FastQCFastQC Report
Fri 17 Jun 2016
SRR1526263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82618
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG11941.4452056452589024No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG3480.4212157157035997No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3070.3715897262097848No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2670.32317412670362394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCC150.00798854450.64382638
CCTTAAG150.008027635550.58138341
TAAGGTA150.00820530750.3022744
CCCATTC150.00820530750.3022743
CGATTAA507.2759576E-1250.27144649
CGATTCA204.0512258E-450.27144249
TTCTAGC150.00824518350.2406634
GGTAATG150.00824518350.2406647
ACGATTC252.012346E-550.2406648
ACGATTA507.2759576E-1250.2406648
TCACTTG150.00824518350.2406634
ATTGCAT150.00824518350.2406646
TACAGCA252.012346E-550.2406647
CTGTGAT150.00824518350.2406647
CTTAAGG150.00826517650.20991542
CAGGAAA150.008446733549.93487532
GCATGTG150.008446733549.93487532
CTTAAAC150.008467087549.904513
CTCCCAT150.008467087549.904526
CGGGCGT150.008467087549.904530
Produced by FastQC (version 0.11.5)