##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526254_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58915 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.07672069931257 39.0 38.0 39.0 35.0 40.0 2 37.022948315369604 39.0 38.0 39.0 33.0 40.0 3 36.69349062208266 39.0 38.0 39.0 32.0 40.0 4 37.37044895187983 39.0 38.0 39.0 33.0 40.0 5 37.51024357124671 39.0 38.0 39.0 34.0 40.0 6 37.51774590511754 39.0 38.0 39.0 35.0 40.0 7 37.84898582703895 39.0 38.0 39.0 35.0 40.0 8 37.722192989900705 39.0 38.0 39.0 35.0 40.0 9 36.920241025205804 39.0 35.0 39.0 32.0 40.0 10 36.23703640838496 39.0 35.0 39.0 31.0 40.0 11 35.301400322498516 38.0 34.0 39.0 28.0 40.0 12 33.94308749893915 38.0 32.0 39.0 22.0 40.0 13 33.852007128914536 38.0 33.0 39.0 22.0 40.0 14 33.97255367902911 38.0 33.0 39.0 22.0 40.0 15 33.9578884834083 38.0 33.0 39.0 22.0 40.0 16 33.41590426886192 37.0 31.0 39.0 21.0 40.0 17 33.30472714928287 36.0 31.0 39.0 21.0 40.0 18 32.810184163625564 36.0 30.0 39.0 19.0 40.0 19 33.64593057795128 36.0 31.0 39.0 23.0 40.0 20 33.655232114062635 36.0 31.0 39.0 23.0 40.0 21 34.42204871424934 38.0 33.0 39.0 25.0 40.0 22 34.042603751166936 37.0 33.0 39.0 23.0 40.0 23 34.534498854281594 38.0 33.0 39.0 26.0 40.0 24 34.1483662904184 38.0 33.0 39.0 24.0 40.0 25 34.38508020028855 38.0 33.0 39.0 25.0 40.0 26 34.95393363320037 38.0 34.0 39.0 27.0 40.0 27 34.84260375116693 38.0 34.0 39.0 27.0 40.0 28 34.77708563184248 38.0 34.0 39.0 27.0 40.0 29 34.56511923958245 38.0 33.0 39.0 26.0 40.0 30 34.36825935670033 38.0 33.0 39.0 25.0 40.0 31 34.57959772553679 38.0 34.0 39.0 26.0 40.0 32 34.33032334719511 38.0 33.0 39.0 26.0 40.0 33 34.40673852159891 38.0 33.0 39.0 26.0 40.0 34 34.20668760078079 38.0 33.0 39.0 25.0 40.0 35 34.14449630824068 38.0 33.0 39.0 25.0 40.0 36 33.4959348213528 36.0 32.0 38.0 25.0 39.0 37 33.1550199439871 36.0 32.0 38.0 23.0 39.0 38 32.82047016888738 36.0 32.0 38.0 23.0 39.0 39 32.38336586607825 35.0 31.0 38.0 19.0 39.0 40 28.983501654926588 35.0 23.0 38.0 7.0 39.0 41 25.865959433081557 31.0 14.0 37.0 4.0 39.0 42 29.81305270304676 34.0 28.0 38.0 4.0 39.0 43 28.41245862683527 33.0 24.0 38.0 4.0 39.0 44 28.388186370194347 33.0 24.0 38.0 4.0 39.0 45 29.366171603157092 33.0 27.0 38.0 4.0 39.0 46 28.99509462785369 34.0 26.0 38.0 4.0 39.0 47 28.55208351014173 34.0 24.0 38.0 4.0 39.0 48 28.60821522532462 34.0 25.0 38.0 4.0 39.0 49 26.23272511245014 32.0 13.0 38.0 4.0 39.0 50 28.208317066960877 33.0 24.0 38.0 4.0 39.0 51 28.51421539506068 35.0 25.0 38.0 4.0 40.0 52 28.17986930323347 34.0 23.0 38.0 4.0 40.0 53 27.811983365866077 34.0 22.0 38.0 4.0 39.0 54 26.90072137825681 33.0 15.0 38.0 4.0 39.0 55 27.140185012305864 33.0 17.0 38.0 4.0 39.0 56 26.038581006534837 33.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.040496622915192404 1 2 0.938296041484584 1 3 1.5861042663693823 1 4 0.5344754421590707 1 5 0.5169924731219737 1 6 0.4212318701655988 1 7 -0.0050670531485863535 1 8 0.14178825645647208 1 9 0.21932278035274777 1 10 -0.2002748257135707 1 11 -4.421771528819594 1 12 -12.81420955208836 1 13 -8.21324917372554 1 14 -6.935819345147056 1 15 -7.907986643277649 1 16 -22.938190714418948 1 17 -5.050029758253547 1 18 -11.82488190001888 1 19 -5.761698731570647 1 20 -6.084056369138942 1 21 -5.795549598357905 1 22 -3.1128067762221825 1 23 -3.709889179164268 1 24 -4.689466598674056 1 25 -2.8468944276170802 1 26 0.9664464679606155 1 27 0.8141987262949826 1 28 0.9313174217550824 1 29 1.2062130731873992 1 30 1.172479906609368 1 31 0.6079651063168043 1 32 0.4603616334772056 1 33 0.8314966447969425 1 34 0.027375441359403396 1 35 0.514991223384996 1 36 0.8809860928023383 1 37 1.595209236092586 1 38 0.9526162070410109 1 39 1.7651898972343112 1 40 3.116608686411734 1 41 -9.920071841163294 1 42 -9.62447336841182 1 43 -17.74596627289361 1 44 -21.364022443191974 1 45 -12.197524726846375 1 46 -16.84024865508357 1 47 -22.314068951878422 1 48 -14.536591305736124 1 49 -19.08206915012809 1 50 -14.593199724818936 1 51 -11.868165818947311 1 52 -11.761972497519167 1 53 -11.64895038168404 1 54 -11.25087318315534 1 55 -11.488180360403998 1 56 -7.669039397183582 2 1 0.5323947342524633 2 2 1.9721384573124183 2 3 2.329596109479411 2 4 0.9773772123777462 2 5 1.2689883079015658 2 6 0.9639253793637934 2 7 0.921173835431766 2 8 0.7540756566647318 2 9 1.0695483964582664 2 10 0.9271114984794195 2 11 0.5554933097781145 2 12 -3.313688899537162 2 13 -2.857469930407248 2 14 2.2738300821351345 2 15 2.3330754879267985 2 16 1.5564292092186776 2 17 2.141570380587133 2 18 -3.2863536112302647 2 19 1.6039729102203992 2 20 2.221219576713196 2 21 1.172690596019045 2 22 1.6367593466518215 2 23 1.2323183876528105 2 24 0.07242163457828354 2 25 0.7077740902586598 2 26 1.4820660444965412 2 27 2.266298691584815 2 28 0.9516228712517858 2 29 2.397813212028076 2 30 1.958144606366396 2 31 1.3037304655670638 2 32 1.86126409789928 2 33 1.6169877937035722 2 34 0.9890207034211898 2 35 1.5969072247734033 2 36 1.7563765822157364 2 37 2.1969100344264945 2 38 2.7537269324835663 2 39 2.45366612076781 2 40 4.701127950556128 2 41 -8.86644462838995 2 42 -8.590804503434384 2 43 -14.316254367305271 2 44 -15.101266455687634 2 45 -7.635393522403472 2 46 -11.042435395798599 2 47 -17.51937960790064 2 48 -9.576160899627133 2 49 -12.925699833918438 2 50 -9.30128719444754 2 51 -5.4785441598011815 2 52 -5.6616601059884495 2 53 -4.3863679450300985 2 54 -4.015885331714866 2 55 -4.86461215491979 2 56 -1.4383903170030905 3 1 0.9584244344485953 3 2 2.071185809511917 3 3 2.485942424973949 3 4 1.6064049512401724 3 5 1.6106806587001046 3 6 1.0570887304425227 3 7 1.169002358827676 3 8 1.3520022467459825 3 9 1.8533814029026487 3 10 1.5348447570952786 3 11 2.8913016594558982 3 12 3.9085021904040005 3 13 2.4148752643251683 3 14 3.7588398888036707 3 15 3.358152540322422 3 16 3.794591159344762 3 17 4.654879430741239 3 18 4.884702707066175 3 19 3.7184307415457027 3 20 3.404884468839839 3 21 3.1913232662347895 3 22 3.479712554833384 3 23 3.225733910208149 3 24 3.689961561728751 3 25 2.314946967815395 3 26 1.7803288632132706 3 27 2.0476099832631576 3 28 1.9002079090775084 3 29 1.7544957561580858 3 30 2.17823426733586 3 31 1.4801104039251527 3 32 1.839969617652713 3 33 1.6646201837287862 3 34 2.345843342815975 3 35 1.6593897128653055 3 36 3.2573572490691944 3 37 3.3713286390776602 3 38 3.081549852068889 3 39 3.4952744144074863 3 40 6.404686370281539 3 41 7.242129201814585 3 42 4.155062686254606 3 43 -2.239762352735365 3 44 4.143620067147893 3 45 3.4958757406954355 3 46 2.0086993758461205 3 47 -3.102596195179988 3 48 1.874665662435067 3 49 3.5667350217834404 3 50 1.6513606060568051 3 51 3.471161640142782 3 52 3.5367147889401025 3 53 3.2041335960178117 3 54 4.515355912331085 3 55 3.3044828296697304 3 56 3.9380456594609043 4 1 1.0193825526356264 4 2 2.0413521893168465 4 3 2.5397289998448613 4 4 1.467133580041093 4 5 1.2321666024752034 4 6 1.3387869518997846 4 7 0.8145846881449401 4 8 1.032868569178575 4 9 1.3477875991160744 4 10 1.517919914869232 4 11 3.0862242067880814 4 12 3.8961671359002708 4 13 3.952884443900615 4 14 3.771892097639011 4 15 3.9130865621239153 4 16 4.669662874777927 4 17 4.856686659656901 4 18 4.588231106377705 4 19 3.9254014816489686 4 20 4.139824227875984 4 21 3.0738533867167206 4 22 3.6602920155333223 4 23 2.98591750171704 4 24 3.725245554844065 4 25 3.159324477855556 4 26 1.349175679048038 4 27 1.5071624789602254 4 28 1.6241034914068209 4 29 1.0295961577645087 4 30 1.9761401766875508 4 31 1.1381712186124702 4 32 1.474220908530505 4 33 1.5729678601487507 4 34 1.759784823022521 4 35 1.320606006611719 4 36 2.229244799269999 4 37 2.0856143533633755 4 38 3.100626157289767 4 39 2.6953604729272804 4 40 6.450297385772075 4 41 9.082060354998752 4 42 5.666393724694078 4 43 6.4603237057844325 4 44 5.587825460148466 4 45 5.530442579479139 4 46 5.816645445840383 4 47 4.401993592542333 4 48 5.498589930937651 4 49 7.557412015472483 4 50 6.425456991598974 4 51 5.32959537508254 4 52 5.349967800988296 4 53 5.026422752644319 4 54 7.107868821109051 4 55 5.111855176198702 4 56 5.646881000826369 5 1 0.39597953289114685 5 2 1.211503176541008 5 3 2.0162098360817993 5 4 0.3832195266148446 5 5 0.4862316431256133 5 6 0.3173003084619168 5 7 0.4345033873319366 5 8 0.5203830871808961 5 9 0.8713067628791364 5 10 -0.2892810142829205 5 11 1.679429781700975 5 12 3.1894307828224555 5 13 2.5344756169552056 5 14 3.8308351870699084 5 15 3.6799274448184605 5 16 3.971346963047381 5 17 3.0944915377056788 5 18 4.298161419966561 5 19 3.3966558370496642 5 20 3.28616569129062 5 21 2.7384875330581835 5 22 2.7581433511895384 5 23 2.203106816467333 5 24 3.470890154147206 5 25 3.2060724453352307 5 26 1.3439491982118028 5 27 1.8573366949881063 5 28 1.7074368247401566 5 29 2.143417295975894 5 30 1.9706233358048522 5 31 1.9157554230259422 5 32 1.641468295290089 5 33 0.8438737834936987 5 34 1.6657081679702515 5 35 0.9332657046372717 5 36 2.615423661058614 5 37 2.4810847366386355 5 38 2.525422905257251 5 39 2.704942354460382 5 40 7.150645817827822 5 41 8.418297288796666 5 42 4.98491869565807 5 43 5.786978758049237 5 44 6.3837023474887715 5 45 5.484856052185297 5 46 5.632266816339804 5 47 5.929287436909348 5 48 5.894060314212911 5 49 8.320187857516618 5 50 5.785213089159949 5 51 5.616180740936201 5 52 5.4109434107443946 5 53 6.373983728254071 5 54 6.774245127729259 5 55 6.160345303957126 5 56 6.3460679926962875 6 1 0.803906362159438 6 2 2.140161713126375 6 3 2.2665147141305795 6 4 1.1576097705172828 6 5 1.077999935808542 6 6 1.0572393328521628 6 7 0.9229180214782744 6 8 0.9197733310833343 6 9 1.008501884830359 6 10 1.163158010107324 6 11 2.3522956353595106 6 12 4.384857612090748 6 13 3.5076463486625187 6 14 4.192725430972345 6 15 4.372610371647724 6 16 4.988115255730307 6 17 3.6941866596569 6 18 4.956088249234853 6 19 3.566472910220398 6 20 3.6648242278759824 6 21 2.611353386716722 6 22 2.785887253628566 6 23 2.506155596955132 6 24 2.0913169834154957 6 25 2.14265781118889 6 26 1.588461393333752 6 27 1.6750196218173699 6 28 2.0824368247401566 6 29 2.075429491097843 6 30 1.477330652878024 6 31 1.3447188376600892 6 32 1.1170780513876508 6 33 1.5533250030058952 6 34 0.8919276801653737 6 35 1.5890583875641013 6 36 1.4459114659366605 6 37 2.0338286390776616 6 38 1.8339594906231014 6 39 2.265003330070133 6 40 5.6610116714863565 6 41 2.7838460692844684 6 42 4.2068699151702695 6 43 5.304966562927284 6 44 5.406873079196089 6 45 4.036990198526762 6 46 3.7886692553641943 6 47 3.0579459734947108 6 48 3.9343042166519346 6 49 5.446102491662959 6 50 3.5004569915989734 6 51 2.379595375082541 6 52 2.249967800988294 6 53 2.5889227526443186 6 54 3.12394024968048 6 55 2.2862599381034663 6 56 1.1802143341597002 7 1 0.9122396954927723 7 2 1.5267001746648319 7 3 2.23478394489981 7 4 0.5870969500044652 7 5 1.277038397346999 7 6 0.8479444610572955 7 7 0.8722769958372467 7 8 0.36528615159615185 7 9 0.25529675662522777 7 10 1.190081087030407 7 11 1.3545392251031032 7 12 2.9877422274753584 7 13 3.2102104512266223 7 14 3.7654818412287554 7 15 3.9854308844682365 7 16 4.121128076243124 7 17 4.1323276852979305 7 18 4.070831838978442 7 19 3.411665217912706 7 20 3.242388330440086 7 21 1.9007764636398008 7 22 2.7765923818336944 7 23 2.042694058493595 7 24 1.833304162902678 7 25 1.7041962727273514 7 26 0.5121793420517022 7 27 0.6500196218173713 7 28 1.451026568329901 7 29 1.6193397475081 7 30 1.2792537298011055 7 31 0.41106499150624387 7 32 1.2452831795927821 7 33 0.14531218249307898 7 34 -0.24204667880898256 7 35 0.6887378747435875 7 36 0.5369371069623057 7 37 0.8713286390776602 7 38 0.4916517983154094 7 39 0.9819905095573169 7 40 3.0180629535376404 7 41 -2.7036539307155323 7 42 1.286357094657447 7 43 2.875158870619593 7 44 1.7902064125294181 7 45 0.6559004549370151 7 46 2.030015409210346 7 47 1.8726895632383034 7 48 1.8099452422929616 7 49 4.706679414739881 7 50 1.9959698121117952 7 51 3.2206210161081827 7 52 2.939711390731887 7 53 2.468730444952012 7 54 4.389004352244584 7 55 3.3288881432316693 7 56 4.416111770057135 8 1 0.6232101596277886 8 2 1.717060447303588 8 3 2.143255220459693 8 4 1.2873566059603192 8 5 1.2585379104920804 8 6 0.9222709784217855 8 7 0.6257661227440963 8 8 0.8865454829820649 8 9 1.535400619007575 8 10 0.17644914934783174 8 11 1.5053019644734391 8 12 2.93564875133125 8 13 3.0253678676498623 8 14 3.0704153043900675 8 15 4.099192650128735 8 16 4.123400065856892 8 17 3.885167672315127 8 18 3.6899490087285187 8 19 3.0786564545242 8 20 3.0259001772430736 8 21 1.9441065512736841 8 22 1.0028176333754004 8 23 1.106472052651334 8 24 2.1348296416433428 8 25 1.7616451529610444 8 26 1.0191575958654013 8 27 1.080399368652813 8 28 0.7200950525882561 8 29 0.014828225275060447 8 30 0.7972673617387827 8 31 -0.3693634408209192 8 32 0.14239450708384993 8 33 -1.1227825919308145 8 34 -1.756490041353615 8 35 -1.4060365491447655 8 36 -1.1294049897595393 8 37 -1.2615827533274029 8 38 -1.5945215220351265 8 39 -0.41901565727163614 8 40 2.0587964816129407 8 41 -3.4821349433737616 8 42 0.8537053582082414 8 43 2.6687323857120973 8 44 2.380923712107478 8 45 1.0197433630837232 8 46 3.002751533845206 8 47 2.3715535684314197 8 48 1.360253583740544 8 49 5.0747417321692865 8 50 1.8400139536242897 8 51 3.123899172550896 8 52 3.381613370608548 8 53 2.8333847779607737 8 54 4.375047844617189 8 55 3.68072196341992 8 56 3.2270497771976743 9 1 1.052932336185414 9 2 2.422304570269226 9 3 2.913105623221483 9 4 1.842674705582219 9 5 1.5817337020423068 9 6 1.5768821899950183 9 7 1.2031128266730775 9 8 1.2590590453690496 9 9 1.6562291575576324 9 10 1.9832878802371994 9 11 2.8751852457491225 9 12 4.145084884818019 9 13 3.9972567382729096 9 14 4.0370436127905265 9 15 4.764818163855516 9 16 4.651264606379655 9 17 5.2430502960205345 9 18 4.9940752622218625 9 19 3.7792975855450734 9 20 4.243720331772089 9 21 3.3887884516517843 9 22 3.3756599809012897 9 23 3.7305062463057865 9 24 3.7317390613375707 9 25 3.892008460539543 9 26 2.5245003543727123 9 27 2.416253388051139 9 28 2.941203058506389 9 29 1.7200723482406985 9 30 2.267265717813089 9 31 2.0948811753224277 9 32 2.493701428011029 9 33 2.160630197811095 9 34 1.7662783295160267 9 35 2.2015583875640985 9 36 3.0387686087938093 9 37 2.916783184532207 9 38 3.2294140360776495 9 39 3.250555278122082 9 40 6.547699983174674 9 41 3.172969445907846 9 42 4.769207577507931 9 43 5.437596433057159 9 44 5.612717235040243 9 45 4.5813083803449395 9 46 4.473571852766792 9 47 4.880348570897311 9 48 5.037550969898692 9 49 7.355031063091527 9 50 5.662469978611963 9 51 4.956218751705919 9 52 5.421396372416865 9 53 6.354344830566394 9 54 6.299427262667493 9 55 5.188695003038529 9 56 6.2983961523415175 10 1 0.6358683874758952 10 2 1.413262978949156 10 3 1.8267995242571615 10 4 0.717736352795761 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2.9410544910978444 116 30 2.9992056528780253 116 31 2.5384688376600906 116 32 2.773328051387651 116 33 2.6689500030058966 116 34 1.3981776801653751 116 35 1.8265583875640985 116 36 2.3490364659366634 116 37 2.5744536390776602 116 38 2.262084490623103 116 39 2.7712533300701345 116 40 7.736011671486359 116 41 3.8900960692844677 116 42 -1.5493800848297319 116 43 -5.620033437072713 116 44 -9.808751920803914 116 45 -0.7880098014732404 116 46 -4.811330744635807 116 47 -3.529554026505288 116 48 -4.496945783348064 116 49 -6.107022508337042 116 50 -5.121418008401026 116 51 -3.1860296249174596 116 52 -2.6031571990117044 116 53 -2.4423272473556814 116 54 -7.463559750319519 116 55 -1.6887400618965351 116 56 -0.22291066584029906 117 1 -0.2615191697554593 117 2 1.3949489471689276 117 3 1.557206203492278 117 4 -0.1136668252273978 117 5 0.4750744038936432 117 6 0.4218669924266294 117 7 -0.04889048916002281 117 8 -0.07224794551240876 117 9 -0.0845832215526201 117 10 0.772732478192431 117 11 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0.9081622338865074 118 32 -3.5999030806878203 118 33 1.2111080218738195 118 34 0.607493717901221 118 35 0.25816216114900215 118 36 2.612892598012138 118 37 2.522272035304077 118 38 2.5896198679815967 118 39 2.3779750281833394 118 40 5.669973935637302 118 41 4.796346069284468 118 42 -1.025205556527844 118 43 -8.111778720091582 118 44 -6.316711826464292 118 45 -0.6382456505298428 118 46 -7.878547725767884 118 47 -9.622714403863778 118 48 -7.923242953159384 118 49 -9.065454112110626 118 50 -5.984920366891593 118 51 -4.62795179472878 118 52 -5.0368246518418935 118 53 -4.874520643582098 118 54 -12.015446542772349 118 55 -4.536617420387103 118 56 -1.8782762318780364 119 1 -0.08109363784056 119 2 1.5451617131263689 119 3 1.7040147141305795 119 4 0.6076097705172856 119 5 0.5154999358085419 119 6 0.01973933285216134 119 7 0.2779180214782784 119 8 0.004773331083335108 119 9 0.22350188483036248 119 10 0.19315801010732514 119 11 -4.795204364640487 119 12 -11.307642387909254 119 13 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120 33 -1.6394381548888433 120 34 -2.962019688255676 120 35 -3.6668626650674803 120 36 -2.5777727445896517 120 37 -0.6286713609223398 120 38 -2.237093140955846 120 39 -1.434338775193023 120 40 1.8412748293810957 120 41 -3.598390772820796 120 42 -6.972077453250783 120 43 -13.064112384441135 120 44 -11.779969026067072 120 45 -10.46893085410482 120 46 -16.41330442884633 120 47 -16.87494876334739 120 48 -14.00516946755859 120 49 -17.663765929389676 120 50 -13.429806166295762 120 51 -11.973036203864828 120 52 -12.044769041116968 120 53 -12.986735142092524 120 54 -14.634612381898465 120 55 -12.16571374610706 120 56 -9.598733034261352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 140.0 5 41.0 6 44.0 7 83.0 8 99.0 9 119.0 10 85.0 11 98.0 12 105.0 13 112.0 14 131.0 15 183.0 16 215.0 17 292.0 18 364.0 19 461.0 20 549.0 21 659.0 22 817.0 23 1012.0 24 1136.0 25 1287.0 26 1382.0 27 1420.0 28 1668.0 29 2105.0 30 2440.0 31 2838.0 32 3177.0 33 3654.0 34 4285.0 35 5106.0 36 6174.0 37 7356.0 38 6236.0 39 3037.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.43333616226766 1.303572944071968 0.20877535432402614 0.054315539336332 8 97.38436730883477 2.0062802342357635 0.3683272511245014 0.24102520580497327 9 91.3638292455232 4.93083255537639 2.6580667062717476 1.0472714928286515 10 45.13451582788763 35.36450818976491 10.907239242977171 8.593736739370279 11 33.3090044980056 31.088856827633034 18.01578545361962 17.586353220741746 12 34.02189595179496 16.596791988457948 22.83628956971909 26.545022490028007 13 23.58203887615652 17.703081232493 26.225277989983876 32.489601901366605 14 24.61554129748447 20.271921784295756 29.11022846861527 26.00230844960451 15 28.706994779000354 18.12724273396711 26.055679325181547 27.11008316185099 16 30.590932246561387 20.062829003226355 24.98217014773306 24.3640686024792 17 28.159212424679623 22.646185182041926 24.2450988712552 24.949503522023253 18 27.81342295549445 22.936823165970736 23.6140815425875 25.635672335947312 19 28.326624887484925 26.310694451520867 21.76423634109475 23.598444319899457 20 28.145279108483674 24.039343593925185 23.071042707165425 24.744334590425712 21 27.110243571246713 22.315199864211152 26.01205126029025 24.56250530425189 22 26.882797250275818 21.785623355681917 25.338199100398878 25.993380293643387 23 26.52295680217262 24.628702367818043 23.793600950521938 25.054739879487396 24 26.640526869674442 23.83304477713277 25.890280748209253 23.636147604983535 25 26.801778864107 22.767084224462778 26.129612655735478 24.301524255694744 26 24.242475682832847 21.380434229065166 30.277207217912373 24.099882870189614 27 25.4806712412528 18.80392689720769 30.58801549018276 25.127386371356753 28 23.882574792314358 22.51669130013761 31.13670726942222 22.464026638125816 29 25.776872134488027 20.76413652572593 32.502971642044486 20.956019697741553 30 23.097032661098662 18.615468187682488 37.241121749168194 21.046377402050656 31 23.11492945311306 20.225138801636756 36.39404383924478 20.2658879060054 32 25.16054500356767 17.926675953926132 36.63823859196086 20.274540450545345 33 23.529111858876313 19.185275823391454 38.853200095170116 18.432412222562117 34 20.563413764412218 19.366286868961303 42.396970674636194 17.673328691990285 35 20.6037658963954 20.415301288690426 43.22121670034127 15.7597161145729 36 21.14574843792448 21.400434664493346 40.45096441184461 17.00285248573757 37 21.963077550742867 18.876007207697278 41.00227790432802 18.158637337231838 38 22.013370302958137 19.938081548641705 40.7573102896899 17.29123785871026 39 22.163081370631748 18.969475384746197 39.30278037581838 19.564662868803673 40 15.875401325227134 14.760229523874582 36.617938383769385 32.746430767128906 41 14.945870602520909 14.685912674659232 34.086983290862136 36.28123343195772 42 19.730179766880756 17.543412512318618 41.62503823019676 21.101369490603865 43 16.784882039767993 15.538092565188549 42.441870354305934 25.235155040737528 44 16.95993179880648 16.257459505541348 40.09548167092924 26.687127024722933 45 20.421936624507005 19.311845505235958 41.76356589147287 18.502651978784172 46 19.65554762147642 18.97888401509742 41.09626304872658 20.269305314699583 47 17.625282320376314 20.113097967293292 41.91757094095471 20.344048771375686 48 19.937784728360644 20.104372067722853 42.631060039437 17.3267831644795 49 16.31859795079871 17.394343386126124 36.74753226383893 29.539526399236237 50 19.129341398762996 20.03670223611772 41.78787466866037 19.046081696458913 51 19.550496801415544 21.187219273172726 40.804069688308154 18.45821423710358 52 18.53324497459686 20.325907801056907 41.28222120269834 19.85862602164789 53 18.68126264257424 20.535790171514048 41.79571299869112 18.98723418722059 54 17.222316720530703 20.326586154107844 40.14968532063276 22.301411804728698 55 18.572181691410304 21.381562165195653 39.86871418124925 20.177541962144787 56 20.7197864780783 21.092089828808458 39.06465158186423 19.123472111249 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 7.5 7 12.0 8 22.0 9 32.0 10 67.5 11 103.0 12 150.0 13 228.0 14 259.0 15 269.0 16 279.0 17 358.0 18 437.0 19 572.0 20 707.0 21 737.5 22 873.0 23 978.0 24 1182.0 25 1386.0 26 1602.0 27 1818.0 28 2154.5 29 2532.0 30 2573.0 31 2389.5 32 2206.0 33 1955.0 34 1704.0 35 1525.0 36 1346.0 37 1197.5 38 1022.0 39 995.0 40 990.5 41 986.0 42 992.5 43 999.0 44 1061.5 45 1124.0 46 1315.0 47 1546.0 48 1586.0 49 1630.5 50 1675.0 51 1809.5 52 1944.0 53 2130.0 54 2392.5 55 2469.0 56 2629.0 57 2789.0 58 3017.0 59 3245.0 60 3524.5 61 3804.0 62 3739.5 63 3342.5 64 3010.0 65 2792.0 66 2574.0 67 2227.5 68 1881.0 69 1623.5 70 1366.0 71 1160.0 72 811.5 73 669.0 74 574.0 75 479.0 76 370.5 77 262.0 78 193.5 79 97.5 80 70.0 81 57.5 82 45.0 83 29.0 84 13.0 85 9.0 86 5.0 87 3.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.016973606042603753 14 0.001697360604260375 15 0.19349910888568275 16 0.04243401510650938 17 0.0 18 0.001697360604260375 19 0.057710260544852746 20 0.08317066960875838 21 0.0 22 0.0 23 0.0 24 0.001697360604260375 25 0.001697360604260375 26 0.01018416362556225 27 0.06619706356615462 28 0.08826275142153951 29 0.04243401510650938 30 0.011881524229822625 31 0.030552490876686753 32 0.08996011202579988 33 0.12390732411100738 34 0.040736654502249 35 0.030552490876686753 36 0.032249851480947125 37 0.1510650937791734 38 0.217262157345328 39 0.18670966646864126 40 0.6093524569294747 41 0.7536281082916065 42 0.10353899685988288 43 0.2104727149282865 44 0.4498005601289994 45 0.15445981498769412 46 0.1646439786132564 47 0.0475260969192905 48 0.14767037257065263 49 0.43791903589917675 50 0.10693371806840364 51 0.23593312399219213 52 0.108631078672664 53 0.14597301196639226 54 0.2121700755325469 55 0.190104387677162 56 0.15615717559195452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 58915.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.46465246541628 #Duplication Level Percentage of deduplicated Percentage of total 1 86.3067926225821 65.13112110667912 2 8.092667566351777 12.214206908257658 3 2.5528565002249213 5.779512857506577 4 1.158344579397211 3.496562844776373 5 0.6162843004948267 2.325384027836714 6 0.3733693207377418 1.6905711618433337 7 0.20467836257309943 1.081218704913859 8 0.1484480431848853 0.8962063990494781 9 0.0832208726945569 0.5652210812187048 >10 0.4408457040035988 5.378935754901129 >50 0.020242914979757085 1.026903165577527 >100 0.002249212775528565 0.4141559874395315 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 244 0.4141559874395315 No Hit TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 91 0.15445981498769412 No Hit TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.1289994059237885 No Hit TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 72 0.12220996350674701 No Hit TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 69 0.11711788169396589 No Hit TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 64 0.108631078672664 No Hit TAAGGGGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG 62 0.10523635746414325 No Hit TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 59 0.10014427565136212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0016973606042603752 0.0 0.0 0.0 0.0 8 0.0033947212085207504 0.0 0.0 0.0 0.0 9 0.005092081812781125 0.0 0.0 0.0 0.0 10 0.008486803021301876 0.0 0.0 0.0 0.0 11 0.01018416362556225 0.0 0.0 0.0 0.0 12 0.016973606042603753 0.0 0.0 0.0 0.0 13 0.0203683272511245 0.0 0.0 0.0 0.0 14 0.022065687855384878 0.0 0.0 0.0 0.0 15 0.030552490876686753 0.0 0.0 0.0 0.0 16 0.033947212085207505 0.0 0.0 0.0 0.0 17 0.03564457268946788 0.0 0.0 0.0 0.0 18 0.03564457268946788 0.0 0.0 0.0 0.0 19 0.040736654502249 0.0 0.0 0.0 0.0 20 0.044131375710769756 0.0 0.0 0.0 0.0 21 0.0475260969192905 0.0 0.0 0.0 0.0 22 0.04922345752355088 0.0 0.0 0.0 0.0 23 0.04922345752355088 0.0 0.0 0.0 0.0 24 0.061104981753373505 0.0 0.0 0.0 0.0 25 0.06619706356615462 0.0 0.0 0.0 0.0 26 0.07128914537893576 0.0 0.0 0.0 0.0 27 0.07807858779597726 0.0 0.0 0.0 0.0 28 0.07807858779597726 0.0 0.0 0.0 0.0 29 0.08317066960875838 0.0 0.0 0.0 0.0 30 0.08317066960875838 0.0 0.0 0.0 0.0 31 0.08317066960875838 0.0 0.0 0.0 0.0 32 0.08656539081727913 0.0 0.0 0.0 0.0 33 0.08656539081727913 0.0 0.0 0.0 0.0 34 0.08656539081727913 0.0 0.0 0.0 0.0 35 0.08656539081727913 0.0 0.0 0.0 0.0 36 0.08826275142153951 0.0 0.0 0.0 0.0 37 0.08996011202579988 0.0 0.0 0.0 0.0 38 0.08996011202579988 0.0 0.0 0.0 0.0 39 0.08996011202579988 0.0 0.0 0.0 0.0 40 0.08996011202579988 0.0 0.0 0.0 0.0 41 0.08996011202579988 0.0 0.0 0.0 0.0 42 0.09335483323432063 0.0 0.0 0.0 0.0 43 0.095052193838581 0.0 0.0 0.0 0.0 44 0.09674955444284138 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCAAA 15 0.008255913 50.206158 36 GCAAGAA 15 0.0083119515 50.12041 46 CAACTCT 15 0.0083119515 50.12041 48 ACCCGCT 15 0.0083119515 50.12041 34 GAATGGC 15 0.0083119515 50.12041 44 AACTCTA 15 0.0083119515 50.12041 49 CACCTGC 15 0.0083119515 50.12041 43 CTTGTGC 15 0.0083119515 50.12041 49 CAGGCAC 15 0.0083119515 50.12041 49 GCATATT 15 0.0083119515 50.12041 49 AAGCATA 15 0.0083119515 50.12041 47 CCAAAAC 40 2.5538611E-9 50.120407 43 AATTCGT 20 4.1195587E-4 50.077644 13 GAAGTTA 15 0.0083400775 50.077644 9 TGTTCAC 15 0.0083400775 50.077644 11 GATGAGC 15 0.0083400775 50.077644 13 GAATTCG 20 4.1195587E-4 50.077644 12 AGATGGA 25 2.0442378E-5 50.077644 13 GGAATTC 30 1.0198164E-6 50.077644 11 TGTACGC 15 0.0083400775 50.077644 10 >>END_MODULE