##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526263_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82618 Sequences flagged as poor quality 0 Sequence length 56 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.326768984966954 38.0 38.0 38.0 34.0 40.0 2 36.226476070589946 38.0 36.0 38.0 31.0 40.0 3 35.62937858578034 38.0 36.0 38.0 28.0 40.0 4 35.75211213052846 38.0 36.0 38.0 30.0 40.0 5 36.24132755573846 38.0 36.0 38.0 31.0 40.0 6 34.94728751603767 36.0 34.0 39.0 28.0 40.0 7 37.54428816964826 38.0 38.0 40.0 35.0 40.0 8 37.383003703793364 38.0 38.0 39.0 34.0 40.0 9 37.34530005567794 38.0 38.0 39.0 33.0 40.0 10 36.46016606550631 38.0 35.0 39.0 31.0 40.0 11 35.24267108862475 38.0 33.0 39.0 28.0 40.0 12 33.6194412839817 38.0 32.0 39.0 20.0 40.0 13 33.412137790796194 38.0 32.0 39.0 19.0 40.0 14 33.513871069258514 38.0 32.0 39.0 20.0 40.0 15 33.510300418794934 38.0 32.0 39.0 19.0 40.0 16 33.65092352756058 38.0 32.0 39.0 21.0 40.0 17 33.66378997312934 38.0 31.0 39.0 21.0 40.0 18 33.14217240794984 37.0 31.0 39.0 19.0 40.0 19 34.00947735360333 38.0 32.0 39.0 24.0 40.0 20 34.0349802706432 38.0 32.0 39.0 24.0 40.0 21 34.9438136967731 38.0 34.0 39.0 26.0 40.0 22 34.63986056307342 38.0 33.0 39.0 25.0 40.0 23 35.20328499842649 38.0 34.0 39.0 27.0 40.0 24 34.76559587499092 38.0 33.0 39.0 25.0 40.0 25 35.03314047786197 38.0 34.0 39.0 27.0 40.0 26 35.675966496405145 38.0 35.0 39.0 29.0 40.0 27 35.59344210704689 38.0 35.0 39.0 28.0 40.0 28 35.48553583964753 38.0 35.0 39.0 28.0 40.0 29 35.34331501609819 38.0 35.0 39.0 27.0 40.0 30 35.15996514076836 38.0 35.0 39.0 27.0 40.0 31 35.616560555811084 38.0 35.0 39.0 29.0 40.0 32 35.235880800794014 38.0 35.0 39.0 28.0 40.0 33 35.46332518337408 38.0 35.0 39.0 29.0 40.0 34 35.41575685685928 38.0 35.0 39.0 29.0 40.0 35 35.26040330194389 38.0 35.0 39.0 28.0 40.0 36 34.68549226560798 38.0 34.0 39.0 28.0 40.0 37 34.40180106030163 38.0 33.0 39.0 27.0 40.0 38 34.19037013725823 37.0 33.0 39.0 27.0 40.0 39 33.744559297005495 37.0 33.0 39.0 25.0 40.0 40 30.32864508944782 35.0 26.0 38.0 10.0 40.0 41 26.99500108935099 32.0 16.0 38.0 6.0 39.0 42 31.32557069887918 35.0 30.0 38.0 16.0 39.0 43 29.74629015468784 34.0 27.0 38.0 7.0 39.0 44 29.810005083637947 34.0 27.0 38.0 6.0 39.0 45 31.04047544118715 35.0 29.0 38.0 10.0 39.0 46 30.802803263211405 35.0 29.0 38.0 4.0 40.0 47 30.36558619187102 35.0 28.0 38.0 4.0 40.0 48 30.45365416737273 35.0 28.0 38.0 4.0 40.0 49 27.826079062673994 34.0 18.0 38.0 4.0 40.0 50 30.09051296327677 35.0 28.0 38.0 4.0 40.0 51 30.32640586797066 35.0 29.0 39.0 4.0 40.0 52 30.11489021762812 35.0 28.0 39.0 4.0 40.0 53 29.655401970514898 35.0 27.0 39.0 4.0 40.0 54 28.712096637536614 35.0 23.0 39.0 4.0 40.0 55 29.174392989421193 35.0 27.0 39.0 4.0 40.0 56 27.959960299208404 34.0 20.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.8922514947816609 1 2 1.9785306910717466 1 3 2.7809204588406757 1 4 2.624387410578244 1 5 1.959204987894779 1 6 2.4519147076168935 1 7 0.9356269793223788 1 8 1.0517102624111345 1 9 1.0806170345914978 1 10 1.2872583862171183 1 11 -4.753659123988744 1 12 -15.897917041314471 1 13 -12.00689073338636 1 14 -7.952167070834083 1 15 -9.268163399555863 1 16 -23.145041197389702 1 17 -5.296519927913465 1 18 -12.439170893078416 1 19 -6.25452980663464 1 20 -5.32715959818151 1 21 -5.031272957389735 1 22 -2.5143632763437616 1 23 -3.4417243820547476 1 24 -3.791558173375556 1 25 -3.1468337755230635 1 26 2.411996752258659 1 27 2.206356216769933 1 28 1.9077297356758294 1 29 2.3058853565397754 1 30 2.9986653771058585 1 31 2.25185310870949 1 32 2.262787195649146 1 33 1.9500314429761687 1 34 1.8970241797545384 1 35 1.9216902361818953 1 36 2.596577487342522 1 37 3.0721742553968667 1 38 2.4348834278344924 1 39 2.9472491080359546 1 40 5.808263412226815 1 41 -7.886135381277327 1 42 -7.532486177581006 1 43 -16.76210499840493 1 44 -22.332476507334146 1 45 -10.106898413335053 1 46 -17.16742249855711 1 47 -23.633578141226995 1 48 -13.516536358549544 1 49 -20.970987988829272 1 50 -12.70662768056945 1 51 -8.442595140814106 1 52 -8.376594395446649 1 53 -8.560778762953476 1 54 -8.240430823771998 1 55 -8.37070178099517 1 56 -3.05585313018344 2 1 1.1362975210059076 2 2 2.4871383085902536 2 3 3.2760591619001715 2 4 3.0717159272136882 2 5 2.1120013486119333 2 6 2.418287020523863 2 7 1.4041846458998464 2 8 1.4445387344475336 2 9 1.5257155985019395 2 10 2.068544622948899 2 11 0.8426302346768537 2 12 -3.25381749622483 2 13 -2.3718893954147227 2 14 2.653934079821525 2 15 2.822194284861226 2 16 2.535807968607976 2 17 3.3165386752441464 2 18 -1.1943220838731712 2 19 2.526399637661143 2 20 2.3511028226551645 2 21 1.8995093100261968 2 22 2.145345937820899 2 23 1.701527780999399 2 24 0.5391884147967758 2 25 1.6271382162707084 2 26 1.8132990446500585 2 27 1.6975702030334219 2 28 1.9381462908448626 2 29 1.9870021168644598 2 30 2.063958392893923 2 31 1.5503991795327892 2 32 1.7637237757936504 2 33 1.937365311498155 2 34 2.242042465366886 2 35 1.2089081471621839 2 36 2.804409081312727 2 37 2.76756271316156 2 38 2.296091170230355 2 39 3.0038899072510077 2 40 6.3666435745673695 2 41 -6.29644668267774 2 42 -6.94707898821342 2 43 -14.614303731791784 2 44 -14.792292759229605 2 45 -6.132052280073079 2 46 -10.053962503908995 2 47 -17.622695971928984 2 48 -7.89206039743992 2 49 -12.571915649162872 2 50 -6.741325744970485 2 51 -1.1815122757708458 2 52 -0.9380964916022094 2 53 -0.4967345206914118 2 54 -0.1615796954159201 2 55 -1.0735828799158718 2 56 1.5412390114631584 3 1 1.6707276032620726 3 2 2.86755350639676 3 3 3.6030470193228084 3 4 3.5332122061012754 3 5 2.6248098092464787 3 6 3.761256076536398 3 7 1.7844431652888062 3 8 1.707758475928145 3 9 1.8994719635626538 3 10 2.546492135679024 3 11 2.999935365895027 3 12 4.23854443091971 3 13 3.4546675963596627 3 14 4.197294831877926 3 15 3.556546027908624 3 16 4.938471501706296 3 17 4.773647958440392 3 18 5.5090856717084975 3 19 4.400743546787659 3 20 4.636335881801273 3 21 3.530844993535794 3 22 3.7910176965361373 3 23 3.3135920191505903 3 24 4.340598520554707 3 25 3.109472724692175 3 26 2.383843502151038 3 27 2.7240488555990297 3 28 2.7627448023998937 3 29 2.4054118309263472 3 30 3.0190700262664265 3 31 2.4483231905002825 3 32 2.0579658439487645 3 33 2.2007417313963202 3 34 2.2745532370080923 3 35 2.3011133958891747 3 36 3.1556820934553045 3 37 3.3822904844110724 3 38 3.3986371945155085 3 39 4.049875022801345 3 40 7.736717996815706 3 41 8.742017868830288 3 42 4.856837972225282 3 43 0.16109775665318438 3 44 2.9418239661914676 3 45 5.0225893180467835 3 46 3.0919834420369554 3 47 -2.5649599750625534 3 48 3.3325381141151134 3 49 5.641323677119935 3 50 3.5908325002312367 3 51 5.320642052470443 3 52 5.247254076357056 3 53 5.4805471087641315 3 54 5.956555831415262 3 55 5.618622368811113 3 56 6.4327392073111795 4 1 1.5058352830670358 4 2 2.6016453389851364 4 3 3.5788292216795767 4 4 3.379453571399928 4 5 2.4300109469953597 4 6 3.3113576612580573 4 7 1.8740002559023736 4 8 1.9279941524823911 4 9 1.862332589324538 4 10 2.4427131840333516 4 11 3.7016013553302187 4 12 5.282429030675907 4 13 5.14162411809879 4 14 5.322284673405761 4 15 5.341348953548611 4 16 5.45817893770792 4 17 5.731795053117352 4 18 5.7132303283521395 4 19 5.110048707291519 4 20 4.979123366563563 4 21 3.968634509992519 4 22 4.357941711164337 4 23 3.6418325717714737 4 24 4.332065403935445 4 25 3.819021688528011 4 26 3.246826029578912 4 27 3.2018221185002886 4 28 3.369652563472627 4 29 3.434505695209161 4 30 3.06547392711974 4 31 2.837677111670537 4 32 2.6157065997390916 4 33 2.037474766747799 4 34 1.889343972481477 4 35 2.1285006368481305 4 36 3.4829474327482757 4 37 3.3031356692952656 4 38 3.5223267516061227 4 39 4.655401231659532 4 40 7.959554242244394 4 41 10.129502631122044 4 42 5.964599044959936 4 43 8.075685322683249 4 44 6.8299182368131675 4 45 5.720435721947638 4 46 5.667684376616393 4 47 5.791886835177191 4 48 5.726361763038316 4 49 8.507313112308879 4 50 6.668036888691212 4 51 5.5965461564931935 4 52 5.937461309189235 4 53 6.060392699987215 4 54 6.82360174770945 4 55 6.397411478928955 4 56 5.713925716860146 5 1 1.4259411469327148 5 2 2.4948717057041847 5 3 3.53094236368603 5 4 3.0543357151432105 5 5 2.3640941493773013 5 6 3.070682779114314 5 7 1.08440468895175 5 8 1.472283293165539 5 9 1.136255341785045 5 10 1.4135179148248511 5 11 3.0731173589692844 5 12 4.52326932510978 5 13 4.212420578275783 5 14 4.567591099673237 5 15 5.098523711633135 5 16 4.997340297396946 5 17 4.429669505189139 5 18 4.999889827152899 5 19 4.39166313124322 5 20 4.049340883725087 5 21 3.3667818923430275 5 22 3.3323028061936952 5 23 3.291737459704649 5 24 3.3540652385231553 5 25 3.3194765723258826 5 26 1.7452132597501162 5 27 2.357061552790256 5 28 2.1365039405299413 5 29 2.6025645819844456 5 30 2.523417852353383 5 31 1.166599450600316 5 32 2.2762805803858512 5 33 1.9828887110038593 5 34 1.422302371290506 5 35 1.2602515259391893 5 36 2.86810995032333 5 37 2.686561357823919 5 38 2.839422111791386 5 39 2.757377908526621 5 40 7.103628346950373 5 41 9.426665810346258 5 42 5.532790261567332 5 43 7.373179326487737 5 44 6.275952477157222 5 45 5.0793803915365885 5 46 5.334461318143148 5 47 5.366859955680038 5 48 5.430191057554897 5 49 8.483493742529703 5 50 4.967515839977295 5 51 4.622681298334232 5 52 4.952679239881487 5 53 5.173121175712961 5 54 5.358710506290212 5 55 4.853866693551399 5 56 5.047975836784055 6 1 1.306717941426328 6 2 2.396297467749413 6 3 3.3251082109492174 6 4 3.413808019305783 6 5 2.3182075548181373 6 6 2.861980714217559 6 7 1.6051355968508005 6 8 1.6046988946076937 6 9 1.5767666495529937 6 10 2.3002763546806335 6 11 3.3188564609030777 6 12 5.420106427699096 6 13 4.650883377358049 6 14 5.19222236810981 6 15 4.999621712288658 6 16 4.99201417481418 6 17 5.199171307876782 6 18 5.3990212594701745 6 19 4.61749072875223 6 20 4.4497014212537636 6 21 3.652262062778952 6 22 3.513964556439518 6 23 3.6780042574758696 6 24 3.5334326590410186 6 25 3.4757368148693075 6 26 2.753489234840252 6 27 2.3662388717020875 6 28 2.071279423977991 6 29 2.6644295442918846 6 30 2.4308252597607876 6 31 1.9212700504036633 6 32 1.186146198216072 6 33 1.8335114576443061 6 34 1.5100604833849047 6 35 1.869068307322344 6 36 1.236841513745162 6 37 2.459504655103501 6 38 2.605400479539668 6 39 2.9092953126564183 6 40 6.672949880873674 6 41 2.948298062067007 6 42 4.600718809584375 6 43 5.641452023963971 6 44 4.609859392922548 6 45 3.8416714436506467 6 46 3.93827973833325 6 47 4.296473197240186 6 48 4.139220883841364 6 49 6.873529796282575 6 50 5.20637195272721 6 51 4.968387624129054 6 52 5.011102707596994 6 53 4.951964178007291 6 54 5.420001886165661 6 55 4.132873576540586 6 56 4.440388160612308 7 1 1.5196809043892898 7 2 2.553704875156818 7 3 3.389923025764034 7 4 3.145289500787264 7 5 1.9756149622255492 7 6 2.454573306810147 7 7 1.0310615227767244 7 8 1.2250692649780603 7 9 1.4193592421455818 7 10 1.7447207991250764 7 11 2.9947823868290016 7 12 4.447884205476875 7 13 4.706438932913606 7 14 4.220000145887589 7 15 4.795918008584955 7 16 4.704977137777149 7 17 4.560282418987896 7 18 5.213836074284984 7 19 3.756379617641123 7 20 3.76451623606858 7 21 2.7541139146308 7 22 2.301001593476556 7 23 3.326152405624022 7 24 3.20009932570769 7 25 2.9572182963507885 7 26 2.3090447903958093 7 27 1.662535167998385 7 28 1.8305386832372506 7 29 2.349614729477075 7 30 2.3752697042052375 7 31 1.1342330133666252 7 32 2.149109161179034 7 33 1.2501781243109704 7 34 1.926727150051569 7 35 2.137586825840863 7 36 2.2461007730044216 7 37 2.440986136584982 7 38 1.947993072132256 7 39 3.261147164508266 7 40 5.645172103095895 7 41 -0.19059082682188233 7 42 3.5914595503251157 7 43 5.326637209149155 7 44 4.230229763292915 7 45 3.0546344066136086 7 46 4.391983442036953 7 47 5.046473197240186 7 48 4.083665328285807 7 49 6.373529796282575 7 50 4.882297878653141 7 51 4.977646883388314 7 52 4.649991596485879 7 53 5.007519733562841 7 54 5.771853738017516 7 55 4.993984687651693 7 56 4.662610382834529 8 1 1.1143699330405852 8 2 2.2636978870366278 8 3 3.0165827043105082 8 4 2.825583001835483 8 5 1.9684521390249898 8 6 2.1654398337144087 8 7 1.0913340594643586 8 8 1.2573893208830214 8 9 1.1910224147521546 8 10 1.3331205196002713 8 11 2.761379172293715 8 12 4.802531305406994 8 13 4.45294487281577 8 14 4.926499097669563 8 15 4.91593897294554 8 16 3.9991769980147396 8 17 4.274468302985099 8 18 4.665443341929986 8 19 4.187371930708906 8 20 3.537402329709394 8 21 3.1305988901723794 8 22 3.3310505103179224 8 23 2.7370538731292626 8 24 3.3721817855259957 8 25 3.0838779748972627 8 26 2.3343767261050985 8 27 2.411661652973926 8 28 2.3245987810709323 8 29 2.0398663018041177 8 30 2.476248041032626 8 31 1.5638277023952725 8 32 2.3458247446870715 8 33 2.2264185156457046 8 34 1.3606894142025112 8 35 1.6381109348555398 8 36 2.2431308219212625 8 37 1.9457031177170592 8 38 2.447119557107797 8 39 2.292943111398557 8 40 4.911943591565503 8 41 -3.119836109840751 8 42 2.7881751338331533 8 43 4.7633947213783685 8 44 4.75224234190927 8 45 2.8421955526653235 8 46 3.505190989206767 8 47 3.3060818625092274 8 48 4.201939262597474 8 49 6.020979132411156 8 50 3.8931294649564236 8 51 4.21768881210949 8 52 3.5416757334530367 8 53 4.491621760117699 8 54 4.423495946263497 8 55 3.685808587022766 8 56 4.105831906244731 9 1 1.1644276919932182 9 2 2.0159119802210697 9 3 3.0136947566786247 9 4 2.5999380268643577 9 5 1.783816020426606 9 6 1.6664024829250366 9 7 1.3060048334343222 9 8 1.0529226285457085 9 9 1.1232519103239653 9 10 1.192188675164381 9 11 2.7529078592401888 9 12 4.304649133670374 9 13 3.9681547303436844 9 14 4.622494476953349 9 15 3.8123579178820037 9 16 4.530751892123327 9 17 4.137001995707472 9 18 4.431712113589597 9 19 2.7505217189260804 9 20 3.7811451098403097 9 21 2.586124496641382 9 22 2.9124906335370255 9 23 2.7218440156013486 9 24 2.498096302276089 9 25 2.63201043542864 9 26 1.5889011773950514 9 27 1.2547498316416181 9 28 1.7678024776439045 9 29 2.1336283349192513 9 30 1.5336219339860335 9 31 1.1208165356644315 9 32 1.6211424189265813 9 33 0.41760064887635195 9 34 1.1818291908678944 9 35 1.3649110888794098 9 36 1.8524877722485584 9 37 1.9052718508706974 9 38 1.933064878632635 9 39 2.0861660609557333 9 40 4.445058724411091 9 41 3.2430826425658736 9 42 3.7165540325624598 9 43 4.780529877327538 9 44 4.961522280299722 9 45 3.130220196485112 9 46 4.647085482853278 9 47 4.205581284919699 9 48 4.345759054665248 9 49 6.446470083508572 9 50 2.9000605392729426 9 51 2.898281804023238 9 52 3.462160908655189 9 53 3.0746021220738022 9 54 4.3776738438376235 9 55 3.1130323066993135 9 56 3.518808417603992 10 1 1.1661108394790176 10 2 2.259126791918945 10 3 2.9913930524917944 10 4 2.9866411617265527 10 5 1.7882342825768234 10 6 2.97452366954402 10 7 1.341771717507541 10 8 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6.112949880873678 114 41 6.679409173178115 114 42 2.74553362439919 114 43 -0.4881776056656584 114 44 -6.763103570040418 114 45 0.9338936658728691 114 46 -4.774683224629715 114 47 -4.819452728685739 114 48 -5.67151985689938 114 49 -17.70128501853224 114 50 -6.1784428620876035 114 51 -7.817908672167242 114 52 -8.029267662773382 114 53 -9.649887673844564 114 54 -12.125183299019522 114 55 -9.346015312348303 114 56 -5.361463691239546 115 1 0.7408884132680313 115 2 2.1364040126504804 115 3 2.889288829924361 115 4 2.3637065479714323 115 5 1.8620939069236684 115 6 2.490976148007512 115 7 0.590041735866528 115 8 0.6970378088644154 115 9 0.4869645186549718 115 10 1.6348780796933156 115 11 -2.8540253249924064 115 12 -11.916905241504356 115 13 -7.474814238065594 115 14 -8.587457997186991 115 15 -10.85286433540287 115 16 -7.956108605500379 115 17 -2.764172172589987 115 18 -2.837787467064583 115 19 -2.4286787283761306 115 20 -2.3080357679902725 115 21 2.8141088410640833 115 22 2.400189822801771 115 23 2.42508695661337 115 24 3.0386330649263584 115 25 2.675762182702897 115 26 2.17193164985801 115 27 2.3423931081303024 115 28 2.474120621339736 115 29 2.019706053677986 115 30 2.2768425098876293 115 31 1.8246186044371484 115 32 1.6889366599106452 115 33 0.8724510822003637 115 34 1.7897408486817028 115 35 1.3152885001178802 115 36 2.136384892740594 115 37 2.8074244927493623 115 38 2.1792208752778706 115 39 3.7882907464463713 115 40 7.389844858042629 115 41 6.532011912904146 115 42 2.144985679193713 115 43 -5.958497240368853 115 44 -6.388491697894299 115 45 1.9782772275167062 115 46 -1.2997973798808573 115 47 -1.164840856539623 115 48 -0.6711545600957258 115 49 -12.297449402093886 115 50 -1.2054748255579213 115 51 -0.28567122924486554 115 52 0.055115898870454316 115 53 -1.1704356190500427 115 54 -5.6692928880606175 115 55 0.6629344593411943 115 56 2.2951573133266656 116 1 1.2432676490286667 116 2 2.508285771842978 116 3 3.134854799643179 116 4 3.0265760504948673 116 5 2.281560381328859 116 6 2.883910538778963 116 7 1.2997749730691268 116 8 1.5489484072782531 116 9 1.4527900413658585 116 10 1.8490092981504205 116 11 -3.1085314533269397 116 12 -15.416833143451 116 13 -9.079428513480156 116 14 -4.850760088030537 116 15 -6.733614155157738 116 16 -4.066562823236499 116 17 -2.7705168012850123 116 18 -8.073980689847566 116 19 -3.0335813960041023 116 20 -3.68265725321017 116 21 2.2319891582955194 116 22 2.450514264041857 116 23 1.8325851541620324 116 24 2.2481500079688956 116 25 2.3758342807562443 116 26 2.4717153556979525 116 27 2.4430419906104746 116 28 2.0295640243678577 116 29 2.371544554038479 116 30 2.6813125891954854 116 31 1.7604513369533663 116 32 1.9494990247267907 116 33 1.630490015149178 116 34 1.9583060974199924 116 35 2.130861679642031 116 36 2.977192390938143 116 37 2.054144031321826 116 38 2.9258683157969756 116 39 3.5075409266915045 116 40 7.448388477364901 116 41 5.106777594230749 116 42 -1.3134137440218616 116 43 -8.053089886367413 116 44 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1.6082432425793343 117 27 1.9289807180260254 117 28 1.75729379655953 117 29 1.2513560230094143 117 30 1.4637163598160683 117 31 1.6866100172361627 117 32 1.7455712949546012 117 33 1.8004821596896363 117 34 1.3446375978127634 117 35 1.0948836749287594 117 36 2.381120120710335 117 37 1.8477025544954273 117 38 1.8972744430554158 117 39 2.404320188278305 117 40 6.319716050027907 117 41 3.231050964222895 117 42 -3.170287271123197 117 43 -8.881212431536305 117 44 -7.637929440687181 117 45 -2.5890914087539976 117 46 -4.451300140052599 117 47 -4.473980091869819 117 48 -5.550524832023758 117 49 -8.045762630472538 117 50 -6.161925449152278 117 51 -6.811739517938385 117 52 -6.9600139314300975 117 53 -7.246902599217698 117 54 -13.033840015437432 117 55 -6.933876008865717 117 56 -5.723652745965914 118 1 0.6633245480329322 118 2 1.8970482185001671 118 3 2.379662765503774 118 4 2.4410852965830614 118 5 1.7876770242876105 118 6 2.1435122457490863 118 7 0.5933738350890394 118 8 0.8229171128259125 118 9 0.9131029858893314 118 10 1.3027788571831351 118 11 -4.513225621179004 118 12 -9.078141820549153 118 13 -11.142159665684993 118 14 -11.481201055313612 118 15 -8.511139048472106 118 16 -13.725453292653285 118 17 -11.18346132475585 118 18 -11.694322083873171 118 19 -5.761888650627149 118 20 -5.5227710512187045 118 21 0.5571669676838553 118 22 1.1273279198028803 118 23 0.9402665197381381 118 24 1.1067559823643407 118 25 1.0550661441986406 118 26 1.8988846302356492 118 27 1.688561194024409 118 28 1.4291372818358496 118 29 2.117632747495094 118 30 2.19909352802906 118 31 0.7170658461994606 118 32 -4.5876275755577005 118 33 0.022950897083745758 118 34 0.6294298527542708 118 35 0.5827820210360599 118 36 1.7548595317631808 118 37 1.94774289334174 118 38 2.034829908969094 118 39 1.6435295468906475 118 40 5.965742673666469 118 41 3.505355119124065 118 42 -2.6993312404656713 118 43 -9.767456884944938 118 44 -9.085085552022399 118 45 -4.7401603881811845 118 46 -12.459367909314398 118 47 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0.8485598771563971 119 30 1.637341902986293 119 31 0.11887905181013281 119 32 -3.9540319546203087 119 33 0.4398171304056717 119 34 -0.21251335627754742 119 35 -0.4598112051014738 119 36 0.8022423576270157 119 37 1.2416190479773874 119 38 1.2664412671627332 119 39 1.7848227388167501 119 40 5.867042707877893 119 41 1.1670041098869781 119 42 -3.76484612243625 119 43 -10.808852711150891 119 44 -7.93643690337375 119 45 -5.401296207544853 119 46 -15.462446937709883 119 47 -12.105191125310213 119 48 -13.026625342131446 119 49 -10.561538183089205 119 50 -10.495573663775367 119 51 -8.320524705922516 119 52 -7.286482852646799 119 53 -9.0169762814395 119 54 -9.068980767373954 119 55 -7.679011092939021 119 56 -4.701548079425198 120 1 0.03342045038689889 120 2 1.295042987462672 120 3 1.9672826434462323 120 4 1.7026371710381554 120 5 1.0810510434680793 120 6 1.714131251851967 120 7 0.04629449768712135 120 8 0.04436436653122655 120 9 0.10185625528775688 120 10 0.4950194849076368 120 11 -4.7154924040909485 120 12 -10.058089510174263 120 13 -12.06805330125605 120 14 -14.466379782427822 120 15 -13.436160844461643 120 16 -16.726325132234802 120 17 -13.920601690928475 120 18 -14.834252336706651 120 19 -7.634600071725664 120 20 -13.037156404313738 120 21 -5.489912369718063 120 22 -3.0980426120192632 120 23 -3.4838834366698848 120 24 -9.178335560791716 120 25 -3.1912285375799136 120 26 0.22451671393224615 120 27 -0.18632982602790094 120 28 -0.6449690825930929 120 29 -0.3627508619207802 120 30 0.4750307555791906 120 31 -1.0536403438615736 120 32 -11.728429667972698 120 33 -0.7808851970749302 120 34 -1.2023051080129505 120 35 -0.9385780487111077 120 36 0.4196372126698904 120 37 1.1103409752946618 120 38 0.6878377555253294 120 39 1.5759619793230826 120 40 4.474025149690881 120 41 0.46263497962972977 120 42 -2.963498633665324 120 43 -12.934414164805442 120 44 -11.613856258212458 120 45 -9.460299882514228 120 46 -17.591887525704983 120 47 -18.067624771696494 120 48 -14.555820932168196 120 49 -18.60257534111289 120 50 -14.304679421227387 120 51 -11.760704371091972 120 52 -12.222170888579832 120 53 -13.117629609328434 120 54 -15.410344589342102 120 55 -12.124294882240779 120 56 -9.602323906293307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 527.0 5 16.0 6 46.0 7 125.0 8 123.0 9 109.0 10 83.0 11 57.0 12 74.0 13 94.0 14 113.0 15 174.0 16 229.0 17 298.0 18 358.0 19 428.0 20 543.0 21 749.0 22 911.0 23 1149.0 24 1289.0 25 1497.0 26 1579.0 27 1867.0 28 2211.0 29 2607.0 30 3263.0 31 3779.0 32 4083.0 33 4817.0 34 5646.0 35 6842.0 36 9015.0 37 11244.0 38 10563.0 39 6107.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.61630637391367 0.30380788690115956 0.04720520951850686 0.032680529666658596 8 99.32218160691374 0.5386235445060399 0.07746495920985741 0.06172988937035513 9 97.01396790045753 1.9620421699871697 0.5107845747899974 0.5132053547653054 10 35.29376165000363 41.74392989421192 13.918274468033601 9.044033987750852 11 27.35118255101794 36.26812559006512 20.683144109031932 15.697547749885013 12 31.589968287782323 17.09070662567479 22.324432932290783 28.9948921542521 13 22.950740287883008 16.952168807428304 26.561989273997312 33.53510163069138 14 22.306416665657945 22.207158679626694 30.205899797852638 25.280524856862723 15 28.465700905098153 19.948557423988742 25.945742641527747 25.639999029385358 16 30.028098733134705 22.764818448271686 25.29976019184652 21.907322626747085 17 25.13253770364812 23.508194340216416 27.02921881430197 24.3300491418335 18 22.930460570114384 24.95914785450584 28.033650063547782 24.076741511831994 19 28.777762968168762 25.975111778889843 22.403034084988306 22.844091167953085 20 23.913991520290732 29.22228952150212 22.59357964869776 24.27013930950939 21 25.092594834055532 22.397056331550026 28.405432230264594 24.10491660412985 22 23.94514512575952 23.999612675203952 23.245539712895493 28.80970248614104 23 23.780532087438573 25.565857319228257 23.451306010796678 27.20230458253649 24 22.2121355169033 26.867351755691928 28.500187612718932 22.42032511468584 25 28.132905646674335 26.39109120619742 24.70374629304606 20.772256854082187 26 23.568860535776974 25.109854858429472 26.96316381992277 24.35812078587079 27 22.314980855910434 22.477342121843648 28.4750157514661 26.73266127077982 28 22.226127425434182 27.127292118626606 30.33413726654628 20.31244318939293 29 25.742166690880914 22.691171485301954 31.448254152743477 20.11840767107366 30 20.693412100815923 22.797375493305573 36.928310292230584 19.58090211364792 31 19.230489842369064 27.878398992711688 33.9124434005666 18.978667764352647 32 24.241690086248667 22.29867235197209 35.27836999709274 18.1812675646865 33 19.852085354025217 22.476964112512125 35.89233753637245 21.778612997090203 34 17.93663865139148 27.101751156538377 38.47458037638966 16.487029815680483 35 18.410791697948707 22.804000871860698 43.32905475769538 15.456152672495216 36 17.9295314202688 27.1255599951568 38.34725753723211 16.597651047342293 37 18.09770944233591 22.849798105954967 42.78577404843032 16.26671840327881 38 22.688045444062777 22.79728597958416 39.119035770206466 15.395632806146601 39 18.51092730492153 22.53620200354137 38.417784461639215 20.535086229897885 40 14.536166684954221 18.361922875291075 37.3809785060288 29.720931933725907 41 13.056694989745093 16.9218185369665 35.74689911124133 34.27458736204708 42 21.46250424160163 23.103398128847736 38.92214843181928 16.511949197731347 43 15.285882395758774 20.009947955209938 41.90515473923013 22.799014909801162 44 15.273890930328765 21.03277930547782 37.4954371790816 26.19789258511182 45 16.724671072576243 22.96731946886558 42.9527678409022 17.355241617655974 46 16.867353001806784 23.387536832914986 39.2993561060788 20.445754059199437 47 16.548208599208127 26.498686265725457 39.08510818632021 17.8679969487462 48 17.077014462183755 28.131553745257058 38.68300784327987 16.108423949279317 49 14.092032081005756 22.81938004308299 34.539413633210415 28.549174242700843 50 20.365994061685754 24.454947585287524 39.33345452341999 15.84560382960674 51 17.533381888807963 28.546977421704295 37.67540665210002 16.244234037387713 52 17.296950162973015 25.07603387900011 41.32123253644174 16.30578342158514 53 16.917612258752786 25.36490156143924 41.214479681893124 16.50300649791485 54 15.8956627236882 27.358204428268124 35.54382772217167 21.202305125872005 55 16.94103089782005 28.99809542294955 38.16796671236034 15.892906966870063 56 21.169423291458507 25.396517437066496 37.182907309501864 16.25115196197313 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 2.0 7 3.0 8 23.5 9 44.0 10 86.0 11 128.0 12 183.0 13 276.0 14 314.0 15 452.0 16 590.0 17 679.0 18 768.0 19 842.0 20 916.0 21 1133.0 22 1777.5 23 2205.0 24 2515.0 25 2825.0 26 3152.0 27 3479.0 28 3716.5 29 3860.0 30 3766.0 31 3419.5 32 3073.0 33 2791.5 34 2510.0 35 2288.5 36 2067.0 37 2048.5 38 1975.5 39 1921.0 40 2020.0 41 2119.0 42 2323.0 43 2527.0 44 3313.0 45 4099.0 46 4109.0 47 3667.5 48 3216.0 49 3198.0 50 3180.0 51 3343.0 52 3506.0 53 3521.5 54 3448.0 55 3359.0 56 3351.0 57 3343.0 58 3375.5 59 3408.0 60 3279.0 61 3150.0 62 3013.0 63 2627.0 64 2378.0 65 2037.5 66 1697.0 67 1512.0 68 1327.0 69 1154.0 70 981.0 71 818.0 72 560.0 73 465.0 74 365.0 75 265.0 76 193.5 77 122.0 78 94.0 79 45.0 80 24.0 81 15.5 82 7.0 83 6.5 84 6.0 85 3.0 86 0.0 87 1.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.018155849814810332 14 0.006051949938270111 15 0.2372364375801883 16 0.06294027935800915 17 0.0 18 0.003631169962962066 19 0.10772470890120797 20 0.0823065191604735 21 0.0 22 0.0 23 0.0 24 0.001210389987654022 25 0.003631169962962066 26 0.0108935098888862 27 0.1040935389382459 28 0.12951172867898036 29 0.026628579728388484 30 0.014524679851848264 31 0.024207799753080444 32 0.07988573918516546 33 0.16703381829625505 34 0.05325715945677697 35 0.0435740395555448 36 0.03389091965431262 37 0.1803481081604493 38 0.2796000871480791 39 0.19850395797525963 40 0.7201820426541432 41 0.8545353312837397 42 0.12345977874071026 43 0.22876370766661017 44 0.5228884746665375 45 0.18518966811106538 46 0.18276888813575734 47 0.035101309641966644 48 0.15371952843206083 49 0.545885884431964 50 0.12467016872836428 51 0.28807281706165727 52 0.10772470890120797 53 0.15735069839502286 54 0.23360526761722628 55 0.22392214771599409 56 0.18155849814810332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 82618.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.24389358251229 #Duplication Level Percentage of deduplicated Percentage of total 1 86.66773908147671 65.21218136483576 2 8.03667658650366 12.094216756638989 3 2.50784203329848 5.660993972257861 4 1.0761682618836965 3.239003606962163 5 0.543714308694603 2.0455590791352973 6 0.31850719858441245 1.4379433053329782 7 0.20429502131424435 1.0760366990244257 8 0.11742942170031367 0.706867752789949 9 0.09973457733451299 0.6753976131109444 >10 0.4069814204134159 4.594640393134668 >50 0.014477599935655111 0.6959742429010627 >100 0.004825866645218371 1.1159795686170084 >500 0.0 0.0 >1k 0.0016086222150727902 1.4452056452589024 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1194 1.4452056452589024 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 348 0.4212157157035997 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 307 0.3715897262097848 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 267 0.32317412670362394 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.007262339925924133 0.0 0.0 0.0 0.0 11 0.012103899876540222 0.0 0.0 0.0 0.0 12 0.014524679851848266 0.0 0.0 0.0 0.0 13 0.016945459827156308 0.0 0.0 0.0 0.0 14 0.020576629790118376 0.0 0.0 0.0 0.0 15 0.027838969716042508 0.0 0.0 0.0 0.0 16 0.036311699629620664 0.0 0.0 0.0 0.0 17 0.04236364956789077 0.0 0.0 0.0 0.0 18 0.05204676946912295 0.0 0.0 0.0 0.0 19 0.05688832941973904 0.0 0.0 0.0 0.0 20 0.06657144932097121 0.0 0.0 0.0 0.0 21 0.07262339925924133 0.0 0.0 0.0 0.0 22 0.0823065191604735 0.0 0.0 0.0 0.0 23 0.09198963906170568 0.0 0.0 0.0 0.0 24 0.09925197898762982 0.0 0.0 0.0 0.0 25 0.11377665883947807 0.0 0.0 0.0 0.0 26 0.12467016872836428 0.0 0.0 0.0 0.0 27 0.13314289864194243 0.0 0.0 0.0 0.0 28 0.15129874845675276 0.0 0.0 0.0 0.0 29 0.15492991841971482 0.0 0.0 0.0 0.0 30 0.16098186835798495 0.0 0.0 0.0 0.0 31 0.16703381829625505 0.0 0.0 0.0 0.0 32 0.17187537824687113 0.0 0.0 0.0 0.0 33 0.17550654820983322 0.0 0.0 0.0 0.0 34 0.17550654820983322 0.0 0.0 0.0 0.0 35 0.17671693819748724 0.0 0.0 0.0 0.0 36 0.17792732818514126 0.0 0.0 0.0 0.0 37 0.1803481081604493 0.0 0.0 0.0 0.0 38 0.18276888813575734 0.0 0.0 0.0 0.0 39 0.18518966811106538 0.0 0.0 0.0 0.0 40 0.18518966811106538 0.0 0.0 0.0 0.0 41 0.18882083807402744 0.0 0.0 0.0 0.0 42 0.1972935679876056 0.0 0.0 0.0 0.0 43 0.20455590791352973 0.0 0.0 0.0 0.0 44 0.21060785785179986 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCC 15 0.007988544 50.643826 38 CCTTAAG 15 0.0080276355 50.581383 41 TAAGGTA 15 0.008205307 50.30227 44 CCCATTC 15 0.008205307 50.30227 43 CGATTAA 50 7.2759576E-12 50.271446 49 CGATTCA 20 4.0512258E-4 50.271442 49 TTCTAGC 15 0.008245183 50.24066 34 GGTAATG 15 0.008245183 50.24066 47 ACGATTC 25 2.012346E-5 50.24066 48 ACGATTA 50 7.2759576E-12 50.24066 48 TCACTTG 15 0.008245183 50.24066 34 ATTGCAT 15 0.008245183 50.24066 46 TACAGCA 25 2.012346E-5 50.24066 47 CTGTGAT 15 0.008245183 50.24066 47 CTTAAGG 15 0.008265176 50.209915 42 CAGGAAA 15 0.0084467335 49.934875 32 GCATGTG 15 0.0084467335 49.934875 32 CTTAAAC 15 0.0084670875 49.9045 13 CTCCCAT 15 0.0084670875 49.9045 26 CGGGCGT 15 0.0084670875 49.9045 30 >>END_MODULE