##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526257_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29160 Sequences flagged as poor quality 0 Sequence length 56 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.87887517146776 39.0 38.0 40.0 35.0 40.0 2 37.256721536351165 39.0 38.0 40.0 33.0 40.0 3 37.38134430727023 39.0 38.0 39.0 33.0 40.0 4 37.23943758573388 39.0 38.0 40.0 33.0 40.0 5 37.10229766803841 39.0 38.0 40.0 33.0 40.0 6 37.106550068587104 39.0 38.0 40.0 33.0 40.0 7 37.425 39.0 38.0 40.0 34.0 40.0 8 37.32403978052126 39.0 38.0 40.0 33.0 40.0 9 37.21796982167353 39.0 38.0 40.0 33.0 40.0 10 36.83360768175583 39.0 36.0 40.0 32.0 40.0 11 35.67705761316873 38.0 35.0 39.0 29.0 40.0 12 34.05401234567901 38.0 33.0 39.0 21.0 40.0 13 33.94266117969822 38.0 33.0 39.0 20.0 40.0 14 34.14269547325103 38.0 33.0 39.0 22.0 40.0 15 34.08737997256516 38.0 33.0 39.0 21.0 40.0 16 33.38412208504801 38.0 31.0 39.0 19.0 40.0 17 33.391975308641975 38.0 31.0 39.0 20.0 40.0 18 32.8656378600823 37.0 30.0 39.0 18.0 40.0 19 33.76855281207133 37.0 31.0 39.0 23.0 40.0 20 33.75017146776406 38.0 31.0 39.0 23.0 40.0 21 34.5610768175583 38.0 33.0 39.0 24.0 40.0 22 34.322599451303155 38.0 33.0 39.0 23.0 40.0 23 34.74084362139918 38.0 33.0 39.0 25.0 40.0 24 34.40294924554184 38.0 33.0 39.0 24.0 40.0 25 34.48906035665295 38.0 33.0 39.0 24.0 40.0 26 35.277023319615914 38.0 35.0 39.0 27.0 40.0 27 35.183573388203015 38.0 35.0 39.0 27.0 40.0 28 35.127949245541835 38.0 35.0 39.0 27.0 40.0 29 35.01536351165981 38.0 35.0 39.0 27.0 40.0 30 34.98336762688614 38.0 35.0 39.0 26.0 40.0 31 35.37688614540466 38.0 35.0 39.0 28.0 40.0 32 35.07417695473251 38.0 35.0 39.0 27.0 40.0 33 35.25246913580247 38.0 35.0 39.0 28.0 40.0 34 35.20123456790123 38.0 35.0 39.0 28.0 40.0 35 35.08624828532236 38.0 35.0 39.0 28.0 40.0 36 34.1650548696845 38.0 33.0 39.0 26.0 40.0 37 33.94238683127572 37.0 33.0 39.0 26.0 40.0 38 33.65058299039781 37.0 32.0 39.0 24.0 40.0 39 33.39334705075446 37.0 32.0 39.0 23.0 40.0 40 29.839746227709192 35.0 24.0 39.0 8.0 40.0 41 26.272770919067217 31.0 14.0 38.0 5.0 39.0 42 31.028840877914952 35.0 30.0 38.0 10.0 40.0 43 29.273731138545955 34.0 26.0 38.0 4.0 39.0 44 29.202229080932785 34.0 26.0 38.0 4.0 39.0 45 30.545061728395062 35.0 29.0 38.0 4.0 39.0 46 30.238923182441702 35.0 28.0 38.0 4.0 40.0 47 29.66093964334705 35.0 27.0 38.0 4.0 40.0 48 29.830692729766803 35.0 27.0 38.0 4.0 40.0 49 26.993758573388202 33.0 14.0 38.0 4.0 40.0 50 29.478943758573386 35.0 27.0 38.0 4.0 40.0 51 29.766598079561042 35.0 28.0 39.0 4.0 40.0 52 29.537311385459535 35.0 27.0 39.0 4.0 40.0 53 29.049897119341562 35.0 26.0 39.0 4.0 40.0 54 28.03460219478738 35.0 18.0 39.0 4.0 40.0 55 28.435528120713307 35.0 23.0 39.0 4.0 40.0 56 27.279046639231826 34.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.8757678187971223 1 2 1.6030909956398318 1 3 1.0717951325025794 1 4 1.9936435799981567 1 5 1.6559612974094549 1 6 1.9965502479295765 1 7 1.6191931794980619 1 8 1.828888147070458 1 9 1.7701201271705855 1 10 1.4860363550474318 1 11 -3.1824431102330415 1 12 -15.933584265580567 1 13 -6.658127545300225 1 14 -3.9725273492614868 1 15 -4.630270954741135 1 16 -22.50753310554134 1 17 -4.648473396350109 1 18 -13.89704895431684 1 19 -4.785708085033594 1 20 -5.555798516268823 1 21 -6.872048249746381 1 22 -3.071890110481064 1 23 -3.6150263434500687 1 24 -5.8247312708996475 1 25 -3.2538381076491483 1 26 2.935941748717923 1 27 2.848084368711149 1 28 3.1228650243547875 1 29 3.2365053074436787 1 30 3.2447918915348666 1 31 2.2933348630564723 1 32 2.8023959445909057 1 33 2.3597697627979457 1 34 1.3375017767663522 1 35 2.374520726638046 1 36 2.6691123512207184 1 37 2.9163869630339576 1 38 1.7335029375110622 1 39 2.90991942688877 1 40 6.172628026002009 1 41 -7.953749905219727 1 42 -9.614382964260383 1 43 -15.35884509675884 1 44 -21.844845952024258 1 45 -9.75559359664743 1 46 -14.394952314686464 1 47 -22.447028169123076 1 48 -13.034751033775997 1 49 -19.049391283666857 1 50 -12.207044980742399 1 51 -9.368322785118245 1 52 -8.858023971297012 1 53 -8.598060452836794 1 54 -7.729702639868886 1 55 -7.614937415286541 1 56 -2.785351230207773 2 1 1.0500102430395515 2 2 2.2318788744277143 2 3 1.086946647654095 2 4 2.7133405496951255 2 5 2.3655572570054133 2 6 2.4334189347982615 2 7 2.3262638865687677 2 8 1.6622214804037938 2 9 2.510019117069575 2 10 1.7865414055524838 2 11 1.860486182696249 2 12 -0.9032812352775359 2 13 0.5514684142957336 2 14 3.8532302264960876 2 15 3.4025573280871484 2 16 3.7475173995091637 2 17 4.005567007690292 2 18 0.3580015507336647 2 19 3.843079793754285 2 20 3.239656029185724 2 21 3.766840639142508 2 22 3.814473525882569 2 23 4.2713372929135645 2 24 1.9884000422316674 2 25 3.4077780539670144 2 26 3.8273558901320683 2 27 2.7293975000242767 2 28 3.8097337112234726 2 29 3.789535610473976 2 30 3.222064618807593 2 31 2.500405570127178 2 32 3.211486853681812 2 33 2.713305116333295 2 34 3.3930573323219093 2 35 2.9174500195673403 2 36 4.297900230008601 2 37 3.944164740811736 2 38 4.652694856702986 2 39 4.647293164262507 2 40 9.112021965395947 2 41 2.253320801850979 2 42 1.0245059246285066 2 43 -4.348744086657831 2 44 -9.51908837626668 2 45 0.9893558983020654 2 46 -5.167679587413737 2 47 -15.224805946900855 2 48 -4.221619720644686 2 49 -9.47363370790928 2 50 -2.916640940338361 2 51 1.5256166088211494 2 52 0.7909659276928842 2 53 1.300929446153102 2 54 1.4622165520503074 2 55 1.1072848069356809 2 56 5.138891194034649 3 1 1.3835197567816238 3 2 1.9422382824615383 3 3 1.3121052100219615 3 4 2.5711629598431216 3 5 2.584901865884909 3 6 2.2103745373352623 3 7 1.7638960218753184 3 8 2.2203610152875157 3 9 2.1796808506847967 3 10 1.7580001793368396 3 11 2.70773776831993 3 12 3.95401263364424 3 13 3.724301395268249 3 14 2.7987904801958763 3 15 3.4078427403493023 3 16 4.6481516489806225 3 17 4.626074407267463 3 18 4.630728823460938 3 19 3.9604159459741552 3 20 4.322108460475363 3 21 2.9761429647239055 3 22 2.759505238356148 3 23 3.611717842596441 3 24 3.2928397885318788 3 25 3.5129577579839264 3 26 1.643952084635238 3 27 2.1458879862822045 3 28 1.7611079184116392 3 29 1.1299161601568528 3 30 1.1575825892092837 3 31 0.9903632868290799 3 32 2.0582098980792765 3 33 1.461190951428435 3 34 2.5500340765079557 3 35 0.8984225354235775 3 36 2.2117480101354516 3 37 2.0778856710442923 3 38 1.7483608186480168 3 39 3.0119866103513004 3 40 5.641620274064024 3 41 8.493278518552884 3 42 4.59427336648897 3 43 1.562989528564156 3 44 2.3741462960377575 3 45 4.501512346505024 3 46 2.802722103918189 3 47 -2.591085016668295 3 48 2.9258432814694793 3 49 5.157973057418417 3 50 3.4802935205495906 3 51 1.0309020210833069 3 52 0.5747925661706859 3 53 1.3173142241657878 3 54 2.1107366366169025 3 55 1.2642615511217272 3 56 2.518912335683698 4 1 1.4532871986420872 4 2 2.453866189438287 4 3 2.242337768161498 4 4 2.803721099378002 4 5 2.9569948891407236 4 6 2.6522350024515404 4 7 2.252268114898577 4 8 2.383151712961933 4 9 2.528518059987121 4 10 2.548697853755442 4 11 4.103086605529235 4 12 4.837733563876796 4 13 5.07313860457058 4 14 4.705767224381923 4 15 5.152028786860932 4 16 5.9272214164224835 4 17 6.044679058430251 4 18 6.537705567646984 4 19 5.5650671087648576 4 20 5.950015437219548 4 21 4.208701104258786 4 22 5.015319191844519 4 23 4.448927144922024 4 24 4.176560718764435 4 25 4.8152833393792775 4 26 3.4346497590538405 4 27 3.634260079305463 4 28 4.133200941667447 4 29 4.478753369459177 4 30 3.8552570078139397 4 31 3.292688868224431 4 32 4.104721525986257 4 33 1.7170049049168057 4 34 2.015150355577724 4 35 3.0379574191445116 4 36 4.65360847525173 4 37 4.984862415230339 4 38 5.632081748880573 4 39 5.75617265686293 4 40 8.827666785691932 4 41 10.725836658087765 4 42 6.780319878116877 4 43 8.307175575075789 4 44 6.816006761154039 4 45 7.687558858132931 4 46 6.570163964383305 4 47 4.757752192634026 4 48 5.763052583795059 4 49 10.018438173697486 4 50 6.3175028228751735 4 51 7.1936927187577275 4 52 7.621304194077663 4 53 7.038244456723927 4 54 8.180504078477366 4 55 7.427052248796144 4 56 6.914261172893003 5 1 1.4710059140352172 5 2 2.0911862337350726 5 3 1.8813189420263896 5 4 2.6841197704743465 5 5 1.6480247894729487 5 6 1.7862327876121142 5 7 1.8890344493393272 5 8 2.4003167184990346 5 9 1.9288502859007437 5 10 2.2757188947299696 5 11 3.9524775246875947 5 12 5.233082401086101 5 13 4.643459756287079 5 14 5.098901222167086 5 15 3.6990941246239473 5 16 5.020244672236437 5 17 5.228510730634017 5 18 5.725966918699033 5 19 5.535720486394979 5 20 4.670391959921652 5 21 3.576364448666318 5 22 4.0590622704713155 5 23 3.0397355613118364 5 24 3.207014760846384 5 25 4.480288876477836 5 26 2.5708623836385556 5 27 2.70125897188575 5 28 2.7815951830849457 5 29 2.796029116967489 5 30 3.7566966534396258 5 31 2.4560332757548835 5 32 2.27858642078138 5 33 2.8637380167661988 5 34 2.4525811418457195 5 35 2.4499175520348757 5 36 4.6214933036016745 5 37 4.098926645573641 5 38 4.705725159733284 5 39 4.536903553872897 5 40 9.767866121240104 5 41 12.173234221764403 5 42 7.976886877009456 5 43 9.018139030225285 5 44 8.905154047975746 5 45 7.609485768431938 5 46 6.271714351980201 5 47 7.453765481670576 5 48 5.96128071225575 5 49 10.24822776395219 5 50 6.9199391462417275 5 51 4.345962929167474 5 52 4.84832523505219 5 53 4.905907801131459 5 54 6.004424344258101 5 55 5.857284806935681 5 56 7.297982103125559 6 1 2.0261743228621683 6 2 2.613253597265853 6 3 1.8929333438846925 6 4 3.046489108453443 6 5 2.836178099577481 6 6 3.2682304647317437 6 7 2.6056430439967 6 8 2.7841726999159917 6 9 2.4218816447857634 6 10 2.4528385230691114 6 11 4.062814342341483 6 12 5.159911669378786 6 13 5.48550389101414 6 14 5.6819441954539585 6 15 4.931382161790033 6 16 6.020244672236437 6 17 5.470090289286748 6 18 6.630728823460938 6 19 6.090308175129003 6 20 5.596640508121425 6 21 4.254645517191285 6 22 5.080548913909183 6 23 5.073998046793832 6 24 5.068222658639648 6 25 4.714319073922667 6 26 3.762500014300578 6 27 3.088599273860197 6 28 3.5103988996935414 6 29 4.006830510695707 6 30 3.775279696412916 6 31 3.5053944836526725 6 32 3.9487374080055417 6 33 3.8468971340716536 6 34 3.423545137199959 6 35 3.638070862139408 6 36 4.709762757724789 6 37 3.8039208383727114 6 38 4.75857068818857 6 39 5.331328640980914 6 40 8.875880058522334 6 41 4.861968251961844 6 42 6.622066900238259 6 43 8.15402753196745 6 44 6.925479251227774 6 45 5.356872528013817 6 46 7.470901343850123 6 47 7.622755028708898 6 48 7.144788261616959 6 49 10.806299773243712 6 50 7.844445534162748 6 51 9.16419754008501 6 52 8.64739608290353 6 53 7.864676674534476 6 54 5.795365110808621 6 55 5.991431148399094 6 56 5.700421127515803 7 1 1.3835197567816238 7 2 2.1282847940894456 7 3 1.0562912565335907 7 4 2.5479071458896314 7 5 2.189553028675604 7 6 2.7685140722189843 7 7 2.2057564869915964 7 8 2.0808261315665817 7 9 1.7378203855685186 7 10 2.0370699467787006 7 11 2.68448195436644 7 12 5.163314959225637 7 13 5.0963944185240635 7 14 4.147627689498208 7 15 4.9892380891865145 7 16 5.276058625724808 7 17 4.672586035174437 7 18 5.328403242065587 7 19 4.35576478318346 7 20 5.438387530242807 7 21 4.208701104258786 7 22 4.085086633704982 7 23 3.2628806332941167 7 24 3.3858630443458324 7 25 3.559469385890907 7 26 2.9927892939375624 7 27 3.238911242096158 7 28 1.295991639341871 7 29 3.153171974110343 7 30 2.8087453799069593 7 31 1.2694330542709409 7 32 2.593093619009508 7 33 2.5542142072423886 7 34 2.8058480299963264 7 35 1.8751667214700873 7 36 3.5838410333912663 7 37 3.2406763687187095 7 38 3.8413840744619705 7 39 4.314312191746652 7 40 7.525341204296581 7 41 1.5630459604133478 7 42 5.989622203698275 7 43 7.911826737866484 7 44 7.816006761154039 7 45 5.38523327673758 7 46 4.15155931322051 7 47 4.618217308913099 7 48 4.0188665372834365 7 49 7.971926545790506 7 50 3.1779679391542395 7 51 4.0541578350367935 7 52 3.365490240589292 7 53 3.5266165497471853 7 54 4.157248264523879 7 55 2.566587132517075 7 56 2.9375169868464894 8 1 1.6591011521304608 8 2 2.3114243289731675 8 3 2.3301284658359123 8 4 3.176976913331494 8 5 2.9337390751872334 8 6 3.0493280257073536 8 7 2.6830820683869447 8 8 2.787221480403794 8 9 2.9145645716150312 8 10 2.713814132825213 8 11 3.453668000878068 8 12 5.658082401086098 8 13 4.6946502324775565 8 14 5.694139317405181 8 15 5.70028460081442 8 16 6.520244672236437 8 17 6.673748825872114 8 18 6.780728823460937 8 19 5.722625248299735 8 20 5.194201483731177 8 21 4.641840639142508 8 22 4.403109889518937 8 23 4.28497365654993 8 24 4.322490951322571 8 25 3.960050781239744 8 26 3.2387195264957 8 27 3.2953065909333716 8 28 3.2506428021325675 8 29 3.1281719741103444 8 30 3.0697918915348694 8 31 2.732223751945355 8 32 2.9523959445909043 8 33 2.0542142072423886 8 34 2.5680573323219065 8 35 3.510631837749159 8 36 3.502445684554054 8 37 3.394164740811739 8 38 3.239058493066622 8 39 4.190474982444329 8 40 8.039294692668676 8 41 -7.089861016330836 8 42 3.199505924628504 8 43 6.921710458796717 8 44 6.2218207146424085 8 45 5.591628625574792 8 46 6.055047685313539 8 47 6.875194053099147 8 48 6.687471188446224 8 49 9.067275382999814 8 50 7.115177241479824 8 51 7.398343881548424 8 52 7.686420473147432 8 53 7.69638399160765 8 54 8.82585291568667 8 55 8.10728480693568 8 56 7.1979821031255575 9 1 0.9341011521304594 9 2 1.3114243289731675 9 3 1.4551284658359123 9 4 1.3269769133314924 9 5 2.0587390751872334 9 6 1.3493280257073579 9 7 0.858082068386949 9 8 1.337221480403791 9 9 0.9145645716150312 9 10 0.9388141328252075 9 11 2.3536680008780664 9 12 2.483082401086101 9 13 4.469650232477555 9 14 4.96913931740518 9 15 4.275284600814423 9 16 4.22024467223644 9 17 5.173748825872114 9 18 5.555728823460935 9 19 3.622625248299741 9 20 4.169201483731179 9 21 3.2918406391425066 9 22 2.728109889518933 9 23 3.3849736565499313 9 24 3.1724909513225725 9 25 3.1600507812397396 9 26 2.4137195264957043 9 27 3.2453065909333674 9 28 2.400642802132566 9 29 2.528171974110343 9 30 2.4447918915348694 9 31 1.057223751945358 9 32 0.22739594459090284 9 33 1.0042142072423914 9 34 0.46805733232191216 9 35 1.3356318377491618 9 36 0.7524456845540541 9 37 2.2191647408117348 9 38 2.564058493066618 9 39 2.840474982444327 9 40 5.214294692668673 9 41 3.335138983669161 9 42 2.8495059246285095 9 43 5.3967104587967185 9 44 3.9718207146424085 9 45 3.4416286255747934 9 46 4.1050476853135365 9 47 4.1501940530991455 9 48 2.9374711884462243 9 49 6.017275382999809 9 50 2.940177241479823 9 51 3.0983438815484234 9 52 2.4864204731474295 9 53 3.0213839916076495 9 54 3.8258529156866707 9 55 2.4072848069356816 9 56 2.9729821031255597 10 1 1.8233868664161719 10 2 2.143567186116023 10 3 2.0622713229787735 10 4 2.8555483419029244 10 5 2.6194533609015167 10 6 2.6243280257073565 10 7 1.965224925529803 10 8 1.8050786232609397 10 9 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115 46 -1.6190902457209475 115 47 -1.4748059469008545 115 48 -1.1142529494848112 115 49 -12.356862548034671 115 50 -0.7779262067960389 115 51 -1.408552670175716 115 52 -1.5342691820249819 115 53 -2.231202215288903 115 54 -6.424147084313329 115 55 -0.9530600206505255 115 56 1.2462579651945234 116 1 1.238512916836342 116 2 1.8040713877966965 116 3 1.4345402305417991 116 4 2.0916827956844344 116 5 2.1690331928342914 116 6 1.200798613942652 116 7 1.7727879507398896 116 8 2.13280971569791 116 9 1.7145645716150284 116 10 1.4844023681193264 116 11 -1.6595672932395757 116 12 -10.17868230479625 116 13 -5.645055649875388 116 14 -1.305860682594819 116 15 -7.765891869773814 116 16 -2.3032847395282694 116 17 -0.8527217623631813 116 18 -5.486918235362591 116 19 -0.6435512222884974 116 20 -2.51168086921 116 21 4.325664168554276 116 22 4.119286360107168 116 23 3.9585030683146343 116 24 4.573961539557871 116 25 4.414462545945625 116 26 3.4784254088486435 116 27 2.9967771791686673 116 28 2.988878096250211 116 29 3.653171974110343 116 30 3.7594977738878086 116 31 2.138106104886532 116 32 3.8612194740026737 116 33 2.2600965601835625 116 34 2.5548220382042643 116 35 2.4709259553962184 116 36 4.090680978671699 116 37 4.576517681988207 116 38 5.009646728360735 116 39 4.61253380597374 116 40 9.398118222080441 116 41 6.964550748375046 116 42 0.4362706305108581 116 43 -10.082701305909168 116 44 -10.016414579475239 116 45 1.437216860868908 116 46 -1.836128785274699 116 47 0.731076406040323 116 48 -2.238999399789069 116 49 -9.485665793470776 116 50 -1.5436462879319421 116 51 0.32775564625430675 116 52 -0.0018148209702175677 116 53 -0.43302777309823526 116 54 -8.218264731372152 116 55 0.32787304222979685 116 56 2.6215115148902655 117 1 0.18910115213045486 117 2 0.806424328973165 117 3 0.6451284658359171 117 4 0.986976913331489 117 5 1.4937390751872357 117 6 1.3843280257073545 117 7 0.5080820683869476 117 8 0.18222148040379693 117 9 -0.32543542838497075 117 10 0.9338141328252121 117 11 -3.3313319991219323 117 12 -6.8469175989138975 117 13 -6.560349767522446 117 14 -12.205860682594817 117 15 -7.304715399185579 117 16 -16.219755327763565 117 17 -8.916251174127886 117 18 -9.889271176539062 117 19 -3.642374751700263 117 20 -4.185798516268822 117 21 2.6868406391425097 117 22 3.218109889518935 117 23 2.6349736565499313 117 24 2.2174909513225742 117 25 2.2050507812397413 117 26 2.153719526495699 117 27 2.50030659093337 117 28 1.9806428021325644 117 29 1.7531719741103444 117 30 2.2347918915348686 117 31 1.2322237519453552 117 32 1.642395944590909 117 33 1.7142142072423852 117 34 1.1630573323219124 117 35 1.4956318377491584 117 36 3.5224456845540573 117 37 1.874164740811736 117 38 1.419058493066622 117 39 2.3254749824443266 117 40 7.099294692668671 117 41 4.775138983669162 117 42 -1.155494075371493 117 43 -7.793289541203283 117 44 -12.56817928535759 117 45 -3.2333713744252073 117 46 -5.174952314686465 117 47 -5.074805946900856 117 48 -4.882528811553776 117 49 -7.672724617000188 117 50 -5.904822758520179 117 51 -6.801656118451579 117 52 -6.593579526852569 117 53 -7.763616008392351 117 54 -10.66414708431333 117 55 -5.202715193064318 117 56 -4.162017896874442 118 1 0.3519582949876039 118 2 1.4007100432588828 118 3 0.797985608693061 118 4 1.362691199045777 118 5 1.612310503758664 118 6 1.667185168564501 118 7 1.1152249255298017 118 8 1.1979357661180785 118 9 1.2859931430435978 118 10 1.2280998471109257 118 11 -0.8927605705505002 118 12 -4.445489027485326 118 13 -8.45177833895102 118 14 -10.591574968309104 118 15 -4.146143970614151 118 16 -11.979755327763563 118 17 -8.604822602699315 118 18 -9.904985462253347 118 19 -3.130946180271689 118 20 -3.1629413734116802 118 21 1.2311263534282233 118 22 2.142395603804651 118 23 1.813545085121362 118 24 1.2189195227511433 118 25 1.5636222098111716 118 26 2.785148097924271 118 27 2.998878019504801 118 28 3.186357087846851 118 29 2.403171974110343 118 30 2.459077605820582 118 31 0.4679380376596427 118 32 -6.519032626837667 118 33 0.19707135009953447 118 34 0.6430573323219093 118 35 1.021346123463445 118 36 2.6095885416969082 118 37 2.5513075979545903 118 38 2.0390584930666193 118 39 1.8226178395871813 118 40 6.696437549811527 118 41 3.79228184081202 118 42 -2.832636932514351 118 43 -11.303289541203284 118 44 -10.249607856786163 118 45 -4.997657088710923 118 46 -11.57352374325789 118 47 -13.546234518329426 118 48 -12.633957382982347 118 49 -15.847010331285903 118 50 -11.62767990137732 118 51 -10.451656118451577 118 52 -10.770722383995427 118 53 -9.117901722678067 118 54 -16.031289941456187 118 55 -9.285572335921461 118 56 -6.523446468303014 119 1 -0.2524373094080019 119 2 -0.8135756710268325 119 3 -0.2102561495486981 119 4 0.7885153748699523 119 5 0.7029698444179999 119 6 0.48586648724581494 119 7 0.5465436068484877 119 8 0.6622214804037938 119 9 0.2914876485381086 119 10 0.013814132825210379 119 11 -6.725178152968084 119 12 -13.613071445067746 119 13 -15.76496515213783 119 14 -9.882783759517896 119 15 -7.593946168416348 119 16 -11.018216866225103 119 17 -13.214712712589428 119 18 -14.138501945769832 119 19 -8.125451674777185 119 20 -8.075029285499593 119 21 -2.6177747454728753 119 22 -0.283428572019524 119 23 -1.7111801896039154 119 24 -2.9486628948312728 119 25 -1.345718449529489 119 26 -3.0170497042735285 119 27 -0.9873857167589364 119 28 -1.599357197867434 119 29 -3.616058795120427 119 30 -2.4282850315420568 119 31 -4.029314709593102 119 32 -7.312988670793711 119 33 -3.253478100449918 119 34 -2.6646349753703973 119 35 -2.6105220084046863 119 36 -1.6129389308305626 119 37 -0.6904506438036506 119 38 -1.845556891548764 119 39 -1.3037557867864429 119 40 2.9931408465148266 119 41 -0.8340917855616077 119 42 -6.360109459986877 119 43 -15.745597233510976 119 44 -15.274333131511437 119 45 -8.917986759040591 119 46 -16.125721545455693 119 47 -15.513267485362393 119 48 -13.024067273092237 119 49 -15.671186155461728 119 50 -12.330976604674024 119 51 -10.201656118451577 119 52 -9.298194911467952 119 53 -11.519000623776966 119 54 -15.962608622774868 119 55 -12.14271519306432 119 56 -9.702017896874441 120 1 -0.5540567426063845 120 2 0.6337927500257976 120 3 -0.43697679732198225 120 4 0.40592428175254724 120 5 0.8811074962398635 120 6 0.2874859204441975 120 7 0.297555752597475 120 8 0.3990635856669513 120 9 0.7145645716150284 120 10 -0.19671218296426218 120 11 -3.255542525437722 120 12 -13.924812335756005 120 13 -12.406665556996131 120 14 -14.174281735226398 120 15 -8.772083820238208 120 16 -15.190281643553035 120 17 -13.93940906886473 120 18 -14.369271176539062 120 19 -7.202374751700262 120 20 -14.411061674163559 120 21 -3.759475150331177 120 22 -1.4534690578494853 120 23 -1.5755526592395412 120 24 -9.418298522361635 120 25 -2.114949218760259 120 26 1.503193210706229 120 27 1.2413592225123153 120 28 0.6374849073957236 120 29 2.3636982898998156 120 30 1.363212944166449 120 31 -0.2519867743704296 120 32 -8.144972476461724 120 33 0.5015826282950187 120 34 0.37989943758506683 120 35 0.7356318377491604 120 36 2.528761474027739 120 37 2.825743688180154 120 38 2.749584808856092 120 39 3.7549486666548546 120 40 7.760347324247618 120 41 3.145665299458635 120 42 -4.1597046016872845 120 43 -10.763815856992757 120 44 -9.507126653778641 120 45 -3.559687163898893 120 46 -14.31600494626541 120 47 -14.474805946900855 120 48 -8.562528811553776 120 49 -13.9485140906844 120 50 -8.037454337467548 120 51 -6.570077171083156 120 52 -6.387263737378884 120 53 -8.508879166287088 120 54 -13.73993655799754 120 55 -6.695346772011689 120 56 -4.4651757916112835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 276.0 5 55.0 6 9.0 7 38.0 8 78.0 9 74.0 10 49.0 11 40.0 12 30.0 13 46.0 14 58.0 15 64.0 16 81.0 17 89.0 18 122.0 19 134.0 20 213.0 21 243.0 22 323.0 23 361.0 24 413.0 25 499.0 26 528.0 27 656.0 28 745.0 29 942.0 30 1081.0 31 1373.0 32 1534.0 33 1633.0 34 2040.0 35 2344.0 36 2957.0 37 3657.0 38 3821.0 39 2547.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.55281207133059 1.1899862825788752 0.18175582990397804 0.07544581618655694 8 97.63031550068587 1.906721536351166 0.30178326474622774 0.16117969821673525 9 87.3079561042524 8.74485596707819 2.5308641975308643 1.416323731138546 10 31.375171467764062 45.579561042524006 12.170781893004115 10.87448559670782 11 29.10493827160494 33.573388203017835 19.58161865569273 17.7400548696845 12 36.546639231824415 18.20301783264746 20.75102880658436 24.49931412894376 13 23.56875793228827 19.394230439405895 26.909751998079102 30.12725963022673 14 22.90623499554153 24.497564990740106 27.26867412031003 25.327525893408325 15 28.235698333619652 20.70091049647827 24.569661570176944 26.493729599725135 16 26.692980950746524 23.929981122361422 24.053543847605972 25.32349407928608 17 24.739368998628258 26.574074074074073 21.347736625514404 27.33882030178326 18 22.360163242909564 28.642957577420354 22.521348468740353 26.47553071092973 19 24.64902344420417 28.991178388768752 22.850375862424055 23.509422304603028 20 24.194046760737457 26.971538435128917 24.321076664263398 24.51333813987022 21 24.818244170096023 23.127572016460906 26.069958847736626 25.98422496570645 22 25.64471879286694 24.859396433470508 24.773662551440328 24.72222222222222 23 25.70644718792867 26.13168724279835 26.330589849108364 21.83127572016461 24 23.196159122085046 27.458847736625515 27.4519890260631 21.89300411522634 25 24.489026063100138 23.988340192043896 31.20713305898491 20.315500685871058 26 20.918507339827137 23.03128001097544 34.840170119357936 21.210042529839484 27 22.02237935058694 19.544175190499075 37.87670762682776 20.55673783208622 28 19.493184081310304 20.78082615115201 40.85430759193764 18.87168217560004 29 17.21842944869464 19.71937287728567 44.43720196233147 18.62499571168822 30 16.66094950603732 19.226811196487375 47.049945115257955 17.062294182217343 31 14.697910591141456 18.924760695783444 50.461454008988916 15.915874704086184 32 16.22206200432588 17.080372163284924 51.37157963401655 15.325986198372643 33 14.687789958417815 16.540087288222963 54.342073610777 14.430049142582218 34 12.304365733113674 17.76153212520593 57.15952773201538 12.774574409665018 35 13.122855181880578 16.805078929306795 58.0885380919698 11.983527796842827 36 12.956354652758714 17.166483667307165 56.47474608838869 13.40241559154543 37 12.604406558278624 17.492180249544564 57.69429072285429 12.209122469322518 38 13.4812521499828 17.21362229102167 57.78465772273822 11.52046783625731 39 13.446995737659837 16.492506531005088 56.5378798295064 13.522617901828681 40 11.146056542475979 13.689776733254993 51.596737402364 23.567429321905024 41 9.702552027424773 13.764327019633644 47.931022542331796 28.602098410609784 42 12.992991617424762 16.916311666895698 57.54775319499794 12.542943520681598 43 11.882413615265602 14.602028537046587 56.57899260787348 16.93656523981434 44 11.452485103158475 16.767127062308408 53.973065132779944 17.80732270175318 45 13.411028602860286 17.615511551155116 57.0475797579758 11.925880088008801 46 13.123711340206185 18.498281786941583 55.518900343642606 12.85910652920962 47 11.780445969125214 19.159519725557463 55.914236706689536 13.145797598627787 48 11.854040681693238 20.636338647608575 55.82050577240242 11.689114898295767 49 10.514888337468982 18.262338020402535 48.54218362282878 22.680590019299697 50 11.414929064614752 21.36992889285837 55.168149496753806 12.046992545773076 51 13.0926983690042 21.46101438304315 54.15663065171014 11.289656596242516 52 13.030188549644537 21.44108252910671 53.30219459422331 12.226534327025448 53 11.980061876933654 22.99071845995187 53.509797181161915 11.519422481952562 54 11.581697548901646 23.015572896971364 49.895149369177346 15.507580184949637 55 13.299171166213847 23.26925061044812 52.25779825979296 11.173779963545071 56 13.178161314721859 23.77776249742144 51.578078800797634 11.465997387059067 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 7.5 7 15.0 8 30.0 9 45.0 10 64.5 11 84.0 12 103.0 13 148.5 14 175.0 15 204.0 16 233.0 17 317.0 18 401.0 19 520.0 20 639.0 21 700.0 22 852.5 23 944.0 24 1198.0 25 1452.0 26 1634.5 27 1817.0 28 2065.5 29 2351.5 30 2389.0 31 2153.0 32 1917.0 33 1670.0 34 1423.0 35 1178.5 36 934.0 37 759.0 38 499.0 39 414.0 40 459.5 41 505.0 42 482.5 43 460.0 44 431.5 45 403.0 46 513.0 47 612.0 48 601.0 49 616.0 50 631.0 51 653.0 52 675.0 53 738.5 54 837.0 55 872.0 56 908.5 57 945.0 58 931.0 59 917.0 60 992.5 61 1068.0 62 1008.0 63 854.5 64 761.0 65 696.5 66 632.0 67 581.0 68 530.0 69 454.5 70 379.0 71 372.5 72 256.5 73 147.0 74 136.0 75 125.0 76 96.5 77 68.0 78 47.5 79 16.5 80 6.0 81 4.5 82 3.0 83 3.0 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.02400548696844993 14 0.006858710562414267 15 0.1886145404663923 16 0.08573388203017833 17 0.0 18 0.0034293552812071334 19 0.0925925925925926 20 0.11316872427983539 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.013717421124828533 27 0.08916323731138547 28 0.12688614540466392 29 0.03772290809327847 30 0.027434842249657067 31 0.04458161865569273 32 0.11316872427983539 33 0.20919067215363513 34 0.0823045267489712 35 0.06858710562414265 36 0.054869684499314134 37 0.22976680384087794 38 0.30864197530864196 39 0.23319615912208505 40 0.7750342935528121 41 0.9636488340192043 42 0.17832647462277093 43 0.257201646090535 44 0.4355281207133059 45 0.24691358024691357 46 0.205761316872428 47 0.034293552812071325 48 0.19204389574759945 49 0.49382716049382713 50 0.16803840877914952 51 0.33607681755829905 52 0.1474622770919067 53 0.24005486968449932 54 0.24348422496570646 55 0.284636488340192 56 0.25377229080932784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 29160.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.73388203017832 #Duplication Level Percentage of deduplicated Percentage of total 1 86.30230030791523 65.36008230452674 2 7.91070458250317 11.982167352537724 3 2.476906357543923 5.627572016460905 4 1.181851113928636 3.580246913580247 5 0.6882811084948379 2.606310013717421 6 0.4347038579967397 1.9753086419753085 7 0.2218800941858359 1.1762688614540466 8 0.16754211193624344 1.0150891632373114 9 0.17207027712370948 1.1728395061728396 >10 0.43017569280927365 4.801097393689986 >50 0.013584495562398116 0.7030178326474623 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.24348422496570646 No Hit AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.23319615912208505 No Hit AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 66 0.22633744855967078 No Hit AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 45 0.15432098765432098 No Hit AGATGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG 37 0.12688614540466392 No Hit AGATGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG 34 0.11659807956104253 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.003429355281207133 0.0 0.0 0.0 0.0 10 0.0102880658436214 0.0 0.0 0.0 0.0 11 0.013717421124828532 0.0 0.0 0.0 0.0 12 0.013717421124828532 0.0 0.0 0.0 0.0 13 0.017146776406035666 0.0 0.0 0.0 0.0 14 0.017146776406035666 0.0 0.0 0.0 0.0 15 0.0205761316872428 0.0 0.0 0.0 0.0 16 0.030864197530864196 0.0 0.0 0.0 0.0 17 0.030864197530864196 0.0 0.0 0.0 0.0 18 0.03429355281207133 0.0 0.0 0.0 0.0 19 0.04801097393689986 0.0 0.0 0.0 0.0 20 0.05486968449931413 0.0 0.0 0.0 0.0 21 0.05486968449931413 0.0 0.0 0.0 0.0 22 0.06858710562414266 0.0 0.0 0.0 0.0 23 0.07544581618655692 0.0 0.0 0.0 0.0 24 0.09259259259259259 0.0 0.0 0.0 0.0 25 0.09945130315500686 0.0 0.0 0.0 0.0 26 0.10973936899862825 0.0 0.0 0.0 0.0 27 0.11316872427983539 0.0 0.0 0.0 0.0 28 0.12002743484224966 0.0 0.0 0.0 0.0 29 0.12002743484224966 0.0 0.0 0.0 0.0 30 0.12345679012345678 0.0 0.0 0.0 0.0 31 0.1337448559670782 0.0 0.0 0.0 0.0 32 0.1337448559670782 0.0 0.0 0.0 0.0 33 0.14060356652949246 0.0 0.0 0.0 0.0 34 0.14060356652949246 0.0 0.0 0.0 0.0 35 0.14060356652949246 0.0 0.0 0.0 0.0 36 0.14060356652949246 0.0 0.0 0.0 0.0 37 0.14746227709190673 0.0 0.0 0.0 0.0 38 0.14746227709190673 0.0 0.0 0.0 0.0 39 0.14746227709190673 0.0 0.0 0.0 0.0 40 0.14746227709190673 0.0 0.0 0.0 0.0 41 0.14746227709190673 0.0 0.0 0.0 0.0 42 0.14746227709190673 0.0 0.0 0.0 0.0 43 0.15089163237311384 0.0 0.0 0.0 0.0 44 0.15089163237311384 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAACC 30 9.704072E-7 50.308495 35 TGATATG 15 0.008204048 50.221455 50 CATTTGA 15 0.008260437 50.134716 46 TTGATAT 15 0.008260437 50.134716 49 AGCATTT 15 0.008317115 50.04828 44 AATGGCT 20 4.096714E-4 50.048275 29 GTTGATC 15 0.008374082 49.96214 16 TTCGTGG 25 2.0463262E-5 49.962135 15 ATTCGTG 25 2.0463262E-5 49.962135 14 ACAGCCA 15 0.008431338 49.87629 12 AATTCGT 20 4.1671286E-4 49.87629 13 TGGTAAA 15 0.008431338 49.87629 13 TGATCCT 15 0.008431338 49.87629 18 CAACAGC 15 0.008431338 49.87629 10 TGGAGAA 20 4.1671286E-4 49.87629 19 CATTATC 15 0.008431338 49.87629 10 AGAAATG 20 4.1671286E-4 49.87629 26 GAATTCG 20 4.1671286E-4 49.87629 12 GGGAATT 30 1.0302174E-6 49.87629 10 TACCTGG 15 0.008431338 49.87629 10 >>END_MODULE