FastQCFastQC Report
Fri 17 Jun 2016
SRR1526262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49574
Sequences flagged as poor quality0
Sequence length56
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2700.5446403356598217No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG2380.48009036995199095No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG2020.4074716585306814No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1730.3489732521079598No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1180.23802799854762577No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG980.1976842699802316No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG900.1815467785532739No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG880.17751240569653445No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC870.17549521926816478No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG780.15734054141283738No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC720.14523742284261912No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG680.13716867712914027No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG670.13515149070077057No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC660.13313430427240086No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG560.11296243998870376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTACT203.975758E-450.42288239
ACTTCCT150.00810870250.42288240
TACGCCC402.406523E-950.42288238
TTTACTT203.975758E-450.42288240
GCTTGTC251.958028E-550.4228838
GCCCAGT354.859612E-850.3714341
TGTCTCA251.9699191E-550.3714341
CCACAGT451.2187229E-1050.3714341
TTACTTT203.9958768E-450.37142641
ATATGCT354.9385562E-850.26883734
CAGCAAA150.00824064450.217747
TTCGCGC150.00824064450.217749
CTTTGAG150.00824064450.217749
CTTCCGA150.00824064450.217746
GATAGAA150.00830721150.11573843
CTTACTG150.00834064550.06490750
CTCTCTA150.00837417850.01418732
CATATGC402.586603E-950.01418333
TGTCCCG150.00840781249.96356215
CCAGGAC150.00840781249.96356216