##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526256_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51144 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.37732676364774 38.0 36.0 38.0 33.0 39.0 2 35.15256921633036 38.0 34.0 38.0 28.0 39.0 3 35.782946191146564 38.0 35.0 38.0 29.0 39.0 4 35.52856640075082 38.0 35.0 38.0 29.0 39.0 5 32.91682308775223 35.0 31.0 37.0 23.0 39.0 6 34.27903566400751 38.0 35.0 39.0 18.0 39.0 7 36.359827154700454 38.0 36.0 39.0 31.0 39.0 8 36.34414594087283 38.0 36.0 39.0 31.0 39.0 9 36.265603003284845 38.0 36.0 39.0 31.0 39.0 10 35.56845377756922 38.0 35.0 39.0 29.0 39.0 11 35.13540200218989 38.0 33.0 39.0 28.0 40.0 12 33.07398717347098 37.0 31.0 39.0 19.0 39.0 13 32.70997575473174 36.0 30.0 39.0 18.0 39.0 14 32.965352729547945 37.0 31.0 39.0 19.0 39.0 15 32.93653214453308 37.0 31.0 39.0 19.0 39.0 16 32.99049741905209 36.0 31.0 38.0 20.0 39.0 17 32.96881354606601 36.0 30.0 38.0 20.0 39.0 18 32.310828249648054 36.0 29.0 38.0 18.0 39.0 19 33.2246597841389 36.0 31.0 38.0 23.0 39.0 20 33.297982167996246 36.0 31.0 38.0 23.0 39.0 21 33.9726067573909 37.0 33.0 38.0 24.0 39.0 22 33.697501173158145 36.0 32.0 38.0 23.0 39.0 23 34.16304942906304 37.0 33.0 38.0 25.0 39.0 24 33.75447755357422 37.0 32.0 38.0 23.0 39.0 25 34.021371030814954 37.0 33.0 38.0 24.0 39.0 26 34.65127874237447 38.0 34.0 39.0 27.0 39.0 27 34.63160879086501 38.0 34.0 39.0 27.0 39.0 28 34.58728296574378 38.0 33.0 39.0 27.0 39.0 29 34.48580478648522 38.0 33.0 39.0 26.0 39.0 30 34.367257156264664 38.0 33.0 39.0 26.0 39.0 31 34.67437040513061 38.0 34.0 39.0 27.0 39.0 32 34.296359299233536 38.0 33.0 39.0 25.0 39.0 33 34.443316909119346 38.0 34.0 39.0 27.0 39.0 34 34.43760753949633 38.0 34.0 39.0 27.0 39.0 35 34.28144063819803 38.0 33.0 39.0 26.0 39.0 36 33.69922180509933 36.0 33.0 38.0 26.0 39.0 37 33.41003832316596 36.0 32.0 38.0 25.0 39.0 38 33.07300954168622 36.0 32.0 38.0 23.0 39.0 39 32.66576724542468 35.0 31.0 38.0 21.0 39.0 40 29.420987799155327 35.0 24.0 38.0 8.0 39.0 41 26.0979978101048 31.0 14.0 38.0 5.0 39.0 42 30.255103237916472 34.0 28.0 38.0 8.0 39.0 43 28.918289535429377 33.0 26.0 38.0 4.0 39.0 44 28.831299859221023 33.0 25.0 38.0 4.0 39.0 45 29.892304082590332 34.0 28.0 38.0 4.0 39.0 46 29.589433755670264 35.0 27.0 38.0 4.0 39.0 47 29.04389566713593 35.0 26.0 38.0 4.0 39.0 48 29.152960269044268 35.0 26.0 38.0 4.0 39.0 49 26.69265211950571 33.0 15.0 38.0 4.0 39.0 50 28.692710777412795 34.0 25.0 38.0 4.0 39.0 51 28.91234553417801 35.0 26.0 39.0 4.0 39.0 52 28.684283591428123 35.0 25.0 39.0 4.0 39.0 53 28.273111215391836 35.0 23.0 38.0 4.0 39.0 54 27.33239480681996 34.0 17.0 38.0 4.0 39.0 55 27.590391834819332 34.0 19.0 38.0 4.0 39.0 56 26.515172845299546 33.0 16.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.2609964030611636 1 2 1.5918771677693186 1 3 1.9353889362643315 1 4 2.0268192770483964 1 5 1.4161087239904404 1 6 4.386013927933433 1 7 1.7966375830606793 1 8 1.7205723626820557 1 9 1.5923371098515204 1 10 1.016948111797923 1 11 -3.234298231579775 1 12 -10.387126293326794 1 13 -8.301701575045463 1 14 -5.357478703590356 1 15 -5.997172979661176 1 16 -23.265326543992792 1 17 -5.908467503452563 1 18 -14.807105111304566 1 19 -6.828359900333677 1 20 -6.519184308387231 1 21 -9.124624091367725 1 22 -5.240640106096809 1 23 -6.551044105244781 1 24 -6.545650712637393 1 25 -5.250254501479439 1 26 1.0272818731777633 1 27 1.559562314907751 1 28 1.3441795089301891 1 29 1.5326535343038188 1 30 2.583299696460685 1 31 2.0888222379560446 1 32 1.7511376046834215 1 33 1.4544606717834796 1 34 1.6072599786770425 1 35 1.2752638756989256 1 36 1.8930148436502208 1 37 1.4344225844461107 1 38 2.673704638291845 1 39 2.1400156899951774 1 40 4.425680835823968 1 41 -11.881661007148695 1 42 -13.24746220818554 1 43 -18.33809358113743 1 44 -21.579153513672047 1 45 -15.323763094397208 1 46 -18.043508012191673 1 47 -22.258768707118932 1 48 -16.5961536387783 1 49 -19.65891461587031 1 50 -15.919634255878977 1 51 -13.520999220668623 1 52 -13.218931351787074 1 53 -12.916430091046983 1 54 -12.023964869609635 1 55 -12.445466465194507 1 56 -9.308439632828478 2 1 1.8373898456841147 2 2 2.448270610392271 2 3 2.5045692641331883 2 4 2.626600697813423 2 5 1.838185226722679 2 6 5.088746168370591 2 7 2.323194960109859 2 8 2.3885505047585553 2 9 2.330916344824196 2 10 2.2403360899399942 2 11 1.9073411126825235 2 12 -1.9003503370426422 2 13 -1.0468381870673227 2 14 3.610171569633689 2 15 3.10708931542079 2 16 2.2913947674826147 2 17 3.4218057205911485 2 18 -2.6713674063865334 2 19 1.991530810048836 2 20 2.153383997623699 2 21 2.654829460544846 2 22 2.70449650592505 2 23 2.0845843100557673 2 24 0.925387538728728 2 25 2.1311662635478825 2 26 2.6978829660739407 2 27 2.7702726974214116 2 28 2.8777314214984955 2 29 3.291779217363924 2 30 2.589638494274894 2 31 1.8376746969724422 2 32 2.6849080964867014 2 33 1.8660453712370284 2 34 2.081576918567748 2 35 2.3377775369011147 2 36 2.213014843650221 2 37 2.4407613822603196 2 38 2.567037971625176 2 39 3.5347697883558347 2 40 5.30109067188954 2 41 -4.513136416984761 2 42 -5.489648000535269 2 43 -11.57656352649262 2 44 -13.96057427869937 2 45 -5.6883532583316345 2 46 -9.2080981761261 2 47 -17.491774171599808 2 48 -7.313530687958629 2 49 -11.548313522974135 2 50 -6.821492179376246 2 51 -0.6930210785921211 2 52 -0.8021007506941764 2 53 -0.31096561017266566 2 54 0.11658157847779549 2 55 -0.10688723022182955 2 56 2.5591013507780787 3 1 2.06686352989464 3 2 2.6872179788133224 3 3 2.8529903167647674 3 4 2.7136182416730747 3 5 2.4704659284770685 3 6 5.169447922756554 3 7 2.534773907478275 3 8 2.7313575223024174 3 9 2.609161958859282 3 10 2.476827318010173 3 11 3.259446375840419 3 12 4.953684750676654 3 13 4.035442514687066 3 14 4.446662797703862 3 15 3.7812998417365797 3 16 4.2077105569563 3 17 4.49619168550343 3 18 5.624948383087151 3 19 3.8571448451365598 3 20 4.463559436220187 3 21 3.618689109667649 3 22 3.9090579094338196 3 23 3.30335623988033 3 24 3.5999489422374964 3 25 3.5750259126706894 3 26 2.770163667828328 3 27 3.156413048298603 3 28 1.7231700179897231 3 29 2.6745862349077854 3 30 2.284550774976644 3 31 2.3655694338145423 3 32 2.6143817806972294 3 33 0.9225365993072074 3 34 2.0824541115502058 3 35 1.9954968351467244 3 36 2.1035411594396933 3 37 3.0435683998041796 3 38 2.7131783225023725 3 39 3.4784539988821486 3 40 6.7958275139948014 3 41 8.346337267225767 3 42 4.370527438061224 3 43 -0.3825284387733241 3 44 2.5955660721778244 3 45 4.467436215352578 3 46 3.5371649817686404 3 47 -2.7494934698454223 3 48 2.688574575199265 3 49 5.386598757727619 3 50 3.0760516802728795 3 51 4.568733307372796 3 52 4.634390477375998 3 53 4.308332635441371 3 54 5.0485114030392 3 55 5.137147857497467 3 56 5.720680298146501 4 1 1.5787597086978096 4 2 1.4849829391593943 4 3 2.477719949064692 4 4 2.2399340311467597 4 5 1.4394637655354643 4 6 4.619339775676529 4 7 1.6223730423016391 4 8 2.1953084956261364 4 9 1.87484328546347 4 10 1.652025587656894 4 11 3.105697277066085 4 12 4.622754685788404 4 13 4.685947201060532 4 14 5.046518601597153 4 15 5.045719452407095 4 16 4.8492029866607 4 17 4.458700697760104 4 18 4.90342711416141 4 19 3.942581038359343 4 20 3.5486351391762057 4 21 1.7711764925083102 4 22 2.415546734235555 4 23 1.4235797438457212 4 24 2.5482185889570346 4 25 1.98778726811409 4 26 1.7254628747497378 4 27 1.1854325147730052 4 28 1.5694209192153892 4 29 1.6179892630260255 4 30 1.444067718019184 4 31 0.9987705873833974 4 32 1.1194286444319062 4 33 1.2415704853922804 4 34 0.4222161879741435 4 35 1.0915674912390116 4 36 1.5916449806365236 4 37 1.0486152635388564 4 38 1.5915128574699295 4 39 1.336961569177749 4 40 5.818898891067626 4 41 7.217822487124831 4 42 3.8151921821131367 4 43 5.15640451003706 4 44 4.826640333172776 4 45 4.014660440298499 4 46 3.075737440312256 4 47 3.236627654884213 4 48 3.4355104079317833 4 49 6.299540358304402 4 50 3.939786359436539 4 51 2.5701752684398436 4 52 3.247944911406279 4 53 2.2762490016994796 4 54 4.792563313637615 4 55 2.5767657378147 4 56 3.113073953517805 5 1 2.0993076539032884 5 2 3.019229514501859 5 3 2.7927884422153753 5 4 3.007057318817992 5 5 3.0832993819738235 5 6 5.304271282525839 5 7 2.4305922203838293 5 8 2.5377742490507913 5 9 2.354295340257991 5 10 2.3506557246431967 5 11 3.3796698798058102 5 12 6.1022067405829254 5 13 5.576358159964638 5 14 5.0054227111861955 5 15 5.730650959256412 5 16 5.493038603099052 5 17 5.883358232006675 5 18 6.369180538818945 5 19 4.367238572605913 5 20 4.877402262463882 5 21 3.798573752782289 5 22 3.388149473961583 5 23 3.437278373982707 5 24 4.603013109504985 5 25 2.6179242544154633 5 26 2.670668354201794 5 27 1.71967909011547 5 28 2.0214757137359385 5 29 2.165934468505476 5 30 2.2248896358274024 5 31 2.259044559986137 5 32 2.6810724800483428 5 33 2.0360910333374918 5 34 2.0797504345494886 5 35 2.351841463841751 5 36 3.2628778573488475 5 37 3.952724852579955 5 38 3.523019706784993 5 39 4.789016363698298 5 40 8.627118069149816 5 41 11.272617007672775 5 42 7.5275209492364255 5 43 8.320788071680898 5 44 7.854037593446748 5 45 6.233838522490281 5 46 6.322312782778013 5 47 6.428408476802019 5 48 5.9834556134112376 5 49 9.381732139126324 5 50 6.734306907381743 5 51 6.4879834876179245 5 52 6.152054500447374 5 53 6.550221604439205 5 54 6.778864683500625 5 55 5.837039710417443 5 56 5.332252035709587 6 1 1.6009192574488154 6 2 1.5641529633334414 6 3 2.058098675897888 6 4 2.0634634429114627 6 5 2.3915185600560136 6 6 4.69266773699804 6 7 2.0061361365804444 6 8 1.69188383809189 6 9 1.9922888938438064 6 10 1.6548458938615624 6 11 3.3879293479766375 6 12 5.741610447271082 6 13 6.000612793324837 6 14 4.6687990206140775 6 15 4.876501080126676 6 16 4.916688885129673 6 17 4.8251390539244845 6 18 5.05451494655464 6 19 3.704668064950802 6 20 3.9620114486040876 6 21 3.7022804409370025 6 22 4.015869054944659 6 23 2.8141921531930194 6 24 3.3097012642189227 6 25 2.4527348909988618 6 26 2.514745711171976 6 27 1.7051746582057206 6 28 1.6310647548318258 6 29 1.870406668344316 6 30 2.3147365334905814 6 31 1.6635570499136136 6 32 1.648437508251405 6 33 1.615261057511539 6 34 1.3541259381755921 6 35 1.3391500859207213 6 36 2.290073667179634 6 37 1.9305653038289492 6 38 2.908998755938903 6 39 2.6518286118852465 6 40 6.121678907183657 6 41 2.600981230074062 6 42 4.898391215151008 6 43 5.420103140174046 6 44 4.423739446790826 6 45 3.7398820357860103 6 46 4.2995488826974295 6 47 5.142147397027642 6 48 3.6258810767472553 6 49 6.196196280947433 6 50 4.421252918662972 6 51 3.9944299018000358 6 52 3.6212325826391556 6 53 3.6380539976704718 6 54 5.26677765690917 6 55 4.160367671738953 6 56 3.824395468425138 7 1 1.6523898456841124 7 2 2.3426456103922675 7 3 2.437694264133185 7 4 2.3493090311467597 7 5 1.5321435600560136 7 6 5.362704501703924 7 7 2.3094449601098574 7 8 2.37938383809189 7 9 2.3213330114908644 7 10 2.1824194232733305 7 11 3.4017161126825215 7 12 5.490691329624024 7 13 5.206495146266011 7 14 5.6678799029670195 7 15 5.737714315420792 7 16 4.884519767482615 7 17 4.8563890539244845 7 18 5.661132593613466 7 19 4.996947476715505 7 20 4.35171733095703 7 21 3.0175377938781764 7 22 3.313663172591717 7 23 2.2425009767224324 7 24 3.2701792053953938 7 25 3.176999596881217 7 26 3.144341299407273 7 27 2.6169393640880756 7 28 1.7873147548318258 7 29 3.556987550697258 7 30 2.7154718276082264 7 31 2.4732996969724397 7 32 3.231158096486702 7 33 2.440628704570365 7 34 2.163868585234418 7 35 2.7931942035677793 7 36 2.5961398436502208 7 37 1.9259697155936522 7 38 2.658079638291845 7 39 3.5203947883558335 7 40 6.994840671889541 7 41 1.1662385830152395 7 42 4.524310332798066 7 43 6.263853140174046 7 44 5.977967387967297 7 45 4.386021741668365 7 46 5.2444018238739005 7 47 5.249684161733526 7 48 6.018344312041371 7 49 8.770644810359197 7 50 6.048091153957088 7 51 5.965937254741213 7 52 6.480607582639156 7 53 6.505701056494001 7 54 6.4901232451444635 7 55 5.142904436444837 7 56 6.74535135077808 8 1 2.045505787713097 8 2 2.738705393000963 8 3 2.7816707134085448 8 4 2.7181949007119783 8 5 3.0074605890415214 8 6 5.410485298805376 8 7 2.7944993079359435 8 8 2.7317389105556558 8 9 2.4341047506213016 8 10 2.662944785592174 8 11 3.14560198224774 8 12 5.368635170203735 8 13 5.365915436121082 8 14 5.226983163836582 8 15 5.419553083536737 8 16 4.9603802747289905 8 17 4.031660793054922 8 18 5.616748535642451 8 19 4.763704723092317 8 20 4.291934722261381 8 21 3.6472932286607858 8 22 3.9902936073743263 8 23 3.2764683680267837 8 24 4.163068698149019 8 25 4.183340176591365 8 26 2.8578829660739373 8 27 3.0589683495953253 8 28 2.4209198272955916 8 29 3.2836632753349377 8 30 3.1565950160140233 8 31 1.8272399143637443 8 32 2.614763168950468 8 33 2.043436675584857 8 34 2.046794309872098 8 35 2.078067391973576 8 36 2.240840930606744 8 37 2.225109208347277 8 38 2.3693568122048916 8 39 3.7620161651674273 8 40 5.959061686382292 8 41 -0.729368301042733 8 42 3.4923809849719802 8 43 5.0396683575653505 8 44 4.914788040141211 8 45 4.22411050978431 8 46 5.475380084743463 8 47 6.26938524869005 8 48 6.194295398997895 8 49 8.374585027750502 8 50 6.01502955975419 8 51 6.243790515610776 8 52 5.211812292784085 8 53 5.4803387376534225 8 54 5.9432482451444635 8 55 5.352822914705708 8 56 6.433304249328806 9 1 2.1430614874751583 9 2 2.4888676253176385 9 3 2.477106577566019 9 4 2.4787400012960106 9 5 3.1452499033395966 9 6 5.177303382300941 9 7 2.343493467572543 9 8 2.352331599285918 9 9 2.1044486831326523 9 10 2.4524753934225814 9 11 2.965848575369087 9 12 5.454077523653876 9 13 5.3706742507436225 9 14 5.840221320877465 9 15 5.481417673629746 9 16 4.543335065990078 9 17 5.284094277805078 9 18 5.400871399583615 9 19 4.837898969252819 9 20 4.308806883195835 9 21 3.609655331191611 9 22 3.2213124263230597 9 23 3.4873703797075066 9 24 3.624890026290913 9 25 2.4316637759856974 9 26 1.5344998814968278 9 27 1.1020139909537505 9 28 2.6982289339363064 9 29 2.146306580548007 9 30 2.343270335070912 9 31 1.4800627566739308 9 32 1.3099827233523769 9 33 0.9504234806897713 9 34 0.4616764210553157 9 35 0.9662352483438994 9 36 1.669134246635295 9 37 2.20931859619067 9 38 2.5990777726202055 9 39 3.4786503853707593 9 40 4.438404104725365 9 41 2.568057612865985 9 42 2.8687599596637376 9 43 4.692491199875537 9 44 4.440420746176251 9 45 2.9277661446534395 9 46 4.625931674620169 9 47 4.802156176658897 9 48 3.771842446369728 9 49 7.1468108551353176 9 50 3.720199362912311 9 51 3.9316555383233016 9 52 3.9085460154749754 9 53 3.6362980714193753 9 54 4.823845260069838 9 55 4.036794361817972 9 56 5.257011798539274 10 1 1.9870672650389523 10 2 2.269560932972915 10 3 2.452311199617057 10 4 2.288321127920952 10 5 2.6495830761850456 10 6 4.964014985574892 10 7 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114 39 1.776508918790615 114 40 4.9663080631938925 114 41 2.9155592351891535 114 42 -1.0293581454628047 114 43 -5.742940338086825 114 44 -7.954777177250094 114 45 -1.3266141278968533 114 46 -6.3724460022130565 114 47 -6.404527794788212 114 48 -5.849182861871672 114 49 -17.88628453746689 114 50 -6.93424580256465 114 51 -6.49533991917183 114 52 -5.367897852143454 114 53 -7.352994595679913 114 54 -9.774143059203361 114 55 -4.929785780946467 114 56 -1.5087247361784435 115 1 1.6173898456841158 115 2 1.768270610392264 115 3 2.1445692641331817 115 4 2.1199340311467623 115 5 1.971518560056012 115 6 4.662079501703921 115 7 1.8431949601098552 115 8 1.9018838380918908 115 9 1.4375830114908652 115 10 1.553669423273334 115 11 -1.1126588873174796 115 12 -11.433683670375977 115 13 -7.7135048537339905 115 14 -7.4364950970329815 115 15 -8.632910684579208 115 16 -7.988605232517386 115 17 -2.984860946075514 115 18 -2.5713674063865355 115 19 -4.0818025232844946 115 20 -3.253282669042971 115 21 2.6881627938781776 115 22 2.3711631725917144 115 23 2.4312509767224313 115 24 2.972054205395395 115 25 3.0644995968812196 115 26 1.8912162994072759 115 27 1.8569393640880776 115 28 1.6110647548318227 115 29 2.0451125506972545 115 30 2.1629718276082244 115 31 1.9776746969724428 115 32 2.024908096486705 115 33 1.919378704570363 115 34 2.1882435852344173 115 35 1.8044442035677761 115 36 2.43301484365022 115 37 2.3940947155936527 115 38 3.0137046382918484 115 39 2.5547697883558307 115 40 5.721090671889542 115 41 6.366863583015238 115 42 1.6636853327980674 115 43 -5.349896859825954 115 44 -7.113907612032705 115 45 0.8516467416683646 115 46 -3.3880981761260998 115 47 -3.4184408382664735 115 48 -2.8735306879586275 115 49 -13.154980189640803 115 50 -3.808158846042911 115 51 -2.9396877452587873 115 52 -2.688767417360843 115 53 -3.164298943506001 115 54 -6.6767517548555375 115 55 -2.720220563555163 115 56 -0.5208986492219196 116 1 2.1567001905116996 116 2 2.642753369012958 116 3 2.553534781374566 116 4 2.5847616173536565 116 5 3.6156564910904976 116 6 5.170355363772892 116 7 2.3342294428684767 116 8 2.269470044988445 116 9 2.391376114939142 116 10 2.5067728715491953 116 11 -1.4016244045588593 116 12 -13.424718153134595 116 13 -8.431435888216747 116 14 -3.6958054418605677 116 15 -6.290841719061966 116 16 -4.62032937044842 116 17 -2.87313680814448 116 18 -7.341022578800327 116 19 -4.325250799146563 116 20 -4.23879991042228 116 21 3.3564386559471444 116 22 2.475301103626201 116 23 2.4181475284465677 116 24 3.3817093778091873 116 25 1.9162237348122488 116 26 2.7256990580279634 116 27 2.1341807433984172 116 28 0.8896854444870002 116 29 1.385112550697258 116 30 2.316764931056504 116 31 0.8887091797310589 116 32 1.7904253378660115 116 33 1.7455856011220874 116 34 0.5103125507516566 116 35 0.20375454839536644 116 36 2.0826700160640144 116 37 1.9609912673177874 116 38 2.277152914153916 116 39 3.3064939262868656 116 40 6.435573430510232 116 41 4.134449789911791 116 42 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1.8502726974214099 119 28 1.4199536437207172 119 29 1.940668106252815 119 30 0.7807496053860064 119 31 0.04656358586132825 119 32 -4.163980792402185 119 33 -0.06284351765185647 119 34 0.281576918567751 119 35 -0.4755557964322179 119 36 0.8930148436502208 119 37 1.4763169378158736 119 38 0.8514824160696222 119 39 1.9658808994669421 119 40 4.078868449667318 119 41 1.2290858052374602 119 42 -5.294092444979711 119 43 -10.829896859825954 119 44 -11.18279650092159 119 45 -5.759464369442746 119 46 -16.323653731681656 119 47 -15.589551949377585 119 48 -11.420197354625294 119 49 -13.050535745196358 119 50 -9.497047734931801 119 51 -8.288576634147674 119 52 -8.073211861805287 119 53 -8.599854499061554 119 54 -12.234529532633314 119 55 -7.804665007999606 119 56 -4.0497875381108095 120 1 1.0652686335629014 120 2 1.7900887922104474 120 3 1.8330541126180364 120 4 1.9369037281164552 120 5 2.4975791661166227 120 6 4.100867380491799 120 7 1.2213767782916776 120 8 1.3812777774858276 120 9 1.416976950884802 120 10 1.5773057869096974 120 11 -3.817507372165963 120 12 -13.630047306739613 120 13 -13.308656368885504 120 14 -14.392252672790555 120 15 -13.743819775488298 120 16 -16.76557492948708 120 17 -14.682436703651273 120 18 -17.605912860931987 120 19 -9.676347977829948 120 20 -11.939949335709638 120 21 -5.334261448546066 120 22 -2.960958039529494 120 23 -2.470567205095751 120 24 -7.444309430968243 120 25 -2.68095494857333 120 26 0.5548526630436399 120 27 0.14724239439110676 120 28 0.10076172452879462 120 29 0.02147618706089105 120 30 0.6453960700324686 120 31 -1.4059616666639272 120 32 -11.70539493381633 120 33 -1.3557728105811506 120 34 -1.2456958087049728 120 35 -1.342222463098885 120 36 -0.44031848968311493 120 37 0.011670473169409945 120 38 0.12825009283729827 120 39 1.0729516065376536 120 40 4.3556361264349945 120 41 -2.738590962439307 120 42 -6.84358739447466 120 43 -13.193533223462317 120 44 -13.45148336960846 120 45 -9.393807803786181 120 46 -17.113552721580646 120 47 -16.532986292811927 120 48 -13.789894324322265 120 49 -17.525283219943834 120 50 -13.729370967255033 120 51 -12.254233199804242 120 52 -12.356040144633571 120 53 -12.335208034415091 120 54 -13.056751754855537 120 55 -11.529917533252132 120 56 -8.463928952252225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 411.0 5 16.0 6 47.0 7 59.0 8 72.0 9 74.0 10 68.0 11 52.0 12 65.0 13 76.0 14 112.0 15 168.0 16 190.0 17 268.0 18 291.0 19 394.0 20 460.0 21 636.0 22 708.0 23 865.0 24 1010.0 25 1077.0 26 1228.0 27 1329.0 28 1584.0 29 1837.0 30 2203.0 31 2549.0 32 2908.0 33 3271.0 34 4011.0 35 4761.0 36 5973.0 37 6473.0 38 4440.0 39 1458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.45448146410136 0.3304395432504301 0.19748162052244642 0.017597372125762553 8 99.22767089003598 0.6276396058188644 0.0645236977944627 0.08016580635069608 9 96.88135460660096 2.0354293758798687 0.5728922258720476 0.5103237916471141 10 39.861958391991244 43.35601439073987 8.906225559205382 7.875801658063507 11 28.294619114656655 34.38722039730956 21.519630846238073 15.798529641795714 12 39.22063194118567 15.890427029563586 21.159862349444705 23.729078679806037 13 22.53793804755945 19.28387046307885 26.533166458072593 31.64502503128911 14 26.111100248323325 21.760553741469995 26.191267622157483 25.937078388049194 15 27.239581291410204 20.415964245109187 26.073234798290667 26.27121966518995 16 27.654205058982335 21.70315159340337 26.832560596279126 23.81008275133517 17 31.40348819020804 21.69364930392617 21.9439230408259 24.958939465039887 18 27.502737368997344 23.195291725324573 23.86203660253402 25.439934303144064 19 25.9601275605032 26.678144503355313 21.565942128225696 25.795785807915795 20 26.888832358178583 25.387941999491225 21.93021936089858 25.79300628143162 21 28.11277960269044 21.78359142812451 26.354997653683714 23.748631315501328 22 29.704364148287187 22.68301267010793 23.257860159549505 24.354763022055373 23 29.710229938995774 24.260910370717973 24.64414203034569 21.38471765994056 24 27.1356783919598 25.99182683847252 24.71110415892693 22.16139061064075 25 27.301879045030603 26.52757953190075 25.127583442504353 21.0429579805643 26 26.615694479751266 24.05991513326424 28.870333796124285 20.454056590860205 27 26.50281778334377 22.285926737633062 29.119051346274265 22.092204132748904 28 24.948627120966005 23.114859972209718 31.921639235180148 20.01487367164413 29 24.327178845251137 23.779533719292758 32.712016898763885 19.181270536692224 30 21.883678178902493 22.11053310908593 36.85805921695936 19.147729495052214 31 21.523146427803095 22.592947526940605 37.576030196945105 18.307875848311202 32 21.287632619504365 21.70849156324629 39.523548526014956 17.480327291234392 33 20.002349762086588 22.393232685190625 40.48640075192387 17.11801680079892 34 17.827379787941624 22.242654250948785 43.52282953167182 16.40713642943777 35 19.329407852266282 22.445665995031202 44.32794068741564 13.896985465286878 36 19.589665356255743 22.603610475464023 42.617692503276025 15.189031665004205 37 19.557809807300643 21.68063606454645 43.6981043396522 15.063449788500705 38 18.115189402104686 23.053557780869703 44.30029982950871 14.530952987516901 39 18.530420299794258 23.438816498481437 42.54531204075634 15.485451160967964 40 13.664376144440727 17.836539408139558 39.06751461930733 29.431569828112387 41 13.849765258215962 16.228745019134415 36.25478360358228 33.66670611906735 42 16.689182574645127 22.053842388644153 44.25844346549192 16.998531571218795 43 14.801097393689986 20.096021947873798 43.78012933568489 21.322751322751323 44 14.956484155517574 20.87581776389462 42.058112807214 22.109585273373806 45 16.641203431660596 24.051004818427547 44.61158773063815 14.69620401927371 46 17.55544236345114 23.246610767181256 44.44009090196693 14.757855967400674 47 16.34557083341483 21.919924892913727 45.15422379564614 16.58028047802531 48 16.822045516863174 23.208899682713778 45.981041168866774 13.98801363155627 49 14.344930554191304 19.556804117635505 39.757971042964066 26.340294285209126 50 14.612944907788089 24.180664865499825 45.61650808567289 15.589882141039196 51 17.93534727803807 23.904648017094352 43.12795279449531 15.032051910372275 52 16.841899167890357 25.797356828193834 42.77239353891336 14.588350465002447 53 16.835095965530748 25.63846455150803 43.01605953779867 14.510379945162555 54 14.755287245927986 23.993022207412924 41.39830259315157 19.853387953507518 55 15.925214596480226 24.99902010739623 42.91145690432329 16.164308391800258 56 18.054848188050933 25.951028403525957 41.05974534769833 14.934378060724779 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.5 9 5.0 10 18.5 11 32.0 12 72.0 13 159.0 14 206.0 15 228.0 16 250.0 17 334.5 18 419.0 19 519.5 20 620.0 21 684.0 22 914.5 23 1081.0 24 1316.5 25 1552.0 26 1911.0 27 2270.0 28 2512.5 29 2853.0 30 2951.0 31 2890.5 32 2830.0 33 2507.0 34 2184.0 35 1867.0 36 1550.0 37 1357.0 38 1068.5 39 973.0 40 961.0 41 949.0 42 974.5 43 1000.0 44 974.0 45 948.0 46 1012.5 47 1165.0 48 1253.0 49 1428.5 50 1604.0 51 1653.0 52 1702.0 53 1792.5 54 1932.0 55 1981.0 56 2036.0 57 2091.0 58 2173.0 59 2255.0 60 2351.5 61 2448.0 62 2348.5 63 2126.5 64 2004.0 65 1871.0 66 1738.0 67 1508.0 68 1278.0 69 1129.5 70 981.0 71 847.5 72 609.5 73 505.0 74 448.0 75 391.0 76 298.0 77 205.0 78 155.0 79 78.5 80 52.0 81 33.0 82 14.0 83 12.5 84 11.0 85 7.5 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01564210855623338 14 0.0019552635695291726 15 0.25418426403879246 16 0.05279211637728765 17 0.0 18 0.0 19 0.06061317065540434 20 0.08016580635069608 21 0.0 22 0.0 23 0.0 24 0.0019552635695291726 25 0.0019552635695291726 26 0.009776317847645863 27 0.0782105427811669 28 0.09189738776787111 29 0.03128421711246676 30 0.019552635695291726 31 0.025418426403879243 32 0.11340528703269201 33 0.14664476771468793 34 0.05083685280775849 35 0.048881589238229314 36 0.02932895354293759 37 0.1564210855623338 38 0.22485531049585483 39 0.2131237290786798 40 0.6941185671828562 41 0.8798686062881277 42 0.1349131862975129 43 0.22290004692632567 44 0.4751290473955889 45 0.17597372125762553 46 0.19552635695291726 47 0.033239480681995934 48 0.16815266697950884 49 0.47121852025653055 50 0.1290473955889254 51 0.2600500547473799 52 0.1349131862975129 53 0.1642421398404505 54 0.24440794619114659 55 0.23072110120444236 56 0.18379477553574222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 51144.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.90442671672142 #Duplication Level Percentage of deduplicated Percentage of total 1 87.06905318824367 66.95995620209604 2 7.467202278043324 11.48521820741436 3 2.555171361741076 5.895119662130456 4 1.0652903488253838 3.277021742530893 5 0.5415437811451236 2.0823557015485687 6 0.37374148276212754 1.7245424683247301 7 0.2237363978439947 1.2044423588299702 8 0.15254754398454184 0.9385265133740028 9 0.11949557612122444 0.82707648991084 >10 0.4220482050238991 5.113014234318786 >50 0.010169836265636122 0.49272641952135143 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 72 0.14077897700610043 No Hit GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64 0.12513686844986704 No Hit GTGGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 60 0.11731581417175035 No Hit GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 56 0.10949475989363366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.01564210855623338 0.0 0.0 0.0 0.0 7 0.01564210855623338 0.0 0.0 0.0 0.0 8 0.01564210855623338 0.0 0.0 0.0 0.0 9 0.01564210855623338 0.0 0.0 0.0 0.0 10 0.0215078992648209 0.0 0.0 0.0 0.0 11 0.02346316283435007 0.0 0.0 0.0 0.0 12 0.03128421711246676 0.0 0.0 0.0 0.0 13 0.035194744251525106 0.0 0.0 0.0 0.0 14 0.041060534960112624 0.0 0.0 0.0 0.0 15 0.05083685280775849 0.0 0.0 0.0 0.0 16 0.05083685280775849 0.0 0.0 0.0 0.0 17 0.05279211637728766 0.0 0.0 0.0 0.0 18 0.05474737994681683 0.0 0.0 0.0 0.0 19 0.06256843422493352 0.0 0.0 0.0 0.0 20 0.07038948850305021 0.0 0.0 0.0 0.0 21 0.07430001564210856 0.0 0.0 0.0 0.0 22 0.08212106992022525 0.0 0.0 0.0 0.0 23 0.09189738776787111 0.0 0.0 0.0 0.0 24 0.09776317847645863 0.0 0.0 0.0 0.0 25 0.10558423275457532 0.0 0.0 0.0 0.0 26 0.11536055060222118 0.0 0.0 0.0 0.0 27 0.11536055060222118 0.0 0.0 0.0 0.0 28 0.1270921320193962 0.0 0.0 0.0 0.0 29 0.1349131862975129 0.0 0.0 0.0 0.0 30 0.14273424057562958 0.0 0.0 0.0 0.0 31 0.14664476771468793 0.0 0.0 0.0 0.0 32 0.14860003128421712 0.0 0.0 0.0 0.0 33 0.15055529485374627 0.0 0.0 0.0 0.0 34 0.15446582199280462 0.0 0.0 0.0 0.0 35 0.15837634913186296 0.0 0.0 0.0 0.0 36 0.15837634913186296 0.0 0.0 0.0 0.0 37 0.15837634913186296 0.0 0.0 0.0 0.0 38 0.1622868762709213 0.0 0.0 0.0 0.0 39 0.16815266697950884 0.0 0.0 0.0 0.0 40 0.170107930549038 0.0 0.0 0.0 0.0 41 0.17597372125762553 0.0 0.0 0.0 0.0 42 0.18770530267480057 0.0 0.0 0.0 0.0 43 0.19357109338338807 0.0 0.0 0.0 0.0 44 0.1994368840919756 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCACAG 15 0.008088885 50.456436 40 AACCAAC 15 0.008088885 50.456436 41 CCACAGT 15 0.008088885 50.456436 41 ACCAACA 15 0.008377974 50.010796 42 CAACACA 15 0.008377974 50.010796 44 TCCCTGG 15 0.008377974 50.010796 48 TTTGATA 15 0.008377974 50.010796 32 ACGGGTA 65 0.0 49.96177 33 CAAGAGT 15 0.008410563 49.961765 14 CGCTTAC 15 0.008410563 49.961765 13 AGAGTGT 20 4.16178E-4 49.961765 10 AATGGTA 15 0.008410563 49.961765 10 TTGATAC 15 0.008410563 49.961765 33 CTTAATC 40 2.6138878E-9 49.961765 15 AGCATGT 25 2.0686144E-5 49.961765 11 TCATCAT 20 4.16178E-4 49.961765 28 TGCAGGG 15 0.008410563 49.961765 27 GTCAGTT 15 0.008410563 49.961765 11 CGTCTTA 40 2.6138878E-9 49.961765 12 AAACCTC 20 4.181988E-4 49.912834 23 >>END_MODULE