FastQCFastQC Report
Fri 17 Jun 2016
SRR1526266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31357
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG960.3061517364543802No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.22004656057658575No Hit
GTTGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCG640.20410115763625347Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT560.1785885129317218No Hit
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.14669770705105717No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA450.1435086264629907No Hit
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA380.12118506234652549No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC340.10842873999425966No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG340.10842873999425966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCTG150.008142007550.32689341
CGTTGTA150.008142007550.32689338
AAACCAC203.9904309E-450.3268940
AACCACC203.9904309E-450.3268941
CGCCTGG150.00824639850.1653346
ACCACAA150.00824639850.1653345
CCGTTGT204.0544514E-450.1653337
CAACAAC150.00824639850.1653343
AAGATTA204.0544514E-450.1653348
CACCACA150.00824639850.1653344
ATGTCAG150.00824639850.1653344
AGATTAA204.0544514E-450.1653349
AAAGATT150.00824639850.1653347
CCTGGTC150.00824639850.1653348
TGGTTGA451.2551027E-1050.08493414
GGTTGAT505.456968E-1250.08493415
CATTGGC150.00829896350.08493412
GACAGTG204.0867663E-450.0849349
GTACCTG451.2551027E-1050.0849349
GATACAA150.00829896350.0849349
Produced by FastQC (version 0.11.5)