##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526259_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88380 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.00724145734329 38.0 38.0 39.0 35.0 40.0 2 36.81958587915818 38.0 38.0 39.0 33.0 40.0 3 37.564154786150716 38.0 38.0 39.0 35.0 40.0 4 37.738119484046166 38.0 38.0 39.0 35.0 40.0 5 37.087078524553064 38.0 37.0 39.0 33.0 40.0 6 37.35219506675718 39.0 37.0 39.0 33.0 40.0 7 38.05498981670061 39.0 38.0 40.0 35.0 40.0 8 37.90080334917402 39.0 38.0 40.0 35.0 40.0 9 37.87701968771215 39.0 38.0 40.0 35.0 40.0 10 37.14985290789772 39.0 37.0 39.0 33.0 40.0 11 35.44031455080335 38.0 34.0 39.0 28.0 40.0 12 33.9806177868296 38.0 33.0 39.0 22.0 40.0 13 33.73517764200045 38.0 32.0 39.0 21.0 40.0 14 33.71795655125594 38.0 32.0 39.0 20.0 40.0 15 33.634453496266126 38.0 32.0 39.0 20.0 40.0 16 33.48013125141435 38.0 31.0 39.0 20.0 40.0 17 33.51749264539489 38.0 31.0 39.0 21.0 40.0 18 33.01909934374293 37.0 30.0 39.0 19.0 40.0 19 33.736003620728674 37.0 31.0 39.0 23.0 40.0 20 33.817526589726185 37.0 31.0 39.0 23.0 40.0 21 34.809764652636346 38.0 33.0 39.0 26.0 40.0 22 34.57095496718715 38.0 33.0 39.0 25.0 40.0 23 35.124111789997734 38.0 34.0 39.0 27.0 40.0 24 34.60875763747454 38.0 33.0 39.0 25.0 40.0 25 34.830889341479974 38.0 33.0 39.0 26.0 40.0 26 35.60294184204571 38.0 35.0 39.0 29.0 40.0 27 35.54849513464585 38.0 35.0 39.0 29.0 40.0 28 35.29016745870106 38.0 35.0 39.0 28.0 40.0 29 35.21012672550351 38.0 35.0 39.0 27.0 40.0 30 34.949049558723694 38.0 35.0 39.0 27.0 40.0 31 35.406087350079204 38.0 35.0 39.0 29.0 40.0 32 35.08716904276986 38.0 35.0 39.0 27.0 40.0 33 35.27326318171532 38.0 35.0 39.0 28.0 40.0 34 35.25853134193257 38.0 35.0 39.0 29.0 40.0 35 35.048178320887075 38.0 35.0 39.0 28.0 40.0 36 34.443335596288755 38.0 34.0 39.0 28.0 40.0 37 34.18232631817153 37.0 33.0 39.0 27.0 40.0 38 33.91261597646526 37.0 33.0 39.0 26.0 40.0 39 33.428445349626614 36.0 32.0 39.0 23.0 40.0 40 30.11304593799502 35.0 25.0 38.0 9.0 39.0 41 26.720853134193256 32.0 15.0 38.0 5.0 39.0 42 31.04151391717583 35.0 29.0 38.0 12.0 39.0 43 29.48531341932564 34.0 26.0 38.0 6.0 39.0 44 29.565127856981217 34.0 27.0 38.0 5.0 39.0 45 30.715648336727767 35.0 29.0 38.0 4.0 39.0 46 30.284555329260012 35.0 28.0 38.0 4.0 40.0 47 29.835879158180585 35.0 27.0 38.0 4.0 39.0 48 29.935551029644717 35.0 27.0 38.0 4.0 39.0 49 27.415919891378138 34.0 17.0 38.0 4.0 39.0 50 29.456607829825753 35.0 27.0 38.0 4.0 39.0 51 29.714437655578184 35.0 28.0 39.0 4.0 40.0 52 29.563125141434714 35.0 27.0 39.0 4.0 40.0 53 29.12459832541299 35.0 26.0 39.0 4.0 40.0 54 28.131319303009732 35.0 20.0 39.0 4.0 40.0 55 28.46744738628649 35.0 24.0 39.0 4.0 40.0 56 27.23233763294863 34.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.027561031861608853 1 2 1.3446300416950905 1 3 0.9018733742410063 1 4 0.5154291077397986 1 5 0.9291887697936616 1 6 0.4816411179078486 1 7 0.049647315122321345 1 8 0.2772011955939604 1 9 0.2002316456310851 1 10 0.0291939893745905 1 11 -4.577799895860007 1 12 -16.6492287410246 1 13 -11.439511550203672 1 14 -8.176800912894127 1 15 -8.918021435263661 1 16 -23.28548455475514 1 17 -5.3549321361444555 1 18 -12.414792858608468 1 19 -6.28368042886655 1 20 -6.0687553674900805 1 21 -5.764242162810486 1 22 -2.8924942849455455 1 23 -3.207402315226478 1 24 -4.109526352421575 1 25 -3.1702322003949774 1 26 1.498683205988982 1 27 1.6378585074412797 1 28 1.6878066849725855 1 29 2.174619403345467 1 30 2.0590767109818913 1 31 0.9146092527565486 1 32 1.2667809249354107 1 33 1.2441612029263425 1 34 0.43688391687890515 1 35 1.3342277890363903 1 36 1.2623468158693711 1 37 0.9943885220191504 1 38 1.3010245894904742 1 39 1.4200567937598336 1 40 4.192261357518348 1 41 -10.751610301452313 1 42 -11.557668847226822 1 43 -18.70259713715234 1 44 -22.812575619456368 1 45 -13.895896604057183 1 46 -18.916540388581964 1 47 -23.439028319140377 1 48 -17.075180496818497 1 49 -20.21642871973842 1 50 -16.347321061879107 1 51 -14.337228359177768 1 52 -13.993648144024691 1 53 -13.677529694016911 1 54 -12.672887796309043 1 55 -13.232873671493758 1 56 -9.860702548461639 2 1 0.7757823757351261 2 2 1.7193335990073493 2 3 1.2860630975611684 2 4 1.0395397796765593 2 5 1.310611694694849 2 6 1.252392106050138 2 7 0.9698054178891198 2 8 0.9064502074516625 2 9 0.8583344124294996 2 10 1.196387665263913 2 11 0.6162712503850543 2 12 -2.6274896105898193 2 13 -1.6391162932866763 2 14 2.9465192452086413 2 15 2.4685398295584733 2 16 2.7338830341776656 2 17 3.583803041721154 2 18 -0.7487849534701283 2 19 2.2566357766670464 2 20 2.2569363321146625 2 21 2.9270621850156004 2 22 3.170746821773818 2 23 2.815522585959293 2 24 2.007469695009256 2 25 2.964550408300674 2 26 2.6121219411668477 2 27 2.437067993607286 2 28 2.75934818694887 2 29 2.8184929211320267 2 30 2.675677501495727 2 31 2.2063088574996357 2 32 2.6758718340263243 2 33 1.6789438116219912 2 34 2.1483463674717953 2 35 1.9128839155186057 2 36 2.406615590572933 2 37 2.8319379291337725 2 38 2.619996921506285 2 39 3.37776430364125 2 40 6.015581515621108 2 41 -4.24093836469342 2 42 -5.837906001377018 2 43 -11.62156946916815 2 44 -14.103879967282454 2 45 -5.125145615914892 2 46 -9.934326949846788 2 47 -18.634285236136428 2 48 -7.5767615244864785 2 49 -12.20536152606253 2 50 -6.916886279270415 2 51 -1.06489634336749 2 52 -0.9640038752499862 2 53 -0.8846443185228381 2 54 -0.09186012832485346 2 55 -0.3178539086479084 2 56 2.7744753171510084 3 1 0.7248409173304751 3 2 1.7549378441288823 3 3 1.5130401602109416 3 4 0.9636753496868309 3 5 1.2782939952768402 3 6 1.2136382546293945 3 7 0.9219450960815223 3 8 1.0900859486361938 3 9 1.0137606363254292 3 10 1.361262708057481 3 11 2.372450641004704 3 12 3.9846295266919327 3 13 2.7176108549502302 3 14 3.343027290398645 3 15 3.0736750572202283 3 16 3.9820172347938936 3 17 4.522591127992982 3 18 5.13173541626626 3 19 4.033356077933732 3 20 3.71506710924578 3 21 3.2507184671107723 3 22 3.7163818782613234 3 23 3.420657813621048 3 24 3.704217384156806 3 25 2.843153626376676 3 26 2.202262304056269 3 27 2.314164878235836 3 28 2.120662805230282 3 29 2.3002457455072403 3 30 2.6361704833512505 3 31 1.3733064610600607 3 32 2.1294151411129363 3 33 2.0709328564696463 3 34 0.688709256893226 3 35 2.0338698792296626 3 36 2.5348593427126076 3 37 2.93833984628543 3 38 3.128726808531276 3 39 3.0615027493790166 3 40 6.391617119866233 3 41 6.7103796770730995 3 42 3.6897899931454035 3 43 -1.2106143168230616 3 44 2.074825955346782 3 45 3.8455493515620027 3 46 1.8948411432720071 3 47 -2.370882291939232 3 48 2.4057787014635394 3 49 4.622368703310972 3 50 2.820395473553962 3 51 3.4051481619389143 3 52 3.527540116533647 3 53 3.8171016588821587 3 54 4.812008409847007 3 55 4.321242291283621 3 56 4.420829305511159 4 1 1.0486177529183323 4 2 1.9508460479110994 4 3 1.7672968396644748 4 4 1.3733933657040538 4 5 1.7292664698713622 4 6 1.333700169966896 4 7 1.1363606018356052 4 8 1.1704412888340485 4 9 1.276766222165655 4 10 1.787469047649644 4 11 3.1063492882891737 4 12 4.893506301710445 4 13 4.986673375981255 4 14 4.598024261931052 4 15 4.76605004880782 4 16 5.462534093263507 4 17 5.230848749636429 4 18 5.228175284359672 4 19 4.2717974266113075 4 20 4.0477947490600386 4 21 3.5425954440271212 4 22 3.7637605713093123 4 23 3.660041352217185 4 24 4.483946841051619 4 25 3.425494295331518 4 26 2.936833944139721 4 27 2.2965997661825384 4 28 2.512748409914316 4 29 2.4640521927782544 4 30 2.652860704765885 4 31 1.847631785779086 4 32 2.8861282442827303 4 33 2.415101373866513 4 34 2.5509476309426233 4 35 1.8621221169307205 4 36 2.847195300717857 4 37 3.569135996766626 4 38 3.794653183119067 4 39 4.0883551620582 4 40 7.755975421232407 4 41 9.797263047421033 4 42 5.518244128686156 4 43 7.0396717051165005 4 44 6.210501303620553 4 45 5.137359029198446 4 46 5.289753317711739 4 47 5.2580967779847185 4 48 5.091019969381971 4 49 7.595381692071989 4 50 5.638149023590977 4 51 4.249484927535516 4 52 4.453982003605457 4 53 5.129774113286896 4 54 5.266816943395696 4 55 4.88608514746506 4 56 5.452661864902776 5 1 0.8243712391981362 5 2 1.8066715089997203 5 3 1.5698159579730628 5 4 0.9822552640396367 5 5 1.0826177969069022 5 6 1.1229489328998739 5 7 0.6080205208639455 5 8 0.7271214507773678 5 9 0.8015075626964716 5 10 0.748332745355448 5 11 2.408643485320219 5 12 4.49989406599294 5 13 4.013439008010046 5 14 4.2350013199607375 5 15 4.8377999363471815 5 16 4.754706832804665 5 17 3.7956260775716544 5 18 4.85952931045054 5 19 3.669908087879861 5 20 3.934434425173393 5 21 3.2778631003474104 5 22 3.326963450301662 5 23 2.7549581313444946 5 24 3.328751159540147 5 25 2.7894169224120446 5 26 1.7832127115711174 5 27 2.324939847154198 5 28 1.6051910549885378 5 29 1.7416050492784763 5 30 2.07773699806323 5 31 1.144752793426406 5 32 1.5460472726228147 5 33 1.6652138345052876 5 34 1.4812220349012364 5 35 1.7152485226887038 5 36 3.101581265630138 5 37 3.1334634821467375 5 38 2.7759847170821814 5 39 3.4385575912079958 5 40 7.819853064057419 5 41 8.721914419440829 5 42 5.2337187125817906 5 43 6.64875852472964 5 44 6.21724894194714 5 45 5.205060333741859 5 46 5.718228351449934 5 47 5.333220484687374 5 48 4.716526042256465 5 49 7.874958840070192 5 50 5.271977183734926 5 51 5.080793969370877 5 52 5.128520914986471 5 53 4.441290082697602 5 54 5.77671347960802 5 55 4.796791400276575 5 56 5.07119537817313 6 1 0.5130747051801592 6 2 1.177446006218375 6 3 1.3203508317428572 6 4 0.9114379769194016 6 5 1.0905339286997986 6 6 0.8056618126602473 6 7 0.6116576624985228 6 8 0.7320422894594429 6 9 0.726839184433743 6 10 0.6486322746665323 6 11 2.871202321434893 6 12 3.88329158417865 6 13 4.059010253196178 6 14 4.132733455176826 6 15 4.218416110929979 6 16 4.506311454114041 6 17 4.48475744256951 6 18 4.448104406727822 6 19 3.7858687211704023 6 20 3.2497959998417727 6 21 3.2203106827179653 6 22 2.996197511063322 6 23 2.6466996803389335 6 24 3.3000819254793896 6 25 3.1574100760277872 6 26 1.563058667925418 6 27 1.780652299015557 6 28 2.3332612304271336 6 29 2.54305990593231 6 30 1.8083745675968217 6 31 1.3537814626604714 6 32 1.638473460042583 6 33 0.2604213655279679 6 34 1.6070243455559208 6 35 0.5652699176394975 6 36 1.700853837303228 6 37 2.4255045604522607 6 38 1.9105589575614275 6 39 2.7969230169675185 6 40 6.125372961361656 6 41 2.888153187091664 6 42 4.126957908131644 6 43 5.780551421605974 6 44 4.637017876478595 6 45 3.3777175866301796 6 46 2.3410353689937935 6 47 3.1757536468611036 6 48 3.02097619202112 6 49 4.597466328302971 6 50 2.8860122714542236 6 51 1.968684427222822 6 52 1.878413940232516 6 53 2.0238745927532307 6 54 2.256602225863908 6 55 2.4906296544485933 6 56 2.533754214287807 7 1 0.7056784896250861 7 2 1.8247587625317792 7 3 1.0798299309586241 7 4 0.8885586330817148 7 5 1.2842169274766917 7 6 1.0620111903133136 7 7 0.8681509353958532 7 8 1.0667426276132659 7 9 0.8675687333221518 7 10 1.0091618091654126 7 11 3.0557479722011323 7 12 4.965815506763008 7 13 4.394358119949182 7 14 4.732129095150924 7 15 4.753418629096757 7 16 4.581208928752503 7 17 5.081994653879676 7 18 5.363565950723789 7 19 3.7630613249548475 7 20 4.163746643772988 7 21 2.56407642126031 7 22 3.185060019157433 7 23 3.082702270453325 7 24 3.45217919866451 7 25 3.080210277481129 7 26 2.35556172569936 7 27 2.9099421759851225 7 28 2.4925532658253644 7 29 2.4421173920823946 7 30 2.2833727689062684 7 31 1.4095408698120622 7 32 1.9802966127873844 7 33 1.713187751598909 7 34 1.2359185106037671 7 35 1.6645576361803975 7 36 1.5433604924582696 7 37 1.643865593620113 7 38 2.0763262789514556 7 39 3.199541910413089 7 40 5.549863931935079 7 41 -0.43093451705971475 7 42 3.4039562533363323 7 43 5.410596748607919 7 44 4.880344734525934 7 45 4.018986742684572 7 46 4.49737755188465 7 47 4.9107705545523 7 48 4.4663319730125615 7 49 6.531922044541545 7 50 4.393386902722661 7 51 3.7334876504762953 7 52 4.054541719209421 7 53 4.5960020839396485 7 54 5.227031753168028 7 55 3.9223153872351233 7 56 5.063432608416882 8 1 0.6469083512089284 8 2 1.3867434838085373 8 3 1.365587437955071 8 4 0.8263848186955087 8 5 0.9856767262816177 8 6 0.82942666569339 8 7 0.9825144479894519 8 8 0.7687318871246518 8 9 0.9177918631912974 8 10 1.1891784493590478 8 11 2.8755800575199473 8 12 4.491796900001042 8 13 3.9695793588872377 8 14 4.5467422106490005 8 15 4.774597057354832 8 16 4.881337512066928 8 17 4.675293194080872 8 18 5.134158190342582 8 19 4.066669221483096 8 20 4.107623808889095 8 21 3.2178091192407976 8 22 3.182563990112726 8 23 3.172861865037703 8 24 3.7489041060088866 8 25 2.5707934406306663 8 26 1.8086288159345898 8 27 2.185488655071424 8 28 1.8033467005126056 8 29 2.0708897996158626 8 30 2.097305149210328 8 31 1.4459223840696822 8 32 1.8092051673596572 8 33 1.2441612029263425 8 34 1.4996655796605722 8 35 1.2980195528281513 8 36 0.7189901725127328 8 37 0.9879394155700396 8 38 1.1279865164524026 8 39 2.242201315904353 8 40 4.516659181916168 8 41 -0.5788053286473414 8 42 3.38149199193402 8 43 5.219158884603683 8 44 5.142125235244485 8 45 3.992059883899305 8 46 4.546163574121994 8 47 5.01878053866848 8 48 4.185037063399069 8 49 7.313330410020711 8 50 4.740713126155079 8 51 3.770852448903039 8 52 3.5309050805285374 8 53 3.001568985081768 8 54 4.1557058322845855 8 55 3.3305295919095066 8 56 2.538131950372861 9 1 0.5159312202317992 9 2 1.204253301318353 9 3 1.3616604340280674 9 4 0.8672718595825515 9 5 0.5001142407191352 9 6 1.099003935270666 9 7 0.735806201281207 9 8 0.6922708106635724 9 9 0.597856543255979 9 10 0.7341079942885926 9 11 2.4570076389475304 9 12 4.075996484200623 9 13 4.158768548076424 9 14 4.320823984730772 9 15 4.480995763753533 9 16 4.450060080789498 9 17 4.120661639449317 9 18 4.849334905519292 9 19 4.017466172280052 9 20 3.7789104801757674 9 21 2.6024245038561773 9 22 3.019708827257567 9 23 3.3144650066408445 9 24 3.1471485042532805 9 25 2.6414795113167386 9 26 2.134801142106916 9 27 1.5980550676378371 9 28 1.9458738430397489 9 29 1.7999265286525912 9 30 2.1652192171243954 9 31 0.9624389005862 9 32 1.7371330952875823 9 33 1.5594765182416594 9 34 1.1014211814161712 9 35 1.9298051846137838 9 36 1.9643124178349751 9 37 2.0255924532230765 9 38 2.909460297926188 9 39 2.9428250165280545 9 40 6.076372741629726 9 41 3.3361865863445743 9 42 3.1680477784898073 9 43 4.984462649907446 9 44 5.3594964526157085 9 45 3.4205653124047295 9 46 4.611305639264064 9 47 3.9709634908514317 9 48 4.114581992943993 9 49 6.313792410482712 9 50 4.534660920102873 9 51 3.9750566531072415 9 52 3.479161028784482 9 53 3.526401509914294 9 54 3.124982801561554 9 55 2.621589882969797 9 56 2.7591991714400805 10 1 0.7410767197135826 10 2 1.4619269041247023 10 3 1.1329272760913724 10 4 0.8354612879489736 10 5 0.83288949384837 10 6 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0.6008820285126575 114 38 1.385007273473164 114 39 1.9473295210325574 114 40 6.03100594626293 114 41 6.398606148764138 114 42 1.4492575596995856 114 43 -2.2841988687540713 114 44 -8.926861333742082 114 45 0.8623285041679232 114 46 -6.254202726244301 114 47 -5.084699314811374 114 48 -6.725829847467846 114 49 -18.20712136043106 114 50 -5.673511538069583 114 51 -5.590258662208072 114 52 -5.166591866968414 114 53 -6.660213676700895 114 54 -9.240203813625058 114 55 -4.899323887943975 114 56 -1.0992306869897774 115 1 -0.019445620023091692 115 2 1.3603728354866647 115 3 0.35287115694610804 115 4 0.16753553789500586 115 5 0.8628916522770353 115 6 0.5820845768878868 115 7 0.16450319095380905 115 8 0.288952858565132 115 9 0.2878147941898419 115 10 0.6283070714144969 115 11 -2.5556269468577923 115 12 -10.612643375170943 115 13 -7.469445031356663 115 14 -11.062388496042683 115 15 -10.273453807769204 115 16 -9.042469033690839 115 17 -2.454266947674391 115 18 -2.8405134794510403 115 19 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0.5962903517589382 118 5 0.9992844635735665 118 6 0.666808582022675 118 7 0.2527573629692128 118 8 0.4420337314791283 118 9 0.24449001883682087 118 10 0.7878064295659755 118 11 -3.5431109006446952 118 12 -7.938702425235128 118 13 -8.372525904270656 118 14 -14.216753066004173 118 15 -8.653428133828257 118 16 -14.126872114563753 118 17 -11.814023045235366 118 18 -11.320295250952967 118 19 -5.330091912120139 118 20 -5.425214697633621 118 21 0.14628415297898556 118 22 0.848893274863066 118 23 0.2330283067830905 118 24 0.8550669490138318 118 25 0.7718730627629213 118 26 1.8577741150798914 118 27 1.8775714261015608 118 28 1.3201033356902911 118 29 1.9652892598047913 118 30 2.1435264717474425 118 31 0.5394896355316732 118 32 -4.20833869228947 118 33 0.8099506766105549 118 34 0.9110465963047432 118 35 0.7064765928641421 118 36 1.4831602129985626 118 37 1.2869722540765665 118 38 2.482125067959373 118 39 2.934171626295715 118 40 5.591782888618823 118 41 2.743844244002233 118 42 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43 -10.826057547709524 120 44 -7.58354336139185 120 45 -5.3008819413005845 120 46 -13.16434097509223 120 47 -11.494453539081729 120 48 -10.995415100924067 120 49 -17.37801229745103 120 50 -9.9957081586533 120 51 -8.383269414896244 120 52 -8.302152542851669 120 53 -9.347617670556499 120 54 -10.738283690737195 120 55 -8.806773964748889 120 56 -6.69677292969331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 240.0 5 17.0 6 51.0 7 109.0 8 235.0 9 219.0 10 112.0 11 108.0 12 87.0 13 92.0 14 157.0 15 171.0 16 237.0 17 311.0 18 406.0 19 486.0 20 594.0 21 754.0 22 1006.0 23 1223.0 24 1458.0 25 1680.0 26 1739.0 27 1994.0 28 2349.0 29 2861.0 30 3315.0 31 4090.0 32 4543.0 33 5253.0 34 6000.0 35 7347.0 36 9483.0 37 11959.0 38 11294.0 39 6390.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.42860375650599 0.3790450328128536 0.12333107037791355 0.06902014030323603 8 98.60149355057706 1.1371350984385609 0.14709210228558497 0.11427924869880063 9 93.83910386965377 4.026929169495361 1.2186014935505771 0.9153654673002942 10 33.17945236478842 38.334464811043226 19.685449196650826 8.800633627517538 11 26.800181036433585 39.57909029192125 18.713509843856077 14.907218827789093 12 32.62502828694275 16.246888436297805 18.96356641774157 32.16451685901788 13 22.643324355521354 16.29472874182378 23.32118688183238 37.74076002082249 14 23.2840752223404 20.987123492271834 32.592952997352285 23.135848288035486 15 34.51013302788709 17.856130283407236 24.06863467797725 23.565102010728424 16 35.19755462470282 21.168345975319824 22.92086493829956 20.7132344616778 17 25.72301425661914 21.760579316587464 31.32948630911971 21.186920117673683 18 21.864922662623474 23.17858314758031 32.955792665678494 22.00070152411772 19 34.0664567713878 24.007338784570432 20.84078914584702 21.08541529819475 20 22.583787334775568 33.62819830329939 21.751294045690855 22.036720316234184 21 22.664630006788865 22.551482235799956 33.475899524779365 21.307988232631818 22 22.638606019461417 21.8816474315456 24.396922380629103 31.08282416836388 23 23.317492645394886 23.33785924417289 24.4908350305499 28.85381307988233 24 23.0663045937995 21.96990269291695 35.52387417967866 19.43991853360489 25 33.478541282431344 21.55376277169916 26.042385634596453 18.925310311273037 26 23.01103253182461 21.043281471004242 27.404809052333807 28.54087694483734 27 21.816678746149666 19.70012683457148 29.307845624207285 29.17534879507157 28 21.959425017840758 30.96362750761772 29.778774594759916 17.298172879781607 29 30.71168254974308 21.276908798696155 30.721868845779476 17.28953980578129 30 20.40763206735775 20.362364763931012 42.96885609524241 16.261147073468834 31 17.863084392435578 31.34074987833999 35.45002885888571 15.346136870338725 32 27.765699662567656 20.748692166587404 36.280770885703284 15.204837285141654 33 17.356327048585932 21.04786076867295 37.58951232777375 24.006299854967367 34 15.245296687872134 31.382581331642932 40.029657467569216 13.342464512915713 35 15.265576888244883 21.997826480710017 50.563756565839526 12.172840065205579 36 15.450779782249485 31.75716969600942 39.54480635596098 13.247244165780122 37 15.848350901054062 21.948316899013943 49.449166950017 12.754165249914994 38 25.603393521459033 22.429906542056074 39.407721622357315 12.558978314127575 39 16.00947802820734 21.895832388553806 38.99369643100086 23.100993152237994 40 12.391609029067581 17.25481705996969 42.8516083453926 27.50196556557013 41 11.669822728206064 16.00693573041911 40.60596381556433 31.7172777258105 42 23.461377255683814 21.836945070629948 40.202997383236855 14.49868029044938 43 12.423036364254855 18.941842138086653 48.006032361578846 20.629089136079646 44 13.089879978177851 19.760410983815238 38.444489907255864 28.705219130751047 45 14.672440569984015 22.603244419756724 49.099339099679185 13.624975910580073 46 14.048050770625567 22.74365367180417 39.95806890299184 23.250226654578423 47 13.917846672778927 30.282861896838604 40.231134050958154 15.568157379424315 48 15.251778996509993 31.698998322984185 39.63536237139101 13.413860309114808 49 12.439027163469737 25.5420755210406 35.41256864773903 26.606328667750628 50 22.92650874032786 23.564331758601547 40.11034451506191 13.398814986008677 51 14.401279811205155 32.30502167056207 40.025868524359524 13.267829993873246 52 14.511598405219283 23.64647517216383 47.27482783617253 14.567098586444363 53 14.269526690915685 23.764036668139738 47.79549240235238 14.170944238592199 54 13.498866213151928 30.763038548752835 36.986394557823125 18.75170068027211 55 13.80174378394313 33.41194344607082 38.80089343416592 13.985419335820135 56 23.815190045556538 24.24582398404388 37.50368305342127 14.43530291697831 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 5.0 5 9.0 6 43.0 7 77.0 8 168.5 9 260.0 10 323.5 11 387.0 12 446.5 13 622.0 14 738.0 15 895.0 16 1052.0 17 1195.0 18 1338.0 19 1633.5 20 1929.0 21 2335.0 22 3185.0 23 3629.0 24 4093.5 25 4558.0 26 4655.0 27 4752.0 28 4446.0 29 3788.5 30 3437.0 31 2907.0 32 2377.0 33 2120.5 34 1864.0 35 1745.0 36 1626.0 37 1653.5 38 1726.0 39 1771.0 40 1892.5 41 2014.0 42 3886.5 43 5759.0 44 5539.0 45 5319.0 46 4407.5 47 3282.0 48 3068.0 49 3228.0 50 3388.0 51 3353.5 52 3319.0 53 3370.0 54 3365.0 55 3309.0 56 3376.0 57 3443.0 58 3255.0 59 3067.0 60 2803.0 61 2539.0 62 2315.0 63 1848.5 64 1606.0 65 1409.5 66 1213.0 67 1057.0 68 901.0 69 746.0 70 591.0 71 515.0 72 351.5 73 264.0 74 202.0 75 140.0 76 113.5 77 87.0 78 54.0 79 14.0 80 7.0 81 5.5 82 4.0 83 2.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01584068793844761 14 0.0022629554197782305 15 0.2296899751074904 16 0.056573885494455765 17 0.0 18 0.0011314777098891152 19 0.09278117221090744 20 0.10296447159990949 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0011314777098891152 26 0.005657388549445576 27 0.08599230595157276 28 0.1120162932790224 29 0.029418420457116998 30 0.018103643358225844 31 0.02149807648789319 32 0.0746775288526816 33 0.14030323602625028 34 0.04299615297578638 35 0.04978501923512107 36 0.024892509617560534 37 0.1697216564833673 38 0.23987327449649243 39 0.19914007694048427 40 0.7003847024213623 41 0.8124009957003847 42 0.11654220411857887 43 0.21384928716904278 44 0.4480651731160896 45 0.1889567775514822 46 0.15840687938447612 47 0.0373387644263408 48 0.14482914686580675 49 0.48766689296220866 50 0.1255940257976918 51 0.2738176057931659 52 0.1040959493097986 53 0.14596062457569586 54 0.20366598778004072 55 0.20479746548992986 56 0.1561439239646979 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 88380.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.63928490608735 #Duplication Level Percentage of deduplicated Percentage of total 1 86.80463231407474 61.31817153202081 2 7.608399673239257 10.749038243946595 3 2.4266790536752576 5.142566191446028 4 1.1148307731735836 3.1500339443312964 5 0.6198843523249667 2.189409368635438 6 0.38282263619035417 1.622539035980991 7 0.23545994778235171 1.1642905634758995 8 0.16978744533965498 0.9594930979859697 9 0.12974323653313258 0.8248472505091651 >10 0.4757252006214861 5.362072867164517 >50 0.017619451874869855 0.7920343969223806 >100 0.008008841761304479 0.8972618239420683 >500 0.004805305056782688 2.4100475220638153 >1k 0.0016017683522608962 3.4181941615750167 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 3021 3.4181941615750167 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 773 0.8746322697442861 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 701 0.7931658746322697 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 656 0.7422493776872595 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 210 0.23761031907671418 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 165 0.18669382213170402 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 152 0.1719846119031455 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 149 0.16859017877347815 No Hit TATTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.13238289205702647 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG 98 0.11088481556913328 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0011314777098891152 0.0 0.0 0.0 0.0 9 0.0022629554197782305 0.0 0.0 0.0 0.0 10 0.014709210228558497 0.0 0.0 0.0 0.0 11 0.022629554197782304 0.0 0.0 0.0 0.0 12 0.02602398732744965 0.0 0.0 0.0 0.0 13 0.029418420457116994 0.0 0.0 0.0 0.0 14 0.03620728671645169 0.0 0.0 0.0 0.0 15 0.04186467526589726 0.0 0.0 0.0 0.0 16 0.05091649694501019 0.0 0.0 0.0 0.0 17 0.05770536320434488 0.0 0.0 0.0 0.0 18 0.07015161801312514 0.0 0.0 0.0 0.0 19 0.08033491740212718 0.0 0.0 0.0 0.0 20 0.08712378366146187 0.0 0.0 0.0 0.0 21 0.09164969450101833 0.0 0.0 0.0 0.0 22 0.10070151618013125 0.0 0.0 0.0 0.0 23 0.10862186014935506 0.0 0.0 0.0 0.0 24 0.11767368182846798 0.0 0.0 0.0 0.0 25 0.13238289205702647 0.0 0.0 0.0 0.0 26 0.14369766915591764 0.0 0.0 0.0 0.0 27 0.1561439239646979 0.0 0.0 0.0 0.0 28 0.17651052274270196 0.0 0.0 0.0 0.0 29 0.18329938900203666 0.0 0.0 0.0 0.0 30 0.18895677755148224 0.0 0.0 0.0 0.0 31 0.1934826883910387 0.0 0.0 0.0 0.0 32 0.19914007694048427 0.0 0.0 0.0 0.0 33 0.20027155465037338 0.0 0.0 0.0 0.0 34 0.20366598778004075 0.0 0.0 0.0 0.0 35 0.2081918986195972 0.0 0.0 0.0 0.0 36 0.2093233763294863 0.0 0.0 0.0 0.0 37 0.2172437202987101 0.0 0.0 0.0 0.0 38 0.22063815342837745 0.0 0.0 0.0 0.0 39 0.2217696311382666 0.0 0.0 0.0 0.0 40 0.2240325865580448 0.0 0.0 0.0 0.0 41 0.22855849739760126 0.0 0.0 0.0 0.0 42 0.23647884136682507 0.0 0.0 0.0 0.0 43 0.24553066304593799 0.0 0.0 0.0 0.0 44 0.2545824847250509 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTGTC 100 0.0 50.373787 38 TCTCTCA 15 0.0081797885 50.344967 40 TTGCCTC 20 4.0237035E-4 50.344967 40 AAACAAC 20 4.0350866E-4 50.316185 41 AAAGGCG 15 0.008235464 50.25871 36 TATGCTA 15 0.008254085 50.230022 35 TATGCCT 110 0.0 50.23002 35 CACGATT 35 5.0977178E-8 50.14416 47 CGATTCA 80 0.0 50.14416 49 ATATGCC 235 0.0 50.14416 34 ACGATTC 80 0.0 50.14416 48 TTAAGAC 15 0.0083101345 50.14416 34 TCCACGA 30 1.0163658E-6 50.14416 45 CCACGAT 30 1.0163658E-6 50.14416 46 CGATTAA 150 0.0 50.144157 49 ACGATTA 150 0.0 50.144157 48 ACAACAA 20 4.1154883E-4 50.11561 43 GATGCGT 15 0.008328881 50.115604 44 CGTTAAG 15 0.00840418 50.001705 32 GAAGTTT 15 0.00840418 50.001705 14 >>END_MODULE