##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526264_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26782 Sequences flagged as poor quality 0 Sequence length 56 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.669255470091855 39.0 38.0 40.0 34.0 40.0 2 36.696549921589124 39.0 37.0 40.0 31.0 40.0 3 36.532335150474196 39.0 37.0 40.0 30.0 40.0 4 37.15402135762826 39.0 38.0 40.0 33.0 40.0 5 36.6865805391681 39.0 38.0 40.0 30.0 40.0 6 36.92069300276305 39.0 38.0 40.0 32.0 40.0 7 37.249197222014786 39.0 38.0 40.0 33.0 40.0 8 37.113583750280036 39.0 38.0 40.0 33.0 40.0 9 37.09491449480995 39.0 38.0 40.0 33.0 40.0 10 36.73366440146367 39.0 37.0 40.0 32.0 40.0 11 36.21156000298708 38.0 35.0 40.0 30.0 40.0 12 34.16809797625271 38.0 33.0 39.0 21.0 40.0 13 33.87514001941602 38.0 33.0 39.0 20.0 40.0 14 33.95325218430289 38.0 33.0 39.0 20.0 40.0 15 33.81999103875737 38.0 33.0 39.0 19.0 40.0 16 33.29721454708386 38.0 31.0 39.0 19.0 40.0 17 33.3740945411097 38.0 31.0 39.0 20.0 40.0 18 32.88619221865432 37.0 30.0 39.0 18.0 40.0 19 33.7599880516765 37.0 31.0 39.0 23.0 40.0 20 33.741393473228285 38.0 31.0 39.0 23.0 40.0 21 34.521693674856245 38.0 33.0 39.0 24.0 40.0 22 34.28593831677993 38.0 33.0 39.0 24.0 40.0 23 34.57631991636173 38.0 33.0 39.0 25.0 40.0 24 34.223470987977 38.0 33.0 39.0 23.0 40.0 25 34.37917257859757 38.0 33.0 39.0 24.0 40.0 26 35.118363079680385 38.0 35.0 39.0 27.0 40.0 27 35.0041819132253 38.0 35.0 39.0 27.0 40.0 28 34.87924725561945 38.0 35.0 39.0 27.0 40.0 29 34.77903069225599 38.0 34.0 39.0 26.0 40.0 30 34.57523709954447 38.0 34.0 39.0 25.0 40.0 31 35.0922634605332 38.0 35.0 39.0 28.0 40.0 32 34.70308416100366 38.0 34.0 39.0 26.0 40.0 33 34.88081547307893 38.0 35.0 39.0 27.0 40.0 34 34.80834142334404 38.0 35.0 39.0 27.0 40.0 35 34.71895302815324 38.0 35.0 39.0 27.0 40.0 36 33.91184377567023 37.0 33.0 39.0 26.0 40.0 37 33.624822642073035 36.0 33.0 39.0 25.0 40.0 38 33.44544843551639 36.0 32.0 39.0 24.0 40.0 39 33.20188932865357 36.0 32.0 39.0 23.0 40.0 40 29.715256515570157 35.0 24.0 39.0 8.0 40.0 41 26.350683294750205 32.0 15.0 38.0 5.0 39.0 42 30.871144798745426 35.0 29.0 38.0 10.0 40.0 43 29.161675752370996 34.0 26.0 38.0 4.0 39.0 44 29.212530804271527 34.0 26.0 38.0 4.0 39.0 45 30.476364722574864 35.0 29.0 38.0 4.0 40.0 46 30.242476290045552 35.0 28.0 38.0 4.0 40.0 47 29.752968411619744 35.0 27.0 38.0 4.0 40.0 48 29.929355537301173 35.0 27.0 38.0 4.0 40.0 49 27.175752370995443 34.0 15.0 38.0 4.0 40.0 50 29.509670674333506 35.0 27.0 38.0 4.0 40.0 51 29.850608617728327 35.0 28.0 39.0 4.0 40.0 52 29.569598984392503 35.0 27.0 39.0 4.0 40.0 53 29.218467627511014 35.0 26.0 39.0 4.0 40.0 54 28.13766708983646 35.0 20.0 39.0 4.0 40.0 55 28.531476364722575 35.0 24.0 39.0 4.0 40.0 56 27.533978044955568 35.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.5259777959871457 1 2 1.7404667904395126 1 3 1.814282863099173 1 4 -0.09640080080902891 1 5 2.000048967645469 1 6 1.5888853571282127 1 7 1.2922821848473447 1 8 1.423597935713964 1 9 1.485494144550188 1 10 0.228896776602177 1 11 -3.0751480612249225 1 12 -17.618417516902745 1 13 -10.787422309002629 1 14 -6.725585272631157 1 15 -8.169350530184694 1 16 -23.204572177362238 1 17 -5.837379858831838 1 18 -13.494675590531994 1 19 -6.605746336280376 1 20 -6.3980549618527505 1 21 -7.736483905664528 1 22 -3.3844585458317695 1 23 -3.6942515151219624 1 24 -4.378378484528234 1 25 -3.8415859298118846 1 26 0.9666622213445848 1 27 1.562580997933388 1 28 1.9361655312109392 1 29 2.0978337329435846 1 30 1.4514408561692775 1 31 1.5012718433601506 1 32 1.9810438656145877 1 33 1.1999229761935553 1 34 1.1552461488490522 1 35 1.2542550647938029 1 36 1.4843159073341496 1 37 1.0008645494233903 1 38 1.3611922205270588 1 39 1.4115490990770994 1 40 6.402241790491114 1 41 -7.118942200163271 1 42 -8.118456436620328 1 43 -15.185232357892549 1 44 -22.16362948658954 1 45 -8.425635618104721 1 46 -14.722314947799 1 47 -23.391042770020626 1 48 -11.691303967794738 1 49 -19.81940242747241 1 50 -11.312179263165817 1 51 -8.691230291241926 1 52 -8.072589322870783 1 53 -8.243007563044841 1 54 -7.369680152489291 1 55 -7.577335600265588 1 56 -2.7131255996554486 2 1 1.292025093284444 2 2 2.8308384120611336 2 3 2.54316799823431 2 4 1.694984334326108 2 5 2.4932922108887112 2 6 2.589729951722809 2 7 2.1267416443068043 2 8 2.2453884762545044 2 9 2.496473874279914 2 10 2.4907211009265 2 11 1.647824911748053 2 12 -4.6192621114973385 2 13 -2.474077714408036 2 14 3.806509321963439 2 15 3.14146028062612 2 16 2.672961606421545 2 17 3.9312012222492427 2 18 -1.7151147797211834 2 19 2.93901717723314 2 20 3.291978821931032 2 21 3.197637715957093 2 22 2.9820955082222866 2 23 1.875849836229392 2 24 1.335303947904201 2 25 2.8163532593773013 2 26 3.2512905997229637 2 27 3.284709376311767 2 28 2.480929044724455 2 29 3.1831377869976407 2 30 2.644853018331439 2 31 3.2250894109277155 2 32 3.039320892641612 2 33 2.9955310843016605 2 34 2.743083986686891 2 35 2.4721604701992064 2 36 3.187863204631448 2 37 2.805763198072036 2 38 2.762374652959494 2 39 3.7071572071852046 2 40 6.592275574274897 2 41 -1.492253010974082 2 42 -3.98501049067438 2 43 -6.9664823578925485 2 44 -10.188967324427377 2 45 -3.1241153478344508 2 46 -8.73160548833954 2 47 -17.325164391642247 2 48 -6.452283697524468 2 49 -9.614165940985924 2 50 -6.121300884787438 2 51 -1.3939329939446274 2 52 -1.684075809357271 2 53 -1.6872643198015993 2 54 -1.5580247470838842 2 55 -1.9345991137791003 2 56 0.9405906165607689 3 1 1.6433764446357912 3 2 3.4794870607097863 3 3 3.462086917153229 3 4 2.262551901893673 3 5 3.4662651838616867 3 6 3.1843245463173986 3 7 2.77539029295545 3 8 3.0021452330112623 3 9 2.820798198604244 3 10 3.2474778576832577 3 11 3.1883654522885934 3 12 6.002359510124279 3 13 5.30970606937575 3 14 5.88759040304452 3 15 6.114433253599088 3 16 5.835123768583706 3 17 6.417687708735727 3 18 6.717317652711248 3 19 5.452530690746649 3 20 5.264951794904007 3 21 4.386826905146286 3 22 3.765879292006069 3 23 4.146120106499659 3 24 5.010979623579871 3 25 4.410947853971898 3 26 4.089128437560802 3 27 3.4468715384739284 3 28 2.6971452609406654 3 29 3.858813462673311 3 30 3.8070151804936003 3 31 2.954819140657449 3 32 2.985266838587563 3 33 3.8874229761935553 3 34 3.607948851551754 3 35 3.4721604701992064 3 36 4.998674015442255 3 37 5.481438873747713 3 38 5.735347625932462 3 39 4.923373423401422 3 40 10.078762060761388 3 41 10.778017259296188 3 42 6.582557076893188 3 43 2.4389230475128585 3 44 4.7029245674645175 3 45 7.0110197873006825 3 46 4.971097214363162 3 47 0.9451058786280235 3 48 5.493662248421479 3 49 5.385834059014076 3 50 4.63545587196932 3 51 5.200661600649969 3 52 6.775383650102192 3 53 6.961384328847053 3 54 7.1176509285917895 3 55 6.551887372707387 3 56 7.048698724668874 4 1 1.6375849388442916 4 2 2.828264409487133 4 3 3.1223185773848883 4 4 2.0360396753814456 4 5 3.1071918247883232 4 6 2.8284686904615484 4 7 2.7149012324663957 4 8 2.395324126190154 4 9 2.6953155731216185 4 10 3.0473491575545566 4 11 3.603423367346508 4 12 5.332475340240109 4 13 5.50275626242594 4 14 5.3071528226069375 4 15 4.783030422196255 4 16 5.579654013113952 4 17 6.159643950691972 4 18 6.402645838039437 4 19 4.925503663719624 4 20 4.484385514337724 4 21 3.433158951478326 4 22 4.261374787501566 4 23 3.2149746753542274 4 24 4.450490563090817 4 25 3.960497403521451 4 26 3.444340792773154 4 27 3.5253786169810084 4 28 3.157891721687129 4 29 3.9624170662769203 4 30 3.5303099037883285 4 31 3.133712319550625 4 32 3.678960532281252 4 33 3.4112325000030808 4 34 3.333817577420483 4 35 3.876278874317613 4 36 4.70008971685796 4 37 4.395209787518631 4 38 4.306132696717533 4 39 5.099049099077099 4 40 9.356110838110165 4 41 11.270938752217678 4 42 6.783329277665388 4 43 9.319231927821736 4 44 8.482203846743797 4 45 7.748471524752425 4 46 7.808935052201001 4 47 6.01074294426509 4 48 5.872684127443357 4 49 9.589823763003778 4 50 6.166987403500848 4 51 5.6182935182818845 4 52 6.873839248557786 4 53 6.474254341717064 4 54 7.107998418939278 4 55 6.578914399734412 4 56 8.700564876535026 5 1 1.7315699012503032 5 2 2.8934273167553 5 3 2.7764539157307553 5 4 2.007217620243601 5 5 2.907943704487572 5 6 2.5214511466018976 5 7 2.1146506058999748 5 8 2.5732689883455464 5 9 2.3983230919186056 5 10 2.0085020397600744 5 11 2.5695887808803377 5 12 5.370069325202515 5 13 5.1155382173131585 5 14 5.2908620957898975 5 15 5.5674915750784635 5 16 5.915493612111447 5 17 5.560646456957635 5 18 6.158284935783794 5 19 5.109714190035412 5 20 5.166089774989352 5 21 3.523384515388102 5 22 4.059620401536648 5 23 2.761340590141195 5 24 4.166029410208608 5 25 3.7061114386091702 5 26 3.29396485292353 5 27 3.5642257347754907 5 28 2.993731320684624 5 29 3.0501363645225297 5 30 3.8774276982745377 5 31 3.2101534223075205 5 32 2.301767549825115 5 33 3.097949291983028 5 34 2.2736672014806345 5 35 3.9088603279517002 5 36 3.8905659073341496 5 37 4.578167181002335 5 38 4.356258010000744 5 39 5.441154362234997 5 40 8.571649685227957 5 41 11.660663062994626 5 42 7.0427277739059875 5 43 9.559833431581136 5 44 8.990975776568359 5 45 7.500351224000539 5 46 8.045777157464158 5 47 7.7438256510320045 5 48 7.540603926942104 5 49 9.425663362001274 5 50 8.184531263149971 5 51 7.519296024547547 5 52 7.749779098181847 5 53 8.385281910639371 5 54 9.168148794879126 5 55 8.105230189208097 5 56 8.619111242449812 6 1 1.9090135102728567 6 2 2.866359647582371 6 3 2.408032863099173 6 4 1.2455634849052544 6 5 2.8214775390740385 6 6 2.4808496428425 6 7 2.410139327704485 6 8 2.3048479357139655 6 9 2.3096012874073324 6 10 2.5378253480307507 6 11 2.7034233673465096 6 12 5.0324753402401114 6 13 4.159899119568799 6 14 5.4119147273688455 6 15 3.7877923269581615 6 16 5.403463536923475 6 17 5.278691569739593 6 18 5.931217266610862 6 19 4.454075092291056 6 20 5.422480752432961 6 21 4.004587522906903 6 22 4.213755739882515 6 23 3.181641342020896 6 24 4.502871515471767 6 25 4.398592641616688 6 26 3.3300550784874403 6 27 3.077759569361959 6 28 2.8245583883537932 6 29 3.500512304372158 6 30 1.8255479990264192 6 31 2.8289504147887214 6 32 3.2122938656145905 6 33 2.6302801190506955 6 34 1.7623890059919063 6 35 2.6381836362223723 6 36 2.433423050191294 6 37 3.471400263709107 6 38 2.958513649098485 6 39 3.6276205276485314 6 40 8.43706321906254 6 41 5.061414942693872 6 42 6.0119007062368155 6 43 7.290660499250311 6 44 6.248870513410463 6 45 5.605614381895279 6 46 3.580363623629573 6 47 4.639314372836516 6 48 4.163160317919548 6 49 6.0422047153847345 6 50 3.728892165405611 6 51 3.423055423043788 6 52 3.74526781998636 6 53 3.4409210083837287 6 54 5.093712704653566 6 55 3.7217715425915543 6 56 4.238660114630267 7 1 1.5375849388442901 7 2 2.723502504725225 7 3 3.0651757202420313 7 4 1.9598491991909697 7 5 3.335763253359758 7 6 3.1094210714139265 7 7 2.495853613418774 7 8 2.561990792856818 7 9 1.766744144550188 7 10 2.9378253480307492 7 11 3.5034233673465067 7 12 5.0324753402401114 7 13 5.3313276909973695 7 14 5.2690575845117 7 15 4.8735066126724504 7 16 6.517749251209189 7 17 5.878691569739594 7 18 6.216931552325146 7 19 4.9969322351481935 7 20 5.022480752432962 7 21 4.0331589514783275 7 22 4.8708985970253735 7 23 4.6959270563066084 7 24 4.588585801186049 7 25 4.4271640701881125 7 26 3.8443407927731528 7 27 3.792045283647674 7 28 4.13884410263951 7 29 3.1576551615150166 7 30 4.196976570454993 7 31 2.8289504147887214 7 32 4.2694367227574475 7 33 3.8874229761935553 7 34 3.076674720277623 7 35 3.4096122076509445 7 36 3.661994478762722 7 37 3.442828835137675 7 38 3.5870850776699186 7 39 3.77047767050567 7 40 7.922777504776828 7 41 0.8042720855510126 7 42 6.069043563379672 7 43 7.290660499250311 7 44 7.077441941981892 7 45 6.005614381895278 7 46 6.523220766486716 7 47 7.410742944265088 7 48 7.448874603633833 7 49 10.156490429670448 7 50 6.871749308262757 7 51 5.394483994472363 7 52 7.373839248557786 7 53 7.612349579812303 7 54 8.350855561796426 7 55 6.778914399734415 7 56 6.324374400344553 8 1 0.7785115797709281 8 2 2.9659735471962705 8 3 3.2999247549910677 8 4 1.6679573072990763 8 5 2.4662651838616867 8 6 2.5627029246957775 8 7 2.721336238901401 8 8 2.6237668546328834 8 9 2.6586360364420827 8 10 2.0582886684940718 8 11 3.1883654522885934 8 12 4.921278429043198 8 13 4.715111474781153 8 14 5.401103916558029 8 15 5.168487307653145 8 16 5.429718363178303 8 17 5.012282303330323 8 18 5.663263598657192 8 19 4.830909069125028 8 20 4.967654497606709 8 21 3.630070148389528 8 22 2.4145279406547147 8 23 2.740714701094255 8 24 3.038006650606903 8 25 3.086623529647575 8 26 2.3864257348581006 8 27 3.0684931600955494 8 28 2.0755236393190444 8 29 2.0750296788895284 8 30 2.455663829142253 8 31 1.630494816333126 8 32 0.6879695412902649 8 33 2.265801354571934 8 34 1.6349758785787785 8 35 1.2018901999289398 8 36 2.0797550965233356 8 37 2.8868442791531166 8 38 2.6002124907973325 8 39 2.112562612590615 8 40 5.45714043913976 8 41 -0.4381989569200293 8 42 4.528503022839132 8 43 5.898382506972315 8 44 4.784005648545598 8 45 3.929938706219602 8 46 4.133259376525324 8 47 4.188349121871269 8 48 4.763932518691746 8 49 6.899347572527589 8 50 4.23005046656391 8 51 5.146607546595913 8 52 3.9915998663184027 8 53 3.880303247765969 8 54 3.8744076853485474 8 55 4.4167522375722506 8 56 3.508158184128334 9 1 2.1417866195165587 9 2 2.7680403198512806 9 3 3.025679921922702 9 4 2.265731552132145 9 5 2.63240190882194 9 6 2.8480765335987996 9 7 2.3731645377885187 9 8 2.381318523949261 9 9 2.766744144550188 9 10 2.519337953072764 9 11 3.5277931152456645 9 12 5.6812148360384285 9 13 5.490151220409139 9 14 5.300150021486488 9 15 6.183590646285893 9 16 5.712707234402465 9 17 5.526590729403459 9 18 6.356427350644477 9 19 5.543150722543153 9 20 5.1552538616766626 9 21 3.576016094335472 9 22 4.4868649835799985 9 23 3.6127337789956826 9 24 4.020518574295295 9 25 3.3918699525410574 9 26 3.8140886919328167 9 27 3.818095703815743 9 28 2.732121413563881 9 29 2.0702602035318165 9 30 2.7179849738163355 9 31 1.9222277257130926 9 32 3.2181762185557616 9 33 2.328599446781787 9 34 1.3170108547314072 9 35 2.566755064793803 9 36 1.8611541426282656 9 37 2.3924086670704483 9 38 3.094648102880001 9 39 3.0402255696653313 9 40 5.837903555196998 9 41 1.5244401527779061 9 42 4.1278670927914405 9 43 5.739399995048625 9 44 5.060635219292816 9 45 3.517379087777634 9 46 5.750111522789233 9 47 6.002339582920548 9 48 6.100975443969965 9 49 8.634641690174647 9 50 6.235614854481241 9 51 4.897005002875719 9 52 5.096528324188043 9 53 4.922433613425746 9 54 5.948334553393064 9 55 3.961267340910883 9 56 5.783197929756319 10 1 1.766156367415718 10 2 2.5235025047252293 10 3 3.0366042916705993 10 4 1.531277770619539 10 5 2.7071918247883247 10 6 2.766563928556785 10 7 2.6672821848473447 10 8 2.2477050785711086 10 9 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49 -16.433985760805747 114 50 -4.499679263165817 114 51 -6.274563624575258 114 52 -6.114255989537451 114 53 -7.222174229711509 114 54 -12.130096819155957 114 55 -5.087752266932256 114 56 -2.233958932988781 115 1 0.7947277959871428 115 2 2.2092167904395126 115 3 2.448032863099172 115 4 1.6798491991909685 115 5 2.1300489676454717 115 6 1.8751353571282152 115 7 1.5472821848473473 115 8 1.5448479357139675 115 9 0.8867441445501854 115 10 1.8463967766021767 115 11 0.514851938775081 115 12 -9.544667516902745 115 13 -4.748672309002629 115 14 -5.108085272631158 115 15 -7.189350530184694 115 16 -5.430822177362238 115 17 -1.269879858831839 115 18 -1.1259255905319954 115 19 -1.0144963362803772 115 20 -1.2118049618527493 115 21 3.1760160943354734 115 22 4.568041454168231 115 23 4.204498484878037 115 24 4.262871515471765 115 25 4.307164070188115 115 26 3.8329122213445856 115 27 3.666330997933386 115 28 3.167415531210942 115 29 3.3290837329435874 115 30 3.7226908561692795 115 31 2.6975218433601498 115 32 3.372293865614587 115 33 3.1274229761935572 115 34 2.9852461488490505 115 35 1.8667550647938 115 36 4.130565907334152 115 37 3.9971145494233937 115 38 4.689942220527058 115 39 4.839049099077101 115 40 9.128491790491115 115 41 8.638557799836729 115 42 3.749043563379672 115 43 -6.24648235789255 115 44 -6.1911294865895385 115 45 0.20561438189528047 115 46 -2.4310649477989976 115 47 -2.5835427700206246 115 48 -2.442553967794737 115 49 -9.220652427472412 115 50 -2.1796792631658164 115 51 -1.9312302912419241 115 52 -2.957589322870785 115 53 -3.1305075630448407 115 54 -5.323430152489291 115 55 -1.9810856002655868 115 56 1.2043744003445518 116 1 1.8947277959871442 116 2 2.9758834571061783 116 3 3.041366196432506 116 4 2.2265158658576354 116 5 2.7833823009788006 116 6 2.728468690461547 116 7 2.267282184847346 116 8 2.5715146023806312 116 9 2.3000774778835194 116 10 2.6997301099355084 116 11 -0.291814727891591 116 12 -10.924667516902744 116 13 -10.235338975669297 116 14 -2.1214186059644895 116 15 -3.636017196851359 116 16 -1.8108221773622368 116 17 -0.4832131921651701 116 18 -6.725925590531993 116 19 -0.9744963362803745 116 20 -0.2584716285194162 116 21 4.609349427668803 116 22 4.8613747875015605 116 23 4.057831818211369 116 24 4.202871515471763 116 25 4.060497403521445 116 26 4.106245554677919 116 27 3.5729976646000523 116 28 3.8340821978776063 116 29 4.062417066276915 116 30 3.516024189502609 116 31 1.7241885100268206 116 32 4.178960532281252 116 33 3.4540896428602252 116 34 3.105246148849055 116 35 3.6667550647938043 116 36 3.5905659073341525 116 37 3.2571145494233917 116 38 3.496608887193723 116 39 4.432382432410435 116 40 8.775158457157783 116 41 3.218557799836727 116 42 -3.130956436620327 116 43 -10.333149024559216 116 44 -7.784462819922872 116 45 -1.894385618104721 116 46 -3.3910649477989985 116 47 -2.0702094366872927 116 48 -2.5892206344614053 116 49 -7.300652427472411 116 50 -3.099679263165818 116 51 -0.8578969579085935 116 52 -0.5975893228707818 116 53 -2.83050756304484 116 54 -9.596763485822624 116 55 -0.4877522669322545 116 56 -0.24229226632211365 117 1 0.8280611293204814 117 2 1.8758834571061769 117 3 2.0746995297658373 117 4 0.1431825325243068 117 5 1.5833823009788048 117 6 1.9534686904615484 117 7 1.458948851514009 117 8 1.2881812690472998 117 9 0.1834108112168522 117 10 1.916396776602177 117 11 -3.5751480612249225 117 12 -6.5246675169027455 117 13 -14.110338975669297 117 14 -10.579751939297825 117 15 -8.169350530184694 117 16 -10.027488844028905 117 17 -10.23321319216517 117 18 -11.150925590531994 117 19 -4.324496336280376 117 20 -5.325138295186083 117 21 1.1176827610021363 117 22 1.5530414541682305 117 23 1.5244984848780376 117 24 2.277871515471766 117 25 1.4188307368547797 117 26 3.247912221344585 117 27 3.5729976646000523 117 28 3.1340821978776034 117 29 3.295750399610249 117 30 4.024357522835942 117 31 2.3658551766934863 117 32 2.303960532281252 117 33 1.6374229761935553 117 34 2.780246148849052 117 35 3.233421731460467 117 36 2.4738992406674853 117 37 2.490447882756726 117 38 2.996608887193723 117 39 3.6407157657437637 117 40 6.975158457157779 117 41 3.3185577998367286 117 42 -1.0976231032869954 117 43 -10.42481569122588 117 44 -14.851129486589539 117 45 -1.0610522847713888 117 46 -4.691064947798999 117 47 -4.6202094366872934 117 48 -2.5558873011280703 117 49 -11.433985760805745 117 50 -5.124679263165817 117 51 -3.524563624575258 117 52 -5.947589322870783 117 53 -5.805507563044841 117 54 -7.755096819155959 117 55 -7.171085600265588 117 56 -5.4422922663221165 118 1 0.8697277959871457 118 2 2.0842167904395126 118 3 2.283032863099173 118 4 1.059849199190971 118 5 1.9583823009788048 118 6 2.036802023794877 118 7 1.708948851514009 118 8 1.3715146023806284 118 9 1.516744144550188 118 10 2.0830634432688413 118 11 -1.0751480612249225 118 12 -9.858000850236078 118 13 -7.235338975669297 118 14 -11.163085272631157 118 15 -5.669350530184694 118 16 -13.485822177362238 118 17 -8.441546525498506 118 18 -10.984258923865326 118 19 -4.574496336280376 118 20 -3.9084716285194183 118 21 2.1176827610021363 118 22 2.761374787501566 118 23 1.816165151544702 118 24 0.6112048488051016 118 25 2.210497403521451 118 26 0.7895788880112491 118 27 3.0729976646000523 118 28 1.7174155312109392 118 29 2.0040837329435846 118 30 2.524357522835942 118 31 1.6575218433601506 118 32 -2.90437280105208 118 33 2.5540896428602196 118 34 1.4052461488490522 118 35 2.108421731460467 118 36 3.4738992406674853 118 37 3.115447882756726 118 38 3.829942220527059 118 39 4.349049099077099 118 40 7.308491790491114 118 41 5.068557799836729 118 42 -1.3476231032869954 118 43 -8.79981569122588 118 44 -8.392796153256207 118 45 -0.5610522847713888 118 46 -7.566064947798999 118 47 -6.953542770020626 118 48 -8.347553967794738 118 49 -9.39231909413908 118 50 -6.458012596499149 118 51 -2.649563624575258 118 52 -4.155922656204115 118 53 -5.097174229711509 118 54 -13.130096819155957 118 55 -2.837752266932256 118 56 -1.5256255996554486 119 1 0.78639446265381 119 2 2.000883457106177 119 3 1.9496995297658373 119 4 0.3515158658576354 119 5 1.6250489676454691 119 6 1.661802023794877 119 7 1.208948851514009 119 8 0.9548479357139641 119 9 1.1000774778835236 119 10 1.7080634432688413 119 11 -3.6584813945582546 119 12 -11.649667516902745 119 13 -13.902005642335961 119 14 -14.538085272631157 119 15 -12.044350530184694 119 16 -14.19415551069557 119 17 -16.024879858831838 119 18 -17.359258923865326 119 19 -6.866163002947044 119 20 -8.74180496185275 119 21 1.4926827610021363 119 22 2.0947081208348948 119 23 1.3994984848780376 119 24 1.402871515471766 119 25 1.835497403521451 119 26 3.122912221344585 119 27 3.4479976646000523 119 28 2.842415531210939 119 29 3.6290837329435846 119 30 2.9410241895026132 119 31 2.3658551766934863 119 32 -2.77937280105208 119 33 2.220756309526891 119 34 1.988579482182388 119 35 2.5250883981271386 119 36 3.6405659073341496 119 37 4.03211454942339 119 38 4.079942220527059 119 39 4.140715765743764 119 40 7.14182512382445 119 41 4.4852244665033965 119 42 0.15237689671300458 119 43 -10.258149024559216 119 44 -6.184462819922871 119 45 -0.4360522847713888 119 46 -12.941064947799 119 47 -8.703542770020626 119 48 -8.55588730112807 119 49 -9.475652427472411 119 50 -4.5413459298324845 119 51 -4.524563624575258 119 52 -4.155922656204115 119 53 -7.555507563044841 119 54 -9.921763485822623 119 55 -3.712752266932256 119 56 -2.8172922663221165 120 1 0.23282303408238647 120 2 1.0663596475823667 120 3 1.265175720242027 120 4 0.6074682468100221 120 5 0.9643346819311844 120 6 0.47132583331868716 120 7 0.8577583753235345 120 8 0.9905622214282488 120 9 -0.18563680783076109 120 10 0.214015824221228 120 11 -3.2775290136058715 120 12 -5.643715135950366 120 13 -5.068672309002629 120 14 -16.978561463107347 120 15 -13.812207673041836 120 16 -17.539393605933668 120 17 -15.578451287403265 120 18 -17.335449400055804 120 19 -8.431639193423234 120 20 -15.11085258090037 120 21 -6.995412477093101 120 22 -4.929101402974627 120 23 -3.237406277026725 120 24 -8.859033246432997 120 25 -3.7537883107642642 120 26 1.4919598403922052 120 27 1.3825214741238625 120 28 0.5864631502585596 120 29 1.4624170662769203 120 30 1.1493575228359418 120 31 -1.1520019661636596 120 32 -10.84484899152827 120 33 -0.5411484523778753 120 34 4.842440871470899E-4 120 35 -0.4570544590157226 120 36 0.27151828828652924 120 37 -0.08098068867185049 120 38 0.2823231729080078 120 39 1.3133348133628147 120 40 4.475158457157779 120 41 -0.538585057306129 120 42 -9.978575484239375 120 43 -14.823625215035406 120 44 -12.874939010399062 120 45 -11.061052284771389 120 46 -14.619636376370428 120 47 -16.894018960496815 120 48 -15.246363491604262 120 49 -18.95779528461527 120 50 -16.071107834594386 120 51 -13.072182672194307 120 52 -13.292827418108878 120 53 -14.597174229711507 120 54 -14.39200158106072 120 55 -13.706799885979875 120 56 -10.561339885369735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 328.0 5 82.0 6 8.0 7 45.0 8 77.0 9 50.0 10 56.0 11 28.0 12 34.0 13 47.0 14 67.0 15 61.0 16 85.0 17 91.0 18 101.0 19 161.0 20 167.0 21 206.0 22 271.0 23 330.0 24 395.0 25 502.0 26 502.0 27 586.0 28 727.0 29 843.0 30 987.0 31 1206.0 32 1357.0 33 1480.0 34 1750.0 35 2045.0 36 2632.0 37 3480.0 38 3524.0 39 2467.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.41378537823911 0.4181913225300575 0.11574938391456949 0.052273915316257184 8 98.83877230976029 0.896124262564409 0.11948323500858785 0.14562019266671644 9 93.66365469345082 4.114703905608244 1.2321708610260622 0.9894705399148681 10 34.403703980285265 38.50720633261145 15.181838548278694 11.907251138824583 11 26.323650212829513 34.88910462250765 18.19505638115152 20.592188783511315 12 31.457695467104774 15.525352848928383 24.00866253453812 29.008289149428723 13 22.264545522443797 21.084472328030472 23.45208753454328 33.19889461498245 14 28.183108057650664 20.43536703756254 28.078560227018144 23.30296467776865 15 34.80491275368831 18.6287725604733 23.91971841533738 22.64659627050101 16 30.827292429564306 24.669307226664674 21.75099020999925 22.752410133771768 17 24.796505115376 21.984915241580165 25.509670674333506 27.70890896871033 18 22.61593607646927 22.671943842879546 31.85348368307072 22.858636397580465 19 33.12904069658806 25.93146231174558 19.0179005194514 21.921596472214954 20 29.495945895452678 30.239509770952434 18.282703732765384 21.981840600829504 21 29.04189380927489 22.20521245612725 26.342319468299603 22.410574266298262 22 26.37965797923979 25.748637144350685 20.226271376297515 27.645433500112016 23 26.59248749159884 24.51646628332462 23.254424613546412 25.63662161153013 24 24.26629826002539 25.218430289000075 28.72825031737734 21.787021133597193 25 29.78977633396811 24.61446547925768 25.413539449609797 20.182218737164405 26 22.289021657953697 23.327109783420465 28.364451082897684 26.019417475728158 27 20.559874420690686 21.56525639108985 30.77814322021229 27.096725968007174 28 19.78843494187568 28.060404440623483 33.16263596605988 18.98852465144096 29 24.23234964512514 21.766903249906612 35.05042958535674 18.950317519611506 30 17.67803129317749 21.99111243885134 43.07853168527578 17.252324582695397 31 17.30848242632503 26.885294886639528 39.72285511522803 16.08336757180742 32 22.901562149637492 19.82210927573062 41.31848419164362 15.957844382988265 33 17.020640143583606 19.52961411905474 44.05848040682022 19.39126533054143 34 14.996637525218562 25.924680564895763 45.77075394156766 13.307927968318015 35 15.00467027834859 21.64020175602466 51.44778628806277 11.907341677563982 36 14.224894499010347 26.87380961272734 45.41210740560929 13.489188482653022 37 14.418221939634215 21.53195945693234 51.89063844111157 12.159180162321876 38 19.380918516300483 21.589250290077477 46.5995433619044 12.430287831717633 39 14.876898899947616 21.19658759260645 46.25084187682407 17.675671630621867 40 11.234010534236267 17.287434161023327 46.16629044394281 25.31226486079759 41 10.929847034888102 15.59415266370281 43.12787280536508 30.348127496044008 42 17.69941288657866 20.7621255749598 47.49261433753412 14.045847200927414 43 11.276675402470984 18.727068513665294 50.91351553725196 19.082740546611756 44 11.462361554345787 19.290407358738502 45.71053125586634 23.536699831049372 45 13.71764177764477 21.03845578333084 52.36794852611103 12.875953912913364 46 12.01617129594969 22.272965486261885 48.03848169499139 17.672381522797036 47 11.967280469129346 26.422141709931644 47.90273783289135 13.70783998804766 48 13.938351039952119 26.42151728265749 47.90139159060302 11.73874008678737 49 10.838307617224162 21.669106881405565 41.91538086120809 25.577204640162183 50 16.728360441203964 22.905215928210882 48.37165825387923 11.994765376705926 51 13.645552560646902 26.804432464809825 47.30083857442348 12.249176400119795 52 12.638821373817446 23.329469393860077 51.09000486108515 12.941704371237334 53 12.762773995660956 23.442058801526148 50.751851574773696 13.0433156280392 54 11.897094068304375 27.007189934092267 43.495356500898744 17.600359496704613 55 12.919645196302257 28.575171226468054 45.72775927242786 12.777424304801826 56 18.286933093848226 24.476126328393953 44.50306840293369 12.733872174824128 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 2.0 9 3.0 10 17.0 11 31.0 12 56.5 13 96.0 14 110.0 15 135.0 16 160.0 17 213.0 18 266.0 19 337.5 20 409.0 21 446.5 22 588.0 23 692.0 24 833.5 25 975.0 26 1183.5 27 1392.0 28 1539.0 29 1720.0 30 1754.0 31 1643.5 32 1533.0 33 1344.0 34 1155.0 35 991.5 36 828.0 37 718.5 38 549.5 39 490.0 40 494.0 41 498.0 42 537.5 43 577.0 44 607.0 45 637.0 46 911.5 47 1140.0 48 1094.0 49 898.5 50 703.0 51 735.0 52 767.0 53 791.0 54 792.5 55 770.0 56 829.5 57 889.0 58 935.0 59 981.0 60 1016.0 61 1051.0 62 1005.5 63 906.5 64 853.0 65 781.0 66 709.0 67 614.5 68 520.0 69 433.5 70 347.0 71 296.0 72 225.5 73 206.0 74 179.0 75 152.0 76 117.0 77 82.0 78 60.5 79 35.0 80 31.0 81 18.0 82 5.0 83 4.0 84 3.0 85 1.5 86 0.0 87 0.0 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01493540437607348 14 0.0 15 0.28377268314539617 16 0.07467702188036741 17 0.0 18 0.0 19 0.08587857516242252 20 0.07094317078634904 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00373385109401837 26 0.00746770218803674 27 0.09708012844447764 28 0.10828168172653274 29 0.044806213128220446 30 0.011201553282055111 31 0.033604659846165334 32 0.08961242625644089 33 0.14188634157269808 34 0.05974161750429392 35 0.0634754685983123 36 0.018669255470091854 37 0.16802329923082668 38 0.2427003211111941 39 0.20909566126502874 40 0.7542379209917108 41 0.896124262564409 42 0.15308789485475321 43 0.2688372787693227 44 0.5488761108207005 45 0.18669255470091853 46 0.2539018743932492 47 0.033604659846165334 48 0.18669255470091853 49 0.5414084086326637 50 0.13815249047867972 51 0.26136957658128596 52 0.14562019266671644 53 0.17922485251288178 54 0.2912403853334329 55 0.23523261892315736 56 0.21656336345306548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 26782.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.5314016877007 #Duplication Level Percentage of deduplicated Percentage of total 1 86.3433695706628 64.35292360540662 2 8.050698862782426 12.000597416175044 3 2.6501678272631635 5.925621686207154 4 1.0069635789790092 3.00201627959077 5 0.6262211312058513 2.3336569337614814 6 0.39076198587245126 1.7474423120005973 7 0.20540053103551928 1.0716152639832723 8 0.21041030008516606 1.2545739675901726 9 0.11021491909222986 0.7393025166156374 >10 0.3857522168228045 4.447016652975879 >50 0.005009769049646812 0.3621835561197819 >100 0.015029307148940433 2.7630498095735945 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 472 1.762377716376671 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 150 0.5600776641027556 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 118 0.44059442909416774 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 97 0.3621835561197819 No Hit AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49 0.18295870360690017 No Hit AGGACTGGGAGATATCAACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTT 32 0.11948323500858785 No Hit AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 31 0.11574938391456949 No Hit AGGACTGGGGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.11201553282055113 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 28 0.10454783063251437 No Hit AGGACTGGGAGGTCTACGCCTGCGTCCGGCTAGCGCACAACCTGGATCCGCCAATC 28 0.10454783063251437 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 27 0.10081397953849601 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.011201553282055111 0.0 0.0 0.0 0.0 11 0.011201553282055111 0.0 0.0 0.0 0.0 12 0.011201553282055111 0.0 0.0 0.0 0.0 13 0.011201553282055111 0.0 0.0 0.0 0.0 14 0.018669255470091854 0.0 0.0 0.0 0.0 15 0.018669255470091854 0.0 0.0 0.0 0.0 16 0.022403106564110223 0.0 0.0 0.0 0.0 17 0.029870808752146965 0.0 0.0 0.0 0.0 18 0.033604659846165334 0.0 0.0 0.0 0.0 19 0.044806213128220446 0.0 0.0 0.0 0.0 20 0.04854006422223882 0.0 0.0 0.0 0.0 21 0.052273915316257184 0.0 0.0 0.0 0.0 22 0.05600776641027556 0.0 0.0 0.0 0.0 23 0.05600776641027556 0.0 0.0 0.0 0.0 24 0.0634754685983123 0.0 0.0 0.0 0.0 25 0.06720931969233067 0.0 0.0 0.0 0.0 26 0.07094317078634904 0.0 0.0 0.0 0.0 27 0.08214472406840415 0.0 0.0 0.0 0.0 28 0.08587857516242252 0.0 0.0 0.0 0.0 29 0.09334627735045926 0.0 0.0 0.0 0.0 30 0.10828168172653274 0.0 0.0 0.0 0.0 31 0.11948323500858786 0.0 0.0 0.0 0.0 32 0.11948323500858786 0.0 0.0 0.0 0.0 33 0.1269509371966246 0.0 0.0 0.0 0.0 34 0.13068478829064298 0.0 0.0 0.0 0.0 35 0.13815249047867972 0.0 0.0 0.0 0.0 36 0.14188634157269808 0.0 0.0 0.0 0.0 37 0.14188634157269808 0.0 0.0 0.0 0.0 38 0.14188634157269808 0.0 0.0 0.0 0.0 39 0.14562019266671644 0.0 0.0 0.0 0.0 40 0.14562019266671644 0.0 0.0 0.0 0.0 41 0.14562019266671644 0.0 0.0 0.0 0.0 42 0.14935404376073483 0.0 0.0 0.0 0.0 43 0.14935404376073483 0.0 0.0 0.0 0.0 44 0.1530878948547532 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGATT 35 4.8899892E-8 50.182335 47 ACGATTA 35 4.8899892E-8 50.182335 48 CAACGAT 35 4.8899892E-8 50.182335 46 TCAAAGA 55 0.0 50.18233 45 AAGATTA 55 0.0 50.18233 48 GATCCGC 15 0.008222177 50.18233 45 GCTTGCC 15 0.008222177 50.18233 38 CAAAGAT 55 0.0 50.18233 46 CTTGCCT 15 0.008222177 50.18233 39 TCCGCCA 15 0.008222177 50.18233 47 ATCCGCC 15 0.008222177 50.18233 46 AAAGATT 55 0.0 50.18233 47 CTCAAAG 55 0.0 50.18233 44 TTGCCTC 15 0.008222177 50.18233 40 AGGAGGC 15 0.008222177 50.18233 47 GGCCGGT 15 0.008283605 50.08818 9 GGTCTCA 15 0.008283605 50.08818 9 GGTTTCT 15 0.008283605 50.08818 9 TTCCCCG 15 0.008283605 50.08818 11 TACGCCT 15 0.008283605 50.08818 15 >>END_MODULE