FastQCFastQC Report
Fri 17 Jun 2016
SRR1526261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48862
Sequences flagged as poor quality0
Sequence length56
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2070.42364209406082437No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1480.3028938643526667No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1180.24149645941631534No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC870.17805247431541893No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC780.15963325283451352No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG670.13712087102451803No Hit
TGCAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.13507429085997297No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG490.10028242806270722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC150.00799895950.59541338
GCTTGTC150.00799895950.59541338
CGCTGTA150.00816533750.3329943
TGCCTAG251.9783483E-550.33298543
CTTTGCC204.0102997E-450.33298544
ACTGATT150.0081989250.2808342
TTGCCTA204.0309102E-450.28082742
AGATTAA309.951491E-750.2287849
TATGCTT204.051605E-450.22877535
AGAGCGC204.051605E-450.22877535
CCTAGCA252.0151416E-550.17683845
CCCGCTG150.00830028250.12550
ATATGCT301.0167241E-650.07327334
ATATGCC150.00833427650.07327334
CGAAAGG150.00833427650.07327315
GGTTGAT451.2914825E-1050.0732715
CTTAATC204.114196E-450.0732715
TCTTAAT204.114196E-450.0732714
GGTAAGC204.114196E-450.0732734
TAAGCAA150.00836837450.02165232