##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526265_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 76749 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.93719787879972 39.0 38.0 40.0 35.0 40.0 2 37.11751293176458 39.0 38.0 39.0 33.0 40.0 3 37.18130529388005 39.0 38.0 40.0 33.0 40.0 4 36.732726159298494 39.0 38.0 39.0 33.0 40.0 5 36.35150946592138 39.0 38.0 39.0 31.0 40.0 6 36.41913249684035 39.0 36.0 39.0 30.0 40.0 7 37.32988051961589 39.0 38.0 39.0 33.0 40.0 8 37.19112952611761 39.0 38.0 39.0 33.0 40.0 9 37.216094020769 39.0 38.0 39.0 33.0 40.0 10 36.56299104874331 39.0 36.0 39.0 31.0 40.0 11 35.97368043883308 38.0 35.0 39.0 29.0 40.0 12 34.09274387939908 38.0 33.0 39.0 22.0 40.0 13 34.08798811710902 38.0 33.0 39.0 22.0 40.0 14 34.12827528697442 38.0 33.0 39.0 22.0 40.0 15 34.13034697520489 38.0 33.0 39.0 22.0 40.0 16 33.527277228367794 38.0 31.0 39.0 21.0 40.0 17 33.50090554925797 37.0 31.0 39.0 22.0 40.0 18 33.06474351457348 36.0 30.0 39.0 19.0 40.0 19 33.874890878057045 37.0 31.0 39.0 23.0 40.0 20 33.83396526339106 37.0 31.0 39.0 23.0 40.0 21 34.752153122516255 38.0 33.0 39.0 26.0 40.0 22 34.445334792635734 38.0 33.0 39.0 24.0 40.0 23 34.922722120157914 38.0 33.0 39.0 26.0 40.0 24 34.458064600190234 38.0 33.0 39.0 24.0 40.0 25 34.697728960637924 38.0 33.0 39.0 26.0 40.0 26 35.349528984090995 38.0 35.0 39.0 28.0 40.0 27 35.23751449530287 38.0 35.0 39.0 28.0 40.0 28 35.090646132197165 38.0 34.0 39.0 27.0 40.0 29 34.94246179103311 38.0 34.0 39.0 27.0 40.0 30 34.61151285358767 38.0 33.0 39.0 26.0 40.0 31 34.94751723149487 38.0 34.0 39.0 28.0 40.0 32 34.564958501087965 38.0 34.0 39.0 26.0 40.0 33 34.77866812596907 38.0 34.0 39.0 27.0 40.0 34 34.58726498065121 38.0 33.0 39.0 27.0 40.0 35 34.439706054802016 38.0 33.0 39.0 27.0 40.0 36 33.649089890422026 36.0 32.0 38.0 26.0 40.0 37 33.36401777221853 36.0 32.0 38.0 25.0 40.0 38 33.094333476657674 35.0 32.0 38.0 24.0 40.0 39 32.72656321255 35.0 31.0 38.0 23.0 40.0 40 29.29595173878487 35.0 23.0 38.0 8.0 39.0 41 26.005068469947492 31.0 14.0 37.0 4.0 39.0 42 29.96555003973993 34.0 28.0 38.0 4.0 39.0 43 28.387040873496723 33.0 24.0 37.0 4.0 39.0 44 28.514599538756205 33.0 25.0 37.0 4.0 39.0 45 29.56729077903295 33.0 28.0 38.0 4.0 39.0 46 29.139337320355967 34.0 26.0 38.0 4.0 39.0 47 28.738328838160758 34.0 26.0 38.0 4.0 39.0 48 28.744908728452486 33.0 26.0 38.0 4.0 39.0 49 26.357737560098503 33.0 14.0 37.0 4.0 39.0 50 28.361711553244994 33.0 25.0 38.0 4.0 39.0 51 28.57496514612568 34.0 26.0 38.0 4.0 40.0 52 28.266426924129306 34.0 24.0 38.0 4.0 40.0 53 27.877040743201867 34.0 23.0 38.0 4.0 40.0 54 26.95629910487433 33.0 16.0 38.0 4.0 39.0 55 27.210856167507067 33.0 18.0 38.0 4.0 39.0 56 26.275508475680464 33.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.8690940792045581 1 2 1.8176661440349875 1 3 1.723133076243478 1 4 2.4918463286557895 1 5 2.883820335308009 1 6 2.3501770646423594 1 7 1.4079104138216252 1 8 1.5094313945349214 1 9 1.6346320499305094 1 10 2.065089600798366 1 11 -4.0399148128335085 1 12 -14.756766861810096 1 13 -8.339307272579 1 14 -7.02539381537429 1 15 -6.522793664676055 1 16 -23.401598810740296 1 17 -6.624379565176522 1 18 -14.796989686275772 1 19 -7.842085594748035 1 20 -6.5496923745082825 1 21 -5.8496066756024625 1 22 -3.333429868201925 1 23 -3.074420043676799 1 24 -4.000498421448512 1 25 -3.481926678640747 1 26 2.120751706635886 1 27 2.5120776737926676 1 28 2.5283218938272682 1 29 2.6112481863431043 1 30 3.031909983084553 1 31 2.070705996639738 1 32 2.9016956660959465 1 33 2.5354594370262475 1 34 2.3843897376024614 1 35 2.772421224141425 1 36 2.1897503935977127 1 37 2.496761910512994 1 38 1.7805628836728076 1 39 2.504759102518907 1 40 4.987823625157393 1 41 -12.571419567052985 1 42 -13.120289841713126 1 43 -19.24257341809304 1 44 -21.864760467809365 1 45 -16.302647082411937 1 46 -19.733524425043605 1 47 -22.925781097781062 1 48 -17.774890710064817 1 49 -20.277983282902944 1 50 -17.220995535248157 1 51 -14.904648938785972 1 52 -14.57891571355698 1 53 -13.970868814435612 1 54 -13.381873361761018 1 55 -13.652440994919095 1 56 -10.161026409167517 2 1 1.1831854099951968 2 2 2.126912613471127 2 3 2.138348002539736 2 4 2.7070818713833447 2 5 3.017620974417376 2 6 2.0093874553237328 2 7 1.5967569244906272 2 8 1.8044525263769984 2 9 1.5429920128209318 2 10 1.7855307924280979 2 11 0.6787739821360788 2 12 -2.3786615118461754 2 13 -1.6293804609100988 2 14 2.9444031127774437 2 15 2.410615262238693 2 16 2.5987104357291386 2 17 3.6102354229690263 2 18 -2.5074319087270673 2 19 2.3082081893979307 2 20 2.8825311076069653 2 21 2.606634948972328 2 22 3.1164206626711817 2 23 2.585718086412882 2 24 1.9297149586153992 2 25 2.6250725997841613 2 26 2.3748492223559126 2 27 2.5208396570933544 2 28 1.7136636111759955 2 29 2.2912811726331768 2 30 2.5383526178644686 2 31 2.370881236305756 2 32 2.798510427460755 2 33 1.7363665600032547 2 34 2.5776687810000496 2 35 2.077441325161942 2 36 2.501264670476381 2 37 1.9926901653320854 2 38 3.2308257431718275 2 39 2.960588398467781 2 40 6.091730438887939 2 41 -7.1765118255830345 2 42 -7.056997397635197 2 43 -13.543676397167363 2 44 -15.307910658511355 2 45 -7.279144350734793 2 46 -12.276045814591072 2 47 -18.034017362083713 2 48 -9.938379010240055 2 49 -13.976571057359187 2 50 -8.391596504220427 2 51 -4.437274441311484 2 52 -4.031961791280924 2 53 -3.822018180480349 2 54 -2.732868104571036 2 55 -3.481633861633867 2 56 0.15553889337861193 3 1 1.2507116518333632 3 2 2.079635979038528 3 3 2.1518201903238463 3 4 2.695510667151595 3 5 3.089610395030654 3 6 2.580261081243229 3 7 1.6947815546253509 3 8 1.8028821486598332 3 9 1.7978064602959307 3 10 1.725277878964171 3 11 2.5219841647958035 3 12 4.487691079277056 3 13 2.956370427593086 3 14 3.488828271881502 3 15 3.5980687707931196 3 16 4.250086582518129 3 17 4.877695543640158 3 18 5.415784886049359 3 19 4.057195709027653 3 20 4.442412089506291 3 21 3.324297565717508 3 22 3.7957330025339786 3 23 2.966576008555208 3 24 3.179074409809708 3 25 3.595813983369581 3 26 2.543871455598328 3 27 2.309830482781429 3 28 2.9069027218462935 3 29 2.973734267930304 3 30 2.383463784076554 3 31 1.9282826744411352 3 32 1.861408187606223 3 33 0.8777005545482623 3 34 1.6967695331283181 3 35 1.1820780720005217 3 36 3.99651221159548 3 37 3.69101234071848 3 38 3.3371981706451734 3 39 2.6656053420168355 3 40 6.8369986428717375 3 41 7.333530326660952 3 42 3.8075368613944747 3 43 -1.2443954648589077 3 44 2.7565574793512795 3 45 2.8921094718951785 3 46 1.4435177857064723 3 47 -3.1778309003926637 3 48 1.385449405331478 3 49 3.591740373337565 3 50 2.1127426973664782 3 51 2.7839835138914246 3 52 3.4767579376222884 3 53 3.8691959694493967 3 54 4.252502587221674 3 55 2.9888182418457596 3 56 4.163804039258437 4 1 1.49570664682836 4 2 2.3456519950545456 4 3 2.302721091224747 4 4 2.907472629113556 4 5 3.2167375221577785 4 6 2.4984292494114015 4 7 1.6297164895602876 4 8 1.8561854519631353 4 9 1.8240827365722012 4 10 2.0433459470322433 4 11 2.4676798604915007 4 12 4.981685073271052 4 13 4.2779419991646535 4 14 4.261851294904531 4 15 4.138359061083413 4 16 4.601938434369984 4 17 5.000568416513033 4 18 5.119989090253561 4 19 3.525163676995618 4 20 3.90037004746425 4 21 3.020243511663459 4 22 3.549737006537981 4 23 2.9270364690156683 4 24 3.815460796196099 4 25 2.686404573960168 4 26 3.0148424265693023 4 27 2.689460112411055 4 28 3.188434253377828 4 29 2.704464998661038 4 30 3.192022342635113 4 31 1.7974017935602546 4 32 2.9560027822008124 4 33 2.5341069609546665 4 34 1.8914642278230147 4 35 2.5421881821106282 4 36 4.052318017401291 4 37 4.286607936314077 4 38 3.9122732457202503 4 39 4.80199172840322 4 40 7.528189834062932 4 41 9.964160957291586 4 42 6.304283608141219 4 43 6.8812301607667195 4 44 6.195996918790716 4 45 5.466183545969251 4 46 4.4930673352560255 4 47 4.873220150658383 4 48 5.117431387313463 4 49 7.731380012977201 4 50 4.728858813482592 4 51 3.898097628005541 4 52 4.655686866551218 4 53 4.225552325805754 4 54 4.862612697331784 4 55 4.49882825185577 4 56 4.1142544897088875 5 1 1.4497597565418445 5 2 2.2672103458581745 5 3 2.3105827264448777 5 4 2.9328045648228454 5 5 3.1262413656238905 5 6 2.3815529391388637 5 7 1.584293708288449 5 8 1.9342776951497243 5 9 1.8644391307021806 5 10 2.0358337178218946 5 11 2.5901466669205746 5 12 4.3737186162479915 5 13 4.360925926488207 5 14 4.707169254373433 5 15 4.5123981945565035 5 16 5.089709224027565 5 17 4.878800422103531 5 18 5.089590767402413 5 19 4.018524962809735 5 20 4.465359565283961 5 21 3.3614384802169184 5 22 3.450330996754616 5 23 3.393144086066684 5 24 3.740862613107346 5 25 3.6615967472655484 5 26 2.0763378842911777 5 27 2.6871889733474674 5 28 1.938434253377828 5 29 2.752333622001359 5 30 2.9137204558426575 5 31 2.4859063358383793 5 32 2.7610342287416927 5 33 2.1275730685717207 5 34 2.0462510901570496 5 35 1.8659128501749223 5 36 3.8459937686242114 5 37 3.7024010879562823 5 38 3.417514335866997 5 39 4.173934425817617 5 40 7.774870476969991 5 41 9.961016303203532 5 42 6.717106808700272 5 43 7.094717232744358 5 44 6.977618162676112 5 45 6.112235258058696 5 46 4.989922681167972 5 47 5.078670884411004 5 48 4.8925886200178645 5 49 7.649619006687896 5 50 5.721870693286927 5 51 4.870843959242439 5 52 4.947266181715438 5 53 4.738480348167737 5 54 5.398601516339468 5 55 4.364306938089172 5 56 4.1991601500862465 6 1 1.3828149089366235 6 2 1.8780594024619504 6 3 1.9437467322503892 6 4 2.853341574982501 6 5 2.974572279992536 6 6 2.1928736938558444 6 7 1.3159700508138457 6 8 1.3519119476896364 6 9 1.3198092322987023 6 10 1.7011094797957753 6 11 2.4961698889815267 6 12 4.521214987800967 6 13 4.014409235631895 6 14 4.60230713536037 6 15 3.9296696023939504 6 16 4.721240428671976 6 17 4.540810581755196 6 18 5.067638662903136 6 19 4.030861682693626 6 20 3.8116948337890406 6 21 2.6388332552532034 6 22 3.2221016789026535 6 23 3.213717380696579 6 24 3.0576260383613416 6 25 3.231988619544218 6 26 2.5536600903869626 6 27 2.261753559704509 6 28 2.111511176454748 6 29 2.6385818077778467 6 30 3.018945419558193 6 31 1.941276437434901 6 32 2.202797653995688 6 33 1.6957878726355773 6 34 2.543529754888546 6 35 1.7982423131647636 6 36 2.0067339718172406 6 37 2.153417053123192 6 38 2.6825723910193915 6 39 3.002490303901798 6 40 6.079115759988856 6 41 1.5539045470351738 6 42 3.9342693631269796 6 43 5.047184576721136 6 44 4.423917146710945 6 45 3.331212035997744 6 46 4.28473400192269 6 47 4.772792800231038 6 48 3.784098053980127 6 49 6.064713346310537 6 50 3.8973061069298893 6 51 4.621388226296137 6 52 4.307752393616745 6 53 4.3288286790821076 6 54 4.907128366847452 6 55 3.349611727639246 6 56 1.8354083358627342 7 1 1.4497597565418445 7 2 2.3615499684996877 7 3 2.433224235878839 7 4 3.0177102252002044 7 5 3.4752979693974737 7 6 2.9570246372520685 7 7 2.0465578592318465 7 8 2.236164487602551 7 9 2.0531183759852 7 10 2.0452676800860488 7 11 2.703354214090382 7 12 4.864284653983837 7 13 4.31375611516745 7 14 4.282640952486638 7 15 4.993530270028202 7 16 4.919897903272847 7 17 5.06747966738655 7 18 5.834873786270336 7 19 4.386449491111627 7 20 4.946491640755653 7 21 3.512381876443328 7 22 3.6861800533583846 7 23 3.4591818219157418 7 24 4.155956952729987 7 25 3.6710307095297026 7 26 3.2178473182534404 7 27 3.319264445045583 7 28 2.9667361401702834 7 29 3.261767584265506 7 30 3.30051290867285 7 31 1.7594912414987576 7 32 3.289336115534148 7 33 2.5332334459302075 7 34 2.480213354307992 7 35 2.8187430388541657 7 36 2.7610881082468524 7 37 2.8156086351260967 7 38 3.2948728264330356 7 39 3.947519331477995 7 40 6.539021420366215 7 41 -0.05785162132476884 7 42 3.868050204926689 7 43 6.141887044065108 7 44 5.288938917393093 7 45 4.112235258058696 7 46 4.810677398149107 7 47 5.059802959882703 7 48 4.7227772992631465 7 49 7.404335987819973 7 50 4.8067763536642865 7 51 5.068957166789605 7 52 5.428398257187137 7 53 5.2950841217526445 7 54 5.662752459735696 7 55 5.54355222110804 7 56 5.868971470840965 8 1 1.3417829211224515 8 2 2.0971188084025414 8 3 2.0890247215877835 8 4 2.7183453830556132 8 5 2.9684793630085338 8 6 2.3116855750439598 8 7 1.4133615207300707 8 8 1.5737322066386028 8 9 1.5218275110496506 8 10 1.6246624120120714 8 11 2.2195133771765043 8 12 4.198956800443767 8 13 3.6966255341848253 8 14 4.085742017310103 8 15 4.626166175128148 8 16 4.091670740933971 8 17 4.216648357840498 8 18 4.533080399384481 8 19 3.312659093203905 8 20 3.9262226327227765 8 21 2.2933458218944764 8 22 2.4172654260465976 8 23 2.3569961316486 8 24 2.744412024652277 8 25 2.5358728541215214 8 26 1.166474256418951 8 27 1.735573057038856 8 28 0.992889698922383 8 29 1.7466358824160793 8 30 1.2192500654073868 8 31 1.0393343201462457 8 32 2.1457717590985013 8 33 1.3625814750727443 8 34 1.3907308211490133 8 35 0.7560679034160955 8 36 1.076612113477566 8 37 1.101056047791893 8 38 0.9810301214078194 8 39 1.9405139139551117 8 40 3.241244472860142 8 41 -0.8642218809922433 8 42 2.6276242755412973 8 43 3.99215792020933 8 44 3.7552195077162764 8 45 2.611581419089898 8 46 3.7290409326157565 8 47 3.509083737017022 8 48 3.281622806455374 8 49 6.416198494825387 8 50 2.906159876922274 8 51 2.4712549437371116 8 52 2.414854449976229 8 53 2.8423473386403693 8 54 2.6482745968550674 8 55 2.420537089406192 8 56 2.4063336976296803 9 1 1.156166557288266 9 2 1.9865758859784322 9 3 1.7926478311514913 9 4 2.427517399158326 9 5 2.5899568953771563 9 6 2.115950616932764 9 7 1.322838182681977 9 8 1.349164694942381 9 9 1.492886155375622 9 10 1.5898457435320381 9 11 2.3340819768936143 9 12 4.022588614174595 9 13 3.7534202246428805 9 14 4.110548893602129 9 15 4.462636635360987 9 16 4.579756912188458 9 17 4.8388875048321225 9 18 5.4343969046613765 9 19 4.030861682693626 9 20 4.230650877745084 9 21 2.78306402448397 9 22 2.6149588217597994 9 23 2.8881679301471337 9 24 3.038395269130568 9 25 2.330889718445313 9 26 1.332506244233123 9 27 1.5955447684957136 9 28 0.9439287588723317 9 29 1.1509444451404818 9 30 1.920044320657091 9 31 1.6363313824898427 9 32 1.910215236413272 9 33 1.404579081426789 9 34 2.116331952690743 9 35 1.313352203274654 9 36 2.152338367421642 9 37 2.3374829871891265 9 38 2.7993306327776395 9 39 3.286830963242455 9 40 5.668401474274571 9 41 4.718739711870338 9 42 4.968610022467637 9 43 6.324657104193662 9 44 6.577763300557098 9 45 5.071596651382357 9 46 5.099294441483128 9 47 5.511803789242023 9 48 5.165966185848259 9 49 7.90537268696988 9 50 4.895932480556265 9 51 3.881003610911524 9 52 4.119565580429931 9 53 4.72168582193925 9 54 4.812348147067233 9 55 4.573512826540345 9 56 4.350518225972625 10 1 1.4308918320135433 10 2 2.241695766098317 10 3 2.0565089700126293 10 4 2.8376073092482343 10 5 3.0812156366358963 10 6 2.3860555120376645 10 7 1.764396624240419 10 8 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115 18 -3.871639524662722 115 19 -3.4517515944402 115 20 -3.126266177802112 115 21 1.4300608632616303 115 22 2.0990774428647185 115 23 1.394565642024297 115 24 1.9130001163381607 115 25 1.576561854528407 115 26 1.1185968659401766 115 27 1.4879126745622528 115 28 1.1370643903641309 115 29 0.9003046739527676 115 30 1.585857959073472 115 31 0.9504966692578734 115 32 0.6523498141642818 115 33 0.7310881887578162 115 34 1.3863906611056152 115 35 0.8710821445662376 115 36 2.343667586148115 115 37 2.2906667896879966 115 38 2.1413447830109646 115 39 2.9246452037964197 115 40 5.913019869577898 115 41 5.247265980122634 115 42 0.5547909648129625 115 43 -6.2965983526782345 115 44 -6.935408459190004 115 45 -1.2403106194290245 115 46 -7.038896135063613 115 47 -6.084161888915435 115 48 -6.650833452869186 115 49 -14.394190763278504 115 50 -5.907620131215527 115 51 -4.780745598782893 115 52 -3.777468891947006 115 53 -5.425386284036968 115 54 -7.907808408705762 115 55 -3.9468845842680267 115 56 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0.39224142297672415 119 25 0.03198861954421517 119 26 1.1171216288485013 119 27 1.4482920212429704 119 28 1.3153573303009054 119 29 1.7751202693163108 119 30 2.372791573404342 119 31 0.6451225912810514 119 32 -3.687586961388927 119 33 1.2573263341740386 119 34 0.18006821642700288 119 35 0.37708846701091403 119 36 1.2336570487403193 119 37 1.4957247454308842 119 38 1.7268031602501637 119 39 2.442874919286414 119 40 5.13873114460424 119 41 1.2923660854967132 119 42 -2.9061152522576386 119 43 -11.033584654048099 119 44 -8.653005930212132 119 45 -5.482249502463798 119 46 -16.10949676730808 119 47 -14.646437968999733 119 48 -13.565901946019872 119 49 -13.327594345997156 119 50 -12.923847739223957 119 51 -10.595919466011555 119 52 -10.451862990998638 119 53 -11.69809439784097 119 54 -12.414025479306394 119 55 -10.217695964668447 119 56 -8.00113012567573 120 1 -0.5291081679864575 120 2 0.42305940246195206 120 3 0.20605442455808287 120 4 0.8848800365209613 120 5 1.1930338184540759 120 6 1.092873693855843 120 7 -0.06287610303230906 120 8 -0.18270343692574897 120 9 0.2651938476833138 120 10 0.40149409518038937 120 11 -5.223060880249239 120 12 -9.656092704506726 120 13 -14.460206148983492 120 14 -14.980000556947324 120 15 -15.185715012990663 120 16 -18.489913417481873 120 17 -15.565727879783264 120 18 -17.068899798635325 120 19 -9.062984471152529 120 20 -14.704074396980193 120 21 -6.843089821669878 120 22 -4.6817444749435 120 23 -3.8566672346880324 120 24 -9.570835500100198 120 25 -4.518780611225015 120 26 0.5155831673100408 120 27 0.6298304827814292 120 28 -0.2815657466221708 120 29 0.4474279616240011 120 30 1.3420223426351114 120 31 -0.993338947180483 120 32 -11.150663884465853 120 33 -0.5195967427490373 120 34 -0.87223947588069 120 35 -0.2552192252967771 120 36 -0.6124967974135274 120 37 -0.6504291007229597 120 38 0.25449546794246913 120 39 0.4690287654402603 120 40 4.214115759988854 120 41 -3.6691723760417503 120 42 -6.815346021488406 120 43 -12.273584654048097 120 44 -10.089929007135208 120 45 -10.494557194771488 120 46 -17.98026599807731 120 47 -16.515668738230502 120 48 -15.425901946019874 120 49 -18.395286653689464 120 50 -15.060770816147034 120 51 -14.217457927550017 120 52 -14.378016837152485 120 53 -14.639632859379432 120 54 -14.284794710075625 120 55 -13.1515421185146 120 56 -10.825745510291112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 433.0 5 33.0 6 38.0 7 55.0 8 102.0 9 141.0 10 84.0 11 95.0 12 95.0 13 122.0 14 148.0 15 200.0 16 294.0 17 356.0 18 418.0 19 514.0 20 669.0 21 854.0 22 1044.0 23 1272.0 24 1492.0 25 1621.0 26 1740.0 27 1859.0 28 2223.0 29 2467.0 30 2970.0 31 3660.0 32 4055.0 33 4692.0 34 5489.0 35 6832.0 36 8407.0 37 9472.0 38 8214.0 39 4587.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.26122815932456 0.6879568463432749 0.033876662888115805 0.016938331444057902 8 99.36416109656152 0.5342089147741339 0.05081499433217371 0.05081499433217371 9 97.52700360916755 1.4579994527615996 0.5589649376539109 0.45603200041694353 10 45.660529778889625 33.53268446494417 13.237957497817563 7.568828258348644 11 34.457777951504255 34.39784231716374 16.391093043557568 14.753286687774434 12 33.36199820193097 13.989758824219209 21.0295899620842 31.618653011765623 13 22.138538517669133 15.322370478473887 25.341394767017615 37.19769623683936 14 22.416084645053687 18.56562076514125 32.70613989367247 26.312154696132595 15 30.957852405076515 17.592834386587977 25.50791246670497 25.941400741630545 16 34.51184376914754 18.34773879828438 23.28601040322265 23.85440702934543 17 26.686992664399533 20.345541961458782 28.537179637519706 24.430285736621975 18 25.890891086528818 21.42568632816063 27.586027179507226 25.097395405803336 19 31.8095859145745 24.029996739484837 20.135637430714056 24.024779915226606 20 26.7954252031142 28.304273548858255 20.293162582647593 24.607138665379953 21 25.746263794968012 21.754029368460827 28.891581649272304 23.608125187298857 22 26.219234126829015 19.07516710315444 24.13842525635513 30.567173513661416 23 26.00294466377412 21.411353893861808 22.68172875216615 29.903972690197918 24 25.403588320368996 21.479107219638042 29.31764583251899 23.799658627473974 25 31.542601013720407 21.250342032913338 23.542288297913924 23.66476865545233 26 23.403700807922856 20.091217096690123 27.30258014073495 29.20250195465207 27 24.104918545957297 17.934236784097877 27.060480767976625 30.900363901968202 28 23.571540388227927 25.537466082237533 28.07608015028178 22.814913379252765 29 30.965572082230707 19.209761311937008 28.572173482290676 21.252493123541605 30 23.106796116504853 17.629504137616472 37.977454877174694 21.28624486870398 31 23.280850841979042 23.555862572337208 32.6833845993431 20.47990198634065 32 30.9326620844365 17.002073741734378 32.18212408540164 19.883140088427478 33 23.925299506694856 17.546263670294678 34.61749275702764 23.910944065982825 34 20.5240516229957 23.494981097640462 38.09151349237388 17.88945378698996 35 20.45780431212034 19.413666345126053 44.19141225851865 15.93711708423495 36 20.887156692779957 25.668365551311968 35.972470247793844 17.47200750811424 37 21.35406467447356 19.213044556717453 41.761641796890295 17.671248971918693 38 27.8468774496995 20.474261823882937 35.15155474261824 16.52730598379932 39 22.454042197618552 18.60638186755797 34.611969918529354 24.32760601629413 40 15.95045334662975 14.253847869730091 36.66137434228654 33.13432444135361 41 14.397771617022952 14.031192106058416 34.567528150415846 37.00350812650278 42 23.29162154050915 18.01871158839725 37.19091300546734 21.498753865626263 43 16.658178256611166 14.679725759059744 42.80117531831537 25.860920666013715 44 17.216496195502703 15.639692497086056 35.72036617467947 31.423445132731775 45 19.77410720114905 17.734543317882093 43.296990272246525 19.194359208722332 46 18.411363814397994 19.069378802517168 36.83317231114709 25.686085071937747 47 17.597528707915693 23.228320798738284 38.1798985936054 20.99425189974062 48 19.671895800161835 23.867818642165435 38.16005846884707 18.300227088825665 49 16.661211986332752 19.530810216398077 33.166638302328934 30.64133949494024 50 24.308419019781827 18.50044365572316 37.40800668093324 19.783130643561773 51 19.28485545506822 24.921585027968007 37.55946468712426 18.234094829839513 52 18.657690099681645 19.444444444444446 41.54402171076666 20.353843745107252 53 18.69190600522193 18.612271540469973 43.04960835509139 19.64621409921671 54 18.133138297177634 23.389972181226902 35.226664228714725 23.250225292880742 55 18.537241685607544 25.035922355461505 36.102620372547484 20.324215586383467 56 24.806758238888598 19.81119757664386 34.531519298062356 20.850524886405182 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 2.0 9 3.0 10 14.0 11 25.0 12 73.5 13 187.5 14 253.0 15 282.0 16 311.0 17 360.5 18 410.0 19 489.5 20 569.0 21 635.0 22 827.0 23 953.0 24 1157.5 25 1362.0 26 1613.5 27 1865.0 28 2144.5 29 2577.0 30 2730.0 31 2795.5 32 2861.0 33 2678.5 34 2496.0 35 2117.5 36 1739.0 37 1548.5 38 1234.0 39 1110.0 40 1094.5 41 1079.0 42 1161.0 43 1243.0 44 1412.5 45 1582.0 46 2598.0 47 3533.0 48 3452.0 49 2907.5 50 2363.0 51 2433.0 52 2503.0 53 2679.5 54 2923.5 55 2991.0 56 3236.5 57 3482.0 58 3713.0 59 3944.0 60 4462.0 61 4980.0 62 4934.0 63 4486.0 64 4084.0 65 3725.5 66 3367.0 67 2981.5 68 2596.0 69 2355.0 70 2114.0 71 1819.5 72 1334.0 73 1143.0 74 957.0 75 771.0 76 605.0 77 439.0 78 331.5 79 175.5 80 127.0 81 93.0 82 59.0 83 38.0 84 17.0 85 13.5 86 10.0 87 6.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.006514742863099194 14 0.006514742863099194 15 0.20977472019179402 16 0.05472384005003322 17 0.0 18 0.0 19 0.09641819437386806 20 0.08860050293814903 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0026058971452396773 26 0.011726537153578548 27 0.1042358858095871 28 0.121174217253645 29 0.04951204575955387 30 0.01824128001667774 31 0.032573714315495966 32 0.09902409151910774 33 0.16026267443224018 34 0.05081499433217371 35 0.04560320004169435 36 0.04169435432383484 37 0.1954422858929758 38 0.2723162516775463 39 0.20456292590131467 40 0.7009863320694731 41 0.8338870864766968 42 0.14593024013342193 43 0.22671305163585193 44 0.5107558404669768 45 0.21368356590965354 46 0.201957028756075 47 0.03648256003335548 48 0.1654744687227195 49 0.4716673832883816 50 0.14723318870604177 51 0.30358701742042243 52 0.13680960012508306 53 0.19413933732035596 54 0.23713664021681063 55 0.2540749716608685 56 0.20977472019179402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 76749.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.10711540215507 #Duplication Level Percentage of deduplicated Percentage of total 1 85.92553230476051 61.0991674158621 2 8.152233664382306 11.593636399171325 3 2.6477809946128197 5.6482820623070005 4 1.191043353978085 3.3876662888115803 5 0.6596547806647854 2.3453074307157094 6 0.34448638545827687 1.469725989915178 7 0.25103529153076554 1.2495276811424254 8 0.15941657199398981 0.9068522065434077 9 0.10444534027192437 0.6684126177539772 >10 0.5295561989225639 6.045681376956051 >50 0.018323743907355153 0.929002332277945 >100 0.01465899512588412 2.5133877965836686 >500 0.0 0.0 >1k 0.001832374390735515 2.1433504019596348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1645 2.1433504019596348 No Hit CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 433 0.5641767319443902 No Hit CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 408 0.5316030176288942 No Hit CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 404 0.5263912233384149 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 171 0.22280420591799244 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 149 0.19413933732035596 No Hit CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 126 0.1641715201500997 No Hit CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 119 0.1550508801417608 No Hit CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 119 0.1550508801417608 No Hit CACAAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 89 0.11596242296316565 No Hit CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 86 0.11205357724530614 No Hit CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 77 0.10032704009172759 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0013029485726198387 0.0 0.0 0.0 0.0 6 0.0013029485726198387 0.0 0.0 0.0 0.0 7 0.0013029485726198387 0.0 0.0 0.0 0.0 8 0.0026058971452396773 0.0 0.0 0.0 0.0 9 0.0026058971452396773 0.0 0.0 0.0 0.0 10 0.01824128001667774 0.0 0.0 0.0 0.0 11 0.02736192002501661 0.0 0.0 0.0 0.0 12 0.02736192002501661 0.0 0.0 0.0 0.0 13 0.02996781717025629 0.0 0.0 0.0 0.0 14 0.035179611460735644 0.0 0.0 0.0 0.0 15 0.035179611460735644 0.0 0.0 0.0 0.0 16 0.03908845717859516 0.0 0.0 0.0 0.0 17 0.042997302896454676 0.0 0.0 0.0 0.0 18 0.044300251469074514 0.0 0.0 0.0 0.0 19 0.04560320004169435 0.0 0.0 0.0 0.0 20 0.052117942904793546 0.0 0.0 0.0 0.0 21 0.05863268576789274 0.0 0.0 0.0 0.0 22 0.06254153148575226 0.0 0.0 0.0 0.0 23 0.07035922292147129 0.0 0.0 0.0 0.0 24 0.07557101721195064 0.0 0.0 0.0 0.0 25 0.08208576007504984 0.0 0.0 0.0 0.0 26 0.09511524580124822 0.0 0.0 0.0 0.0 27 0.10032704009172758 0.0 0.0 0.0 0.0 28 0.10684178295482677 0.0 0.0 0.0 0.0 29 0.10944768010006645 0.0 0.0 0.0 0.0 30 0.11335652581792596 0.0 0.0 0.0 0.0 31 0.11596242296316564 0.0 0.0 0.0 0.0 32 0.11726537153578548 0.0 0.0 0.0 0.0 33 0.11987126868102516 0.0 0.0 0.0 0.0 34 0.121174217253645 0.0 0.0 0.0 0.0 35 0.1250830629715045 0.0 0.0 0.0 0.0 36 0.1250830629715045 0.0 0.0 0.0 0.0 37 0.1250830629715045 0.0 0.0 0.0 0.0 38 0.12638601154412435 0.0 0.0 0.0 0.0 39 0.12638601154412435 0.0 0.0 0.0 0.0 40 0.1276889601167442 0.0 0.0 0.0 0.0 41 0.12899190868936403 0.0 0.0 0.0 0.0 42 0.1315978058346037 0.0 0.0 0.0 0.0 43 0.13290075440722354 0.0 0.0 0.0 0.0 44 0.13550665155246322 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGCCCG 15 0.008172053 50.3505 36 AAGCCGC 15 0.008193415 50.317287 35 TAGAGCG 15 0.008214818 50.284115 34 GCAAGCG 15 0.008214818 50.284115 49 CGATTCA 40 2.4974725E-9 50.284115 49 ATATGCC 70 0.0 50.284115 34 ATTCCTT 15 0.008214818 50.284115 47 ACGATTC 40 2.4974725E-9 50.284115 48 ATTGACC 15 0.008214818 50.284115 34 CTGTGAT 15 0.008214818 50.284115 34 CCCTGAT 15 0.008236264 50.250988 45 TCCAAGA 15 0.008236264 50.250988 45 GATTCAG 45 1.2914825E-10 50.151875 50 ACGGGTA 15 0.008344114 50.086018 33 TATTGAC 15 0.008344114 50.086018 33 CTATTGA 15 0.008409326 49.987553 32 TCGAGCG 15 0.008409326 49.987553 32 GGCTATA 15 0.008431148 49.954815 28 AATTGAC 20 4.1778013E-4 49.95481 28 GAATAGC 30 1.0461481E-6 49.922123 13 >>END_MODULE