FastQCFastQC Report
Fri 17 Jun 2016
SRR1526257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29160
Sequences flagged as poor quality0
Sequence length56
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.24348422496570646No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.23319615912208505No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG660.22633744855967078No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC450.15432098765432098No Hit
AGATGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG370.12688614540466392No Hit
AGATGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG340.11659807956104253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAACC309.704072E-750.30849535
TGATATG150.00820404850.22145550
CATTTGA150.00826043750.13471646
TTGATAT150.00826043750.13471649
AGCATTT150.00831711550.0482844
AATGGCT204.096714E-450.04827529
GTTGATC150.00837408249.9621416
TTCGTGG252.0463262E-549.96213515
ATTCGTG252.0463262E-549.96213514
ACAGCCA150.00843133849.8762912
AATTCGT204.1671286E-449.8762913
TGGTAAA150.00843133849.8762913
TGATCCT150.00843133849.8762918
CAACAGC150.00843133849.8762910
TGGAGAA204.1671286E-449.8762919
CATTATC150.00843133849.8762910
AGAAATG204.1671286E-449.8762926
GAATTCG204.1671286E-449.8762912
GGGAATT301.0302174E-649.8762910
TACCTGG150.00843133849.8762910
Produced by FastQC (version 0.11.5)