FastQCFastQC Report
Fri 17 Jun 2016
SRR1526254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58915
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2440.4141559874395315No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC910.15445981498769412No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.1289994059237885No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC720.12220996350674701No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG690.11711788169396589No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC640.108631078672664No Hit
TAAGGGGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG620.10523635746414325No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG590.10014427565136212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCAAA150.00825591350.20615836
GCAAGAA150.008311951550.1204146
CAACTCT150.008311951550.1204148
ACCCGCT150.008311951550.1204134
GAATGGC150.008311951550.1204144
AACTCTA150.008311951550.1204149
CACCTGC150.008311951550.1204143
CTTGTGC150.008311951550.1204149
CAGGCAC150.008311951550.1204149
GCATATT150.008311951550.1204149
AAGCATA150.008311951550.1204147
CCAAAAC402.5538611E-950.12040743
AATTCGT204.1195587E-450.07764413
GAAGTTA150.008340077550.0776449
TGTTCAC150.008340077550.07764411
GATGAGC150.008340077550.07764413
GAATTCG204.1195587E-450.07764412
AGATGGA252.0442378E-550.07764413
GGAATTC301.0198164E-650.07764411
TGTACGC150.008340077550.07764410