FastQCFastQC Report
Fri 17 Jun 2016
SRR1526264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26782
Sequences flagged as poor quality0
Sequence length56
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4721.762377716376671No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1500.5600776641027556No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1180.44059442909416774No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG970.3621835561197819No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.18295870360690017No Hit
AGGACTGGGAGATATCAACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTT320.11948323500858785No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT310.11574938391456949No Hit
AGGACTGGGGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.11201553282055113No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG280.10454783063251437No Hit
AGGACTGGGAGGTCTACGCCTGCGTCCGGCTAGCGCACAACCTGGATCCGCCAATC280.10454783063251437No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG270.10081397953849601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATT354.8899892E-850.18233547
ACGATTA354.8899892E-850.18233548
CAACGAT354.8899892E-850.18233546
TCAAAGA550.050.1823345
AAGATTA550.050.1823348
GATCCGC150.00822217750.1823345
GCTTGCC150.00822217750.1823338
CAAAGAT550.050.1823346
CTTGCCT150.00822217750.1823339
TCCGCCA150.00822217750.1823347
ATCCGCC150.00822217750.1823346
AAAGATT550.050.1823347
CTCAAAG550.050.1823344
TTGCCTC150.00822217750.1823340
AGGAGGC150.00822217750.1823347
GGCCGGT150.00828360550.088189
GGTCTCA150.00828360550.088189
GGTTTCT150.00828360550.088189
TTCCCCG150.00828360550.0881811
TACGCCT150.00828360550.0881815