FastQCFastQC Report
Fri 17 Jun 2016
SRR1526247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45222
Sequences flagged as poor quality0
Sequence length56
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.1968068639157932No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA700.1547919154393879No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC640.14152403697315466No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.11498828004068817No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT520.11498828004068817No Hit
TGGATGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.10393171465216046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA150.00812265650.39373840
TAGAGCG150.00819483850.2812534
GCAAGCG204.11732E-450.05777749
TGCCTAG150.00834063250.05777743
CTAGCAA204.11732E-450.05777746
TTCCTTC150.0083773850.0022215
TTCGTGG204.1399905E-450.00221615
TAGCAAG204.1399905E-450.00221647
ACAGCCA150.00841424849.94678512
GGGTTGG204.16276E-449.94678511
ACATGGT150.00841424849.94678510
TGGTAGA252.0684241E-549.94678531
GCTGGTC150.00841424849.94678511
GAAGCCG150.00841424849.9467859
TGTTTTG252.0684241E-549.94678527
GAATGTA150.00841424849.9467859
GAATGGT150.00841424849.9467859
GCAACAG204.16276E-449.9467859
CTGGTCG150.00841424849.94678512
GAACCCC150.00841424849.9467859
Produced by FastQC (version 0.11.5)