##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526260_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 78813 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.5159428013145 39.0 38.0 40.0 36.0 40.0 2 38.485376777942726 39.0 38.0 40.0 36.0 40.0 3 38.26075647418573 39.0 38.0 40.0 35.0 40.0 4 38.3888191034474 39.0 38.0 40.0 36.0 40.0 5 37.461878116554374 39.0 38.0 39.0 33.0 40.0 6 37.87032596145306 39.0 38.0 39.0 35.0 40.0 7 38.01853755091165 39.0 38.0 39.0 35.0 40.0 8 38.10362503647875 39.0 38.0 40.0 35.0 40.0 9 38.19032393133113 39.0 38.0 40.0 35.0 40.0 10 37.36790884752516 39.0 37.0 39.0 35.0 40.0 11 35.85644500272797 38.0 35.0 39.0 29.0 40.0 12 34.5267658888762 38.0 33.0 39.0 23.0 40.0 13 34.42095847131819 38.0 33.0 39.0 23.0 40.0 14 34.41962620379886 38.0 33.0 39.0 23.0 40.0 15 34.398855518759596 38.0 33.0 39.0 23.0 40.0 16 33.92159922855367 38.0 33.0 39.0 22.0 40.0 17 33.83701927347011 38.0 32.0 39.0 22.0 40.0 18 33.33791379594737 37.0 31.0 39.0 20.0 40.0 19 34.224188902845974 38.0 33.0 39.0 25.0 40.0 20 34.18352302285156 38.0 32.0 39.0 25.0 40.0 21 34.853843908999785 38.0 33.0 39.0 26.0 40.0 22 34.520155304327965 38.0 33.0 39.0 25.0 40.0 23 35.01569538020377 38.0 34.0 39.0 27.0 40.0 24 34.49702460254019 38.0 33.0 39.0 25.0 40.0 25 34.73907857840712 38.0 33.0 39.0 26.0 40.0 26 35.0932079733039 38.0 35.0 39.0 27.0 40.0 27 34.987463997056324 38.0 35.0 39.0 27.0 40.0 28 34.83747605090531 38.0 34.0 39.0 27.0 40.0 29 34.63282707167599 38.0 33.0 39.0 26.0 40.0 30 34.44585284153629 38.0 33.0 39.0 25.0 40.0 31 34.610622613020695 38.0 34.0 39.0 27.0 40.0 32 34.29222336416581 38.0 33.0 39.0 25.0 40.0 33 34.41281260705721 38.0 33.0 39.0 26.0 40.0 34 34.25330846433964 38.0 33.0 39.0 26.0 40.0 35 34.1470696458706 38.0 33.0 39.0 25.0 40.0 36 33.83693045563549 37.0 33.0 38.0 26.0 40.0 37 33.58572824280258 36.0 33.0 38.0 25.0 39.0 38 33.286703970157205 36.0 32.0 38.0 23.0 39.0 39 32.81491632091152 36.0 32.0 38.0 22.0 39.0 40 29.75655031530331 35.0 25.0 38.0 9.0 39.0 41 26.77916079834545 32.0 16.0 38.0 6.0 39.0 42 30.352784439115375 34.0 28.0 38.0 10.0 39.0 43 28.97369723269004 33.0 25.0 38.0 5.0 39.0 44 28.985522692956746 33.0 25.0 38.0 4.0 39.0 45 29.991574993973074 34.0 28.0 38.0 4.0 39.0 46 29.491035742834303 34.0 26.0 38.0 4.0 39.0 47 29.066473805082918 34.0 26.0 38.0 4.0 39.0 48 29.078400771446336 34.0 26.0 38.0 4.0 39.0 49 26.762564551533377 33.0 16.0 38.0 4.0 39.0 50 28.59713499042036 33.0 25.0 38.0 4.0 39.0 51 28.892378160963293 35.0 26.0 38.0 4.0 40.0 52 28.54262621648713 34.0 25.0 38.0 4.0 40.0 53 28.111250681994086 34.0 23.0 38.0 4.0 40.0 54 27.12764391661274 33.0 17.0 38.0 4.0 39.0 55 27.49772245695507 33.0 21.0 38.0 4.0 39.0 56 26.45568624465507 33.0 16.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6798615974129731 1 2 -0.5122970716502948 1 3 -0.6482540094031606 1 4 -0.47743023682292574 1 5 0.17778394096250594 1 6 -0.10760563664554468 1 7 -0.144014562001459 1 8 -0.4272472186641849 1 9 -0.23515091540130584 1 10 -0.06086039507793117 1 11 -4.4885224864109325 1 12 -14.401240799655636 1 13 -10.396606977202158 1 14 -5.924079808009608 1 15 -6.853627848985351 1 16 -23.559533444873225 1 17 -4.689925661248644 1 18 -12.307703335726895 1 19 -5.167470311041178 1 20 -5.290142542772305 1 21 -5.394335960187341 1 22 -2.4974775248190113 1 23 -3.4565896676875383 1 24 -3.6713526859192847 1 25 -3.4318756666607975 1 26 1.768399890372514 1 27 2.2304677507141477 1 28 2.0646542757662374 1 29 1.919826065245502 1 30 2.414778861969843 1 31 1.3645808529880057 1 32 1.7672186364339595 1 33 1.6054340078971805 1 34 0.9121872793500998 1 35 1.4307864987979713 1 36 1.9946816532094331 1 37 2.138076072370197 1 38 1.900404982018685 1 39 2.5680436589312947 1 40 4.195930421290445 1 41 -9.120751027494006 1 42 -7.678453084206662 1 43 -16.644639314886714 1 44 -21.583952277510303 1 45 -11.691264141690969 1 46 -17.376859719012664 1 47 -22.078427658694007 1 48 -14.527503520876499 1 49 -19.760041857394107 1 50 -13.03933830701697 1 51 -11.014785384554198 1 52 -10.445361596608883 1 53 -9.602713109147267 1 54 -9.477156397506377 1 55 -9.562965534932722 1 56 -5.450070138436235 2 1 0.3592923179785643 2 2 0.3875679148483542 2 3 0.4667324892467022 2 4 0.4495499611968796 2 5 0.9718883514035497 2 6 0.9861162355416724 2 7 0.6234246819229341 2 8 0.34964297035471503 2 9 0.6423243321234438 2 10 0.33045373633520825 2 11 1.1630301688545899 2 12 -3.976848360411708 2 13 -1.5614359601004466 2 14 2.6390390038715807 2 15 2.8766826820677522 2 16 2.5216996784391057 2 17 3.049385769894471 2 18 -2.4932343888322066 2 19 2.6242713631262404 2 20 2.5400779792799 2 21 2.446010574466122 2 22 2.8487195948929624 2 23 2.8696929605752857 2 24 1.9116806174110437 2 25 2.4359236132671924 2 26 2.763674417825257 2 27 2.6472094248815665 2 28 2.7622240327419334 2 29 2.7906631489538753 2 30 2.53617850193384 2 31 2.1424836432670276 2 32 1.7780197165419693 2 33 1.9554565101474068 2 34 0.370108071429307 2 35 0.9427126914172348 2 36 1.383858070851197 2 37 1.1711543802009814 2 38 1.4932267641969048 2 39 1.5891957741428158 2 40 4.941429971245444 2 41 -6.48416236862812 2 42 -7.253835622460489 2 43 -15.379337784733698 2 44 -14.470428425125062 2 45 -6.406160631339933 2 46 -11.96236826986775 2 47 -17.62692000792893 2 48 -9.731823952919704 2 49 -13.239339787187085 2 50 -8.83378025121139 2 51 -3.851306536669412 2 52 -4.355465106959919 2 53 -4.5354313809744475 2 54 -2.705441726039229 2 55 -3.1057198103602666 2 56 -0.8461097423966315 3 1 0.778180360628987 3 2 0.8058847465315253 3 3 1.004718018568866 3 4 0.9071851477924611 3 5 0.8569797451583128 3 6 1.312848908809002 3 7 0.9914749484880545 3 8 0.7816688652519019 3 9 1.187926007675614 3 10 0.9408878566703152 3 11 3.0927712198827706 3 12 4.540192766777935 3 13 3.236260155664972 3 14 4.0374586535288515 3 15 4.291477046119546 3 16 4.398508513168736 3 17 5.207801611478629 3 18 5.452786174762615 3 19 3.897215003644135 3 20 4.410032282402518 3 21 3.846048655197272 3 22 4.413361636020149 3 23 4.0591445980467284 3 24 4.3295214399548385 3 25 3.339103354318226 3 26 3.5586096805823004 3 27 3.4393838346302346 3 28 2.541927003038964 3 29 3.6470987925182285 3 30 3.29065298784397 3 31 3.2544409928481457 3 32 2.5434424126577397 3 33 2.8056088330720073 3 34 2.191033433196253 3 35 2.436905379916851 3 36 2.69135997488776 3 37 3.45276138705551 3 38 3.727328058941765 3 39 4.020840861728502 3 40 7.535965386325412 3 41 8.690437783694808 3 42 5.245497964744388 3 43 0.08001103476363625 3 44 3.1345791910211673 3 45 6.321847746420918 3 46 3.131881539728596 3 47 -1.9744066796730273 3 48 3.9716223532493764 3 49 6.14118191882967 3 50 4.586535818857154 3 51 6.332242587473772 3 52 6.695372669125383 3 53 6.804629548195393 3 54 6.980011434661453 3 55 6.470251248284061 3 56 6.876852938486845 4 1 0.5185649760136002 4 2 0.4693462849930654 4 3 0.379718018568866 4 4 0.41680053240784076 4 5 0.7896720528506194 4 6 0.7263104472705422 4 7 0.4241672561803611 4 8 0.5508996344826684 4 9 0.5821567769063876 4 10 0.5370417028241619 4 11 2.4773866044981574 4 12 3.7517312283163946 4 13 3.072798617203439 4 14 3.9028432689134718 4 15 3.6760924307349256 4 16 4.465816205476422 4 17 4.28472468840171 4 18 4.279709251685688 4 19 3.137599619028755 4 20 3.5446476670179052 4 21 2.7114332705818924 4 22 2.4999000975586156 4 23 1.9437599826621081 4 24 2.7814445168779187 4 25 2.9064110466259194 4 26 2.0105327575053806 4 27 2.4105376807840813 4 28 1.878465464577424 4 29 1.8970987925182285 4 30 2.04065298784397 4 31 1.7736717620789122 4 32 2.8030577972731194 4 33 -0.28092962846645264 4 34 0.4121872793500998 4 35 0.8888284568399314 4 36 0.7394368979646799 4 37 1.07776138705551 4 38 1.486943443557145 4 39 1.4439177848054214 4 40 5.160965386325412 4 41 7.892360860617881 4 42 4.062805657052074 4 43 5.560780265532866 4 44 4.384579191021164 4 45 5.071847746420918 4 46 4.612650770497826 4 47 3.4871317818654326 4 48 4.50046850709553 4 49 7.19887422652198 4 50 4.413458895780234 4 51 4.341857972089159 4 52 4.66652651527923 4 53 3.42962954819539 4 54 5.143472973122993 4 55 4.047174325207138 4 56 3.838391400025305 5 1 0.04741112985975349 5 2 0.3205906288844673 5 3 0.36199554496403863 5 4 0.3240403514123713 5 5 0.7825076486273872 5 6 0.6265743990050794 5 7 0.12759862872938044 5 8 0.6206583071825165 5 9 0.24279026106928114 5 10 0.1686404961876633 5 11 1.501896408419725 5 12 3.751165617456664 5 13 3.4253627197675414 5 14 3.8332731331668626 5 15 3.667985341745485 5 16 4.535386341223031 5 17 4.560742787949216 5 18 4.576654953043153 5 19 3.6843567834329747 5 20 4.04163107576602 5 21 2.5296836476557942 5 22 2.579839765733574 5 23 2.4961732556636207 5 24 2.9948683479488096 5 25 2.4226252246048006 5 26 2.1155478404616375 5 27 2.2170987667569264 5 28 0.938985826568377 5 29 2.039255655263325 5 30 2.5540391115242116 5 31 1.9354364679612672 5 32 1.8911045544375256 5 33 0.2999527244747213 5 34 0.24061865189911913 5 35 2.113941579011872 5 36 1.4164730970597006 5 37 1.8550992452757242 5 38 2.010699099665743 5 39 1.1999509673091922 5 40 5.846108674409873 5 41 7.107858598174442 5 42 4.174042459465348 5 43 4.917303644115069 5 44 3.9345414836305217 5 45 3.615776856526498 5 46 3.7657427765310096 5 47 4.424914587295298 5 48 4.360197013882857 5 49 6.197177393942791 5 50 4.335216060184454 5 51 3.98231800225507 5 52 3.2892640718403143 5 53 3.9102102420113773 5 54 4.047130590015904 5 55 3.537747477544997 5 56 2.2264004497990584 6 1 0.3551034375520601 6 2 0.3731924388392187 6 3 0.5143334031842528 6 4 0.47449284010015447 6 5 0.8281335913121524 6 6 0.6590027549628488 6 7 0.3280134100265144 6 8 0.570130403713442 6 9 0.5436952384448475 6 10 0.29665708743954866 6 11 2.198540450652004 6 12 3.7613466129317814 6 13 3.736260155664972 6 14 3.768227884298085 6 15 3.0991693538118525 6 16 4.138893128553349 6 17 4.438570842247863 6 18 4.356632328608768 6 19 3.9452919267210618 6 20 2.929263051633292 6 21 2.567202501351119 6 22 2.7883616360201486 6 23 2.251452290354422 6 24 2.8295214399548385 6 25 2.0314110466259194 6 26 1.2605327575053806 6 27 1.6124607577071544 6 28 1.0323116184235772 6 29 1.4067141771336154 6 30 1.1656529878439699 6 31 0.908287146694299 6 32 1.1395962588115864 6 33 0.46907037153354736 6 34 0.0468026639654866 6 35 0.8792130722245446 6 36 -0.12594771741993327 6 37 0.5489152332093568 6 38 0.03502036663407182 6 39 0.28045624634388133 6 40 4.5840423094023315 6 41 -0.4441776009205789 6 42 2.235882580129001 6 43 4.00308795784056 6 44 2.298040729482704 6 45 2.2641554387286114 6 46 2.6991892320362894 6 47 3.5640548587885093 6 48 2.8754685070955297 6 49 4.862335764983516 6 50 2.730766588087924 6 51 2.0630118182430017 6 52 3.0319111306638433 6 53 2.2661680097338532 6 54 3.3165498961999162 6 55 1.681789709822521 6 56 1.6845452461791481 7 1 0.15347173592036256 7 2 0.26295545360223116 7 3 0.5108368996877459 7 4 0.2839333995407074 7 5 0.33182434500290725 7 6 0.6360812320413274 7 7 0.4027026097157176 7 8 0.2659345995176352 7 9 0.334293529043137 7 10 -0.022981607199149323 7 11 1.9395078166193684 7 12 3.674516776101939 7 13 3.20314049754532 7 14 3.7640515051217065 7 15 4.205715585358085 7 16 4.473294837955059 7 17 4.086589490266505 7 18 4.756690603667046 7 19 3.3388428202719567 7 20 3.5368776592478994 7 21 2.9200579792065966 7 22 2.5643911620496738 7 23 2.396945685847811 7 24 3.0129907484241514 7 25 2.9885788787937457 7 26 2.079005950978569 7 27 2.129457649704051 7 28 1.9030381141500783 7 29 1.8339674793869136 7 30 2.2670515892425698 7 31 1.680237418644566 7 32 2.1914610606763816 7 33 1.7102319876951668 7 34 1.2126923298551517 7 35 1.9333117513232239 7 36 1.296449329977115 7 37 1.5395912238853526 7 38 1.851162557776263 7 39 2.8054173963050317 7 40 4.966132441492466 7 41 -0.5121651689081475 7 42 4.460435804682227 7 43 5.049805129557729 7 44 4.816300247742223 7 45 4.821362120935294 7 46 5.224150381997436 7 47 5.720814765548418 7 48 4.674516681143704 7 49 8.396523799171554 7 50 5.060409167730501 7 51 5.9157701710013555 7 52 5.295800019552729 7 53 6.334155577721418 7 54 6.824611279261301 7 55 6.336024364057177 7 56 5.610535922169827 8 1 0.17117350609737514 8 2 0.2786570237592514 8 3 0.5162374397417508 8 4 0.1723222384245986 8 5 -0.1469235297845728 8 6 0.570274651383258 8 7 0.09867220667540977 8 8 0.2902370297606609 8 9 0.275987698460078 8 10 0.37005769673124433 8 11 1.8759014559833034 8 12 3.9736466890932434 8 13 3.785098693364901 8 14 3.6192370236735627 8 15 4.500445058305374 8 16 4.095957104181679 8 17 4.158296660983581 8 18 4.922607195326208 8 19 4.079716907680698 8 20 3.411365107992772 8 21 2.8420501784265184 8 22 3.19525424835831 8 23 2.929098901169347 8 24 3.2087103203813427 8 25 2.3864186627721438 8 26 1.912189269310403 8 27 1.6472094248815665 8 28 1.8216299733359946 8 29 2.3847225548944664 8 30 2.169841868270474 8 31 2.053374732375943 8 32 2.5799999145617747 8 33 1.123773341830578 8 34 0.9047615367758439 8 35 1.5565740775558439 8 36 1.0472244074848618 8 37 1.2899662613890968 8 38 1.2259000315236364 8 39 2.0149383484002428 8 40 3.802816109859304 8 41 -3.088122764667723 8 42 2.478837644866246 8 43 5.214721621206895 8 44 4.371155733290781 8 45 3.3067106557887804 8 46 4.166344601419379 8 47 4.145357219793844 8 48 4.268176047080299 8 49 6.027986945486184 8 50 3.5127544022539574 8 51 4.722950889073161 8 52 3.3871091504658253 8 53 5.038826044768122 8 54 5.452974115544929 8 55 4.7556663282535965 8 56 5.391514019979606 9 1 -0.6056500946300432 9 2 0.05588474653152531 9 3 -0.3286807255441673 9 4 -0.31161391029231567 9 5 0.24689340292911055 9 6 0.19039992921716475 9 7 -0.307975600962493 9 8 0.04422773495362975 9 9 0.012298064190531477 9 10 0.17244201679590532 9 11 2.298815175926727 9 12 3.4336386066523445 9 13 3.479784488475019 9 14 3.1345936613781475 9 15 3.8650641732780997 9 16 3.825902547705624 9 17 2.993515897192914 9 18 4.431396849801857 9 19 2.9774740303317344 9 20 1.6474734127007906 9 21 2.4602558765473503 9 22 2.6066504900232914 9 23 2.780887141217839 9 24 2.253971989405386 9 25 2.599896132968148 9 26 1.5833349553075777 9 27 1.2759222961686945 9 28 1.8123351663042726 9 29 0.6368947108855778 9 30 1.0314300679067685 9 31 0.9726513539156443 9 32 0.9351221614803436 9 33 0.20631526949273393 9 34 0.049942381390913226 9 35 0.07132453219314527 9 36 -0.36083782731004277 9 37 0.0865918423145402 9 38 0.16316008405949844 9 39 0.08010302813352155 9 40 2.596208714425881 9 41 0.13667012278428814 9 42 2.7876879176486256 9 43 4.38829203947321 9 44 3.3058900230462847 9 45 2.8314631310363048 9 46 3.1314890750504176 9 47 2.7214331947382746 9 48 3.3277840486967847 9 49 5.571126973774724 9 50 3.50588432749138 9 51 2.872273984648025 9 52 3.8252784776026196 9 53 3.0322590615391896 9 54 4.577342674849838 9 55 2.5993721274049406 9 56 3.254011494216826 10 1 0.5780233747577128 10 2 0.6783337261233626 10 3 0.7223396826191006 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1.3412917569620433 119 35 2.3483577335333834 119 36 2.8449191023274807 119 37 3.127560468571012 119 38 2.648539310376897 119 39 3.1101060741280406 119 40 6.38283679849533 119 41 3.3348120661287908 119 42 -1.8043011167711143 119 43 -9.166688161562426 119 44 -7.29782609026471 119 45 -5.5118204510532465 119 46 -16.4413101939109 119 47 -13.65322413087854 119 48 -13.882998771894139 119 49 -12.013094774626127 119 50 -11.387201264954555 119 51 -9.269364760402233 119 52 -8.6677157350078 119 53 -10.960582851345368 119 54 -10.480378921251289 119 55 -8.173548981337063 119 56 -7.017234317540712 120 1 -1.5102811778325531 120 2 -1.521038330391555 120 3 -1.158743519892674 120 4 -1.506276390669079 120 5 -0.9795587163801542 120 6 -0.8409972450371512 120 7 -0.989294282281179 120 8 -1.3721772885942514 120 9 -1.0909201461705393 120 10 -1.2514198356373782 120 11 -3.3110749339633827 120 12 -11.123268771683605 120 13 -8.407970613565794 120 14 -10.837541346471149 120 15 -11.583522953880458 120 16 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54 -13.308450103800082 120 55 -11.972056444023632 120 56 -9.296223984590082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 67.0 5 18.0 6 36.0 7 35.0 8 115.0 9 288.0 10 156.0 11 158.0 12 160.0 13 104.0 14 148.0 15 191.0 16 214.0 17 267.0 18 374.0 19 517.0 20 564.0 21 702.0 22 869.0 23 1126.0 24 1388.0 25 1609.0 26 1820.0 27 1904.0 28 2279.0 29 2533.0 30 3094.0 31 3660.0 32 4211.0 33 4855.0 34 5650.0 35 6771.0 36 8399.0 37 10225.0 38 9272.0 39 5034.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.12450991587683 0.7346503749381448 0.10023727050105946 0.04060243868397346 8 99.43283468463324 0.4225191275550988 0.06724778907033103 0.07739839874132441 9 98.77431388222755 0.6724778907033104 0.24995876314821158 0.3032494639209268 10 55.85246088843211 25.52624567013056 9.117785136969788 9.503508304467537 11 36.910154416149624 29.36952025681043 17.725502137972164 15.994823189067795 12 32.15713143770698 13.618311699846473 21.402560491289506 32.82199637115704 13 24.186506929285752 14.17711558962384 24.987309000456875 36.64906848063353 14 22.49530528345937 18.882911231792114 31.471095772217428 27.150687712531084 15 26.370342108609947 20.218746025689942 26.5712832252321 26.839628640468018 16 32.91055671935504 20.238684694978733 23.058465054275377 23.792293531390847 17 29.826297692005127 19.956098613172955 27.008234682095594 23.209369012726327 18 27.047911485560572 18.83723290869411 28.65553469014871 25.45932091559661 19 32.62796900800203 23.17159913628858 20.94754223294805 23.252889622761337 20 28.97473997028232 27.022770856351837 21.23925273999568 22.763236433370164 21 27.467549769708043 21.440625277555732 27.397764328219964 23.69406062451626 22 24.471851090556125 20.08551888647812 26.589522033167118 28.853107989798637 23 24.37795795109944 22.61048304213772 24.171139279052948 28.840419727709893 24 24.42363569461891 23.416187684772815 27.12877317193864 25.031403448669636 25 28.739642943243965 25.30991866617604 21.274948928449074 24.67548946213092 26 25.011103356386016 22.70668104815684 25.103737072520776 27.178478522936363 27 24.091763629896853 20.21873888521925 25.057161729586912 30.632335755296985 28 24.214164104388484 23.496302696109574 26.388077147866746 25.901456051635197 29 29.806679275459818 21.32620809586068 24.526218250593416 24.340894378086087 30 26.36782455294252 20.590661606995546 29.819907859835265 23.22160598022667 31 25.853534712526972 23.05114862292169 28.1266658205356 22.96865084401574 32 30.335937003873752 20.821743824220487 26.175144471962913 22.667174699942848 33 26.06507371631927 21.21377732587697 27.19369598373157 25.52745297407219 34 23.315983242351148 25.000634759426177 31.47391138758411 20.20947061063857 35 24.17356421063322 22.6171736149901 34.489412481592446 18.719849692784237 36 25.990378634984708 25.018087658505006 28.37541093890814 20.616122767602146 37 24.995232343779797 20.612802746169983 32.350136672811644 22.041828237238573 38 28.20692288114024 23.857215576482567 26.422753881394755 21.51310766098244 39 25.51806554959445 21.64433369778026 25.926161356759643 26.911439395865642 40 20.20056208482371 16.922585590189065 26.388605007664793 36.48824731732243 41 17.635914438228856 15.449506522100897 25.99239621602939 40.92218282364086 42 24.484559001003568 21.343004865407337 29.74377215157713 24.428663982011965 43 19.327186009538952 16.90302066772655 33.41430842607313 30.355484896661366 44 21.290594040469724 18.623213352203905 28.06232388529753 32.023868722028844 45 23.696357047491894 21.969610274016148 31.377709962489668 22.95632271600229 46 22.10751156995372 22.989879469053555 29.28215429995423 25.6204546610385 47 20.323170267319945 24.225076794191565 31.997156710923814 23.45459622756467 48 23.196858798414148 24.599725526075023 29.092965334959846 23.11045034055098 49 19.494552598678336 20.266374097415355 25.250682519837724 34.988390784068585 50 25.859199308828945 21.16203133139365 29.44719021179819 23.531579147979215 51 24.20366764657224 25.112307359472634 29.602056529098103 21.081968464857024 52 22.727388211382113 22.873475609756095 31.50025406504065 22.898882113821138 53 23.161400208455145 20.534102753133183 32.41985916567099 23.884637872740676 54 21.9500407000407 22.423178673178672 27.654405779405778 27.972374847374848 55 23.582157892728592 24.988870941073223 29.63890973379291 21.790061432405274 56 24.51153626136147 21.02205555202441 29.890040043221255 24.57636814339287 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.5 11 3.0 12 14.5 13 54.0 14 82.0 15 150.0 16 218.0 17 216.0 18 214.0 19 258.0 20 302.0 21 375.5 22 434.5 23 420.0 24 460.0 25 500.0 26 589.5 27 679.0 28 807.5 29 1094.0 30 1252.0 31 1347.5 32 1443.0 33 1508.5 34 1574.0 35 1515.5 36 1457.0 37 1364.0 38 1231.5 39 1192.0 40 1179.5 41 1167.0 42 1201.5 43 1236.0 44 1312.0 45 1388.0 46 1785.5 47 3078.5 48 3974.0 49 3830.5 50 3687.0 51 3297.0 52 2907.0 53 2988.5 54 3326.5 55 3583.0 56 3771.5 57 3960.0 58 4000.5 59 4041.0 60 4400.5 61 4760.0 62 5400.0 63 5731.0 64 5422.0 65 5093.0 66 4764.0 67 4359.0 68 3954.0 69 3435.0 70 2916.0 71 2676.0 72 2129.0 73 1822.0 74 1680.0 75 1538.0 76 1200.0 77 862.0 78 692.0 79 406.5 80 291.0 81 230.0 82 169.0 83 123.5 84 78.0 85 47.0 86 16.0 87 10.5 88 3.5 89 2.0 90 1.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.021570045550860898 14 0.0012688262088741706 15 0.2321951962239732 16 0.060903658025960185 17 0.0 18 0.0012688262088741706 19 0.10531257533655616 20 0.09135548703894027 21 0.0 22 0.0 23 0.0 24 0.0 25 0.002537652417748341 26 0.010150609670993365 27 0.11292553258980118 28 0.13576440434953624 29 0.04060243868397346 30 0.025376524177483407 31 0.02918300280410592 32 0.0989684442921853 33 0.16875388578026468 34 0.054559526981589335 35 0.05202187456384099 36 0.038064786266225115 37 0.20047454100211892 38 0.2956365066676817 39 0.19666806237549642 40 0.6762843693299329 41 0.8805653889586743 42 0.11926966363417202 43 0.23853932726834404 44 0.4872292642076815 45 0.21316280309086064 46 0.20428101962874146 47 0.03933361247509928 48 0.14845266643827795 49 0.5417887911892708 50 0.13449557814066207 51 0.2969053328765559 52 0.11800083742529786 53 0.1763668430335097 54 0.2398081534772182 55 0.2410769796860924 56 0.18778627891337724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 78813.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.26616167383554 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7804938843093 61.84512707294482 2 7.771467231648477 11.076852803471509 3 2.4391546637705415 5.214875718472841 4 1.0718037281677855 3.0553335109690027 5 0.5910944148699414 2.106251506731123 6 0.34539854362882116 1.4769137071295344 7 0.2350134420567237 1.1723954169997335 8 0.12996955507682445 0.7409945059825156 9 0.0943614577955027 0.6052301016329793 >10 0.4700268841134474 5.891160087802773 >50 0.044510121601652214 2.403156839607679 >100 0.02492566809692524 3.277378097521982 >500 0.0017804048640660886 1.1343306307335084 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 894 1.1343306307335084 No Hit TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 311 0.3946049509598671 No Hit TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 305 0.386991993706622 No Hit TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 264 0.334970119142781 No Hit TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 260 0.32989481430728435 No Hit TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 233 0.2956365066676817 No Hit TTCCGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 197 0.24995876314821158 No Hit TTCCGCGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT 152 0.1928615837488739 No Hit TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC 142 0.18017332166013222 No Hit TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 132 0.1674850595713905 No Hit TTCCGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 130 0.16494740715364217 No Hit TTCCGCGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT 128 0.16240975473589384 No Hit TTCCGCGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG 113 0.14337736160278128 No Hit TTCCGCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 111 0.1408397091850329 No Hit TTCCGCGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA 105 0.1332267519317879 No Hit TTCCGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 100 0.12688262088741706 No Hit TTCCGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC 96 0.12180731605192037 No Hit TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC 94 0.11926966363417202 No Hit TTCCGCGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGCTCGCACGCCCCCCGT 92 0.11673201121642368 No Hit TTCCGCGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT 92 0.11673201121642368 No Hit TTCCGCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 91 0.11546318500754951 No Hit TTCCGCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC 87 0.11038788017205282 No Hit TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 87 0.11038788017205282 No Hit TTCCGCGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGA 85 0.1078502277543045 No Hit TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 85 0.1078502277543045 No Hit TTCCGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 83 0.10531257533655616 No Hit TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 80 0.10150609670993363 No Hit TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCCCTAATACATGCCGAC 79 0.10023727050105946 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002537652417748341 0.0 0.0 0.0 0.0 11 0.006344131044370853 0.0 0.0 0.0 0.0 12 0.006344131044370853 0.0 0.0 0.0 0.0 13 0.010150609670993365 0.0 0.0 0.0 0.0 14 0.010150609670993365 0.0 0.0 0.0 0.0 15 0.015225914506490046 0.0 0.0 0.0 0.0 16 0.02030121934198673 0.0 0.0 0.0 0.0 17 0.021570045550860898 0.0 0.0 0.0 0.0 18 0.02791417659523175 0.0 0.0 0.0 0.0 19 0.031720655221854265 0.0 0.0 0.0 0.0 20 0.0342583076396026 0.0 0.0 0.0 0.0 21 0.036795960057350946 0.0 0.0 0.0 0.0 22 0.043140091101721796 0.0 0.0 0.0 0.0 23 0.04694656972834431 0.0 0.0 0.0 0.0 24 0.05329070077271516 0.0 0.0 0.0 0.0 25 0.059634831817086016 0.0 0.0 0.0 0.0 26 0.0647101366525827 0.0 0.0 0.0 0.0 27 0.07105426769695355 0.0 0.0 0.0 0.0 28 0.07612957253245023 0.0 0.0 0.0 0.0 29 0.07866722495019857 0.0 0.0 0.0 0.0 30 0.08120487736794692 0.0 0.0 0.0 0.0 31 0.08374252978569525 0.0 0.0 0.0 0.0 32 0.08501135599456942 0.0 0.0 0.0 0.0 33 0.08501135599456942 0.0 0.0 0.0 0.0 34 0.08628018220344359 0.0 0.0 0.0 0.0 35 0.08881783462119193 0.0 0.0 0.0 0.0 36 0.08881783462119193 0.0 0.0 0.0 0.0 37 0.09135548703894028 0.0 0.0 0.0 0.0 38 0.09389313945668862 0.0 0.0 0.0 0.0 39 0.09769961808331112 0.0 0.0 0.0 0.0 40 0.09896844429218529 0.0 0.0 0.0 0.0 41 0.10150609670993364 0.0 0.0 0.0 0.0 42 0.11038788017205284 0.0 0.0 0.0 0.0 43 0.11546318500754951 0.0 0.0 0.0 0.0 44 0.11546318500754951 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGATTA 50 5.456968E-12 50.359436 48 ACAAAAC 30 9.853356E-7 50.359432 48 CAACGTC 15 0.008167189 50.359432 49 CCAACGT 15 0.008167189 50.359432 48 CTGATCT 15 0.008167189 50.359432 49 CTAATCG 15 0.008167189 50.359432 49 ATGATCC 15 0.008187948 50.32713 46 CAACGAT 35 4.9440132E-8 50.32713 46 AGCTAAA 15 0.008187948 50.32713 46 CGACCAC 25 1.9987174E-5 50.294876 37 TCATACC 25 1.9987174E-5 50.294876 43 TGCTCGT 45 1.2551027E-10 50.29487 37 CGTCTCA 50 7.2759576E-12 50.262657 41 TCGAGCT 15 0.008229583 50.26265 39 ACTTTGT 15 0.008229583 50.26265 40 ATGCCCG 15 0.008229583 50.26265 36 ATATGCC 50 7.2759576E-12 50.166245 34 CTCCCGC 15 0.008292331 50.16624 34 AAGCGCA 20 4.1442245E-4 50.038265 50 AAGTGGT 40 2.6702764E-9 49.910942 10 >>END_MODULE