FastQCFastQC Report
Fri 17 Jun 2016
SRR1526267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42117
Sequences flagged as poor quality0
Sequence length56
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA3330.7906546050288482No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.7407935038108127No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA2320.55084645155163No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA1490.3537763848327279No Hit
CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.30866395992117196No Hit
CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA1110.26355153500961603No Hit
CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA850.2018187430253817No Hit
CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.17095234703326448No Hit
CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA710.1685780088800247No Hit
CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA700.1662036707267849No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAA650.15433197996058598No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG560.1329629365814279No Hit
CTTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA530.12583992212170858No Hit
CTTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.1234655839684688No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.11871690766198921No Hit
CTTCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA470.11159389320226987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTCA150.00790866250.72815341
AATTTGC150.00829559550.11990450
AGTAGCA150.00841437249.9402628
CAGTAGC150.00841437249.9402627
CTGACTT150.00841437249.9402628
GTCTGAC150.00841437249.9402626
TAGCATA150.00841437249.9402630
CATATGC150.00841437249.9402633
GCATATG150.00841437249.9402632
GTAGCAT150.00841437249.9402629
TGGTTGA150.00845424549.8806714
GTGGTTT150.00845424549.8806715
TCTTAAT150.00845424549.8806714
TTAATCA150.00849425749.82121316
ACCTGGT204.2362575E-449.76190611
TCGTCTT204.2362575E-449.76190611
GTCTTAA204.2362575E-449.76190613
CGTCTTA204.2362575E-449.76190612
AATCAGC150.0085344149.761918
CCTGTAT150.0085344149.761913
Produced by FastQC (version 0.11.5)