FastQCFastQC Report
Fri 17 Jun 2016
SRR1526248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29136
Sequences flagged as poor quality0
Sequence length56
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG580.19906644700713894No Hit
GACACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.19563426688632618No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC560.19220208676551345No Hit
GACACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.17160900604063703No Hit
GACACTGGGTGGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.10296540362438221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCA150.00800130150.5392739
TTTTAAT150.00800130150.5392739
CAGCATT150.00805678450.4512243
ACAATAC150.00805678450.4512243
TAATTTT150.008112553550.3634842
TGCCGCT150.00822495250.1889145
CCGCTCC150.00822495250.1889147
CGCTCCT150.00822495250.1889148
CCTCAGA150.00822495250.1889115
TTGATAT150.00822495250.1889149
GCCGCTC150.00822495250.1889146
TTAACCA150.00822495250.1889145
TTCGTGG251.9918882E-550.18890815
ATTCGTG251.9918882E-550.18890814
GGGTTGG150.00828158350.10207711
AAGAGTG354.9722075E-850.10207710
AGATGTG150.00828158350.10207713
AGCATGT204.0748165E-450.10207711
GAGATGT150.00828158350.10207712
AATTCGT252.0124848E-550.10207413