FastQCFastQC Report
Fri 17 Jun 2016
SRR1526265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76749
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG16452.1433504019596348No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG4330.5641767319443902No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG4080.5316030176288942No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG4040.5263912233384149No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1710.22280420591799244No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1490.19413933732035596No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG1260.1641715201500997No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1190.1550508801417608No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1190.1550508801417608No Hit
CACAAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC890.11596242296316565No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC860.11205357724530614No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG770.10032704009172759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCG150.00817205350.350536
AAGCCGC150.00819341550.31728735
TAGAGCG150.00821481850.28411534
GCAAGCG150.00821481850.28411549
CGATTCA402.4974725E-950.28411549
ATATGCC700.050.28411534
ATTCCTT150.00821481850.28411547
ACGATTC402.4974725E-950.28411548
ATTGACC150.00821481850.28411534
CTGTGAT150.00821481850.28411534
CCCTGAT150.00823626450.25098845
TCCAAGA150.00823626450.25098845
GATTCAG451.2914825E-1050.15187550
ACGGGTA150.00834411450.08601833
TATTGAC150.00834411450.08601833
CTATTGA150.00840932649.98755332
TCGAGCG150.00840932649.98755332
GGCTATA150.00843114849.95481528
AATTGAC204.1778013E-449.9548128
GAATAGC301.0461481E-649.92212313