##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526268_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65967 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.06730638046296 39.0 38.0 40.0 35.0 40.0 2 37.87943971985993 39.0 38.0 40.0 35.0 40.0 3 37.64207861506511 39.0 38.0 40.0 34.0 40.0 4 36.68637348977519 39.0 38.0 40.0 31.0 40.0 5 36.92083920748253 39.0 36.0 39.0 32.0 40.0 6 35.40961389785802 39.0 36.0 39.0 23.0 40.0 7 37.31415707853927 39.0 38.0 40.0 33.0 40.0 8 37.26049388330529 39.0 38.0 40.0 33.0 40.0 9 37.31365682841421 39.0 38.0 40.0 33.0 40.0 10 36.21245471220459 39.0 35.0 40.0 29.0 40.0 11 35.16815983749451 38.0 33.0 39.0 27.0 40.0 12 33.61538344930041 38.0 31.0 39.0 20.0 40.0 13 33.560568162869316 38.0 31.0 39.0 20.0 40.0 14 33.760804644746614 38.0 32.0 39.0 20.0 40.0 15 33.789106674549394 38.0 32.0 39.0 20.0 40.0 16 33.8785604923674 38.0 32.0 39.0 22.0 40.0 17 33.76949080600907 38.0 32.0 39.0 22.0 40.0 18 33.25946306486576 37.0 31.0 39.0 19.0 40.0 19 34.188442706201585 38.0 32.0 39.0 24.0 40.0 20 34.134248942653144 38.0 32.0 39.0 24.0 40.0 21 34.93149605105583 38.0 33.0 39.0 26.0 40.0 22 34.53328179241136 38.0 33.0 39.0 24.0 40.0 23 35.047508602786245 38.0 34.0 39.0 26.0 40.0 24 34.63831915957979 38.0 33.0 39.0 25.0 40.0 25 34.937574848030074 38.0 33.0 39.0 26.0 40.0 26 35.42741067503449 38.0 35.0 39.0 28.0 40.0 27 35.318613852380736 38.0 35.0 39.0 28.0 40.0 28 35.3084572589325 38.0 35.0 39.0 28.0 40.0 29 35.06153076538269 38.0 35.0 39.0 27.0 40.0 30 34.92476541300954 38.0 34.0 39.0 27.0 40.0 31 35.18174238634469 38.0 35.0 39.0 28.0 40.0 32 34.830384889414404 38.0 34.0 39.0 27.0 40.0 33 34.99940879530674 38.0 35.0 39.0 27.0 40.0 34 34.75507450695044 38.0 34.0 39.0 27.0 40.0 35 34.63583306804917 38.0 34.0 39.0 27.0 40.0 36 34.059226582988465 37.0 33.0 39.0 27.0 40.0 37 33.85820182818682 36.0 33.0 38.0 26.0 40.0 38 33.53222065578244 36.0 32.0 38.0 25.0 40.0 39 33.15019631027635 36.0 32.0 38.0 23.0 40.0 40 29.714630042293873 35.0 24.0 38.0 9.0 39.0 41 26.598587172374067 32.0 15.0 38.0 5.0 39.0 42 30.520790698379493 34.0 28.0 38.0 10.0 39.0 43 29.054936559188686 33.0 26.0 38.0 5.0 39.0 44 28.991025815938272 33.0 26.0 38.0 4.0 39.0 45 30.120090348204407 34.0 28.0 38.0 4.0 39.0 46 29.66916791729198 35.0 27.0 38.0 4.0 40.0 47 29.25221701759971 34.0 26.0 38.0 4.0 40.0 48 29.295011141934605 34.0 26.0 38.0 4.0 40.0 49 26.6675610532539 33.0 15.0 38.0 4.0 39.0 50 28.94876225991784 34.0 26.0 38.0 4.0 39.0 51 29.226810374884412 35.0 27.0 39.0 4.0 40.0 52 28.91880788879288 35.0 26.0 38.0 4.0 40.0 53 28.524792699379994 34.0 24.0 38.0 4.0 40.0 54 27.5513665923871 33.0 18.0 38.0 4.0 40.0 55 27.964012309184895 34.0 23.0 38.0 4.0 40.0 56 26.879470038049327 33.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.38407957711933705 1 2 0.6978891036538428 1 3 0.927410619941547 1 4 1.7285196822287503 1 5 1.4920911235783052 1 6 3.4623253357286643 1 7 1.1998542362551277 1 8 1.292850548175167 1 9 1.3582602594620852 1 10 1.17484765884128 1 11 -2.926241710332704 1 12 -12.31049446800197 1 13 -6.7323511736772055 1 14 -5.313844390634181 1 15 -4.722061595968267 1 16 -22.83369730031371 1 17 -3.9018200571711503 1 18 -13.608120403136066 1 19 -4.599359139757851 1 20 -4.662821045807256 1 21 -6.280260115941807 1 22 -2.6758976705893005 1 23 -3.4038185139832997 1 24 -3.624169548424952 1 25 -3.0251372245286134 1 26 2.4496824662102625 1 27 2.4942847274377726 1 28 2.486099588182306 1 29 1.6752386950933413 1 30 2.380273294950598 1 31 1.7449343027896518 1 32 1.890846071157405 1 33 1.6989174817622228 1 34 1.0737879134052122 1 35 1.3612140133033037 1 36 1.8057162128776838 1 37 1.6285812654409781 1 38 1.5262598129624223 1 39 2.0613047400080404 1 40 4.518340260924671 1 41 -10.351259537302397 1 42 -9.479727832894117 1 43 -18.11529872045262 1 44 -21.525478786952807 1 45 -11.521674315189983 1 46 -16.92973033448238 1 47 -22.598264724183586 1 48 -15.10316820609354 1 49 -19.81453149096712 1 50 -14.4596721340194 1 51 -11.10706293978103 1 52 -10.453996897150642 1 53 -10.35735733074792 1 54 -9.694145509372671 1 55 -10.081362360925116 1 56 -6.254194957554084 2 1 0.9717026432234164 2 2 1.3475374862136178 2 3 1.4434443752158117 2 4 2.3653691899643334 2 5 1.9375200968553798 2 6 3.6031129587947675 2 7 1.902948469729104 2 8 1.9595172148418314 2 9 1.8887806532736207 2 10 2.5641584886584425 2 11 1.5878229872762972 2 12 -1.5352483357938134 2 13 -0.6416338740991492 2 14 3.1239896459340315 2 15 3.215913087576034 2 16 2.6402830091096376 2 17 3.577504837343618 2 18 -0.7309052132626483 2 19 2.099796978385605 2 20 2.6181916124205884 2 21 3.607784891090539 2 22 3.33155661914347 2 23 3.2873207265230278 2 24 2.4647193404639367 2 25 2.571487248045223 2 26 2.664169104747536 2 27 2.0211482998709656 2 28 3.0178858047786505 2 29 2.3289658399597215 2 30 2.3659273033894195 2 31 0.7235559624239727 2 32 1.7797349600462908 2 33 0.8245152314106079 2 34 1.455503806513505 2 35 0.8907498782822074 2 36 1.450441951274307 2 37 1.1342071444845772 2 38 0.6638125555784598 2 39 2.1828237273498132 2 40 5.445203833357862 2 41 -7.641554895952186 2 42 -7.5146082829644385 2 43 -14.171416863912539 2 44 -15.036449251087829 2 45 -7.530816368635836 2 46 -11.176565777520356 2 47 -17.256211278332675 2 48 -9.516810962774272 2 49 -12.203982967760368 2 50 -9.099334581276782 2 51 -5.000593178880887 2 52 -4.578188177038122 2 53 -4.425289820199396 2 54 -3.8079289130294924 2 55 -4.155061376396283 2 56 -1.4527884877931854 3 1 1.5025939741147454 3 2 1.7564507951269235 3 3 1.8657886975601272 3 4 2.890155514750653 3 5 2.539107398442681 3 6 4.2296086852904935 3 7 2.060335527116159 3 8 2.0071362624608824 3 9 2.1893911538841166 3 10 2.816661541161494 3 11 3.9181038175571317 3 12 5.9924683919229125 3 13 4.664226931761661 3 14 5.2025000244444115 3 15 5.228000999663948 3 16 4.664703033529662 3 17 4.978970038808818 3 18 5.294369512012075 3 19 4.128002106590735 3 20 3.862147656376635 3 21 4.436112119417771 3 22 4.421056008642857 3 23 3.612595451797752 3 24 4.430775506520106 3 25 3.6535385300965046 3 26 3.1446330852115167 3 27 2.8509407296633995 3 28 3.2097050965979435 3 29 3.784155095148982 3 30 2.976183713645831 3 31 2.7428477817157884 3 32 1.7369999173112518 3 33 2.4598021666975427 3 34 2.6244903755000735 3 35 2.727746215278543 3 36 3.288537189369542 3 37 4.350934861212295 3 38 4.032310724076623 3 39 4.694911639437727 3 40 8.52334791150194 3 41 9.058811404414111 3 42 5.518602950246798 3 43 -0.12489671739239228 3 44 3.7983492837107065 3 45 5.506424168604703 3 46 3.0596979587433815 3 47 -1.7077375298589246 3 48 2.7272671813038727 3 49 5.648397984620583 3 50 3.4845482026059997 3 51 4.778039299751587 3 52 5.03438813553819 3 53 5.101816406906831 3 54 6.065209060108479 3 55 4.95800333666843 3 56 5.028041793037097 4 1 1.0980311456388776 4 2 1.286909466889945 4 3 1.4202559694187045 4 4 2.6861421368242375 4 5 2.149355845647655 4 6 3.799006678601529 4 7 1.7469098223861081 4 8 1.9450244612186438 4 9 1.5812927305683075 4 10 2.400631918610131 4 11 3.328161151527503 4 12 5.7804521472979715 4 13 5.4090907635820145 4 14 4.681477568639345 4 15 4.261565261489082 4 16 4.601635666114468 4 17 4.7086642576334725 4 18 4.569094786737352 4 19 3.699072340704447 4 20 4.111669873290154 4 21 3.0073017993031 4 22 3.128174976631392 4 23 3.180798987392592 4 24 3.4627869733141807 4 25 3.4946756538423287 4 26 2.516826109578453 4 27 1.619215932721211 4 28 1.4625717951168156 4 29 2.6968402360949923 4 30 2.8775215062879695 4 31 1.4356332571099628 4 32 2.640846071157405 4 33 1.6247567773043272 4 34 1.9202381060304177 4 35 1.046546979731481 4 36 2.4671086179409727 4 37 3.37816849714158 4 38 2.6210589323900493 4 39 3.2502150316976426 4 40 6.284575814034195 4 41 8.570039306946367 4 42 6.529353069692565 4 43 7.594525165072966 4 44 6.919347850361447 4 45 5.945029041992186 4 46 6.269086396392691 4 47 5.491856354517569 4 48 5.835604496162926 4 49 7.743118481514994 4 50 5.783998752056551 4 51 3.5940928114572763 4 52 3.99234322392806 4 53 3.45442032472814 4 54 3.5824092512217156 4 55 2.0577405559708666 4 56 2.2918975025449804 5 1 1.616147087667862 5 2 1.7967438354199672 5 3 2.2577300895015213 5 4 3.1804485550436965 5 5 2.6105359698712576 5 6 4.602319308001121 5 7 2.2013611681418013 5 8 2.2214219767465977 5 9 2.244336208829175 5 10 2.7205076950076474 5 11 3.7862356856890003 5 12 5.7726881721426935 5 13 5.775032792567522 5 14 5.6851007570451415 5 15 5.9230559447188895 5 16 5.172029040855669 5 17 5.59893340877219 5 18 5.866713834356403 5 19 4.737892216480844 5 20 4.639620183849161 5 21 4.104610287915939 5 22 4.5236201112069665 5 23 4.196844536046839 5 24 4.625830451575048 5 25 4.1452967718547455 5 26 3.553057993636422 5 27 3.6274975062201733 5 28 3.318679455572301 5 29 3.798807109800997 5 30 3.649260636722751 5 31 3.3719686608366715 5 32 3.688465118776456 5 33 2.790388247283623 5 34 3.440424441434139 5 35 3.0455117830441125 5 36 4.4075848084171625 5 37 4.475476985754419 5 38 4.970955412721317 5 39 5.009014203540289 5 40 8.961076849230881 5 41 10.722730818333527 5 42 7.936185367829214 5 43 8.407154564658889 5 44 8.049265034626458 5 45 7.750929663110199 5 46 7.34724374628917 5 47 7.202518880397488 5 48 6.87287157690827 5 49 11.172207508430109 5 50 7.343522561580361 5 51 6.866867138579426 5 52 7.071018172168227 5 53 7.767567322657744 5 54 8.921436166335589 5 55 6.7647798934449845 5 56 6.850386115381422 6 1 0.9191773906981595 6 2 1.2745576882338199 6 3 1.3199595267309618 6 4 1.8254702000653396 6 5 1.8296918140271003 6 6 3.6255877062695134 6 7 1.5888070555876936 6 8 1.4633050936297138 6 9 1.6079725724655418 6 10 2.06466353916349 6 11 3.2001967246500342 6 12 4.964246613701135 6 13 4.975790368325093 6 14 5.109343181287571 6 15 4.293185814848762 6 16 4.703414322240953 6 17 4.156292716131496 6 18 4.7622766049191725 6 19 2.905100008688642 6 20 3.9659188851478646 6 21 2.746926305231952 6 22 2.9191828817697356 6 23 3.000957090159396 6 24 3.432648633393228 6 25 2.4699720965300713 6 26 1.6704822360606641 6 27 1.9186230473457115 6 28 2.2348049966978394 6 29 1.4670971530910393 6 30 2.331078818540931 6 31 1.626838790706799 6 32 2.072664252975585 6 33 1.0674445243398978 6 34 1.2878270388367383 6 35 1.0687801813125049 6 36 1.6261995270318792 6 37 2.2768839121613453 6 38 2.1630549798208847 6 39 2.5214600909861815 6 40 6.226769489923523 6 41 2.1940511646538745 6 42 3.9848866665305103 6 43 6.027825560329887 6 44 4.725671961033385 6 45 3.7557997929803264 6 46 5.425706949752371 6 47 4.313990741869343 6 48 4.557936511973207 6 49 6.555865517088119 6 50 4.352180570238371 6 51 3.4572351039473936 6 52 4.427619903769955 6 53 3.9504677555581793 6 54 4.886263006162427 6 55 5.145191148856238 6 56 3.223221613216918 7 1 1.635086481607253 7 2 1.8768304155065465 7 3 2.1722322540036885 7 4 3.2686520182471597 7 5 2.4092372685725536 7 6 3.909678615360427 7 7 2.1342616010422333 7 8 2.3723960027206203 7 9 2.2102452997382684 7 10 2.791936266436217 7 11 3.7229239973773076 7 12 5.918792068246589 7 13 5.237154004688733 7 14 5.5979795449239305 7 15 5.100003996666942 7 16 5.430687049513679 7 17 5.679019988858769 7 18 5.841822059464626 7 19 4.6323727359613684 7 20 5.034100703329685 7 21 3.7696535779592253 7 22 4.6010010635879155 7 23 3.8532298174321227 7 24 4.114466815211415 7 25 3.7995175510755246 7 26 3.215936781515211 7 27 3.179986683709352 7 28 3.337077723970573 7 29 3.501188062181946 7 30 3.0697151821772977 7 31 2.7972933361613457 7 32 3.311300616611952 7 33 2.715171797067171 7 34 2.583281584291285 7 35 3.4210529085852386 7 36 3.1375631633955194 7 37 3.0382475485249785 7 38 3.4585095252754243 7 39 3.669187363713448 7 40 6.965405853559883 7 41 -0.3514033808006687 7 42 4.7462503028941505 7 43 7.1641891966935205 7 44 7.214308324669744 7 45 5.84670888388942 7 46 6.232525131570551 7 47 6.143536196414804 7 48 5.285209239245933 7 49 8.805865517088119 7 50 5.931726024783824 7 51 5.786780558492847 7 52 5.745801721951775 7 53 5.757285937376363 7 54 7.4771720970715165 7 55 5.099736603401695 7 56 5.711857976853281 8 1 1.2466691760212711 8 2 1.486359440698223 8 3 1.6960548169828797 8 4 2.9763464322668725 8 5 1.9349765894925994 8 6 3.9154288891829907 8 7 2.116736957011568 8 8 2.2326095843197464 8 9 2.3045771726845956 8 10 2.674466386918148 8 11 3.9084387837957095 8 12 5.836371476789857 8 13 5.541097049595635 8 14 5.35277143977607 8 15 5.207479352636277 8 16 4.464914869886073 8 17 5.014452628508273 8 18 4.990781533725304 8 19 3.93152388601613 8 20 4.384456672661557 8 21 3.3711048375430153 8 22 3.584434798527674 8 23 2.6343086783302567 8 24 3.797517198563 8 25 3.37550331230225 8 26 2.3381985558854197 8 27 2.640693145921837 8 28 2.84693533623782 8 29 3.064441074230139 8 30 3.2245618415420267 8 31 2.68393079508796 8 32 2.6378340229646327 8 33 2.539240800353042 8 34 2.7730406204358644 8 35 2.720067147358513 8 36 2.572530304687959 8 37 2.796188402851378 8 38 2.7393145636105842 8 39 3.9274020406028285 8 40 5.724989643264156 8 41 -1.281715538522466 8 42 4.736529601908384 8 43 6.061916469420794 8 44 5.327807777024624 8 45 4.002650833506067 8 46 5.516205306816996 8 47 5.233213085790485 8 48 5.46538448568624 8 49 7.49521381938823 8 50 5.335203571333661 8 51 5.686287677879488 8 52 5.057822532466563 8 53 5.517280460925104 8 54 6.382566401562208 8 55 5.8326226932154945 8 56 5.109995983425023 9 1 0.9030669188914899 9 2 0.8877625213753575 9 3 1.4970308731061053 9 4 2.6525703151401387 9 5 1.7199392248441328 9 6 3.7281481205387905 9 7 1.668208666634868 9 8 1.7232302950106089 9 9 1.700032411360823 9 10 2.136872975296974 9 11 3.769960821312864 9 12 5.5249485699727146 9 13 5.381572876955708 9 14 5.141851811897467 9 15 5.024773847069703 9 16 4.989087509812869 9 17 4.617167284601003 9 18 5.467828963952542 9 19 4.084185164039617 9 20 4.792875156724392 9 21 3.6058158334252823 9 22 4.387393468651204 9 23 3.5708650303204976 9 24 4.110007666764922 9 25 3.747014674205559 9 26 2.6901887953241896 9 27 3.5828923223744837 9 28 3.6759730059038276 9 29 3.3967576824351084 9 30 3.2157163329252825 9 31 2.225946961017499 9 32 3.106035944575126 9 33 2.3191706463191863 9 34 2.719357533658375 9 35 1.9814671778602673 9 36 2.944956719206793 9 37 3.1982015186055293 9 38 3.842715509164954 9 39 3.9980136007675355 9 40 6.29049215965885 9 41 3.9778543867482377 9 42 5.191158243055252 9 43 7.0998911529651 9 44 7.12009083330036 9 45 5.604907963291026 9 46 6.386725361720149 9 47 6.680216288474636 9 48 5.529743186311524 9 49 8.641164711564524 9 50 6.69222660015782 9 51 5.968886427307577 9 52 6.166256267406322 9 53 6.718592036340684 9 54 7.14129752860201 9 55 6.019903461859695 9 56 5.024286055104142 10 1 1.4347124885117424 10 2 1.41940809099561 10 3 1.598296695890916 10 4 2.7158614543806436 10 5 1.8844961868694483 10 6 4.082578500285628 10 7 1.427702337520948 10 8 1.81183788994732 10 9 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1.9265663603342063 115 29 2.188688062181946 115 30 1.977385636722751 115 31 1.1822365179795256 115 32 1.765846071157405 115 33 0.8543763425217179 115 34 2.755156584291285 115 35 1.1199165449488717 115 36 2.3108586179409727 115 37 1.9345543667067986 115 38 1.7908958889117912 115 39 3.0328237273498146 115 40 5.915689944468976 115 41 5.513653437381148 115 42 1.0076139392577836 115 43 -6.547458530579206 115 44 -7.866657584421162 115 45 1.779947520253053 115 46 -3.8328157775203557 115 47 -2.9701001672215632 115 48 -2.77444985166316 115 49 -13.809191301093701 115 50 -2.606626247943449 115 51 -1.316912623325333 115 52 -0.9743687325936783 115 53 -2.4742481535327308 115 54 -9.524248357473937 115 55 -2.694297487507395 115 56 -0.9316079322376289 116 1 0.5769314013933524 116 2 0.8968838914423714 116 3 1.2150130026667867 116 4 2.3268070984610603 116 5 1.873141011888059 116 6 3.7465770110823513 116 7 1.2545824566572108 116 8 1.381085842292812 116 9 1.097277385299762 116 10 2.174289207612688 116 11 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0.1719912617106374 119 35 0.8154810610779037 119 36 1.8945279727796844 119 37 2.24352614090035 119 38 2.358436211492439 119 39 2.7666946950917506 119 40 6.265992363823816 119 41 2.7046816631876 119 42 -1.3956118671938285 119 43 -13.63163191767598 119 44 -11.473008390872774 119 45 -5.593540382972755 119 46 -16.837856100101 119 47 -16.11929371560866 119 48 -13.48311920650187 119 49 -15.02441307528725 119 50 -13.369227054395061 119 51 -9.838586010422109 119 52 -9.65632437775497 119 53 -9.861848959984343 119 54 -12.449147551022325 119 55 -9.688753132668687 119 56 -7.132716803205369 120 1 -0.8308226093018405 120 2 -0.5549877663116405 120 3 -0.46413138235995177 120 4 0.5981974727926129 120 5 0.11378272311800686 120 6 1.909678615360427 120 7 -0.2066474898668531 120 8 -0.11624036091573942 120 9 -0.34657288207991854 120 10 -0.5489728244728767 120 11 -4.061166911713599 120 12 -13.444844295389775 120 13 -10.331027813493087 120 14 -13.845202273257886 120 15 -11.888632366969418 120 16 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120 54 -13.988736993837573 120 55 -14.138899760234668 120 56 -9.78814202314672 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 403.0 5 79.0 6 53.0 7 60.0 8 85.0 9 76.0 10 67.0 11 63.0 12 86.0 13 81.0 14 133.0 15 158.0 16 244.0 17 306.0 18 342.0 19 392.0 20 496.0 21 693.0 22 854.0 23 991.0 24 1171.0 25 1289.0 26 1424.0 27 1626.0 28 1799.0 29 2126.0 30 2503.0 31 3014.0 32 3353.0 33 3793.0 34 4533.0 35 5481.0 36 6756.0 37 8495.0 38 8062.0 39 4876.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.73926357117952 0.17584549850682918 0.05457274091591251 0.030318189397729168 8 99.67711128291418 0.23496596783240103 0.027286370457956254 0.060636378795458336 9 99.14047933057438 0.4684160261949156 0.2289023299528552 0.16220231327785103 10 57.30895750905756 30.542543999272365 5.601285491230463 6.547213000439614 11 37.89925265663135 30.889687267876365 16.54312004487092 14.66794003062137 12 37.683993511907474 13.87663528734064 22.161080540270135 26.27829066048176 13 25.123966942148762 14.769883994237624 27.043748578360756 33.062400485252866 14 23.233180729466696 18.34884637540551 30.66882939696207 27.749143498165722 15 26.046115730115062 17.462874861303217 28.60725631165357 27.88375309692815 16 31.21236481669675 19.197925040573953 24.94805017518846 24.64165996754084 17 29.881607470401867 20.774023375324024 24.518319765943577 24.826049388330528 18 28.57186168841997 22.318735125138325 23.95743326208559 25.151969924356116 19 29.596187990348575 23.97377725844879 21.557885791462432 24.8721489597402 20 29.06373292867982 23.855842185128985 22.643399089529588 24.437025796661608 21 28.69919808389043 23.492049054830446 23.702760471144664 24.10599239013446 22 27.650188730729003 21.403125805326905 25.6688950535874 25.277790410356694 23 28.047357011839253 22.336926038776962 25.141358558066912 24.47435839131687 24 29.314657328664335 21.196962117422345 24.8518198493186 24.63656070459472 25 28.373707667586334 21.87338932177182 24.66725282721402 25.085650183427827 26 25.371821889355513 20.241362058248306 28.28878545763277 26.098030594763415 27 23.708571081704505 18.5155396382178 29.558698555299262 28.21719072477844 28 24.84062898427539 20.745552789751684 29.095076194523706 25.318742031449215 29 26.965832056901075 20.622090113589834 29.35440331215214 23.05767451735695 30 25.263046180902997 18.36168470845083 33.46826768549683 22.907001425149335 31 26.72004610042006 18.3977071106865 33.030041096098145 21.852205692795295 32 27.697607016585486 17.453452906632677 33.632266581690715 21.216673495091122 33 28.613730581750264 16.790427163530893 34.836681700150336 19.75916055456851 34 23.246678395923073 19.46854334769156 39.313231814596854 17.97154644178851 35 21.839690604383104 21.337681049518466 39.95905058011678 16.86357776598165 36 22.536813211810557 22.119773737128646 37.76861133437467 17.574801716686128 37 24.427330173775673 21.749301251670918 35.44932555596063 18.374043018592783 38 23.611681184536568 22.05500068408811 36.268831425487605 18.06448670588772 39 25.112768251750378 20.094770894400316 35.37657761645126 19.415883237398052 40 18.14975417595505 14.912816441200722 31.867041255687546 35.07038812715669 41 15.5516476794862 15.196880495450724 30.574202920712594 38.677268904350484 42 20.42201138519924 18.70360531309298 36.47210626185958 24.402277039848197 43 18.509962460294542 15.807711597793212 37.52602702250862 28.15629891940362 44 18.353195769453503 15.747203511231675 37.10872016824652 28.790880551068305 45 22.74198696028815 18.53371631787717 37.468654538822776 21.2556421830119 46 20.81086418252799 20.832130759064878 36.981057556470354 21.375947501936775 47 19.13470724716801 19.381890420514686 37.908496732026144 23.57490560029116 48 21.162285696931043 19.786494161237908 39.53958058099099 19.511639560840052 49 19.507546882146666 16.337856380545816 33.86186918737613 30.292727549931392 50 20.456029025549164 19.460173363897194 38.275165849437556 21.808631761116086 51 19.724156807882974 19.988747300872845 38.65302150177914 21.63407438946504 52 20.34513113161729 19.645458960660513 38.284664885866924 21.72474502185527 53 19.791666666666664 19.765852769679302 39.376822157434404 21.06565840621963 54 18.427171187599725 19.49092014284629 37.15219208266849 24.929716586885494 55 19.994223958412498 20.418307011810484 36.560823237927316 23.026645791849703 56 20.0206633544525 21.14955102784995 35.537930929699016 23.29185468799854 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 5.5 11 11.0 12 47.0 13 150.0 14 217.0 15 257.0 16 297.0 17 349.0 18 401.0 19 461.0 20 521.0 21 554.5 22 710.5 23 833.0 24 965.0 25 1097.0 26 1315.5 27 1534.0 28 1746.0 29 2107.5 30 2257.0 31 2264.0 32 2271.0 33 2085.5 34 1900.0 35 1691.5 36 1483.0 37 1366.5 38 1169.5 39 1089.0 40 1042.5 41 996.0 42 1049.5 43 1103.0 44 1164.0 45 1225.0 46 1358.5 47 1580.5 48 1669.0 49 1657.0 50 1645.0 51 1744.0 52 1843.0 53 2113.0 54 2514.0 55 2645.0 56 3052.5 57 3460.0 58 3669.0 59 3878.0 60 4334.5 61 4791.0 62 4985.5 63 4707.0 64 4234.0 65 3755.5 66 3277.0 67 2974.0 68 2671.0 69 2274.5 70 1878.0 71 1622.0 72 1186.0 73 1006.0 74 848.0 75 690.0 76 547.5 77 405.0 78 286.5 79 140.5 80 113.0 81 75.0 82 37.0 83 23.0 84 9.0 85 7.0 86 5.0 87 4.0 88 2.0 89 1.0 90 2.0 91 3.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.03335000833750208 14 0.0015159094698864585 15 0.26680006670001666 16 0.057604559855685425 17 0.0 18 0.0 19 0.10611366289205208 20 0.10156593448239272 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0015159094698864585 26 0.012127275759091668 27 0.10762957236193854 28 0.12582048600057605 29 0.042445465156820836 30 0.013643185228978127 31 0.036381827277275 32 0.10005002501250625 33 0.17281367956705626 34 0.05305683144602604 35 0.04850910303636667 36 0.03941364621704792 37 0.20464777843467188 38 0.2819591613988813 39 0.18797277426592082 40 0.7170251792562948 41 0.8671002167750541 42 0.13946367122955416 43 0.25770460988069793 44 0.529052404990374 45 0.254672790940925 46 0.20616368790455833 47 0.036381827277275 48 0.17281367956705626 49 0.5714978701471948 50 0.14249549016932708 51 0.31076144132672395 52 0.11975684812103021 53 0.16826595115739687 54 0.24557733412160623 55 0.2698318856397896 56 0.22738642048296875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 65967.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.10675034486941 #Duplication Level Percentage of deduplicated Percentage of total 1 85.57255656016038 63.41504085376022 2 8.421634005645789 12.481998575045099 3 2.7349343370290065 6.080312883714584 4 1.2478010064231069 3.6988191065229583 5 0.6504929836763081 2.410296057119469 6 0.3763858773472978 1.67356405475465 7 0.22296772081986665 1.1566389255233678 8 0.14523585484596818 0.8610365788955083 9 0.12068894980157918 0.8049479285097093 >10 0.48071022378595096 5.48759228098898 >50 0.01636460336292599 0.7928206527506177 >100 0.010227877101828745 1.1369321024148438 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 198 0.3001500750375188 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 158 0.23951369624206043 No Hit CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 157 0.23799778677217395 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 120 0.18190913638637501 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 117 0.17736140797671565 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGTTCTTCC 86 0.1303682144102354 No Hit CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 79 0.11975684812103021 No Hit CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACCCTCCTCGTTCGCGCA 67 0.10156593448239272 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.015159094698864584 0.0 0.0 0.0 0.0 5 0.015159094698864584 0.0 0.0 0.0 0.0 6 0.015159094698864584 0.0 0.0 0.0 0.0 7 0.015159094698864584 0.0 0.0 0.0 0.0 8 0.015159094698864584 0.0 0.0 0.0 0.0 9 0.015159094698864584 0.0 0.0 0.0 0.0 10 0.015159094698864584 0.0 0.0 0.0 0.0 11 0.01970682310852396 0.0 0.0 0.0 0.0 12 0.01970682310852396 0.0 0.0 0.0 0.0 13 0.01970682310852396 0.0 0.0 0.0 0.0 14 0.021222732578410418 0.0 0.0 0.0 0.0 15 0.02728637045795625 0.0 0.0 0.0 0.0 16 0.02728637045795625 0.0 0.0 0.0 0.0 17 0.02880227992784271 0.0 0.0 0.0 0.0 18 0.03183409886761562 0.0 0.0 0.0 0.0 19 0.03941364621704792 0.0 0.0 0.0 0.0 20 0.04092955568693438 0.0 0.0 0.0 0.0 21 0.042445465156820836 0.0 0.0 0.0 0.0 22 0.042445465156820836 0.0 0.0 0.0 0.0 23 0.045477284096593754 0.0 0.0 0.0 0.0 24 0.04850910303636667 0.0 0.0 0.0 0.0 25 0.04850910303636667 0.0 0.0 0.0 0.0 26 0.05154092197613958 0.0 0.0 0.0 0.0 27 0.05154092197613958 0.0 0.0 0.0 0.0 28 0.0545727409159125 0.0 0.0 0.0 0.0 29 0.05608865038579896 0.0 0.0 0.0 0.0 30 0.06366819773523125 0.0 0.0 0.0 0.0 31 0.0651841072051177 0.0 0.0 0.0 0.0 32 0.06973183561477708 0.0 0.0 0.0 0.0 33 0.06973183561477708 0.0 0.0 0.0 0.0 34 0.06973183561477708 0.0 0.0 0.0 0.0 35 0.07579547349432292 0.0 0.0 0.0 0.0 36 0.07579547349432292 0.0 0.0 0.0 0.0 37 0.07579547349432292 0.0 0.0 0.0 0.0 38 0.07579547349432292 0.0 0.0 0.0 0.0 39 0.07731138296420938 0.0 0.0 0.0 0.0 40 0.07731138296420938 0.0 0.0 0.0 0.0 41 0.07882729243409584 0.0 0.0 0.0 0.0 42 0.08185911137386875 0.0 0.0 0.0 0.0 43 0.08337502084375521 0.0 0.0 0.0 0.0 44 0.08640683978352813 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCAAA 15 0.008066793 50.5077 41 CGAAGCC 15 0.008066793 50.5077 41 TTACTTT 15 0.008140824 50.391235 37 CGCTCTT 25 1.9996627E-5 50.275307 49 ACCGCTC 20 4.074385E-4 50.198315 47 TTACATT 15 0.00826533 50.198315 46 CTAGCAA 20 4.074385E-4 50.198315 46 CCTCCTG 15 0.008290401 50.15991 50 CTTCCTA 15 0.008315528 50.121555 42 TGATTGC 15 0.008340712 50.08327 44 TGCCTAG 15 0.008340712 50.08327 43 CTGATTG 15 0.008340712 50.08327 43 GTTGGGA 15 0.008416602 49.968746 32 GATTTAG 20 4.1834376E-4 49.930695 19 CAATTGT 20 4.1834376E-4 49.930695 27 TGTTGTT 20 4.1834376E-4 49.930695 18 GAGGCCA 20 4.1834376E-4 49.930695 17 TCCGGAA 15 0.0084420135 49.93069 17 GTAGGAT 15 0.0084420135 49.93069 33 GCCGATG 15 0.0084420135 49.93069 28 >>END_MODULE