SRR1526260_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78813
Sequences flagged as poor quality	0
Sequence length	56
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	894	1.1343306307335084	No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	311	0.3946049509598671	No Hit
TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	305	0.386991993706622	No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	264	0.334970119142781	No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	260	0.32989481430728435	No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	233	0.2956365066676817	No Hit
TTCCGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	197	0.24995876314821158	No Hit
TTCCGCGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT	152	0.1928615837488739	No Hit
TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC	142	0.18017332166013222	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	132	0.1674850595713905	No Hit
TTCCGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	130	0.16494740715364217	No Hit
TTCCGCGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT	128	0.16240975473589384	No Hit
TTCCGCGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG	113	0.14337736160278128	No Hit
TTCCGCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	111	0.1408397091850329	No Hit
TTCCGCGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA	105	0.1332267519317879	No Hit
TTCCGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC	100	0.12688262088741706	No Hit
TTCCGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC	96	0.12180731605192037	No Hit
TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC	94	0.11926966363417202	No Hit
TTCCGCGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGCTCGCACGCCCCCCGT	92	0.11673201121642368	No Hit
TTCCGCGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT	92	0.11673201121642368	No Hit
TTCCGCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	91	0.11546318500754951	No Hit
TTCCGCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC	87	0.11038788017205282	No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	87	0.11038788017205282	No Hit
TTCCGCGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGA	85	0.1078502277543045	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	85	0.1078502277543045	No Hit
TTCCGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	83	0.10531257533655616	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	80	0.10150609670993363	No Hit
TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCCCTAATACATGCCGAC	79	0.10023727050105946	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	50	5.456968E-12	50.359436	48
ACAAAAC	30	9.853356E-7	50.359432	48
CAACGTC	15	0.008167189	50.359432	49
CCAACGT	15	0.008167189	50.359432	48
CTGATCT	15	0.008167189	50.359432	49
CTAATCG	15	0.008167189	50.359432	49
ATGATCC	15	0.008187948	50.32713	46
CAACGAT	35	4.9440132E-8	50.32713	46
AGCTAAA	15	0.008187948	50.32713	46
CGACCAC	25	1.9987174E-5	50.294876	37
TCATACC	25	1.9987174E-5	50.294876	43
TGCTCGT	45	1.2551027E-10	50.29487	37
CGTCTCA	50	7.2759576E-12	50.262657	41
TCGAGCT	15	0.008229583	50.26265	39
ACTTTGT	15	0.008229583	50.26265	40
ATGCCCG	15	0.008229583	50.26265	36
ATATGCC	50	7.2759576E-12	50.166245	34
CTCCCGC	15	0.008292331	50.16624	34
AAGCGCA	20	4.1442245E-4	50.038265	50
AAGTGGT	40	2.6702764E-9	49.910942	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content