FastQCFastQC Report
Fri 17 Jun 2016
SRR1526268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65967
Sequences flagged as poor quality0
Sequence length56
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1980.3001500750375188No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1580.23951369624206043No Hit
CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1570.23799778677217395No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1200.18190913638637501No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1170.17736140797671565No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGTTCTTCC860.1303682144102354No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC790.11975684812103021No Hit
CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACCCTCCTCGTTCGCGCA670.10156593448239272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCAAA150.00806679350.507741
CGAAGCC150.00806679350.507741
TTACTTT150.00814082450.39123537
CGCTCTT251.9996627E-550.27530749
ACCGCTC204.074385E-450.19831547
TTACATT150.0082653350.19831546
CTAGCAA204.074385E-450.19831546
CCTCCTG150.00829040150.1599150
CTTCCTA150.00831552850.12155542
TGATTGC150.00834071250.0832744
TGCCTAG150.00834071250.0832743
CTGATTG150.00834071250.0832743
GTTGGGA150.00841660249.96874632
GATTTAG204.1834376E-449.93069519
CAATTGT204.1834376E-449.93069527
TGTTGTT204.1834376E-449.93069518
GAGGCCA204.1834376E-449.93069517
TCCGGAA150.008442013549.9306917
GTAGGAT150.008442013549.9306933
GCCGATG150.008442013549.9306928