FastQCFastQC Report
Fri 17 Jun 2016
SRR1526252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16853
Sequences flagged as poor quality0
Sequence length56
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2851.6910935738444193No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1160.6883047528629918No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG790.4687592713463478No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG530.3144840681184359No Hit
ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.2314128048418679No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG280.16614252655313594No Hit
ATTTGCGGGGGGCAGTGGTGGCACACGCCTTTAATCCTAGCACTTGGGATAAAGAG270.16020886489052394No Hit
ATTTGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC260.15427520322791194No Hit
ATTTGCGGGCTATCGCAGATCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.13647421824007597No Hit
ATTTGCGGGATTCTATCTTTATTTAGTGCATTTGTTGTTCTGATTATTATGTGTCG220.13054055657746394No Hit
ATTTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA200.11867323325223997No Hit
ATTTGCGGGAAGCATGGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT190.11273957158962795No Hit
ATTTGCGGGTGGCACATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10680590992701597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTC550.050.33033440
AGCCCCC150.00807383750.33033438
ATCCTAG150.00807383750.33033434
AAACCAA150.00807383750.33033437
TCCTAGC150.00807383750.33033435
TATGCTT600.050.33033435
AAGAGCC150.00807383750.33033435
AGAGCCC150.00807383750.33033436
ATGCTTG600.050.33033436
CTAGCAC150.00807383750.33033437
TGGTTGA750.050.3303314
GGTTGAT800.050.3303315
ATATGCT700.050.3303334
ATATGCC203.9353315E-450.3303334
ACATGGT150.00817053350.1796411
GTCGCAG150.00817053350.1796412
GAAGTGG309.62531E-750.179649
ACCTGGT700.050.1796411
GCAGTGG354.7451977E-850.1796412
GAACATG150.00817053350.179649
Produced by FastQC (version 0.11.5)