FastQCFastQC Report
Fri 17 Jun 2016
SRR1526259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88380
Sequences flagged as poor quality0
Sequence length56
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG30213.4181941615750167No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG7730.8746322697442861No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG7010.7931658746322697No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG6560.7422493776872595No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG2100.23761031907671418No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1650.18669382213170402No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1520.1719846119031455No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC1490.16859017877347815No Hit
TATTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.13238289205702647No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG980.11088481556913328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGTC1000.050.37378738
TCTCTCA150.008179788550.34496740
TTGCCTC204.0237035E-450.34496740
AAACAAC204.0350866E-450.31618541
AAAGGCG150.00823546450.2587136
TATGCTA150.00825408550.23002235
TATGCCT1100.050.2300235
CACGATT355.0977178E-850.1441647
CGATTCA800.050.1441649
ATATGCC2350.050.1441634
ACGATTC800.050.1441648
TTAAGAC150.008310134550.1441634
TCCACGA301.0163658E-650.1441645
CCACGAT301.0163658E-650.1441646
CGATTAA1500.050.14415749
ACGATTA1500.050.14415748
ACAACAA204.1154883E-450.1156143
GATGCGT150.00832888150.11560444
CGTTAAG150.0084041850.00170532
GAAGTTT150.0084041850.00170514