##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526262_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 49574 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.29465042159196 38.0 37.0 39.0 33.0 40.0 2 36.59111631096946 38.0 36.0 39.0 31.0 40.0 3 35.661959898333805 38.0 35.0 39.0 29.0 40.0 4 36.27036349699439 38.0 35.0 39.0 30.0 40.0 5 34.813551458425785 37.0 33.0 39.0 26.0 40.0 6 34.900774599588495 38.0 35.0 40.0 18.0 40.0 7 36.920704401500785 38.0 36.0 39.0 32.0 40.0 8 36.887743575261226 38.0 36.0 40.0 32.0 40.0 9 36.857344575785696 38.0 37.0 40.0 31.0 40.0 10 36.219974180013715 38.0 35.0 39.0 30.0 40.0 11 35.7874288941784 38.0 35.0 39.0 29.0 40.0 12 33.775345947472466 38.0 32.0 39.0 21.0 40.0 13 33.519627223948035 38.0 31.0 39.0 20.0 40.0 14 33.65124863839916 38.0 31.0 39.0 20.0 40.0 15 33.7154153386856 38.0 32.0 39.0 20.0 40.0 16 33.86908460079881 38.0 32.0 39.0 22.0 40.0 17 33.94390204542704 38.0 32.0 39.0 22.0 40.0 18 33.38834873118974 38.0 31.0 39.0 19.0 40.0 19 34.28071166337193 38.0 32.0 39.0 24.0 40.0 20 34.32730866986727 38.0 32.0 39.0 24.0 40.0 21 35.11070319118893 38.0 34.0 39.0 26.0 40.0 22 34.81754548755396 38.0 33.0 39.0 25.0 40.0 23 35.25551700488159 38.0 34.0 39.0 27.0 40.0 24 34.78180094404325 38.0 33.0 39.0 25.0 40.0 25 35.11124783152459 38.0 34.0 39.0 27.0 40.0 26 35.59448501230484 38.0 35.0 39.0 28.0 40.0 27 35.53429216928229 38.0 35.0 39.0 28.0 40.0 28 35.42132972929358 38.0 35.0 39.0 28.0 40.0 29 35.283475208778796 38.0 35.0 39.0 27.0 40.0 30 35.05016742647356 38.0 35.0 39.0 27.0 40.0 31 35.48729172550127 38.0 35.0 39.0 29.0 40.0 32 35.07760116189938 38.0 35.0 39.0 27.0 40.0 33 35.26790252955178 38.0 35.0 39.0 28.0 40.0 34 35.161072336305324 38.0 35.0 39.0 28.0 40.0 35 35.12982611852988 38.0 35.0 39.0 28.0 40.0 36 34.47083148424577 38.0 33.0 39.0 28.0 40.0 37 34.23679751482632 37.0 33.0 39.0 27.0 40.0 38 33.72245531932061 36.0 33.0 39.0 26.0 40.0 39 33.565982168071976 36.0 32.0 39.0 24.0 40.0 40 30.198188566587323 35.0 26.0 38.0 10.0 40.0 41 26.81181667809739 32.0 16.0 38.0 6.0 39.0 42 30.96094727074676 35.0 29.0 38.0 12.0 39.0 43 29.534735950296525 34.0 27.0 38.0 6.0 39.0 44 29.502965264049703 34.0 27.0 38.0 5.0 39.0 45 30.68735627546698 35.0 29.0 38.0 4.0 39.0 46 30.351071125993464 35.0 28.0 38.0 4.0 40.0 47 29.8474401904224 35.0 27.0 38.0 4.0 40.0 48 30.02144269173357 35.0 27.0 38.0 4.0 40.0 49 27.425787711300277 33.0 17.0 38.0 4.0 40.0 50 29.622342356880623 35.0 27.0 38.0 4.0 40.0 51 29.798805825634407 35.0 28.0 39.0 4.0 40.0 52 29.51149796264171 35.0 27.0 39.0 4.0 40.0 53 29.106910880703595 35.0 26.0 39.0 4.0 40.0 54 28.070097228385848 34.0 21.0 39.0 4.0 40.0 55 28.506555855892202 35.0 24.0 39.0 4.0 40.0 56 27.59246782587647 34.0 18.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.2040395606271659 1 2 1.7568474453684502 1 3 2.977927389378536 1 4 2.0156211648943767 1 5 3.2747600205554193 1 6 3.9602384538137727 1 7 1.4968970084646074 1 8 1.2250780153489416 1 9 1.5075275791391576 1 10 2.1458733573110607 1 11 -1.3898792765171777 1 12 -11.21103883490354 1 13 -8.486867075657926 1 14 -4.442355045768814 1 15 -5.452586219368946 1 16 -23.68669240601085 1 17 -3.8301104308487126 1 18 -12.148773639113493 1 19 -5.940513889105354 1 20 -5.824779469739838 1 21 -6.415063022893136 1 22 -2.8819434700137307 1 23 -2.1483925539783755 1 24 -2.845875778071459 1 25 -2.1960106428316877 1 26 2.4297999247369546 1 27 2.526961844854654 1 28 1.9104941784177711 1 29 2.551087788551044 1 30 2.5285470905752163 1 31 0.710880071883004 1 32 1.9492529898090396 1 33 0.8633315634672201 1 34 0.8916368659055678 1 35 1.3987117175130308 1 36 1.85876232052955 1 37 2.2618596650799887 1 38 1.1927478505269207 1 39 1.8468122202927972 1 40 4.433317290779147 1 41 -10.51215565939416 1 42 -10.06365548499603 1 43 -18.492310403109574 1 44 -21.71721597173242 1 45 -12.756718992021813 1 46 -17.85998206634272 1 47 -23.775605130954325 1 48 -15.693755399704521 1 49 -20.702335204181324 1 50 -14.162891426804926 1 51 -10.66768397580853 1 52 -10.709109791704716 1 53 -10.8568896710485 1 54 -10.131409030152607 1 55 -10.774538032533357 1 56 -5.822872364616341 2 1 1.4058441380919575 2 2 1.8516977974811297 2 3 2.955480206279944 2 4 2.360691587429585 2 5 3.0260804430906276 2 6 4.662263101701093 2 7 1.9847931352251678 2 8 1.8564600576024617 2 9 1.890450114350422 2 10 2.4566127939307805 2 11 1.9208601601025421 2 12 -2.8411004546218486 2 13 -0.5632315122776461 2 14 3.1102417852170987 2 15 4.153267653870493 2 16 2.867664988355351 2 17 2.6846342874611437 2 18 -1.6960887799585649 2 19 2.2571093503312625 2 20 2.2619282767390345 2 21 2.2889422587970074 2 22 2.1352220229440135 2 23 1.5906039248948645 2 24 1.8675925317876931 2 25 2.5896407656190163 2 26 1.9372823191031543 2 27 3.068775225136342 2 28 2.587430798136083 2 29 3.299107154748228 2 30 2.34192737226536 2 31 0.5035737338548358 2 32 0.4334079193865037 2 33 0.7953298029038365 2 34 1.0128956687224715 2 35 0.8742839006116228 2 36 1.1776443627830702 2 37 0.3806976932490045 2 38 1.4293235547522727 2 39 1.4988808822646291 2 40 4.318220459793235 2 41 -6.567613405873033 2 42 -6.22364668217913 2 43 -14.481526952405348 2 44 -14.648774070323968 2 45 -6.531586949768293 2 46 -11.115703897328636 2 47 -18.972348088700805 2 48 -9.666466667310154 2 49 -13.516595767561606 2 50 -9.276447764833094 2 51 -5.194972708202897 2 52 -4.908273524099084 2 53 -4.642321009076667 2 54 -3.482201283673735 2 55 -4.039502821265753 2 56 -1.463497364616341 3 1 2.4036923384049445 3 2 3.1752502231462287 3 3 4.127232944934093 3 4 3.7795100537832624 3 5 4.625454464999862 3 6 5.588710676035994 3 7 2.9673831195757217 3 8 2.7858419042378273 3 9 3.0144720235836004 3 10 3.013928912866618 3 11 3.950398501260601 3 12 6.2177806095409025 3 13 5.393341257675409 3 14 4.823269954231186 3 15 5.059566558408832 3 16 5.5650437051002655 3 17 5.289681235817952 3 18 5.918934694219843 3 19 5.01434722200576 3 20 4.852303863593498 3 21 3.9894508659957495 3 22 4.454862085541826 3 23 3.855079668243846 3 24 4.400651999706319 3 25 4.133850468279427 3 26 2.7179943691813975 3 27 2.933211844854654 3 28 2.6396608450844354 3 29 3.3444905663288225 3 30 3.167435979464102 3 31 2.554630071883004 3 32 3.421475212031261 3 33 2.760901007911663 3 34 1.643372977016682 3 35 2.563642273068588 3 36 3.898692876085107 3 37 3.3521374428577673 3 38 3.131983961638035 3 39 4.452714998070576 3 40 7.688525624112483 3 41 9.369788785050282 3 42 5.9623861816706345 3 43 1.1726201524459832 3 44 3.7984090282675815 3 45 5.342239341311522 3 46 4.782379044768394 3 47 -1.0012995753987681 3 48 5.257633489184364 3 49 7.054609240263119 3 50 4.60794190652841 3 51 6.617038246413692 3 52 6.556515208295284 3 53 7.2976242178403865 3 54 7.441507636514057 3 55 6.8435175230222 3 56 6.175391524272545 4 1 1.9610594881634 4 2 2.4228347642090284 4 3 3.3071846357553483 4 4 3.0663458025755332 4 5 3.9491259625844037 4 6 5.467937729176093 4 7 2.5863444722327245 4 8 2.7719530153489416 4 9 2.834520332762345 4 10 3.3804748065864203 4 11 3.5113888394248534 4 12 6.122973846255881 4 13 5.643341257675409 4 14 5.593197490463069 4 15 5.827078635703515 4 16 5.5137152026848 4 17 5.871202974948389 4 18 5.962412955089405 4 19 5.126062197851169 4 20 5.073318356347116 4 21 3.711069223483676 4 22 4.091335515493519 4 23 3.647350199644812 4 24 4.233381468305353 4 25 3.88868138615382 4 26 3.0036223885050717 4 27 3.5400959028256693 4 28 2.9132115697221224 4 29 3.0884519189858253 4 30 3.546663032604201 4 31 2.498470651593152 4 32 2.9075863231423753 4 33 1.8381956939020014 4 34 2.1318995470649895 4 35 1.928376572585492 4 36 3.281543117631003 4 37 3.7591422737756375 4 38 2.977394589657358 4 39 4.16044446666961 4 40 7.835264754547264 4 41 9.620996514518886 4 42 5.11274850051122 4 43 7.298224017180278 4 44 6.751307578992222 4 45 6.586804558702827 4 46 5.560760687280467 4 47 4.638797042958721 4 48 4.347005469860697 4 49 6.957990882775199 4 50 5.457579587687825 4 51 4.313294285061033 4 52 4.628978976411229 4 53 4.598952720255852 4 54 5.633536622021307 4 55 3.624918489205772 4 56 4.391575099151776 5 1 1.9410554056975897 5 2 2.4009935721290105 5 3 2.687874572477128 5 4 2.811395812781697 5 5 3.378193119146971 5 6 5.0143757777574365 5 7 2.4073283464927755 5 8 2.335333297039085 5 9 2.5101684242095814 5 10 3.118584624916693 5 11 3.611001005172966 5 12 5.6800263059415315 5 13 5.66212060039841 5 14 5.3919319260621705 5 15 5.660309907391614 5 16 5.0648480869468955 5 17 4.360690625489319 5 18 5.557432346802003 5 19 4.46837695596507 5 20 4.529533910541851 5 21 3.767815498233624 5 22 3.8253628680144374 5 23 3.435674347430073 5 24 3.825339010660933 5 25 3.4487956951964804 5 26 2.500662600793291 5 27 3.111028746263102 5 28 2.263487136164251 5 29 3.101924056156676 5 30 2.116575259589304 5 31 2.771179367657652 5 32 2.4052389053019994 5 33 2.0347664226221482 5 34 2.477684401116832 5 35 2.3954106611749992 5 36 2.684686616304198 5 37 2.901824453812381 5 38 2.415239047710017 5 39 4.090430178039277 5 40 6.5999106006382995 5 41 10.192949974408656 5 42 6.283395571342002 5 43 7.208613892665074 5 44 7.30897240854927 5 45 5.721934176992274 5 46 5.58852145478404 5 47 5.971313883130179 5 48 5.249026290436326 5 49 8.089038035255292 5 50 5.146087446434514 5 51 5.466999122783022 5 52 5.1762335181544366 5 53 4.850636737402205 5 54 5.9966719557628885 5 55 4.256271826621571 5 56 4.606925170594927 6 1 1.883267501803637 6 2 2.162178327721392 6 3 2.321677389378536 6 4 2.0450329296002607 6 5 3.9457159029083613 6 6 5.116488453813773 6 7 1.8636249496410784 6 8 1.5807765447607096 6 9 1.4817922850215126 6 10 1.7304321808404737 6 11 2.9152677823063513 6 12 5.133630282743518 6 13 4.265890277283248 6 14 5.580622895407657 6 15 5.583259368866351 6 16 3.681873770459738 6 17 4.953896922092461 6 18 4.762071949121804 6 19 4.440000816777001 6 20 4.474852883201336 6 21 2.284385506518632 6 22 3.0463653535156823 6 23 1.8534456813157405 6 24 3.497874221928541 6 25 2.9933275924624283 6 26 1.7367852188545996 6 27 2.1317412566193568 6 28 1.8774059431236552 6 29 2.184359847374573 6 30 3.0432529729281583 6 31 0.988453601294772 6 32 1.1771941662796266 6 33 1.434103622290749 6 34 1.3043206894349808 6 35 1.3610278939836178 6 36 1.357843202882492 6 37 2.0008302533152857 6 38 2.3738140269975077 6 39 1.8808195732339712 6 40 5.12725111430856 6 41 3.116520811194075 6 42 4.185425397356912 6 43 4.925888126302194 6 44 3.504291381208759 6 45 3.9280236550370127 6 46 4.413914992480809 6 47 5.10490957492803 6 48 4.706979894413124 6 49 6.830753031112792 6 50 4.384902690842132 6 51 4.593345435956174 6 52 4.938868149471755 6 53 4.989617681892675 6 54 4.73072332278857 6 55 4.758550202760759 6 56 4.742384988324837 7 1 1.8915395606271659 7 2 2.49122244536845 7 3 3.102927389378536 7 4 2.6874961648943767 7 5 3.4153850205554193 7 6 5.022738453813773 7 7 2.4656470084646074 7 8 2.1938280153489416 7 9 2.2887775791391576 7 10 2.5521233573110607 7 11 3.0476207234828223 7 12 5.57021116509646 7 13 5.497507924342074 7 14 4.448269954231186 7 15 5.469288780631054 7 16 5.032057593989151 7 17 5.201139569151287 7 18 5.101226360886507 7 19 3.887611110894646 7 20 4.518970530260162 7 21 3.5224369771068638 7 22 3.9149315299862693 7 23 3.3672324460216245 7 24 3.560374221928541 7 25 2.9289893571683123 7 26 2.2579249247369546 7 27 2.339461844854654 7 28 2.926119178417771 7 29 2.519837788551044 7 30 1.6066720905752163 7 31 0.523380071883004 7 32 0.9805029898090396 7 33 0.5195815634672201 7 34 0.6260118659055678 7 35 0.1487117175130308 7 36 0.98376232052955 7 37 1.1056096650799887 7 38 1.0364978505269207 7 39 2.1436872202927972 7 40 4.417692290779147 7 41 0.4878443406058395 7 42 3.54571951500397 7 43 4.492064596890426 7 44 4.7046590282675815 7 45 3.1807810079781866 7 46 4.31189293365728 7 47 4.286894869045675 7 48 4.587494600295479 7 49 6.844539795818676 7 50 3.8683585731950743 7 51 4.238566024191471 7 52 4.728390208295284 7 53 3.3618603289515008 7 54 5.009215969847393 7 55 2.772336967466643 7 56 4.755252635383659 8 1 2.0921844018970077 8 2 2.8776311755271777 8 3 3.242312310013453 8 4 3.1981608474340604 8 5 4.196883036428439 8 6 5.318869406194722 8 7 2.715398992591588 8 8 2.6132228566187834 8 9 2.740662499774075 8 10 2.8373416112793137 8 11 3.571430247292348 8 12 5.747542514302808 8 13 5.119531733865884 8 14 5.702238208199439 8 15 5.851233225075497 8 16 5.237662752719309 8 17 4.32737964851637 8 18 5.7602045354896845 8 19 4.27029960295814 8 20 4.641986403276036 8 21 3.8525461040909903 8 22 4.308036688716427 8 23 3.7757145888787704 8 24 4.295493269547592 8 25 3.5346441190730715 8 26 2.7517245279115556 8 27 2.879640416283223 8 28 3.046406876830467 8 29 2.376236598074854 8 30 3.7785470905752163 8 31 2.4970903893433203 8 32 2.4631418786979253 8 33 1.713281960292619 8 34 2.359642818286517 8 35 1.571578781005094 8 36 1.489962717354949 8 37 1.0227723634926846 8 38 1.941507771161838 8 39 2.25430229965788 8 40 4.3452716558585145 8 41 -2.598465183203686 8 42 2.7262750705595273 8 43 4.807540787366616 8 44 4.849996329854886 8 45 1.8918425159146963 8 46 3.0383314257207736 8 47 3.768541694442497 8 48 3.344935076485953 8 49 7.118101303755182 8 50 3.986910160496663 8 51 5.523784278159724 8 52 4.9692136209936955 8 53 4.652782947999121 8 54 4.469285414291836 8 55 5.2681206976253705 8 56 4.822216921097944 9 1 1.9928500444981339 9 2 2.64747244536845 9 3 3.4507096474430554 9 4 3.128524390700825 9 5 4.100364859265099 9 6 5.205198131233125 9 7 2.8119171697549277 9 8 2.642920757284422 9 9 2.690995321074638 9 10 3.3091798089239646 9 11 3.966975562192502 9 12 6.011239390902908 9 13 5.680975666277554 9 14 6.2385925348763465 9 15 5.26162749030847 9 16 5.627319690763343 9 17 5.554466182054512 9 18 5.327536844757475 9 19 4.665332885088198 9 20 5.091551175421451 9 21 3.983626493235896 9 22 4.794467820308853 9 23 3.8425348653764644 9 24 4.731745189670477 9 25 4.30499742168444 9 26 3.745324118285339 9 27 3.4372441029191734 9 28 3.541542565514547 9 29 3.615099885325236 9 30 3.8269341873494085 9 31 3.5667268460765555 9 32 3.0097368607767834 9 33 2.3971017247575404 9 34 3.6330683175184717 9 35 3.266151233642063 9 36 3.4802340947231016 9 37 4.034037084434829 9 38 4.456356721494664 9 39 4.707195284808925 9 40 6.205998742392051 9 41 3.7963120825413235 9 42 5.928784031133002 9 43 7.084806532374298 9 44 7.08066709278371 9 45 5.561325362816898 9 46 7.25594535301212 9 47 7.3786287400134185 9 48 6.75584137448903 9 49 9.400487376463836 9 50 6.625415024807978 9 51 6.802074088707599 9 52 5.814579724424316 9 53 6.291799845080533 9 54 6.589357098879649 9 55 6.570220031982771 9 56 6.197792957964307 10 1 1.7665395606271659 10 2 1.9912224453684502 10 3 2.087302389378536 10 4 2.8437461648943767 10 5 3.5247600205554193 10 6 4.725863453813773 10 7 1.7000220084646074 10 8 1.7563280153489416 10 9 1.5544025791391576 10 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3.5061807987904174 115 37 1.9982727085582468 115 38 3.9121771983530067 115 39 4.566241568118883 115 40 7.0019314212139285 115 41 6.794909557997144 115 42 1.0837629932648412 115 43 -6.16554409876175 115 44 -8.777677928254157 115 45 1.2969494862390576 115 46 -3.931992935907939 115 47 -3.817724696171716 115 48 -4.131255399704521 115 49 -14.737661291137846 115 50 -3.8612609920223164 115 51 -2.3171404975476584 115 52 -2.9217456612699344 115 53 -3.270612497135456 115 54 -7.236028595369998 115 55 -1.6359510760116187 115 56 0.7973722006010497 116 1 1.41455092426353 116 2 2.2929269908229983 116 3 3.158041025742172 116 4 2.442893892167106 116 5 3.581010020555418 116 6 4.475579362904682 116 7 1.803999281191878 116 8 1.7537711971671257 116 9 1.8018457609573417 116 10 2.401555175492881 116 11 -0.49783382197172443 116 12 -10.583482016721721 116 13 -7.489992075657927 116 14 -2.3153664094051756 116 15 -6.411393037550763 116 16 -2.121067406010848 116 17 -2.2974399763032594 116 18 -7.0143986391134945 116 19 -3.3672184345598986 116 20 -2.8128476515580196 116 21 2.7801074316523184 116 22 3.367488348168088 116 23 2.6328574460216245 116 24 3.1024196764739926 116 25 2.863648448077406 116 26 2.930368106555136 116 27 2.8173027539455617 116 28 1.9116305420541337 116 29 2.530349152187405 116 30 3.142183454211583 116 31 2.306902799155729 116 32 3.010616626172677 116 33 2.5346383816490388 116 34 1.7890800477237505 116 35 2.0899048993312093 116 36 3.238591865984098 116 37 2.7243596650799873 116 38 3.0208728505269207 116 39 3.356755402110977 116 40 7.9256468362336925 116 41 4.107162522424023 116 42 -0.702007757723301 116 43 -6.7829354031095725 116 44 -6.469772789914238 116 45 -0.07063944656726662 116 46 -3.54464115725181 116 47 -1.4204914945906886 116 48 -3.8948917633408833 116 49 -7.13528974963587 116 50 -3.218573244986743 116 51 -1.5120021576267106 116 52 -1.5525757007956251 116 53 -3.2935373983212273 116 54 -7.302431757425335 116 55 -0.6256743961697211 116 56 -0.372588273707251 117 1 0.8509145606271673 117 2 1.472472445368453 117 3 2.2966773893785373 117 4 1.3906211648943767 117 5 2.6060100205554164 117 6 3.716488453813774 117 7 1.1812720084646102 117 8 1.0969530153489444 117 9 1.3450275791391562 117 10 1.7833733573110635 117 11 -1.2773792765171805 117 12 -9.85166383490354 117 13 -11.539992075657928 117 14 -7.951730045768812 117 15 -7.018211219368947 117 16 -13.671067406010849 117 17 -11.276985430848711 117 18 -13.314398639113492 117 19 -5.4967638891053525 117 20 -6.481029469739838 117 21 0.4005619771068609 117 22 0.8493065299862721 117 23 0.35785744602162595 117 24 -0.22712577807146062 117 25 -0.7272606428316877 117 26 0.6485499247369546 117 27 0.9582118448546524 117 28 1.8729941784177697 117 29 1.2167127885510425 117 30 1.6285470905752177 117 31 1.1546300718830054 117 32 1.6242529898090368 117 33 1.56645656346722 117 34 1.1072618659055706 117 35 1.558086717513028 117 36 1.8681373205295486 117 37 1.8743596650799859 117 38 2.5208728505269207 117 39 2.824937220292796 117 40 6.255192290779146 117 41 2.950344340605838 117 42 -1.6792804849960312 117 43 -10.057935403109575 117 44 -14.176590971732418 117 45 -2.266093992021812 117 46 -5.878732066342721 117 47 -6.431855130954325 117 48 -5.606255399704523 117 49 -11.239835204181324 117 50 -5.675391426804925 117 51 -6.480183975808529 117 52 -6.2434847917047165 117 53 -7.8912646710485 117 54 -9.875159030152606 117 55 -5.6870380325333585 117 56 -4.188497364616342 118 1 1.1145509242635327 118 2 1.8065633544593567 118 3 2.4807682984694424 118 4 2.3110757103489235 118 5 2.862828202373599 118 6 4.139215726541046 118 7 0.6630901902827873 118 8 1.6355893789853084 118 9 1.6836639427755244 118 10 2.106100630038334 118 11 -2.088742912880811 118 12 -7.669845653085357 118 13 -7.508173893839743 118 14 -10.59718459122336 118 15 -5.86593849209622 118 16 -14.602885587829032 118 17 -11.842894521757803 118 18 -11.98258045729531 118 19 -5.699036616378081 118 20 -5.185574924285291 118 21 0.8619256134704969 118 22 0.944761075440816 118 23 1.223766536930718 118 24 0.7706014946558142 118 25 0.8409211753501324 118 26 0.8076408338278611 118 27 1.6718482084910207 118 28 1.529812360235951 118 29 1.134894606732864 118 30 1.8694561814843098 118 31 0.8387209809739105 118 32 -3.903019737463687 118 33 1.3619111089217668 118 34 1.0890800477237477 118 35 1.0535412629675776 118 36 1.52495550234773 118 37 1.3016323923527153 118 38 0.7254183050723739 118 39 2.0613008566564304 118 40 5.775646836233694 118 41 1.607162522424023 118 42 -5.306553212268756 118 43 -11.51929903947321 118 44 -10.837954608096055 118 45 -4.00245762838545 118 46 -9.79464115725181 118 47 -11.352309676408872 118 48 -10.858528126977248 118 49 -11.61710793145405 118 50 -8.986755063168562 118 51 -5.139274884899439 118 52 -4.625302973522899 118 53 -4.693537398321226 118 54 -11.566068121061699 118 55 -6.030219850715174 118 56 -3.713497364616341 119 1 0.6655487069686288 119 2 0.8547895185391852 119 3 1.7363115357199987 119 4 0.8083040917236417 119 5 2.2054002644578574 119 6 3.0250250391796243 119 7 0.17029639870850843 119 8 0.3817091129099168 119 9 0.5029544084074473 119 10 1.0223977475549617 119 11 -4.562135374078153 119 12 -13.065078469049883 119 13 -12.64121158785305 119 14 -13.673681265281008 119 15 -7.788333170588459 119 16 -15.750335698693775 119 17 -14.239790308897494 119 18 -16.7314718098452 119 19 -7.075422425690721 119 20 -9.231029469739838 119 21 -0.5671209497224012 119 22 1.2224772616935837 119 23 0.5352964704118719 119 24 1.3698254414407387 119 25 0.16298325960733706 119 26 1.4656230954686649 119 27 1.332602088757092 119 28 1.323603934515333 119 29 2.1032981544047047 119 30 1.022449529599605 119 31 0.10645934017568948 119 32 -5.181844571166572 119 33 0.2920663195647819 119 34 0.7731155244421544 119 35 0.532476961415469 119 36 1.385820247358815 119 37 1.5682621041043774 119 38 1.6062387041854578 119 39 3.028595756878161 119 40 5.248484973705974 119 41 1.779002877191207 119 42 -3.365865850849687 119 43 -9.919520768963231 119 44 -9.915005605878761 119 45 -4.9819476505584 119 46 -15.063488163903695 119 47 -13.325147813881154 119 48 -12.69223100946062 119 49 -11.70690837491303 119 50 -9.332098743878095 119 51 -7.21798885385731 119 52 -6.736167718533984 119 53 -8.746752475926549 119 54 -11.438573664298948 119 55 -6.974233154484576 119 56 -4.122033949982196 120 1 -0.20069834259864194 120 2 0.6313434131103861 120 3 1.9668386797011195 120 4 1.128524390700825 120 5 2.2455261495876755 120 6 2.947133615104093 120 7 0.11836878265815898 120 8 0.38485624115538997 120 9 0.5297049984939974 120 10 1.0995023895691247 120 11 -2.9523792765171777 120 12 -14.472631576839024 120 13 -8.657734011141798 120 14 -17.390439723188166 120 15 -12.141598316143138 120 16 -18.437196438268913 120 17 -14.397146721171293 120 18 -17.220850252016717 120 19 -7.931441308460194 120 20 -14.360061727804354 120 21 -4.306696087409264 120 22 -4.173274115175023 120 23 -2.8187554572041833 120 24 -9.074706423232751 120 25 -2.904679997670396 120 26 1.6001628279627624 120 27 -0.1111429938550259 120 28 0.799607081643579 120 29 0.7925192401639478 120 30 0.9720954776719921 120 31 0.17963007188300395 120 32 -11.328972816642572 120 33 0.3487146279833482 120 34 0.11693928526040764 120 35 0.5080867175130308 120 36 1.7382986108521337 120 37 1.937262890886437 120 38 1.891840592462401 120 39 2.5297759299702136 120 40 4.625353581101731 120 41 2.5059895018961633 120 42 -1.861538549512158 120 43 -9.834548306335382 120 44 -10.77417161689371 120 45 -6.261255282344393 120 46 -16.582764324407236 120 47 -16.314919647083357 120 48 -11.905448948091617 120 49 -18.776931978374872 120 50 -12.084262394546862 120 51 -9.891474298389173 120 52 -10.443484791704716 120 53 -10.804974348467855 120 54 -14.620320320475187 120 55 -9.687844484146261 120 56 -7.173174783971181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 366.0 5 17.0 6 48.0 7 58.0 8 55.0 9 40.0 10 33.0 11 48.0 12 48.0 13 64.0 14 70.0 15 121.0 16 164.0 17 193.0 18 211.0 19 309.0 20 363.0 21 513.0 22 666.0 23 714.0 24 872.0 25 962.0 26 1030.0 27 1173.0 28 1362.0 29 1577.0 30 1849.0 31 2174.0 32 2406.0 33 2882.0 34 3400.0 35 4042.0 36 5059.0 37 6294.0 38 6404.0 39 3985.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.3666034614919 0.4054544721023117 0.1855811514100133 0.04236091499576391 8 99.53604712147497 0.37721386210513574 0.05244684713761246 0.03429216928228507 9 98.50929922943479 0.8189776899181023 0.2803889135433897 0.3913341671037237 10 45.053858877637474 39.236293218219224 8.385443982732884 7.324403921410417 11 29.40452656634526 29.933029410578126 25.676766046718036 14.985677976358575 12 41.71339815225723 14.342195505708638 19.732117642312502 24.21228869972163 13 22.194649558164873 20.955897187588267 24.06286567405076 32.786587580196105 14 27.96280233191455 18.7884533919674 28.87660621709398 24.372138059024063 15 26.477842471292252 22.210092188258127 25.141517062914442 26.170548277535175 16 30.273859256119955 18.278137676333476 30.350547920324516 21.097455147222057 17 31.954250211804574 22.431113083471175 23.21781579053536 22.39682091418889 18 32.375131122407815 21.124828532235938 23.551601710643105 22.948438634713145 19 25.883445741286703 28.803360122773718 22.08109527078874 23.232098865150842 20 26.129182062309447 25.695075413410866 19.912371029943262 28.26337149433642 21 27.29253237584218 22.11038044136039 28.48872392786541 22.10836325493202 22 26.223423568806233 22.985839351272844 25.311655303183123 25.479081776737804 23 29.96933876628878 26.86690603945617 21.533465122846653 21.6302900714084 24 26.382781296647433 28.026788235768752 24.601605680396982 20.988824787186832 25 27.73833057651188 27.90979142292331 22.552144269173358 21.799733731391456 26 27.29455440550411 21.413150939208684 27.982567641183948 23.309727014103263 27 28.770165768166862 20.13245300543138 27.284107658448924 23.813273567952834 28 24.245239201114725 27.02800945091783 27.532866172580224 21.193885175387226 29 25.119573772476844 26.043873988415978 29.024641278682566 19.811910960424616 30 22.36682808716707 19.814366424535915 38.052865213882164 19.765940274414852 31 22.600694164177902 20.374929372830735 38.07208007103075 18.95229639196061 32 29.18408141013992 19.64786883921901 33.19603448623983 17.972015264401236 33 22.785321694148074 23.91084707403815 34.18606530876172 19.11776592305205 34 19.6689877888788 21.576344737107682 38.207689978807146 20.546977495206377 35 24.273431823686124 20.674901105998224 39.75337046904012 15.29829660127553 36 24.61606764474401 22.49510624987387 36.80705507234678 16.081771033035334 37 25.370811946813237 21.177706826173058 38.231014832477875 15.220466394535828 38 21.231796116504853 22.841828478964402 40.96076051779936 14.965614886731391 39 20.721157733042283 25.248605384428814 36.3165979464791 17.713638936049804 40 15.68595578673602 16.043563068920676 33.62118010403121 34.64930104031209 41 16.572044848706835 15.422338888549742 32.37897564251267 35.62664062023075 42 18.356092436974787 21.456851971557853 37.25759534583064 22.929460245636715 43 15.814799838253135 21.09381318236959 38.782854832187624 24.30853214718965 44 16.422751043904814 17.387602870231483 36.67653139822435 29.51311468763936 45 18.457651101677786 24.47543966040024 39.14695775217304 17.91995148574894 46 21.944416371905 20.446690247599797 38.33249115715008 19.276402223345123 47 16.815998708479636 20.995277878677808 43.201356096379705 18.98736731646285 48 18.342190878412513 22.01992442459636 42.26362479034898 17.37425990664215 49 15.52492906364005 18.471828131333602 33.717065261451154 32.28617754357519 50 19.02058627447019 21.036788621992365 43.3140063435625 16.62861875997495 51 22.979481160710673 22.414909547128577 38.2654093650087 16.34019992715205 52 17.687335891739043 26.014946475459507 39.268834578872955 17.0288830539285 53 21.84683774499899 21.877146898363307 39.4887856132552 16.7872297433825 54 16.11324570273003 22.084934277047523 39.854398382204245 21.9474216380182 55 17.956599995955266 23.17026310999656 37.05179282868526 21.821344065362915 56 23.812315529858893 22.296122589253226 35.852909068855375 18.038652812032506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 17.5 11 34.0 12 69.0 13 115.5 14 127.0 15 150.5 16 174.0 17 203.5 18 233.0 19 296.5 20 360.0 21 395.5 22 503.0 23 575.0 24 757.0 25 939.0 26 1143.0 27 1347.0 28 1565.5 29 1914.5 30 2045.0 31 2065.0 32 2085.0 33 1967.5 34 1850.0 35 1602.0 36 1354.0 37 1238.5 38 1081.5 39 1040.0 40 1025.5 41 1011.0 42 1042.5 43 1074.0 44 1106.5 45 1139.0 46 1412.5 47 1931.5 48 2177.0 49 2269.5 50 2362.0 51 2281.5 52 2201.0 53 2075.0 54 2013.0 55 2077.0 56 2100.5 57 2124.0 58 2247.5 59 2371.0 60 2544.0 61 2717.0 62 2649.5 63 2354.5 64 2127.0 65 1958.5 66 1790.0 67 1569.0 68 1348.0 69 1184.5 70 1021.0 71 901.5 72 693.0 73 604.0 74 501.0 75 398.0 76 312.0 77 226.0 78 170.0 79 79.0 80 44.0 81 35.5 82 27.0 83 19.5 84 12.0 85 7.5 86 3.0 87 1.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.016137491426957678 14 0.0020171864283697097 15 0.2218905071206681 16 0.046395287852503325 17 0.0 18 0.0040343728567394195 19 0.10489369427522492 20 0.09480776213337637 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.022189050712066807 27 0.09480776213337637 28 0.11094525356033405 29 0.046395287852503325 30 0.02824060999717594 31 0.03630935571065478 32 0.09480776213337637 33 0.17347803283979504 34 0.05849840642272159 35 0.05244684713761246 36 0.04236091499576391 37 0.17751240569653445 38 0.27030298140154113 39 0.1976842699802316 40 0.7221527413563561 41 0.869407350627345 42 0.1412030499858797 43 0.22995925283414692 44 0.4841247428087304 45 0.20978738855044984 46 0.1997014564086013 47 0.0403437285673942 48 0.17549521926816478 49 0.47202162423851207 50 0.15128898212772823 51 0.31468108282567475 52 0.12909993141566142 53 0.16944365998305563 54 0.250131117117844 55 0.25618267640295317 56 0.21785613426392866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 49574.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.47855730826643 #Duplication Level Percentage of deduplicated Percentage of total 1 86.42814582091978 64.37043611570581 2 8.090027625805753 12.050671723080647 3 2.505281404040951 5.597692338725945 4 1.121282704078869 3.3404607253802396 5 0.5687665890255132 2.1180457497881955 6 0.3818861383457018 1.7065397184007747 7 0.20854774930935488 1.0872634848912737 8 0.13542061643464604 0.8068745713478839 9 0.13542061643464604 0.9077338927663695 >10 0.3845945506743947 4.441844515270101 >50 0.0270841232869292 1.5532335498446765 >100 0.0135420616434646 2.0192036147980796 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 270 0.5446403356598217 No Hit CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 238 0.48009036995199095 No Hit CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 202 0.4074716585306814 No Hit CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 173 0.3489732521079598 No Hit CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 118 0.23802799854762577 No Hit CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 98 0.1976842699802316 No Hit CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 90 0.1815467785532739 No Hit CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 88 0.17751240569653445 No Hit CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 87 0.17549521926816478 No Hit CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 78 0.15734054141283738 No Hit CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 72 0.14523742284261912 No Hit CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 68 0.13716867712914027 No Hit CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 67 0.13515149070077057 No Hit CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 66 0.13313430427240086 No Hit CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 56 0.11296243998870376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.01412030499858797 0.0 0.0 0.0 0.0 7 0.01412030499858797 0.0 0.0 0.0 0.0 8 0.01412030499858797 0.0 0.0 0.0 0.0 9 0.01412030499858797 0.0 0.0 0.0 0.0 10 0.02218905071206681 0.0 0.0 0.0 0.0 11 0.02218905071206681 0.0 0.0 0.0 0.0 12 0.02218905071206681 0.0 0.0 0.0 0.0 13 0.032274982853915356 0.0 0.0 0.0 0.0 14 0.03832654213902449 0.0 0.0 0.0 0.0 15 0.04437810142413362 0.0 0.0 0.0 0.0 16 0.05042966070924275 0.0 0.0 0.0 0.0 17 0.05648121999435188 0.0 0.0 0.0 0.0 18 0.05849840642272159 0.0 0.0 0.0 0.0 19 0.062532779279461 0.0 0.0 0.0 0.0 20 0.06454996570783071 0.0 0.0 0.0 0.0 21 0.06858433856457014 0.0 0.0 0.0 0.0 22 0.08068745713478839 0.0 0.0 0.0 0.0 23 0.08673901641989752 0.0 0.0 0.0 0.0 24 0.08875620284826724 0.0 0.0 0.0 0.0 25 0.1028765078468552 0.0 0.0 0.0 0.0 26 0.11296243998870376 0.0 0.0 0.0 0.0 27 0.11699681284544318 0.0 0.0 0.0 0.0 28 0.12304837213055231 0.0 0.0 0.0 0.0 29 0.12304837213055231 0.0 0.0 0.0 0.0 30 0.12304837213055231 0.0 0.0 0.0 0.0 31 0.12304837213055231 0.0 0.0 0.0 0.0 32 0.12304837213055231 0.0 0.0 0.0 0.0 33 0.12708274498729172 0.0 0.0 0.0 0.0 34 0.12909993141566142 0.0 0.0 0.0 0.0 35 0.13111711784403116 0.0 0.0 0.0 0.0 36 0.13313430427240086 0.0 0.0 0.0 0.0 37 0.13515149070077057 0.0 0.0 0.0 0.0 38 0.13716867712914027 0.0 0.0 0.0 0.0 39 0.13918586355750998 0.0 0.0 0.0 0.0 40 0.1412030499858797 0.0 0.0 0.0 0.0 41 0.14523742284261912 0.0 0.0 0.0 0.0 42 0.14725460927098882 0.0 0.0 0.0 0.0 43 0.14927179569935853 0.0 0.0 0.0 0.0 44 0.15532335498446767 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTACT 20 3.975758E-4 50.422882 39 ACTTCCT 15 0.008108702 50.422882 40 TACGCCC 40 2.406523E-9 50.422882 38 TTTACTT 20 3.975758E-4 50.422882 40 GCTTGTC 25 1.958028E-5 50.42288 38 GCCCAGT 35 4.859612E-8 50.37143 41 TGTCTCA 25 1.9699191E-5 50.37143 41 CCACAGT 45 1.2187229E-10 50.37143 41 TTACTTT 20 3.9958768E-4 50.371426 41 ATATGCT 35 4.9385562E-8 50.268837 34 CAGCAAA 15 0.008240644 50.2177 47 TTCGCGC 15 0.008240644 50.2177 49 CTTTGAG 15 0.008240644 50.2177 49 CTTCCGA 15 0.008240644 50.2177 46 GATAGAA 15 0.008307211 50.115738 43 CTTACTG 15 0.008340645 50.064907 50 CTCTCTA 15 0.008374178 50.014187 32 CATATGC 40 2.586603E-9 50.014183 33 TGTCCCG 15 0.008407812 49.963562 15 CCAGGAC 15 0.008407812 49.963562 16 >>END_MODULE