##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526247_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45222 Sequences flagged as poor quality 0 Sequence length 56 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.30505063906948 39.0 38.0 40.0 35.0 40.0 2 37.84138251293618 39.0 38.0 40.0 35.0 40.0 3 37.83578789084959 39.0 38.0 40.0 35.0 40.0 4 36.74488081022511 39.0 37.0 40.0 31.0 40.0 5 37.85849807615762 39.0 38.0 40.0 35.0 40.0 6 37.79620538675866 39.0 38.0 40.0 35.0 40.0 7 38.10019459555084 39.0 38.0 40.0 35.0 40.0 8 37.99307859006678 39.0 38.0 40.0 35.0 40.0 9 37.94540268011145 39.0 38.0 40.0 35.0 40.0 10 37.447790898235375 39.0 38.0 40.0 33.0 40.0 11 36.14678696209809 38.0 35.0 40.0 30.0 40.0 12 34.35721551457255 38.0 33.0 39.0 22.0 40.0 13 34.18090752288709 38.0 33.0 39.0 22.0 40.0 14 34.37136791826987 38.0 33.0 39.0 23.0 40.0 15 34.2837335810004 38.0 33.0 39.0 22.0 40.0 16 33.68336208040334 38.0 32.0 39.0 21.0 40.0 17 33.64335942682765 38.0 31.0 39.0 21.0 40.0 18 33.08122152934413 37.0 30.0 39.0 18.0 40.0 19 34.004687983724736 38.0 32.0 39.0 23.0 40.0 20 34.13427093007828 38.0 32.0 39.0 24.0 40.0 21 34.880390075626906 38.0 33.0 39.0 26.0 40.0 22 34.53686258900535 38.0 33.0 39.0 24.0 40.0 23 35.066803768077484 38.0 34.0 39.0 26.0 40.0 24 34.61717747998762 38.0 33.0 39.0 24.0 40.0 25 34.89359161470081 38.0 33.0 39.0 26.0 40.0 26 35.4959532970678 38.0 35.0 39.0 28.0 40.0 27 35.440493565078945 38.0 35.0 39.0 28.0 40.0 28 35.4330193268763 38.0 35.0 39.0 28.0 40.0 29 35.20080934058644 38.0 35.0 39.0 27.0 40.0 30 35.19191986201406 38.0 35.0 39.0 27.0 40.0 31 35.50249878377781 38.0 35.0 39.0 29.0 40.0 32 35.21748264119234 38.0 35.0 39.0 27.0 40.0 33 35.37939498474194 38.0 35.0 39.0 28.0 40.0 34 35.37716155853346 38.0 35.0 39.0 29.0 40.0 35 35.24105523860068 38.0 35.0 39.0 28.0 40.0 36 34.41886692317898 38.0 34.0 39.0 27.0 40.0 37 34.15704745477865 37.0 33.0 39.0 27.0 40.0 38 33.887643182521785 37.0 33.0 39.0 26.0 40.0 39 33.3990977842643 37.0 32.0 39.0 23.0 40.0 40 29.992304630489585 35.0 25.0 39.0 8.0 40.0 41 26.552916721949494 32.0 15.0 38.0 5.0 39.0 42 31.059749679359605 35.0 29.0 38.0 11.0 40.0 43 29.55331475830348 34.0 27.0 38.0 5.0 39.0 44 29.429392773428862 35.0 26.0 38.0 4.0 39.0 45 30.63398345937818 35.0 29.0 38.0 4.0 40.0 46 30.28169917296891 35.0 28.0 38.0 4.0 40.0 47 29.769116801556763 35.0 27.0 38.0 4.0 40.0 48 29.897218168148246 35.0 27.0 38.0 4.0 40.0 49 27.187320330812437 34.0 15.0 38.0 4.0 40.0 50 29.488103135641943 35.0 27.0 38.0 4.0 40.0 51 29.718345053292644 35.0 27.0 39.0 4.0 40.0 52 29.47505638848348 35.0 27.0 39.0 4.0 40.0 53 29.085865286807305 35.0 26.0 39.0 4.0 40.0 54 28.07863429304321 35.0 18.0 39.0 4.0 40.0 55 28.44504887001902 35.0 23.0 39.0 4.0 40.0 56 27.20140639511742 34.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.19558823366661926 1 2 0.2341505833627835 1 3 0.29615647365471176 1 4 0.6989055642615938 1 5 0.13356685851725558 1 6 0.004509175687275047 1 7 -0.11961705001281331 1 8 -0.0460084214318357 1 9 -0.11955410241719022 1 10 0.325211840644954 1 11 -3.499489468632582 1 12 -13.38054353769904 1 13 -7.809600462678578 1 14 -6.549739573904027 1 15 -6.862833881065686 1 16 -24.000885610700998 1 17 -5.369483093329119 1 18 -13.938649576888174 1 19 -6.411626013185213 1 20 -7.04767954173953 1 21 -6.884103246672616 1 22 -2.1893587587296217 1 23 -3.6160048387185917 1 24 -4.085469709290063 1 25 -3.3322116565209114 1 26 2.260513832645252 1 27 2.213323639576025 1 28 2.4990509945807347 1 29 2.0651793088602446 1 30 2.1679625843466113 1 31 1.7938089740177503 1 32 2.5340419226889352 1 33 1.3480113712713475 1 34 1.6511548293962122 1 35 1.4438464901295731 1 36 1.2607926135628702 1 37 1.538786965334289 1 38 1.4577717166519903 1 39 2.2348990442903585 1 40 5.958180391019564 1 41 -11.548968410941312 1 42 -12.076893045080581 1 43 -17.957801881114314 1 44 -22.9679309119729 1 45 -13.445038010380944 1 46 -18.410532026317558 1 47 -23.660208968598397 1 48 -16.30489209115052 1 49 -20.564306392602337 1 50 -15.718122483875984 1 51 -13.587526265065499 1 52 -13.437554629628815 1 53 -13.17403190608142 1 54 -12.54884523167269 1 55 -12.713198873217737 1 56 -7.976258466767241 2 1 0.5253939091905266 2 2 0.9707577262199294 2 3 0.8854421879404271 2 4 0.5493519928330244 2 5 0.6090132870886862 2 6 0.48888417568727505 2 7 0.6906508071300408 2 8 0.6280987214253102 2 9 0.7666066118685251 2 10 0.3162832692163846 2 11 0.31747481708170255 2 12 -3.6417042519847556 2 13 -3.31183260553572 2 14 2.211421140381688 2 15 2.7286839760771713 2 16 2.581703675013287 2 17 3.4988204780994536 2 18 -1.614988862602459 2 19 2.0057847011005023 2 20 2.240266886831897 2 21 2.939557467613099 2 22 2.542784098413236 2 23 1.8549773041385507 2 24 1.64220886213851 2 25 1.8709133434790886 2 26 1.6935495469309672 2 27 2.8606450681474556 2 28 1.7646759945807347 2 29 2.13884002314596 2 30 2.0563554414894654 2 31 2.0415768311606044 2 32 2.806363351260366 2 33 1.6984577998427781 2 34 1.8297262579676428 2 35 1.7362572044152884 2 36 2.0665961849914396 2 37 1.239679822477143 2 38 2.0292002880805597 2 39 2.384452615718928 2 40 4.6211268195909945 2 41 -4.881557696655598 2 42 -7.224214473652008 2 43 -12.326105452542883 2 44 -13.512573769115757 2 45 -7.228520153238087 2 46 -11.504282026317558 2 47 -18.577619682884112 2 48 -10.068284948293378 2 49 -12.707163535459479 2 50 -9.314104626733126 2 51 -5.9759191222083565 2 52 -5.819251058200244 2 53 -5.285639048938563 2 54 -4.73634523167269 2 55 -4.952038158932023 2 56 -2.011972752481526 3 1 0.6125914898356868 3 2 0.6673303068650895 3 3 0.49834541374687547 3 4 0.756509250897544 3 5 0.27988022257255807 3 6 0.8956382079453391 3 7 0.5802677426139127 3 8 0.8508809794898298 3 9 0.3326348376749735 3 10 0.5441058498615448 3 11 1.8668699783720228 3 12 3.8487191351120202 3 13 1.3701230396255681 3 14 3.063739688768784 3 15 2.4499541373674916 3 16 4.083215771787479 3 17 4.314848703905902 3 18 4.888539363203989 3 19 3.46395002368115 3 20 3.570408015864153 3 21 3.221311499871163 3 22 3.3290744209938836 3 23 2.575239400912743 3 24 3.32063628149335 3 25 2.55488511767264 3 26 1.9062511598341914 3 27 1.7225402294377758 3 28 1.978889704258151 3 29 1.5818843779846716 3 30 2.1581699576184974 3 31 1.5783711859993161 3 32 0.8214843190023018 3 33 1.2040021546814899 3 34 1.3216617418386107 3 35 1.319422526995936 3 36 2.904801830152728 3 37 3.0088330482835914 3 38 3.1542002880805597 3 39 3.7740695512027997 3 40 7.753687303461962 3 41 8.790317303344402 3 42 3.8614710102189598 3 43 -0.5332627106073993 3 44 2.6910552631423066 3 45 4.299201620955465 3 46 2.1338228123921184 3 47 -3.3099785538518525 3 48 2.7129650517066217 3 49 5.897675174217941 3 50 2.4978913410088097 3 51 3.8819437810174513 3 52 4.27500297405782 3 53 5.058110951061437 3 54 5.027767671553118 3 55 6.009151357197009 3 56 7.032382086228154 4 1 1.0127450996667164 4 2 1.4722458214580243 4 3 1.5441723466705852 4 4 1.802080167436202 4 5 1.0899160648664648 4 6 1.1890329852110852 4 7 1.3664940610982939 4 8 1.4123249119015 4 9 1.5054458975828098 4 10 0.9918785073116254 4 11 2.2386057694626516 4 12 4.171043763888264 4 13 4.301510648432533 4 14 4.178435029270574 4 15 3.401055007823203 4 16 4.876098516283129 4 17 5.598770874924853 4 18 5.7597631215245215 4 19 4.929643828084629 4 20 4.823352204292213 4 21 3.3401031025337318 4 22 3.8027047333338686 4 23 3.637963415249665 4 24 3.698260449440099 4 25 3.5090581847489304 4 26 3.1156725628039865 4 27 3.0466569729093607 4 28 3.2669081374378806 4 29 3.3885920072729405 4 30 2.8604229018069276 4 31 2.2501581803669524 4 32 2.0360260496730618 4 33 1.6615034347634108 4 34 1.7146468928882754 4 35 2.031148077431162 4 36 2.3361894389596927 4 37 1.9197393462866685 4 38 2.27324790712818 4 39 2.9888672982586115 4 40 7.4165137243529 4 41 9.576031589058687 4 42 5.262392669205138 4 43 7.198944150631718 4 44 6.579688135646148 4 45 5.72956516422223 4 46 5.06367432288879 4 47 4.601695793306362 4 48 5.927250765992337 4 49 8.487280908984964 4 50 5.148941008187506 4 51 4.763664211124979 4 52 5.314429497355313 4 53 5.50255539550588 4 54 6.441234133406677 4 55 4.766959856940996 4 56 6.001916136407363 5 1 0.6670964953974234 5 2 0.48045600208200057 5 3 0.9544077051818078 5 4 0.9550631997295795 5 5 0.17420725260592462 5 6 0.6208884860321007 5 7 0.5263189105783184 5 8 0.46322803177013583 5 9 0.44386954290300906 5 10 0.42619706231982946 5 11 1.872431713633425 5 12 3.137929368704903 5 13 3.754438946188415 5 14 3.8757530369334106 5 15 4.007240010559933 5 16 4.603794192254668 5 17 4.701945478099454 5 18 5.2411533787768505 5 19 3.319901080410844 5 20 4.074857404073278 5 21 2.601734191751028 5 22 3.7539909949649584 5 23 3.009610924828209 5 24 3.3006140345523036 5 25 3.0126159296859853 5 26 1.5825581676206255 5 27 2.659136447457797 5 28 2.158533753201425 5 29 2.628602954180444 5 30 3.020795096661878 5 31 1.4871587277123268 5 32 2.613475420225882 5 33 1.9921000412220877 5 34 2.2176572924504043 5 35 2.6064080664842564 5 36 2.6856694608535108 5 37 2.4821367190288655 5 38 3.1714416673909014 5 39 3.5994310639947926 5 40 7.83933802648755 5 41 10.324800061965092 5 42 5.860914836692817 5 43 8.061286788836426 5 44 7.709408989504933 5 45 6.429346226072255 5 46 6.007571421958307 5 47 6.349643248150372 5 48 5.523309879292828 5 49 8.956629567988799 5 50 6.065744511197906 5 51 6.7061929467571595 5 52 7.01749463145493 5 53 6.454662675199366 5 54 7.196844423499723 5 55 5.87177649624039 5 56 5.906130695794335 6 1 0.4095704964921154 6 2 0.8690712182834233 6 3 0.4648072673055097 6 4 0.611603976960005 6 5 0.2962652712156668 6 6 0.8398266360047373 6 7 0.7791924737967051 6 8 0.761531261107848 6 9 0.5213189134558291 6 10 0.40457692001003664 6 11 2.65130418216107 6 12 4.361519954364454 6 13 3.7777011246230074 6 14 4.527641378476929 6 15 3.95661056337876 6 16 4.415781055965667 6 17 4.249564525718505 6 18 5.5375408993023 6 19 4.516945415386218 6 20 3.9820823630223714 6 21 3.1654999279305613 6 22 3.263022193651331 6 23 3.0189157962020445 6 24 2.8252445764242253 6 25 2.778899454590203 6 26 2.496624943756366 6 27 1.7450696713220566 6 28 2.5050033755331143 6 29 2.150496769177707 6 30 2.130264171648193 6 31 1.0120629422717187 6 32 2.655073668720682 6 33 1.471027244287221 6 34 1.143218321459706 6 35 1.8089258552089404 6 36 2.002856105626357 6 37 2.5070409335882573 6 38 2.114517748398022 6 39 2.5444228538141687 6 40 6.781593089432267 6 41 3.385555398582497 6 42 4.7544561612686245 6 43 5.976721928409496 6 44 5.500323056281065 6 45 4.8248032594603245 6 46 4.365261624476094 6 47 4.808044999655571 6 48 4.943123781865349 6 49 7.757122178826236 6 50 4.529893389139893 6 51 5.493822941283707 6 52 5.600143783069598 6 53 5.9469998399503226 6 54 6.107900800073342 6 55 5.766959856940996 6 56 6.938424072915296 7 1 0.7556022425238567 7 2 0.22700772621993082 7 3 0.3354421879404299 7 4 1.0608103261663544 7 5 0.4121382870886876 7 6 0.5795091756872779 7 7 0.6958591404633765 7 8 0.8551820547586431 7 9 0.9030649452018551 7 10 1.103783269216386 7 11 1.136224817081704 7 12 3.371837414681913 7 13 3.1975423944642785 7 14 4.32287947371502 7 15 3.819308976077174 7 16 4.501495341679956 7 17 4.485278811432785 7 18 4.875636137397542 7 19 3.9193263677671695 7 20 4.239225220165231 7 21 2.4218491342797677 7 22 2.467784098413233 7 23 2.1101856374718864 7 24 2.92762552880518 7 25 1.4177883434790886 7 26 1.8831328802642986 7 27 1.4752284014807913 7 28 2.3740509945807347 7 29 2.2877983564792927 7 30 2.000105441489467 7 31 1.73428516449394 7 32 1.7376133512603644 7 33 1.7765827998427781 7 34 1.563059591300977 7 35 0.6414655377486227 7 36 1.8155545183247739 7 37 0.579263155810473 7 38 1.3708669547472283 7 39 1.8896609490522636 7 40 4.378418486257662 7 41 2.4903173033444013 7 42 4.219535526347993 7 43 5.079102880790451 7 44 5.024926230884244 7 45 3.3287715134285776 7 46 4.597801307015775 7 47 5.3921719837825535 7 48 4.946298385039956 7 49 8.226169797873851 7 50 3.7140203732668766 7 51 4.780330877791645 7 52 4.458873941799759 7 53 4.124777617728107 7 54 4.409488101660642 7 55 3.3010868410679777 7 56 3.6796939141851404 8 1 0.7883706041057792 8 2 1.1905670482538255 8 3 1.1651032048895829 8 4 1.3732397046974327 8 5 0.9223077786141118 8 6 0.9557803621279533 8 7 0.7565936037402068 8 8 0.8998148231202237 8 9 0.8637994084786911 8 10 1.2283595404028276 8 11 2.370970579793564 8 12 4.290763968354227 8 13 4.996694936837159 8 14 4.781071564110505 8 15 4.894732704890728 8 16 4.8314388445048095 8 17 5.320589545896063 8 18 6.114619188245001 8 19 4.226388514659824 8 20 4.149959683442063 8 21 3.230606196426656 8 22 3.5576146068878103 8 23 2.15397094820635 8 24 3.1279080146808838 8 25 3.3627035977163757 8 26 1.9472571740496107 8 27 1.4300306613677947 8 28 1.581678113224804 8 29 1.8281938367052817 8 30 1.8577325601335346 8 31 0.6128162379402653 8 32 1.2867658936332447 8 33 0.026582799842778115 8 34 0.41870930881510304 8 35 -0.6215288125338674 8 36 1.3025601680422838 8 37 0.26033660213816034 8 38 0.38301384740259437 8 39 0.7809038869053708 8 40 4.3931077517943855 8 41 -3.6588352390284804 8 42 2.7805524755005315 8 43 4.646617005084238 8 44 3.3698414851215297 8 45 2.039223490829709 8 46 2.036501871987525 8 47 2.328895147624362 8 48 2.3316091195032307 8 49 5.669955108608317 8 50 3.5140203732668738 8 51 4.091347826944183 8 52 4.521585806206538 8 53 4.346246544281776 8 54 5.432086971717144 8 55 5.005324129203569 8 56 5.6384509763320345 9 1 0.15013776164954606 9 2 0.557335595072388 9 3 0.17232743384206373 9 4 0.5782966649641708 9 5 -0.09769777848508454 9 6 0.8221321265069506 9 7 0.34203400385135296 9 8 0.7956192132285906 9 9 0.596780792196391 9 10 0.6718160561016333 9 11 1.3681920301964539 9 12 3.86008878080213 9 13 3.3098374764314897 9 14 3.7157756485784077 9 15 3.270948320339464 9 16 4.543025396324765 9 17 3.8248962977715877 9 18 4.988750891495904 9 19 3.6387252748709926 9 20 3.7845804114220627 9 21 2.623215254498348 9 22 3.036636557429631 9 23 2.510458861515602 9 24 2.8486637801713 9 25 2.74975555659384 9 26 1.7063569239801453 9 27 1.469763920606475 9 28 1.3863460765479445 9 29 1.4864322362607112 9 30 1.9083021628009433 9 31 0.950132159029458 9 32 2.1408920397849585 9 33 2.0757631277116317 9 34 1.292841012066006 9 35 1.584361712612008 9 36 2.147794955483242 9 37 2.3467494946082894 9 38 3.1460035667690818 9 39 3.7489505665386034 9 40 6.806833786804109 9 41 5.06080910662309 9 42 5.349043723069304 9 43 6.923638399916136 9 44 6.461811476785879 9 45 4.1334163221717475 9 46 5.107910596633264 9 47 5.1582922023617925 9 48 4.540833904165641 9 49 7.534639743229043 9 50 4.481233488020969 9 51 4.38115054992279 9 52 4.512972302455495 9 53 3.9925371805696344 9 54 4.28926952242567 9 55 4.570758972215518 9 56 4.506469870469292 10 1 0.7046218503669976 10 2 1.416223412494439 10 3 0.8756382663718014 10 4 1.196104443813418 10 5 1.1552755419906475 10 6 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0.9163658817219158 115 5 0.48991606486646333 115 6 0.1795091756872722 115 7 0.4458591404633765 115 8 0.35518205475864306 115 9 0.35306494520185794 115 10 0.5537832692163818 115 11 -1.4026640718071874 115 12 -10.733718140873645 115 13 -6.241346494424608 115 14 -8.843787192951645 115 15 -9.252913246145052 115 16 -9.187393547208934 115 17 -1.909165633011657 115 18 -2.513252751491347 115 19 -3.375118076677275 115 20 -3.605219224279214 115 21 2.016293578724209 115 22 2.612228542857679 115 23 1.949074526360775 115 24 2.48873663991629 115 25 1.9122327879235357 115 26 1.92202176915319 115 27 1.6974506237030127 115 28 1.657384327914066 115 29 1.0044650231459613 115 30 2.288994330378351 115 31 1.4787296089383801 115 32 0.8487244623714787 115 33 0.9821383553983338 115 34 1.5241707024120856 115 35 1.1136877599708441 115 36 2.4599989627692196 115 37 2.3070409335882545 115 38 3.209755843636117 115 39 2.8174387268300407 115 40 7.08952959736877 115 41 5.490317303344401 115 42 -0.14713114031867391 115 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1.26778834347909 116 26 1.608890456021875 116 27 1.069167795420185 116 28 0.7785964491261907 116 29 1.7681013867823268 116 30 1.5637418051258294 116 31 1.1918609220696936 116 32 1.6830678967149098 116 33 1.3356737089336903 116 34 1.225180803422191 116 35 1.7035867498698352 116 36 1.191312094082349 116 37 1.1050207315680538 116 38 1.3269275608078317 116 39 2.124509433900748 116 40 5.8390245468637225 116 41 4.235771848798947 116 42 -1.1895553827429168 116 43 -8.05877590708834 116 44 -8.052346496388484 116 45 -1.0060769714199047 116 46 -4.903713844499375 116 47 -3.510858319247749 116 48 -4.532489493747924 116 49 -10.35716353545948 116 50 -4.3587068994603975 116 51 -1.7878509403901752 116 52 -1.6229442400184269 116 53 -3.7600708671203797 116 54 -9.578390686218146 116 55 -3.0489131589320237 116 56 -0.9960636615724354 117 1 -0.09439775747613766 117 2 -0.15077005155784917 117 3 0.46877552127376276 117 4 1.0996992150552458 117 5 -0.3823061573557567 117 6 0.3517313979094965 117 7 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1.9711633608303814 118 28 2.4167339214099997 118 29 2.136172340219133 118 30 2.741568856123614 118 31 1.5838786604288941 118 32 -1.8087281121542702 118 33 1.7582901169159513 118 34 1.128506745772519 118 35 1.4752053751469987 118 36 1.8870992337719272 118 37 2.3471493346722667 118 38 2.263956385641535 118 39 3.1112056644994155 118 40 6.43858108788368 118 41 4.900073400905377 118 42 -0.5877815468227396 118 43 -12.892035330591664 118 44 -12.203122549603563 118 45 -5.368382958116136 118 46 -12.187971050707802 118 47 -13.801323951176796 118 48 -10.835815436098255 118 49 -13.225456218386308 118 50 -8.90061377307459 118 51 -7.618449610013233 118 52 -7.777711424053901 118 53 -7.1370110001580755 118 54 -14.387259865819031 118 55 -6.053791207712511 118 56 -3.9845337280912823 119 1 -0.6167554810533744 119 2 -0.3790898347556819 119 3 -0.6877285437668874 119 4 -0.10423032424014878 119 5 -0.6634714690088757 119 6 -0.23024692187370022 119 7 -0.4728400465284963 119 8 -0.6562000590624919 119 9 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47 -17.484250780445088 119 48 -13.27483982634216 119 49 -12.957163535459479 119 50 -11.6567113340502 119 51 -9.4721081465986 119 52 -8.85088215576122 119 53 -11.893108561133687 119 54 -12.14335742679464 119 55 -9.639157061371048 119 56 -7.5942898256522575 120 1 -0.8073607204390996 120 2 -0.2526219034096968 120 3 -0.6145578120595729 120 4 0.36821773357376486 120 5 -0.5767506018001995 120 6 -0.3982686020905035 120 7 -0.48006678546255444 120 8 -0.7781512785746898 120 9 -0.5580461659092535 120 10 -0.2462167307836154 120 11 -3.2322937014368165 120 12 -10.548532955688458 120 13 -8.100605753683869 120 14 -11.74749089665535 120 15 -11.334394727626531 120 16 -14.298504658320045 120 17 -14.168424892270917 120 18 -13.59473423297283 120 19 -7.3454884470476465 120 20 -13.753367372427363 120 21 -6.887410124979493 120 22 -4.091475160846024 120 23 -3.3620365847503386 120 24 -9.852004100824452 120 25 -2.406285730594984 120 26 1.2849847321161505 120 27 0.2900432162956008 120 28 0.9388658093955513 120 29 1.3674279861089218 120 30 0.8075128488968701 120 31 -0.5064555762468004 120 32 -13.292016278369264 120 33 0.0636198368798162 120 34 -0.10545892721754058 120 35 -0.36038631410323063 120 36 2.304443407213661 120 37 1.8033372298845478 120 38 1.8764225103027812 120 39 3.306327615718928 120 40 6.289529597368773 120 41 0.06809508112218055 120 42 -4.028612621800157 120 43 -13.07089711920955 120 44 -11.65840710244909 120 45 -7.884191449534384 120 46 -15.026272767058298 120 47 -15.985605793995223 120 48 -11.824071985330416 120 49 -16.771978350274296 120 50 -10.078572219325718 120 51 -8.569669122208357 120 52 -8.315200132274317 120 53 -9.571518678568193 120 54 -10.516437824265282 120 55 -8.063727973746836 120 56 -5.220306085814858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 88.0 5 17.0 6 28.0 7 33.0 8 83.0 9 109.0 10 71.0 11 66.0 12 57.0 13 64.0 14 67.0 15 86.0 16 155.0 17 177.0 18 230.0 19 244.0 20 298.0 21 422.0 22 527.0 23 619.0 24 744.0 25 793.0 26 926.0 27 1046.0 28 1167.0 29 1342.0 30 1681.0 31 1998.0 32 2420.0 33 2609.0 34 3026.0 35 3584.0 36 4411.0 37 5863.0 38 6069.0 39 4094.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 98.69974791030914 1.012781389589138 0.08181858387510504 0.20565211622661536 8 98.27075317323427 1.2891955243023308 0.17911635929414885 0.26093494316925386 9 92.70708946972712 4.988722303303702 1.3776480474105524 0.926540179558622 10 34.456680376807746 45.979832824731325 9.975233293529698 9.588253504931227 11 29.10972535491575 34.92769006235903 19.771350227765247 16.191234354959974 12 37.38224757861218 17.765689266286323 21.58462695148379 23.26743620361771 13 23.84365253168757 18.417501714336275 26.197270334240276 31.541575419735878 14 22.59569668959112 24.53506114415869 27.540302072046174 25.32894009420402 15 27.095386934450577 21.278623838974973 26.21090199729556 25.41508722927889 16 28.499358321901134 23.47878036907554 25.00110634154976 23.020754967473557 17 26.449515722435983 26.507009862456325 22.23917562248463 24.804298792623058 18 23.948520631551016 28.23625668922206 22.515589757197823 25.299632922029105 19 23.24041888989749 29.375428963623882 22.184338122965883 25.19981402351274 20 25.672295876585288 27.87897567561586 22.4054359133264 24.043292534472453 21 26.2571314846756 25.018796161160495 24.481447083278052 24.24262527088585 22 26.283667241608065 25.987351289195527 24.275794967051432 23.453186502144973 23 27.81831851753571 27.097430454203707 23.218787315908187 21.865463712352394 24 25.403564636681264 27.816107204458007 25.122727875812657 21.657600283048072 25 25.958604219185354 27.52200256512317 26.394232895493342 20.125160320198134 26 23.998142250530783 25.04202052370842 30.517958244869074 20.44187898089172 27 23.420790435071403 23.23701981622938 31.387136056681058 21.955053692018154 28 23.521204739231536 24.068209500609015 34.06267301516997 18.34791274498948 29 21.368202132837734 24.071861586795876 35.47280853135095 19.087127749015444 30 18.743226206011812 23.368206852314703 38.37119285129725 19.517374090376236 31 18.10962661475845 23.865245089364713 41.565652096973984 16.459476198902852 32 17.76995772746387 22.380098709691698 43.604895646592745 16.245047916251686 33 17.18216865334338 21.779589666326938 45.85899765143794 15.179244028891745 34 15.208974046950019 22.70504679514127 49.175830254220415 12.910148903688299 35 15.445031969733844 22.93856058762362 49.61171707338658 12.004690369255957 36 15.64857629593575 23.440784088143545 47.72450717936238 13.186132436558331 37 15.385297311673057 23.071297178697282 48.33226213958024 13.211143370049422 38 15.216234198270126 23.036149922377465 48.78465291638944 12.962962962962962 39 15.90415392117746 21.472270248703285 48.18681562264486 14.436760207474396 40 12.032723300862665 17.730322551882484 43.65261585787209 26.584338289382757 41 11.119051341030394 16.64396257174122 40.704348020277365 31.532638066951023 42 14.774136403897254 22.174490699734278 49.01018600531444 14.04118689105403 43 12.627146814404433 19.53684210526316 48.01329639889197 19.82271468144044 44 13.916677782224001 20.521541950113377 46.40522875816993 19.156551509492687 45 14.605172757695971 22.792048048580487 48.89740918862614 13.705370005097405 46 14.301220565757703 23.31036927098332 48.37073300400948 14.017677159249494 47 14.076187947969206 22.398460313246616 48.39173524466862 15.133616494115564 48 14.814158507951976 24.254640499712046 48.21468125636822 12.716519735967749 49 12.256056901533674 20.2822849522116 42.613914203156256 24.847743943098465 50 13.470779724073786 25.05037978607968 47.43672077418783 14.042119715658702 51 13.637271214642263 25.930116472545755 47.38990571270105 13.042706600110925 52 13.7153738845464 25.825380306016253 46.58001372865969 13.879232080777662 53 13.507765248022688 26.011919268006288 46.704477479672995 13.775838004298027 54 13.221638632183144 24.58613124127385 43.90443897790484 18.287791148638167 55 14.67118974768303 25.007760188018267 46.4879606225888 13.833089441709904 56 14.05207756232687 27.503601108033244 44.85761772853186 13.586703601108033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 4.0 7 7.0 8 16.5 9 26.0 10 83.0 11 140.0 12 228.5 13 297.0 14 277.0 15 298.5 16 320.0 17 439.5 18 559.0 19 685.5 20 812.0 21 873.5 22 1044.5 23 1154.0 24 1404.0 25 1654.0 26 1992.0 27 2330.0 28 2584.0 29 3022.5 30 3207.0 31 3084.0 32 2961.0 33 2639.5 34 2318.0 35 1926.0 36 1534.0 37 1314.0 38 954.0 39 814.0 40 808.0 41 802.0 42 783.5 43 765.0 44 777.0 45 789.0 46 809.5 47 917.0 48 1004.0 49 1074.0 50 1144.0 51 1152.0 52 1160.0 53 1246.5 54 1433.0 55 1533.0 56 1578.0 57 1623.0 58 1639.5 59 1656.0 60 1787.0 61 1918.0 62 1808.5 63 1552.5 64 1406.0 65 1321.5 66 1237.0 67 1112.0 68 987.0 69 830.5 70 674.0 71 587.5 72 418.0 73 335.0 74 300.5 75 266.0 76 202.5 77 139.0 78 107.5 79 52.5 80 29.0 81 19.0 82 9.0 83 7.0 84 5.0 85 3.5 86 2.0 87 1.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.03316969616558312 14 0.0022113130777055416 15 0.24545575162531508 16 0.061916766175755165 17 0.0 18 0.0 19 0.12162221927380479 20 0.0906638361859272 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.013267878466233248 27 0.12604484542921587 28 0.14815797620627127 29 0.053071513864932994 30 0.024324443854760958 31 0.030958383087877583 32 0.08624121003051612 33 0.19459555083808766 34 0.05528282694263854 35 0.05086020078722746 36 0.05086020078722746 37 0.22334262084825968 38 0.2918933262571315 39 0.23882181239219846 40 0.7982840210517005 41 0.9796116934235549 42 0.13710141081774357 43 0.21449736853743753 44 0.53071513864933 45 0.22334262084825968 46 0.1746937331387378 47 0.03980363539869975 48 0.16805979390562115 49 0.5130246340276857 50 0.1437353500508602 51 0.3250630224227146 52 0.13489009774003805 53 0.18796161160497105 54 0.21891999469284862 55 0.265357569324665 56 0.21449736853743753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 45222.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.95687939498474 #Duplication Level Percentage of deduplicated Percentage of total 1 86.72448889282236 65.00597054530981 2 7.3546331533764056 11.02560700543983 3 2.569548927633714 5.77816107204458 4 1.1741452045903769 3.520410419707222 5 0.5870726022951884 2.2002565123170137 6 0.42481635542968404 1.9105744991375877 7 0.2684603357229253 1.40860643049843 8 0.1947074962386052 1.167573305028526 9 0.16225624686550433 1.094599973464243 >10 0.5251202171283594 6.16514086064305 >50 0.01475056789686403 0.723099376409712 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89 0.1968068639157932 No Hit TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 70 0.1547919154393879 No Hit TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 64 0.14152403697315466 No Hit TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52 0.11498828004068817 No Hit TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 52 0.11498828004068817 No Hit TGGATGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 0.10393171465216046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0022113130777055416 0.0 0.0 0.0 0.0 9 0.0022113130777055416 0.0 0.0 0.0 0.0 10 0.008845252310822166 0.0 0.0 0.0 0.0 11 0.015479191543938791 0.0 0.0 0.0 0.0 12 0.017690504621644332 0.0 0.0 0.0 0.0 13 0.026535756932466497 0.0 0.0 0.0 0.0 14 0.03316969616558312 0.0 0.0 0.0 0.0 15 0.03759232232099421 0.0 0.0 0.0 0.0 16 0.05086020078722746 0.0 0.0 0.0 0.0 17 0.05528282694263854 0.0 0.0 0.0 0.0 18 0.05970545309804962 0.0 0.0 0.0 0.0 19 0.0641280792534607 0.0 0.0 0.0 0.0 20 0.06633939233116624 0.0 0.0 0.0 0.0 21 0.07518464464198842 0.0 0.0 0.0 0.0 22 0.08181858387510504 0.0 0.0 0.0 0.0 23 0.10835434080757153 0.0 0.0 0.0 0.0 24 0.12604484542921587 0.0 0.0 0.0 0.0 25 0.13931272389544913 0.0 0.0 0.0 0.0 26 0.159214541594799 0.0 0.0 0.0 0.0 27 0.18796161160497102 0.0 0.0 0.0 0.0 28 0.19901817699349875 0.0 0.0 0.0 0.0 29 0.20786342930432092 0.0 0.0 0.0 0.0 30 0.21449736853743753 0.0 0.0 0.0 0.0 31 0.21670868161514306 0.0 0.0 0.0 0.0 32 0.22113130777055415 0.0 0.0 0.0 0.0 33 0.2233426208482597 0.0 0.0 0.0 0.0 34 0.2233426208482597 0.0 0.0 0.0 0.0 35 0.22555393392596523 0.0 0.0 0.0 0.0 36 0.2343991862367874 0.0 0.0 0.0 0.0 37 0.2388218123921985 0.0 0.0 0.0 0.0 38 0.24324443854760958 0.0 0.0 0.0 0.0 39 0.24324443854760958 0.0 0.0 0.0 0.0 40 0.2454557516253151 0.0 0.0 0.0 0.0 41 0.25208969085843175 0.0 0.0 0.0 0.0 42 0.2565123170138428 0.0 0.0 0.0 0.0 43 0.2609349431692539 0.0 0.0 0.0 0.0 44 0.265357569324665 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCCGA 15 0.008122656 50.393738 40 TAGAGCG 15 0.008194838 50.28125 34 GCAAGCG 20 4.11732E-4 50.057777 49 TGCCTAG 15 0.008340632 50.057777 43 CTAGCAA 20 4.11732E-4 50.057777 46 TTCCTTC 15 0.00837738 50.00222 15 TTCGTGG 20 4.1399905E-4 50.002216 15 TAGCAAG 20 4.1399905E-4 50.002216 47 ACAGCCA 15 0.008414248 49.946785 12 GGGTTGG 20 4.16276E-4 49.946785 11 ACATGGT 15 0.008414248 49.946785 10 TGGTAGA 25 2.0684241E-5 49.946785 31 GCTGGTC 15 0.008414248 49.946785 11 GAAGCCG 15 0.008414248 49.946785 9 TGTTTTG 25 2.0684241E-5 49.946785 27 GAATGTA 15 0.008414248 49.946785 9 GAATGGT 15 0.008414248 49.946785 9 GCAACAG 20 4.16276E-4 49.946785 9 CTGGTCG 15 0.008414248 49.946785 12 GAACCCC 15 0.008414248 49.946785 9 >>END_MODULE