FastQCFastQC Report
Fri 17 Jun 2016
SRR1526249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53237
Sequences flagged as poor quality0
Sequence length56
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG8881.668012848207074No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2340.439543926216729No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2200.41324642635760844No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1740.3268403553919267No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1440.27048856997952553No Hit
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT610.11458196367188235No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG600.11270357082480228No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.10143321374232207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGGG150.00796744850.65229840
CGTCTCA251.9079092E-550.65229841
TGCTCGT203.9830673E-450.4108737
ATGCTCG204.0018733E-450.3628636
TAGCGCC150.00821186650.26711344
ACTGTCT150.00821186650.26711343
CCAAAAC309.916348E-750.26711343
CAAAAAC150.00821186650.26711343
CAGTTCT251.9962536E-550.2671144
ACAGTTC251.9962536E-550.2671143
TGTAGCG150.008242805550.2193742
ACGATTC150.008242805550.2193748
ATATGCC301.0047461E-650.17172634
TTCTAGC150.00827383250.17172634
GATTCAG150.00839880749.98204450
TTATCCG150.00843026949.93484513
GCGCGGA150.00843026949.93484530
ATGGACT252.0763096E-549.93484515
CGTCCTT150.00843026949.93484527
TCGTCCT150.00846181949.88773726