##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526255_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 125966 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.460846577647935 39.0 38.0 39.0 34.0 40.0 2 36.608942095486086 39.0 38.0 39.0 31.0 40.0 3 37.071773335662 39.0 38.0 39.0 33.0 40.0 4 36.32222186939333 39.0 38.0 39.0 30.0 40.0 5 36.87914992934601 39.0 38.0 39.0 33.0 40.0 6 36.05304606004795 39.0 36.0 39.0 30.0 40.0 7 36.6775955416541 39.0 38.0 39.0 32.0 40.0 8 36.51866376641316 39.0 37.0 39.0 31.0 40.0 9 36.462362859819315 39.0 37.0 39.0 31.0 40.0 10 35.9448581363225 39.0 35.0 39.0 29.0 40.0 11 35.334193353762124 38.0 34.0 39.0 29.0 40.0 12 33.35890637156058 37.0 32.0 39.0 19.0 40.0 13 33.307646507787815 37.0 32.0 39.0 19.0 40.0 14 33.60166235333344 38.0 32.0 39.0 20.0 40.0 15 33.49118809837575 38.0 32.0 39.0 19.0 40.0 16 32.8164822253624 37.0 30.0 39.0 18.0 40.0 17 32.8553260403601 36.0 30.0 39.0 20.0 40.0 18 32.33864693647492 36.0 30.0 39.0 17.0 40.0 19 33.191996252957146 36.0 31.0 39.0 23.0 40.0 20 32.96758649159297 36.0 30.0 39.0 21.0 40.0 21 33.80387564898464 38.0 33.0 39.0 23.0 40.0 22 33.554887826873916 37.0 32.0 39.0 23.0 40.0 23 33.94512011177619 38.0 33.0 39.0 23.0 40.0 24 33.67371354174936 38.0 32.0 39.0 23.0 40.0 25 33.806773256275505 38.0 33.0 39.0 23.0 40.0 26 34.55957163043996 38.0 34.0 39.0 26.0 40.0 27 34.39811536446343 38.0 33.0 39.0 25.0 40.0 28 34.36923455535621 38.0 33.0 39.0 25.0 40.0 29 34.209858215709 38.0 33.0 39.0 25.0 40.0 30 34.035295238397666 38.0 33.0 39.0 24.0 40.0 31 34.26540495054221 38.0 33.0 39.0 26.0 40.0 32 33.98003429496848 38.0 33.0 39.0 24.0 40.0 33 34.131471984503754 38.0 33.0 39.0 25.0 40.0 34 33.86379658002953 38.0 33.0 39.0 24.0 40.0 35 33.804161440388675 38.0 33.0 39.0 23.0 40.0 36 33.005382404775894 36.0 32.0 38.0 23.0 39.0 37 32.76238826349967 36.0 32.0 38.0 22.0 39.0 38 32.52638013432196 35.0 31.0 38.0 21.0 39.0 39 32.19952209326326 35.0 31.0 38.0 18.0 39.0 40 28.785545305876187 35.0 22.0 38.0 5.0 39.0 41 25.65381928456885 31.0 13.0 37.0 4.0 39.0 42 29.56732769159932 33.0 27.0 38.0 4.0 39.0 43 28.10404394836702 33.0 23.0 38.0 4.0 39.0 44 28.056808980201005 33.0 23.0 38.0 4.0 39.0 45 29.095319371894004 33.0 27.0 38.0 4.0 39.0 46 28.56974104123335 34.0 25.0 38.0 4.0 39.0 47 28.138045186796436 34.0 23.0 38.0 4.0 39.0 48 28.208373688138067 33.0 24.0 38.0 4.0 39.0 49 25.758410999793597 32.0 10.0 38.0 4.0 39.0 50 27.842727402632455 33.0 23.0 38.0 4.0 39.0 51 28.052895225695824 34.0 23.0 38.0 4.0 39.0 52 27.76248352730102 34.0 21.0 38.0 4.0 39.0 53 27.415794738262704 34.0 18.0 38.0 4.0 39.0 54 26.522871250972486 33.0 10.0 38.0 4.0 39.0 55 26.803526348379723 33.0 4.0 38.0 4.0 39.0 56 25.757720337233856 33.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.211672494951614 1 2 2.3274008785059834 1 3 1.7395719115316908 1 4 2.7555114232505744 1 5 1.87557855816204 1 6 2.554220304128407 1 7 1.9242775974260837 1 8 2.2222704290682955 1 9 2.207588353613893 1 10 2.3331224899052074 1 11 -3.0530313869197414 1 12 -12.49622046460238 1 13 -7.75896678463306 1 14 -5.611244952735817 1 15 -5.2148273015572855 1 16 -22.711005950842303 1 17 -5.04810767878045 1 18 -12.803179405901183 1 19 -5.148032030242085 1 20 -4.933417931932791 1 21 -5.134542812990986 1 22 -2.357651773450165 1 23 -2.4188986769679346 1 24 -2.9296200136717836 1 25 -2.4342234651008177 1 26 2.71597807379338 1 27 2.8385944979420614 1 28 2.5788415610955226 1 29 2.6362047894640597 1 30 3.3089465253449433 1 31 3.044971868987588 1 32 3.2532410382645622 1 33 2.792804041578364 1 34 2.4535993082429712 1 35 2.345087776380673 1 36 3.1811688869380568 1 37 3.907668336160672 1 38 3.6165026945232377 1 39 4.036708102355107 1 40 6.204737650361071 1 41 -8.782605845736086 1 42 -8.752234993588978 1 43 -16.928884003705708 1 44 -20.77735846455537 1 45 -12.327319808538457 1 46 -17.137526706533798 1 47 -21.897170750550345 1 48 -15.418423453769286 1 49 -18.701433491552198 1 50 -14.814934534474979 1 51 -12.498658138357301 1 52 -11.823692244290799 1 53 -11.844707306475707 1 54 -11.268225054030655 1 55 -11.271252154885707 1 56 -7.247664944350863 2 1 1.7044400937944317 2 2 2.7787027107142777 2 3 2.229982283330685 2 4 3.199420134275968 2 5 2.1241588646015543 2 6 3.1747067500729003 2 7 2.7014124951010032 2 8 2.7997910548049347 2 9 2.562029583657825 2 10 2.603346426474374 2 11 1.3001240829184653 2 12 -2.282998563820211 2 13 -1.6989646416993835 2 14 3.2279278748651663 2 15 3.252010799803479 2 16 2.0035552834874935 2 17 2.8956403122192285 2 18 -3.2065866704463453 2 19 2.043974827145675 2 20 2.4331308733816854 2 21 2.817134032610639 2 22 2.567988427985604 2 23 2.3685223023527584 2 24 1.1554544532734639 2 25 2.4070922961763728 2 26 2.4285035211307857 2 27 2.710554210788949 2 28 2.7122391824391414 2 29 3.1854386906748218 2 30 2.964684230262975 2 31 2.00468471587498 2 32 2.9393012547008652 2 33 1.702157947074987 2 34 2.3512742252042926 2 35 2.272763764808829 2 36 3.188133421385711 2 37 2.6976608358928083 2 38 3.1653080089987533 2 39 3.0311364747969805 2 40 5.994408710041778 2 41 -5.977291370219103 2 42 -5.881668187631625 2 43 -12.774807072386732 2 44 -13.427203101863844 2 45 -6.402001047154123 2 46 -10.634794466096643 2 47 -17.560837310070326 2 48 -8.322955758494455 2 49 -12.169450206434872 2 50 -7.258093218713704 2 51 -2.6975223835089146 2 52 -2.4498574644772333 2 53 -2.609413188828647 2 54 -1.3677643232902703 2 55 -1.7061605444710501 2 56 0.7878006079902917 3 1 1.7315511646515347 3 2 3.076290489599046 3 3 2.3669867817154895 3 4 3.243649219805654 3 5 2.495049182507543 3 6 3.2819132229165433 3 7 2.546384266981569 3 8 2.8427521392477644 3 9 2.9612084020015743 3 10 2.9645455932165135 3 11 3.0043477341577898 3 12 5.2022231854174805 3 13 4.087709270687775 3 14 3.5349652808972323 3 15 3.7881337355279 3 16 5.320193269177196 3 17 5.337116023960313 3 18 5.455189134885298 3 19 4.884719928736715 3 20 5.015052800743334 3 21 4.34250914959884 3 22 3.6719947076100325 3 23 4.012546092468384 3 24 4.235006426222775 3 25 3.977360689739754 3 26 3.097374094448419 3 27 2.513349851280445 3 28 2.818902226245555 3 29 2.725758106186703 3 30 3.141359048365196 3 31 3.0250029237667775 3 32 2.7507313787838257 3 33 2.6405995133582323 3 34 2.714315012572584 3 35 2.5954398509121432 3 36 4.068035604159011 3 37 4.072294776055234 3 38 3.956480306194056 3 39 4.312481763569139 3 40 7.972007357507284 3 41 8.92612921366521 3 42 5.514944438036341 3 43 -0.6847789952198085 3 44 3.5560109789863183 3 45 5.758008613806538 3 46 3.6009631645527556 3 47 -0.7201223989813563 3 48 4.184003152232229 3 49 6.343848709726103 3 50 4.503160290932161 3 51 5.773721163271269 3 52 5.661303964137325 3 53 5.243257161079271 3 54 6.448526471625648 3 55 5.6796740719586225 3 56 6.0660966560535705 4 1 1.811581822844623 4 2 2.896141669620121 4 3 2.2819422315286673 4 4 3.2400404949948722 4 5 2.5441342270577323 4 6 3.140151015526648 4 7 2.5725076023374953 4 8 2.712390947410512 4 9 2.719583517768193 4 10 1.868579061743965 4 11 2.764415437667516 4 12 5.446051627278685 4 13 4.826699466453114 4 14 4.701158235899946 4 15 4.559474712119091 4 16 4.758222202846916 4 17 5.384284931442828 4 18 5.3709110395255415 4 19 4.465213653387821 4 20 4.454167545418073 4 21 3.76779577176854 4 22 4.366402780934209 4 23 3.7722305685645736 4 24 4.172763907141238 4 25 3.8584963203085323 4 26 2.863924728370428 4 27 3.0488782260768588 4 28 3.1835111746056626 4 29 2.8096529741329235 4 30 3.599890648659006 4 31 2.6485458612049015 4 32 2.906609129238909 4 33 2.8945834816780973 4 34 2.568337303633804 4 35 2.382716700782808 4 36 3.299760446876096 4 37 3.5187227772049177 4 38 3.8416717602227344 4 39 3.9306595172177765 4 40 6.987049033488507 4 41 9.557943476112111 4 42 5.95032271542912 4 43 7.586322464237924 4 44 6.883830095647134 4 45 6.3020957341718855 4 46 6.0378104803640795 4 47 4.894925664123427 4 48 4.872407329411036 4 49 7.400119268280061 4 50 5.527654909780562 4 51 3.958404840977014 4 52 4.611420529142276 4 53 4.336636907510467 4 54 5.174103707917283 4 55 3.7862112929211627 4 56 4.711834847858892 5 1 2.012516088202865 5 2 3.230729651875798 5 3 2.575261226017176 5 4 3.3536646380248527 5 5 2.6516083499237055 5 6 3.210893050746435 5 7 2.8593741166539317 5 8 2.6443710522633097 5 9 2.7968116398276095 5 10 2.5311009060778815 5 11 3.3120696922716277 5 12 5.473037781331648 5 13 4.926813729122827 5 14 4.96429844291702 5 15 5.448001395813137 5 16 5.969910601825276 5 17 5.343251190348596 5 18 6.32712155169116 5 19 5.369333830831025 5 20 5.583149935524268 5 21 4.323011526574298 5 22 4.341466633602579 5 23 4.508787901539442 5 24 5.049024157174848 5 25 4.193151515899338 5 26 3.395716635884881 5 27 3.697083630028999 5 28 3.5883034854001252 5 29 3.891182749640379 5 30 3.597706215267422 5 31 3.491468297016162 5 32 3.2025494437973165 5 33 3.2266845705341325 5 34 2.879803898606248 5 35 2.4601128561800323 5 36 3.9290789036579206 5 37 4.194452041891029 5 38 4.55258720584715 5 39 4.214736678126506 5 40 8.77173111441336 5 41 10.286401602204332 5 42 6.422563608022205 5 43 8.031140316099737 5 44 7.423317930033477 5 45 6.977589752185061 5 46 6.593093448504959 5 47 6.6974864921917145 5 48 6.3437744486474905 5 49 9.225835090299146 5 50 6.865906018800597 5 51 5.544471516605455 5 52 5.420265804044817 5 53 6.002989911946546 5 54 6.321872225191111 5 55 5.300225273294867 5 56 5.526426862914679 6 1 2.1802999459320063 6 2 3.229361662819713 6 3 2.5604869442114264 6 4 3.3202173056035136 6 5 2.5527027411685737 6 6 3.46075625184082 6 7 3.072643610497984 6 8 2.905930559787251 6 9 2.8481112294308915 6 10 2.5618806598398507 6 11 3.491413057524703 6 12 5.239727247815921 6 13 5.0423404049094245 6 14 5.199866158375293 6 15 5.1740615873316 6 16 5.712523460922405 6 17 5.33032369376857 6 18 5.908585299981173 6 19 4.856543133156606 6 20 5.480961153034528 6 21 4.047156533414238 6 22 3.9436554160923194 6 23 4.460839885123569 6 24 4.932471489596189 6 25 4.356626208101801 6 26 3.5015989888260606 6 27 3.4340193345433647 6 28 3.2474690120759107 6 29 3.821825704496746 6 30 3.693260250835138 6 31 3.312945725196741 6 32 4.069580907545607 6 33 3.2379020807940435 6 34 3.443141791903102 6 35 3.299989737164985 6 36 4.356985880402103 6 37 4.452112780605116 6 38 4.587744524588597 6 39 4.75174078209367 6 40 8.539378173236887 6 41 5.198439905897899 6 42 6.613124483535206 6 43 8.054122532242005 6 44 7.244863757666856 6 45 6.040654047670692 6 46 5.923257607191687 6 47 5.766227942260116 6 48 5.626674585446398 6 49 7.988762586879172 6 50 6.0131700406884185 6 51 5.325524868178647 6 52 5.642974422375872 6 53 5.50039073273998 6 54 6.681448148583723 6 55 5.964041962761353 6 56 5.843184728851753 7 1 1.358672612363847 7 2 2.5805416557749794 7 3 2.010715898068419 7 4 2.834412444737012 7 5 2.016668926445078 7 6 2.930604790058503 7 7 2.518892289610342 7 8 2.459717104345195 7 9 2.4423345475006357 7 10 2.064522435112835 7 11 2.57985969366419 7 12 4.750540914599995 7 13 4.355549281274342 7 14 4.518886940340778 7 15 5.116118649677503 7 16 5.012488237252093 7 17 4.3462800164173885 7 18 5.405696959016044 7 19 4.022411396629664 7 20 4.1674000399665445 7 21 3.1329613942807413 7 22 4.0685937746692815 7 23 3.3298782789242054 7 24 3.5757613804028097 7 25 3.0642697445582954 7 26 2.9226627436693136 7 27 2.4976685067871145 7 28 2.505341502389399 7 29 2.8523998503227403 7 30 2.933697024346934 7 31 2.641934805858945 7 32 2.9270307138154195 7 33 2.1384656595189497 7 34 2.4212326689724932 7 35 2.1903384515010202 7 36 2.7575846827973187 7 37 3.1435886523909566 7 38 3.0312809810873667 7 39 2.889747122354329 7 40 6.231311952736714 7 41 -0.6949380440844877 7 42 3.1320748181600706 7 43 4.82016691406659 7 44 4.666772880597463 7 45 3.4404779293191616 7 46 4.630936367318498 7 47 4.399373416018481 7 48 4.414029287806386 7 49 7.155440994769275 7 50 4.81553002659895 7 51 5.574098309531237 7 52 5.502009293809472 7 53 5.113141701390912 7 54 6.081272030232192 7 55 5.136285710559871 7 56 4.637936401976091 8 1 1.8036832122820243 8 2 2.9308596533202618 8 3 2.1664789062136194 8 4 3.1190481422860117 8 5 2.425044721438944 8 6 3.0718998397107526 8 7 2.8401627920836603 8 8 2.921129682914767 8 9 2.8276508713746225 8 10 2.9520889170557822 8 11 3.4250265029028526 8 12 5.518718844454575 8 13 5.16605249844249 8 14 4.968992939522536 8 15 5.347791949041508 8 16 5.795022547513561 8 17 5.8830821884707944 8 18 5.685311642692167 8 19 4.7639965493056735 8 20 5.00211570181056 8 21 3.835903336483298 8 22 4.1150474511672215 8 23 3.612319607447283 8 24 4.269665497634186 8 25 3.471715722168291 8 26 2.855343965077431 8 27 2.4271139636993766 8 28 2.553022537834039 8 29 3.19025829492422 8 30 2.304805738595455 8 31 2.765265783654904 8 32 3.0048750251926677 8 33 2.847730359931788 8 34 3.0085053286221424 8 35 2.8034972271174894 8 36 3.355122526364841 8 37 3.547947636286519 8 38 3.235022566240044 8 39 3.907897157687387 8 40 6.090054095779816 8 41 -1.0722579384572342 8 42 4.414330183206378 8 43 6.071440763882485 8 44 6.377636866910549 8 45 4.8376215303159285 8 46 4.982556109201191 8 47 5.5749601307877015 8 48 5.138877727572815 8 49 7.457743222611729 8 50 5.373308878832372 8 51 5.564874521083283 8 52 5.893759953979817 8 53 6.13385803270344 8 54 6.366614388992932 8 55 6.28263896678035 8 56 6.148592109195192 9 1 1.8171532414670892 9 2 2.9554424565830004 9 3 2.547942649385064 9 4 2.9557240384171166 9 5 2.516416419410959 9 6 3.2978221625424524 9 7 2.9423105134037257 9 8 3.008198454897105 9 9 3.0267079693316674 9 10 2.7340989447441757 9 11 3.146196898772047 9 12 5.388770479566453 9 13 5.287769178828626 9 14 4.953090821734612 9 15 5.208788731201203 9 16 5.715287458087531 9 17 5.454136592422003 9 18 5.655714995232767 9 19 4.926422651228897 9 20 5.178835001369045 9 21 3.770685945178947 9 22 4.119275543661416 9 23 4.126537971587069 9 24 4.443952708589812 9 25 4.424308702786419 9 26 3.112584105764398 9 27 3.3948981438984305 9 28 3.4507999830185057 9 29 3.2443629121508906 9 30 3.5659746377947386 9 31 3.2682965402215416 9 32 3.2518629769998952 9 33 2.9780154755495403 9 34 2.2852395948797124 9 35 2.9199755628205537 9 36 4.195540097269575 9 37 4.160759130711423 9 38 4.558191016438347 9 39 5.093484226459367 9 40 7.160285331280829 9 41 4.431820487046021 9 42 5.971877141224784 9 43 7.5776519440067105 9 44 7.09794667758181 9 45 6.153269214219243 9 46 6.733959237241301 9 47 7.402656007462099 9 48 7.026178483391117 9 49 9.22639547135827 9 50 7.294247290865595 9 51 6.745298078667659 9 52 6.984009149519736 9 53 7.007052674625168 9 54 6.956713917541908 9 55 5.586650042137681 9 56 5.156225125733396 10 1 1.9814271033818116 10 2 3.035631476134263 10 3 2.453829670523511 10 4 3.229622025575331 10 5 2.4118785072834044 10 6 3.3377904188010206 10 7 2.488952169849867 10 8 2.549537463626635 10 9 2.7297596972012315 10 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1.502704395162418 115 34 2.5616567388654943 115 35 2.2181189560656662 115 36 3.6225596508939084 115 37 3.5637810544720416 115 38 3.4494610144632354 115 39 4.052994398294253 115 40 7.988944229167455 115 41 6.558227755463957 115 42 1.4462006648273942 115 43 -5.586282482222799 115 44 -7.36709381224636 115 45 1.1417147998404147 115 46 -5.544630531670414 115 47 -4.67832364886814 115 48 -5.309294556308664 115 49 -14.65578900424908 115 50 -4.774486661336653 115 51 -3.0089977933449816 115 52 -3.165704459012751 115 53 -3.7432929702494135 115 54 -7.406015689410491 115 55 -2.730750708405477 115 56 -1.6848769876381233 116 1 1.6723167526546945 116 2 2.6541515787860988 116 3 1.931075179505548 116 4 2.8895450366959494 116 5 2.2157279512526102 116 6 3.101092386294603 116 7 2.4243242827668894 116 8 2.476098627014146 116 9 2.3720608092628197 116 10 2.350536122024721 116 11 -2.4535449256685737 116 12 -12.81699544125971 116 13 -9.021525141309066 116 14 -3.7589573710364697 116 15 -5.92425774039949 116 16 -4.080753850001965 116 17 -2.079340171777652 116 18 -8.63301133867429 116 19 -2.700599723986251 116 20 -3.3665178385621104 116 21 2.577828802321804 116 22 3.05542012197467 116 23 2.620083582602561 116 24 2.784572329932324 116 25 3.326374107261465 116 26 2.8171452073134517 116 27 2.750405889165215 116 28 2.294948003672552 116 29 2.7008173011353946 116 30 3.2327560491544673 116 31 3.001601187381617 116 32 3.440589310906951 116 33 2.9803390555839613 116 34 2.65322582551655 116 35 2.9205779724591068 116 36 4.17841445183068 116 37 4.557574965479066 116 38 4.621357969966748 116 39 5.283673555202917 116 40 8.328873971556217 116 41 6.063145788250843 116 42 0.5177463322747009 116 43 -7.443776627421862 116 44 -7.622363133089451 116 45 -0.554303935522583 116 46 -4.35657432558142 116 47 -2.909822477907955 116 48 -4.174165750688054 116 49 -9.373002119003178 116 50 -3.7158215559502352 116 51 -2.559349081401187 116 52 -1.9956810398090035 116 53 -2.876079855495316 116 54 -10.442081263180981 116 55 -3.424193331356296 116 56 -3.0622774560221977 117 1 0.43358048891843026 117 2 1.4527779524124753 117 3 1.1003059487363132 117 4 1.8313032784541932 117 5 1.2797389402635986 117 6 1.8475209577231766 117 7 1.414159447602053 117 8 1.4791206050361225 117 9 1.455577292779303 117 10 1.5975141440027443 117 11 -3.4128855850092314 117 12 -11.128259177523447 117 13 -10.65256909735302 117 14 -10.32818814026724 117 15 -10.747884114025862 117 16 -14.219490113738232 117 17 -13.817526984964463 117 18 -15.068450899113852 117 19 -6.3799953283818525 117 20 -6.701957399001671 117 21 -0.04662174712874645 117 22 0.030420121974671588 117 23 0.18052314304212302 117 24 -0.3132298678698717 117 25 -0.38488962900227364 117 26 2.5687935589618007 117 27 2.172933361692685 117 28 2.1842337179582643 117 29 2.1326854330035303 117 30 2.081382422780841 117 31 1.5024253632057878 117 32 1.4760288713465144 117 33 1.013580813825719 117 34 1.1306983529890786 117 35 0.8301933570744922 117 36 1.8166012650174928 117 37 1.8413661742702736 117 38 1.4007535743623514 117 39 2.6847724563018147 117 40 5.52173111441336 117 41 2.926607326712382 117 42 -1.967418502890137 117 43 -8.268501902147136 117 44 -8.542692803419119 117 45 -2.0847984410170888 117 46 -3.6727831167902103 117 47 -3.9243829174683924 117 48 -4.010429486951789 117 49 -8.026848272849332 117 50 -3.4303819955106754 117 51 -3.6719864440385486 117 52 -3.481120600248566 117 53 -4.718387547803008 117 54 -9.569004340104058 117 55 -4.297270254433219 117 56 -3.811453280198023 118 1 0.9206129518683284 118 2 1.8882931253129698 118 3 1.5283228859275653 118 4 2.403109912187432 118 5 1.6549153693391077 118 6 2.5688512329525324 118 7 1.7544029197262603 118 8 2.081537683370634 118 9 2.087722670337527 118 10 2.217574129888419 118 11 -2.5238594735060573 118 12 -6.929511823959579 118 13 -10.392953712737636 118 14 -10.88923260039427 118 15 -6.1111515805043375 118 16 -11.386127375558978 118 17 -11.80561802236743 118 18 -11.913810814427894 118 19 -5.0801541145498135 118 20 -5.703280617068007 118 21 0.5275666791239004 118 22 1.5911999384884297 118 23 1.313727095053416 118 24 0.4835238371407087 118 25 1.5546834126349864 118 26 2.781037736802311 118 27 2.566723058234679 118 28 2.247307112453676 118 29 2.547999476757937 118 30 3.131576494763898 118 31 1.564969470474665 118 32 -3.123565341779809 118 33 2.075948492019087 118 34 2.1612205830525895 118 35 1.8320458623673588 118 36 3.3046923024204524 118 37 3.687784664037391 118 38 3.692655479796368 118 39 3.821681418898855 118 40 8.076776985972995 118 41 4.657641201094879 118 42 -1.2292392509494157 118 43 -7.9406966798465 118 44 -8.33009576742759 118 45 -1.68104050170869 118 46 -10.168107746289152 118 47 -11.650123919585543 118 48 -10.312828922378749 118 49 -12.338598449278408 118 50 -8.761627584783788 118 51 -7.01020098179437 118 52 -6.7484988641864625 118 53 -5.909107378431095 118 54 -12.187035391621349 118 55 -5.81685388181501 118 56 -3.434688989442904 119 1 0.5052741461384045 119 2 1.5487631076081527 119 3 1.0591453549441425 119 4 1.731775613137053 119 5 1.356830708144841 119 6 1.7005911331617725 119 7 1.2577829293834313 119 8 1.4469006320266757 119 9 1.471434242846783 119 10 1.3446463977139445 119 11 -5.861940915643512 119 12 -10.660479150532893 119 13 -13.006988800456934 119 14 -11.830887195597874 119 15 -10.144307865712772 119 16 -13.845164877570888 119 17 -16.136858968770134 119 18 -16.13238477225825 119 19 -6.95573756859778 119 20 -9.371154430040807 119 21 -1.229314054821053 119 22 0.3396306482904592 119 23 -0.1524728083748812 119 24 -0.18434997583208457 119 25 0.4381535558830123 119 26 1.6150148814989151 119 27 1.8119347112203528 119 28 1.8223916126951067 119 29 1.8391631657970535 119 30 2.361578104292306 119 31 -0.32534116850811046 119 32 -4.758282869544175 119 33 0.17670497037092758 119 34 -0.2933907158368356 119 35 0.42145516544156436 119 36 2.083301669875791 119 37 2.3459208301407237 119 38 1.6607063408940697 119 39 1.4465808233733384 119 40 5.442783745992305 119 41 0.8070054373736504 119 42 -4.047040635143848 119 43 -10.694277880554694 119 44 -8.364217769680927 119 45 -6.339015714971204 119 46 -16.365596881972397 119 47 -14.1543086934468 119 48 -14.632561740662991 119 49 -12.842300364617213 119 50 -12.0199568942961 119 51 -10.608221261852314 119 52 -9.878387806726298 119 53 -10.355027223916368 119 54 -12.224537403531858 119 55 -9.668052980479102 119 56 -7.590974197876832 120 1 0.12588818122612366 120 2 0.959508721643239 120 3 0.5060751795055438 120 4 1.096687893838805 120 5 0.7585850941097547 120 6 1.322520957723178 120 7 0.9939671399097492 120 8 1.0993129127284291 120 9 1.1238465235485364 120 10 1.014821836310439 120 11 -3.8696163542400015 120 12 -12.033066869831138 120 13 -11.342953712737636 120 14 -12.00895737103647 120 15 -10.704614883256632 120 16 -15.702182421430539 120 17 -14.42041160034908 120 18 -15.597297052960005 120 19 -7.430956866843392 120 20 -13.055803552847824 120 21 -6.004314054821052 120 22 -4.057079878025327 120 23 -3.3531307031117237 120 24 -9.631499098639104 120 25 -2.702197321309967 120 26 1.520716635884881 120 27 0.9200487463080691 120 28 1.4842337179582614 120 29 1.4240315868496864 120 30 1.8006131920116104 120 31 -0.3966130983326721 120 32 -11.570124974807332 120 33 0.24819619844110719 120 34 -0.32891703162631103 120 35 -0.2919220275408918 120 36 1.8944858804021045 120 37 2.168289251193354 120 38 2.5767151128238943 120 39 2.3318878409172044 120 40 6.2842311144133625 120 41 -0.8493542117491586 120 42 -5.112610810582442 120 43 -9.79734805599329 120 44 -9.658077418803735 120 45 -9.59537536409401 120 46 -15.245860039867134 120 47 -15.38303676362224 120 48 -12.052737179259482 120 49 -16.93550211900318 120 50 -11.464035841664522 120 51 -9.74506336711547 120 52 -10.209966754094719 120 53 -9.663579855495314 120 54 -12.192081263180981 120 55 -9.774193331356296 120 56 -7.035491741736482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1986.0 5 307.0 6 200.0 7 108.0 8 333.0 9 370.0 10 294.0 11 248.0 12 254.0 13 267.0 14 340.0 15 391.0 16 451.0 17 597.0 18 757.0 19 912.0 20 1054.0 21 1391.0 22 1641.0 23 2039.0 24 2281.0 25 2542.0 26 2760.0 27 3087.0 28 3679.0 29 4367.0 30 4988.0 31 5728.0 32 6397.0 33 7558.0 34 8915.0 35 10679.0 36 13484.0 37 15927.0 38 13167.0 39 6460.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.96956321547084 0.8946858676150706 0.07859263610815617 0.05715828080593176 8 98.70123684168743 1.1090294206373148 0.09208834129844562 0.09764539637680009 9 95.50672403664481 2.807900544591398 0.9939189940142578 0.6914564247495356 10 46.42840131464046 36.51382118984488 10.074940857056665 6.9828366384579965 11 37.72446533191496 29.631011542797264 17.194322277439944 15.450200847847832 12 38.88588984329105 14.66427448676627 20.167346744359588 26.282488925583092 13 26.08992528721943 15.585673566284767 24.378121303067115 33.94627984342869 14 23.139334566499688 19.860593983947666 29.330835245270436 27.66923620428221 15 28.85077405291881 17.925729105344384 27.815786542775772 25.407710298961035 16 34.168447307591485 20.110254108713093 23.883359414096322 21.837939169599096 17 30.359779623072892 22.47431846688789 24.39070860390899 22.775193306130227 18 27.487000357242092 24.1392450283809 24.016194974794587 24.357559639582423 19 28.194004115913007 27.39306969241893 21.743621526701787 22.66930466496627 20 28.956421562785522 26.944742382711635 22.186469630159298 21.912366424343542 21 27.452645952082307 23.62304113808488 26.398393217217343 22.525919692615467 22 24.92577362145341 23.835003096073542 27.720972325865713 23.51825095660734 23 27.855929377768607 22.03134179064192 27.304193194989125 22.808535636600354 24 28.76672091453975 22.395109752709086 26.796332314531814 22.041837018219347 25 27.451494077672983 21.86180178463688 26.732240957733953 23.95446317995618 26 22.87007264499226 20.223889484339644 31.966178397046562 24.93985947362153 27 21.5237550358769 18.473225424523427 33.393724125326784 26.60929541427289 28 21.09931332655137 22.266817141403866 32.665469226856565 23.9684003051882 29 25.351016518424395 19.876111817026683 34.45918043202033 20.313691232528587 30 23.301757199914245 17.52515106519823 38.712392507483784 20.46069922740374 31 24.064765625620378 17.812929302554572 38.77599637896944 19.34630869285561 32 26.70332202420207 16.70944007881961 39.006968225843615 17.5802696711347 33 24.877152807595056 15.975700904855048 41.70682060334271 17.440325684207178 34 19.896265230583488 17.701631479451617 47.5361006529095 14.866002637055395 35 18.063332459672615 20.859046756733143 47.07125100272423 14.006369780870006 36 18.543519695044473 23.271124523506987 42.92487293519695 15.260482846251588 37 21.03643261040727 20.468589697707156 43.059034985167685 15.435942706717883 38 21.542502387774594 20.803884113339702 42.156160458452725 15.497453040432982 39 21.446576388171028 19.311540327133645 41.63280560437027 17.609077680325065 40 16.41393966509012 13.346861817716988 38.904512335421195 31.334686181771698 41 12.413864856861599 14.736412456282164 35.913852851963604 36.935869834892635 42 17.233677249097816 19.06029919081761 43.42877128276871 20.277252277315867 43 14.557359832602693 14.640899362712728 43.77391816308507 27.027822641599503 44 15.997990382545039 15.447737984162298 42.279320876894985 26.27495075639768 45 20.168474975341244 17.717712940278087 43.71519933819084 18.398612746189823 46 17.855893112772385 20.081915062828056 42.82010497852712 19.242086845872436 47 16.03929416622989 18.94109051635139 44.46800400247773 20.551611314940995 48 18.03566743259682 18.587455177184292 45.952628942618844 17.42424844760004 49 16.266194396579234 15.324845235815943 39.23511391920352 29.173846448401303 50 18.543835943088784 18.41745489229791 43.45679993641205 19.581909228201255 51 16.711918657886983 20.390467621603115 44.635449427913976 18.262164292595926 52 16.312597855717954 18.655571715823022 45.126089824594864 19.905740603864157 53 16.147344819635375 18.45388119866744 46.508392103233604 18.89038187846358 54 14.993913645368403 19.080125068620664 43.061157919023636 22.864803366987292 55 17.375113388608625 19.859318554354918 42.32856438085841 20.437003676178048 56 18.7669500624319 21.008931340814556 39.72736744156454 20.496751155189006 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 8.0 7 16.0 8 45.0 9 74.0 10 181.0 11 288.0 12 405.0 13 619.5 14 717.0 15 904.5 16 1092.0 17 1352.5 18 1613.0 19 1717.5 20 1822.0 21 2113.5 22 2807.0 23 3209.0 24 3725.5 25 4242.0 26 4732.5 27 5223.0 28 5675.5 29 5921.0 30 5714.0 31 5186.0 32 4658.0 33 3960.5 34 3263.0 35 2835.5 36 2408.0 37 2254.5 38 2087.5 39 2074.0 40 2048.0 41 2022.0 42 2145.0 43 2268.0 44 2607.0 45 2946.0 46 3074.5 47 3284.5 48 3366.0 49 3508.5 50 3651.0 51 3902.0 52 4153.0 53 4509.0 54 5338.0 55 5811.0 56 6229.0 57 6647.0 58 7346.5 59 8046.0 60 8250.0 61 8454.0 62 7634.0 63 5929.5 64 5045.0 65 4332.5 66 3620.0 67 3073.0 68 2526.0 69 2155.5 70 1785.0 71 1532.5 72 1072.5 73 865.0 74 700.0 75 535.0 76 391.5 77 248.0 78 197.0 79 104.5 80 63.0 81 45.0 82 27.0 83 17.5 84 8.0 85 5.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.013495705190289442 14 0.00238159503358049 15 0.2095803629550831 16 0.059539875839512243 17 0.0 18 7.938650111934966E-4 19 0.08970674626486512 20 0.08018036613054316 21 0.0 22 0.0 23 0.0 24 7.938650111934966E-4 25 0.0015877300223869932 26 0.008732515123128464 27 0.0944699363320261 28 0.11272883158947651 29 0.036517790514900846 30 0.02143435530222441 31 0.02778527539177238 32 0.08653128622009114 33 0.15877300223869933 34 0.05398282076115777 35 0.046838035660416305 36 0.036517790514900846 37 0.18020735754092373 38 0.2587999936490799 39 0.1643300573170538 40 0.6335042789324103 41 0.8073607163837861 42 0.12701840179095947 43 0.21990060810059855 44 0.4517091913690996 45 0.19846625279837415 46 0.17941349252973024 47 0.034930060492513855 48 0.15321594716034484 49 0.487433116872807 50 0.12384294174618549 51 0.29611164917517424 52 0.11352269660067002 53 0.15321594716034484 54 0.2183128780782116 55 0.231808583268501 56 0.18179508756331073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 125966.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.70585713605259 #Duplication Level Percentage of deduplicated Percentage of total 1 85.7359535012455 61.47770033183557 2 8.148353169111541 11.685692964768272 3 2.7113202324937724 5.83252623723862 4 1.1668973152504842 3.346934887191782 5 0.6310545253252144 2.2625152819014653 6 0.40409631884860225 1.7385643745137576 7 0.25574314973706064 1.283679723099884 8 0.17824522557431496 1.0224981344172237 9 0.1583171879324661 1.02170426940603 >10 0.5790202048159425 7.421050124636806 >50 0.023249377248823692 1.2138196021148564 >100 0.0066426792139496265 1.2622453677976597 >500 0.001107113202324938 0.43106870107806866 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 543 0.43106870107806866 No Hit ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 408 0.32389692456694663 No Hit ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 398 0.3159582744550117 No Hit ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 397 0.3151644094438182 No Hit ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 161 0.12781226680215296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.003969325055967483 0.0 0.0 0.0 0.0 6 0.003969325055967483 0.0 0.0 0.0 0.0 7 0.003969325055967483 0.0 0.0 0.0 0.0 8 0.003969325055967483 0.0 0.0 0.0 0.0 9 0.003969325055967483 0.0 0.0 0.0 0.0 10 0.0055570550783544765 0.0 0.0 0.0 0.0 11 0.00714478510074147 0.0 0.0 0.0 0.0 12 0.011114110156708953 0.0 0.0 0.0 0.0 13 0.011114110156708953 0.0 0.0 0.0 0.0 14 0.01428957020148294 0.0 0.0 0.0 0.0 15 0.01667116523506343 0.0 0.0 0.0 0.0 16 0.018258895257450423 0.0 0.0 0.0 0.0 17 0.019846625279837417 0.0 0.0 0.0 0.0 18 0.0238159503358049 0.0 0.0 0.0 0.0 19 0.025403680358191894 0.0 0.0 0.0 0.0 20 0.030166870425352873 0.0 0.0 0.0 0.0 21 0.03572392550370735 0.0 0.0 0.0 0.0 22 0.041280980582061824 0.0 0.0 0.0 0.0 23 0.04366257561564232 0.0 0.0 0.0 0.0 24 0.05080736071638379 0.0 0.0 0.0 0.0 25 0.05557055078354477 0.0 0.0 0.0 0.0 26 0.05636441579473826 0.0 0.0 0.0 0.0 27 0.05953987583951225 0.0 0.0 0.0 0.0 28 0.061921470873092736 0.0 0.0 0.0 0.0 29 0.06668466094025371 0.0 0.0 0.0 0.0 30 0.06747852595144721 0.0 0.0 0.0 0.0 31 0.06986012098502771 0.0 0.0 0.0 0.0 32 0.0706539859962212 0.0 0.0 0.0 0.0 33 0.0706539859962212 0.0 0.0 0.0 0.0 34 0.0714478510074147 0.0 0.0 0.0 0.0 35 0.0722417160186082 0.0 0.0 0.0 0.0 36 0.07382944604099519 0.0 0.0 0.0 0.0 37 0.07621104107457567 0.0 0.0 0.0 0.0 38 0.07859263610815617 0.0 0.0 0.0 0.0 39 0.08176809615293015 0.0 0.0 0.0 0.0 40 0.08256196116412365 0.0 0.0 0.0 0.0 41 0.08414969118651064 0.0 0.0 0.0 0.0 42 0.08653128622009114 0.0 0.0 0.0 0.0 43 0.08811901624247813 0.0 0.0 0.0 0.0 44 0.08970674626486512 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCACGG 15 0.008132153 50.432022 37 GTAAGCG 15 0.008171034 50.371235 35 GGTAAGC 15 0.008262293 50.22997 34 TTGTGAC 15 0.0082753915 50.209854 47 TGCAATA 15 0.0082753915 50.209854 47 CGATTAA 30 1.0135009E-6 50.18975 49 AATGCAA 15 0.008301635 50.16967 45 GCCCCGT 15 0.008447085 49.949802 11 AGTCCAA 15 0.008447085 49.949802 11 GTCTACG 30 1.0475942E-6 49.949802 10 TACGCTT 15 0.008447085 49.949802 12 GTCGTTC 15 0.008447085 49.949802 11 CATACAC 15 0.008447085 49.949802 12 GAGGCAT 15 0.008447085 49.949802 10 AGCTTAT 20 4.1902656E-4 49.9498 11 TTAAGCC 20 4.1902656E-4 49.9498 13 GAGAGTA 25 2.0940684E-5 49.92991 9 GAAGTTT 15 0.008460401 49.92991 9 GATTACG 15 0.008460401 49.92991 9 GACAGTA 20 4.1985148E-4 49.929905 9 >>END_MODULE