FastQCFastQC Report
Fri 17 Jun 2016
SRR1526255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125966
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5430.43106870107806866No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4080.32389692456694663No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC3980.3159582744550117No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3970.3151644094438182No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1610.12781226680215296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGG150.00813215350.43202237
GTAAGCG150.00817103450.37123535
GGTAAGC150.00826229350.2299734
TTGTGAC150.008275391550.20985447
TGCAATA150.008275391550.20985447
CGATTAA301.0135009E-650.1897549
AATGCAA150.00830163550.1696745
GCCCCGT150.00844708549.94980211
AGTCCAA150.00844708549.94980211
GTCTACG301.0475942E-649.94980210
TACGCTT150.00844708549.94980212
GTCGTTC150.00844708549.94980211
CATACAC150.00844708549.94980212
GAGGCAT150.00844708549.94980210
AGCTTAT204.1902656E-449.949811
TTAAGCC204.1902656E-449.949813
GAGAGTA252.0940684E-549.929919
GAAGTTT150.00846040149.929919
GATTACG150.00846040149.929919
GACAGTA204.1985148E-449.9299059