FastQCFastQC Report
Fri 17 Jun 2016
SRR1526258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27346
Sequences flagged as poor quality0
Sequence length56
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1120.40956629854457693No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG550.2011263073209976No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.16090104585679807No Hit
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG370.135303152197762No Hit
GCAGGAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA360.13164631024647117No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG360.13164631024647117No Hit
GCAGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.1279894682951803No Hit
GCAGGAGGGAATGTTAATTTAACTAACTTGTCTTTTTTAGCCTGTGCTTAATTTAA340.1243326263438894No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG330.12067578439259855No Hit
GCAGGAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA330.12067578439259855No Hit
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG320.11701894244130769No Hit
GCAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA290.1060484165874351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACA203.8591743E-450.6436639
ACGCCCA150.00793072650.64365839
CGCCCAG150.00793072650.64365840
TACGCCC150.00793072650.64365838
TACCCAC150.00793072650.64365838
ACACCGC150.00798953850.54934737
AGCCCTG150.00798953850.54934737
CCTGCAC150.00804867450.4553936
GCGGCCA150.00822803850.17560244
TCTCAAC204.0407866E-450.17560243
CCCAGTT150.00828848250.08302742
CAGCAAA204.0779103E-450.08302746
AACGATT204.0779103E-450.08302747
GTTCTGA354.971662E-850.08302746
GCAGCAA150.00828848250.08302745
GGCCAAG204.0779103E-450.08302746
ACGATTA204.0779103E-450.08302748
CGGCCAA204.0779103E-450.08302745
CAAGGAG150.00828848250.08302749
AATGTTT150.00828848250.08302749