14 -14.165765408323324 120 15 -13.5770300382259 120 16 -17.879619067454605 120 17 -15.933929709900614 120 18 -16.72640715344169 120 19 -8.03153795225132 120 20 -14.407769133496668 120 21 -6.606909349792897 120 22 -4.22756055231374 120 23 -4.254147482971849 120 24 -10.397345546811547 120 25 -3.977847678403947 120 26 0.9918902442258428 120 27 1.0336970322744392 120 28 0.6184032922016911 120 29 0.8181921729230552 120 30 1.287134401393267 120 31 -0.7437472478564331 120 32 -10.25063911326221 120 33 0.08233903422619449 120 34 -0.2663506582257398 120 35 -1.165622538359358 120 36 -0.10726711175045978 120 37 -0.1994526602179647 120 38 -0.44140653719843215 120 39 0.45124585067235046 120 40 3.0306918809462076 120 41 -2.158950641800871 120 42 -6.12673130079699 120 43 -10.631047518160184 120 44 -9.924744993118477 120 45 -8.63947283023749 120 46 -16.68623594213436 120 47 -16.99068817119152 120 48 -14.492003564261903 120 49 -18.559683813086583 120 50 -14.420445040375444 120 51 -12.690645720753839 120 52 -12.410398351207702 120 53 -12.056663748081895 120 54 -14.036003054045581 120 55 -13.1017867959615 120 56 -10.41276144344105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 999.0 5 92.0 6 189.0 7 279.0 8 406.0 9 464.0 10 349.0 11 314.0 12 295.0 13 309.0 14 422.0 15 543.0 16 764.0 17 940.0 18 1129.0 19 1308.0 20 1773.0 21 2137.0 22 2683.0 23 3218.0 24 3833.0 25 4195.0 26 4643.0 27 5231.0 28 5756.0 29 6947.0 30 8091.0 31 9542.0 32 10810.0 33 12761.0 34 14626.0 35 17514.0 36 22634.0 37 27959.0 38 26560.0 39 16234.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.23921448780104 0.5565819754492709 0.07455049754353796 0.12965303920615295 8 98.4418482966832 1.2812498552979474 0.11483554901116405 0.1620662990076912 9 93.55763309115997 3.5524932742485915 1.8484819018248666 1.0413917327665643 10 43.89866688892902 40.065567394112826 8.38901468320669 7.646751033751464 11 34.98594653664319 29.1659142159927 19.51278240052602 16.335356846838085 12 36.50196100221799 15.93991507725932 22.100749672394553 25.457374248128133 13 25.396082935436514 16.36293747423898 26.384843025652188 31.856136564672322 14 24.162792420678286 20.043434773750207 28.874863398099613 26.91890940747189 15 28.232460577485313 18.364998190137644 27.716417771920216 25.686123460456827 16 32.45468529913262 21.464248647045743 24.14838016161317 21.932685892208468 17 29.995230620343488 22.907376794884264 24.10990873352133 22.98748385125092 18 28.22208330439212 23.799865712764234 23.90405408534185 24.073996897501797 19 27.068470798741 27.34289171035614 22.778789859405638 22.809847631497217 20 27.840082303763914 26.92806762255197 22.91483228754414 22.317017786139974 21 26.894207750473463 24.478030755553085 25.745389213793228 22.88237228018022 22 26.68583679460643 23.59731618208843 26.555720708831686 23.161126314473446 23 26.08248711572923 23.68529503012118 27.34382596857766 22.88839188557193 24 28.00994633240569 24.114762524368054 25.651628318338204 22.22366282488806 25 26.957592820693293 24.12134065588042 25.185456161960417 23.735610361465866 26 23.633037719684165 22.738324032695022 30.11415472248593 23.51448352513488 27 23.383444356367715 20.40691448676763 31.080423324572017 25.12921783229264 28 22.489196357364097 23.17775469703433 30.53443255374001 23.798616391861565 29 25.084186781169954 22.405495416626138 30.539768121580995 21.970549680622913 30 23.942311700220916 20.680903486955756 34.40086328669547 20.975921526127852 31 24.317714412761937 20.953995516276656 34.91282678376225 19.815463287199155 32 25.652967782597415 19.742891039187334 35.30521262002744 19.298928558187818 33 24.901084934760124 20.016327363641 37.15681226778732 17.92577543381156 34 20.508853042277796 21.01659424251128 42.007245503988734 16.46730721122219 35 19.49041045121838 24.096173445751877 40.98767719818401 15.425738904845735 36 19.41469650930112 25.407618765285704 38.72424590528422 16.453438820128955 37 21.649934121402193 23.976562065062073 37.34063874403845 17.03286506949728 38 21.395402832598094 24.754121198049685 37.222660784768976 16.627815184583238 39 22.476815231662112 22.36500874503018 37.4676990596193 17.690476963688408 40 17.00436379911605 16.34653040672846 33.7075508643679 32.94155492978759 41 13.933189806144894 16.22433206151432 32.23991145524427 37.60256667709652 42 18.010507133815256 21.728344685922014 39.12075784905153 21.140390331211194 43 16.635961594683767 17.823018130687593 38.647553726141695 26.893466548486945 44 17.27405146056226 17.95767929524439 38.1035261049036 26.664743139289754 45 20.477852124766827 21.102892726884633 39.05134891835958 19.367906229988957 46 19.32181375391395 23.030035950365303 38.510959062971125 19.137191232749622 47 18.21238987243267 21.212584419554766 39.36568374049267 21.209341967519897 48 18.94804694927123 21.895593056850167 40.81859792150698 18.337762072371625 49 17.8779868008303 17.448874161089442 34.986177174186224 29.686961863894034 50 18.544311427220208 21.8121458841421 39.20984059570286 20.433702092934837 51 18.525588329548146 22.289875669374354 40.127628150645805 19.0569078504317 52 17.928937022476077 22.467268748609154 39.59461464283065 20.00917958608412 53 18.08901422038348 22.319415971726205 40.675120358431585 18.916449449458735 54 16.230074943734195 21.17404116104782 38.64080373094503 23.955080164272953 55 18.362620327308846 23.176176817325274 38.037353681064175 20.423849174301704 56 18.53422280778259 23.99725363939172 36.65509978752819 20.813423765297507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 2.0 4 4.5 5 5.0 6 51.5 7 98.0 8 253.5 9 409.0 10 593.0 11 777.0 12 835.0 13 968.5 14 1044.0 15 1275.0 16 1506.0 17 1814.5 18 2123.0 19 2266.0 20 2409.0 21 2789.0 22 3931.0 23 4693.0 24 5563.0 25 6433.0 26 7176.5 27 7920.0 28 8317.5 29 8762.5 30 8810.0 31 8175.5 32 7541.0 33 6660.0 34 5779.0 35 5177.5 36 4576.0 37 4313.0 38 4030.0 39 4010.0 40 4127.0 41 4244.0 42 4408.0 43 4572.0 44 5026.5 45 5481.0 46 5736.5 47 6326.5 48 6661.0 49 7190.0 50 7719.0 51 8358.5 52 8998.0 53 9416.0 54 10649.0 55 11464.0 56 11902.5 57 12341.0 58 13294.0 59 14247.0 60 13907.5 61 13568.0 62 12024.0 63 9240.0 64 8000.0 65 6952.5 66 5905.0 67 5017.5 68 4130.0 69 3462.5 70 2795.0 71 2338.5 72 1585.5 73 1289.0 74 1002.5 75 716.0 76 547.0 77 378.0 78 282.0 79 128.5 80 71.0 81 49.0 82 27.0 83 18.0 84 9.0 85 6.5 86 4.0 87 2.5 88 1.5 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01574358333217572 14 0.0023152328429670173 15 0.21994712008186665 16 0.06343737989729627 17 0.0 18 0.0027782794115604205 19 0.10835289705085639 20 0.0810331495038456 21 0.0 22 0.0 23 0.0 24 9.260931371868068E-4 25 0.003241325980153824 26 0.01203921078342849 27 0.11113117646241683 28 0.1356726445978672 29 0.033339352938725045 30 0.019447955880922945 31 0.03195021323294484 32 0.08195924264103241 33 0.17225332351674608 34 0.04769379656512056 35 0.046767703427933745 36 0.03380239950731845 37 0.19123823282907562 38 0.2829214534105695 39 0.1898490931232954 40 0.6802154092637096 41 0.848301313663115 42 0.13798787744083424 43 0.21763188723889962 44 0.4991642009436889 45 0.21068618870999858 46 0.17873597547705372 47 0.034728492644505254 48 0.15002708822426272 49 0.5088881788841504 50 0.13104217891193318 51 0.30005417644852544 52 0.1227073406772519 53 0.16438153185065824 54 0.21577970096452603 55 0.23476461027685555 56 0.1866077671431416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 215961.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.32349049335718 #Duplication Level Percentage of deduplicated Percentage of total 1 85.9664188447077 62.173914760631796 2 7.930805120464242 11.471670174691283 3 2.684160011853345 5.823834632997746 4 1.211142053264172 3.5037608310142567 5 0.6485522800501053 2.345278233032446 6 0.3805906776486702 1.651538775407038 7 0.2629412548665209 1.3311780542655165 8 0.1841919093536922 1.0657121444076052 9 0.13596152597669617 0.8849890918294129 >10 0.558282040510774 6.77287710049871 >50 0.025736017336775618 1.2456516819369656 >100 0.010558366086882305 1.4733052651875145 >500 6.59897880430144E-4 0.25628925409967457 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 537 0.24865600733465762 No Hit GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 456 0.21114923527859197 No Hit GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 429 0.19864697792657007 No Hit GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 415 0.19216432596626243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.630465685934034E-4 0.0 0.0 0.0 0.0 8 9.260931371868068E-4 0.0 0.0 0.0 0.0 9 0.0018521862743736137 0.0 0.0 0.0 0.0 10 0.007408745097494455 0.0 0.0 0.0 0.0 11 0.008797884803274666 0.0 0.0 0.0 0.0 12 0.010650071077648279 0.0 0.0 0.0 0.0 13 0.012965303920615297 0.0 0.0 0.0 0.0 14 0.013891397057802103 0.0 0.0 0.0 0.0 15 0.01481749019498891 0.0 0.0 0.0 0.0 16 0.016669676469362522 0.0 0.0 0.0 0.0 17 0.01852186274373614 0.0 0.0 0.0 0.0 18 0.025930607841230593 0.0 0.0 0.0 0.0 19 0.031950213232944835 0.0 0.0 0.0 0.0 20 0.036580678918878874 0.0 0.0 0.0 0.0 21 0.0398220048990327 0.0 0.0 0.0 0.0 22 0.043989424016373325 0.0 0.0 0.0 0.0 23 0.05047207597668098 0.0 0.0 0.0 0.0 24 0.054639495094021606 0.0 0.0 0.0 0.0 25 0.05834386764276883 0.0 0.0 0.0 0.0 26 0.06112214705432925 0.0 0.0 0.0 0.0 27 0.06436347303448307 0.0 0.0 0.0 0.0 28 0.07177221813197754 0.0 0.0 0.0 0.0 29 0.07686573038650497 0.0 0.0 0.0 0.0 30 0.07918096322947199 0.0 0.0 0.0 0.0 31 0.08242228920962581 0.0 0.0 0.0 0.0 32 0.08288533577821922 0.0 0.0 0.0 0.0 33 0.08334838234681262 0.0 0.0 0.0 0.0 34 0.08473752205259283 0.0 0.0 0.0 0.0 35 0.08658970832696644 0.0 0.0 0.0 0.0 36 0.09214626715008728 0.0 0.0 0.0 0.0 37 0.09353540685586749 0.0 0.0 0.0 0.0 38 0.09677673283602131 0.0 0.0 0.0 0.0 39 0.09909196567898834 0.0 0.0 0.0 0.0 40 0.10001805881617515 0.0 0.0 0.0 0.0 41 0.10048110538476855 0.0 0.0 0.0 0.0 42 0.10372243136492237 0.0 0.0 0.0 0.0 43 0.10603766420788939 0.0 0.0 0.0 0.0 44 0.10881594361944981 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCTCC 15 0.008080161 50.52634 39 TCGCCTA 15 0.00832328 50.148926 42 TGTAGCG 20 4.115384E-4 50.148926 42 TCGACGT 15 0.008477966 49.915894 28 ATGATAC 20 4.2352913E-4 49.857967 10 CGGTAGT 40 2.733941E-9 49.857967 12 CGTGCAG 15 0.008524787 49.846405 24 GGAGTAC 20 4.2840242E-4 49.742535 8 TAATGAG 105 0.0 49.742535 2 GTAATGG 21775 0.0 48.965843 1 AATGGGG 20325 0.0 48.959377 3 TAATGGG 21535 0.0 48.945637 2 ATGGGGG 8975 0.0 48.855762 4 ATGGGGA 8365 0.0 48.612694 4 TGGGGAC 1505 0.0 48.420467 5 TGGGGGG 3910 0.0 48.152298 5 ATGGGGT 1580 0.0 47.853577 4 ATGGGGC 1570 0.0 47.3663 4 GAACAGT 95 0.0 47.222908 9 TGGGGAG 2350 0.0 47.202488 5 >>END_MODULE