##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526252_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16853 Sequences flagged as poor quality 0 Sequence length 56 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.87159556162108 39.0 38.0 39.0 35.0 40.0 2 37.33038628137424 39.0 38.0 39.0 34.0 40.0 3 37.40337031982436 39.0 38.0 39.0 35.0 40.0 4 37.30487153622501 39.0 38.0 39.0 34.0 40.0 5 36.53118139203703 39.0 36.0 39.0 31.0 40.0 6 37.04070491900552 39.0 38.0 39.0 33.0 40.0 7 37.26950691271584 39.0 38.0 39.0 34.0 40.0 8 37.28125556280781 39.0 38.0 39.0 34.0 40.0 9 37.31228861330327 39.0 38.0 39.0 34.0 40.0 10 36.614430665163475 39.0 36.0 39.0 32.0 40.0 11 35.62534860262268 38.0 35.0 39.0 29.0 40.0 12 33.743428469708654 38.0 32.0 39.0 20.0 40.0 13 33.61543938764612 38.0 32.0 39.0 20.0 40.0 14 33.840977867442 38.0 33.0 39.0 21.0 40.0 15 33.71079333056429 38.0 33.0 39.0 19.0 40.0 16 33.045155165252474 38.0 31.0 39.0 19.0 40.0 17 33.16614252655314 37.0 31.0 39.0 20.0 40.0 18 32.642437548211 37.0 30.0 39.0 18.0 40.0 19 33.43760754761764 37.0 31.0 39.0 23.0 40.0 20 33.43915029964992 37.0 31.0 39.0 23.0 40.0 21 34.25704622322435 38.0 33.0 39.0 24.0 40.0 22 34.031270396961965 38.0 33.0 39.0 23.0 40.0 23 34.32516465911114 38.0 33.0 39.0 24.0 40.0 24 33.9445796000712 38.0 33.0 39.0 23.0 40.0 25 34.120928024684034 38.0 33.0 39.0 23.0 40.0 26 34.88292885539666 38.0 35.0 39.0 27.0 40.0 27 34.73132379991693 38.0 34.0 39.0 26.0 40.0 28 34.64237821159438 38.0 34.0 39.0 26.0 40.0 29 34.51741529697976 38.0 34.0 39.0 25.0 40.0 30 34.32937755889159 38.0 33.0 39.0 25.0 40.0 31 34.75203227911945 38.0 35.0 39.0 27.0 40.0 32 34.34605114816353 38.0 34.0 39.0 25.0 40.0 33 34.564172550881146 38.0 34.0 39.0 27.0 40.0 34 34.4979528867264 38.0 34.0 39.0 27.0 40.0 35 34.378448940841395 38.0 34.0 39.0 26.0 40.0 36 33.582566902035246 36.0 33.0 39.0 25.0 40.0 37 33.29490298463182 36.0 32.0 39.0 24.0 40.0 38 33.01376609505726 36.0 32.0 39.0 23.0 40.0 39 32.78721889277873 36.0 32.0 39.0 21.0 40.0 40 29.367709013232066 35.0 23.0 38.0 6.0 39.0 41 25.850471726102178 31.0 13.0 38.0 4.0 39.0 42 30.337447338752746 35.0 29.0 38.0 4.0 39.0 43 28.660001186732334 33.0 25.0 38.0 4.0 39.0 44 28.59918115469056 33.0 24.0 38.0 4.0 39.0 45 29.936450483593426 34.0 28.0 38.0 4.0 39.0 46 29.604521450186912 35.0 27.0 38.0 4.0 39.0 47 29.025218062066102 35.0 26.0 38.0 4.0 39.0 48 29.222690322197828 35.0 26.0 38.0 4.0 39.0 49 26.54014122114757 33.0 13.0 38.0 4.0 39.0 50 28.809707470480035 34.0 26.0 38.0 4.0 39.0 51 29.08550406455824 35.0 27.0 39.0 4.0 40.0 52 28.82964457366641 35.0 26.0 39.0 4.0 40.0 53 28.429359757906603 35.0 23.0 39.0 4.0 40.0 54 27.395300539963213 34.0 16.0 39.0 4.0 40.0 55 27.867738681540377 34.0 22.0 39.0 4.0 40.0 56 26.994066338337387 34.0 17.0 39.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.8588724636932881 1 2 1.8102227300133578 1 3 1.7048413911509854 1 4 1.6408153652190123 1 5 2.6254339184064435 1 6 2.1743453723420956 1 7 1.733674772624454 1 8 1.771296355397567 1 9 1.7254427586231103 1 10 1.6590883069315652 1 11 -1.1318609299197462 1 12 -9.040254220975395 1 13 -8.533397814174123 1 14 -2.174191789978174 1 15 -6.08992863917128 1 16 -22.543235564057557 1 17 -4.706753691326444 1 18 -13.947250616006219 1 19 -5.010243825439829 1 20 -5.373018365857558 1 21 -4.473086324701846 1 22 -1.441085190408721 1 23 -2.36714445473115 1 24 -2.0388399694417245 1 25 -2.562279794680734 1 26 2.893816017416242 1 27 3.924154329044427 1 28 4.043701982457392 1 29 2.8433707068788365 1 30 3.5408997249954908 1 31 4.012452784888538 1 32 4.174239346946187 1 33 2.832495655925044 1 34 2.8249769341998388 1 35 3.066182300721593 1 36 3.802679894802779 1 37 4.315381659491187 1 38 4.448637584959766 1 39 3.190754488445261 1 40 7.19085699721245 1 41 -6.203488940769134 1 42 -7.808621435932782 1 43 -13.6154692667655 1 44 -20.3330810513446 1 45 -9.05552028137554 1 46 -13.624729129179778 1 47 -21.61161646603596 1 48 -11.225320135095096 1 49 -18.033757886864322 1 50 -11.063024793182123 1 51 -7.536864609082304 1 52 -7.81977429380332 1 53 -7.096682345360755 1 54 -5.713595666280455 1 55 -6.579677571432402 1 56 -1.4517965711690515 2 1 1.2781032329240531 2 2 2.5756073453979695 2 3 2.5086875449971373 2 4 2.644661519065167 2 5 3.171587764560293 2 6 2.9781915261882475 2 7 2.3836747726244525 2 8 2.575142509243719 2 9 2.1446735278538753 2 10 2.674472922316177 2 11 2.2181390700802552 2 12 -1.163331144052318 2 13 -0.5757055064818175 2 14 4.171962056175673 2 15 4.1485328992902595 2 16 4.475995205173209 2 17 4.654784770212014 2 18 0.30659553783993587 2 19 4.301294636098632 2 20 4.096212403373208 2 21 4.026913675298154 2 22 4.881991732668197 2 23 4.048240160653464 2 24 3.4380831074813543 2 25 4.318489436088498 2 26 4.351508325108547 2 27 3.9933850982751906 2 28 3.716778905534312 2 29 4.12029378380192 2 30 2.3985920326877945 2 31 1.958606631042386 2 32 2.8934701161769567 2 33 3.251726425155809 2 34 2.217284626507535 2 35 1.9238746084138967 2 36 3.7796029717258577 2 37 4.134612428721951 2 38 4.5563298926520694 2 39 4.790754488445263 2 40 6.452395458750907 2 41 -4.672719709999903 2 42 -4.3663137436250885 2 43 -11.28470003599627 2 44 -12.848465666729217 2 45 -3.7593664352216933 2 46 -6.993959898410548 2 47 -16.83853954295904 2 48 -6.271473981248942 2 49 -11.787604040710473 2 50 -5.197640177797506 2 51 0.22082769861000173 2 52 0.2879180138889872 2 53 -0.41591311459152536 2 54 0.8210197183349273 2 55 0.17801473625990383 2 56 2.175126505754026 3 1 0.7588724636932866 3 2 2.5852227300133563 3 3 2.229841391150984 3 4 1.8658153652190137 3 5 2.908767251739782 3 6 2.65767870567543 3 7 2.425341439291117 3 8 2.1712963553975655 3 9 2.500442758623109 3 10 2.3924216402648995 3 11 2.8431390700802552 3 12 5.384745779024605 3 13 3.366602185825876 3 14 4.200808210021826 3 15 4.443404694162055 3 16 4.956764435942443 3 17 5.3182463086735545 3 18 5.902749383993783 3 19 4.048089507893508 3 20 4.618648300809106 3 21 4.4852470086314895 3 22 4.750581476257942 3 23 4.391188878602186 3 24 4.236160030558274 3 25 3.5460535386526004 3 26 2.8771493507495762 3 27 3.7658209957110884 3 28 3.5437019824573923 3 29 1.7933707068788394 3 30 3.340899724995488 3 31 1.6541194515552036 3 32 3.457572680279526 3 33 2.3574956559250424 3 34 1.7749769341998416 3 35 3.74118230072159 3 36 4.619346561469442 3 37 4.6153816594911845 3 38 4.998637584959763 3 39 6.290754488445263 3 40 9.324190330545783 3 41 10.929844392564203 3 42 6.233045230733882 3 43 2.1928640665678323 3 44 5.141918948655398 3 45 6.852813051957796 3 46 4.608604204153558 3 47 -2.4282831327026315 3 48 5.324679864904905 3 49 5.74124211313568 3 50 4.228641873484545 3 51 3.1214687242510273 3 52 1.9385590395300127 3 53 2.8949843213059125 3 54 3.769737667052876 3 55 2.5786557619009294 3 56 5.639870095497617 4 1 1.0997815546023801 4 2 2.2784045481951765 4 3 2.4002959366055308 4 4 2.581724456128107 4 5 2.7799793729518996 4 6 1.7788908268875474 4 7 2.110947499897179 4 8 1.5576599917611986 4 9 2.193624576804929 4 10 1.4681792160224703 4 11 2.3545027064438884 4 12 4.759745779024605 4 13 5.252965822189509 4 14 5.2348991191127325 4 15 5.291889542646899 4 16 5.206764435942443 4 17 6.011428126855375 4 18 4.152749383993783 4 19 4.885210720014719 4 20 5.1906179977788085 4 21 4.345095493479974 4 22 2.549823900500371 4 23 4.11467372708703 4 24 4.179341848740094 4 25 3.860447478046538 4 26 3.816543290143514 4 27 3.405972510862604 4 28 3.2937019824573923 4 29 4.407007070515206 4 30 3.8181724522682146 4 31 3.6124527848885393 4 32 2.987875710582557 4 33 3.573404746834136 4 34 2.479522388745295 4 35 3.752545937085223 4 36 4.884498076620957 4 37 5.3653816594911845 4 38 5.157728494050669 4 39 5.381663579354356 4 40 8.536311542666994 4 41 12.096511059230867 4 42 6.123196745885398 4 43 9.620894369598133 4 44 9.039646221382672 4 45 7.71266153680628 4 46 8.093452689002042 4 47 8.056565352145856 4 48 8.574679864904905 4 49 12.320787567681133 4 50 8.096066115908783 4 51 8.458589936372242 4 52 7.3438620698330475 4 53 8.535135836457425 4 54 8.572767970083177 4 55 7.00668606493123 4 56 6.416385247012766 5 1 0.6688724636932832 5 2 1.9802227300133595 5 3 2.2748413911509857 5 4 2.4108153652190154 5 5 1.6054339184064474 5 6 2.304345372342091 5 7 1.8636747726244494 5 8 2.4212963553975655 5 9 1.6154427586231108 5 10 2.419088306931563 5 11 3.098139070080258 5 12 4.579745779024606 5 13 4.236602185825873 5 14 4.765808210021824 5 15 3.970071360828719 5 16 4.8867644359424425 5 17 5.513246308673555 5 18 4.892749383993785 5 19 5.119756174560173 5 20 3.90698163414244 5 21 3.866913675298157 5 22 4.098914809591278 5 23 3.812855545268853 5 24 4.561160030558277 5 25 3.7677202053192644 5 26 2.683816017416241 5 27 0.9041543290444238 5 28 1.8137019824573954 5 29 2.3433707068788365 5 30 2.070899724995485 5 31 2.4324527848885396 5 32 2.784239346946187 5 33 2.8024956559250427 5 34 1.6849769341998382 5 35 3.1761823007215924 5 36 4.542679894802781 5 37 5.185381659491185 5 38 4.968637584959762 5 39 4.630754488445266 5 40 8.69085699721245 5 41 9.736511059230867 5 42 6.5413785640672195 5 43 9.324530733234504 5 44 7.126918948655398 5 45 5.39447971862446 5 46 6.335270870820224 5 47 5.838383533964038 5 48 5.394679864904905 5 49 8.50624211313568 5 50 4.566975206817876 5 51 5.973135390917697 5 52 5.600225706196678 5 53 4.453317654639246 5 54 6.736404333719541 5 55 4.210322428567597 5 56 4.71820342883095 6 1 1.6338724636932866 6 2 2.7518893966800206 6 3 2.8131747244843197 6 4 2.6991486985523494 6 5 3.3671005850731106 6 6 3.1160120390087584 6 7 2.717008105957788 6 8 2.6712963553975655 6 9 2.7087760919564445 6 10 2.475754973598228 6 11 3.7598057367469195 6 12 5.593079112357941 6 13 5.741602185825876 6 14 4.700808210021826 6 15 5.901738027495384 6 16 6.2900977692757785 6 17 5.6932463086735545 6 18 7.027749383993783 6 19 4.673089507893508 6 20 5.868648300809106 6 21 4.943580341964818 6 22 4.458914809591278 6 23 4.099522211935515 6 24 4.736160030558274 6 25 4.587720205319265 6 26 4.502149350749576 6 27 3.68248766237776 6 28 3.6687019824573923 6 29 2.6683707068788394 6 30 3.7575663916621522 6 31 3.1124527848885393 6 32 3.2909060136128545 6 33 2.315828989258378 6 34 4.691643600866506 6 35 2.99118230072159 6 36 4.494346561469442 6 37 3.9903816594911845 6 38 3.873637584959763 6 39 5.582421155111927 6 40 7.907523663879111 6 41 5.554844392564199 6 42 5.108045230733882 6 43 7.192864066567836 6 44 6.391918948655398 6 45 6.436146385291124 6 46 7.233604204153558 6 47 7.405050200630701 6 48 7.741346531571569 6 49 10.282908779802344 6 50 8.728641873484541 6 51 7.288135390917695 6 52 7.938559039530016 6 53 7.728317654639245 6 54 9.228071000386208 6 55 5.5369890952342615 6 56 7.473203428830949 7 1 1.5088724636932866 7 2 2.2329500027406297 7 3 1.9002959366055308 7 4 1.763542637946287 7 5 1.7345248274973528 7 6 2.369799917796641 7 7 1.974583863533546 7 8 2.0576599917611986 7 9 2.0572609404412887 7 10 1.6499973978406572 7 11 3.2181390700802552 7 12 5.441563960842785 7 13 4.798420367644056 7 14 4.507626391840006 7 15 5.200980451737813 7 16 6.479491708669716 7 17 6.375064490491734 7 18 7.198203929448329 7 19 5.7488470836510785 7 20 6.0087998159606215 7 21 4.026913675298154 7 22 4.913460264136731 7 23 5.61467372708703 7 24 4.770250939649181 7 25 4.405902023501085 7 26 3.816543290143514 7 27 1.8605179654080573 7 28 3.384611073366486 7 29 4.452461615969746 7 30 3.545445179540941 7 31 2.5215436939794458 7 32 2.851512074218917 7 33 3.073404746834136 7 34 3.297704206927115 7 35 2.61618230072159 7 36 3.111770803893684 7 37 3.274472568582091 7 38 3.8395466758688563 7 39 3.6543908520816295 7 40 6.854493360848814 7 41 2.55105651377632 7 42 3.6686512913399447 7 43 5.939076187779953 7 44 5.448737130473578 7 45 5.258116082260827 7 46 3.1389072344565854 7 47 4.056565352145853 7 48 3.847407137632178 7 49 5.729878476772043 7 50 2.596066115908787 7 51 1.2313172090995117 7 52 1.8893166152875907 7 53 1.5805903819119713 7 54 3.4818588791740908 7 55 3.5521406103857807 7 56 1.5982034288309492 8 1 0.78159973642056 8 2 2.142040911831536 8 3 1.1730232093328041 8 4 1.945360819764467 8 5 2.1436157365882664 8 6 2.0970726450693675 8 7 2.156402045351726 8 8 1.9212963553975655 8 9 2.148170031350382 8 10 2.195451943295197 8 11 2.399957251898435 8 12 5.123382142660972 8 13 4.934784004007696 8 14 5.053080937294553 8 15 3.200980451737813 8 16 5.888582617760623 8 17 4.1932463086735545 8 18 5.3345675658119625 8 19 3.6124834472874454 8 20 3.736072543233348 8 21 3.1178227662072473 8 22 3.8225511732276445 8 23 3.023764636177944 8 24 3.497523666921907 8 25 2.360447478046538 8 26 2.771088744688967 8 27 2.1332452381353306 8 28 2.9300656188210255 8 29 2.3615525250606595 8 30 4.454536088631855 8 31 2.976089148524906 8 32 2.987875710582557 8 33 2.7552229286523158 8 34 2.2067951160180215 8 35 2.979818664357957 8 36 3.111770803893684 8 37 0.956290750400278 8 38 2.1577284940506694 8 39 2.199845397536169 8 40 5.172675179030627 8 41 -6.44894348622368 8 42 4.259560382249038 8 43 6.30271255141632 8 44 5.357828039564492 8 45 5.8035706277153665 8 46 4.3661799617293156 8 47 4.965656261236763 8 48 5.483770773995811 8 49 8.002605749499313 8 50 6.64152066136333 8 51 6.413135390917695 8 52 6.389316615287587 8 53 4.898772200093791 8 54 7.481858879174091 8 55 5.779413337658504 8 56 4.007294337921859 9 1 1.6041105589313815 9 2 2.6030798728705022 9 3 2.592936629246225 9 4 2.8241486985523494 9 5 3.2778148707873953 9 6 2.824345372342094 9 7 2.669389058338737 9 8 2.3736773077785145 9 9 2.184966568146919 9 10 2.3448025926458484 9 11 3.7419485938897807 9 12 5.450221969500795 9 13 3.4023164715401606 9 14 5.754379638593257 9 15 6.371976122733479 9 16 6.682954912132917 9 17 5.002770118197365 9 18 6.986082717327118 9 19 5.3159466507506465 9 20 6.255553062713872 9 21 4.265008913393395 9 22 3.887486238162708 9 23 4.8852364976497995 9 24 5.313540982939223 9 25 3.80200591960498 9 26 1.520006493606715 9 27 2.8908209957110884 9 28 1.8175115062669178 9 29 3.733846897355029 9 30 2.495661629757393 9 31 3.5886432610790138 9 32 4.2909060136128545 9 33 4.577733751163137 9 34 2.8583102675331773 9 35 4.473325157864444 9 36 4.083632275755157 9 37 5.8653816594911845 9 38 5.153399489721668 9 39 4.743135440826212 9 40 6.871809378164826 9 41 6.286987249707057 9 42 6.227092849781503 9 43 6.960721209424975 9 44 8.195490377226829 9 45 7.20400352814827 9 46 7.489556585105937 9 47 8.047907343487847 9 48 8.55087034109538 9 49 11.43767068456425 9 50 9.734594254436928 9 51 9.555992533774841 9 52 8.004035230006206 9 53 9.472365273686865 9 54 10.888785286100493 9 55 6.227465285710451 9 56 8.455346285973803 10 1 1.9088724636932852 10 2 2.9602227300133563 10 3 2.854841391150984 10 4 2.9908153652190137 10 5 3.6254339184064435 10 6 2.774345372342097 10 7 2.3336747726244553 10 8 2.771296355397567 10 9 2.8254427586231117 10 10 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1.760273433702821 115 34 1.191643600866506 115 35 1.61618230072159 115 36 1.8693465614694418 115 37 2.4209372150467416 115 38 2.9708598071819843 115 39 2.7351989328897055 115 40 4.990856997212447 115 41 5.207622170341978 115 42 1.4969341196227752 115 43 -3.6265803778766106 115 44 -3.1775254957890446 115 45 0.2278130519577921 115 46 -0.5025069069575565 115 47 -0.7616164660359637 115 48 -0.536431246206206 115 49 -11.800424553530986 115 50 -0.20191368207101235 115 51 0.1909131686954737 115 52 -0.5753298493588765 115 53 -0.5911267898051982 115 54 -8.285817888502677 115 55 -0.40745534921018134 115 56 3.8204256510531707 116 1 0.8898248446456662 116 2 2.1745084442990716 116 3 1.9738890101986044 116 4 2.205101079504729 116 5 2.563529156501687 116 6 2.062440610437335 116 7 1.3836747726244525 116 8 1.1832011173023247 116 9 1.5182999014802547 116 10 2.1067073545506148 116 11 -5.0675752156340295 116 12 -15.359301840023015 116 13 -14.264350195126504 116 14 -5.817048932835316 116 15 -3.3899286391712806 116 16 -2.174187945009937 116 17 -1.0924679770407302 116 18 -5.91867918743479 116 19 -1.49357715877316 116 20 -1.553970746809938 116 21 3.645961294345774 116 22 4.173200523876993 116 23 3.9328555452688505 116 24 4.170683840082084 116 25 3.4210535386526004 116 26 3.520006493606715 116 27 3.6527257576158547 116 28 2.0079876967431076 116 29 4.067180230688365 116 30 2.3051854392812032 116 31 2.5886432610790138 116 32 3.4337631564700004 116 33 3.2444004178298016 116 34 3.286881696104601 116 35 2.3304680150073054 116 36 3.5122037043265877 116 37 3.341572135681659 116 38 2.7724471087692883 116 39 2.0288497265405034 116 40 6.967047473402921 116 41 3.9536539163737245 116 42 -1.6300500073613549 116 43 -9.658326409622642 116 44 -8.375938194201744 116 45 -2.5579012337564926 116 46 -5.272348176798825 116 47 -3.6663783707978688 116 48 -7.1634153731903325 116 49 -10.990900744007178 116 50 -4.265405745563076 116 51 -3.1106741328918304 116 52 -3.376917150946177 116 53 -4.24192044059885 116 54 -7.777881380566171 116 55 -3.8201537619085926 116 56 -1.639891809264288 117 1 0.04733400215482675 117 2 1.3832996530902761 117 3 1.4317644680740642 117 4 1.2600461344497802 117 5 1.7100493030218331 117 6 1.5935761415728606 117 7 0.9221363110859926 117 8 1.4982194323206457 117 9 1.2985196817000286 117 10 1.2898575377007973 117 11 -2.320322468381285 117 12 -7.586408067129241 117 13 -6.960320891097201 117 14 -9.058807174593557 117 15 -8.15915940840205 117 16 -14.024004794826787 117 17 -8.806753691326445 117 18 -11.116481385236987 117 19 -3.929474594670598 117 20 -5.403787596626788 117 21 3.103836752221234 117 22 3.4589148095912776 117 23 2.163624776038084 117 24 3.1303907997890406 117 25 3.0877202053192647 117 26 2.9668929404931603 117 27 1.4549235598136576 117 28 2.9090865978420055 117 29 4.0818322453403795 117 30 3.052438186533948 117 31 3.2662989387346926 117 32 2.08577780848465 117 33 2.9440341174635023 117 34 2.832669241892148 117 35 2.3084899930292835 117 36 3.856526048648931 117 37 3.557689351798878 117 38 4.5563298926520694 117 39 5.406139103829876 117 40 7.490856997212447 117 41 6.01958798230779 117 42 1.941378564067218 117 43 -11.28470003599627 117 44 -11.54077335903691 117 45 3.2406335647783067 117 46 0.3906554862048388 117 47 0.7768450724255764 117 48 1.6900644802895215 117 49 -9.826065579172012 117 50 0.3023598222024937 117 51 1.0285200063023119 117 52 4.018687244658221 117 53 1.3148561161777046 117 54 -4.678980281665073 117 55 4.216476274721444 117 56 6.444357274984796 118 1 0.10887246369328807 118 2 1.0602227300133578 118 3 0.9548413911509854 118 4 0.8908153652190123 118 5 1.5254339184064492 118 6 1.3576787056754256 118 7 0.9170081059577839 118 8 1.021296355397567 118 9 0.9754427586231103 118 10 1.2590883069315666 118 11 -4.0485275965864105 118 12 -9.873587554308727 118 13 -11.15006448084079 118 14 -8.274191789978175 118 15 -5.5232619725046135 118 16 -14.793235564057557 118 17 -9.54008702465978 118 18 -8.347250616006217 118 19 -4.160243825439828 118 20 -3.439685032524224 118 21 1.9602470086314838 118 22 0.6589148095912805 118 23 2.399522211935519 118 24 1.7611600305582726 118 25 2.4877202053192633 118 26 0.643816017416242 118 27 1.9574876623777584 118 28 2.693701982457391 118 29 1.4767040402121694 118 30 1.524233058328818 118 31 0.04578611822186929 118 32 -4.242427319720477 118 33 -0.5175043440749576 118 34 0.5249769341998416 118 35 0.34951563405492436 118 36 1.7026798948027775 118 37 1.8653816594911845 118 38 2.6486375849597614 118 39 3.190754488445261 118 40 5.62419033054578 118 41 4.896511059230868 118 42 -0.3252881025994476 118 43 -9.448802600098833 118 44 -13.166414384677935 118 45 -1.738853614708873 118 46 -11.091395795846445 118 47 -13.42828313270263 118 48 -10.725320135095096 118 49 -13.33375788686432 118 50 -7.3796914598487895 118 51 -6.586864609082305 118 52 -6.453107627136653 118 53 -5.88001567869409 118 54 -15.863595666280457 118 55 -5.029677571432401 118 56 -1.6684632378357165 119 1 -0.09112753630671477 119 2 0.8602227300133549 119 3 0.7548413911509826 119 4 0.8908153652190123 119 5 1.7254339184064449 119 6 0.5576787056754284 119 7 0.45034143929112247 119 8 0.8212963553975641 119 9 0.7754427586231074 119 10 0.45908830693156233 119 11 -4.31519426325308 119 12 -9.473587554308729 119 13 -11.016731147507457 119 14 -16.340858456644842 119 15 -11.256595305837948 119 16 -13.326568897390892 119 17 -17.073420357993115 119 18 -15.813917282672884 119 19 -5.960243825439829 119 20 -8.906351699190893 119 21 1.4935803419648224 119 22 2.192248142924612 119 23 1.7995222119355176 119 24 2.0278266972249384 119 25 1.3543868719859304 119 26 3.0438160174162405 119 27 2.9574876623777584 119 28 3.3603686491240623 119 29 3.4767040402121694 119 30 3.0575663916621565 119 31 2.0457861182218693 119 32 -0.3757606530538098 119 33 1.1491623225917067 119 34 1.6583102675331745 119 35 2.61618230072159 119 36 4.036013228136113 119 37 3.532048326157849 119 38 3.5819709182930985 119 39 5.057421155111928 119 40 8.62419033054578 119 41 3.6965110592308683 119 42 -2.591954769266117 119 43 -13.382135933432167 119 44 -7.6330810513446 119 45 -2.072186948042205 119 46 -12.891395795846444 119 47 -13.294949799369297 119 48 -9.858653468428429 119 49 -13.867091220197652 119 50 -12.113024793182124 119 51 -7.920197942415637 119 52 -7.653107627136652 119 53 -8.080015678694089 119 54 -11.263595666280455 119 55 -7.563010904765736 119 56 -3.60179657116905 120 1 -0.26035830553747985 120 2 0.6909919607825898 120 3 0.5856106219202175 120 4 0.7215845959882472 120 5 1.171587764560293 120 6 0.9781915261882475 120 7 0.5375209264706058 120 8 0.5751425092437188 120 9 -0.23994185676150437 120 10 0.13601138385464395 120 11 -4.551091699150515 120 12 -15.278715759436935 120 13 -11.575705506481818 120 14 -11.904961020747404 120 15 -13.774544023786664 120 16 -16.870158640980634 120 17 -16.345215229787982 120 18 -16.039558308313907 120 19 -5.929474594670598 120 20 -10.942249135088328 120 21 -6.357701709317229 120 22 -5.771854421177952 120 23 -3.2979136855003794 120 24 -11.100378430980186 120 25 -4.066125948526889 120 26 1.0438160174162405 120 27 2.3780004828905774 120 28 1.5244712132266258 120 29 -0.30278313927500733 120 30 1.590899724995488 120 31 -1.7337010612653074 120 32 -10.452683729976886 120 33 -1.8251966517672642 120 34 -0.6288692196463117 120 35 0.5392592237985099 120 36 -0.06655087442798902 120 37 0.634612428721951 120 38 1.0947914311136095 120 39 1.1753698730606494 120 40 4.490856997212447 120 41 2.0195879823077902 120 42 -3.289390666702012 120 43 -5.5154692667655 120 44 -8.463850282113832 120 45 -5.37475105060631 120 46 -13.070882975333625 120 47 -10.761616466035964 120 48 -8.463781673556635 120 49 -17.826065579172013 120 50 -10.466870947028276 120 51 -8.279172301389998 120 52 -10.98131275534178 120 53 -9.377451576129985 120 54 -13.140518743203533 120 55 -9.398908340663173 120 56 -6.2479504173228975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 221.0 5 3.0 6 6.0 7 19.0 8 45.0 9 55.0 10 39.0 11 35.0 12 20.0 13 27.0 14 36.0 15 38.0 16 50.0 17 76.0 18 94.0 19 96.0 20 135.0 21 155.0 22 209.0 23 238.0 24 255.0 25 290.0 26 339.0 27 378.0 28 455.0 29 555.0 30 647.0 31 789.0 32 866.0 33 1093.0 34 1161.0 35 1388.0 36 1767.0 37 2115.0 38 2019.0 39 1137.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.03874681065685 0.8188453094404557 0.059336616626119984 0.08307126327656797 8 99.27015961549873 0.5636978579481398 0.13054055657746394 0.035601969975671985 9 97.76894321485788 1.2757372574615795 0.6349017978994838 0.3204177297810479 10 38.598469115291046 38.0822405506438 14.056844478727823 9.262445855337328 11 29.680175636385215 38.19498012223343 17.421230641428824 14.703613599952531 12 37.45920607606954 14.329792915207976 18.880911410431377 29.330089598291103 13 22.793899471841435 16.717108776927187 24.360572072874014 36.12841967835737 14 22.95733697264582 21.841808580074765 30.76603572064321 24.434818726636205 15 30.228962236098724 19.399345822182575 25.69134701159679 24.680344930121915 16 30.68519178244864 19.261370383564895 24.44484028025175 25.608597553734715 17 26.855752684981905 22.708123182816113 26.238651872070257 24.19747226013173 18 22.108823354892305 25.384204592654125 29.146146086750136 23.360825965703434 19 29.217887047924464 24.579844408812875 22.156897678009383 24.045370865253282 20 25.574627308903008 29.49456554017937 20.900397933123475 24.030409217794144 21 25.663086690796895 20.84495342075595 27.73393461104848 25.758025277398684 22 24.488221681599715 23.17094879249985 22.39363911469768 29.94719041120275 23 26.855752684981905 23.242152732451196 23.40829525900433 26.493799323562573 24 23.176882454162463 24.749302794754644 30.297276449296863 21.776538301786033 25 30.499020945825666 22.42924108467335 26.392927075298168 20.67881089420281 26 20.989852234288765 21.310307993590886 31.250370897869562 26.449468874250787 27 22.858160660214928 18.37558629697797 31.953927447604347 26.812325595202758 28 22.215622215622215 25.684585684585688 33.762993762993766 18.33679833679834 29 24.836795252225517 20.04747774480712 35.3353115727003 19.780415430267063 30 18.25034126654401 19.407679981007774 43.2725977802837 19.06938097216452 31 17.863501483679524 23.578635014836795 42.64094955489614 15.916913946587538 32 23.36104513064133 16.72209026128266 43.194774346793345 16.72209026128266 33 17.105497771173848 17.277860326894505 45.812778603268946 19.803863298662705 34 15.570461830701651 22.474177846373024 47.95797221892437 13.99738810400095 35 15.037102997922233 17.459186702285546 54.62154942119323 12.882160878598992 36 15.360873694207028 22.97602089268756 47.57834757834758 14.084757834757836 37 15.395127748068923 17.629233511586452 52.875816993464056 14.09982174688057 38 20.818463002617175 18.32619557458958 47.82893171544135 13.02640970735189 39 15.680332739156269 17.18954248366013 47.278669043374926 19.851455733808677 40 12.473118279569892 14.78494623655914 47.53882915173237 25.203106332138592 41 12.987790280105338 13.388795786449606 43.368446253291836 30.25496768015322 42 20.33153110332125 17.194462598776067 47.72146634186917 14.752539956033509 43 12.32542936946574 15.784156415284958 51.637249658287274 20.253164556962027 44 12.32501340322869 17.620777982963006 46.84577351522011 23.208435098588193 45 14.275521731375228 17.955883227302454 53.59414947380938 14.174445567512931 46 13.463252332006418 18.87588378587131 48.11359990493732 19.547263977184954 47 13.755860186339088 22.40816568749629 48.697406682096016 15.1385674440686 48 13.629612025429266 23.427009684510725 49.260293506030536 13.68308478402947 49 11.70193740685544 19.57078986587183 43.81520119225038 24.912071535022353 50 19.037719037719036 18.479358479358478 49.65844965844966 12.824472824472824 51 16.291934332619558 23.51891506067095 47.014037592196054 13.175113014513443 52 14.287410926365796 20.279097387173397 52.01900237529691 13.414489311163896 53 14.261934486653589 20.438737292669877 51.41192556922894 13.887402651447594 54 12.99025433800808 24.851438079391492 43.98621345376753 18.172094128832896 55 14.384254028661472 25.47422251293334 46.678955818516975 13.46256763988821 56 20.478059222261862 20.329408966583422 45.433464145558325 13.759067665596387 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 5.0 9 9.0 10 17.5 11 26.0 12 53.0 13 91.0 14 102.0 15 140.0 16 178.0 17 207.5 18 237.0 19 279.0 20 321.0 21 362.5 22 552.5 23 701.0 24 769.0 25 837.0 26 871.0 27 905.0 28 967.0 29 998.5 30 968.0 31 873.0 32 778.0 33 631.5 34 485.0 35 395.5 36 306.0 37 272.0 38 241.0 39 244.0 40 236.0 41 228.0 42 277.0 43 326.0 44 530.0 45 734.0 46 703.5 47 572.0 48 471.0 49 473.0 50 475.0 51 491.5 52 508.0 53 552.0 54 573.0 55 550.0 56 597.0 57 644.0 58 656.5 59 669.0 60 677.5 61 686.0 62 659.0 63 555.0 64 478.0 65 419.0 66 360.0 67 324.0 68 288.0 69 261.0 70 234.0 71 209.5 72 158.0 73 131.0 74 101.5 75 72.0 76 51.0 77 30.0 78 25.5 79 14.5 80 8.0 81 5.5 82 3.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.011867323325223996 14 0.0 15 0.2254791431792559 16 0.06527027828873197 17 0.0 18 0.0 19 0.08307126327656797 20 0.09493858660179197 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.011867323325223996 27 0.059336616626119984 28 0.10680590992701597 29 0.017800984987835992 30 0.023734646650447992 31 0.017800984987835992 32 0.07713760161395597 33 0.16614252655313594 34 0.041535631638283985 35 0.047469293300895984 36 0.029668308313059992 37 0.13647421824007597 38 0.24328012816709194 39 0.13647421824007597 40 0.6705037678751558 41 0.8603809410787397 42 0.13054055657746394 43 0.15427520322791194 44 0.3916216697323918 45 0.20174449652880794 46 0.13054055657746394 47 0.011867323325223996 48 0.13054055657746394 49 0.4628256096837358 50 0.10680590992701597 51 0.24328012816709194 52 0.07713760161395597 53 0.18987717320358394 54 0.14834154156529994 55 0.21361181985403194 56 0.20767815819141994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 16853.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.13795763365573 #Duplication Level Percentage of deduplicated Percentage of total 1 86.75669272684198 65.18720702545541 2 7.691700229013662 11.558772918768172 3 2.345415778251599 5.28689254138729 4 1.1371712864250179 3.417789117664511 5 0.6870409855484482 2.581142823236219 6 0.4975124378109453 2.242924108467335 7 0.22901366184948274 1.2045333175102357 8 0.22901366184948274 1.3766095057259835 9 0.11845534233593934 0.8010443244526197 >10 0.2763957987838585 3.1804426511600306 >50 0.015794045644791914 0.7832433394647836 >100 0.015794045644791914 2.379398326707411 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 285 1.6910935738444193 No Hit ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 116 0.6883047528629918 No Hit ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 79 0.4687592713463478 No Hit ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 53 0.3144840681184359 No Hit ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.2314128048418679 No Hit ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 28 0.16614252655313594 No Hit ATTTGCGGGGGGCAGTGGTGGCACACGCCTTTAATCCTAGCACTTGGGATAAAGAG 27 0.16020886489052394 No Hit ATTTGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 26 0.15427520322791194 No Hit ATTTGCGGGCTATCGCAGATCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.13647421824007597 No Hit ATTTGCGGGATTCTATCTTTATTTAGTGCATTTGTTGTTCTGATTATTATGTGTCG 22 0.13054055657746394 No Hit ATTTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 20 0.11867323325223997 No Hit ATTTGCGGGAAGCATGGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 19 0.11273957158962795 No Hit ATTTGCGGGTGGCACATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.10680590992701597 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.005933661662611998 0.0 0.0 0.0 0.0 9 0.005933661662611998 0.0 0.0 0.0 0.0 10 0.005933661662611998 0.0 0.0 0.0 0.0 11 0.005933661662611998 0.0 0.0 0.0 0.0 12 0.005933661662611998 0.0 0.0 0.0 0.0 13 0.005933661662611998 0.0 0.0 0.0 0.0 14 0.011867323325223996 0.0 0.0 0.0 0.0 15 0.023734646650447992 0.0 0.0 0.0 0.0 16 0.041535631638283985 0.0 0.0 0.0 0.0 17 0.041535631638283985 0.0 0.0 0.0 0.0 18 0.06527027828873197 0.0 0.0 0.0 0.0 19 0.07713760161395597 0.0 0.0 0.0 0.0 20 0.07713760161395597 0.0 0.0 0.0 0.0 21 0.07713760161395597 0.0 0.0 0.0 0.0 22 0.08307126327656797 0.0 0.0 0.0 0.0 23 0.10087224826440397 0.0 0.0 0.0 0.0 24 0.10680590992701597 0.0 0.0 0.0 0.0 25 0.11273957158962795 0.0 0.0 0.0 0.0 26 0.13054055657746394 0.0 0.0 0.0 0.0 27 0.14240787990268794 0.0 0.0 0.0 0.0 28 0.15427520322791194 0.0 0.0 0.0 0.0 29 0.15427520322791194 0.0 0.0 0.0 0.0 30 0.16614252655313594 0.0 0.0 0.0 0.0 31 0.17800984987835994 0.0 0.0 0.0 0.0 32 0.18987717320358394 0.0 0.0 0.0 0.0 33 0.18987717320358394 0.0 0.0 0.0 0.0 34 0.20174449652880794 0.0 0.0 0.0 0.0 35 0.20174449652880794 0.0 0.0 0.0 0.0 36 0.20174449652880794 0.0 0.0 0.0 0.0 37 0.20767815819141994 0.0 0.0 0.0 0.0 38 0.20767815819141994 0.0 0.0 0.0 0.0 39 0.21361181985403194 0.0 0.0 0.0 0.0 40 0.2195454815166439 0.0 0.0 0.0 0.0 41 0.2254791431792559 0.0 0.0 0.0 0.0 42 0.2314128048418679 0.0 0.0 0.0 0.0 43 0.2432801281670919 0.0 0.0 0.0 0.0 44 0.2432801281670919 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTCTC 55 0.0 50.330334 40 AGCCCCC 15 0.008073837 50.330334 38 ATCCTAG 15 0.008073837 50.330334 34 AAACCAA 15 0.008073837 50.330334 37 TCCTAGC 15 0.008073837 50.330334 35 TATGCTT 60 0.0 50.330334 35 AAGAGCC 15 0.008073837 50.330334 35 AGAGCCC 15 0.008073837 50.330334 36 ATGCTTG 60 0.0 50.330334 36 CTAGCAC 15 0.008073837 50.330334 37 TGGTTGA 75 0.0 50.33033 14 GGTTGAT 80 0.0 50.33033 15 ATATGCT 70 0.0 50.33033 34 ATATGCC 20 3.9353315E-4 50.33033 34 ACATGGT 15 0.008170533 50.17964 11 GTCGCAG 15 0.008170533 50.17964 12 GAAGTGG 30 9.62531E-7 50.17964 9 ACCTGGT 70 0.0 50.17964 11 GCAGTGG 35 4.7451977E-8 50.17964 12 GAACATG 15 0.008170533 50.17964 9 >>END_MODULE