FastQCFastQC Report
Fri 17 Jun 2016
SRR1526253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59223
Sequences flagged as poor quality0
Sequence length56
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC980.16547625078094658No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.13845972004120022No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT750.12663998784256117No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC670.11313172247268799No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.10637758978775137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGATC150.00820718650.2815743
TTGATCA150.00820718650.2815744
CATTGAT150.00826275250.1959142
TCTGACA150.00829063950.1531945
TGGGGAT150.00834662550.0679715
GAAGGTA150.00834662550.0679714
GGTTGGG150.008402890549.9830412
TGGCACC204.1583763E-449.9830412
GTTTGAA150.008402890549.9830410
AGAAGCA150.008402890549.9830450
GGCCCCC150.008402890549.9830411
AATGGTC150.008402890549.9830410
GCATGGA150.008402890549.9830412
ATCGAGT150.008402890549.9830410
GTTATCA150.008402890549.9830412
TCGAGTT150.008402890549.9830411
CGGTAGT150.008402890549.9830412
GGAAAAG204.1583763E-449.9830450
AAGCCGT252.0673831E-549.98303610
AAGCTGT252.0673831E-549.98303610
Produced by FastQC (version 0.11.5)