##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526250_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 282140 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.26521939462678 39.0 38.0 40.0 35.0 40.0 2 37.55413270007798 39.0 38.0 40.0 33.0 40.0 3 37.81448926065074 39.0 38.0 40.0 35.0 40.0 4 36.6109555539803 39.0 38.0 40.0 31.0 40.0 5 37.86740625221521 39.0 38.0 40.0 35.0 40.0 6 37.873233146664774 39.0 38.0 40.0 35.0 40.0 7 37.611551003048135 39.0 38.0 40.0 34.0 40.0 8 37.77642659672503 39.0 38.0 40.0 35.0 40.0 9 37.78974268093854 39.0 38.0 40.0 35.0 40.0 10 37.2864110016304 39.0 38.0 40.0 33.0 40.0 11 36.66675054937265 38.0 36.0 40.0 32.0 40.0 12 34.90196710852768 38.0 35.0 39.0 24.0 40.0 13 34.639281916778906 38.0 34.0 39.0 23.0 40.0 14 34.513727227617494 38.0 33.0 39.0 23.0 40.0 15 34.43427376479762 38.0 33.0 39.0 23.0 40.0 16 33.85202381796272 38.0 32.0 39.0 22.0 40.0 17 33.81667257389948 38.0 32.0 39.0 22.0 40.0 18 33.415247749344296 38.0 31.0 39.0 19.0 40.0 19 34.211458850216204 38.0 32.0 39.0 24.0 40.0 20 34.25166229531438 38.0 32.0 39.0 24.0 40.0 21 35.0818458921103 38.0 34.0 39.0 26.0 40.0 22 34.82621039200397 38.0 33.0 39.0 25.0 40.0 23 35.33038562415822 38.0 35.0 39.0 27.0 40.0 24 34.79393209045155 38.0 33.0 39.0 25.0 40.0 25 35.08838519883746 38.0 34.0 39.0 27.0 40.0 26 35.60152406606649 38.0 35.0 39.0 28.0 40.0 27 35.52416176366343 38.0 35.0 39.0 28.0 40.0 28 35.29794073864039 38.0 35.0 39.0 28.0 40.0 29 35.149287587722405 38.0 35.0 39.0 27.0 40.0 30 34.79454171687814 38.0 34.0 39.0 26.0 40.0 31 35.22794711845183 38.0 35.0 39.0 28.0 40.0 32 34.870521726802295 38.0 34.0 39.0 27.0 40.0 33 35.03386971007301 38.0 35.0 39.0 27.0 40.0 34 34.978663075069115 38.0 34.0 39.0 27.0 40.0 35 34.76621180973984 38.0 34.0 39.0 27.0 40.0 36 34.36912525696463 37.0 33.0 39.0 27.0 40.0 37 34.055678032182605 37.0 33.0 39.0 27.0 40.0 38 33.847313390515346 36.0 33.0 39.0 26.0 40.0 39 33.455766640674845 36.0 32.0 38.0 23.0 40.0 40 30.206868930318283 35.0 26.0 38.0 10.0 40.0 41 27.084213511022895 32.0 17.0 38.0 7.0 39.0 42 31.03015878641809 35.0 29.0 38.0 15.0 39.0 43 29.48235627702559 34.0 27.0 38.0 7.0 39.0 44 29.673516693839936 34.0 27.0 38.0 6.0 39.0 45 30.829602325086835 35.0 29.0 38.0 10.0 39.0 46 30.51385127950663 35.0 28.0 38.0 4.0 40.0 47 30.085886439356347 35.0 28.0 38.0 4.0 40.0 48 30.115914085205926 35.0 28.0 38.0 4.0 40.0 49 27.759388955837526 33.0 18.0 38.0 4.0 39.0 50 29.771390090026227 35.0 27.0 38.0 4.0 40.0 51 30.154008648188842 35.0 28.0 39.0 4.0 40.0 52 29.930779045863755 35.0 28.0 39.0 4.0 40.0 53 29.479127383568443 35.0 27.0 39.0 4.0 40.0 54 28.53726518749557 34.0 23.0 38.0 4.0 40.0 55 28.975295952364075 35.0 26.0 38.0 4.0 40.0 56 27.947412632026655 34.0 22.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.37555219673921414 1 2 1.0259884244201203 1 3 0.7503146469051956 1 4 2.4192413609181287 1 5 0.7250285546363102 1 6 0.752608123931708 1 7 0.6554652875377087 1 8 0.4772968151914938 1 9 0.5655874310646283 1 10 0.8738320296915276 1 11 -2.8031513907907524 1 12 -13.61134873158231 1 13 -8.630136511948788 1 14 -5.350831632820292 1 15 -6.528981670682118 1 16 -23.15781056800932 1 17 -4.54133322200839 1 18 -12.892015487896295 1 19 -5.649884100653804 1 20 -5.631812338431487 1 21 -5.273491469298644 1 22 -2.330980955376628 1 23 -2.874036668809765 1 24 -3.3906729937846087 1 25 -2.5931467878610768 1 26 2.2567888679116237 1 27 2.1160230697952827 1 28 2.1450509292067395 1 29 2.3239394353715284 1 30 2.4296137901850585 1 31 1.7347231840865334 1 32 1.9044190744354026 1 33 2.1462932099426055 1 34 1.675086513662336 1 35 1.3410577611568684 1 36 1.6807881851920996 1 37 1.6783190521484386 1 38 1.8736726214712505 1 39 2.4708080136748407 1 40 5.165082466787371 1 41 -10.315079421246008 1 42 -9.585826307057236 1 43 -18.16843036828419 1 44 -22.343152158663408 1 45 -12.070486325043841 1 46 -18.610659099344197 1 47 -23.733247064264283 1 48 -15.591271508538036 1 49 -20.70055550679222 1 50 -14.712213240638008 1 51 -11.592288961457644 1 52 -11.505787124590043 1 53 -11.145646663031421 1 54 -10.571578318478409 1 55 -10.923106761647919 1 56 -6.360997714829509 2 1 0.995643016572302 2 2 1.6115308888285682 2 3 1.402499232079471 2 4 2.988736942655976 2 5 1.4144615443270325 2 6 1.4132610448939076 2 7 1.2554284686864605 2 8 1.0836112481811853 2 9 1.146282080058242 2 10 1.4890750341097885 2 11 0.9516964246240747 2 12 -2.3459154964325784 2 13 -1.5490429920666031 2 14 2.838018391725612 2 15 2.343103503593781 2 16 2.151774606266578 2 17 2.951057548732898 2 18 -1.913892022015098 2 19 2.0944089774021606 2 20 2.4076145147840293 2 21 2.3508028360523596 2 22 2.514686693126073 2 23 2.0927036355594026 2 24 1.502429608080881 2 25 1.9386094713436393 2 26 2.3114341796445643 2 27 2.306591307399607 2 28 2.2618280524271626 2 29 2.511930353388223 2 30 2.6076943007397944 2 31 1.644026080502833 2 32 2.014722216310709 2 33 1.5946854534379469 2 34 1.7457787080658704 2 35 1.430036651682144 2 36 1.6881519554424642 2 37 1.6074734458646844 2 38 1.9033424791050209 2 39 1.737345814362122 2 40 4.029711823684774 2 41 -7.600241424191516 2 42 -7.604849380204019 2 43 -15.106022415412319 2 44 -15.481069438977597 2 45 -7.71472417482293 2 46 -11.521250655554308 2 47 -18.075877157538706 2 48 -8.958355455518888 2 49 -13.679384667322413 2 50 -7.816349224928629 2 51 -2.9118765903236223 2 52 -2.6176857500195965 2 53 -2.4536977185051576 2 54 -1.545866487354207 2 55 -2.10784534780403 2 56 0.7443655645028429 3 1 1.2494093395963546 3 2 1.7723693768010733 3 3 1.6577432183337706 3 4 3.193836599013366 3 5 1.6000285546363102 3 6 1.6819414572650402 3 7 1.261036716109139 3 8 1.38246348185816 3 9 1.307539812017005 3 10 1.632593934453432 3 11 1.9112771806378248 3 12 3.825960792227214 3 13 2.8094587261464525 3 14 3.3610493195606637 3 15 3.4550897578893114 3 16 4.86709419389544 3 17 4.769428682753514 3 18 5.191008321627514 3 19 4.176353994584289 3 20 3.9001162329970853 3 21 3.4236275783204064 3 22 3.445257139861468 3 23 3.027487140714044 3 24 3.581707958596347 3 25 3.418829402615117 3 26 2.7591936298163873 3 27 2.759237355509569 3 28 2.5876699768257865 3 29 2.4811537210858177 3 30 2.9491375997088696 3 31 2.672342231705578 3 32 2.8000143125306423 3 33 2.5475789242283255 3 34 2.069276989852817 3 35 2.302772046871148 3 36 3.307169137573048 3 37 3.2986761950055765 3 38 3.8380297643283896 3 39 4.303641347008174 3 40 7.376034847739756 3 41 8.187634864468276 3 42 4.61141178818086 3 43 -1.16800179685562 3 44 2.115157365146114 3 45 3.944251770194249 3 46 1.9993885197034231 3 47 -3.3137946833119045 3 48 1.6965380152714893 3 49 3.555206397969684 3 50 2.1076439022191344 3 51 3.2037110385423553 3 52 3.692046208743289 3 53 3.6973057179209583 3 54 4.699707395807305 3 55 3.409536095494939 3 56 3.828097523265729 4 1 1.120762022094162 4 2 1.5493896054730172 4 3 1.5864749685536452 4 4 3.187074945539308 4 5 1.4997647023935627 4 6 1.4592923807478968 4 7 1.2736805155814324 4 8 1.2178970790437376 4 9 1.2628326880064549 4 10 1.3219096776372439 4 11 2.0790731349034317 4 12 4.129644169535922 4 13 4.015995225706696 4 14 4.2310018261569695 4 15 3.9454890542833283 4 16 4.562207650359824 4 17 4.730842930774621 4 18 5.083680265339034 4 19 4.028357512614193 4 20 3.609196268177385 4 21 3.273632855365257 4 22 3.2564515110136227 4 23 3.050262866307712 4 24 3.489148591841726 4 25 3.107828523107635 4 26 2.349223533070564 4 27 2.576264620241318 4 28 2.5645917006604364 4 29 2.4589830966355066 4 30 2.4113011880993724 4 31 1.8326940346959049 4 32 2.4669976018886004 4 33 2.041207772073527 4 34 1.84498851140075 4 35 1.6535970248834673 4 36 2.424945742674197 4 37 2.8426691589457747 4 38 2.607943572595765 4 39 3.430515577439131 4 40 6.374311013087162 4 41 9.194579455497301 4 42 5.4828066870375025 4 43 7.124304271745967 4 44 6.15608612504057 4 45 5.01656135682574 4 46 5.306871369307643 4 47 4.354004788983609 4 48 5.095686652034903 4 49 7.5466646213645845 4 50 5.504632468621949 4 51 4.398476210046315 4 52 4.857724309007143 4 53 4.7918140732419765 4 54 5.4427293834942 4 55 3.810190449056943 4 56 3.803548710600822 5 1 1.3358420229761165 5 2 1.7669023387323648 5 3 1.7330709806364624 5 4 3.303169932346698 5 5 1.7532318972546932 5 6 1.7001475853987458 5 7 1.302351479340338 5 8 1.4395182636594583 5 9 1.4044088927969511 5 10 1.6743692362175935 5 11 2.1163505325412544 5 12 4.276144636238357 5 13 4.304975903490927 5 14 4.328046534045342 5 15 4.512345096793673 5 16 5.40249252258625 5 17 5.180281050441536 5 18 5.6163825091855415 5 19 4.397419918446872 5 20 4.396034524547687 5 21 3.2854437343092613 5 22 3.9584307703164328 5 23 3.4633422939173855 5 24 4.125774810964032 5 25 3.4480141751313624 5 26 2.8636913085536193 5 27 3.0054397696228463 5 28 2.983176012108977 5 29 3.558063656090461 5 30 2.8860735328565 5 31 2.663725704314679 5 32 3.1171545168017616 5 33 2.613480502687004 5 34 2.0080550771322976 5 35 2.548313365348399 5 36 3.149650103218363 5 37 3.6755749879489414 5 38 3.7845775823413916 5 39 4.327760195661838 5 40 7.731503742818678 5 41 9.89089948842371 5 42 6.71159377518179 5 43 7.8511179803031546 5 44 7.566277142304887 5 45 6.232214629989983 5 46 6.246898269007048 5 47 5.877336235908153 5 48 6.350829565875014 5 49 8.365650223410725 5 50 6.701419203983299 5 51 5.916067584503356 5 52 6.170686877266963 5 53 6.977877677066733 5 54 6.882377776494398 5 55 6.213458101065967 5 56 6.0821810887253385 6 1 1.0821754537768342 6 2 1.5165830047937092 6 3 1.4810434024958283 6 4 3.1484737997500147 6 5 1.6304152949678041 6 6 1.4395326174860372 6 7 1.3968820199765446 6 8 1.2027802406058612 6 9 1.4030315247241916 6 10 1.3278572862029705 6 11 2.207733902553109 6 12 3.8858723944371576 6 13 3.737908081579235 6 14 4.385734402433592 6 15 4.467384417189493 6 16 4.910033420414777 6 17 4.784699400985559 6 18 5.168083828073186 6 19 3.8746818030557435 6 20 3.994289345335943 6 21 2.963008793790017 6 22 3.332166900450787 6 23 2.932724709774817 6 24 3.227453814949932 6 25 3.157297174254154 6 26 2.4739192283430853 6 27 2.4957751087324027 6 28 2.0474121499381255 6 29 2.542089264363902 6 30 2.616209422913286 6 31 2.047996007027862 6 32 2.122371586932111 6 33 2.173884633252264 6 34 2.076230949337159 6 35 1.635980150001906 6 36 2.4540715316798654 6 37 2.4704625670129445 6 38 2.563606191584377 6 39 2.9824332438774164 6 40 6.084248475732391 6 41 2.7807177373964542 6 42 4.412266300151394 6 43 5.934075551210679 6 44 4.9917761496765 6 45 4.412988418444712 6 46 4.977311171637126 6 47 5.022360012820691 6 48 4.763108917665594 6 49 6.947530523199887 6 50 5.448829905902379 6 51 4.612130928045115 6 52 4.628996484986384 6 53 4.494513821051715 6 54 4.631427469472133 6 55 4.179547145218699 6 56 3.926130672437001 7 1 1.0975399832082715 7 2 1.3935951212775421 7 3 1.6003215084394355 7 4 3.000576272404338 7 5 1.5222187563654188 7 6 1.6176417454494825 7 7 1.1009675518440076 7 8 1.1334567575546046 7 9 1.2257184863685922 7 10 1.5324363936273002 7 11 1.8877536455753798 7 12 3.835551570325201 7 13 3.979656612793903 7 14 4.072910991030405 7 15 4.607742975948867 7 16 4.564108603117347 7 17 4.905935887364464 7 18 5.25654530529706 7 19 3.9735720541424087 7 20 3.8217608055235033 7 21 3.2995238319227056 7 22 3.45938201978462 7 23 2.965746506708278 7 24 3.099171935541591 7 25 3.4277208531434553 7 26 2.697668173524356 7 27 2.5875562796209977 7 28 2.10417333897756 7 29 2.849507227329809 7 30 2.781644804319818 7 31 2.224465190399144 7 32 2.925355330782324 7 33 2.064589490991054 7 34 2.444024348162131 7 35 2.4620266097914225 7 36 2.45281371009947 7 37 2.2807703352553403 7 38 3.0582564694196535 7 39 2.9993262653559114 7 40 5.5967072780951845 7 41 -0.413010668672932 7 42 4.0384703856832616 7 43 5.3867878285046125 7 44 5.148056500592798 7 45 4.0974583023748465 7 46 4.648518202700544 7 47 4.563231253287519 7 48 4.330712847163898 7 49 6.5513485689591135 7 50 4.602714027099061 7 51 5.112321989551003 7 52 5.327366093469511 7 53 5.398370079112123 7 54 5.379138711849574 7 55 5.058135519126061 7 56 5.2520975232657285 8 1 1.2549912605003115 8 2 1.626674461546834 8 3 1.5646810714411146 8 4 3.2607970509907673 8 5 1.6904240348622963 8 6 1.5746420222367945 8 7 1.477398298030046 8 8 1.2714352332705872 8 9 1.4726697555198314 8 10 1.8478029740014534 8 11 2.1658760506943224 8 12 4.324537063413658 8 13 4.262972850440235 8 14 4.582834630295125 8 15 4.606863769188749 8 16 4.83220153852821 8 17 5.1445021290811965 8 18 5.2759122764297715 8 19 4.546342695149264 8 20 4.226692504183525 8 21 3.3895936800153166 8 22 3.804511377149602 8 23 3.263170756533256 8 24 3.878092139387306 8 25 3.2579932444230266 8 26 3.147577810607345 8 27 3.014378598447422 8 28 2.638291445752337 8 29 2.9214475063965466 8 30 3.3704935319122598 8 31 2.481494774078463 8 32 2.8116527306097368 8 33 2.221454630443013 8 34 2.409751566124001 8 35 1.8047381485660665 8 36 1.9679149002849172 8 37 2.131557550937785 8 38 2.2534647925769775 8 39 2.824974680341505 8 40 4.745187390112637 8 41 -1.9015402767746608 8 42 3.5757959689718177 8 43 5.406529276590707 8 44 5.220558495089616 8 45 3.780048380363745 8 46 4.990733152471783 8 47 5.06613752007793 8 48 5.020108636740414 8 49 7.005760070286065 8 50 5.083892489789754 8 51 4.8738127334576085 8 52 4.654170502528597 8 53 5.103949785717571 8 54 6.0784418590841405 8 55 4.805151914703977 8 56 4.783170969593414 9 1 0.906229852416871 9 2 1.3950360434677407 9 3 1.1994552498919546 9 4 2.894885908678056 9 5 1.230206069429208 9 6 1.3403793270875255 9 7 0.6469065385943438 9 8 0.8711577619370559 9 9 0.9029007587625699 9 10 1.1775091218301554 9 11 1.6886420721565614 9 12 3.790789194594076 9 13 3.804827562438362 9 14 4.129286005951194 9 15 3.884920132642762 9 16 4.378502477919113 9 17 4.734730457901442 9 18 4.9122075326728805 9 19 3.964338215491587 9 20 3.946396311892549 9 21 2.9926926670777974 9 22 3.517462268066595 9 23 2.817143945447775 9 24 3.546915059188059 9 25 3.2074132290450947 9 26 2.3420417560491273 9 27 2.551268913694976 9 28 2.0518908841236154 9 29 2.3967592437682583 9 30 2.6341080139100512 9 31 1.862716985157256 9 32 2.237805239552337 9 33 2.2286755711711237 9 34 1.6626182127324967 9 35 1.7756872342478758 9 36 2.51531903895372 9 37 2.59496810822057 9 38 3.032925227839243 9 39 3.455152195134403 9 40 5.59287902919932 9 41 2.5453390064801127 9 42 4.837321058397826 9 43 6.522081043381068 9 44 5.1928615071579465 9 45 4.616591020687352 9 46 4.992855975324723 9 47 5.278252653966206 9 48 5.12090093440364 9 49 7.438180756944043 9 50 5.243020628057401 9 51 4.824870801855962 9 52 4.864898279749209 9 53 5.625345165652714 9 54 6.438318835649515 9 55 5.458814202003815 9 56 5.459198114981707 10 1 0.8782578244448445 10 2 1.225339073770769 10 3 1.3671977637883117 10 4 2.7849881141648822 10 5 1.1000285546363102 10 6 0.9889717602953496 10 7 0.8969155039879269 10 8 0.7913119667066439 10 9 0.938206478683675 10 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114 42 1.4630434353922759 114 43 -1.6081885672836336 114 44 -4.441667537577622 114 45 1.2051181774575461 114 46 -3.5621367721253705 114 47 -3.422090403156261 114 48 -4.203000246725917 114 49 -17.112103589060148 114 50 -3.9138762015422124 114 51 -5.150654720212508 114 52 -4.5525607951477625 114 53 -6.585381701015486 114 54 -8.48067133311617 114 55 -5.43099308738444 114 56 -2.576696251053338 115 1 0.2667180726836875 115 2 0.9799068921762313 115 3 0.8026780276818641 115 4 2.236749268140059 115 5 0.692279477145533 115 6 0.7999635974864461 115 7 0.49870461278810296 115 8 0.4290957081804265 115 9 0.5272593692125795 115 10 0.9664783132972161 115 11 -1.2859971121051075 115 12 -9.083618543566143 115 13 -5.630433032771137 115 14 -8.367777248705021 115 15 -9.60171713017957 115 16 -8.194337540421902 115 17 -2.130113752670841 115 18 -2.4524947534032364 115 19 -2.3610728196838515 115 20 -2.6913032011972575 115 21 2.5308157702023237 115 22 2.797621223502304 115 23 2.372756513407772 115 24 2.534032681843577 115 25 2.4837838921600124 115 26 2.2862981808618983 115 27 2.334932312459138 115 28 1.8032173322993401 115 29 2.1803025525741333 115 30 2.1847095554284266 115 31 1.8601405096883568 115 32 1.8426219386069036 115 33 1.6279528479675633 115 34 1.7819657967408844 115 35 1.9188999681503631 115 36 2.275543061312291 115 37 2.525588864132274 115 38 3.156366787698623 115 39 3.354494975544455 115 40 6.383365721048488 115 41 6.603014938269016 115 42 1.8522777168401454 115 43 -5.080857885416503 115 44 -4.587100937436912 115 45 1.4293489411659621 115 46 -3.184877162953402 115 47 -2.8248500338654097 115 48 -2.3485197953066184 115 49 -12.521654610640404 115 50 -2.4566562207820937 115 51 -2.3469015075831052 115 52 -1.7123523152419509 115 53 -3.1007951430876517 115 54 -5.683092112187776 115 55 -1.8009731295973133 115 56 -0.11690616677117305 116 1 0.5482115994833663 116 2 1.0306292638067234 116 3 1.0042290940399852 116 4 2.3268987459060213 116 5 0.8610455037888514 116 6 0.857127897943009 116 7 0.7937824788210008 116 8 0.6720002050220018 116 9 0.6122177781187048 116 10 0.9003453468828084 116 11 -1.7256493730344928 116 12 -13.994107004382954 116 13 -7.416123194757503 116 14 -3.6024761041681543 116 15 -5.325339620641763 116 16 -3.3056515688164225 116 17 -2.1441984358905515 116 18 -8.786799587977008 116 19 -2.2920753839467842 116 20 -3.007770772652634 116 21 1.8912885952695575 116 22 2.4559238065281335 116 23 1.8219278186801446 116 24 2.0786571111387175 116 25 1.816750306569915 116 26 2.211984590268365 116 27 2.2200283159615495 116 28 1.8077829711760671 116 29 2.40675824085983 116 30 2.3484596336071704 116 31 1.7148281074117975 116 32 2.021257250383748 116 33 1.5824715795955555 116 34 1.6391300971974516 116 35 1.7663200694700194 116 36 2.55209569124537 116 37 2.6496366469829837 116 38 2.5975325891871464 116 39 3.3181950193245555 116 40 6.945752361864049 116 41 4.748177237349633 116 42 -1.084091036677897 116 43 -7.939798407025108 116 44 -5.50938500773524 116 45 -0.2900081168113964 116 46 -3.999662327754205 116 47 -2.4457268867017348 116 48 -3.7493828886833143 116 49 -5.727008291295853 116 50 -3.072604685351486 116 51 -1.2041533682373036 116 52 -1.1785978590533226 116 53 -3.9988750730394926 116 54 -6.2904846945881765 116 55 -1.386938480776248 116 56 -0.31400417164952543 117 1 0.010526456713478183 117 2 0.687828836260536 117 3 0.5875089840995358 117 4 2.2085753377521016 117 5 0.5775060321137886 117 6 0.5589144302380191 117 7 0.5198475269199534 117 8 0.3686256440203195 117 9 0.2607290012061938 117 10 0.9114768173363146 117 11 -1.9332993959387537 117 12 -7.519408577142155 117 13 -8.891478210790485 117 14 -11.066806536295196 117 15 -8.570567899768346 117 16 -15.12450940970816 117 17 -13.472156902832072 117 18 -14.424270957651768 117 19 -5.62108744685715 117 20 -6.112748631867781 117 21 0.8692852359780616 117 22 1.1415093921137185 117 23 0.3994511046780076 117 24 0.7734196703080585 117 25 0.566360934146644 117 26 1.9393017379244952 117 27 2.1590571753293872 117 28 1.6667690759248828 117 29 2.2881447120768073 117 30 2.223371833943105 117 31 1.6449548443181925 117 32 1.8007350332513639 117 33 1.8256690143184144 117 34 1.6805562691320972 117 35 1.43758285768196 117 36 2.1984844528883656 117 37 2.6820996184290067 117 38 2.481056791355421 117 39 3.2438935992604243 117 40 6.732971784676696 117 41 5.838878107711519 117 42 0.7413937701628441 117 43 -6.111641436495258 117 44 -5.657743535754786 117 45 2.1221616801041634 117 46 -0.0490439127290081 117 47 -0.7132181067353258 117 48 -1.765660182926709 117 49 -2.609982791219508 117 50 -1.583995737420505 117 51 -3.155352024520706 117 52 -3.386980818283739 117 53 -5.091270858655616 117 54 -13.439499811399902 117 55 -4.157743183784341 117 56 -4.1758304046622 118 1 -0.026700853241109712 118 2 0.6512343905751763 118 3 0.4236715929894146 118 4 2.0387071224293436 118 5 0.5463095463718446 118 6 0.5446191432154563 118 7 0.4239127491669876 118 8 0.2959951622989294 118 9 0.3277381591244435 118 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1.9237529843242527 119 31 0.6425986419619889 119 32 -4.414857482341155 119 33 0.8996814883308843 119 34 0.34871288728871264 119 35 1.2434387135378202 119 36 1.6511178555217683 119 37 1.999701836031221 119 38 2.1489015592001834 119 39 2.678820834187661 119 40 6.0127015144064195 119 41 3.276404095237506 119 42 -1.6854600066909349 119 43 -8.148924873778697 119 44 -6.079919557930808 119 45 -3.808261050318567 119 46 -14.462765326450423 119 47 -11.320563914081134 119 48 -12.426436343702871 119 49 -10.161819243055959 119 50 -10.293074046498813 119 51 -7.269212038380722 119 52 -6.490723022025943 119 53 -9.109566076950834 119 54 -9.541266963167054 119 55 -6.963079289120447 119 56 -4.490210169041962 120 1 -0.5668669989183215 120 2 0.08156454087706777 120 3 -0.08585609081994505 120 4 1.5622372898596524 120 5 -0.042458491995816416 120 6 -0.025457506465528468 120 7 -0.0474399678286872 120 8 -0.2892187288499599 120 9 -0.2367503434234095 120 10 0.034638839462068916 120 11 -2.920626100882039 120 12 -8.890971850259831 120 13 -9.401000686634205 120 14 -14.70576933328908 120 15 -12.66765981033176 120 16 -17.870646738747045 120 17 -15.751856291339749 120 18 -16.576770607560743 120 19 -7.377400754983931 120 20 -13.761700692736941 120 21 -6.029305064166643 120 22 -3.7989984732646107 120 23 -3.4919739991823278 120 24 -9.922747999952879 120 25 -3.4487629980757326 120 26 1.2785166004554185 120 27 0.8772339012781387 120 28 0.5719877661176653 120 29 1.2729741010512754 120 30 1.160122055667415 120 31 -0.8363969738211878 120 32 -11.253319020802692 120 33 0.10259101403833881 120 34 0.1637985787992733 120 35 -0.5174421154777278 120 36 0.2890413310100115 120 37 0.3640302537275133 120 38 0.27959107693633456 120 39 1.050363281377777 120 40 3.3461212035255947 120 41 0.15003900954599558 120 42 -3.5246987472250133 120 43 -10.425369672503287 120 44 -8.33197216853264 120 45 -7.731616969011274 120 46 -14.238373138327665 120 47 -12.849380175539881 120 48 -11.602004299063573 120 49 -18.241173567488005 120 50 -10.99012466427482 120 51 -9.28399880601723 120 52 -9.47135275498728 120 53 -11.436110516966778 120 54 -9.735746835626202 120 55 -8.790381002950657 120 56 -5.126140818703185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 745.0 5 101.0 6 270.0 7 341.0 8 553.0 9 647.0 10 436.0 11 338.0 12 324.0 13 326.0 14 419.0 15 509.0 16 793.0 17 1022.0 18 1214.0 19 1460.0 20 1836.0 21 2426.0 22 3041.0 23 3717.0 24 4499.0 25 4888.0 26 5466.0 27 6234.0 28 7159.0 29 8347.0 30 9949.0 31 11814.0 32 13859.0 33 16265.0 34 19042.0 35 23131.0 36 29839.0 37 37745.0 38 38117.0 39 25249.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.27128375983554 0.39413057347416175 0.0676968880697526 0.266888778620543 8 99.23584036294038 0.636917842205997 0.0655702842560431 0.06167151059757567 9 96.04380803856242 2.2240731551711916 0.870489827745091 0.8616289785213015 10 36.83986673282767 34.17487772028071 18.289147231870704 10.696108315020911 11 27.82377543063727 34.09902885092507 22.796484015028 15.280711703409654 12 26.03742822712129 14.915290281420571 22.796484015028 36.250797476430144 13 21.377474334978164 14.701378254211333 25.565055867506096 38.3560915433044 14 19.839008102762595 19.70786102663349 35.3802910756187 25.072839794985217 15 31.030342642388764 21.28034441847413 24.44213158661258 23.247181352524528 16 35.02819248909535 22.286605908010923 22.37313379907089 20.31206780382283 17 23.530954600392008 21.90126143496645 33.30344262934227 21.264341335299267 18 22.11959353368375 22.172404382237122 34.32846930059297 21.379532783486155 19 33.1038960578182 24.09197832882146 22.06074884069129 20.743376772669052 20 23.13649942170282 34.42890492510519 22.02247908550993 20.412116567682055 21 22.361593535124406 22.384986177075213 33.84738073296945 21.406039554830937 22 20.52349897214149 21.755157014248244 25.271850854185868 32.4494931594244 23 19.48358970723754 24.72708584390728 24.515488764443184 31.273835684411992 24 19.182811248396174 25.559123549468698 34.47461880356421 20.783446398570913 25 30.802957480895465 26.688216863028654 22.400153119816256 20.108672536259622 26 19.881465517241377 25.139660843920147 24.39243988203267 30.58643375680581 27 19.458853416404704 22.443462032457443 25.229025780035908 32.86865877110195 28 19.540870281127347 32.513822153771905 26.657061541409682 21.288246023691066 29 31.073558507130343 23.21280412999851 25.732358509966886 19.98127885290426 30 20.643962892196654 22.573086562423565 37.834149246535766 18.948801298844014 31 19.89817985861465 31.977267731665638 29.61718179433182 18.507370615387888 32 30.864464680361976 21.88636274951489 28.162413931329528 19.0867586387936 33 19.137636630208277 23.22367416779379 29.818134054691132 27.820555147306802 34 18.38407120441127 33.186645626850584 31.191645538199676 17.237637630538465 35 18.594285227973874 23.64878761444792 41.284937199917735 16.471989957660476 36 19.399809958729843 33.14872856717392 29.600346045297897 17.851115428798344 37 19.01812602988806 22.980353997386217 40.52609523268367 17.475424740042047 38 28.720008530906764 24.909181388405077 28.907688479721322 17.463121600966836 39 19.512966027658017 23.07072185011826 28.636773657407076 28.779538464816646 40 14.987113310058758 18.336938750740718 33.46041537264309 33.215532566557435 41 13.95357984431262 16.715475411477385 31.757150570508074 37.57379417370192 42 26.20514640638864 23.291570541259983 30.660514640638866 19.842768411712512 43 15.328378992838468 19.37272649766966 38.87120609298624 26.427688416505628 44 16.63758102428948 21.080205142816443 28.097086687085977 34.18512714580811 45 17.643276806456427 24.78846823293715 38.94599990053922 18.622255060067207 46 17.09445712804599 24.70840538131877 30.83180360813944 27.3653338824958 47 16.27088954999486 32.0396852694322 32.164144968955995 19.525280211616945 48 18.064864250823582 33.02070316937408 30.27767522435533 18.636757355447006 49 15.049048592658027 26.767993388947858 26.10261378774818 32.08034423064593 50 26.715698454504793 24.69917152370067 30.175847111691667 18.409282910102867 51 18.393378980823787 33.412967584593616 30.775438521647008 17.41821491293559 52 18.352058595995658 24.249984030887813 38.70699872956841 18.690958643548125 53 18.507235879738115 23.99485890389559 38.47478448581938 19.023120730546918 54 17.697502335676706 30.290618572448608 28.377672706862807 23.634206385011883 55 18.020828521786093 34.281785525207766 29.681531830827772 18.01585412217836 56 26.76502965093569 24.493803487092077 29.562870636696143 19.17829622527609 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.5 11 3.0 12 9.0 13 82.0 14 149.0 15 388.0 16 627.0 17 961.0 18 1295.0 19 1516.0 20 1737.0 21 1883.5 22 2139.0 23 2248.0 24 2445.0 25 2642.0 26 2854.5 27 3067.0 28 3623.0 29 4813.0 30 5447.0 31 6003.0 32 6559.0 33 7098.5 34 7638.0 35 7736.5 36 7835.0 37 7366.0 38 6661.0 39 6425.0 40 6471.5 41 6518.0 42 6532.0 43 6546.0 44 6842.5 45 7139.0 46 7550.0 47 9450.5 48 10940.0 49 16828.0 50 22716.0 51 22109.0 52 21502.0 53 17860.0 54 13670.0 55 13122.0 56 13427.0 57 13732.0 58 13666.0 59 13600.0 60 12823.5 61 12047.0 62 12360.5 63 12973.0 64 13272.0 65 12255.5 66 11239.0 67 10341.0 68 9443.0 69 8573.0 70 7703.0 71 6594.0 72 4901.0 73 4317.0 74 3811.0 75 3305.0 76 3110.0 77 2915.0 78 2112.0 79 1003.5 80 698.0 81 561.0 82 424.0 83 375.5 84 327.0 85 232.5 86 138.0 87 85.0 88 24.0 89 16.0 90 11.0 91 6.0 92 4.0 93 2.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01559509463386971 14 0.00496207556532218 15 0.22045792868788544 16 0.053165095342737646 17 3.544339689515843E-4 18 3.544339689515843E-4 19 0.10314028496491104 20 0.09853264336854044 21 0.0 22 0.0 23 0.0 24 7.088679379031686E-4 25 0.0028354717516126744 26 0.00992415113064436 27 0.10420358687176579 28 0.12263415325724818 29 0.037570000708867936 30 0.015240660664918125 31 0.02622811370241724 32 0.086127454455235 33 0.15914085205926137 34 0.047848585808463885 35 0.0474941518395123 36 0.034025661019352095 37 0.19706528673708087 38 0.2870915148507833 39 0.19777415467498405 40 0.7109945417168781 41 0.8779329410930743 42 0.13822924789111787 43 0.22542000425320763 44 0.4820301977741547 45 0.22045792868788544 46 0.17686255050684058 47 0.042886510243141704 48 0.15736868221450345 49 0.49549868859431484 50 0.14815339902176225 51 0.302686609484653 52 0.12263415325724818 53 0.17296377684837316 54 0.2257744382221592 55 0.24774934429715742 56 0.1878500035443397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 282140.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7241431858622 #Duplication Level Percentage of deduplicated Percentage of total 1 85.3779032204436 56.1138953616912 2 8.73759461786349 11.485418395289578 3 2.752700939674594 5.427567321210914 4 1.1761639605766143 3.092094742199527 5 0.5740787688250983 1.8865417601112124 6 0.33569978376995024 1.3238148393575515 7 0.22078787031138875 1.01577655214331 8 0.14853385508333425 0.7809808287556133 9 0.11544633141631996 0.6828850094659843 >10 0.4872863840519698 5.697004663202494 >50 0.03978511320996029 1.7712715563468817 >100 0.030559579711998484 3.948033178010658 >500 0.0011531916872452258 0.4793877875008142 >1k 0.0017297875308678386 2.819482323324946 >5k 5.765958436226129E-4 3.4758456813893024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 9172 3.2508683632239315 No Hit CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 2697 0.9559084142624229 No Hit CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 2588 0.9172751116467003 No Hit CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 2155 0.7638052030906642 No Hit CGCACCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 633 0.22435670234635285 No Hit CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 632 0.2240022683774013 No Hit CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 474 0.16800170128305097 No Hit CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 435 0.15417877649393918 No Hit CGCACCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 386 0.13681151201531155 No Hit CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 385 0.13645707804635995 No Hit CGCACCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 330 0.11696320975402281 No Hit CGCACCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 322 0.11412773800241016 No Hit CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG 295 0.10455802084071739 No Hit CGCACCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC 286 0.10136811512015312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.544339689515843E-4 0.0 0.0 0.0 0.0 8 0.001063301906854753 0.0 0.0 0.0 0.0 9 0.001063301906854753 0.0 0.0 0.0 0.0 10 0.002126603813709506 0.0 0.0 0.0 0.0 11 0.0028354717516126744 0.0 0.0 0.0 0.0 12 0.004253207627419012 0.0 0.0 0.0 0.0 13 0.005670943503225349 0.0 0.0 0.0 0.0 14 0.008151981285886439 0.0 0.0 0.0 0.0 15 0.012050754944353866 0.0 0.0 0.0 0.0 16 0.014886226695966542 0.0 0.0 0.0 0.0 17 0.018785000354433968 0.0 0.0 0.0 0.0 18 0.02197490607499823 0.0 0.0 0.0 0.0 19 0.025164811795562486 0.0 0.0 0.0 0.0 20 0.02870915148507833 0.0 0.0 0.0 0.0 21 0.032607925143545755 0.0 0.0 0.0 0.0 22 0.035797830864110015 0.0 0.0 0.0 0.0 23 0.04005103849152903 0.0 0.0 0.0 0.0 24 0.046076415963705965 0.0 0.0 0.0 0.0 25 0.05068405756007656 0.0 0.0 0.0 0.0 26 0.05564613312539874 0.0 0.0 0.0 0.0 27 0.05812717090805983 0.0 0.0 0.0 0.0 28 0.06415254838023676 0.0 0.0 0.0 0.0 29 0.06663358616289786 0.0 0.0 0.0 0.0 30 0.06911462394555894 0.0 0.0 0.0 0.0 31 0.07159566172822003 0.0 0.0 0.0 0.0 32 0.0723045296661232 0.0 0.0 0.0 3.544339689515843E-4 33 0.07372226554192954 0.0 0.0 0.0 7.088679379031686E-4 34 0.07407669951088112 0.0 0.0 0.0 7.088679379031686E-4 35 0.07549443538668746 0.0 0.0 0.0 7.088679379031686E-4 36 0.07691217126249379 0.0 0.0 0.0 7.088679379031686E-4 37 0.07939320904515489 0.0 0.0 0.0 7.088679379031686E-4 38 0.08081094492096122 0.0 0.0 0.0 7.088679379031686E-4 39 0.08258311476571914 0.0 0.0 0.0 7.088679379031686E-4 40 0.08506415254838023 0.0 0.0 0.0 7.088679379031686E-4 41 0.08683632239313815 0.0 0.0 0.0 7.088679379031686E-4 42 0.08967179414475084 0.0 0.0 0.0 7.088679379031686E-4 43 0.09144396398950876 0.0 0.0 0.0 7.088679379031686E-4 44 0.09463386971007301 7.088679379031686E-4 0.0 0.0 7.088679379031686E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCGT 15 0.008202177 50.33925 35 CCAACGT 20 4.0880087E-4 50.222183 48 CAACGAT 440 0.0 50.195244 46 AAGCGTA 15 0.008348499 50.1146 43 CTTGAGG 20 4.1716974E-4 50.016388 33 TCGTTGA 15 0.008455047 49.95409 14 TGGTCGA 20 4.1974362E-4 49.95409 14 CGTATCG 15 0.008466949 49.93632 13 CACGGGA 15 0.008490788 49.900818 28 AAGCCGT 25 2.1080312E-5 49.900818 10 TTACGTA 15 0.008490788 49.900818 10 ACGGGAG 15 0.008496755 49.89195 29 CGTCTCA 445 0.0 49.889935 41 GCGTAAG 15 0.00852664 49.847656 25 GGAACGA 20 4.267935E-4 49.785778 8 CGGGATA 170 0.0 49.759304 6 CCGGTAT 15 0.008586643 49.759304 5 CGCACCG 28235 0.0 49.283478 1 CACCGGG 26945 0.0 49.223763 3 GCACCGG 28025 0.0 49.217766 2 >>END_MODULE