FastQCFastQC Report
Fri 17 Jun 2016
SRR1526256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51144
Sequences flagged as poor quality0
Sequence length56
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG720.14077897700610043No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.12513686844986704No Hit
GTGGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG600.11731581417175035No Hit
GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA560.10949475989363366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAG150.00808888550.45643640
AACCAAC150.00808888550.45643641
CCACAGT150.00808888550.45643641
ACCAACA150.00837797450.01079642
CAACACA150.00837797450.01079644
TCCCTGG150.00837797450.01079648
TTTGATA150.00837797450.01079632
ACGGGTA650.049.9617733
CAAGAGT150.00841056349.96176514
CGCTTAC150.00841056349.96176513
AGAGTGT204.16178E-449.96176510
AATGGTA150.00841056349.96176510
TTGATAC150.00841056349.96176533
CTTAATC402.6138878E-949.96176515
AGCATGT252.0686144E-549.96176511
TCATCAT204.16178E-449.96176528
TGCAGGG150.00841056349.96176527
GTCAGTT150.00841056349.96176511
CGTCTTA402.6138878E-949.96176512
AAACCTC204.181988E-449.91283423
Produced by FastQC (version 0.11.5)