##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526258_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27346 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.59105536458714 39.0 38.0 39.0 34.0 40.0 2 37.24182695823887 39.0 38.0 39.0 33.0 40.0 3 36.094565932860384 39.0 36.0 39.0 30.0 40.0 4 37.04037153514225 39.0 37.0 39.0 33.0 40.0 5 37.17205441380823 39.0 38.0 39.0 33.0 40.0 6 35.22021502230674 38.0 35.0 39.0 23.0 40.0 7 37.09006801726029 39.0 37.0 39.0 33.0 40.0 8 37.09789365903605 39.0 38.0 39.0 33.0 40.0 9 36.22025159072625 39.0 35.0 39.0 30.0 40.0 10 35.84666861698238 39.0 35.0 39.0 29.0 40.0 11 34.89731587800775 38.0 33.0 39.0 27.0 40.0 12 33.61504424778761 38.0 32.0 39.0 20.0 40.0 13 33.373436700065824 38.0 31.0 39.0 20.0 40.0 14 33.61493454252907 38.0 32.0 39.0 20.0 40.0 15 33.617860016090106 38.0 32.0 39.0 20.0 40.0 16 33.44372120236963 38.0 32.0 39.0 21.0 40.0 17 33.41263804578366 37.0 31.0 39.0 21.0 40.0 18 32.83295545966504 37.0 30.0 39.0 18.0 40.0 19 33.74943318949755 37.0 31.0 39.0 23.0 40.0 20 33.78885394573246 37.0 31.0 39.0 23.0 40.0 21 34.558363197542604 38.0 33.0 39.0 25.0 40.0 22 34.223213632706795 38.0 33.0 39.0 23.0 40.0 23 34.6099246690558 38.0 33.0 39.0 26.0 40.0 24 34.18134279236451 38.0 33.0 39.0 23.0 40.0 25 34.49974402106341 38.0 33.0 39.0 25.0 40.0 26 35.11029035325093 38.0 35.0 39.0 27.0 40.0 27 34.93765084473049 38.0 34.0 39.0 27.0 40.0 28 34.90232575148102 38.0 34.0 39.0 27.0 40.0 29 34.7696920939077 38.0 34.0 39.0 26.0 40.0 30 34.67289548745703 38.0 34.0 39.0 26.0 40.0 31 35.17344401374972 38.0 35.0 39.0 28.0 40.0 32 34.897608425363856 38.0 35.0 39.0 27.0 40.0 33 35.0436261244789 38.0 35.0 39.0 28.0 40.0 34 35.08480216485044 38.0 35.0 39.0 28.0 40.0 35 34.89837636217363 38.0 35.0 39.0 27.0 40.0 36 34.356834637606966 37.0 33.0 39.0 28.0 39.0 37 34.02515907262488 37.0 33.0 39.0 27.0 39.0 38 33.61625100563154 36.0 32.0 39.0 25.0 39.0 39 33.209061654355295 36.0 32.0 39.0 23.0 39.0 40 29.879726468222042 35.0 25.0 38.0 9.0 39.0 41 26.296131061215533 31.0 15.0 38.0 5.0 39.0 42 30.890660425656403 35.0 29.0 38.0 11.0 39.0 43 29.43249469757917 34.0 27.0 38.0 5.0 39.0 44 29.285709061654355 34.0 26.0 38.0 4.0 39.0 45 30.44064945513055 35.0 29.0 38.0 4.0 39.0 46 30.03141227236159 35.0 27.0 38.0 4.0 39.0 47 29.51371315731734 35.0 27.0 38.0 4.0 39.0 48 29.60348862722153 35.0 27.0 38.0 4.0 39.0 49 26.958714254369927 33.0 15.0 38.0 4.0 39.0 50 29.1577927301982 35.0 26.0 38.0 4.0 39.0 51 29.335368975352885 35.0 27.0 39.0 4.0 40.0 52 29.209354201711403 35.0 26.0 39.0 4.0 40.0 53 28.748226431653624 35.0 25.0 39.0 4.0 39.0 54 27.714144664667593 35.0 17.0 39.0 4.0 39.0 55 28.032180209171358 35.0 22.0 39.0 4.0 39.0 56 26.85782198493381 34.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.867210841233053 1 2 1.3807024198154991 1 3 2.9181119821802 1 4 1.461661257168494 1 5 0.8736328946631389 1 6 3.9779030581388426 1 7 1.2650458239940363 1 8 1.5169533782466047 1 9 2.06008611345532 1 10 2.1410701541277533 1 11 -3.5025647913460034 1 12 -13.81961135710835 1 13 -12.652930848328694 1 14 -6.531105332089155 1 15 -6.584869786011218 1 16 -24.061266157516357 1 17 -4.760866617290077 1 18 -14.05175617550833 1 19 -5.751584953161107 1 20 -5.120430275776798 1 21 -7.254042685553706 1 22 -3.522414898069549 1 23 -3.994817646437788 1 24 -4.591285559675249 1 25 -4.020131324452301 1 26 2.495892263631454 1 27 3.2783542519242346 1 28 2.610852481510001 1 29 3.258958230225268 1 30 3.1653427412466186 1 31 2.211050799930163 1 32 3.041356621501727 1 33 2.8409334898945033 1 34 2.5530428504176115 1 35 2.888938740597517 1 36 3.2752018993037666 1 37 3.9255868105281166 1 38 4.450043589997577 1 39 4.959232197801953 1 40 8.504575604202259 1 41 -5.459993438075966 1 42 -5.241060752368583 1 43 -15.437278319595695 1 44 -21.26363693652372 1 45 -7.753474682164811 1 46 -13.336527396447355 1 47 -22.2695425058532 1 48 -9.514155977014305 1 49 -18.693904693712174 1 50 -9.27706595536636 1 51 -5.4859041932160615 1 52 -5.523950520419596 1 53 -5.431340796470277 1 54 -4.194395251642501 1 55 -4.823003406855211 1 56 0.48685111838769046 2 1 1.2043818938646353 2 2 1.5961629461312867 2 3 3.2322567190223026 2 4 1.950148099273754 2 5 1.6894223683473513 2 6 3.6966530581388426 2 7 2.209124771362454 2 8 2.3524796940360773 2 9 2.9745597976658473 2 10 2.8219912067593356 2 11 1.808290471811894 2 12 -2.6123745150030864 2 13 -0.9177334799076426 2 14 3.3274472994897906 2 15 2.273682845567727 2 16 1.6870891056415402 2 17 2.4874886458678205 2 18 -0.8379403860346457 2 19 0.7220992573652083 2 20 1.5308855136968873 2 21 3.244312577604191 2 22 2.9117956282462387 2 23 1.6071560377727394 2 24 1.7985170719036958 2 25 2.438750254495069 2 26 1.8001685794209266 2 27 1.7043410940294947 2 28 1.5516419551942136 2 29 1.451392440751583 2 30 1.9202769517729337 2 31 1.8097350104564782 2 32 1.3883960951859393 2 33 1.597512437262921 2 34 1.4921875872597141 2 35 2.059991372176462 2 36 3.069609794040609 2 37 3.7792052315807467 2 38 4.157280432102837 2 39 4.5332453556966925 2 40 7.5325361305180465 2 41 -0.06525659597070188 2 42 -2.9121133839475313 2 43 -7.468528319595695 2 44 -11.36067641020793 2 45 -0.5281457347963894 2 46 -7.861198449078934 2 47 -17.58862145322162 2 48 -6.3250112401721985 2 49 -10.328773114764806 2 50 -6.020487007997939 2 51 -1.3099173511107978 2 52 -1.4499373625248602 2 53 -0.9823276385755406 2 54 0.34178895888381433 2 55 0.16383869840794674 2 56 4.7154695394403205 3 1 1.4359608412330545 3 2 1.986952419815502 3 3 3.749361982180197 3 4 2.0804112571684925 3 5 2.373632894663139 3 6 4.071653058138843 3 7 2.4525458239940363 3 8 2.5419533782466033 3 9 2.2100861134553185 3 10 2.6785701541277547 3 11 2.741185208653995 3 12 4.942888642891653 3 13 3.703319151671309 3 14 4.356394667910848 3 15 3.477630213988782 3 16 4.969983842483643 3 17 5.045383382709922 3 18 4.860743824491671 3 19 4.348415046838895 3 20 3.8795697242232023 3 21 3.0522073144462922 3 22 3.552585101930454 3 23 3.3426823535622106 3 24 3.4524644403247535 3 25 3.6111186755477007 3 26 2.589642263631454 3 27 2.3596042519242317 3 28 3.0108524815099997 3 29 3.0027082302252666 3 30 2.496592741246616 3 31 2.3860507999301603 3 32 2.2976066215017283 3 33 1.9159334898945062 3 34 2.3842928504176086 3 35 2.5389387405975157 3 36 2.9314518993037666 3 37 3.0318368105281195 3 38 3.6625435899975756 3 39 4.35298219780195 3 40 8.223325604202259 3 41 7.990006561924037 3 42 3.5589392476314146 3 43 0.6564716804043051 3 44 0.8051130634762806 3 45 2.26527531783519 3 46 3.807222603552642 3 47 -1.3070425058532003 3 48 3.7420940229856967 3 49 5.868595306287826 3 50 3.0979340446336394 3 51 3.02034580678394 3 52 3.419799479580405 3 53 3.3874092035297245 3 54 4.943104748357499 3 55 3.60199659314479 3 56 5.118101118387692 4 1 1.0109608412330502 4 2 1.6369524198155005 4 3 2.9493619821802 4 4 1.355411257168491 4 5 1.8736328946631389 4 6 3.6966530581388426 4 7 1.1775458239940377 4 8 1.7669533782466047 4 9 1.93508611345532 4 10 1.3035701541277547 4 11 1.2411852086539952 4 12 4.2678886428916485 4 13 4.053319151671303 4 14 2.6563946679108454 4 15 3.477630213988782 4 16 3.7949838424836457 4 17 3.1953833827099203 4 18 4.985743824491671 4 19 3.2984150468388904 4 20 3.7295697242232038 4 21 2.7272073144462965 4 22 3.4525851019304525 4 23 2.117682353562209 4 24 4.4524644403247535 4 25 3.3111186755476965 4 26 2.1146422636314526 4 27 2.209604251924233 4 28 1.4108524815099983 4 29 2.6277082302252666 4 30 2.471592741246617 4 31 1.4860507999301618 4 32 1.5226066215017298 4 33 0.5159334898945005 4 34 1.8842928504176086 4 35 0.7139387405975199 4 36 3.5064518993037694 4 37 3.4818368105281152 4 38 3.887543589997577 4 39 4.527982197801954 4 40 8.398325604202256 4 41 10.390006561924036 4 42 6.683939247631415 4 43 7.956471680404302 4 44 6.105113063476278 4 45 5.965275317835189 4 46 6.782222603552643 4 47 6.417957494146801 4 48 6.042094022985694 4 49 9.293595306287823 4 50 6.072934044633641 4 51 3.945345806783937 4 52 3.5697994795804036 4 53 4.062409203529722 4 54 3.6181047483574993 4 55 4.10199659314479 4 56 4.09310111838769 5 1 0.835960841233053 5 2 1.7175079753710563 5 3 2.3521397599579785 5 4 1.360966812724051 5 5 1.4569662279964746 5 6 4.168875280361064 5 7 1.8275458239940363 5 8 1.6141756004688261 5 9 2.4073083356775413 5 10 1.7591257096833104 5 11 2.4106296530984395 5 12 4.215110865113871 5 13 4.41998581833797 5 14 4.07306133457751 5 15 4.574852436211003 5 16 5.317206064705864 5 17 4.173161160487702 5 18 5.524632713380555 5 19 3.526192824616672 5 20 2.032347502000981 5 21 2.957762870001851 5 22 3.3386962130415654 5 23 2.7899045757844334 5 24 1.544131106991415 5 25 2.4972297866588136 5 26 1.4507533747425683 5 27 2.8096042519242346 5 28 2.319185814843337 5 29 1.3943748968919323 5 30 1.5299260745799543 5 31 0.3916063554857203 5 32 1.5170510659461698 5 33 0.21593348989450334 5 34 0.8898484059731686 5 35 1.0278276294864028 5 36 2.5008963437482095 5 37 2.5818368105281166 5 38 3.1514324788864627 5 39 3.4557599755797312 5 40 7.042770048646702 5 41 9.456673228590699 5 42 5.342272580964753 5 43 7.309249458182084 5 44 7.482890841254058 5 45 5.8958308733907465 5 46 6.257222603552645 5 47 5.987401938591244 5 48 6.37820513409681 5 49 9.396373084065605 5 50 7.486822933522525 5 51 5.434234695672824 5 52 7.639243924024846 5 53 7.8846314257519445 5 54 7.368104748357499 5 55 7.121441037589232 5 56 8.073656673943248 6 1 1.1180121232843376 6 2 1.309388317251397 6 3 2.7923107001289154 6 4 1.4015651033223406 6 5 1.399273920304168 6 6 3.299217160702945 6 7 1.6544689009171165 6 8 1.795799532092758 6 9 1.274829703198911 6 10 0.9279291284867242 6 11 2.607210849679639 6 12 4.595452745455752 6 13 3.554601202953357 6 14 4.329471590987765 6 15 3.993655855014424 6 16 3.883445380945183 6 17 3.3633321006586385 6 18 4.007538696286538 6 19 3.3509791494029955 6 20 2.6199543396078155 6 21 2.991950904189885 6 22 2.59937997372532 6 23 1.7792208151006719 6 24 2.467208030068342 6 25 2.693810983240006 6 26 1.4870781610673518 6 27 1.1942196365396214 6 28 1.3512370968946144 6 29 2.2341184866355235 6 30 1.664541459195334 6 31 1.1565636204429808 6 32 1.9828630317581357 6 33 1.5172155411765544 6 34 -0.10160458547982643 6 35 1.8184259200846995 6 36 1.5885031813550512 6 37 1.2933752720665765 6 38 2.4420307694847594 6 39 2.9279821978019527 6 40 5.848325604202259 6 41 3.4630834850009578 6 42 5.021759760451928 6 43 6.877625526558152 6 44 6.801266909630126 6 45 4.712070189630058 6 46 5.814914911244951 6 47 5.350649801839108 6 48 4.504273510165184 6 49 6.84936453705706 6 50 4.578703275402873 6 51 5.833807345245479 6 52 5.265312300093221 6 53 4.52779381891434 6 54 4.9706688509216015 6 55 4.042381208529406 6 56 4.409126759413333 7 1 1.365372605938937 7 2 1.853128890403731 7 3 3.192009041003729 7 4 1.555411257168494 7 5 1.550103482898436 7 6 3.1084177640211976 7 7 2.4304870004646233 7 8 2.4654827900113077 7 9 2.920380231102378 7 10 2.6300407423630503 7 11 4.230891091006939 7 12 5.434065113479882 7 13 4.973907386965422 7 14 4.1711005502637875 7 15 5.323218449282898 7 16 5.462630901307172 7 17 4.99832455918051 7 18 5.547508530374024 7 19 5.366062105662422 7 20 3.7104520771643763 7 21 3.7845602556227647 7 22 4.962879219577509 7 23 3.170623530032799 7 24 4.259817381501222 7 25 3.7096480873124023 7 26 3.236701087160867 7 27 2.8390160166301186 7 28 2.441734834451175 7 29 2.771825877284094 7 30 2.2112986235995606 7 31 -0.34041978830513386 7 32 1.454959562678198 7 33 1.5835805487180323 7 34 1.0989987327705535 7 35 -0.12576714175542492 7 36 1.8946871934214116 7 37 0.3906603399398847 7 38 2.380190648821106 7 39 2.2515116095666556 7 40 6.377737368908143 7 41 -0.6658757910171431 7 42 4.773645129984359 7 43 5.296177562757247 7 44 5.013936592888047 7 45 2.428510611952838 7 46 4.154281427082058 7 47 4.807663376499743 7 48 3.8112116700445178 7 49 5.736242365111355 7 50 3.524404632868933 7 51 5.5894634538427646 7 52 5.327152420756875 7 53 5.58887979176502 7 54 6.426928277769267 7 55 5.985820122556557 7 56 4.2975128830935745 8 1 1.3637386190108316 8 2 1.6897301975932777 8 3 4.0743619821802 8 4 1.7498557016129368 8 5 1.3736328946631389 8 6 3.668875280361064 8 7 2.271990268438479 8 8 2.0863978226910476 8 9 3.18508611345532 8 10 2.7591257096833104 8 11 2.4661852086539966 8 12 4.853999754002764 8 13 5.142208040560192 8 14 5.100839112355288 8 15 4.102630213988782 8 16 3.8727616202614215 8 17 3.339827827154366 8 18 5.969077157825005 8 19 4.665081713505558 8 20 3.9212363908898666 8 21 2.763318425557408 8 22 3.9498073241526725 8 23 3.1787934646733262 8 24 2.7107977736580864 8 25 3.9972297866588136 8 26 2.8674200414092326 8 27 1.8651598074797917 8 28 1.2358524815100012 8 29 2.5610415635586037 8 30 2.057703852357733 8 31 1.3360507999301632 8 32 1.2948288437239484 8 33 1.549266823227839 8 34 0.2231817393064972 8 35 1.3611609628197385 8 36 1.5564518993037666 8 37 1.8318368105281166 8 38 1.6514324788864627 8 39 1.8724266422463955 8 40 4.098325604202259 8 41 -5.459993438075966 8 42 2.731161469853639 8 43 3.8648050137376373 8 44 3.4273352856985007 8 45 1.7291642067240787 8 46 2.090555936885977 8 47 2.3207352719245797 8 48 2.3226495785412524 8 49 4.896373084065605 8 50 2.0423784890780823 8 51 2.573123584561717 8 52 2.0281328129137357 8 53 2.162409203529723 8 54 4.284771415024167 8 55 2.621441037589232 8 56 3.0181011183876905 9 1 1.3081830634552745 9 2 2.161952419815499 9 3 2.9910286488468643 9 4 2.0276334793907154 9 5 1.7347440057742531 9 6 3.0855419470277283 9 7 2.021990268438479 9 8 1.808620044913269 9 9 2.93508611345532 9 10 2.036903487461089 9 11 3.3550740975428823 9 12 5.298444198447207 9 13 5.058874707226863 9 14 5.045283556799731 9 15 5.1859635473221175 9 16 4.4005393980392 9 17 4.589827827154366 9 18 5.830188268936112 9 19 4.887303935727779 9 20 4.004569724223202 9 21 3.013318425557408 9 22 3.672029546374894 9 23 3.9565712424510977 9 24 3.9607977736580864 9 25 3.6916742311032564 9 26 2.3674200414092326 9 27 2.226270918590899 9 28 3.2080747037322226 9 29 3.0888193413363823 9 30 2.613259407913283 9 31 2.3082730221523846 9 32 1.6559399548350626 9 33 1.299266823227839 9 34 1.7787372948620543 9 35 2.5000498517086314 9 36 3.278674121525988 9 37 3.2207256994170024 9 38 3.512543589997577 9 39 3.4557599755797312 9 40 6.6261033819800375 9 41 2.7344510063684773 9 42 4.592272580964753 9 43 5.503693902626527 9 44 5.8717797301429435 9 45 4.840275317835189 9 46 7.1461114924415305 9 47 7.765179716369023 9 48 7.350427356319031 9 49 9.313039750732269 9 50 7.459045155744754 9 51 7.76756802900616 9 52 6.944799479580404 9 53 7.46796475908528 9 54 7.784771415024164 9 55 6.621441037589232 9 56 8.101434451721026 10 1 1.585960841233053 10 2 1.9675079753710563 10 3 3.4910286488468643 10 4 1.860966812724051 10 5 1.6236328946631389 10 6 3.057764169249957 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13 -9.27928954398087 118 14 -5.071866201654373 118 15 -8.56041326427209 118 16 -10.915885722733748 118 17 -12.093747052072686 118 18 -9.60338661029094 118 19 -3.4472371270741498 118 20 -4.212821580124622 118 21 1.9674247057506449 118 22 2.336280754104365 118 23 0.9698562666056887 118 24 1.399203570759532 118 25 1.3426404146781366 118 26 2.8505118288488447 118 27 1.6356912084459765 118 28 2.8662872641186965 118 29 1.4124908389209168 118 30 2.4683318716814 118 31 1.3360507999301632 118 32 -6.775219465454796 118 33 2.16158566380755 118 34 0.4212493721567441 118 35 1.9867648275540404 118 36 1.3499301601733293 118 37 2.2666194192237654 118 38 1.7842827204323584 118 39 3.667112632584562 118 40 4.848325604202259 118 41 3.409571779315339 118 42 -2.9693216219338012 118 43 -12.055484841334826 118 44 -8.001408675654154 118 45 -3.5510290299908966 118 46 -11.797125222534312 118 47 -11.826607723244503 118 48 -11.287253803101262 118 49 -11.85966556327739 118 50 -9.217283346670708 118 51 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0.25760015656116764 119 34 0.7092928504176115 119 35 0.5972720739308528 119 36 1.973118565970431 119 37 2.623503477194781 119 38 2.0958769233309127 119 39 3.4279821978019527 119 40 7.139992270868923 119 41 -0.7933267714092977 119 42 -4.699394085701915 119 43 -13.926861652929029 119 44 -17.71155360319039 119 45 -6.243058015498143 119 46 -18.784444063114023 119 47 -18.66537583918653 119 48 -14.482905977014305 119 49 -17.089738027045506 119 50 -13.235399288699695 119 51 -11.746320859882728 119 52 -11.055200520419596 119 53 -10.879257463136945 119 54 -15.965228584975835 119 55 -9.864670073521879 119 56 -8.773565548278977 120 1 -0.5640391587669455 120 2 -0.12138091351783231 120 3 2.00769531551353 120 4 0.15541125716849535 120 5 -0.02636710533685971 120 6 2.3966530581388454 120 7 -0.15578750933929797 120 8 0.5752867115799347 120 9 0.48508611345531705 120 10 -1.5797631792055782 120 11 -5.700481458012671 120 12 -18.50711135710835 120 13 -17.896680848328693 120 14 -15.06027199875582 120 15 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-14.955200520419597 120 53 -14.354257463136944 120 54 -15.065228584975834 120 55 -14.506336740188544 120 56 -11.765232214945643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 151.0 5 22.0 6 18.0 7 33.0 8 29.0 9 28.0 10 29.0 11 37.0 12 34.0 13 40.0 14 47.0 15 76.0 16 83.0 17 124.0 18 154.0 19 169.0 20 214.0 21 257.0 22 352.0 23 449.0 24 445.0 25 522.0 26 577.0 27 675.0 28 781.0 29 908.0 30 1117.0 31 1284.0 32 1457.0 33 1658.0 34 1943.0 35 2371.0 36 3075.0 37 3694.0 38 3060.0 39 1430.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.36736634242668 0.4388210341549038 0.16455788780808894 0.02925473561032692 8 99.46244423316024 0.4132231404958678 0.04753894536678124 0.07679368097710816 9 98.33248007021137 1.14093468880275 0.3473999853726322 0.1791852556132524 10 50.5997220800117 34.04885540846925 8.571637533825788 6.7797849776932635 11 28.402691435676154 38.64550574124186 18.675491845242448 14.276310977839538 12 37.86294156366562 18.25861186279529 19.450742338916115 24.42770423462298 13 25.548646671543523 19.34162399414777 22.83467446964155 32.275054864667155 14 25.671969281404277 20.669226549643444 26.798317791186687 26.860486377765586 15 25.95769639649547 20.510282634993953 26.646871219619488 26.88514974889109 16 28.919057377049178 20.67476580796253 27.11504683840749 23.291129976580795 17 29.397352446427266 21.418123308710598 23.992540042419368 25.191984202442768 18 25.023769472683387 25.290718935127625 24.61054633218752 25.074965260001463 19 24.961575056722534 29.199297372465782 21.68996560052697 24.14916197028471 20 24.51497181345633 26.455084559631015 22.886009224687022 26.14393440222564 21 25.53572734586411 22.496891684341403 26.109851532216776 25.85752943757771 22 26.201272580999046 23.12586849996343 24.193666349740365 26.479192569297155 23 25.711255759526075 25.257807357566005 24.266803188766183 24.764133694141737 24 24.77510421999561 26.303664155635193 26.83756308052366 22.083668543845537 25 25.93161455476321 25.240446151033098 27.526055951727923 21.301883342475772 26 24.658035257113596 22.822032038621902 31.11330553726867 21.406627166995833 27 23.730922665885885 19.675731069062692 35.33652966365333 21.25681660139809 28 21.220440735046488 21.44007614027381 36.86214217731899 20.477340947360716 29 20.780598434413637 20.853756675689517 39.18355402736118 19.182090862535663 30 18.74908558888076 18.244330651060718 45.332845647403076 17.67373811265545 31 17.22207996488276 18.32680981819512 47.24000438965504 17.211105827267072 32 20.3205620814579 16.141544992132324 46.35708273868335 17.180810187726426 33 16.26995752160539 17.79698256921049 49.48732972022851 16.44573018895562 34 15.261058870879221 16.428231678314003 52.05810252094692 16.252606929859866 35 16.270305868578955 16.266647153519685 55.4844138738475 11.978633104053857 36 17.095924489646595 16.80324870125119 53.84502817004463 12.255798639057584 37 16.136713312330574 16.429775075097076 54.373946809290054 13.059564803282292 38 16.219485374972507 15.90792463895609 55.25254746719449 12.620042518876915 39 15.230769230769232 17.912087912087912 52.43223443223444 14.424908424908425 40 11.719468668359275 12.797586194208337 47.69842145932222 27.78452367811017 41 11.617874787994985 13.192242459995576 44.79389425558587 30.395988496423566 42 14.474021456555967 15.99721723847534 53.571820878034494 15.956940426934201 43 12.68777020590606 15.12053931266945 53.8726460027845 18.319044478639995 44 12.464183381088825 15.07236793769745 51.417970758944975 21.045477922268756 45 13.800051287687293 18.65772795545298 54.258709748323994 13.283511008535736 46 15.535367595882633 17.17279021209568 52.58068061101139 14.711161581010293 47 13.10264101251006 18.139585924354378 54.31633623527691 14.44143682785866 48 13.576765309112218 19.103428069147377 54.49018458833871 12.8296220334017 49 12.018965707354724 16.826551990296615 46.67181240122027 24.482669901128386 50 14.831222083913012 19.209928974152447 53.30599692465402 12.652852017280516 51 15.094408799266729 21.605866177818513 51.662694775435384 11.637030247479377 52 14.50485081457075 21.95130880468607 51.9311733479773 11.612667032765879 53 16.186813186813186 20.106227106227106 51.032967032967036 12.673992673992673 54 11.993404177354343 21.37779406375962 49.776474899230486 16.85232685965555 55 14.892993257109351 21.694517736734095 49.424655526238645 13.987833479917914 56 16.626982673357997 21.90190116854097 48.444997985274185 13.026118172826843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.0 9 4.0 10 4.5 11 5.0 12 5.0 13 25.0 14 45.0 15 74.0 16 103.0 17 116.0 18 129.0 19 205.5 20 282.0 21 349.0 22 459.5 23 503.0 24 574.5 25 646.0 26 794.5 27 943.0 28 1176.5 29 1569.5 30 1729.0 31 1860.0 32 1991.0 33 1936.5 34 1882.0 35 1696.5 36 1511.0 37 1243.5 38 816.5 39 657.0 40 546.5 41 436.0 42 402.0 43 368.0 44 367.5 45 367.0 46 407.0 47 555.0 48 663.0 49 734.5 50 806.0 51 797.0 52 788.0 53 783.0 54 817.5 55 857.0 56 849.0 57 841.0 58 864.0 59 887.0 60 979.0 61 1071.0 62 1085.5 63 1088.0 64 1076.0 65 1001.0 66 926.0 67 849.5 68 773.0 69 686.5 70 600.0 71 518.0 72 414.0 73 392.0 74 298.0 75 204.0 76 175.0 77 146.0 78 112.5 79 56.0 80 33.0 81 26.5 82 20.0 83 15.5 84 11.0 85 7.0 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.021941051707745192 14 0.003656841951290865 15 0.24500841073648796 16 0.06582315512323558 17 0.0 18 0.0 19 0.0731368390258173 20 0.10239157463614423 21 0.0 22 0.0 23 0.0 24 0.0 25 0.003656841951290865 26 0.01462736780516346 27 0.08410736487968991 28 0.10239157463614423 29 0.02925473561032692 30 0.021941051707745192 31 0.03291157756161779 32 0.06947999707452644 33 0.13895999414905288 34 0.054852629269362976 35 0.05119578731807212 36 0.043882103415490384 37 0.17552841366196154 38 0.23403788488261537 39 0.16821472975937982 40 0.6180062897681562 41 0.8191325970891539 42 0.1279894682951803 43 0.19015578146712497 44 0.45344840196006725 45 0.1791852556132524 46 0.17187157171067066 47 0.02925473561032692 48 0.15358736195421632 49 0.5083010312294303 50 0.11701894244130769 51 0.25963577854165143 52 0.11336210049001681 53 0.16821472975937982 54 0.20478314927228847 55 0.2120968331748702 56 0.17187157171067066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 27346.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.15007679368098 #Duplication Level Percentage of deduplicated Percentage of total 1 86.57318478678448 65.92554669787172 2 7.769880906646177 11.833540554377239 3 2.612370341913177 5.967966064506692 4 1.2341529004994238 3.7592335259270095 5 0.5522474068382636 2.1026841219922474 6 0.2929312331924702 1.3384041541724567 7 0.2353054168267384 1.2542967892927668 8 0.1536688436419516 0.9361515395304615 9 0.1536688436419516 1.0531704819717693 >10 0.41298501728774495 5.218313464492065 >50 0.004802151363810988 0.2011263073209976 >100 0.004802151363810988 0.40956629854457693 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 112 0.40956629854457693 No Hit GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 55 0.2011263073209976 No Hit GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.16090104585679807 No Hit GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 37 0.135303152197762 No Hit GCAGGAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA 36 0.13164631024647117 No Hit GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 36 0.13164631024647117 No Hit GCAGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.1279894682951803 No Hit GCAGGAGGGAATGTTAATTTAACTAACTTGTCTTTTTTAGCCTGTGCTTAATTTAA 34 0.1243326263438894 No Hit GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 33 0.12067578439259855 No Hit GCAGGAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 33 0.12067578439259855 No Hit GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 32 0.11701894244130769 No Hit GCAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 29 0.1060484165874351 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.003656841951290865 0.0 0.0 0.0 0.0 7 0.003656841951290865 0.0 0.0 0.0 0.0 8 0.003656841951290865 0.0 0.0 0.0 0.0 9 0.00731368390258173 0.0 0.0 0.0 0.0 10 0.00731368390258173 0.0 0.0 0.0 0.0 11 0.010970525853872596 0.0 0.0 0.0 0.0 12 0.018284209756454325 0.0 0.0 0.0 0.0 13 0.025597893659036055 0.0 0.0 0.0 0.0 14 0.025597893659036055 0.0 0.0 0.0 0.0 15 0.025597893659036055 0.0 0.0 0.0 0.0 16 0.025597893659036055 0.0 0.0 0.0 0.0 17 0.02925473561032692 0.0 0.0 0.0 0.0 18 0.02925473561032692 0.0 0.0 0.0 0.0 19 0.02925473561032692 0.0 0.0 0.0 0.0 20 0.02925473561032692 0.0 0.0 0.0 0.0 21 0.032911577561617784 0.0 0.0 0.0 0.0 22 0.03656841951290865 0.0 0.0 0.0 0.0 23 0.04022526146419952 0.0 0.0 0.0 0.0 24 0.04022526146419952 0.0 0.0 0.0 0.0 25 0.05119578731807211 0.0 0.0 0.0 0.0 26 0.06216631317194471 0.0 0.0 0.0 0.0 27 0.06216631317194471 0.0 0.0 0.0 0.0 28 0.06947999707452644 0.0 0.0 0.0 0.0 29 0.08045052292839903 0.0 0.0 0.0 0.0 30 0.0841073648796899 0.0 0.0 0.0 0.0 31 0.09873473268485336 0.0 0.0 0.0 0.0 32 0.09873473268485336 0.0 0.0 0.0 0.0 33 0.09873473268485336 0.0 0.0 0.0 0.0 34 0.09873473268485336 0.0 0.0 0.0 0.0 35 0.09873473268485336 0.0 0.0 0.0 0.0 36 0.10239157463614422 0.0 0.0 0.0 0.0 37 0.10239157463614422 0.0 0.0 0.0 0.0 38 0.10239157463614422 0.0 0.0 0.0 0.0 39 0.10239157463614422 0.0 0.0 0.0 0.0 40 0.10239157463614422 0.0 0.0 0.0 0.0 41 0.1060484165874351 0.0 0.0 0.0 0.0 42 0.11336210049001683 0.0 0.0 0.0 0.0 43 0.11701894244130769 0.0 0.0 0.0 0.0 44 0.12067578439259855 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCACA 20 3.8591743E-4 50.64366 39 ACGCCCA 15 0.007930726 50.643658 39 CGCCCAG 15 0.007930726 50.643658 40 TACGCCC 15 0.007930726 50.643658 38 TACCCAC 15 0.007930726 50.643658 38 ACACCGC 15 0.007989538 50.549347 37 AGCCCTG 15 0.007989538 50.549347 37 CCTGCAC 15 0.008048674 50.45539 36 GCGGCCA 15 0.008228038 50.175602 44 TCTCAAC 20 4.0407866E-4 50.175602 43 CCCAGTT 15 0.008288482 50.083027 42 CAGCAAA 20 4.0779103E-4 50.083027 46 AACGATT 20 4.0779103E-4 50.083027 47 GTTCTGA 35 4.971662E-8 50.083027 46 GCAGCAA 15 0.008288482 50.083027 45 GGCCAAG 20 4.0779103E-4 50.083027 46 ACGATTA 20 4.0779103E-4 50.083027 48 CGGCCAA 20 4.0779103E-4 50.083027 45 CAAGGAG 15 0.008288482 50.083027 49 AATGTTT 15 0.008288482 50.083027 49 >>END_MODULE