##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526251_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68433 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.65313518331799 39.0 38.0 39.0 33.0 40.0 2 37.35599783730072 39.0 38.0 39.0 33.0 40.0 3 35.95509476422194 39.0 36.0 39.0 28.0 40.0 4 37.3712682477752 39.0 38.0 39.0 33.0 40.0 5 35.64607718498385 39.0 36.0 39.0 24.0 40.0 6 37.260298394049656 39.0 38.0 39.0 33.0 40.0 7 37.57438662633525 39.0 38.0 39.0 34.0 40.0 8 37.398930340625135 39.0 38.0 39.0 33.0 40.0 9 37.279134335773676 39.0 38.0 39.0 33.0 40.0 10 36.82157730919293 39.0 37.0 39.0 32.0 40.0 11 36.273464556573586 38.0 35.0 39.0 31.0 40.0 12 34.403416480353044 38.0 33.0 39.0 23.0 40.0 13 34.02137857466427 38.0 33.0 39.0 21.0 40.0 14 34.11818859322257 38.0 33.0 39.0 22.0 40.0 15 34.07074072450426 38.0 33.0 39.0 22.0 40.0 16 33.34695249367995 38.0 31.0 39.0 20.0 40.0 17 33.3692224511566 37.0 31.0 39.0 20.0 40.0 18 32.74661347595458 36.0 30.0 39.0 18.0 40.0 19 33.631318223664024 37.0 31.0 39.0 23.0 40.0 20 33.827013283065185 37.0 31.0 39.0 23.0 40.0 21 34.608668332529625 38.0 33.0 39.0 25.0 40.0 22 34.284175763155204 38.0 33.0 39.0 23.0 40.0 23 34.89313635234463 38.0 33.0 39.0 26.0 40.0 24 34.46071339850657 38.0 33.0 39.0 24.0 40.0 25 34.7757660777695 38.0 33.0 39.0 26.0 40.0 26 35.5319363464995 38.0 35.0 39.0 28.0 40.0 27 35.417766282349156 38.0 35.0 39.0 28.0 40.0 28 35.38105884587845 38.0 35.0 39.0 28.0 40.0 29 35.19664489354551 38.0 35.0 39.0 27.0 40.0 30 35.16024432656759 38.0 35.0 39.0 27.0 40.0 31 35.52838542808294 38.0 35.0 39.0 29.0 40.0 32 35.18746803442784 38.0 35.0 39.0 27.0 40.0 33 35.323966507386785 38.0 35.0 39.0 28.0 40.0 34 35.34924670845937 38.0 35.0 39.0 29.0 40.0 35 35.148144900851925 38.0 35.0 39.0 28.0 40.0 36 34.42187248841933 38.0 34.0 39.0 27.0 40.0 37 34.161296450542864 38.0 33.0 39.0 27.0 40.0 38 33.82968743150234 37.0 33.0 39.0 25.0 40.0 39 33.29200824163781 36.0 32.0 39.0 22.0 40.0 40 30.197550889190886 35.0 26.0 39.0 9.0 40.0 41 26.72839127321614 32.0 15.0 38.0 5.0 39.0 42 30.972937033302646 35.0 29.0 38.0 10.0 39.0 43 29.598541639267605 35.0 27.0 38.0 5.0 39.0 44 29.449870676428038 35.0 26.0 38.0 4.0 39.0 45 30.538219864685168 35.0 29.0 38.0 4.0 39.0 46 30.207326874461152 35.0 28.0 38.0 4.0 40.0 47 29.68322300644426 35.0 27.0 38.0 4.0 40.0 48 29.744114681513306 35.0 27.0 38.0 4.0 40.0 49 27.234755161983255 34.0 16.0 38.0 4.0 39.0 50 29.29098534332851 35.0 26.0 38.0 4.0 40.0 51 29.389227419519823 35.0 27.0 39.0 4.0 40.0 52 29.183405666856633 35.0 26.0 39.0 4.0 40.0 53 28.80271214180293 35.0 24.0 39.0 4.0 40.0 54 27.729940233513073 35.0 17.0 39.0 4.0 40.0 55 27.99529466777724 35.0 19.0 39.0 4.0 40.0 56 26.63386085660427 33.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.7884919952503253 1 2 1.1532823887713093 1 3 2.5584452173780647 1 4 1.152955383757913 1 5 3.7680845435557586 1 6 1.2301572417853706 1 7 1.205644070617545 1 8 1.2259172619977292 1 9 1.2735626942179366 1 10 1.567918424432861 1 11 -2.2623896956293024 1 12 -12.568386671784776 1 13 -8.890779201141815 1 14 -5.549265742670247 1 15 -6.693378995067416 1 16 -23.542727100712938 1 17 -4.6760508212750445 1 18 -14.326118683717585 1 19 -4.958853607577325 1 20 -5.361063939885174 1 21 -5.807880334509253 1 22 -2.7996958813170707 1 23 -2.870050171243335 1 24 -3.277608911295257 1 25 -3.405131290673893 1 26 2.107248280907413 1 27 1.6963222472312012 1 28 1.914703965766087 1 29 1.6500428770483637 1 30 1.8701758068823153 1 31 1.4739139332358988 1 32 1.8788591956162577 1 33 1.430795547543319 1 34 0.8434126151614336 1 35 0.6363254618641676 1 36 2.2488431623324416 1 37 1.6146200338283379 1 38 1.6801044114142982 1 39 2.704563642436028 1 40 4.326094497276152 1 41 -9.384118494048508 1 42 -8.327754568558412 1 43 -17.118378985825252 1 44 -22.08551088900328 1 45 -10.040224362828056 1 46 -16.327765394226788 1 47 -23.088035224052774 1 48 -13.420855218656957 1 49 -19.603067041540704 1 50 -12.880313150694668 1 51 -8.564050972731192 1 52 -8.686791221886143 1 53 -8.662032302390816 1 54 -7.955215713964122 1 55 -8.20534008774177 1 56 -3.414305613906567 2 1 1.3842366761013878 2 2 1.4848625943653815 2 3 3.4494325470457667 2 4 1.485133246535824 2 5 4.041094345133573 2 6 1.54165616600244 2 7 1.6340447399935911 2 8 1.480638753750064 2 9 1.7753556657694531 2 10 0.9722932750185649 2 11 0.6132976101321077 2 12 -3.183017319645163 2 13 -2.8384244318374883 2 14 1.7771746111428044 2 15 2.124335563861578 2 16 1.0052050054309802 2 17 1.8679367474555306 2 18 -4.289063938319547 2 19 1.5389948265608524 2 20 0.8324574562420111 2 21 1.6100015684312226 2 22 1.576469550191419 2 23 0.89339711539305 2 24 0.7441458101964926 2 25 1.4674481977315565 2 26 0.7722016636470741 2 27 1.2805919101525518 2 28 0.8487226126702225 2 29 1.3704588464483223 2 30 1.7755069089621642 2 31 0.5682242368457509 2 32 0.32889505504729044 2 33 1.0766239004001221 2 34 0.47657063572084013 2 35 0.7667342593779125 2 36 1.5437272168387821 2 37 1.6150981595754033 2 38 1.9237094795472132 2 39 2.1495791815228102 2 40 4.365659402846319 2 41 -4.544649213627757 2 42 -5.602915935998048 2 43 -11.761099521326088 2 44 -12.949842708271747 2 45 -4.898699141694898 2 46 -8.716720689469437 2 47 -17.678400990249283 2 48 -7.817101931542446 2 49 -11.261326663821363 2 50 -7.00534303355386 2 51 -2.797376337302076 2 52 -2.994465140126639 2 53 -2.772718412837861 2 54 -2.1471832014133234 2 55 -1.9859999012727307 2 56 1.4532535541546352 3 1 1.3473155246620934 3 2 1.3634608421751437 3 3 2.893320740621064 3 4 1.6978992039826295 3 5 3.7987080289049544 3 6 1.6882978013799885 3 7 1.4811452823381899 3 8 1.6362499343925307 3 9 1.6209960495825513 3 10 1.6034989487774354 3 11 2.322100280136283 3 12 4.113812050400732 3 13 3.00721595197561 3 14 3.719185458034751 3 15 2.762007653974223 3 16 4.587808259498566 3 17 4.799935078702923 3 18 5.546981118000858 3 19 4.155598915004745 3 20 3.8908637975019147 3 21 2.924770029006943 3 22 3.623194623199346 3 23 3.2651117586971807 3 24 3.4235367154947838 3 25 2.9307356403681837 3 26 2.365234621511071 3 27 2.388435047407455 3 28 2.3722937432501183 3 29 2.85856898412041 3 30 2.013929937748145 3 31 1.380280974434406 3 32 1.653258843115708 3 33 1.250799953800204 3 34 1.6075677154037749 3 35 1.876246149240238 3 36 3.0063888772476233 3 37 3.051059778265433 3 38 3.55595812368572 3 39 4.072816561581213 3 40 7.985600996505063 3 41 8.144962801391017 3 42 5.079603880439162 3 43 -0.268191719907648 3 44 1.1807371832593319 3 45 4.361626265063546 3 46 2.7559534865839623 3 47 -2.349656726586371 3 48 2.6787261869389916 3 49 5.053024608602499 3 50 3.500497600572128 3 51 3.816098840002887 3 52 3.3073704877415295 3 53 3.3511864682635135 3 54 4.2063837572850495 3 55 3.7417848296409133 3 56 4.574238118193016 4 1 1.4109409748421626 4 2 1.4318882131235213 4 3 3.384517219212505 4 4 1.7489196121458974 4 5 3.9251586091370427 4 6 1.8679696701274935 4 7 1.8963113487647618 4 8 1.6432527355129807 4 9 1.664413416529328 4 10 1.576288064423693 4 11 2.676041856766936 4 12 4.354108168848107 4 13 4.326143523004021 4 14 3.9816904600355514 4 15 4.3082261413691825 4 16 4.986967923364112 4 17 4.944793021880194 4 18 6.054183999153317 4 19 4.666003076669405 4 20 4.679179123632366 4 21 3.0850341346491987 4 22 3.325275455532278 4 23 2.8827588175207097 4 24 3.725257403770094 4 25 3.6830365607363333 4 26 2.636943304984463 4 27 2.0561021142341858 4 28 2.2232341194005727 4 29 2.869373305849102 4 30 2.833257668840588 4 31 2.4142945798765822 4 32 2.135451720266566 4 33 2.2744093975777133 4 34 2.44270176902522 4 35 2.3594394265511625 4 36 3.274296040112773 4 37 3.3623843080773597 4 38 3.288451120884602 4 39 4.017794552777694 4 40 6.555629007709541 4 41 10.324434590106499 4 42 6.272681111331526 4 43 7.7352096406365725 4 44 6.046683561810756 4 45 6.012286529169192 4 46 6.304773014395089 4 47 6.053904697983455 4 48 5.915420864810137 4 49 8.560427569786974 4 50 6.242594439307627 4 51 5.526983193744385 4 52 5.26975544172312 4 53 5.067272902837342 4 54 6.256203685256242 4 55 5.326418683182329 4 56 6.230500623195017 5 1 1.6762470972911387 5 2 2.0951535192459687 5 3 3.792680484518627 5 4 2.1468787958193616 5 5 4.649648405055409 5 6 2.0108268129846323 5 7 1.9167195120300633 5 8 1.857538449798696 5 9 1.4501277022436199 5 10 1.9130227583012456 5 11 2.8393071628893836 5 12 4.65002653619505 5 13 4.622061890350963 5 14 4.512302704933511 5 15 4.818430223001833 5 16 5.7930903723437055 5 17 5.506017511676113 5 18 5.931735019561479 5 19 4.829268382791859 5 20 5.015913817509919 5 21 4.013605563220622 5 22 4.243642802471051 5 23 3.821534327724791 5 24 4.266073730300711 5 25 3.5707916627771468 5 26 2.636943304984463 5 27 2.8316123183158197 5 28 3.0599688132781253 5 29 3.410189632379712 5 30 3.3638699137385473 5 31 2.7612333553867856 5 32 2.8395333529196307 5 33 2.519307356761388 5 34 2.636579320045634 5 35 2.4002557530817796 5 36 3.2130715503168545 5 37 3.5868741039957257 5 38 3.6251858147621476 5 39 4.313712920124637 5 40 8.178077987301378 5 41 10.75300601867793 5 42 6.58900764194377 5 43 8.010719844718206 5 44 8.240561112831163 5 45 6.889837549577354 5 46 6.519058728680804 5 47 6.431455718391618 5 48 6.701135150524422 5 49 9.28491736570534 5 50 6.9976964801239525 5 51 5.843309724356629 5 52 5.575877890702714 5 53 5.975436168143464 5 54 6.9194689913786895 5 55 5.775398275019061 5 56 6.710092459929712 6 1 1.6594597371858057 6 2 1.7568827270581266 6 3 3.313308090399687 6 4 2.0473615695740293 6 5 4.141529028272437 6 6 1.7702079848082022 6 7 1.8122648269302175 6 8 1.9136060381243425 6 9 1.877163032504754 6 10 1.8131763681322752 6 11 2.1747252010723983 6 12 3.4227937075797286 6 13 3.650479709091357 6 14 4.207167747724824 6 15 3.374278368711728 6 16 4.375600795867953 6 17 4.331560632150108 6 18 4.9296503147118 6 19 3.518976524112901 6 20 3.066385619133797 6 21 3.1407725590512143 6 22 3.1023217579242015 6 23 2.495662043327158 6 24 3.2625626484486148 6 25 2.5692555644668573 6 26 2.165361123724857 6 27 2.087043523055776 6 28 1.629093237241257 6 29 2.217519015745964 6 30 2.3678198808221538 6 31 1.7673777486279505 6 32 1.7051247507690945 6 33 1.3766696565200007 6 34 1.7811920038288207 6 35 1.6966130056602253 6 36 2.9561042472666017 6 37 2.8322109484109106 6 38 3.074933455754035 6 39 3.5525762073750187 6 40 7.398946980059776 6 41 4.352742687539024 6 42 6.0131902182000765 6 43 6.989872796221388 6 44 6.422040156061353 6 45 5.9580842030774654 6 46 5.549122367039374 6 47 5.5399700918829495 6 48 5.258738837160372 6 49 8.148533780012997 6 50 5.17127558918693 6 51 5.6709375496803105 6 52 5.030014384009718 6 53 4.917283874968131 6 54 6.214290145646846 6 55 5.122556493145023 6 56 5.409785240267652 7 1 1.2293522103040857 7 2 1.4880655227570543 7 3 2.711157552765279 7 4 1.5312325373159652 7 5 3.990991393863837 7 6 1.3723585224426103 7 7 1.3283938591882816 7 8 1.3974770058662784 7 9 1.3825393765907776 7 10 1.4045742175946359 7 11 2.0026821903197103 7 12 2.8529012344614486 7 13 3.5859635800591008 7 14 3.2071677477248244 7 15 3.997934282690224 7 16 4.139041656083002 7 17 4.750915470859788 7 18 5.273736336217176 7 19 3.6802668466935486 7 20 3.8513318556929335 7 21 2.6569015913092784 7 22 3.091569069752161 7 23 2.0117910755852293 7 24 3.1120250140400074 7 25 2.364954489198041 7 26 1.6169740269506647 7 27 1.6676886843461034 7 28 1.371028721112225 7 29 2.217519015745964 7 30 1.9914757948006496 7 31 1.692108931423654 7 32 0.9631892668981266 7 33 0.6347341726490328 7 34 1.447858670495492 7 35 0.8686560164129133 7 36 1.5475020967289623 7 37 1.896727077443174 7 38 1.6125678643561798 7 39 2.423543949310499 7 40 5.001097517694184 7 41 0.5677964509798841 7 42 2.486308497769972 7 43 4.53825989299558 7 44 4.206986392620497 7 45 3.2699121600667134 7 46 3.936219141232918 7 47 4.034593747796933 7 48 4.366265718880797 7 49 5.9227273284001 7 50 3.7304153741331696 7 51 4.520399915271703 7 52 4.169799330246271 7 53 4.583950541634795 7 54 5.332569715539318 7 55 4.337610256585883 7 56 5.571075562848296 8 1 1.283228837355587 8 2 1.7133060434135317 8 3 2.7826912256571887 8 4 1.650530782929998 8 5 3.7217214447976232 8 6 1.5073896486452156 8 7 1.733260865413527 8 8 1.8252076227310212 8 9 1.5177968751759465 8 10 1.517534036496734 8 11 2.126847442158983 8 12 3.634451885282047 8 13 3.7882273038847956 8 14 4.650970803751989 8 15 4.069128397008278 8 16 4.600501757950575 8 17 4.350915470859789 8 18 5.454721056081347 8 19 3.8533285331677973 8 20 4.256085675726887 8 21 3.2377731249821693 8 22 3.651161599463535 8 23 2.9459167122575494 8 24 4.057695642223372 8 25 3.0835736176643636 8 26 2.4083718764130353 8 27 2.0490129627841327 8 28 1.7163597907217323 8 29 2.1833367859779926 8 30 1.6693478944044955 8 31 2.015368693732647 8 32 1.6342701950248966 8 33 1.8881580549353956 8 34 1.3046781385203872 8 35 1.866958223543648 8 36 2.4031897028410185 8 37 2.7920298505048606 8 38 2.786195481786855 8 39 3.2919084088464388 8 40 5.154125248311047 8 41 -1.7599907589805 8 42 3.5728959341027355 8 43 4.872159157285335 8 44 4.980840382433737 8 45 4.241502426054264 8 46 4.949348739421943 8 47 5.5183515293475836 8 48 5.098127631727429 8 49 7.30586258589868 8 50 5.4428092620788355 8 51 6.017853225967805 8 52 4.5710443783504076 8 53 5.888647768573112 8 54 6.341058679885673 8 55 4.602465944191998 8 56 6.727385693012415 9 1 1.2134919952503225 9 2 1.5744902539398495 9 3 3.5840070151308723 9 4 1.910399203982628 9 5 4.325668813218677 9 6 1.7113370170662634 9 7 1.6595766548872106 9 8 2.0646813069415515 9 9 1.9697705593864754 9 10 0.5951656154441025 9 11 1.913031652685305 9 12 3.2890571484399445 9 13 3.3309904617795354 9 14 4.512812909015146 9 15 3.817154712797752 9 16 4.4458964947926845 9 17 5.2009154708597904 9 18 6.058010529765561 9 19 4.613196954220427 9 20 4.239638307305839 9 21 3.498299440771646 9 22 3.9097142310424786 9 23 3.1452588175207126 9 24 3.4563798527496843 9 25 2.899363091348576 9 26 2.995871876413034 9 27 2.6930919101525532 9 28 2.686754527563842 9 29 3.4938631017674666 9 30 3.1029005259834435 9 31 2.509447641101069 9 32 2.4645333529196307 9 33 3.272368581251186 9 34 2.171783401678283 9 35 2.4380108551225916 9 36 3.692663387051546 9 37 3.0361087978732755 9 38 3.19737969231317 9 39 4.020855777267492 9 40 6.819914721995254 9 41 4.2229039778616055 9 42 5.7867117235764205 9 43 6.735974946759018 9 44 6.302550908749527 9 45 5.8079497944753165 9 46 5.447375055211417 9 47 5.950588371452845 9 48 5.592206579095851 9 49 7.321652059582895 9 50 5.256625051552522 9 51 3.7869321733362256 9 52 4.191439115192509 9 53 4.107068821204688 9 54 5.285795521990931 9 55 3.8432554178762075 9 56 3.795806745643997 10 1 1.4908729476312743 10 2 1.8417521587017518 10 3 3.109007015130871 10 4 2.019327775411199 10 5 4.105430717980582 10 6 1.7982417789710254 10 7 1.904814750125304 10 8 1.7503955926558348 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2.66090155288704 115 26 2.3622180302591858 115 27 2.634438063998708 115 28 2.3050237583330713 115 29 2.516940024844388 115 30 2.675015910598823 115 31 2.062332256485689 115 32 2.3395333529196307 115 33 2.218522427405034 115 34 2.085244940139823 115 35 1.9649339320456676 115 36 3.1618941562823153 115 37 3.3928395671040477 115 38 3.890648923082402 115 39 3.8862403926521054 115 40 7.700683952764486 115 41 6.844057824015451 115 42 2.3453655697302658 115 43 -4.216909668625597 115 44 -6.47341062971201 115 45 3.18968056370608 115 46 -3.0401249447885874 115 47 -2.8330654747010016 115 48 -2.239524190134915 115 49 -14.686040248109416 115 50 -2.0770287946013255 115 51 -0.9351832112791634 115 52 -0.34798396173056645 115 53 -1.3429311787953075 115 54 -6.641127554932144 115 55 -1.6355907359699486 115 56 1.249652899490151 116 1 1.041656552212352 116 2 1.389521899509468 116 3 2.6659690404473295 116 4 1.1978992039826295 116 5 3.789276408155388 116 6 1.0964635993447445 116 7 1.1355260219758136 116 8 1.126073712004839 116 9 1.1680300530573575 116 10 1.4790263749377672 116 11 -1.4602278409855813 116 12 -13.928506142699295 116 13 -6.622965234422999 116 14 -3.7691491163013104 116 15 -7.753256679607311 116 16 -4.555527555840229 116 17 -3.236426301292113 116 18 -8.895312255044566 116 19 -3.472720767298558 116 20 -4.01146928763087 116 21 1.7926032382399981 116 22 1.900062332308302 116 23 1.1232651466346368 116 24 1.746569726167408 116 25 1.2272111926143978 116 26 0.9425490916029062 116 27 1.521098239266479 116 28 1.6919760465511828 116 29 1.9482934815143054 116 30 1.9562233107935683 116 31 1.2874539702149974 116 32 1.7445966440588734 116 33 0.9571787078334637 116 34 1.159441629526384 116 35 0.7563652855023406 116 36 1.7116507288236988 116 37 1.5122163928099894 116 38 2.1257024771232977 116 39 3.177184891191544 116 40 6.4218134561724725 116 41 3.77005587659578 116 42 -0.4704085295881377 116 43 -8.58886682539288 116 44 -7.8007718760605975 116 45 0.5343738451082238 116 46 -1.997086970105041 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1.7049742128785823 117 30 1.153363488946404 117 31 1.2506513448047727 117 32 1.7469407603270355 117 33 1.3019982108808108 117 34 1.2764130313079107 117 35 1.4917145588262954 117 36 1.8222930166811722 117 37 2.6273125015769807 117 38 2.563583396016874 117 39 3.4486335550452694 117 40 6.658803610884142 117 41 3.9053113852690124 117 42 -2.51189938753469 117 43 -8.397821349537278 117 44 -10.757171313472693 117 45 0.08711646114198146 117 46 -3.440124944788586 117 47 -3.801726443361968 117 48 -3.7221452727559985 117 49 -9.804273866343035 117 50 -4.708652726225257 117 51 -4.7209381970341475 117 52 -4.919209032955639 117 53 -5.55404228990642 117 54 -8.949389663194253 117 55 -4.285448285827496 117 56 -3.0967858469485954 118 1 0.371825328583661 118 2 0.7369902539398439 118 3 2.342340348464205 118 4 0.8645658706492938 118 5 2.8506688132186753 118 6 1.169670350399599 118 7 0.8595766548872064 118 8 1.0646813069415515 118 9 0.9739372260531383 118 10 1.1701656154440983 118 11 -2.0661350139813663 118 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119 33 0.9350804456579667 119 34 -0.12440303899968086 119 35 0.27106170258021933 119 36 1.311307454848155 119 37 1.6947952385512437 119 38 0.6395407092623273 119 39 2.3488218789624042 119 40 5.134321501656274 119 41 1.0148531304039778 119 42 -3.9007882764235795 119 43 -11.313389460020641 119 44 -7.670542311589454 119 45 -5.9863298665416345 119 46 -16.728260538008925 119 47 -16.275047221767494 119 48 -11.734700200565165 119 49 -14.141059804823888 119 50 -10.142739355227139 119 51 -9.062432233443438 119 52 -8.694789698366812 119 53 -8.875134568625818 119 54 -13.069289223771777 119 55 -9.912253056700063 119 56 -6.334701728932274 120 1 0.02762243003293463 120 2 0.03481634089636998 120 3 2.1307461455656522 120 4 0.15442094311306676 120 5 2.765886204523028 120 6 0.1559022344575709 120 7 0.34218535053938126 120 8 0.47555087215894076 120 9 0.5681401246038646 120 10 -0.05592134107764224 120 11 -4.012511825575569 120 12 -11.675616764603532 120 13 -12.610857364307424 120 14 -11.690447960550074 120 15 -11.772519200245725 120 16 -16.919864374772537 120 17 -13.466475833488037 120 18 -15.259924252843135 120 19 -8.121042176214353 120 20 -14.031557344868077 120 21 -5.382135341837049 120 22 -3.045177073305343 120 23 -3.182458573783638 120 24 -9.27296797333727 120 25 -3.1528108216948993 120 26 0.034458832934774364 120 27 -0.26288635071701094 120 28 -0.2909628637405106 120 29 0.05038484089790529 120 30 -0.13568643053829987 120 31 -1.2139219241163204 120 32 -11.964814473167326 120 33 -1.2841531578792527 120 34 -1.3097383374521527 120 35 -1.320684797051321 120 36 -0.6551626999049773 120 37 -1.1850868543006357 120 38 -0.5749029163824773 120 39 -0.22044857055859524 120 40 3.0742625480822134 120 41 -0.5890525438775249 120 42 -7.922527406858361 120 43 -13.710220705414892 120 44 -12.187123004293948 120 45 -10.1882458576986 120 46 -17.0488205969625 120 47 -15.726042063329764 120 48 -13.196380377425886 120 49 -18.291391418677975 120 50 -12.996092339751826 120 51 -10.959263478837691 120 52 -10.901495667416185 120 53 -11.638583352708352 120 54 -14.068552304096023 120 55 -9.668701103862922 120 56 -7.830280210877742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 310.0 5 26.0 6 33.0 7 75.0 8 106.0 9 132.0 10 84.0 11 81.0 12 72.0 13 84.0 14 99.0 15 162.0 16 243.0 17 279.0 18 341.0 19 425.0 20 486.0 21 668.0 22 794.0 23 1012.0 24 1141.0 25 1311.0 26 1387.0 27 1590.0 28 1888.0 29 2176.0 30 2665.0 31 3220.0 32 3613.0 33 4058.0 34 4726.0 35 5429.0 36 6903.0 37 8868.0 38 8706.0 39 5235.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.36141919833999 0.48222348866774806 0.10375111422851549 0.05260619876375433 8 98.98586939049873 0.7379480659915538 0.1505121797962971 0.1256703637134131 9 94.16947963701723 3.489544517995704 1.1485686730086362 1.1924071719784315 10 30.366928236377188 48.624201773997925 10.497859219966974 10.511010769657913 11 26.893457834670407 36.044013852965676 21.675215173965775 15.387313138398143 12 39.88280507942075 17.080940481931233 19.471599959084067 23.564654479563956 13 24.77015946329129 19.63108584123829 24.020345820483215 31.57840887498721 14 25.5256966668128 24.46918884164073 25.79018894393056 24.214925547615916 15 28.00333948501626 21.516243372293992 25.318569294314088 25.16184784837566 16 27.372406836157364 22.141489159515213 28.34461484481221 22.141489159515213 17 29.725424868119184 24.806745283708153 21.6489120745839 23.818917773588765 18 26.57635935878889 25.969926789706722 22.828167696871393 24.625546154633 19 23.851802012637492 30.808272876199393 22.02931195881114 23.310613152351976 20 25.23220946390697 28.185475023769474 21.551963724127845 25.030351788195716 21 25.492087150935948 24.977715429690353 24.947028480411497 24.583168938962196 22 25.90563032455102 26.25049318311341 23.19494980491868 24.64892668741689 23 25.41317785279032 28.619233410781348 23.428755132757587 22.538833603670742 24 23.24171087048646 30.153580874724184 24.580246372364208 22.024461882425143 25 24.463701192424598 27.907996259060084 26.500759878419455 21.12754267009586 26 23.371669906033993 26.735740694734687 30.18749360651186 19.705095792719458 27 22.773682054882688 23.762506582411795 32.37083845298695 21.092972909718565 28 20.246942477617182 27.09052606940137 34.22669553513956 18.435835917841885 29 19.75236452409841 26.40226877366351 35.82820471589165 18.017161986346427 30 17.628317549397874 25.67666315912545 39.990354261662574 16.7046650298141 31 16.91833184721755 25.487129262837847 42.53994240692286 15.054596483021736 32 18.567437506399287 22.958444863750056 43.10266649114339 15.37145113870727 33 16.11775205934423 24.849664213498766 45.30557303173512 13.72701069542189 34 14.289473684210527 24.58625730994152 47.95029239766082 13.173976608187136 35 15.331764293127897 25.21671758738141 48.20851667227038 11.243001447220314 36 15.496828134592336 24.790247610138273 47.28125822200134 12.431666033268044 37 15.250168340310918 24.49131949527183 48.464443598676695 11.79406856574055 38 14.851934883584628 24.493384324585694 49.14941316101807 11.50526763081161 39 13.93183415319747 25.663211524947293 47.41889201218084 12.986062309674395 40 11.312729948491537 19.025754231052243 42.6122148638705 27.049300956585725 41 10.543846720707444 17.671333824613118 39.665438467207075 32.11938098747237 42 12.723681322701003 24.84161240763772 48.28004974760407 14.154656522057211 43 11.510180166984034 22.526732093159513 46.891753332356814 19.071334407499634 44 11.599013099923633 21.967338306996417 45.78511425718146 20.648534335898493 45 13.406937753503287 26.329199185860926 47.46752961504107 12.796333445594717 46 14.31018830351129 25.069552230064136 47.66890209974522 12.951357366679355 47 13.097500365443649 24.240608098231252 48.525069434293236 14.136822102031868 48 13.265709392701883 26.02204364817986 48.71265680118269 11.999590157935568 49 11.061491905867145 21.469283426859008 42.25548667626406 25.213737991009783 50 12.60809130148511 26.396956617162925 48.9150632818787 12.079888799473261 51 14.729113849851258 26.817509049077508 46.63901874294758 11.814358358123654 52 12.7734289267686 28.388323944692367 46.202355695369086 12.635891433169947 53 13.610013175230568 27.849509588640025 46.32557458644415 12.214902649685259 54 12.233894480828681 26.1424406253205 44.56214378855142 17.061521105299402 55 13.482635888272085 27.653684472631934 45.46892622266489 13.394753416431094 56 15.115717359799746 28.35624258925827 43.95649437149591 12.571545679446078 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 4.5 7 9.0 8 12.0 9 15.0 10 68.5 11 122.0 12 208.5 13 380.5 14 466.0 15 471.0 16 476.0 17 629.0 18 782.0 19 988.5 20 1195.0 21 1300.5 22 1634.0 23 1862.0 24 2358.0 25 2854.0 26 3438.5 27 4023.0 28 4275.0 29 4712.0 30 4897.0 31 4730.0 32 4563.0 33 4064.0 34 3565.0 35 3041.5 36 2518.0 37 2112.5 38 1572.5 39 1438.0 40 1374.5 41 1311.0 42 1281.0 43 1251.0 44 1241.0 45 1231.0 46 1335.5 47 1539.5 48 1639.0 49 1807.0 50 1975.0 51 1907.0 52 1839.0 53 1943.5 54 2125.5 55 2203.0 56 2257.5 57 2312.0 58 2317.0 59 2322.0 60 2401.5 61 2481.0 62 2446.0 63 2174.0 64 1937.0 65 1752.0 66 1567.0 67 1387.5 68 1208.0 69 1017.5 70 827.0 71 717.0 72 498.5 73 390.0 74 356.5 75 323.0 76 266.0 77 209.0 78 152.5 79 76.0 80 56.0 81 37.5 82 19.0 83 14.0 84 9.0 85 5.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.023380532783890812 14 0.0 15 0.23234404453991495 16 0.046761065567781625 17 0.0 18 0.0 19 0.09498341443455642 20 0.09936726433153595 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0014612832989931758 26 0.005845133195972703 27 0.10082854763052912 28 0.11251881402247453 29 0.03799336577382257 30 0.013151549690938583 31 0.03214823257784987 32 0.0964446977335496 33 0.1256703637134131 34 0.0482223488667748 35 0.03799336577382257 36 0.027764382680870343 37 0.17389271258018793 38 0.27179869361273074 39 0.1885055455701197 40 0.7057998334137039 41 0.8519281633130215 42 0.12859293031139948 43 0.23818917773588766 44 0.4982976049566729 45 0.2045796618590446 46 0.20311837856005147 47 0.03360951587684304 48 0.16658629608522205 49 0.5246007043385501 50 0.12859293031139948 51 0.2834889600046761 52 0.12859293031139948 53 0.17973784577616062 54 0.26303099381877165 55 0.23380532783890814 56 0.1753539958791811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 68433.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.06223605570412 #Duplication Level Percentage of deduplicated Percentage of total 1 87.77874696601941 67.6442651936931 2 7.118476941747573 10.971315008840763 3 2.2963440533980584 5.308842225242208 4 1.0467233009708738 3.226513524176932 5 0.5214654126213593 2.0092645361156167 6 0.29960558252427183 1.3852965674455306 7 0.21806735436893204 1.1763330556895066 8 0.1573877427184466 0.9702921105314687 9 0.10239684466019418 0.7101836833106835 >10 0.4361347087378641 5.184633144827788 >50 0.020858616504854367 1.0418949921821343 >100 0.0037924757281553394 0.37116595794426666 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 148 0.21626992825099 No Hit GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 106 0.15489602969327665 No Hit GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 99 0.1446670466003244 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.018996682886911285 0.0 0.0 0.0 0.0 11 0.023380532783890812 0.0 0.0 0.0 0.0 12 0.02776438268087034 0.0 0.0 0.0 0.0 13 0.030686949278856693 0.0 0.0 0.0 0.0 14 0.03507079917583622 0.0 0.0 0.0 0.0 15 0.043838498969795275 0.0 0.0 0.0 0.0 16 0.049683632165767974 0.0 0.0 0.0 0.0 17 0.0540674820627475 0.0 0.0 0.0 0.0 18 0.06283518185670656 0.0 0.0 0.0 0.0 19 0.08037058144462467 0.0 0.0 0.0 0.0 20 0.09498341443455642 0.0 0.0 0.0 0.0 21 0.1022898309295223 0.0 0.0 0.0 0.0 22 0.11836394721844724 0.0 0.0 0.0 0.0 23 0.13736063010535854 0.0 0.0 0.0 0.0 24 0.1622024461882425 0.0 0.0 0.0 0.0 25 0.18266041237414699 0.0 0.0 0.0 0.0 26 0.19435067876609238 0.0 0.0 0.0 0.0 27 0.19727324536407873 0.0 0.0 0.0 0.0 28 0.21042479505501732 0.0 0.0 0.0 0.0 29 0.21919249484897638 0.0 0.0 0.0 0.0 30 0.22649891134394226 0.0 0.0 0.0 0.0 31 0.241111744333874 0.0 0.0 0.0 0.0 32 0.24549559423085354 0.0 0.0 0.0 0.0 33 0.2528020107258194 0.0 0.0 0.0 0.0 34 0.25718586062279897 0.0 0.0 0.0 0.0 35 0.26303099381877165 0.0 0.0 0.0 0.0 36 0.2674148437157512 0.0 0.0 0.0 0.0 37 0.2732599769117239 0.0 0.0 0.0 0.0 38 0.2747212602107171 0.0 0.0 0.0 0.0 39 0.2761825435097102 0.0 0.0 0.0 0.0 40 0.2776438268087034 0.0 0.0 0.0 0.0 41 0.2820276767056829 0.0 0.0 0.0 0.0 42 0.29371794309762833 0.0 0.0 0.0 0.0 43 0.3010243595925942 0.0 0.0 0.0 0.0 44 0.3097920593865533 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTTGG 15 0.0080861375 50.47923 40 CCTTAGA 15 0.0080861375 50.47923 40 TACCCAC 20 3.964645E-4 50.47923 38 TTCGCGC 15 0.008181499 50.329884 49 AGATTAA 20 4.023119E-4 50.329884 49 CAGGACT 15 0.008181499 50.329884 49 CGTTCGC 15 0.008205471 50.292683 47 CCAACAC 15 0.008205471 50.292683 43 TTGGGTT 20 4.0378442E-4 50.292683 43 AAGATTA 20 4.0378442E-4 50.292683 48 TCTCAAA 20 4.0378442E-4 50.292683 43 GTTCGCG 15 0.008205471 50.292683 48 AATACAA 25 2.0051455E-5 50.25554 45 TCGTTCG 15 0.008253568 50.218452 46 CAAAGAT 20 4.067423E-4 50.218452 46 ATATGCT 15 0.008277696 50.181416 34 ATATGCC 20 4.0822767E-4 50.181416 34 TGGTTGA 35 5.0744347E-8 50.144436 14 TGCAACG 15 0.008301876 50.144436 14 GATGCTA 30 1.0128806E-6 50.144436 14 >>END_MODULE