FastQCFastQC Report
Fri 17 Jun 2016
SRR1526251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68433
Sequences flagged as poor quality0
Sequence length56
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1480.21626992825099No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.15489602969327665No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC990.1446670466003244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTGG150.008086137550.4792340
CCTTAGA150.008086137550.4792340
TACCCAC203.964645E-450.4792338
TTCGCGC150.00818149950.32988449
AGATTAA204.023119E-450.32988449
CAGGACT150.00818149950.32988449
CGTTCGC150.00820547150.29268347
CCAACAC150.00820547150.29268343
TTGGGTT204.0378442E-450.29268343
AAGATTA204.0378442E-450.29268348
TCTCAAA204.0378442E-450.29268343
GTTCGCG150.00820547150.29268348
AATACAA252.0051455E-550.2555445
TCGTTCG150.00825356850.21845246
CAAAGAT204.067423E-450.21845246
ATATGCT150.00827769650.18141634
ATATGCC204.0822767E-450.18141634
TGGTTGA355.0744347E-850.14443614
TGCAACG150.00830187650.14443614
GATGCTA301.0128806E-650.14443614
Produced by FastQC (version 0.11.5)