##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526266_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31357 Sequences flagged as poor quality 0 Sequence length 56 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.25420161367478 39.0 38.0 40.0 35.0 40.0 2 38.196606818254295 39.0 38.0 40.0 35.0 40.0 3 38.000382689670566 39.0 38.0 40.0 35.0 40.0 4 37.628472111490254 39.0 38.0 39.0 35.0 40.0 5 37.897375386676025 39.0 38.0 39.0 35.0 40.0 6 38.102752176547504 39.0 38.0 40.0 35.0 40.0 7 37.843256689096535 39.0 38.0 39.0 35.0 40.0 8 37.866823994642345 39.0 38.0 40.0 35.0 40.0 9 37.889338903594094 39.0 38.0 40.0 35.0 40.0 10 37.502025066173424 39.0 38.0 39.0 34.0 40.0 11 36.374398061039 38.0 35.0 39.0 31.0 40.0 12 34.556749689064645 38.0 33.0 39.0 23.0 40.0 13 34.13387760308703 38.0 33.0 39.0 22.0 40.0 14 34.20247472653634 38.0 33.0 39.0 22.0 40.0 15 34.11506202761744 38.0 33.0 39.0 22.0 40.0 16 33.49823006027362 38.0 31.0 39.0 20.0 40.0 17 33.47928692158051 38.0 31.0 39.0 20.0 40.0 18 32.92636412922155 37.0 30.0 39.0 18.0 40.0 19 33.746946455336925 37.0 31.0 39.0 23.0 40.0 20 33.98048282680103 37.0 32.0 39.0 23.0 40.0 21 34.525273463660426 38.0 33.0 39.0 25.0 40.0 22 34.08872022196001 38.0 33.0 39.0 23.0 40.0 23 34.7372516503492 38.0 33.0 39.0 26.0 40.0 24 34.23296233695825 38.0 33.0 39.0 23.0 40.0 25 34.53930541824792 38.0 33.0 39.0 25.0 40.0 26 35.09771342921836 38.0 35.0 39.0 27.0 40.0 27 34.94683802659693 38.0 35.0 39.0 27.0 40.0 28 34.943935963261794 38.0 35.0 39.0 27.0 40.0 29 34.67512836049367 38.0 34.0 39.0 25.0 40.0 30 34.61762923749083 38.0 34.0 39.0 25.0 40.0 31 35.08451063558376 38.0 35.0 39.0 27.0 40.0 32 34.697420033804256 38.0 35.0 39.0 26.0 40.0 33 34.790700641005195 38.0 35.0 39.0 27.0 40.0 34 34.8358899129381 38.0 35.0 39.0 27.0 40.0 35 34.63182064610773 38.0 35.0 39.0 26.0 40.0 36 34.03313454731001 38.0 33.0 39.0 26.0 40.0 37 33.78993526166406 37.0 33.0 39.0 25.0 40.0 38 33.455496380393534 36.0 32.0 39.0 24.0 40.0 39 32.93465573875052 36.0 32.0 39.0 19.0 40.0 40 29.946678572567528 35.0 26.0 38.0 8.0 39.0 41 26.527633383295594 32.0 15.0 38.0 5.0 39.0 42 30.572567528781452 35.0 29.0 38.0 7.0 39.0 43 29.30624740887202 34.0 26.0 38.0 4.0 39.0 44 29.228178716076155 34.0 26.0 38.0 4.0 39.0 45 30.16226042032082 35.0 28.0 38.0 4.0 39.0 46 29.932550945562394 35.0 27.0 38.0 4.0 40.0 47 29.440412029211977 35.0 27.0 38.0 4.0 40.0 48 29.41171030391938 35.0 26.0 38.0 4.0 39.0 49 27.105335331823834 33.0 16.0 38.0 4.0 39.0 50 29.0489842778327 35.0 26.0 38.0 4.0 39.0 51 29.070383008578627 35.0 26.0 39.0 4.0 40.0 52 28.83882386707912 35.0 25.0 39.0 4.0 40.0 53 28.389482412220556 35.0 23.0 39.0 4.0 40.0 54 27.445961029435214 35.0 17.0 39.0 4.0 40.0 55 27.691073763434 35.0 18.0 39.0 4.0 40.0 56 26.719169563414866 34.0 16.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.11640932197736475 1 2 -0.23255512974912307 1 3 0.3278956015882599 1 4 -0.4784215544307031 1 5 -0.31115893034422015 1 6 -0.041563809959086484 1 7 0.09312978131418248 1 8 -0.17296362580010793 1 9 0.061129950540241396 1 10 -0.42115961562346627 1 11 -2.2376434263568825 1 12 -13.318273698934242 1 13 -8.096311538600506 1 14 -5.312424653690169 1 15 -5.367320288212234 1 16 -23.7289757278826 1 17 -5.0468767875870455 1 18 -14.533242609753138 1 19 -6.3094759206931705 1 20 -5.336920035559757 1 21 -7.484133004479062 1 22 -3.240113599369483 1 23 -3.299163449551358 1 24 -4.618074391616734 1 25 -4.230660750653687 1 26 2.2790636130194457 1 27 2.8883095135105066 1 28 1.7872569017350344 1 29 3.1075340713553743 1 30 2.815060373225201 1 31 2.460796833941707 1 32 1.2834534786558294 1 33 1.1462057243701125 1 34 0.48751230640839793 1 35 1.3313538877787963 1 36 1.0142904372608825 1 37 0.3576291425031357 1 38 0.5388816815539315 1 39 1.0344319059058051 1 40 3.742022551669667 1 41 -11.719325369056175 1 42 -11.082628962637884 1 43 -18.516225490035282 1 44 -22.04141967978734 1 45 -12.617584861352398 1 46 -16.896369200858135 1 47 -23.275954075468448 1 48 -14.968936402040177 1 49 -20.440313895295724 1 50 -13.959578440041705 1 51 -10.394752331445126 1 52 -10.408037851530636 1 53 -9.962076764581312 1 54 -9.170450588957053 1 55 -9.616992483534343 1 56 -4.773756808713461 2 1 0.6571524895281442 2 2 0.7331731444246898 2 3 0.8688992735588883 2 4 0.9805747735986685 2 5 0.6661972508063343 2 6 0.9737360676418874 2 7 1.0215263541415993 2 8 0.8325443301362441 2 9 0.9693306849343628 2 10 0.8236384259922005 2 11 0.8920995356994226 2 12 -3.0771476279428107 2 13 -1.3092858348061398 2 14 2.948285260630513 2 15 1.4931448280668391 2 16 1.8585395719950029 2 17 2.806244387443556 2 18 -2.323328289067703 2 19 2.4120663069690096 2 20 1.8595303928368168 2 21 2.468131377405882 2 22 1.769066327191105 2 23 1.6647288393103352 2 24 0.40028546150444555 2 25 1.4676256630550029 2 26 1.1854283621014474 2 27 2.2836583507198114 2 28 1.525322997206267 2 29 2.0175707910616154 2 30 1.9851950121480897 2 31 -0.33296081599709737 2 32 0.016011618190709953 2 33 0.14069776843376047 2 34 0.49669223296898934 2 35 0.05289611544097994 2 36 0.25847648377251176 2 37 1.2903096810588224 2 38 1.475846185837895 2 39 2.2657660552326107 2 40 1.884005415806751 2 41 -9.861920228297302 2 42 -11.477365804743147 2 43 -16.023569431283754 2 44 -16.853537182847315 2 45 -10.829335167349953 2 46 -12.44349282386915 2 47 -17.038499975101253 2 48 -10.23821424781741 2 49 -14.172872034830608 2 50 -10.534853837838522 2 51 -5.752157472204001 2 52 -5.545736749939451 2 53 -5.9167891268824135 2 54 -5.086607259703687 2 55 -4.944409864195297 2 56 -1.5203908356412477 3 1 1.0725858933336383 3 2 0.9181625736001635 3 3 1.5623453623538097 3 4 1.10531050298556 3 5 0.9077405911868865 3 6 1.271833319227511 3 7 0.9364312167208766 3 8 1.069859340707069 3 9 1.1807471754206205 3 10 0.7522853604530937 3 11 2.6917824109636967 3 12 3.5417741479557066 3 13 3.4084731503947054 3 14 3.3705897003768186 3 15 4.4735888026968595 3 16 4.528201305610224 3 17 5.326328954039745 3 18 4.935656911777965 3 19 4.863969055383386 3 20 3.8161900122871373 3 21 4.1462497706405586 3 22 3.6570155872333885 3 23 4.103946598295536 3 24 3.4034567088617393 3 25 3.9427842254228693 3 26 3.6510731823974325 3 27 3.547400422601413 3 28 3.780079868242211 3 29 2.9747589517381527 3 30 3.6009455406893167 3 31 2.502662862649842 3 32 2.0334534786558294 3 33 2.357928212408389 3 34 1.9073687657385463 3 35 1.7547988638553562 3 36 2.4006540736245157 3 37 3.233227228627534 3 38 3.3558673274869406 3 39 3.982996499207239 3 40 7.094893365066792 3 41 8.00435884147014 3 42 4.590816013438673 3 43 -0.07483793022667129 3 44 1.61527888480596 3 45 4.529544325250473 3 46 2.4074585503380383 3 47 -1.479303357765101 3 48 2.4880013970028863 3 49 5.3096861047042765 3 50 3.13850768436021 3 51 3.8588361853012856 3 52 3.680478894880846 3 53 4.168306010538306 3 54 5.864238406258263 3 55 4.726308951872358 3 56 6.39609965061668 4 1 0.5725858933336383 4 2 0.30452620996379665 4 3 1.1077999078083565 4 4 -0.3946894970144399 4 5 0.40774059118688655 4 6 0.9081969555911513 4 7 0.6637039439936032 4 8 0.3198593407070689 4 9 1.1125653572388075 4 10 0.9113762695440002 4 11 2.1236005927818766 4 12 3.8599559661375267 4 13 2.999382241303799 4 14 3.8024078821949985 4 15 3.3372251663332193 4 16 4.800928578337498 4 17 5.371783499494292 4 18 5.026566002687051 4 19 4.386696328110659 4 20 4.0207354668325905 4 21 1.8962497706405586 4 22 3.7933792235970216 4 23 2.8766738710228026 4 24 3.198911254316279 4 25 1.8291478617865025 4 26 1.560164091488339 4 27 1.797400422601413 4 28 1.8709889591512976 4 29 2.815668042647239 4 30 2.5327637225074966 4 31 2.3435719535589286 4 32 2.6016352968376495 4 33 1.6533827578629356 4 34 1.952823311193086 4 35 1.7093443184008095 4 36 1.5142904372608825 4 37 2.3695908649911743 4 38 2.742230963850581 4 39 3.232996499207239 4 40 6.344893365066792 4 41 10.617995205106507 4 42 5.658997831620486 4 43 7.425162069773329 4 44 7.251642521169597 4 45 4.847726143432286 4 46 6.157458550338035 4 47 5.270696642234899 4 48 5.988001397002886 4 49 8.877867922886097 4 50 6.615780411632937 4 51 5.449745276210379 4 52 4.748660713062666 4 53 3.9864878287201257 4 54 4.409692951712806 4 55 3.294490770054175 4 56 2.9188269233439534 5 1 -0.113777743029992 5 2 -0.8772919718543832 5 3 0.7805271805356284 5 4 0.043946866621929814 5 5 -0.20589577244948032 5 6 0.3150151374093326 5 7 -0.09765969237002992 5 8 0.37440479525252357 5 9 0.27165626632971396 5 10 -1.215896457728725 5 11 1.9781460473273285 5 12 2.6054105115920763 5 13 2.6221095140310737 5 14 3.1796806094677237 5 15 3.707679711787769 5 16 3.4236558510647725 5 17 4.064965317676112 5 18 4.9220205481415995 5 19 3.895787237201567 5 20 3.677553648650772 5 21 3.605340679731462 5 22 2.238833769051567 5 23 3.015310234659168 5 24 3.189820345225371 5 25 2.3496024072410506 5 26 1.7488004551247087 5 27 1.8383095135105094 5 28 1.6096253227876645 5 29 1.3588498608290607 5 30 1.978218267962042 5 31 -0.21551895553197653 5 32 -0.5915465213441706 5 33 -1.026162696682519 5 34 -0.2835403251705486 5 35 -1.4633829543264625 5 36 -0.0857095627391189 5 37 0.5536817740820794 5 38 0.021776418396036945 5 39 0.8989055901163354 5 40 4.4380751832486105 5 41 5.579358841470139 5 42 2.047634195256851 5 43 2.995616615227874 5 44 2.27436979389687 5 45 2.211362507068653 5 46 2.523367641247127 5 47 1.3661511876894465 5 48 2.040274124275612 5 49 3.459686104704275 5 50 3.118053138905662 5 51 1.2065634580285582 5 52 1.8327516221535731 5 53 2.0023969196292164 5 54 2.4256020426218967 5 55 1.2922180427814496 5 56 3.105190559707591 6 1 -0.30187298112523564 6 2 -1.317768162330573 6 3 -0.45280615279770586 6 4 0.06418496186002187 6 5 -0.2535148200685313 6 6 -0.0766515292573331 6 7 -0.4369454066557452 6 8 -0.6065475856998574 6 9 -0.13310563843219114 6 10 -0.43494407677634683 6 11 1.1733841425654248 6 12 3.774458130639694 6 13 2.914966656888218 6 14 3.172537752324871 6 15 3.7993463784544304 6 16 3.061751089160012 6 17 3.737584365295163 6 18 3.929163405284452 6 19 3.46245390386823 6 20 2.571601267698391 6 21 2.7815311559219396 6 22 2.5531194833372837 6 23 2.0855483298972644 6 24 2.2302965357015623 6 25 2.0412690739077135 6 26 1.7452290265532753 6 27 1.2930714182724117 6 28 2.2822443704067155 6 29 2.0862308132100083 6 30 2.054408744152518 6 31 0.934481044468022 6 32 0.9025010977034498 6 33 1.2464563509365334 6 34 0.875983484353263 6 35 1.531855140911631 6 36 1.3476237705942182 6 37 1.7310627264630298 6 38 1.0539192755388882 6 39 2.6096198758306173 6 40 5.377360897534327 6 41 0.5638826509939499 6 42 2.7607294333520933 6 43 4.601568996180255 6 44 4.250560270087345 6 45 1.7946958404019853 6 46 0.9031295460090334 6 47 2.304246425784683 6 48 2.3343217433232333 6 49 3.8692099142280867 6 50 2.2121007579532836 6 51 1.3458491723142743 6 52 1.883942098344047 6 53 1.541682633914931 6 54 1.3696496616695164 6 55 1.7815037570671635 6 56 2.5432857978028274 7 1 0.6804083034816344 7 2 0.4308475630293387 7 3 1.3107597386751664 7 4 0.7712724480172781 7 5 0.5731739949923806 7 6 1.0202476955488677 7 7 1.0447821680950895 7 8 0.46045130688042946 7 9 0.8763074291204092 7 10 1.451545402736393 7 11 2.380471628722681 7 12 4.178666325545564 7 13 4.202342072170609 7 14 4.087820144351447 7 15 4.586168083880793 7 16 4.858539571995003 7 17 5.643453689769139 7 18 4.885974036513691 7 19 4.64462444650389 7 20 4.324646671906585 7 21 3.3285964936849552 7 22 3.7225546992841245 7 23 3.22286837419405 7 24 3.6560994149928163 7 25 2.0025093839852346 7 26 2.2086841760549376 7 27 2.95807695537097 7 28 1.9904392762760352 7 29 3.087338232922086 7 30 2.7061252447062287 7 31 2.108899649119188 7 32 2.9229883623767563 7 33 1.3732559079686482 7 34 2.868785256224804 7 35 2.215686813115397 7 36 1.6305695070283264 7 37 0.7089143322216174 7 38 1.6618926974658024 7 39 2.3820451250000545 7 40 5.62819146231838 7 41 0.6729634926329311 7 42 2.336587683628945 7 43 5.09270963848369 7 44 3.030183747385241 7 45 3.3101997163709775 7 46 2.9053443854331746 7 47 3.2638256062940982 7 48 3.738529938229103 7 49 4.920151220983346 7 50 3.418634534254501 7 51 2.5501681091913504 7 52 3.2449609244791553 7 53 2.943675989396656 7 54 4.145950879831197 7 55 3.846287810223309 7 56 4.898213815521544 8 1 1.0678012043384228 8 2 0.8200764491982468 8 3 1.5121061279040475 8 4 1.2321047613587695 8 5 1.136209490708417 8 6 1.195278295304071 8 7 0.8826034655247099 8 8 0.9586153215683098 8 9 1.4032352136981316 8 10 1.1577877527975886 8 11 2.2623565736431175 8 12 4.971199985276286 8 13 4.351056882452127 8 14 4.3454700831519375 8 15 4.606363922314081 8 16 5.6394453247489835 8 17 5.505754791360324 8 18 6.493073179720543 8 19 4.637892500359463 8 20 3.9525536486507704 8 21 3.9895512060472527 8 22 3.970412716419986 8 23 3.5692576030802243 8 24 4.460872976804318 8 25 3.7693392493463094 8 26 3.4106425603878634 8 27 3.3883095135105066 8 28 2.2872569017350344 8 29 4.028586702934319 8 30 3.0255866890146734 8 31 3.381849465520652 8 32 2.941348215497932 8 33 1.8041004612122222 8 34 2.90856493798735 8 35 2.568195993041954 8 36 3.30376412147141 8 37 2.8839449319768207 8 38 3.328355365764452 8 39 3.7712740111689627 8 40 5.136759393774927 8 41 -0.2193253690561754 8 42 4.943686826835798 8 43 6.1943008257541905 8 44 6.142790846528452 8 45 4.171888822858126 8 46 5.472051851773443 8 47 5.039835398215761 8 48 5.767905703222983 8 49 7.217580841546379 8 50 4.829895244168821 8 51 5.263142405396977 8 52 5.223541095837785 8 53 6.143186393313428 8 54 7.0137599373587385 8 55 6.540902253307763 8 56 6.278874770233905 9 1 0.8835906780226352 9 2 0.8463922386719318 9 3 1.301579812114575 9 4 0.942631077148242 9 5 0.7677884380768347 9 6 1.195278295304071 9 7 0.9615508339457577 9 8 1.1165100584104195 9 9 1.1137615294876113 9 10 0.8156824896396913 9 11 2.551830257853645 9 12 3.5764631431710185 9 13 4.245793724557387 9 14 4.634943767362465 9 15 4.501100764419348 9 16 5.007866377380559 9 17 5.216281107149797 9 18 6.177283706036338 9 19 4.295787237201566 9 20 4.715711543387613 9 21 4.305340679731465 9 22 4.180939032209466 9 23 3.5429418136065394 9 24 4.72403087154116 9 25 4.2430234598726315 9 26 2.9632741393352333 9 27 3.572520039826294 9 28 3.6819937438402945 9 29 3.765428808197477 9 30 3.657165636383098 9 31 2.1713231497311796 9 32 3.072927162866357 9 33 3.409363619106955 9 34 3.040143885355768 9 35 2.936617045673536 9 36 4.093237805681937 9 37 3.0155238793452384 9 38 4.302039576290774 9 39 4.8765371690637025 9 40 7.031496235880194 9 41 4.491200946733297 9 42 5.785792089993695 9 43 7.773248194175245 9 44 6.7217382149495 9 45 5.908730928121283 9 46 6.8931044833523885 9 47 7.671414345584186 9 48 7.00474780848614 9 49 9.822843999441119 9 50 8.250947875747766 9 51 4.973668721186456 9 52 5.170909516890415 9 53 4.1431863933134245 9 54 3.934812568937687 9 55 4.014586463834078 9 56 4.278874770233905 10 1 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0.3523718836961365 118 27 2.209738084939076 118 28 0.16319675135909506 118 29 0.5981355751147746 118 30 1.8282182679620433 118 31 0.07733818732516795 118 32 -6.323689378487028 118 33 0.377408731888913 118 34 -0.5406831823134013 118 35 -0.4919543828978945 118 36 0.12143329440373662 118 37 1.7786817740820808 118 38 0.2324907041103188 118 39 1.395334161544902 118 40 3.091646611820039 118 41 1.1829302700415667 118 42 -2.763080090457432 118 43 -8.207954813343552 118 44 -6.0827730632459875 118 45 -2.8957803500742045 118 46 -9.323060930181445 118 47 -7.219563098024839 118 48 -10.784725875724387 118 49 -8.583171038152866 118 50 -9.156946861094337 118 51 -7.618436541971441 118 52 -6.627962663560712 118 53 -6.279745937513642 118 54 -8.856540814520962 118 55 -7.182781957218552 118 56 -4.409095154578125 119 1 -0.23619153613343968 119 2 -0.5410850753026608 119 3 -0.34361075049885415 119 4 -0.15174278855048584 119 5 0.018242158585003665 119 6 -0.5996400350044624 119 7 -0.4123148647838235 119 8 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28 0.7346253227876645 120 29 0.46718319416239495 120 30 0.8401230298668025 120 31 -4.0417094317224525 120 32 -11.978451283248933 120 33 -2.5868769823968023 120 34 -3.290683182313405 120 35 -3.634811525755037 120 36 -2.5809476579772124 120 37 -2.649889654489346 120 38 -3.517509295889681 120 39 -3.3665706003598572 120 40 -2.6940676738942457 120 41 -1.7932602061489078 120 42 -4.62022294760029 120 43 -12.041288146676887 120 44 -10.082773063245988 120 45 -8.943399397693252 120 46 -13.715918073038585 120 47 -13.767182145643888 120 48 -8.713297304295814 120 49 -17.130790085771913 120 50 -9.049804003951479 120 51 -6.582722256257156 120 52 -7.116057901655953 120 53 -6.791650699418405 120 54 -8.96368367166382 120 55 -6.956591481028077 120 56 -3.099571345054315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 37.0 5 10.0 6 16.0 7 14.0 8 54.0 9 89.0 10 78.0 11 66.0 12 51.0 13 52.0 14 58.0 15 77.0 16 94.0 17 124.0 18 159.0 19 215.0 20 247.0 21 262.0 22 372.0 23 428.0 24 519.0 25 652.0 26 650.0 27 748.0 28 852.0 29 1055.0 30 1210.0 31 1435.0 32 1676.0 33 1860.0 34 2284.0 35 2578.0 36 3135.0 37 4136.0 38 3793.0 39 2268.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 98.23324935421118 1.176770736996524 0.24555920528111747 0.3444207035111777 8 95.47150556494563 2.5098064228083046 1.8273431769620818 0.19134483528398763 9 89.36760531938643 5.804126670280958 1.7061581146155562 3.1221098957170645 10 37.43661702331218 41.13913958605734 11.777274611729439 9.646968778901043 11 24.73769812163153 33.59696399528016 22.231080779411297 19.434257103677012 12 31.364607583633635 17.798258761998916 23.65022164110087 27.18691201326657 13 27.019009951518246 21.15973462618015 24.872416432763462 26.94883898953815 14 22.142492664880724 22.939788238295698 31.123230003827018 23.794489092996557 15 28.01892220162373 20.6546058940101 27.817554177587418 23.50891772677875 16 28.266487986981907 21.782967997192177 24.53974027631537 25.410803739510545 17 24.10944924578244 27.52495455560162 20.068884140702238 28.2967120579137 18 27.97780399910706 25.729502184520204 18.646554198424596 27.646139617948144 19 31.326416600159618 29.55147645650439 19.01675977653631 20.10534716679968 20 29.92148602068173 30.1704327843738 18.6710072769054 21.237073918039066 21 26.797844181522468 27.40058041266703 23.006027362311446 22.79554804349906 22 24.300794081066428 28.577351149663556 22.483018145868545 24.638836623401474 23 28.35730458908697 30.962783429537268 20.21877092834136 20.46114105303441 24 23.360015307586824 34.056191599961736 22.601014127627007 19.98277896482444 25 26.059639610907354 31.184819008132674 22.656673576782012 20.09886780417796 26 21.976269456494006 32.275452921663685 25.37956111252871 20.3687165093136 27 21.90831896826917 27.845878822703185 29.196194854114793 21.04960735491285 28 22.5024743782127 32.15095303470515 27.40014686631972 17.946425720762427 29 23.736004338256404 29.560751539124052 29.280040830648506 17.423203291971035 30 18.137161084529506 31.084529505582136 32.49760765550239 18.280701754385966 31 18.64552762536685 30.387903534515758 34.62421845093786 16.342350389179533 32 19.09323116219668 29.964878671775224 34.195402298850574 16.746487867177525 33 17.39324837916387 30.465331672575136 36.370604579860114 15.770815368400882 34 14.663645647178964 33.472683175899924 38.74457492979321 13.119096247127903 35 16.199227848505153 32.81324782234134 38.70010529338566 12.287419035767844 36 16.59062629614268 31.873145518935647 38.305203713747886 13.231024471173786 37 16.372232693352075 31.076893588473947 39.424336325591796 13.126537392582179 38 16.407649015093373 31.389102072141213 38.72473778459965 13.47851112816577 39 15.412392547854154 31.371233183140003 37.775221295497396 15.441152973508451 40 12.649654951051195 24.36527042208313 34.45995827315038 28.525116353715298 41 11.07968242743724 22.178650638037993 32.715116839703 34.026550094821765 42 14.823687236489077 31.838507729653763 39.47553340999106 13.862271623866103 43 11.88618925831202 27.266624040920718 40.77365728900256 20.073529411764707 44 12.645041348804412 27.89601897557536 38.77171613564972 20.68722353997051 45 13.134604939454936 33.68797725166938 40.371896865714554 12.805520943161122 46 13.807865563400531 32.35679371266094 40.37570684642663 13.459633877511902 47 13.868240548731855 31.963630563088213 40.46897431807306 13.699154570106876 48 13.105695211933433 33.452582489539076 40.677803686076594 12.763918612450889 49 11.240819729963759 26.25958115519066 36.573554408133155 25.92604470671242 50 13.792552851759595 32.70741521364246 41.63952225841477 11.860509676183177 51 15.24957631183449 33.87586736162185 38.49646660058197 12.378089725961692 52 13.807745107429046 34.8497908884845 38.917089678511 12.425374325575456 53 13.506949992011503 35.01198274484742 39.693241731906056 11.787825531235022 54 11.815101336231699 32.382839971868805 38.00907870340771 17.792979988491783 55 13.649149725099091 34.66947960618847 38.97199846566935 12.70937220304309 56 15.768284500111832 34.66466434482538 37.773588522861616 11.79346263220117 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 9.0 7 18.0 8 37.0 9 56.0 10 50.0 11 44.0 12 95.5 13 212.0 14 277.0 15 311.0 16 345.0 17 326.0 18 307.0 19 308.5 20 310.0 21 372.5 22 473.0 23 511.0 24 649.5 25 788.0 26 952.0 27 1116.0 28 1291.5 29 1558.5 30 1650.0 31 1712.5 32 1775.0 33 1744.5 34 1714.0 35 1553.0 36 1392.0 37 1308.5 38 1093.5 39 962.0 40 888.0 41 814.0 42 759.5 43 705.0 44 674.5 45 644.0 46 714.0 47 842.5 48 901.0 49 928.5 50 956.0 51 974.0 52 992.0 53 1002.0 54 1111.5 55 1211.0 56 1165.5 57 1120.0 58 1050.0 59 980.0 60 1084.0 61 1188.0 62 1206.0 63 1181.5 64 1139.0 65 1068.0 66 997.0 67 864.0 68 731.0 69 620.5 70 510.0 71 405.5 72 251.0 73 201.0 74 193.0 75 185.0 76 151.5 77 118.0 78 88.0 79 44.0 80 30.0 81 19.0 82 8.0 83 6.0 84 4.0 85 3.5 86 3.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.015945402940332302 14 0.0031890805880664605 15 0.2264247217527187 16 0.05102528940906337 17 0.0 18 0.0 19 0.10205057881812674 20 0.07972701470166152 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006378161176132921 26 0.015945402940332302 27 0.09886149823006027 28 0.11480690117039258 29 0.025512644704531684 30 0.022323564116465223 31 0.028701725292598144 32 0.11799598175845903 33 0.14669770705105717 34 0.06697069234939568 35 0.05102528940906337 36 0.04464712823293045 37 0.17221035175558885 38 0.27107184998564915 39 0.20410115763625347 40 0.644194278789425 41 0.7845138246643492 42 0.15626494881525657 43 0.24555920528111747 44 0.5070638135025671 45 0.1849666741078547 46 0.1785885129317218 47 0.038268967056797526 48 0.15945402940332304 49 0.561278183499697 50 0.1371304652868578 51 0.2678827693975827 52 0.10842873999425966 53 0.19772299646012054 54 0.23918104410498453 55 0.23280288292885162 56 0.19134483528398763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 31357.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.78304684759384 #Duplication Level Percentage of deduplicated Percentage of total 1 88.657707253886 69.84724303983162 2 6.691224093264249 10.543100424147719 3 2.076586787564767 4.907995025034283 4 0.939119170984456 2.959466785725675 5 0.5181347150259068 2.0410115763625347 6 0.3400259067357513 1.6072966163854963 7 0.15786917098445596 0.8706190005421437 8 0.14167746113989638 0.892942564658609 9 0.08500647668393782 0.602736231144561 >10 0.37645725388601037 4.818700768568421 >50 0.016191709844559588 0.9088879675989413 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 96 0.3061517364543802 No Hit GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.22004656057658575 No Hit GTTGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCG 64 0.20410115763625347 Illumina Single End Adapter 2 (97% over 34bp) GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 56 0.1785885129317218 No Hit GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.14669770705105717 No Hit GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 45 0.1435086264629907 No Hit GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 38 0.12118506234652549 No Hit GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 34 0.10842873999425966 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 34 0.10842873999425966 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.006378161176132921 0.0 0.0 0.0 0.0 12 0.012756322352265842 0.0 0.0 0.0 0.0 13 0.022323564116465223 0.0 0.0 0.0 0.0 14 0.025512644704531684 0.0 0.0 0.0 0.0 15 0.041458047644863986 0.0 0.0 0.0 0.0 16 0.05102528940906337 0.0 0.0 0.0 0.0 17 0.07015977293746213 0.0 0.0 0.0 0.0 18 0.08291609528972797 0.0 0.0 0.0 0.0 19 0.08610517587779443 0.0 0.0 0.0 0.0 20 0.09567241764199381 0.0 0.0 0.0 0.0 21 0.09886149823006027 0.0 0.0 0.0 0.0 22 0.1211850623465255 0.0 0.0 0.0 0.0 23 0.1371304652868578 0.0 0.0 0.0 0.0 24 0.17221035175558885 0.0 0.0 0.0 0.0 25 0.1785885129317218 0.0 0.0 0.0 0.0 26 0.2168574799885193 0.0 0.0 0.0 0.0 27 0.22961380234078516 0.0 0.0 0.0 0.0 28 0.24555920528111747 0.0 0.0 0.0 0.0 29 0.2615046082214498 0.0 0.0 0.0 0.0 30 0.2742609305737156 0.0 0.0 0.0 0.0 31 0.2902063335140479 0.0 0.0 0.0 0.0 32 0.30296265586631377 0.0 0.0 0.0 0.0 33 0.3061517364543802 0.0 0.0 0.0 0.0 34 0.3061517364543802 0.0 0.0 0.0 0.0 35 0.318908058806646 0.0 0.0 0.0 0.0 36 0.3316643811589119 0.0 0.0 0.0 0.0 37 0.3316643811589119 0.0 0.0 0.0 0.0 38 0.33485346174697833 0.0 0.0 0.0 0.0 39 0.34123162292311127 0.0 0.0 0.0 0.0 40 0.36036610645151 0.0 0.0 0.0 0.0 41 0.36674426762764295 0.0 0.0 0.0 0.0 42 0.3699333482157094 0.0 0.0 0.0 0.0 43 0.3763115093918423 0.0 0.0 0.0 0.0 44 0.3954459929202411 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACCTG 15 0.0081420075 50.326893 41 CGTTGTA 15 0.0081420075 50.326893 38 AAACCAC 20 3.9904309E-4 50.32689 40 AACCACC 20 3.9904309E-4 50.32689 41 CGCCTGG 15 0.008246398 50.16533 46 ACCACAA 15 0.008246398 50.16533 45 CCGTTGT 20 4.0544514E-4 50.16533 37 CAACAAC 15 0.008246398 50.16533 43 AAGATTA 20 4.0544514E-4 50.16533 48 CACCACA 15 0.008246398 50.16533 44 ATGTCAG 15 0.008246398 50.16533 44 AGATTAA 20 4.0544514E-4 50.16533 49 AAAGATT 15 0.008246398 50.16533 47 CCTGGTC 15 0.008246398 50.16533 48 TGGTTGA 45 1.2551027E-10 50.084934 14 GGTTGAT 50 5.456968E-12 50.084934 15 CATTGGC 15 0.008298963 50.084934 12 GACAGTG 20 4.0867663E-4 50.084934 9 GTACCTG 45 1.2551027E-10 50.084934 9 GATACAA 15 0.008298963 50.084934 9 >>END_MODULE