##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526267_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42117 Sequences flagged as poor quality 0 Sequence length 56 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.752688937958546 39.0 38.0 40.0 38.0 40.0 2 38.49694897547309 39.0 38.0 40.0 36.0 40.0 3 38.32965310919581 39.0 38.0 40.0 35.0 40.0 4 38.23501199040767 39.0 38.0 40.0 35.0 40.0 5 38.33658617660327 39.0 38.0 40.0 35.0 40.0 6 37.57599069259444 39.0 38.0 40.0 33.0 40.0 7 38.354227509081845 39.0 38.0 40.0 35.0 40.0 8 38.28207137260489 39.0 38.0 40.0 35.0 40.0 9 38.212242087518106 39.0 38.0 40.0 35.0 40.0 10 37.78027874729919 39.0 38.0 40.0 35.0 40.0 11 36.367713749792244 38.0 35.0 40.0 30.0 40.0 12 34.67462069948002 38.0 33.0 40.0 23.0 40.0 13 34.54075551440036 38.0 33.0 40.0 23.0 40.0 14 34.57641807346202 38.0 33.0 40.0 23.0 40.0 15 34.34446897927203 38.0 33.0 40.0 21.0 40.0 16 33.73243583351141 38.0 32.0 40.0 19.0 40.0 17 33.470997459458175 38.0 31.0 40.0 18.0 40.0 18 32.89816463660755 38.0 30.0 39.0 17.0 40.0 19 33.93852838521262 38.0 32.0 40.0 21.0 40.0 20 34.223330246693735 38.0 32.0 40.0 23.0 40.0 21 34.891302799344686 38.0 34.0 40.0 23.0 40.0 22 34.419616781822064 38.0 33.0 40.0 20.0 40.0 23 35.19785359830947 38.0 35.0 40.0 26.0 40.0 24 34.655483533964905 38.0 33.0 40.0 23.0 40.0 25 35.11707861433625 38.0 34.0 40.0 26.0 40.0 26 35.499916898164635 38.0 35.0 40.0 27.0 40.0 27 35.417242443668826 38.0 35.0 40.0 27.0 40.0 28 35.514661538096256 38.0 35.0 40.0 27.0 40.0 29 35.1733979153311 38.0 35.0 40.0 26.0 40.0 30 35.132535555713844 38.0 35.0 40.0 26.0 40.0 31 35.364935774152954 38.0 35.0 40.0 28.0 40.0 32 35.02276990288957 38.0 35.0 40.0 26.0 40.0 33 35.1631407745091 38.0 35.0 40.0 27.0 40.0 34 35.138875038582995 38.0 35.0 40.0 27.0 40.0 35 34.809150699242586 38.0 35.0 40.0 26.0 40.0 36 33.47681458793361 38.0 33.0 39.0 21.0 40.0 37 33.21110240520455 38.0 32.0 39.0 18.0 40.0 38 32.90367310112306 37.0 32.0 39.0 15.0 40.0 39 32.28055179618681 36.0 31.0 39.0 10.0 40.0 40 29.089797468955528 35.0 21.0 39.0 4.0 40.0 41 25.660445900705177 31.0 11.0 38.0 4.0 40.0 42 29.626136714390864 35.0 27.0 38.0 4.0 40.0 43 28.276705368378565 34.0 23.0 38.0 4.0 40.0 44 28.231165562599426 34.0 22.0 38.0 4.0 40.0 45 29.261367143908636 35.0 27.0 38.0 4.0 40.0 46 29.09506849965572 35.0 25.0 39.0 4.0 40.0 47 28.624949545314244 35.0 24.0 38.0 4.0 40.0 48 28.650331220172376 35.0 24.0 38.0 4.0 40.0 49 26.201486335683928 33.0 10.0 38.0 4.0 40.0 50 28.280884203528267 35.0 23.0 38.0 4.0 40.0 51 28.48104090984638 35.0 22.0 39.0 4.0 40.0 52 28.278224944796637 35.0 21.0 39.0 4.0 40.0 53 27.881093145285753 35.0 17.0 39.0 4.0 40.0 54 26.952608210461335 35.0 7.0 39.0 4.0 40.0 55 27.24754849585678 35.0 4.0 39.0 4.0 40.0 56 26.445212147113992 34.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.27828000864778346 1 2 -0.052195225909365206 1 3 0.19358130861346723 1 4 -0.07588906361512926 1 5 -0.13520569848522257 1 6 0.44743572670388687 1 7 -0.04126756053427272 1 8 -0.2938255553339104 1 9 -0.1519705939428917 1 10 0.03862760472737392 1 11 -5.143357638085011 1 12 -14.863154640703378 1 13 -9.917860459874728 1 14 -6.823742164892849 1 15 -7.033100143516993 1 16 -23.959723053345513 1 17 -5.304195768374488 1 18 -14.016588458730702 1 19 -5.31309634131371 1 20 -6.6869655957844 1 21 -3.7064919307851447 1 22 -1.3662331545744735 1 23 -2.567202375268714 1 24 -3.1045485593735798 1 25 -3.387454333338855 1 26 2.039674402673853 1 27 1.7242178002914912 1 28 2.2131310962072277 1 29 2.409056591776917 1 30 2.5579625955270657 1 31 2.5038933334144673 1 32 2.110564227450112 1 33 1.4436184707970554 1 34 2.0966942876292336 1 35 1.3099408468210072 1 36 2.391305258647961 1 37 2.5663900246111737 1 38 1.732655838958138 1 39 2.551990807156148 1 40 5.117008114018102 1 41 -9.346726210391857 1 42 -11.496672826695526 1 43 -16.37397731231766 1 44 -20.380944161246568 1 45 -10.604998214735659 1 46 -16.870398971993794 1 47 -21.6269133856444 1 48 -14.213894455071761 1 49 -18.752084835394243 1 50 -13.586076198826976 1 51 -9.748476477798409 1 52 -9.798022628671895 1 53 -10.141729760101711 1 54 -9.73376652143584 1 55 -9.769458338717797 1 56 -5.109593855427242 2 1 -0.29918301868122654 2 2 -0.6642353596886252 2 3 0.33655789724222984 2 4 0.17912765879289338 2 5 0.3982391175682949 2 6 0.9583052919212776 2 7 0.11425083411455716 2 8 0.026408558378463454 2 9 0.44919997461897765 2 10 1.0862864676036281 2 11 1.259652395359808 2 12 -1.8221847410378302 2 13 0.3973569314296199 2 14 3.366893286612168 2 15 1.9869667461151153 2 16 2.9156950068551595 2 17 3.207509917244245 2 18 0.30197341417899537 2 19 2.6141611837699017 2 20 1.7954758757875098 2 21 2.1597288050008103 2 22 2.9606899223486067 2 23 1.3542023070055293 2 24 1.0074915744056838 2 25 1.7546861348885727 2 26 2.2018817605333822 2 27 2.378900074538983 2 28 1.7005892901871604 2 29 2.321263949636446 2 30 2.323012762751148 2 31 1.8391776143509233 2 32 2.524443826112325 2 33 1.3474646246432087 2 34 1.5649217123783998 2 35 2.242215094312641 2 36 1.7207366967750488 2 37 1.6458214627382617 2 38 2.550381591466504 2 39 3.0820911416043053 2 40 4.040085037095025 2 41 -6.268967013067442 2 42 -7.568579181210577 2 43 -10.608091024692243 2 44 -13.30736556592884 2 45 -6.0999814923276325 2 46 -9.80350933988677 2 47 -16.442130776948748 2 48 -8.009044956744003 2 49 -12.03803801265177 2 50 -7.666343757355403 2 51 -3.998476477798409 2 52 -3.648357076832429 2 53 -3.6735023353525484 2 54 -2.864201304044535 2 55 -3.3029031547713075 2 56 -2.406416597902158 3 1 0.5942328874465375 3 2 0.5417620327494461 3 3 0.9835592010231977 3 4 0.7980976718307033 3 5 0.7704685830180935 3 6 1.5388137664975474 3 7 0.9252025942188595 3 8 0.848768401924751 3 9 0.9254059720114185 3 10 1.2143829473950234 3 11 2.5556106743689284 3 12 4.345025167697507 3 13 3.2321026941414814 3 14 3.2267368328963926 3 15 3.89505018809686 3 16 5.347572451444464 3 17 6.020417348795739 3 18 6.2612302590290625 3 19 5.073418028619969 3 20 4.948996084392462 3 21 4.2894550109982035 3 22 5.1083431166119695 3 23 3.805310520825607 3 24 4.233697571798125 3 25 4.0336952613553265 3 26 3.939821786609002 3 27 4.143893555634158 3 28 3.511215105050262 3 29 3.885475162152744 3 30 2.620600767966266 3 31 1.5989559715869106 3 32 2.036503800036705 3 33 1.6809066063902733 3 34 1.0303715168112504 3 35 1.5365436471157707 3 36 2.599485067048846 3 37 3.113552883859512 3 38 3.2772394793413397 3 39 3.9993010503396427 3 40 7.167855571645223 3 41 8.852610561899962 3 42 5.326466451123196 3 43 -0.34081592690866813 3 44 1.1267934953488634 3 45 4.565598690201707 3 46 2.8223055232162295 3 47 -1.1158595905080695 3 48 2.9202249259157114 3 49 5.125586498430369 3 50 3.464034339124389 3 51 5.8362692849134525 3 52 5.578174865801209 3 53 5.402116960605731 3 54 6.830713950192752 3 55 6.350291108592447 3 56 7.515682489451166 4 1 0.744264274766067 4 2 1.0693828739296052 4 3 1.173452484136817 4 4 1.245366975032212 4 5 1.1916864110155885 4 6 1.5601571437731323 4 7 1.010262230125953 4 8 1.1667219486918583 4 9 1.1507669261859306 4 10 1.1553747866919437 4 11 3.275321911029323 4 12 4.936362267509182 4 13 5.258468042540734 4 14 4.346950266669147 4 15 4.288961048109414 4 16 5.979085320245474 4 17 6.5379942477285695 4 18 6.5961329583385435 4 19 5.630542950151671 4 20 4.994193824505459 4 21 3.948902594174598 4 22 4.596729808388488 4 23 3.566453019256244 4 24 4.959058525972637 4 25 4.288874169076607 4 26 2.8899159485675767 4 27 2.9166493622882683 4 28 2.308139147737016 4 29 2.4259648043373048 4 30 2.225434415172799 4 31 2.1133941385674504 4 32 2.197520749189245 4 33 0.8510258782044602 4 34 0.8398504873071744 4 35 1.408169510267058 4 36 2.4685999446382993 4 37 3.125166192082993 4 38 2.6714642125491253 4 39 3.998045557558722 4 40 7.098489595499583 4 41 10.067927573827145 4 42 5.343101730470337 4 43 7.5785186619174425 4 44 6.349329590766313 4 45 5.105146712800572 4 46 4.598200061822634 4 47 4.567128482310512 4 48 4.302208604509559 4 49 6.843728369114615 4 50 4.2367901457784996 4 51 2.3441161147941827 4 52 2.3138936354182817 4 53 2.305130143279932 4 54 3.117280177436946 4 55 2.348093996225842 4 56 2.6512757097901485 5 1 0.6380448477919174 5 2 0.700060721588585 5 3 0.8572400452828717 5 4 0.8543816500846191 5 5 0.8779198142301183 5 6 1.1988713296571234 5 7 0.919766073592065 5 8 0.928077644009818 5 9 1.0235831386247867 5 10 0.5837465546863569 5 11 2.61669158258767 5 12 4.781225999165365 5 13 4.373772025769242 5 14 4.5548468424902495 5 15 5.395529881093346 5 16 5.127233468393619 5 17 4.800398161075883 5 18 6.036733936675368 5 19 4.792317932681371 5 20 3.4721812458891037 5 21 4.042892810806322 5 22 4.0488611850481675 5 23 3.33968851890684 5 24 4.256403040298281 5 25 2.7721026806069986 5 26 2.5429557808526866 5 27 3.115111975845224 5 28 2.9157561987502945 5 29 2.629319102031225 5 30 2.8016049253055755 5 31 2.7335898059329224 5 32 2.5843132020194304 5 33 2.31154299909894 5 34 2.355923163757204 5 35 2.741852249610176 5 36 4.545119860780858 5 37 4.0409593437251985 5 38 3.4988576437161356 5 39 4.058143391241462 5 40 6.305687359301121 5 41 9.195111361388289 5 42 6.3581261888910205 5 43 6.553012023203259 5 44 6.13095083465171 5 45 5.164895140473529 5 46 5.533210544002923 5 47 5.8173031853154065 5 48 6.58348044238517 5 49 7.279088257304693 5 50 5.246573514052436 5 51 5.279825408994046 5 52 5.794676304880198 5 53 5.230706663196074 5 54 6.286742092181882 5 55 5.604208601397051 5 56 4.990898351299581 6 1 0.5142447815886726 6 2 0.08205343923174979 6 3 0.7397292872557273 6 4 0.16057165339478274 6 5 0.663421303803112 6 6 1.0416386252546133 6 7 0.9118216843169904 6 8 0.7622385178926336 6 9 0.6985252107863218 6 10 0.8200921356198236 6 11 3.200273178085851 6 12 5.000689752889301 6 13 4.638585001605058 6 14 5.030186134115539 6 15 4.939058513996358 6 16 6.179865047340989 6 17 6.384972805233446 6 18 6.454807422276161 6 19 5.758223262042506 6 20 5.152088561347561 6 21 4.072684270587857 6 22 4.949556319109739 6 23 4.076199607950201 6 24 4.673483477239692 6 25 3.046184110597075 6 26 3.313892556754709 6 27 3.1876045279802767 6 28 3.2623301808754164 6 29 3.5429238686647864 6 30 2.793660536030501 6 31 2.1377606103023368 6 32 3.17120495971556 6 33 2.075197418165473 6 34 1.5932617933500595 6 35 2.038773798766087 6 36 2.272693511889763 6 37 2.2547958217126194 6 38 2.5213667466621814 6 39 2.584790196934943 6 40 5.204727412263718 6 41 3.835958762910966 6 42 4.336279118384567 6 43 5.772475776927195 6 44 5.6251580508053 6 45 4.534971274204086 6 46 4.726855032582865 6 47 5.290325363402129 6 48 4.833397688330219 6 49 6.6643911371458024 6 50 5.525289171119631 6 51 5.628716504657728 6 52 4.692061276743818 6 53 4.77589037719806 6 54 5.767377643323886 6 55 4.65121290460791 6 56 4.896889744877868 7 1 0.6738939043956904 7 2 0.8634569480036802 7 3 1.312711743396079 7 4 1.067589197254435 7 5 1.1213160406452118 7 6 1.3483052919212781 7 7 0.926558526422248 7 8 1.0348700968400024 7 9 1.0115076669266685 7 10 1.2116710829882393 7 11 3.002729318436728 7 12 4.178584489731406 7 13 4.82735693142962 7 14 4.788431748150629 7 15 5.24081289996127 7 16 6.1472334683936225 7 17 5.910586840321166 7 18 6.464281106486688 7 19 4.956468876077594 7 20 4.920860491172121 7 21 3.8074211126931203 7 22 5.1337668454255265 7 23 4.164971537774761 7 24 4.0197992667133775 7 25 4.057763057965495 7 26 3.4988048374564613 7 27 3.7581308437697487 7 28 3.4844354440333163 7 29 4.388187026559528 7 30 4.248397378135763 7 31 3.1830237681970743 7 32 3.6775207491892417 7 33 3.9236184707970523 7 34 3.347998635455319 7 35 3.282984325081877 7 36 4.53304438908274 7 37 4.028129155045953 7 38 3.8396123606972736 7 39 3.652860372373542 7 40 7.797008114018102 7 41 4.967186833086405 7 42 6.806805434174038 7 43 7.970370513769296 7 44 7.683403664840387 7 45 6.458480046133911 7 46 6.722644506267077 7 47 6.327869223051255 7 48 6.734801197102154 7 49 9.50965429504054 7 50 6.9782716272599785 7 51 4.251523522201591 7 52 4.0524121539368 7 53 3.949574587724374 7 54 4.875798695955464 7 55 4.934019922151769 7 56 5.371275709790147 8 1 0.44796797846976943 8 2 0.4027162072629409 8 3 0.9512302619145956 8 4 0.6527743824396168 8 5 0.506501225830398 8 6 1.2083052919212776 8 7 0.8991511190148458 8 8 0.7778330598029655 8 9 0.891507666926671 8 10 1.2109303422475008 8 11 2.756803392510804 8 12 4.21414004528696 8 13 4.406616190688879 8 14 4.976579896298773 8 15 4.640812899961269 8 16 4.571677912838062 8 17 5.167623877358203 8 18 5.614651476857063 8 19 4.723135542744259 8 20 4.531230861542497 8 21 4.115569260841262 8 22 4.31895203061071 8 23 3.677564130367358 8 24 4.40350297041708 8 25 3.5110963912988282 8 26 2.463990022641646 8 27 3.6388715845104898 8 28 2.215546555144428 8 29 2.9074462858187857 8 30 3.688397378135761 8 31 3.1133941385674504 8 32 3.901224452892947 8 33 1.5917666189452007 8 34 2.9694801169368006 8 35 2.6859472880448365 8 36 4.098229574267926 8 37 3.49553656245336 8 38 4.189982731067644 8 39 5.572119631632798 8 40 6.4318229288329185 8 41 1.5123720182715914 8 42 5.565323952692559 8 43 5.393333476732259 8 44 5.941922183358908 8 45 5.4014430090968695 8 46 4.561163024785596 8 47 5.4004618156438475 8 48 5.005912308213265 8 49 6.640024665410909 8 50 4.959012368000721 8 51 5.4922642629423315 8 52 4.758338079862728 8 53 4.675500513650302 8 54 5.450613510770278 8 55 4.088834736966582 8 56 5.61423867275311 9 1 -0.8861060956043048 9 2 -0.21654305199631807 9 3 0.17271174339607853 9 4 0.3275891972544329 9 5 0.2613160406452124 9 6 0.6283052919212793 9 7 -0.6334414735777472 9 8 -0.325129903159997 9 9 0.15150766692666906 9 10 0.37167108298824303 9 11 2.222729318436727 9 12 3.8185844897314 9 13 4.187356931429619 9 14 4.148431748150628 9 15 4.040812899961267 9 16 4.527233468393618 9 17 4.230586840321166 9 18 4.744281106486689 9 19 3.9564688760775937 9 20 3.660860491172123 9 21 2.547421112693115 9 22 3.773766845425527 9 23 3.124971537774762 9 24 3.8197992667133747 9 25 2.4777630579654897 9 26 2.118804837456466 9 27 2.5181308437697467 9 28 1.924435444033314 9 29 2.368187026559525 9 30 2.108397378135763 9 31 0.7430237681970766 9 32 1.5775207491892473 9 33 0.9036184707970563 9 34 1.1479986354553162 9 35 0.9229843250818774 9 36 2.7730443890827416 9 37 3.3681291550459562 9 38 3.2596123606972682 9 39 3.59286037237354 9 40 4.597008114018099 9 41 3.7871868330864054 9 42 4.586805434174039 9 43 5.090370513769294 9 44 4.923403664840389 9 45 4.418480046133904 9 46 5.662644506267075 9 47 5.647869223051256 9 48 5.094801197102154 9 49 7.389654295040536 9 50 5.278271627259983 9 51 4.7315235222015914 9 52 5.612412153936802 9 53 5.709574587724376 9 54 6.375798695955467 9 55 5.23401992215177 9 56 4.991275709790148 10 1 0.3330775778650832 10 2 -0.2953185622004 10 3 0.3816913352328086 10 4 0.008405523785043556 10 5 0.2090711426860281 10 6 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22 2.2766239882826724 116 23 1.5373524901557118 116 24 2.699799266713377 116 25 1.8206202008226384 116 26 1.2788048374564625 116 27 0.8743213199602238 116 28 0.6044354440333137 116 29 0.5053298837023803 116 30 0.7122069019452866 116 31 -2.042690517517208 116 32 -2.8500982984298027 116 33 -2.937333910155324 116 34 -2.988191840735155 116 35 -2.2293966272990744 116 36 -0.9679079918696374 116 37 -1.6499660830492822 116 38 -0.6803876393027295 116 39 0.2890508485640133 116 40 2.855103352113339 116 41 0.9224249283244994 116 42 -4.072242184873581 116 43 -10.23629615289737 116 44 -10.648024906588184 116 45 -2.3948532871994246 116 46 -5.81449835087578 116 47 -5.168321253139222 116 48 -6.390913088612134 116 49 -12.135107609721366 116 50 -6.257918848930494 116 51 -4.7246669539888835 116 52 -4.691397369872725 116 53 -4.84566350751372 116 54 -11.483248923092154 116 55 -3.559313411181563 116 56 -2.1820576235431837 117 1 -1.3483283178265282 117 2 -0.8565430519963186 117 3 -0.5672882566039235 117 4 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0.6734834772396923 118 25 0.15144726849180756 118 26 1.989331153245935 118 27 2.1612887385065918 118 28 2.3412775492964712 118 29 1.9902922897174236 118 30 2.0041868518199735 118 31 0.558813241881289 118 32 -5.591952935021283 118 33 0.2594079444812678 118 34 -0.14358031191309806 118 35 0.06508958823977196 118 36 1.5972549153985298 118 37 2.16707652346701 118 38 1.9775070975393731 118 39 1.207597214478799 118 40 3.9064817982286293 118 41 1.6956078857179833 118 42 -4.45319456582596 118 43 -13.438050538862282 118 44 -13.418701598317506 118 45 -3.2457304801818836 118 46 -9.641566020048714 118 47 -11.902657092738222 118 48 -10.23677775026627 118 49 -13.861924652327883 118 50 -7.018570478003177 118 51 -4.5116343725352515 118 52 -4.2991667934316204 118 53 -6.72410962280194 118 54 -11.70630656720243 118 55 -5.304927446269282 118 56 -2.927671658630903 119 1 -1.292772762270971 119 2 -1.2136859091391727 119 3 -1.1625263518420184 119 4 -0.6276488979836614 119 5 -1.5358268164976394 119 6 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26 -0.23548087682925 120 27 -0.05901201337310624 120 28 -0.3812788416809738 120 29 -0.9518129734404752 120 30 -0.9687454790070973 120 31 -1.3569762318029248 120 32 -8.902479250810757 120 33 -1.070667243488657 120 34 -0.6548585074018263 120 35 -0.9055871034895517 120 36 1.64161581765417 120 37 1.8452720121888149 120 38 1.4767552178401289 120 39 1.3557175152306833 120 40 4.059865256875245 120 41 -1.58709888119931 120 42 -3.8531945658259588 120 43 -11.783915200516416 120 44 -10.27659633515961 120 45 -9.204377096723235 120 46 -14.671641208018636 120 47 -13.83498791980589 120 48 -10.57662737432642 120 49 -16.45891713353089 120 50 -10.167442658454306 120 51 -8.019905049226981 120 52 -7.710444988920344 120 53 -9.893282555132767 120 54 -12.607058446901677 120 55 -8.997408649276803 120 56 -6.148724290209852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 33.0 5 8.0 6 20.0 7 27.0 8 80.0 9 196.0 10 102.0 11 99.0 12 91.0 13 56.0 14 63.0 15 83.0 16 130.0 17 172.0 18 227.0 19 291.0 20 396.0 21 462.0 22 600.0 23 722.0 24 873.0 25 1030.0 26 1103.0 27 1065.0 28 1177.0 29 1308.0 30 1436.0 31 1687.0 32 1949.0 33 2160.0 34 2539.0 35 3070.0 36 3796.0 37 5001.0 38 5368.0 39 4676.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.11437186884156 0.6315739487617826 0.1400859510411473 0.11396823135550965 8 98.16701094569889 1.2702709119832847 0.2398081534772182 0.3229099888406107 9 91.05586817674573 5.805256784671273 1.7997483201557567 1.3391267184272384 10 29.496402877697843 47.02139278676069 13.897001210912457 9.58520312462901 11 25.431535959351333 45.02220006173279 16.102761355272218 13.44350262364366 12 31.118075836360614 29.62461713797279 17.672198874563716 21.58510815110288 13 19.772495191051796 30.22631741432948 21.952551711035646 28.048635683583083 14 20.597872542501662 34.970082628929624 21.317314084908347 23.114730743660367 15 21.813725490196077 32.27917380544451 22.09927660384542 23.80782410051399 16 21.10614843675758 36.486743324147106 21.40074123348855 21.006367005606766 17 21.466391243440892 38.07726096350642 18.564950020181875 21.891397772870814 18 19.382642763860858 40.01662115635759 19.261545767541254 21.339190312240294 19 19.892575339861203 42.59435307538739 18.40241467820135 19.11065690655005 20 20.75965012359764 40.41642897889333 17.472428218292453 21.35149267921658 21 19.654771232518936 38.36930455635492 20.248355770828883 21.72756844029727 22 20.086900776408577 38.946268727592184 19.550300353776386 21.416530142222857 23 20.687608329178243 39.8603889165895 20.17237694992521 19.27962580430705 24 19.007028207807007 40.006173425776424 23.190711368601008 17.796086997815557 25 20.48913688709486 40.02137005817405 22.673631722664133 16.81586133206696 26 19.749715045592705 39.18835486322189 24.98812689969605 16.07380319148936 27 19.197128868184627 37.296192422873986 26.360697818129964 17.145980890811426 28 18.91583452211127 38.162149310508795 28.371374227294343 14.550641940085592 29 17.965224001140196 37.816523350277926 29.923036723834862 14.295215924747017 30 15.246146904462229 39.53786601439122 31.76280605096298 13.453181030183572 31 13.978290301893068 39.42661694496567 34.14170684781834 12.453385905322914 32 14.079954366116842 36.9872130056567 36.22902505110044 12.703807577126017 33 13.018764715675315 37.12750017837182 38.05503365281708 11.798701453135777 34 11.753385602280826 37.949631741506295 39.82893799002139 10.468044666191496 35 11.477707308962207 38.11254424095584 40.54015534810803 9.86959310197392 36 11.851200798156638 37.16416846806186 40.30215929876238 10.682471435019123 37 12.22158766419189 36.07938320959452 41.276413478012564 10.422615648201027 38 13.011170124086027 35.93255055136114 40.86264796246457 10.193631362088265 39 12.681719764924217 35.042470675010115 40.88129624782888 11.39451331223679 40 9.924905534031664 26.13239584828048 36.41124982063424 27.53144879705362 41 9.164530272899347 23.34619162853104 34.358003689771664 33.13127440879795 42 12.7500178371822 34.14512331438628 42.10764144885486 10.997217399576664 43 11.063769012877579 28.38304253647854 41.12732379614863 19.42586465449525 44 11.565454024634775 29.590375250644513 39.84054234698749 19.00362837773322 45 12.961199714353725 34.103784813139725 42.337538681266366 10.597476791240181 46 12.159428911362284 33.82748364069006 42.21296847114813 11.800118976799524 47 12.255321170657545 32.90098821740783 41.923223109084 12.920467502850627 48 12.780434420574311 34.420574310660676 42.288202126900295 10.510789141864725 49 10.050383246973423 27.431409536999453 38.105016834212854 24.413190381814275 50 11.284453851459011 35.2945373255012 42.91421912530619 10.506789697733597 51 12.057464144470387 35.274217372659265 42.32858436174775 10.3397341211226 52 11.100806163943783 34.82200185489049 42.86223871013769 11.214953271028037 53 11.341948073582255 35.70357678303706 42.24792365721901 10.70655148616168 54 10.031661389768372 32.889756469159906 40.51229556978599 16.566286571285737 55 11.779412815201086 34.781055789699266 43.07688644426983 10.362644950829822 56 11.648498119674395 35.30727852620555 42.49297853096587 10.551244823154187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 4.0 5 8.0 6 13.5 7 19.0 8 63.0 9 107.0 10 404.0 11 701.0 12 1027.0 13 1254.5 14 1156.0 15 1015.5 16 875.0 17 853.5 18 832.0 19 875.0 20 918.0 21 996.5 22 1187.5 23 1300.0 24 1565.0 25 1830.0 26 2128.5 27 2427.0 28 2610.0 29 2811.5 30 2830.0 31 2794.0 32 2758.0 33 2490.0 34 2222.0 35 1926.0 36 1630.0 37 1413.0 38 1053.0 39 910.0 40 805.5 41 701.0 42 707.0 43 713.0 44 696.5 45 680.0 46 721.0 47 782.5 48 803.0 49 843.0 50 883.0 51 902.5 52 922.0 53 963.0 54 1019.5 55 1035.0 56 1041.0 57 1047.0 58 1069.0 59 1091.0 60 1112.0 61 1133.0 62 1078.0 63 972.0 64 921.0 65 835.5 66 750.0 67 619.0 68 488.0 69 444.5 70 401.0 71 336.0 72 246.5 73 222.0 74 183.0 75 144.0 76 130.0 77 116.0 78 74.5 79 25.0 80 17.0 81 15.0 82 13.0 83 8.0 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.018994705225918274 14 0.0023743381532397843 15 0.22081344825129998 16 0.059358453830994605 17 0.0 18 0.004748676306479569 19 0.09734786428283115 20 0.1068452168957903 21 0.0 22 0.0 23 0.0 24 0.0023743381532397843 25 0.004748676306479569 26 0.011871690766198923 27 0.10209654058931072 28 0.13533727473466772 29 0.045112424911555905 30 0.018994705225918274 31 0.03798941045183655 32 0.10209654058931072 33 0.1662036707267849 34 0.06410713013747418 35 0.04273808675831612 36 0.04748676306479569 37 0.22081344825129998 38 0.30866395992117196 39 0.20894175748510102 40 0.7194244604316548 41 0.9022484982311181 42 0.1662036707267849 43 0.25167984424341716 44 0.5342260844789515 45 0.25405418239665695 46 0.2184391100980602 47 0.049861101218035477 48 0.1994444048721419 49 0.5650924804710686 50 0.16145499442030534 51 0.3395303559132892 52 0.15670631811382577 53 0.2279364627110193 54 0.2611771968563763 55 0.2849205783887741 56 0.2445568297836978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 42117.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.56371536434219 #Duplication Level Percentage of deduplicated Percentage of total 1 87.98242262132212 65.60296317401524 2 6.874920392306713 10.252392145689388 3 2.2672271048274104 5.07158629532018 4 0.9839510890332442 2.9346819574043734 5 0.5285950834288625 1.9707006671890208 6 0.30569354222392053 1.3676187762661158 7 0.2483760030569354 1.2963886316689222 8 0.17513692523245447 1.0447087874255052 9 0.13374092472296523 0.8974998219246385 >10 0.4553560056043816 5.309020110644158 >50 0.02547446185199338 1.244153192297647 >100 0.019105846388995033 3.008286440154807 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 333 0.7906546050288482 No Hit CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 312 0.7407935038108127 No Hit CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 232 0.55084645155163 No Hit CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 149 0.3537763848327279 No Hit CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.30866395992117196 No Hit CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 111 0.26355153500961603 No Hit CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 85 0.2018187430253817 No Hit CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.17095234703326448 No Hit CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 71 0.1685780088800247 No Hit CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 70 0.1662036707267849 No Hit CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAA 65 0.15433197996058598 No Hit CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 56 0.1329629365814279 No Hit CTTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 53 0.12583992212170858 No Hit CTTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52 0.1234655839684688 No Hit CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 0.11871690766198921 No Hit CTTCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 47 0.11159389320226987 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0023743381532397843 0.0 0.0 0.0 0.0 8 0.0023743381532397843 0.0 0.0 0.0 0.0 9 0.0023743381532397843 0.0 0.0 0.0 0.0 10 0.007123014459719353 0.0 0.0 0.0 0.0 11 0.016620367072678492 0.0 0.0 0.0 0.0 12 0.026117719685637628 0.0 0.0 0.0 0.0 13 0.0308663959921172 0.0 0.0 0.0 0.0 14 0.03798941045183655 0.0 0.0 0.0 0.0 15 0.052235439371275255 0.0 0.0 0.0 0.0 16 0.052235439371275255 0.0 0.0 0.0 0.0 17 0.05935845383099461 0.0 0.0 0.0 0.0 18 0.06885580644395375 0.0 0.0 0.0 0.0 19 0.09497352612959138 0.0 0.0 0.0 0.0 20 0.10209654058931072 0.0 0.0 0.0 0.0 21 0.11634256950874944 0.0 0.0 0.0 0.0 22 0.14246028919438705 0.0 0.0 0.0 0.0 23 0.17332668518650426 0.0 0.0 0.0 0.0 24 0.20419308117862145 0.0 0.0 0.0 0.0 25 0.22081344825129995 0.0 0.0 0.0 0.0 26 0.2445568297836978 0.0 0.0 0.0 0.0 27 0.2659258731628559 0.0 0.0 0.0 0.0 28 0.2777975639290548 0.0 0.0 0.0 0.0 29 0.29204359284849346 0.0 0.0 0.0 0.0 30 0.3015409454614526 0.0 0.0 0.0 0.0 31 0.3062896217679322 0.0 0.0 0.0 0.0 32 0.31103829807441175 0.0 0.0 0.0 0.0 33 0.3157869743808913 0.0 0.0 0.0 0.0 34 0.3205356506873709 0.0 0.0 0.0 0.0 35 0.32765866514709024 0.0 0.0 0.0 0.0 36 0.33003300330033003 0.0 0.0 0.0 0.0 37 0.3371560177600494 0.0 0.0 0.0 0.0 38 0.3395303559132892 0.0 0.0 0.0 0.0 39 0.3395303559132892 0.0 0.0 0.0 0.0 40 0.34190469406652896 0.0 0.0 0.0 0.0 41 0.34665337037300853 0.0 0.0 0.0 0.0 42 0.3537763848327279 0.0 0.0 0.0 0.0 43 0.3703967519054064 0.0 0.0 0.0 0.0 44 0.37514542821188596 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCTCA 15 0.007908662 50.728153 41 AATTTGC 15 0.008295595 50.119904 50 AGTAGCA 15 0.008414372 49.94026 28 CAGTAGC 15 0.008414372 49.94026 27 CTGACTT 15 0.008414372 49.94026 28 GTCTGAC 15 0.008414372 49.94026 26 TAGCATA 15 0.008414372 49.94026 30 CATATGC 15 0.008414372 49.94026 33 GCATATG 15 0.008414372 49.94026 32 GTAGCAT 15 0.008414372 49.94026 29 TGGTTGA 15 0.008454245 49.88067 14 GTGGTTT 15 0.008454245 49.88067 15 TCTTAAT 15 0.008454245 49.88067 14 TTAATCA 15 0.008494257 49.821213 16 ACCTGGT 20 4.2362575E-4 49.761906 11 TCGTCTT 20 4.2362575E-4 49.761906 11 GTCTTAA 20 4.2362575E-4 49.761906 13 CGTCTTA 20 4.2362575E-4 49.761906 12 AATCAGC 15 0.00853441 49.7619 18 CCTGTAT 15 0.00853441 49.7619 13 >>END_MODULE