FastQCFastQC Report
Fri 17 Jun 2016
SRR1526250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282140
Sequences flagged as poor quality0
Sequence length56
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG91723.2508683632239315No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG26970.9559084142624229No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG25880.9172751116467003No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG21550.7638052030906642No Hit
CGCACCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC6330.22435670234635285No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG6320.2240022683774013No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG4740.16800170128305097No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4350.15417877649393918No Hit
CGCACCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC3860.13681151201531155No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC3850.13645707804635995No Hit
CGCACCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC3300.11696320975402281No Hit
CGCACCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA3220.11412773800241016No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG2950.10455802084071739No Hit
CGCACCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC2860.10136811512015312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT150.00820217750.3392535
CCAACGT204.0880087E-450.22218348
CAACGAT4400.050.19524446
AAGCGTA150.00834849950.114643
CTTGAGG204.1716974E-450.01638833
TCGTTGA150.00845504749.9540914
TGGTCGA204.1974362E-449.9540914
CGTATCG150.00846694949.9363213
CACGGGA150.00849078849.90081828
AAGCCGT252.1080312E-549.90081810
TTACGTA150.00849078849.90081810
ACGGGAG150.00849675549.8919529
CGTCTCA4450.049.88993541
GCGTAAG150.0085266449.84765625
GGAACGA204.267935E-449.7857788
CGGGATA1700.049.7593046
CCGGTAT150.00858664349.7593045
CGCACCG282350.049.2834781
CACCGGG269450.049.2237633
GCACCGG280250.049.2177662
Produced by FastQC (version 0.11.5)